Digital Commons Network^™

Gene Expression Analysis Of A Murine Model With Pulmonary Vascular Remodeling Compared To End-Stage Ipah Lungs, Kayoko Shimodaira, Yoichiro Okubo, Eri Ochiai, Haruo Nakayama, Harutaka Katano, Megumi Wakayama, Minoru Shinozaki, Takao Ishiwatari, Daisuke Sasai, Naobumi Tochigi, Tetsuo Nemoto, Tsutomu Saji, Katsuhiko Kamei, Kazutoshi Shibuya

Microbiology, Immunology, and Molecular Genetics Faculty Publications

BACKGROUND: Idiopathic pulmonary arterial hypertension (IPAH) continues to be one of the most serious intractable diseases that might start with activation of several triggers representing the genetic susceptibility of a patient. To elucidate what essentially contributes to the onset and progression of IPAH, we investigated factors playing an important role in IPAH by searching discrepant or controversial expression patterns between our murine model and those previously published for human IPAH. We employed the mouse model, which induced muscularization of pulmonary artery leading to hypertension by repeated intratracheal injection of Stachybotrys chartarum, a member of nonpathogenic and ubiquitous fungus in our …

Withanolide Compounds As Inhibitors Of Fibrosis And Identification Of Molecular Targets For Anti-Fibrotic Drug Development, Royce Mohan, Paola Bargagna-Mohan

Ophthalmology and Visual Science Faculty Patents

Provided are methods for screening for drugs effective for treating fibrotic conditions. One screening method comprises exposing a cell to a test compound, monitoring the effect of the test compound on the amount or form of a cell molecule, comparing the amount or form of the cell molecule with the result obtained by treatment of the cell with an anti-fibrotic -effective amount of a withanolide compound, and selecting a drug effective for treating a fibrotic disease based on the ability of the test compound to provide the effect obtained by the withanolide compound on the cell molecule. Also provided is …

Cellular Localization Of Aquaporin-1 In The Human And Mouse Trigeminal Systems, Junying Gao, Meiyun Tan, Minxia Gu, Charles Marshall, Jiong Ding, Gang Hu, Ming Xiao

Center of Excellence in Rural Health Faculty Publications

Previous studies reported that a subpopulation of mouse and rat trigeminal neurons express water channel aquaporin-1 (AQP1). In this study we make a comparative investigation of AQP1 localization in the human and mouse trigeminal systems. Immunohistochemistry and immunofluorescence results showed that AQP1 was localized to the cytoplasm and cell membrane of some medium and small-sized trigeminal neurons. Additionally, AQP1 was found in numerous peripheral trigeminal axons of humans and mice. In the central trigeminal root and brain stem, AQP1 was specifically expressed in astrocytes of humans, but was restricted to nerve fibers within the central trigeminal root and spinal trigeminal …

Global Kentucky (Fall 2012), University Of Kentucky International Center

Global Kentucky

No abstract provided.

Southeastern Law Librarian Fall 2012, Seaall

Newsletters

No abstract provided.

Can Consortial Reference Partners Answer Your Local Users’ Library Questions?, Bradley Wade Bishop

Information Science Faculty Publications

The purpose of this article is to explore location-based questions as a weakness of virtual reference consortia and discuss how to mitigate related issues. Content analysis of how both local and non-local academic librarians responded to location-based questions provides insight into considerations academic libraries must make when participating in a virtual reference consortia. Unobtrusive testing analyzed the local knowledge assumption that non-local librarians have difficulty answering questions about libraries beyond their own. The results from these two methods indicate academic librarians have some difficulties providing responses to library location-based questions and a discussion on overcoming this weakness is included.

The Great Recession And The Rhetorical Canons Of Law And Economics, Michael D. Murray

Law Faculty Scholarly Articles

The Great Recession of 2008 and onward has drawn attention to the American economic and financial system and has cast a critical spotlight on the theories, policies, and assumptions of the modern, neoclassical school of law and economics-often labeled the "Chicago School"-because this school of legal economic thought has had great influence on the American economy and financial system. The Chicago School's positions on deregulation and the limitation or elimination of oversight and government restraints on stock markets, derivative markets, and other financial practices are the result of decades of neoclassical economic assumptions regarding the efficiency of unregulated markets, the …

Characterization Of Secretory Sphingomyelinase Activity, Lipoprotein Sphingolipid Content And Ldl Aggregation In Ldlr-/- Mice Fed On A High-Fat Diet, Gergana M. Deevska, Manjula Sunkara, Andrew J. Morris, Mariana N. Nikolova‑Karakashian

Physiology Faculty Publications

The propensity of LDLs (low-density lipoproteins) for aggregation and/or oxidation has been linked to their sphingolipid content, specifically the levels of SM (sphingomyelin) and ceramide. To investigate this association in vivo, ldlr (LDL receptor)-null mice (ldlr-/-) were fed on a modified (atherogenic) diet containing saturated fats and cholesterol. The diet led to significantly elevated SM content in all serum lipoproteins. In contrast, ceramide increased only in the LDL particles. MS-based analyses of the lipid acyl chain composition revealed a marked elevation in C16:0 fatty acid in SM and ceramide, consistent with the prevalence of palmitic acid in the modified diet. …

Doxycycline Does Not Influence Established Abdominal Aortic Aneurysms In Angiotensin Ii-Infused Mice, Xiaojie Xie, Hong Lu, Jessica J. Moorleghen, Deborah A. Howatt, Debra L. Rateri, Lisa A. Cassis, Alan Daugherty

Saha Cardiovascular Research Center Faculty Publications

Background: There is no proven medical approach to attenuating expansion and rupture of abdominal aortic aneurysms (AAAs). One approach that is currently being investigated is the use of doxycycline. Despite being primarily used as an antimicrobial drug, doxycycline has been proposed to function in reducing AAA expansion. Doxycycline is effective in reducing the formation in the most commonly used mouse models of AAAs when administered prior to the initiation of the disease. The purpose of the current study was to determine the effects of doxycycline on established AAAs when it was administered at a dose that produces therapeutic serum …

Latexin Is Down-Regulated In Hematopoietic Malignancies And Restoration Of Expression Inhibits Lymphoma Growth, Yi Liu, Dianna Howard, Kyle Rector, Carol Swiderski, Jason Brandon, Lawrence Schook, Jayesh Mehta, J. Scott Bryson, Subbarao Bondada, Ying Liang

Internal Medicine Faculty Publications

Latexin is a negative regulator of hematopoietic stem cell number in mice. Its dysregulated expression in other tumors led us to hypothesize that latexin may have tumor suppressor properties in hematological malignancies. We found that latexin was down-regulated in a variety of leukemia and lymphoma cell lines as well as in CD34+ cells from the blood and marrow of patients with these malignancies. 5-aza-2'-deoxycytodine treatment and bisulfite sequencing revealed hypermethylation of latexin promoter in tumor cells. Retrovirus-mediated latexin overexpression in A20 mouse lymphoma cells inhibited their in vitro growth by 16 fold and in vivo tumor volume by 2 fold. …

Molecular Characterization Of The Viab Locus Encoding The Biosynthetic Machinery For Vi Capsule Formation In Salmonella Typhi, Michael Wetter, David Goulding, Derek Pickard, Michael Kowarik, Charles J. Waechter, Gordon Dougan, Michael Wacker

Molecular and Cellular Biochemistry Faculty Publications

The Vi capsular polysaccharide (CPS) of Salmonella enterica serovar Typhi, the cause of human typhoid, is important for infectivity and virulence. The Vi biosynthetic machinery is encoded within the viaB locus composed of 10 genes involved in regulation of expression (tviA), polymer synthesis (tviB-tviE), and cell surface localization of the CPS (vexA-vexE). We cloned the viaB locus from S. Typhi and transposon insertion mutants of individual viaB genes were characterized in Escherichia coli DH5α. Phenotype analysis of viaB mutants revealed that tviB, tviC, tviD and tviE are involved in Vi polymer synthesis. Furthermore, expression of tviB-tviE in E. coli DH5α …

Wpa: An Important Chapter In U.S. And Kentucky History, Mary Mclaren, Jennifer Bartlett, Angelia Pulley

Library Presentations

Guidebooks, art, archival inventories, drama, public buildings, and road construction come to mind as unique chapters of the Works Progress Administration (WPA) program during depression-era U.S. history. This session will explain what it means for the University of Kentucky Libraries to be designated as an ASERL Center for Excellence for WPA publications. It will highlight key WPA publications with emphasis on public access, both print and electronic, as well as UK’s efforts to promote the collection.

Perlecan Domain V Induces Vegf Secretion In Brain Endothelial Cells Through Integrin Α5Β1 And Erk-Dependent Signaling Pathways, Douglas N. Clarke, Abraham Al Ahmad, Boyeon Lee, Christi Parham, Lisa Auckland, Andrezj Fertala, Michael Kahle, Courtney S. Shaw, Jill Roberts, Gregory J. Bix

Sanders-Brown Center on Aging Faculty Publications

Perlecan Domain V (DV) promotes brain angiogenesis by inducing VEGF release from brain endothelial cells (BECs) following stroke. In this study, we define the specific mechanism of DV interaction with the α(5)β(1) integrin, identify the downstream signal transduction pathway, and further investigate the functional significance of resultant VEGF release. Interestingly, we found that the LG3 portion of DV, which has been suggested to possess most of DV's angio-modulatory activity outside of the brain, binds poorly to α(5)β(1) and induces less BEC proliferation compared to full length DV. Additionally, we implicate DV's DGR sequence as an important element for the interaction …

Cultural Institutions In The Digital Age: Finding Common Ground, David S. Ferriero

Putting America Back to Work during the Great Depression: Preserving and Improving Access to the Works Progress Administration Records for the Future

No abstract provided.

Kentucky Water Resources Research Institute Center Of Excellence For Watershed Management Watershed Summit Summary Report, Kentucky Water Resources Research Institute, University Of Kentucky

KWRRI Research Reports

No abstract provided.

Characterizing Physical Properties Of Gas-Phase Biofilter Media, Guilherme Del Nero Maia, G. Tatiana Sales, George B. Day V, Richard S. Gates, Joseph L. Taraba

Biosystems and Agricultural Engineering Faculty Publications

Gas-phase biofiltration is an effective technology for reduction of odors and trace-gas contaminants. Significant contributions to the technical literature regarding the characterization of biofilter media have been generated in the past two decades. Nevertheless, the information produced has not been systematically organized. The objective of this study is to demonstrate and document methods for physical characterization of gas-phase compost biofilters (GPCB). The inclusion of moisture content, compaction, and particle size effects in the determination of media bulk density and porosity, field capacity, drying rate analysis, water sorption isotherms, and resistance to airflow is demonstrated. Results indicated that: (1) higher moisture …

An Empirical Examination Of The Associations Between Social Tags And Web Queries, Kwan Yi, Chan Yun Yoo

Journalism and Media Faculty Publications

Introduction. We aim to discover the associations between social tags for a Web page and Web queries that would retrieve the same Webpage in three major search engines.

Method. 4,827 query terms were submitted to the three major search engines to acquire search engine results pages. A series of Perl scripts were written to read search engine results pages and to identify, analyse, and extract organic links

Analysis. Web pages from the organic links in search engine results pages were examined to see whether and how they had been tagged in Delicious. Only the Webpages tagged by …

Graffiti, Poetry, Dance: How Public Library Art Programs Affect Teens Part 1: Introduction & Literature Review, Shannon Crawford Barniskis

Communication Faculty Publications

This two-part article addresses the outcomes of art programs in public libraries by examining the literature, and by asking teens how these programs can affect their civic engagement. The literature review synthesizes previous research on art, libraries, teens, and civic engagement, and positions this case study in relation to the theoretical constructs of adult researchers.

The case study generates a grounded theory of the teen experience of art programs and its correlating shifts in civic engagement. Fourteen teens joined in six weekly arts programs, responded to surveys, and participated in interviews on art, libraries, various measures of civic engagement, and …

Graffiti, Poetry, Dance: How Public Library Art Programs Affect Teens Part 2: The Research Study And Its Practical Implications, Shannon Crawford Barniskis

Communication Faculty Publications

Part 1 of this article synthesized the literature on civic engagement, art, libraries, and teen services to demonstrate how public libraries can transform the lives of their patrons and the communities they serve. This case study responds to the top-down view of this topic generated by adult researchers by creating a bottom-up, teen-validated grounded theory. In my thirteen years as a teen librarian, it seemed that something special was occurring in the art programs I hosted or observed in public libraries. Teens were not simply creating steampunk sculptures, they were also appeared to be connecting with one another in interesting …

Atovaquone Ameliorate Gastrointestinal Toxoplasmosis Complications In A Pregnancy Model, Helieh S. Oz, Thomas Tobin

Physiology Faculty Publications

Background: Toxoplasma is an important source of foodborne hospitalization with no safe and effective therapy against chronic or congenital Toxopalsmosis. Atovaquone is a drug of choice but not approved for use in congenital Toxoplasmosis. We hypothesized atovaquone to be safe and effective against feto-maternal Toxoplasmosis.

Material/Methods: Programmed pregnant mice were i.p. infected with 50–2400 Tachyzoites from Type II strain (clone PTG). Dams were treated daily with atovaquone or sham and monitored for pain, and complications.

Results: Dams developed pain related abdominal hypersensitivity (allodynia) to mechanical stimuli in a Tachyzoites dose dependent manner. Infected dams were anemic and exhibited ascities and …

Role Of Sequence And Structure Of The Hendra Fusion Protein Fusion Peptide In Membrane Fusion, Everett Clinton Smith, Sonia M. Gregory, Lukas K. Tamm, Trevor P. Creamer, Rebecca Ellis Dutch

Molecular and Cellular Biochemistry Faculty Publications

Viral fusion proteins are intriguing molecular machines that undergo drastic conformational changes to facilitate virus-cell membrane fusion. During fusion a hydrophobic region of the protein, termed the fusion peptide (FP), is inserted into the target host cell membrane, with subsequent conformational changes culminating in membrane merger. Class I fusion proteins contain FPs between 20 and 30 amino acids in length that are highly conserved within viral families but not between. To examine the sequence dependence of the Hendra virus (HeV) fusion (F) protein FP, the first eight amino acids were mutated first as double, then single, alanine mutants. Mutation of …

Reflective Journals Of Students Taking A Positive Youth-Development Course In A University Context In Hong Kong, Daniel T. L. Shek, Florence K. Y. Wu

Pediatrics Faculty Publications

To promote the holistic development of university students, a course entitled "Tomorrow's Leaders" was developed and offered at The Hong Kong Polytechnic University. Based on a case study approach, reflective journals of five outstanding students of the course are presented and analyzed (i.e., thick description), with several themes emerging from the reflection. First, the students liked the course, and they identified many positive attributes. Second, the students appreciated the instructors. Third, the students viewed that the course contributed to different aspects of their development. Fourth, some areas of improvements were proposed. In conjunction with other evaluation mechanisms, the present findings …

Retention Improvement: Reflecting On Current And Future Actions To Improve Retention - A Brief Written By Jason Pieratt On Behalf Of The Division Of Undergraduate Education, Randolph Hollingsworth

Undergraduate Education Faculty Presentations

Jason Pieratt, an enrollment and retention analyst hired by the Division of Undergraduate Education for the purpose of developing this brief, interviewed in early August 2012 selected individuals from across the diverse communities of the University of Kentucky staff, students and faculty. The following strategies were identified and developed.

University-wide:

• “Student Success is My Main Job” Mindset for All Employees (particularly Main Campus)
• Weave Post-UK Success into Everything from Recruiting to Graduation
• Capitalize on “Small” Settings that Already Exist at UK
• Student Services Building One-Stop Shop
• Retention-Focused Office under the Provost
• Academic and Social Gathering Places throughout Campus
• Faculty Involvement …

Coronary Artery Remodeling In A Model Of Left Ventricular Pressure Overload Is Influenced By Platelets And Inflammatory Cells, Fanmuyi Yang, Anping Dong, Paul Mueller, Jessica Caicedo, Alyssa Moore Sutton, Juliana Odetunde, Cordelia J. Barrick, Yuri M. Klyachkin, Ahmed Abdel-Latif, Susan S. Smyth

Internal Medicine Faculty Publications

Left ventricular hypertrophy (LVH) is usually accompanied by intensive interstitial and perivascular fibrosis, which may contribute to arrhythmogenic sudden cardiac death. The mechanisms underlying the development of cardiac fibrosis are incompletely understood. To investigate the role of perivascular inflammation in coronary artery remodeling and cardiac fibrosis during hypertrophic ventricular remodeling, we used a well-established mouse model of LVH (transverse aortic constriction [TAC]). Three days after pressure overload, macrophages and T lymphocytes accumulated around and along left coronary arteries in association with luminal platelet deposition. Consistent with these histological findings, cardiac expression of IL-10 was upregulated and in the systemic circulation, …

Women, Sustainability, And Development, Alison Huddleston

Kaleidoscope

No abstract provided.

An Ecological Analysis Of Same-Sex Domestic Violence Between Gay Men, Ericka Barbour

Kaleidoscope

No abstract provided.

Novel Role Of A Cypovirus In Polydnavirus-Parasitoid-Host Relationship, Philip Houtz

Kaleidoscope

No abstract provided.

Childhood Depression And Obesity: Role Of The Hypothalamic-Pituitary-Adrenal (Hpa) Axis Activity, Joanne Tyler

Kaleidoscope

This study examined relations between child depression and eating behavior and weight problems, including the role ofbaseline cortisol and cortisol reactivity in these relations. Data were drawn from a larger study, and included 50 children between the ages of6 and 12. Children reported on their depressive symptoms, and children and parents reported on child engagement in external eating, under-eating, over-eating, parental pressure to eat, enjoyment of food, and food and satiety responsiveness. For children exhibiting hypercortisolism, depression was associated with decreased over-eating and food responsiveness. Hypercortisolism was especially important for girls and children later in pubertal development. The reduced appetite …

The Uyghurs Of China: A Struggle Of Past, Present, And Future, Clinton Parker

Kaleidoscope

No abstract provided.

Biocomaptibility Analysis And Cancer Therapy Applications Of Core-Shell Composite Magnetic Nanoparticles, Anastasia Kruse, Robert J. Wydra, J. Zach Hilt, Kimberly W. Anderson

Kaleidoscope

No abstract provided.