Digital Commons Network^™

Methods For Screening For Genes And Small Molecules That Activate Mammalian Receptor Proteins, Deane L. Falcone, John M. Littleton

Plant and Soil Sciences Faculty Patents

Methods for screening mutations that affect the synthesis of plant small molecules or compounds capable of activating a mammalian nuclear receptor protein and systems for rapidly assigning functionality to genes that regulate plant secondary metabolism are provided.

Vampirism, Vampire Cults And The Teenager Of Today, Megan White, Hatim A. Omar

Pediatrics Faculty Publications

The aim of this paper is to summarize the limited literature on clinical vampirism, vampire cults and the involvement of adolescents in vampire-like behavior. The various definitions of clinical vampirism are outlined and the emergence of vampire cults from Gothic culture is examined. Further, the clinical features of cult behavior, the attraction to cults, the risk factors for cult involvement, and the clinical management of cult members are discussed. Additionally, vampire popular media is briefly introduced and a case study presented.

A Brain-Specific Cytochrome P450 Responsible For The Majority Of Deltamethrin Resistance In The Qtc279 Strain Of Tribolium Castaneum, Fang Zhu, R. Parthasarathy, Hua Bai, Katharina Woithe, Martin Kaussmann, Ralf Nauen, Douglas A. Harrison, Subba R. Palli

Entomology Faculty Publications

Cytochrome P450-mediated detoxification is one of the most important mechanisms involved in insecticide resistance. However, the molecular basis of this mechanism and the physiological functions of P450s associated with insecticide resistance remain largely unknown. Here, we exploited the functional genomics and reverse genetic approaches to identify and characterize a P450 gene responsible for the majority of deltamethrin resistance observed in the QTC279 strain of Tribolium castaneum. We used recently completed whole-genome sequence of T. castaneum to prepare custom microarrays and identified a P450 gene, CYP6BQ9, which showed more than a 200-fold higher expression in the deltamethrin-resistant QTC279 strain when compared …

Individual Micrornas (Mirnas) Display Distinct Mrna Targeting "Rules", Wang-Xia Wang, Bernard R. Wilfred, Kevin Xie, Mary H. Jennings, Yanling Hu, Arnold J. Stromberg, Peter T. Nelson

Pathology and Laboratory Medicine Faculty Publications

MicroRNAs (miRNAs) guide Argonaute (AGO)-containing microribonucleoprotein (miRNP) complexes to target mRNAs.It has been assumed that miRNAs behave similarly to each other with regard to mRNA target recognition. The usual assumptions, which are based on prior studies, are that miRNAs target preferentially sequences in the 3'UTR of mRNAs,guided by the 5' "seed" portion of the miRNAs. Here we isolated AGO- and miRNA-containing miRNPs from human H4 tumor cells by co-immunoprecipitation (co-IP) with anti-AGO antibody. Cells were transfected with miR-107, miR-124,miR-128, miR-320, or a negative control miRNA. Co-IPed RNAs were subjected to downstream high-density Affymetrix Human Gene 1.0 ST microarray analyses using …

Polarized Light Based Scheme To Monitor Column Performance In A Continuous Foam Fractionation Column, Janakiraman N. Swamy, Czarena L. Crofcheck, M.P. Mengüç

Biosystems and Agricultural Engineering Faculty Publications

BACKGROUND: A polarized light scattering technique was used to monitor the performance of a continuously operated foam fractionation process. The S11 and S12 parameters, elements of the light scattering matrix, combined together (S11+S12) have been correlated with the bubble size and liquid content for the case of a freely draining foam. The performance of a foam fractionation column is known to have a strong dependence on the bubble size distribution and liquid hold up in foam. In this study the enrichment is used as a metric, representative of foam properties and column performance, and correlated to the S11+S12 parameter.

RESULTS: …

Andy Warhol: Folk Artist In The Age Of Mechanical Reproduction, T. D. Richardson

disClosure: A Journal of Social Theory

No abstract provided.

After The Garden Is Gone: Megachurches, Pastoral, And Theologies Of Consumption, Andrew Battista

disClosure: A Journal of Social Theory

No abstract provided.

Consumed With (And By) Collecting: Museology As A Narrative Strategy, Gretchen E. Henderson

disClosure: A Journal of Social Theory

No abstract provided.

Consumption And The Construction Of Community In Jacques Tati’S Mon Oncle, Jennifer Spohrer

disClosure: A Journal of Social Theory

No abstract provided.

Global Ky (Spring 2010), University Of Kentucky International Center

Global Kentucky

No abstract provided.

Celebrity Culture And The Rise Of The Ordinary: An Interview With Joshua Gamson, David Hoopes, Drew Heverin

disClosure: A Journal of Social Theory

No abstract provided.

Consuming And Maintaining Difference: American Fans Resisting The Globalization Of Japanese Pop Culture, Laura Beltz Imaoka

disClosure: A Journal of Social Theory

No abstract provided.

Much Ado About Mutton: An Interview With Deborah Gewertz And Frederick Errington, Rebecca Lane, Christine Smith

disClosure: A Journal of Social Theory

No abstract provided.

Boundaries Of The Self, W. Hunter Stamps

disClosure: A Journal of Social Theory

No abstract provided.

Care, Cure, And Control: A Politics Of Dietetics, Rick Dolphijn

disClosure: A Journal of Social Theory

No abstract provided.

Commodifying My Culture: An “Appalachian” Reflects On Her Role In Sustaining A Limited Discourse Of Appalachia, Amanda Fickey

disClosure: A Journal of Social Theory

No abstract provided.

Efficient Activation Of Reconstructed Rat Embryos By Cyclin-Dependent Kinase Inhibitors, Robin L. Webb, Kirk A. Findlay, Michael A. Green, Tina L. Beckett, M. Paul Murphy

Molecular and Cellular Biochemistry Faculty Publications

Background

Over the last decade a number of species, from farm animals to rodents, have been cloned using somatic cell nuclear transfer technology (SCNT). This technique has the potential to revolutionize the way that genetically modified animals are made. In its current state, the process of SCNT is very inefficient (<5% success rate), with several technical and biological hurdles hindering development. Yet, SCNT provides investigators with powerful advantages over other approaches, such as allowing for prescreening for the desired level of transgene expression and eliminating the excess production of undesirable wild-type animals. The rat plays a significant role in biomedical research, but SCNT has been problematic for this species. In this study, we address one aspect of the problem by evaluating methods of activation in artificially constructed rat embryos.

Principal Findings

We demonstrate that treatment with a calcium ionophore (ionomycin) combined with a variety of cyclin-dependent kinase inhibitors is an effective way to activate rat embryos. This is in contrast to methods developed for the mouse embryo, which tolerates much less specific chemical treatments. Methods developed to activate …

Dopamine Neuron Stimulating Actions Of A Gdnf Propeptide, Luke H. Bradley, Josh Fuqua, April Richardson, Jadwiga Turchan-Cholewo, Yi Ai, Kristen A. Kelps, John D. Glass, Xiuquan He, Zhiming Zhang, Richard Grondin, O. Meagan Littrell, Peter Huettl, Francois Pomerleau, Don M. Gash, Greg A. Gerhardt

Neuroscience Faculty Publications

BACKGROUND: Neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), have shown great promise for protection and restoration of damaged or dying dopamine neurons in animal models and in some Parkinson's disease (PD) clinical trials. However, the delivery of neurotrophic factors to the brain is difficult due to their large size and poor bio-distribution. In addition, developing more efficacious trophic factors is hampered by the difficulty of synthesis and structural modification. Small molecules with neurotrophic actions that are easy to synthesize and modify to improve bioavailability are needed.

METHODS AND FINDINGS: Here we present the neurobiological actions of dopamine …

Molecular Evidence For A Functional Ecdysone Signaling System In Brugia Malayi, George Tzertzinis, Ana L. Egaña, Subba Reddy Palli, Marc Robinson-Rechavi, Chris R. Gissendanner, Canhui Liu, Thomas R. Unnasch, Claude V. Maina

Entomology Faculty Publications

BACKGROUND: Filarial nematodes, including Brugia malayi, the causative agent of lymphatic filariasis, undergo molting in both arthropod and mammalian hosts to complete their life cycles. An understanding of how these parasites cross developmental checkpoints may reveal potential targets for intervention. Pharmacological evidence suggests that ecdysteroids play a role in parasitic nematode molting and fertility although their specific function remains unknown. In insects, ecdysone triggers molting through the activation of the ecdysone receptor: a heterodimer of EcR (ecdysone receptor) and USP (Ultraspiracle).

METHODS AND FINDINGS: We report the cloning and characterization of a B. malayi EcR homologue (Bma-EcR). Bma-EcR dimerizes with …

Moving On: Distance Learning At The University Of Kentucky, Randolph Hollingsworth

Undergraduate Education White Papers

This white paper provides the context and historical status of distance learning at the University of Kentucky, offers a global perspective on the potential of an enhanced role for distance learning at the University, and recommends a series of steps to improve the policies, procedures and innovative approaches to distance learning at UK.

Nondestructive Testing Of Defective Astm A 514 Steel On The I ‐ 275 Combs‐Hehl Twin Bridges Over The Ohio River In Campbell County, Kentucky, Theodore Hopwood Ii, Sudhir Palle

Kentucky Transportation Center Research Report

Three defective ASTM A 514 steel splice plates were discovered on the I-275 Combs-Hehl twin bridges over the Ohio River. A follow-on in-depth field inspection of 1,356 A 514 steel plates on the bridges revealed 14 additional defective gusset and splice plates. The A 514 steel was improperly heat-treated resulting in a brittle crack-prone microstructure. Ultrasonic and impact hardness tests were used to identify the defective steel. Subsequently, the defective steel plates were either removed or lapped with additional steel plates.

Kentucky Alfalfa Conference Reflections After 30 Years, Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

This event today marks the 30th consecutive year we have come together for a full day’s conference featuring "Alfalfa - Queen of the Forages" as the theme and focal point. Only one other state (California) in the U.S. has such an annual event.

Evaluation Plan For The Ticketing Aggressive Cars And Trucks (Tact) Program In Kentucky, Eric R. Green

Kentucky Transportation Center Research Report

Kentucky State Police Division of Commercial Vehicle Enforcement in cooperation with Federal Motor Carrier Safety Administration (FMCSA) has started a concentrated education and enforcement campaign in an effort to increase the safety and awareness of drivers around commercial vehicles. The University of Kentucky Transportation Center has evaluated this campaign and reported the effectiveness of this effort. This study is a follow‐up to a 2007 study that focused on two high volume, high crash interstate areas: one in northern Kentucky on I‐75, and one in the Louisville area on I‐65. This study’s focus was moved to I‐75 around Fayette County (Lexington) …

Proteomic Analysis Of Iron Acquisition, Metabolic And Regulatory Responses Of Yersinia Pestis To Iron Starvation, Rembert Pieper, Shih-Ting Huang, Prashanth P. Parmar, David J. Clark, Hamid Alami, Robert D. Fleischmann, Robert D. Perry, Scott N. Peterson

Microbiology, Immunology, and Molecular Genetics Faculty Publications

BACKGROUND: The Gram-negative bacterium Yersinia pestis is the causative agent of the bubonic plague. Efficient iron acquisition systems are critical to the ability of Y. pestis to infect, spread and grow in mammalian hosts, because iron is sequestered and is considered part of the innate host immune defence against invading pathogens. We used a proteomic approach to determine expression changes of iron uptake systems and intracellular consequences of iron deficiency in the Y. pestis strain KIM6+ at two physiologically relevant temperatures (26°C and 37°C).

RESULTS: Differential protein display was performed for three Y. pestis subcellular fractions. Five characterized Y. pestis …

Methods To Impair Hematologic Cancer Progenitor Cells And Compounds Related Thereto, Craig Jordan

Pharmaceutical Sciences Faculty Patents

Primitive or progenitor hematologic cancer cells have been implicated in the early stages and development of leukemia and malignant lymphoproliferative disorders, including acute myelogenous leukemia (AML), chronic myelogenous leukemia (CML) and chronic lymphoid leukemia (CLL). Interleukin-3 receptor alpha chain (IL-3Rα or CD123) is strongly expressed on progenitor hematologic cancer cells, but is virtually undetectable on normal bone marrow cells. The present invention provides methods of impairing progenitor hematologic cancer (e.g., leukemia and lymphomic) cells by selectively targeting cells expressing CD123. These methods are useful in the detection and treatment of leukemias and malignant lymphoproliferative disorders. Also provided are compounds useful …

Notes On Poverty Traps And Appalachia, Steven Durlauf

University of Kentucky Center for Poverty Research Discussion Paper Series

In these notes, I provide some general ideas on how to conceptualize poverty traps and speculate on their applicability to understanding Appalachian poverty. My goal is to stimulate thinking on Appalachia that exploits contemporary perspectives in economics on the sources of persistent poverty and inequality. To do this, I focus on both the theory of poverty traps as well as issues in the econometric assessment of their empirical salience.

A Novel Epimerase That Converts Glcnac-P-P-Undecaprenol To Galnac-P-P-Undecaprenol In Escherichia Coli O157., Jeffrey S. Rush, Cristina Alaimo, Riccardo Robbiani, Michael Wacker, Charles J Waechter

Molecular and Cellular Biochemistry Faculty Publications

Escherichia coli strain O157 produces an O-antigen with the repeating tetrasaccharide unit alpha-D-PerNAc-alpha-l-Fuc-beta-D-Glc-alpha-D-GalNAc, preassembled on undecaprenyl pyrophosphate (Und-P-P). These studies were conducted to determine whether the biosynthesis of the lipid-linked repeating tetrasaccharide was initiated by the formation of GalNAc-P-P-Und by WecA. When membrane fractions from E. coli strains K12, O157, and PR4019, a WecA-overexpressing strain, were incubated with UDP-[3H]GalNAc, neither the enzymatic synthesis of [3H]GlcNAc-P-P-Und nor [3H]GalNAc-P-P-Und was detected. However, when membrane fractions from strain O157 were incubated with UDP-[3H]GlcNAc, two enzymatically labeled products were observed with the chemical and chromatographic properties of [3H]GlcNAc-P-P-Und and [3H]GalNAc-P-P-Und, suggesting that strain O157 …

Neural Network Model For Instruments That Store And Retrieve Sequential Information, Philip W. Landfield, Olivier Thibault

Pharmacology and Nutritional Sciences Faculty Patents

A method and design is provided for distributing and storing sets of temporally ordered information in a systematic and sequential fashion. This method is based on a model of how the brain functions in the distribution and storage of temporally ordered memories, but it can also be applied to the design of new biological, electronic or optical devices. These devices may be used in the testing and development of new therapeutic drugs, in the detection of toxic agents or impaired performance, or in the development of new industrial and consumer devices in which the orderly storage of sequential information is …

First-Time First-Year Full-Tim Students Transitioning From High School To The University Of Kentucky: The Academic Readiness Program, 2009-2010, Randolph Hollingsworth

Undergraduate Education White Papers

This documents the collaborative efforts between the Deans Council, Office of Enrollment Management, Office of Institutional Diversity, Division of Student Affairs and the Office of Undergraduate Education in rolling out the University's response to the state's regulations on college admission and placement for students whose ACT scores are below the regulated cutoff scores. The Academic Readiness Program was developed using the learning outcomes developed by the Directors of Undergraduate Studies in the UK departments of Mathematics and English as well as the expert consultants in the Collaborative Center for Literacy Development. This paper includes the assessment of the 2009-10 Program …

Earnings And Income Volatility In America: Evidence From Matched Cps, James P. Ziliak, Bradley L. Hardy, Christopher Bollinger

University of Kentucky Center for Poverty Research Discussion Paper Series

In this paper we offer new evidence on earnings and income volatility in the United States over the past four decades by using matched data from the March Current Population Survey. We find that between 1973 and 2008 family income volatility rose by 38 percent, primarily as a result of higher volatility of husbands earnings and non means-tested nonlabor income. Rising family income volatility is in evidence across race, education, and family structure, and after declining sharply while young, it is increasing in the latter part of the life cycle among the skilled. The Federal tax and transfer system dampens …