Digital Commons Network^™

Angiotensin Ii Promotes Atherosclerotic Lesions And Aneurysms In Apolipoprotein E-Deficient Mice, Alan Daugherty, Michael W. Manning, Lisa A. Cassis

Gill Heart & Vascular Institute Faculty Publications

Increased plasma concentrations of angiotension II (Ang II) have been implicated in atherogenesis. To examine this relationship directly, we infused Ang II or vehicle for 1 month via osmotic minipumps into mature apoE^–/– mice. These doses of Ang II did not alter arterial blood pressure, body weight, serum cholesterol concentrations, or distribution of lipoprotein cholesterol. However, Ang II infusions promoted an increased severity of aortic atherosclerotic lesions. These Ang II–induced lesions were predominantly lipid-laden macrophages and lymphocytes; moreover, Ang II promoted a marked increase in the number of macrophages present in the adventitial tissue underlying lesions. Unexpectedly, pronounced abdominal …

Host-Derived Signals For Inducing Isoprenoid Gene Expression And Uses Thereof, Joseph Chappell, Marcos Lusso

Pharmaceutical Sciences Faculty Patents

Disclosed is a method for preparing a composition that is capable of activating the expression of a gene involved in the synthesis of an isoprenoid, the method involving: (a) contacting a plant cell with an elicitor under conditions that allow an elicitor-induced release of a compound that activates the synthesis of an isoprenoid; and (b) recovering a composition including the compound, wherein the compound is diffusible and has a molecular weight less than or equal to 10,000 daltons. Also disclosed is a substantially pure elicitor-induced composition, the composition being capable of activating a gene involved in the synthesis of a …

Novel Function Of Phosphoinositide 3-Kinase In T Cell Ca2+ Signaling. A Phosphatidylinositol 3,4,5-Trisphosphate-Mediated Ca2+ Entry Mechanism., A L Hsu, T T Ching, G Sen, D S Wang, Subbarao Bondada, K S Authi, C S Chen

Microbiology, Immunology, and Molecular Genetics Faculty Publications

This study presents evidence that phosphoinositide (PI) 3-kinase is involved in T cell Ca(2+) signaling via a phosphatidylinositol 3,4, 5-trisphosphate PI(3,4,5)P(3)-sensitive Ca(2+) entry pathway. First, exogenous PI(3,4,5)P(3) at concentrations close to its physiological levels induces Ca(2+) influx in T cells, whereas PI(3,4)P(2), PI(4, 5)P(2), and PI(3)P have no effect on [Ca(2+)](i). This Ca(2+) entry mechanism is cell type-specific as B cells and a number of cell lines examined do not respond to PI(3,4,5)P(3) stimulation. Second, inhibition of PI 3-kinase by wortmannin and by overexpression of the dominant negative inhibitor Deltap85 suppresses anti-CD3-induced Ca(2+) response, which could be reversed by subsequent …

A Recommended Master Plan For The Kentucky Transportation Cabinet To Build A Partnership With The Public, Theodore Hopwood Ii, Donald G. Hartman, Lenahan O'Connell

Kentucky Transportation Center Research Report

Public involvement with proposed projects is necessary to facilitate their timely implementation by state highway agencies. New procedures are necessary to involve the public in the project development process and adequately address their concerns. Kentucky Transportation Center (KTC) researchers and Kentucky Transportation Cabinet Study Advisory Committee members cooperated to identify a set of recommended actions to be considered for implementation by the Cabinet. That work consisted of a series of “internal” reviews of problems and proposed solutions. That was supplemented by external reviews and interviews conducted by KTC researchers to identify “best practices” of other transportation agencies related to promoting …

Solute Transport As Related To Soil Structure In Unsaturated Intact Soil Blocks, L. Bejat, E. Perfect, V. L. Quisenberry, Mark S. Coyne, G. R. Haszler

Plant and Soil Sciences Faculty Publications

Concern about soil and groundwater pollution has resulted in numerous studies focused on solute transport. The objectives of our study were to investigate the effect of soil type and land-use management on solute movement. Transport of water and Cl⁻ were measured through intact blocks of Maury (fine, mixed, semiactive, mesic Typic Paleudalf) and Cecil (fine, kaolinitic, thermic Typic Kanhapludult) soils, under steady-state, unsaturated flow conditions. Three replicate blocks for the Maury soil and two replicate blocks for the Cecil soil were studied per land-use treatment. The land-use treatments were conventional-till corn (Zea mays L.) production and long-term grass …

Contesting Masculinity: Editor's Preface, Christine Metzo

disClosure: A Journal of Social Theory

No abstract provided.

Rant #6, Bruce Burris

disClosure: A Journal of Social Theory

No abstract provided.

(Un)Settling Accounts: Disclosure Interviews Peter Jackson, Paul Kingsbury, Jennifer Kopf, Matt Mccourt, Theresa Zawacki

disClosure: A Journal of Social Theory

No abstract provided.

Better Loving Through Chemistry: How New Impotence Treatment Technologies Promise To Change Male Sexuality, Chris Weinke

disClosure: A Journal of Social Theory

No abstract provided.

Reimagining Masculinities: Disclosure Interviews Bell Hooks, Mary Curran, Susan Mains, Christine Metzo, Sarah Moore

disClosure: A Journal of Social Theory

No abstract provided.

Novel Function Of Phosphoinositide 3-Kinase In T Cell Ca2+ Signaling, Ao-Lin Hsu, Tsui-Ting Ching, Goutam Sen, Da-Sheng Wan, Subbarao Bondada, Kalwant S. Authi, Ching-Shih Chen

Pharmaceutical Sciences Faculty Publications

This study presents evidence that phosphoinositide (PI) 3-kinase is involved in T cell Ca²⁺ signaling via a phosphatidylinositol 3,4,5-trisphosphate PI(3,4,5)P₃-sensitive Ca²⁺entry pathway. First, exogenous PI(3,4,5)P₃ at concentrations close to its physiological levels induces Ca²⁺ influx in T cells, whereas PI(3,4)P₂, PI(4,5)P₂, and PI(3)P have no effect on [Ca²⁺]_i. This Ca²⁺ entry mechanism is cell type-specific as B cells and a number of cell lines examined do not respond to PI(3,4,5)P₃ stimulation. Second, inhibition of PI 3-kinase by wortmannin and by overexpression of the …

10th Biennial Midwest/Midsouth Securities Law Conference, Office Of Continuing Legal Education At The University Of Kentucky College Of Law

Continuing Legal Education Materials

Materials from the UK/CLE 10th Biennial Midwest/Midsouth Securities Law Conference held in February 2000.

Use Of Yeast Poly (A) Binding Proteins And Their Genes For Broad Range Protection Of Plants Against Bacterial, Fungal And Viral Pathogens, Arthur G. Hunt, Qing-Shun Li, Jianjun Yang, Carol Von Lanken

Plant and Soil Sciences Faculty Patents

Plants that accumulate the yeast polyadenylate binding protein (yPAB) display a range of abnormalities, including a characteristic chlorosis in leaves to a necrosis and pronounced inhibition of growth. The severity of these abnormalities reflects the levels of yeast PAB expression in the transgenic plants. In contrast, no obvious differences are seen in undifferentiated callus cultures that express the same range of yeast PAB. The expression of the yeast PAB1 gene in plants does not affect expression of the plant PAB gene family or alter poly(A) length in the total RNA population. It is proposed that the yeast PAB1 gene or …

Escherichia Coli Pathogen O157:H7 Does Not Survive Longer In Soil Than A Nonpathogenic Fecal Coliform, D. N. Mubiru, Mark S. Coyne, John H. Grove

Agronomy Notes

Survival rates for individual types of fecal organisms are quite different. Although some pathogens may persist as long as 5 years in soil, most fecal pathogens from human and animal waste usually die very quickly. Two to three months is sufficient in most cases to reduce pathogens to negligible numbers once they have been excreted or land-applied in animal wastes.

It is expensive and time- consuming to test for individual pathogens. Consequently, nonpathogenic fecal indicator bacteria, which are easily and inexpensively detected, are often used to study pathogen survival in soil and water. Current methods for rapidly detecting fecal indicator …

Root Growth And Development Of Float Tobacco Transplants Before And After Transplanting, L. V. Caruso, Robert C. Pearce, Lowell P. Bush

Agronomy Notes

In the production of float tobacco transplants, the seedling produces at least two different kinds of roots. The “media” roots are those that grow in the soilless medium within the float tray cell. They have a normal branched appearance similar to roots produced on soil-bed grown transplants. The “water” roots grow through the soilless medium in tray cells and into the nutrient solution below the float tray. They tend to be very fragile and less branched than roots growing in the soilless medium. In removal of seedlings from tray cells during transplanting, “water” roots are usually badly damaged or destroyed, …

Evaporation Reduction Potential In An Undisturbed Soil Irrigated With Surface Drip And Sand Tube Irrigation, Masoud Meshkat, Richard C. Warner, Stephen R. Workman

Biosystems and Agricultural Engineering Faculty Publications

The efficiency of drip irrigation is highly dependent on evaporation losses occurring from the constantly saturated soil beneath emitters. Advent of subsurface drip irrigation is in part an approach to curb this inefficiency. An irrigation method, Sand Tube Irrigation (STI), is proposed to increase the efficiency of “Normal” surface applied drip Irrigation (NI method) on permanent tree crops without the need for burying the irrigation tubing. The sand tube consists of removing a soil core beneath the emitter and filling the void with coarse sand. A weighing lysimeter was constructed in the laboratory and instrumented to directly measure temporal evaporation …

1998-1999 Monitoring Strategy, Kentucky River Basin Management Unit, L. Ormsbee, G. Epp, E. Caudill, Lee Colten

KWRRI Research Reports

During the fall of 1997 and spring of 1998 about 40 individuals and more than 30 organizations and agencies provided input into the development of a monitoring strategy for the Kentucky River basin under the Kentucky Watershed Management Framework. In addition to the agencies that were able to commit resources to the monitoring effort, citizen input was sought in the design of the strategy. Citizen monitoring efforts are also providing valuable information in many portions of the state. The intent of the coordinated planning process was to carefully consider agency resources and capabilities, taking into account where and when each …

The Future Of Kentucky's Punitive Damages Statute And Jural Rights Jurisprudence: A Call For Separation Of Powers, M. Scott Mcintyre

Kentucky Law Journal

No abstract provided.

Law In A Shrinking World: The Interaction Of Science And Technology With International Law, Joseph W. Dellapenna

Kentucky Law Journal

No abstract provided.

The Internet Is Changing The Public International Legal System, Henry H. Perritt Jr.

Kentucky Law Journal

No abstract provided.

Online Service Provider Copyright Liability: Is The Digital Millennium Copyright Act The Answer?, Justin Williamson

Kentucky Law Journal

No abstract provided.

Reporting On Child Pornography: A First Amendment Defense For Viewing Illegal Images?, Clay Calvert, Kelly Lyon

Kentucky Law Journal

No abstract provided.

Acting Without "Just Cause": An Analysis Of The Ninth Circuit's Decision In United States V. Symington, James R. Coltharp Jr.

Kentucky Law Journal

No abstract provided.

Scaling The Wall Between Church And State: An Analysis Of The Constitutionality Of School Vouchers, Allison M. Olczak

Kentucky Law Journal

No abstract provided.

Method And System For Gas Metal Arc Welding, Yuming Zhang, Liguo E

Electrical and Computer Engineering Faculty Patents

An improved method of gas metal arc welding (GMAW) is disclosed. The method includes utilizing a pulsed current having a variable waveform to ensure the detachment of one-droplet-per-pulse of current. During the welding process, the current is sufficient to produce a droplet at the end of a consumable electrode wire. After the droplet reaches a desired size, the current is lowered to induce an oscillation in the droplet. The current is then increased which, in combination with the momentum created by the oscillation, effects droplet detachment. The oscillation may be monitored by observing the arc voltage to determine a preferred …

Method For Testing Field Programmable Gate Arrays, Charles E. Stroud, Miron Abramovici

Electrical and Computer Engineering Faculty Patents

A method of testing field programmable gate arrays (FPGAs) includes the step of configuring programmable logic blocks of the FPGAs for completing a built-in self-test. This is followed by the steps of initiating the built-in self-test, generating test patterns with the programmable logic blocks and analyzing a resulting response to produce a pass/fail indication with the programmable logic blocks. More specifically, the configuring step includes establishing a first group of programmable logic blocks as test pattern generators and output response analyzers and a second group of programmable logic blocks as blocks under test. The blocks under test are then repeatedly …

Method For Testing Field Programmable Gate Arrays, Charles E. Stroud, Miron Abramovici

Electrical and Computer Engineering Faculty Patents

A method of testing field programmable gate arrays (FPGAs) includes the step of configuring programmable logic blocks of the FPGAs for completing a built-in self-test. Specifically, the FPGA under test may be configured to act as an iterative logic array wherein a first group of programmable logic blocks are configured as test pattern generators, output response analyzers and helper cells, and a second group of programmable logic blocks are configured as blocks under test. The blocks under test are then repeatedly reconfigured in order to completely test each block under test in all possible modes of operation. The first and …

Method Of Destroying Hazardous Organic Compounds, Dibakar Bhattacharyya, Susan Mawhinney

Chemical and Materials Engineering Faculty Patents

The present invention relates to a method of destroying hazardous vapor phase or aqueous phase organic compound(s) by providing a polymeric siloxane based solvent in a vessel and adding the hazardous organic compound(s) and ozone to the solvent. The solvent may also comprise a mixture of polymeric siloxane based solvent and fluorinated hydrocarbon solvent.

Enhanced Apoptosis Mediates Inhibition Of Ebv-Transformed Lymphoblastoid Cell Line Proliferation By Curcumin., D Ranjan, T D Johnston, K S Reddy, G Wu, Subbarao Bondada, C Chen

Microbiology, Immunology, and Molecular Genetics Faculty Publications

BACKGROUND: Epstein-Barr virus (EBV)-associated B-cell lymphomas occur more frequently in immunodeficient states such as organ transplantation and HIV infection. We have previously reported that B cell immortalization with EBV was promoted by cyclosporin A (CyA) and that curcumin (Cur), a natural phenol with known antioxidant and antitumor properties, blocked EBV-induced B cell immortalization. In the following experiments we show that Cur inhibits the proliferation of EBV-transformed lymphoblastoid cell lines (LCL) via enhanced apoptosis.

METHODS: LCL were generated by infecting freshly isolated human B cells with EBV (B95-8) for 12 h and coculturing with predetermined optimal concentrations of CyA (500 ng/ml) …

Optimization Of Solid-State Fermentation Parameters For The Production Of Xylanase By Trichoderma Longibrachiatum On Wheat Bran In A Forced Aeration System, Elizabeth R. Ridder, Sue E. Nokes, Barbara L. Knutson

Biosystems and Agricultural Engineering Faculty Publications

The effect of aeration on the production of xylanase by Trichoderma longibrachiatum on wheat bran in a solid-state fermentation (SSF) system has not been investigated. This study was conducted to investigate the interactive effects of aeration, initial moisture content of the substrate, and depth of the substrate on xylanase activity produced in a tray fermenter. The experiment was conducted as a split plot experiment with factorial treatments and three replications of each treatment combination. The whole plot treatment was aeration rate (0, 2.9, 5.7 L/min/kg bran). Initial moisture content (45, 55, 65% w.b.) and depth of substrate (1.0 and 2.5 …