Digital Commons Network^™

Optimization Of Solid-State Fermentation Parameters For The Production Of Xylanase By Trichoderma Longibrachiatum On Wheat Bran, Elizabeth R. Ridder, Sue E. Nokes, Barbara L. Knutson

Biosystems and Agricultural Engineering Faculty Publications

Solid-state fermentation has the potential to produce inexpensive enzymes for use in high-volume industrial applications. Process parameters such as substrate moisture content and length of fermentation can have a significant effect on the amount and timing of enzyme production. This study was conducted in two stages, a screening stage and an optimization stage, to determine the effects of moisture content of the substrate, surfactant addition upon inoculation, depth of the substrate, and duration of fermentation on xylanase activity produced by Trichoderma longibrachiatum. Screening fermentations were conducted at 25°C, 50 and 75% wet basis moisture content (w.b.), 0.0 and 0.2% …

Response Of Runoff Diazinon Concentration To Formulation And Post-Application Irrigation, Joshua R. Evans, Dwayne R. Edwards, Stephen R. Workman, R. Michael Williams

Biosystems and Agricultural Engineering Faculty Publications

Pesticides used in urban environments can be transported in runoff to downstream waters and cause adverse environmental consequences. This experiment assessed the effects of post-application irrigation depth (0, 6.4, and 12.7 mm) and formulation (liquid and granular) on concentration and transport of diazinon (a pesticide commonly used for lawn insect control) in runoff from “tall” fescue (Festuca arundinacea Schreb.) plots. The post-application irrigation was applied using rainfall simulators immediately following diazinon application. The rainfall simulators were again used approximately 2 h after diazinon application to apply the equivalent of a heavy rainfall (64 mm/h for approximately 1.5 h) to generate …

A Bibliography Of Kentucky Printing History, James D. Birchfield, William J. Marshall

The Kentucky Review

No abstract provided.

Perjury! The Charges And The Defenses, Richard H. Underwood

Law Faculty Scholarly Articles

Perjury is the most hotly debated topic in America today. In this witty and instructive article, the author explains what constitutes the crime of perjury, provides examples of how defendants have sometimes avoided conviction, and discusses the impact of federal and state statutes on prosecutors, defendants, witnesses, the judiciary, the legislature, and society.

A Role For Nima In The Nuclear Localization Of Cyclin B In Aspergillus Nidulans, L. Wu, S. A. Osmani, Peter M. Mirabito

Biology Faculty Publications

NIMA promotes entry into mitosis in late G2 by some mechanism that is after activation of the Aspergillus nidulans G2 cyclin-dependent kinase, NIMX^CDC2/NIME^{Cyclin B}. Here we present two independent lines of evidence which indicate that this mechanism involves control of NIMX^CDC2/NIME^{Cyclin B} localization. First, we found that NIME^{Cyclin B} localized to the nucleus and the nucleus-associated organelle, the spindle pole body, in a NIMA-dependent manner. Analysis of cells from asynchronous cultures, synchronous cultures, and cultures arrested in S or G2 showed that NIME^{Cyclin B} was predominantly nuclear during interphase, with maximal …

The Snare Machinery Is Involved In Apical Plasma Membrane Trafficking In Mdck Cells, Seng Hui Low, Steven J. Chapin, Christian Wimmer, Sidney W. Whiteheart, László G. Kömüves, Keith E. Mostov, Thomas Weimbs

Molecular and Cellular Biochemistry Faculty Publications

We have investigated the controversial involvement of components of the SNARE (soluble N-ethyl maleimide–sensitive factor [NSF] attachment protein [SNAP] receptor) machinery in membrane traffic to the apical plasma membrane of polarized epithelial (MDCK) cells. Overexpression of syntaxin 3, but not of syntaxins 2 or 4, caused an inhibition of TGN to apical transport and apical recycling, and leads to an accumulation of small vesicles underneath the apical plasma membrane. All other tested transport steps were unaffected by syntaxin 3 overexpression. Botulinum neurotoxin E, which cleaves SNAP-23, and antibodies against α-SNAP inhibit both TGN to apical and basolateral transport in …

Evaluation Of Condition-Responsive Work Zone Traffic Controls At The I-75 Clays Ferry Bridge, Jerry G. Pigman, Kenneth R. Agent, Joel Weber

Kentucky Transportation Center Research Report

The objective of this study was to document the effectiveness of the application of advanced technologies for the real-time control and management of traffic in the work zone at the I 75 Clays Ferry Bridge reconstruction project. A description is given for each of the condition-responsive traffic control devices used on the project. A summary of the usage of each system is given along with a rating of its performance and effectiveness. The evaluation includes an analysis of accident data in the vicinity of the Clays Ferry Bridge for the period of January 1990 through June 1997. The possibility of …

Intelligent Transportation Systems Strategic Plan (Phase I Report), Joseph D. Crabtree, Ted H. Grossardt, Donald G. Hartman, Jerry G. Pigman, Jennifer R. Walton, Joel M. Weber, Study Advisory Committee

Kentucky Transportation Center Research Report

This interim report on an Intelligent Transportation Systems Strategic (ITS) Plan has been developed as documentation of the process of offering a vision for ITS and recommending an outline for organizational structure, infrastructure, and long-term planning for ITS in Kentucky. This plan provides an overview of the broad scope of ITS and relationships between various Intelligent Vehicle Highway Systems (IVHS) functional areas and ITS user service areas. Three of the functional areas of ITS have been addressed in this interim report with sections devoted to mission, vision, goals, and potential technology applications. Within each of the three areas, recommendations have …

The Enforcement Of Prisoners’ Rights In The United States: An Access To The Courts Issue, Roberta M. Harding

Law Faculty Scholarly Articles

This article examines how the development and status of the rights of incarcerated people is significantly effected by their ability to access the judiciary; specifically the federal judicial system. The relatively recent explosion in the American prison population provided the impetus for researching this topic. The objective was to examine whether this tremendous rise in the number of people incarcerated in U.S. penal facilities had impacted the posture of the rights afforded to these individuals. One conclusion reached was that the rise in the prison population had harshly eroded the right of access to the courts. The exploration of the …

Identity: Cultural Knowledge--Self-Knowledge. Disclosure Interviews Linda Alcoff, Ann M. Ciasullo, Christine R. Metzo, Jeffery L. Nicholas

disClosure: A Journal of Social Theory

No abstract provided.

The Decentralization Of Subject In African American Feminist Photography: Constructing Identity Based On Representation And Race In The Work Of Lorna Simpson, Carrie Mae Weems And Clarissa Sligh, Kathleen D. Adrian

disClosure: A Journal of Social Theory

No abstract provided.

The Canvas Of The Other: Fanon And Recognition, Anthony Peter Spanakos

disClosure: A Journal of Social Theory

No abstract provided.

Book Review: Daniels, Jessie. White Lies: Race, Class, Gender, And Sexuality In White Supremacist Discourse. New York: Routledge, 1997. White Trash: Race And Class In America. Edited By Matt Wray And Annalee Newitz. New York: Routledge, 1997., Meredith Redlin

disClosure: A Journal of Social Theory

No abstract provided.

(Re) Presenting Power. Disclosure Interviews Jane Flax, Susan Mains, Meredith Redlin

disClosure: A Journal of Social Theory

No abstract provided.

Southeastern Law Librarian Spring 1998, Seaall

Newsletters

No abstract provided.

The Ultraviolet-Optical Albedo Of Broad Emission Line Clouds, Kirk Korista, Gary J. Ferland

Physics and Astronomy Faculty Publications

We explore the effective UV-optical albedos of a variety of types of broad emission line clouds, as well as their possible effects on the observed spectra of active galactic nuclei (AGNs). An important albedo source in moderately ionized ionization-bounded clouds is that which is due to neutral hydrogen: Rayleigh scattering of continuum photons off the extreme damping wings of Lyα. The photons resulting from this scattering mechanism may contribute significantly to the Lyα emission line, especially in the very broad wings. In addition, line photons emitted near 1200 Å (e.g., N V λ1240) that stream toward the neutral portion of …

Validating A Vegetative Filter Strip Performance Model, Puneet Srivastava, Thomas A. Costello, Dwayne R. Edwards, James A. Ferguson

Biosystems and Agricultural Engineering Faculty Publications

Vegetative filter strips (VFS) reduce losses of nutrients, solids, and other materials from land area treated with fertilizers and manures. A number of models are available that simulate nutrient and sediment transport in VFS. While VFS effectiveness is considered to depend on lengths of pollutant source area and VFS areas, few published studies have tried to validate these models using variable pollutant source area and VFS area. The objective of this study was to validate an event-based nutrient transport model (Chaubey et al., 1995) that simulates soluble nutrient transport in VFS. This model links three sub-models: modified Green-Ampt infiltration, non-linear …

Lack Of Effect Of Leptin On Glucose Transport, Lipoprotein Lipase, And Insulin Action In Adipose And Muscle Cells, Subramanian Ranganathan, Theodore P. Ciaraldi, Robert R. Henry, Sunder Mudaliar, Philip A. Kern

Clinical and Translational Science Faculty Publications

The effect of leptin on glucose transport, lipogenesis, and lipoprotein lipase activity was studied in cultured rat adipocytes and 3T3-L1 adipocytes. Leptin had no effect on basal and insulin stimulated glucose transport in isolated adipocytes from the rat and the genetically obese mouse. The incorporation of glucose into lipids was also unaffected. Lipoprotein lipase (LPL) activity remained unchanged in response to leptin in these cells, as well as in minced adipose tissue. Leptin also had no effect on both basal and insulin-stimulated glucose transport in cultured rat and human skeletal muscle cells. These studies showed that leptin had no effect …

The Evolution Of Chutzpah As A Legal Term: The Chutzpah Championship, Chutzpah Award, Chutzpah Doctrine, And Now, The Supreme Court, Jack Achiezer Guggenheim

Kentucky Law Journal

No abstract provided.

United States V. Tucker: Should Independent Counsels Investigate And Prosecute Ordinary Citizens?, Hanly A. Ingram

Kentucky Law Journal

No abstract provided.

The Hemp Controversy: Can Industrial Hemp Save Kentucky?, Susan David Dwyer

Kentucky Law Journal

No abstract provided.

Personal Jurisdiction And The Internet--Proposed Limits On State Jurisdiction Over Data Communications In Tort Cases, David Wille

Kentucky Law Journal

No abstract provided.

The Role Of Ethics And Unauthorized Practice Opinions In Regulating The Practice Of Law In Kentucky, William H. Fortune

Law Faculty Scholarly Articles

The purpose of this article is to discuss the role of ethics and unauthorized practice opinions in regulating the practice of law, with suggestions for clarification and improvement.

The Kentucky Bench and Bar, the quarterly journal of the Kentucky Bar Association ("KBA"), prints "Advisory Ethics Opinions" and "Unauthorized Practice Opinions" over the signatures of the respective chairs of the Ethics and Unauthorized Practice of Law ("UPL") Committees. This article describes: 1) how ethics and unauthorized practice opinions are generated; 2) the legal effect of the opinions; 3) the relationship of ethics opinions to attorney discipline; 4) the Board of Bar …

In The Belly Of The Beast: A Comparison Of The Evolution And Status Of Prisoners' Rights In The United States And Europe, Roberta M. Harding

Law Faculty Scholarly Articles

Another epidemic has hit the international community. This one, however, is not derived from an unknown bacterial agent. Instead, it originates from a variety of social agents. The epidemic? The explosion in the number of people incarcerated in the global community. As of June 1997, the United States' prison population exceeded 1,700,000. This figure is consistent with the United States' status as one of the world's largest jailers. Like the United States, Europe's prison population has escalated. The growth in France's prison population is representative of the epidemic's trans-Atlantic scope.

The Article is divided into several sections. The first section …

Fecal Coliform Transport Through Intact Soil Blocks Amended With Poultry Manure, S. W. Mcmurry, Mark S. Coyne, E. Perfect

Plant and Soil Sciences Faculty Publications

Poultry production in Kentucky increased almost 200% between 1991 and 1995. Their waste is typically land applied, and fecal pathogen runoff and infiltration may cause nonpoint source groundwater pollution. We looked at the preferential flow of fecal coliforms through undisturbed soil blocks since fecal bacteria typically infiltrate the soil profile to contaminate groundwater. Poultry manure was uniformly distributed on top of sod-covered or tilled (upper 12.5 cm) soil blocks and the blocks were irrigated. Drainage was collected in 100 uniformly spaced cells beneath each block and analyzed for fecal coliform content and drainage volume. The spatial distribution of drainage and …

Method And Apparatus For Flotation Separation, Xiang-Huai Wang

Center for Applied Energy Research Faculty Patents

A flotation method and apparatus for separating a selected constituent from an effluent of an industrial process is provided. The apparatus includes a conditioning tank and a flotation cell. The conditioning tank allows mixing a flotation reagent with an effluent and includes a mechanism for also dissolving gas in the effluent within the conditioning tank. The flotation cell includes a dissolved gas flotation chamber having an overflow for recovering the selected constituent and a tailings drain. In addition, the flotation cell also includes an added gas flotation chamber having a sparger for injecting gas into the effluent, an overflow for …

Nicotinic Receptor Antagonists In The Treatment Of Neuropharmacological Disorders, Peter A. Crooks, Linda P. Dwoskin, Alain Ravard

Pharmaceutical Sciences Faculty Patents

Nicotine analogs that have nicotinic receptor antagonist properties. These compounds have been shown to competitively inhibit dopamine release induced by nicotine. The nicotine analog compounds are useful in the treatment of nicotine abuse, smoking cessation therapy, as an antidote for nicotine intoxication, treatment of cognitive disorders such as Alzheimer's disease and for the treatment of Parkinson's disease.

Permeable, Non-Irritating Prodrugs Of Nonsteroidal And Steroidal Agents, Paul Ashton, Thomas J. Smith, Peter G. Glavinos, John D. Conklin, Peter A. Crooks, Robert M. Riggs, Tadeusz Cynkowski, Grazyna Cynkowska

Ophthalmology and Visual Science Faculty Patents

Prodrugs containing an active drug molecule linked to a polyethylene glycol group, and a method of use thereof are described. Exemplary soluble ester prodrugs contain naproxen, triamcinolone acetonide, gancyclovir, taxol, cyclosporin, dideoxyinosine, trihydroxy steroids, and flurbiprofen molecules linked to polyethylene glycol (PEG) groups. Pharmaceutical compositions containing these prodrugs, and a method of using these esters for treating disease states or symptoms are also described.

Roadway Incident Emergency Response: Workshop Summary And Detour Maps, Jerry G. Pigman, Kenneth R. Agent, David H. Cain

Kentucky Transportation Center Research Report

Delays associated with incidents and accidents on heavily traveled highways have been recognized as a major inconvenience and a burden on the driving public. Heavy traffic volumes on interstates in Kentucky result in significantly increased probability for delay occurrences. When there is an occurrence which disrupts traffic, the adverse effects are widespread. High volumes of trucks use the north-south corridors in the state and delays can produce significant costs to both shippers and users of products being shipped.

Many efforts are underway to address the problems associated with congestion; including improved traffic management techniques and applications of advanced technology. A …

Identification Of A Gal/Galnac Lectin In The Protozoan Hartmannella Vermiformis As A Potential Receptor For Attachment And Invasion By The Legionnaires' Disease Bacterium, Chandrasekar Venkataraman, Bradley J. Haack, Subbarao Bondada, Yousef Abu Kwaik

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The Legionnaire's disease bacterium, Legionella pneumophila, is a facultative intracellular pathogen which invades and replicates within two evolutionarily distant hosts, free-living protozoa and mammalian cells. Invasion and intracellular replication within protozoa are thought to be major factors in the transmission of Legionnaire's disease. Although attachment and invasion of human macrophages by L. pneumophila is mediated in part by the complement receptors CR1 and CR3, the protozoan receptor involved in bacterial attachment and invasion has not been identified. To define the molecular events involved in invasion of protozoa by L. pneumophila, we examined the role of protein tyrosine phosphorylation …