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Foliar Expression Of Candidate Genes Involved In Condensed Tannin Biosynthesis In White Clover (Trifolium Repens), Stephen N. Panter, J. Simmonds, A. Winkworth, A. Mouradov, G. C. Spangenberg

IGC Proceedings (1977-2023)

Bloat disease in cattle and sheep is caused by the rapid microbial degradation of protein-rich fodder in the rumen. This leads to the production of protein foams that trap gases, causing bloat, a condition that is often fatal to livestock and costly to farmers. Condensed tannins (CTs) are phenolic polymers produced by the phenylpropanoid pathway of plants (Figure 1). CTs bind to proteins under acidic to neutral conditions, such as those present in the rumen, slowing their breakdown. A diet with a CT content of between 2% and 4% by dry weight, which is provided by some pasture legumes (e.g. …

Screening Of Perennial Grasses And A Mutant Maize Collection By Fourier-Transformed Infrared (Ftir) Spectroscopy For Improved Biofuel Traits, S. C. Thain, P. Morris, S. Hawkins, C. Morris, I. S. Donnison

IGC Proceedings (1977-2023)

Currently the potential of biomass crops, including grasses, is limited because most species have not been bred for this purpose. However traits such as lignification, phenolic cross-linking and carbohydrate accessibility, which are also important for nutritive quality in forage grasses, can affect potential biofuel quality in applications such as combustion, fast-pyrolysis or fermentation. A collection of Lolium and Festuca species known to exhibit a range of lignin, cell wall phenolic and carbohydrate concentrations have been used to test optimum characteristics for biofuel processing. This collection formed a “calibration” set for subsequent high through-put FTIR chemical screening of additional plant lines: …

Nutritive Value Of Alopecurus Pratensis, Festuca Rubra, Arrhenatherum Elatius And Lolium Perenne Grown In The South Of Belgium, A. Nivyobizi, A. G. Deswysen, D. Dehareng, A. Peeters, Y. Larondelle

IGC Proceedings (1977-2023)

In Europe, recent strategies have aimed at encouraging farmers to use production techniques more efficient in preserving the environment and maintaining natural areas. Those strategies have encouraged the use of secondary grass species in forage production systems. However, the nutritive value of those grasses is not well known. Therefore, the aim of the present study was to evaluate the energy and nitrogen values of Alopecurus pratensis (ALPR), Festuca rubra (FERU) and Arrhenatherum elatius (AREL) under moderate rates of nitrogen (N) application (60 kg N/ha per cut) and a hay-cutting regime (2 cuts/year: 25 May and 9 July). Lolium perenne cv. …

Towards A Comparative Map Of White Clover (Trifolium Repens) And Barrel Medic (Medicago Truncatula), M. Febrer, G. Jenkins, M. T. Abberton, D. Milbourne

IGC Proceedings (1977-2023)

Grassland is of pivotal importance to the Irish agricultural industry. This dependence of grass is reflected in the large proportion of land area under grass, approx. 80% of the total land acreage in Ireland. The presence of white clover (Trifolium repens L.) in grassland significantly improves the overall nutritional value of the forage by increasing the relative amounts of nitrogen present. Genetic improvement of white clover through breeding of varieties should increase the productivity of grasslands. Advances in plant biotechnology offer the possibility of developing tools that will radically enhance our ability to breed improved plant varieties. The objective …

Gene Discovery And Molecular Dissection Of Fructan Metabolism In Perennial Ryegrass (Lolium Perenne), J. Chalmers, A. Lidgett, X. Johnson, K. Terdich, N. Cummings, Y. Y. Cao, K. Fulgueras, M. Emmerling, T. Sawbridge, E. K. Ong, A. Mouradov, G. C. Spangenberg

IGC Proceedings (1977-2023)

Fructans are the main soluble carbohydrate stored in up to a third of the vegetation of the earth, including the economically important temperate grasses. Fructans are polymers of fructose attached to a sucrose precursor. Perennial ryegrass (L. perenne L.) accumulates fructans of the inulin series, inulin neoseries and levan neoseries. Four enzymes are required to produce fructans of this profile: 1-SST (sucrose:sucrose 1- fructosyltransferase), 1-FFT (fructan:fructan 1-fructosyltransferase), 6G-FFT (6-glucose fructosyltransferase) and 6-FFT (fructan:fructan 6-fructosyltransferase) or 6-SFT (sucrose:fructan 6-fructosyltransferase) (Figure 1). Fructan biosynthetic enzymes have evolved from invertases and thus it is argued that fructan metabolism is an extension …

Eating Biodiversity: Investigating The Links Between Grassland Biodiversity And Quality Food Production, A. Hopkins, H. Buller, C. Morris, J. D. Wood

IGC Proceedings (1977-2023)

Modern food production systems are generally detrimental to biodiversity, and the widespread loss of species-diverse grassland as a consequence of intensive farming methods is well documented. Since the 1980s, a range of policy measures and financial incentives for farmers have been introduced in Europe to halt (and in some cases, reverse) this trend, primarily to meet environmental objectives of species and habitat conservation and landscape protection. Biodiversity, where associated with agricultural production, has largely been regarded as a positive 'externality' to the process of food production; a ‘product’ which benefits wider society without necessarily conferring an agricultural benefit to the …

Qtl Analysis And Trait Dissection In Ryegrass (Lolium Spp.), T. Yamada, J. W. Forster

IGC Proceedings (1977-2023)

Key points

1. Molecular marker-based genetic analysis permits the dissection of complex phenotypes through resolution of the locations of pleiotropic and interacting genetic factors.
2. Several QTLs for agronomically important characters such as flowering time, winter hardiness and forage quality have been identified in perennial ryegrass by molecular marker-based map analysis.
3. Some QTLs were putatively orthologous to those for equivalent traits in cereals.
4. The identification of co-location between QTLs and functionally-associated genetic markers is critical for the future implementation of marker-assisted selection programs.

Application Of Molecular Technologies In Forage Plant Breeding, K. F. Smith, J. W. Forster, M. P. Dobrowolski, N. O. I. Cogan, N. R. Bannan, E. Van Zijll De Jong, M Emmerling, G. C. Spangenberg

IGC Proceedings (1977-2023)

Key points

1. A range of molecular breeding technologies have been developed for forage plant species including both transgenic and non-transgenic methodologies.
2. The application of these technologies has the potential to greatly increase the range of genetic variation that is available for incorporation into breeding programs and subsequent delivery to producers in the form of improved germplasm.
3. Further developments in detailing the phenotypic effect of genes and alleles both through research in target species and through inference from results from model species will further refine the delivery of new forage cultivars.

Variability In Quantity And Composition Of Water Soluble Carbohydrates Among Irish Accessions And European Varieties Of Perennial Ryegrass, S. Mcgrath, S. Barth, A. Frohlich, M. Francioso, S. A. Lamorte, T. R. Hodkinson

IGC Proceedings (1977-2023)

The objective of this study was to identify perennial ryegrass accessions displaying high fructose and glucose contents and an improved ratio between fructose and glucose fractions across different time points throughout the year. Fructose and glucose are the main constituents of the water soluble carbohydrate (WSC) fraction in perennial ryegrass. For animal nutrition the amount of WSC is crucial as it is the primary energy source available to metabolise the intake of plant protein. The ratio between fructose and glucose fractions is important since fructosan chains, which are an excellent energy source for ruminants, are built from fructose. Furthermore the …

Constraints On Dairy Cattle Production From Locally Available Forages In Bangladesh, M. A. S. Khan

IGC Proceedings (1977-2023)

The productivity of milk producing animals in Bangladesh is low because of low individual yield and poor fertility. The reasons for the low productivity are complex but, in order of priority, appear to be (a) the imbalanced nature of the nutrients that arise from the digestion of the forage resources, (b) the incidence of disease/parasitism, and (c) the often harsh climatic circumstances. Thus, the purpose of this study was to find out the practical constraints on dairy cattle production from locally available forages under small holding village conditions of Bangladesh.

Pasture Management In Deer Farms In Mauritius, P. Grimaud, P. Thomas, J. Sauzier

IGC Proceedings (1977-2023)

Rusa deer (Cervus timorensis russa) were introduced into Mauritius in 1639, and with a population of 65,000 deer now provide the first source of red meat in the island. From June to September, the hunting period, extensive ranches ensure the venison market, while intensive farms provide it for the rest of the year. A pasture survey was carried out over 3 years to advise deer farmers on their sward management.

An Evaluation Of Grazing Value Of Maize And Companion Crops For Wintering Lactating Ewes, E. A. Van Zyl, C. S. Dannhauser

IGC Proceedings (1977-2023)

Northwestern KwaZulu-Natal (KZN), in South Africa, is well known for its sheep production from natural rangeland in summer (October to May). During winter, however, the nutritional value of the rangeland cannot maintain young growing sheep or pregnant and lactating ewes. With this in mind, Lyle (1991) suggested the use of planted pastures for the winter. Crichton et al. (1998) and Esterhuizen & Niemand (1989) suggested the use of maize crop residues for both cattle and sheep during winter, whereas Moore (1997) evaluated grazing (not harvested) maize for this purpose. He found that the crude protein content of the crop was …

Production Per Animal And Use Of Intake Estimatives To Predicted Animal Productivity In Pennisetum Purpureum Cv. Mott And Cynodon Spp Cv. Tifton 85 Pastures, Fernando L. F. De Quadros, A. R. Maixner, Gilberto V. Kozloski, D. P. Montardo, A. Noronha, D. G. Bandinelli, M. Da S. Brum, N. D. Aurélio

IGC Proceedings (1977-2023)

Dairy production is a very important activity in southern Brazil, being an essential source of income to small household farms. Milk production from pastures is an alternative to reduce costs in dairy systems. Some C4 grasses, such as dwarf elephant grass (DEG) and Tifton 85, have presented high animal production per animal and per area. Although studies evaluating milk production from these pastures are rare in south Brazil, in vitro studies have demonstrated that the nutritional value of these forages is higher than production registered in grazing. So, it is possible that, in spite of a high intrinsic nutritional value, …

Ingestive Behaviour Of Steers In Native Pastures In Southern Brazil, C. E. Pinto, Paulo C. De F. Carvalho, A. Frizzo, J. A.S.F. Júnior, T. M. S. Freitas, Carlos Nabinger

IGC Proceedings (1977-2023)

The Campos biome, particularly its native pastures, is the main resource for livestock production in southern Brazil (Boldrini, 1997). It has a huge floristic diversity in which more than 400 grass and 150 legume species are found. These pastures are very heterogeneous with horizontal as well as vertical structure. Hodgson (1985) stressed the importance of sward structure upon diet selection, emphasizing the difficulties animals could have to access all pasture layers in temperate pastures. This experiment investigates how variation in herbage allowance along the growing season influences the ingestive behaviour of steers.

Translational Genomics For Alfalfa Varietal Improvement, G. D. May

IGC Proceedings (1977-2023)

Key points

1. Medicago truncatula is a model legume with available mapping, genome, and RNA, protein and metabolite profiling databases and genetically diverse populations.
2. Genomics resources developed for M. truncatula have application in the study and improvement of alfalfa making it an excellent model for this forage legume.

Protection Of Agrobiodiversity: Model Calculations In Rhineland-Palatia: Costs And Implications For Farmers, H. Bergmann

IGC Proceedings (1977-2023)

Biological conservation and production use the same areas of land in less favoured areas. Grassland in these areas makes an important contribution to the protection of agro-biodiversity. However, under existing market conditions and production needs, the use of low yielding grasslands is not economically efficient. The objective of this study was to analyze the economic consequences of different mowing strategies in a small region in Rhineland-Palatia (Germany) that served the protection of two butterfly species.

Managing Resources By Grazing In Grasslands Dominated By Dominant Shrub Species, D. Magda, Cyril Agreil, Michel Meuret, E. Chambon-Dubreuil, P. -L. Osty

IGC Proceedings (1977-2023)

The European natural grasslands are attracting new attention because of their environmental value as habitats for threatened fauna and flora species and their contribution to the diversity of landscapes. Those responsible for the implementation of the European agri-environmental policy are hence encouraging livestock farmers to adopt grazing practices that contribute to the conservation of grassland biodiversity especially by limiting encroachment by dominant shrubs. However, current scientific knowledge and technical information are often insufficient to connect flock feeding and the impact of grazing on shrub dynamics and livestock farmers are not very enthusiastic about restoring or conserving “plant mosaics” including shrubs …

Effect Of Urea-Treated Pennisetum Pedicellatum And Supplementation Of Concentrates With Urea On Milk Production Of “Mossi” Ewes, V. M. C. Bougouma-Yameogo, A. J. Nianogo

IGC Proceedings (1977-2023)

The “Mossi” sheep is a near parent of “Djallonke” sheep that live in sudano-sahelian area of Burkina Faso. However, there are few available results on dairy production from this breed. The treatment of straw with urea is a technique used in several developing countries to improve the nutritional value of gramineous forages (Sourabié et al., 1995). The aim of this study was to test the influence on the performance of “Mossi” ewes and on milk composition of treatment of Pennisetum pedicellatum (Pp) with urea in comparison with addition of urea to the concentrate feed.

Using The N-Alkane Technique To Estimate The Herbage Intake And Diet Composition Of Cattle Grazing A Miscanthus Sinensis Grassland, Y. Zhang, Y. Togamura, K. Otsuki

IGC Proceedings (1977-2023)

Plant wax alkanes are now widely used as marker substances (Dove & Mayes 1991) for the estimation of forage intake and diet composition of grazing herbivores. The objective of this study was to evaluate this method with cattle grazing a M. sinensis grassland in Japan.

Selecting Grassland Species For Saline Environments, M. E. Rogers, A. D. Craig, T. D. Colmer, R. Munns, S. J. Hughes, P. M. Evans, P. G. H. Nichols, R. Snowball, D. Henry, J. Deretic, B. Dear, M. Ewing

IGC Proceedings (1977-2023)

In Australia, around 5.7 million hectares of agricultural land are currently affected by dryland salinity or at risk from shallow water tables and this figure is expected to increase over the next 50 years (LWRA, 2001). Most improved grassland species cannot tolerate the combined effects of salt and waterlogging and, therefore, the productivity of sown grasslands in salt-affected areas is low. However, there is potential to overcome the lack of suitably adapted fodder species by introducing new, salt and waterlogging-tolerant species and by diversifying the gene pool of proven species. Potential species include exotic, naturalised and native Australian grass, legumes, …

Effects Of The Stage Of Growth And Inoculation On Proteolysis In Field Pea Silage, L. Cavallarin, G. Borreani, S. Antoniazzi, E. Tabacco

IGC Proceedings (1977-2023)

Ensiling legumes is a good way of providing home-grown protein in dairy farms but severe protein degradation can occur when conserving legumes. Peas (Pisum sativum L.) are legumes with a high crude protein and starch content, that provide a high forage yield in a short growing period. Very little information is available on the protein value of field pea silage. The aim of the study was to investigate the effect of stage of maturity and inoculant application on proteolysis in field pea silage in the Po Valley, NW Italy.

Grass Silage: Factors Affecting Efficiency Of N Utilisation In Milk Production, P. Huhtanen, K. J. Shingfield

IGC Proceedings (1977-2023)

Key points

1. Low efficiency of N utilisation for milk production in cows fed grass silage-based diets is mainly due to excessive N losses in the rumen.
2. The type and extent of in silo fermentation can alter the balance of absorbed nutrients.
3. There is very little experimental evidence that the capture of N in the rumen can be improved by a better synchrony between energy and N release in the rumen. Nitrogen losses in the rumen can be reduced by decreasing the ratio between rumen degradable N and fermentable energy.
4. Rapeseed meal has increased milk protein output more than isonitrogenous soybean …

Decision Support For Grassland Systems In Developing Countries, P. K. Thornton

IGC Proceedings (1977-2023)

Key points

1. Information flows in complex systems are often themselves highly complex, and decision support approaches based on linear input-output processes may have only limited impact.

2. How decisions are made, and how they can be appropriately supported, is often incompletely understood, in part because of inadequate understanding of the objectives and attitudes of all the decision makers involved.

3. Much of the developing world faces daunting problems in the coming 30 years, and appropriate information could play a critical role in dealing with these.

4. System complexity, household variability, and institutional intricacies have to be embraced rather than …

Breeding Milestones Correspond With Changes To Wheat Rhizosphere Biogeochemistry That Affect P Acquisition, Rebecca Kay Mcgrail, David A. Van Sanford, David H. Mcnear Jr.

Plant and Soil Sciences Faculty Publications

Breeding wheat (Triticum aestivum L.) has resulted in small gains in improved nutrient acquisition and use as numerous traits are involved. In this study, we evaluated the impact of breeding on P-acquisition and identified both plant and soil variables that could be used to inform the selection of germplasm with increased P acquisition efficiency. We previously screened a historic panel of winter wheat cultivars for root system architecture and root tip organic acid content when grown in P-deficient solution/agar and used these characteristics together with breeding history to develop a predicted P extraction potential (PEP). We tested the validity …

Effect On Sward Botanical Composition Of Mixed And Sequential Grazing By Cattle And Sheep Of Upland Permanent Pasture In The Uk, J. E. Vale, M. D. Fraser, J. G. Evans

IGC Proceedings (1977-2023)

Previous work has shown benefits of sequential grazing by cattle and sheep, with superior liveweight gains being recorded for lambs grazing swards previously grazed by cattle. A preliminary study of the effects of mixed sheep and cattle grazing also suggested that more extensively grazed swards offer scope for complementary grazing between sheep and cattle. The aim of this experiment was to directly compare these two approaches of integrating the grazing of cattle and sheep.

Effects Of Breed And Stage Of Growing Season On The Metabolic Profile Of Sheep Grazing Moorland, V. J. Theobald, M. D. Fraser, J. M. Moorby

IGC Proceedings (1977-2023)

Previous studies have shown that the diet of sheep grazing heather moorland is affected by season (Grant et al., 1987) and the proportion of Calluna vulgaris cover (Osoro et al., 2000). In order to investigate the impact of these factors on the associated nutrient supply of animals grazing heathland, blood samples were taken to monitor the metabolic status of different breeds of sheep at different stages of the growing season when grazing sites with different proportions of heather cover.

Nutritional Value Of Pasture Forage For Sheep In Krkonoše National Park, P. Homolka

IGC Proceedings (1977-2023)

This study describes the nutritional value of pastures in extreme mountain conditions in Krkonoše Mountains National Park. The Park performs important ecological and environmental functions. Extensive sheep grazing serves in the preservation of rare, protected and endangered species of plants (including endemics) and in the restoration of the biodiversity of meadows. The objective of this experiment was to estimate the production of plant biomass in these pastures and its digestibility by sheep.

Impact Of In Utero Drug Exposure On Neonates Requiring Ecmo: A Retrospective Cohort Study, Hallie Walther, Aric Schadler, Karen Garlitz, John Anthony Bauer, Lindsay Kohler, Erika Waldsmith, Hubert O. Ballard

UK CARES Faculty Publications

The incidence of in utero drug exposure (IUDE) and neonatal extracorporeal membrane oxygenation (ECMO) utilization have both increased over the past decade. However, there are no studies to date that examine the impact that IUDE has on neonates requiring ECMO. In this retrospective cohort study, we compared the clinic course and outcomes of neonates who were placed on ECMO with IUDE vs. neonates without IUDE. Analysis included data extracted from medical records from all neonatal ECMO runs between January 2014 and January 2021 at the University of Kentucky Children’s Hospital. A total of 56 neonatal patients were placed on ECMO …

Forage News [2023-03], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

No abstract provided.

Body Mass Index Percentiles Versus Body Composition Assessments: Challenges For Disease Risk Classifications In Children, Jody L. Clasey, Elizabeth Ann Easley, Margaret O'Bryan Murphy, Stefan G. Kiessling, Arnold Stromberg, Aric Schadler, Hong Huang, John A. Bauer

UK CARES Faculty Publications

Background: Identifying at-risk children with optimal specificity and sensitivity to allow for the appropriate intervention strategies to be implemented is crucial to improving the health and well-being of children. We determined relationships of body mass indexes for age and sex percentile (BMI%) classifications to actual body composition using validated and convenient methodologies and compared fat and non-fat mass estimates to normative cut-off reference values to determine guideline reliability. We hypothesized that we would achieve an improved ability to identify at-risk children using simple, non-invasive body composition and index measures.

Methods: Cross-sectional study of a volunteer convenience sample of 1,064 (537 …