Digital Commons Network^™

Immobilizing Mutation In An Unconventional Myosin15a Affects Not Only The Structure Of Mechanosensory Stereocilia In The Inner Ear Hair Cells But Also Their Ionic Conductances, Diana Syam

Theses and Dissertations--Medical Sciences

In the inner and outer hair cells (OHCs) of the inner ear, an unconventional myosin 15a localizes at the tips of mechanosensory stereocilia and plays an important role in forming and maintaining their normal structure. A missense mutation makes the motor domain of myosin 15a dysfunctional and is responsible for the congenital deafness DFNB3 in humans and deafness and vestibular defects in Shaker-2 (Sh2) mouse model. All hair cells of homozygous Shaker-2 mice (Myo15^sh2/sh2) have abnormally short stereocilia, but, only stereocilia of Myo15^sh2/sh2OHCs start to degenerate after the first few days of postnatal development …

Contaminated Chi-Square Modeling And Its Application In Microarray Data Analysis, Feng Zhou

Theses and Dissertations--Statistics

Mixture modeling has numerous applications. One particular interest is microarray data analysis. My dissertation research is focused on the Contaminated Chi-Square (CCS) Modeling and its application in microarray. A moment-based method and two likelihood-based methods including Modified Likelihood Ratio Test (MLRT) and Expectation-Maximization (EM) Test are developed for testing the omnibus null hypothesis of no contamination of a central chi-square distribution by a non-central Chi-Square distribution. When the omnibus null hypothesis is rejected, we further developed the moment-based test and the EM test for testing an extra component to the Contaminated Chi-Square (CCS+EC) Model. The moment-based approach is easy and …

Quantification Of Factors Governing Drug Release Kinetics From Nanoparticles: A Combined Experimental And Mechanistic Modeling Approach, Kyle Daniel Fugit

Theses and Dissertations--Pharmacy

Advancements in nanoparticle drug delivery of anticancer agents require mathematical models capable of predicting in vivo formulation performance from in vitro characterization studies. Such models must identify and incorporate the physicochemical properties of the therapeutic agent and nanoparticle driving in vivo drug release. This work identifies these factors for two nanoparticle formulations of anticancer agents using an approach which develops mechanistic mathematical models in conjunction with experimental studies.

A non-sink ultrafiltration method was developed to monitor liposomal release kinetics of the anticancer agent topotecan. Mathematical modeling allowed simultaneous determination of drug permeability and interfacial binding to the bilayer from release …

Mechanisms Of Cyclooxygenase-2-Dependent Human Aortic Smooth Muscle Cell Phenotypic Modulation, Oreoluwa O. Adedoyin

Theses and Dissertations--Pharmacy

Abdominal aortic aneurysm (AAA) is a disease of the aorta characterized by pathological remodeling and progressive weakening of the vessel resulting in the increased risk of rupture and sudden death. In a mouse model of the disease induced by chronic Angiotensin II (AngII) infusion, progression of AAAs is associated with reduced differentiation of smooth muscle cells (SMCs) at the site of lesion development. In the mouse model, the effectiveness of cyclooxygenase-2 (COX-2) inhibition for attenuating AAA progression is associated with maintenance of a differentiated SMC phenotype. However, the safety of COX-2 inhibitors is currently in question due to the increased …

Flavonoids With Novel Nicotinic Activity As Potential Pharmacotherapies To Treat Ethanol-Induced Neurotoxicity, Joseph A. Lutz

Theses and Dissertations--Pharmacy

Ethanol causes neurotoxicity via several mechanisms at different points in the cycle of dependence, including neuroinflammation and oxidative stress during ethanol exposure as well as excitotoxicity during ethanol withdrawal. The primary therapeutic implication is that ethanol-induced neurotoxicity requires multifunctional pharmacotherapies which reduce all mechanisms. Using an innovative pharmacological high throughput screening method on a large plant extract library we discovered flavonoids with alpha7 nicotinic acetylcholine receptor (nAChR) activity. In addition to their well-known anti-inflammatory and antioxidant properties, this novel activity means they can potentially reduce excitotoxicity and therefore makes them ideal for inhibition of ethanol-induced neurotoxicity. Rhamnetin, the candidate compound, …

Phosphatidylinositol 3-Kinase (Pi3k) As A Therapeutic Target In Nsclc, Christopher W. Stamatkin

Theses and Dissertations--Pharmacy

Deregulated activation of phosphatidylinositol 3-kinase (PI3K) pathway is central to many human malignancies. The functions of this pathway are critical for normal cell metabolism, proliferation, and survival. In lung cancers, the PI3K pathway activity is often aberrantly driven by multiple mutations, including EGFR, KRAS, and PIK3CA. Molecules targeting the PI3K pathway are intensely investigated as potential anti-cancer agents. Although inhibitors of the pathway are currently in clinical trials, rational and targeted use of these compounds, alone or in combination, requires an understanding of isoform-specific activity in context. We sought to identify class IA PI3K enzyme (p110a/PIK3CA, p110b/PIK3CB, p110d/PIK3CD) activities using …

How Do Consumers Use Social Shopping Websites? The Impact Of Social Endorsements, Pei Xu

Theses and Dissertations--Business Administration

Social endorsements are user-generated endorsements of products or services, such as “likes” and personal collections, in an online social platform. We examine the effect of prior social endorsements on subsequent users’ tendency to endorse or examine a product in a social shopping context, where a social platform connect consumers and enable a collaborative shopping experience. This research consists of two parts. In part I, we identify two ways prior social endorsements can affect subsequent user behavior: as a crowd endorsement, which is an aggregate number of endorsements a product receives for anyone who comes across the product, and as …

Multiscale Modeling Of The Mine Ventilation System And Flow Through The Gob, William Chad Wedding

Theses and Dissertations--Mining Engineering

The following dissertation introduces the hazard of methane buildup in the gob zone, a caved region behind a retreating longwall face. This region serves as a reservoir for methane that can bleed into the mine workings. As this methane mixes with air delivered to the longwall panel, explosive concentrations of methane will be reached.

Computational fluid dynamics (CFD) is one of the many approaches to study the gob environment. Several studies in the past have researched this topic and a general approach has been developed that addresses much of the complexity of the problem. The topic of research herein presents …

Development Of Industry Oriented Cfd Code For Analysis / Design Of Face Ventilation Systems, Todor P. Petrov

Theses and Dissertations--Mining Engineering

Two of the main safety and health issues recognized during deep cut coal mining are methane and dust hazards. Advances in continuous miner technology have improved safety and productivity. However, these advances have created some environmental problems, notably more dust and methane being generated at the face during coal extraction.

Results of studies performed in the last three decades concerning the face ventilation for deep cut mining showed very complicated airflow behavior. The specifics of flow patterns developed by the face ventilation systems presents significant challenge for analytical description even for equipment-free entry. Fortunately, there are methods, such as numerical …

Fragmentation Analysis In The Dynamic Stress Wave Collision Regions In Bench Blasting, Catherine E. Johnson

Theses and Dissertations--Mining Engineering

The first step in many mining operations is blasting, and the purpose of blasting is to fragment the rock mass in the most efficient way for that mine site and the material end use. Over time, new developments to any industry occur, and design and implementation of traditional techniques have to change as a consequence. Possibly the greatest improvement in blasting in recent years is that of electronic detonators. The improvements related to safety and increased fragmentation have been invaluable. There has been ongoing debate within the explosives industry regarding two possible theories for this. Shorter timing delays that allow …

Numerical Analysis Of Droplet Formation And Transport Of A Highly Viscous Liquid, Peiding Wang

Theses and Dissertations--Mechanical and Aerospace Engineering

Drop-on-demand (DOD) inkjet print-head has a major share of the market due to simplicity and feasibility of miniature system. The efficiency of droplet generation from DOD print-head is a result of several factors, include viscosity, surface tension, nozzle size, density, driving waveform (wave shape, frequency, and amplitude), etc. Key roles in the formation and behavior of liquid jets and drops combine three dimensionless groups: Reynolds number, Weber number and Ohnesorge number. These dimensionless groups provide some bounds to the “printability” of the liquid. Adequate understanding of these parameters is essential to improve the quality of droplets and provide guidelines for …

Multidimensional Modeling Of Pyrolysis Gas Transport Inside Orthotropic Charring Ablators, Haoyue Weng

Theses and Dissertations--Mechanical and Aerospace Engineering

During hypersonic atmospheric entry, spacecraft are exposed to enormous aerodynamic heat. To prevent the payload from overheating, charring ablative materials are favored to be applied as the heat shield at the exposing surface of the vehicle. Accurate modeling not only prevents mission failures, but also helps reduce cost. Existing models were mostly limited to one-dimensional and discrepancies were shown against measured experiments and flight-data. To help improve the models and analyze the charring ablation problems, a multidimensional material response module is developed, based on a finite volume method framework. The developed computer program is verified through a series of test-cases, …

Using Video Based Instruction To Teach Art To Students With Autism Spectrum Disorder, Anthony W. Woodruff

Theses and Dissertations--Art and Visual Studies

Students with Autism Spectrum Disorders often do not respond to common pedagogical strategies and traditional in vivo teaching approaches. From my observations, students diagnosed with Autism Spectrum Disorders learn best when material is presented through technology. Based on the information found in the literature review and personal observations from working with students with special needs, when students with Autism Spectrum Disorders receive video based instruction by watching recorded art lessons, they may learn to develop artistic skills and retain more art content knowledge with greater success than through traditional teaching methods. The purpose of this research study is to test …

Looking To The Future, Selling The Past: Churchill Weavers Marketing Strategies In The 1950s, Cassandra White-Fredette

Theses and Dissertations--Art and Visual Studies

This thesis explores the Churchill Weavers stereocards housed at the Kentucky Historical Society and Berea College based on visual analysis. By examining the stereocards as advertisements and comparing them to a series of short films created by the company, I will discuss how the Churchill Weavers created a brand that emphasized both an image of traditional American rural production and modern urban consumption. I will further discuss how the marketing strategies used by the Churchill Weavers exemplify a larger trend in American advertising in the years following World War Two.

Edward Steichen And Hollywood Glamour, Alisa Reynolds

Theses and Dissertations--Art and Visual Studies

As a word, glamour is hard to define, but is instantly recognizable. Its association with Hollywood movie stars fully emerged in the 1930s in the close-up celebrity portraits by photographers like George Hurrell. The aesthetic properties in these images that help create glamour are characterized by the Modernist style, known for sharp focus, high contrast, seductive poses, and the close-up (tight framing). My essay will explore the origins of the visual aesthetics of glamour, arguing that their roots can be found in the still life photographs of the 1910s, produced by fine art photographers such as Edward Steichen. This essay …

Nucleoside Reverse Transcriptase Inhibitors Are Anti-Inflammatory And Target Dry Age-Related Macular Degeneration, Benjamin J. Fowler

Theses and Dissertations--Physiology

Age-related macular degeneration (AMD) is a principal cause of blindness in the United States and other industrialized nations. An estimated 10 million Americans are afflicted with AMD, which is comparable in scope to the 12 million living with cancer, or the 5 million with Alzheimer’s disease. The prevalence of AMD steadily increases with age, affecting 2% of the population at age 40, and one in four people by age 80. For reasons that are not fully understood, AMD is more common in lightly-pigmented and female populations. Treatment of AMD is largely an unmet need: There are no FDA approved therapies …

Trpa1 Channels In Cochlear Supporting Cells Regulate Hearing Sensitivity After Noise Exposure, Alejandra C. Velez-Ortega

Theses and Dissertations--Physiology

TRPA1 channels are sensors for noxious stimuli in a subset of nociceptive neurons. TRPA1 channels are also expressed in cells of the mammalian inner ear, but their function in this tissue remains unknown given that Trpa1^–/– mice exhibit normal hearing, balance and sensory mechanotransduction. Here we show that non-sensory (supporting) cells of the hearing organ in the cochlea detect tissue damage via the activation of TRPA1 channels and subsequently modulate cochlear amplification through active cellshape changes.

We found that cochlear supporting cells of wild type but not Trpa1^–/– mice generate inward currents and robust long-lasting Ca²⁺ responses …

Board Governance Of World-Class Annual Sport Championships: Learning From Kentucky To Create Tourism Opportunities In The Bahamas, Elvis V. Burrows

Theses and Dissertations--Retailing and Tourism Management

The economy of The Bahamas relies heavily on leisure visitor tourism (cruise ships, resorts, holiday trips) which leaves the country vulnerable to changes in visitor travel habits. Because the country is so reliant on investments in resorts, casinos and cruise ships, it leaves a wide capacity of other touristic opportunities. One opportunity is sports tourism. The purpose of this study is to analyze the strategic board capability of the Rolex Kentucky Three Day Event’s (RK3DE) board governance using the Ferkins and Shilbury (2012) model as the theoretical construct and make recommendations regarding the application of these successful aspects to the …

Brand Analyses Of Global Brands Versus Local Brand In Indian Apparel Consumer Market, Aniket Sengupta

Theses and Dissertations--Retailing and Tourism Management

The purpose of this study was to conduct brand analyses on global brands in comparison to local retailers in India taking into account the regional differences and Indian consumers’ affinity towards global brands. The study has derived its conceptual framework from previous work done by Lee, Knight, and Kim (2008) and Bhardwaj, Kumar and Youn-Kyung (2010) with some added attributes. Quantitative data included a sample of 194 subjects where the sampling was conducted randomly as well as the involvement of convenient method to analyze the brands in real-world scenario. The survey involved the questionnaire which was utilized in previous researches …

Evaluation Of Pcr-Based Methods For Rapid, Accurate Detection And Monitoring Of Verticillium Dahliae In Woody Hosts By Real-Time Polymerase Chain Reaction, Baker Diwan Getheeth Aljawasim

Theses and Dissertations--Plant Pathology

Verticillium wilt, caused by Verticillium dahliae Kleb, is one of the most economically important diseases of woody hosts such as ash (Fraxinus spp.), sugar maple (Acer saccharum), and redbud (Cercis canadensis). The causal agent has a broad host range, including not only woody hosts but also important vegetable and field crops, and it is distributed worldwide. Diagnosis of V. dahliae in infected woody hosts is often based on the occurrence of vascular discoloration and time-consuming isolation. However, not all woody hosts exhibit vascular discoloration symptoms, and not all vascular discoloration symptoms are due to infection …

The Roles Of Orthopaedic Pathology And Genetic Determinants In Equine Cervical Stenotic Myelopathy, Jennifer Gail Janes

Theses and Dissertations--Veterinary Science

Cervical stenotic myelopathy (CSM) is an important musculoskeletal and neurologic disease of the horse. Clinical disease occurs due to malformations of the vertebrae in the neck causing stenosis of the cervical vertebral canal and subsequent spinal cord compression. The disease is multifactorial in nature, therefore a clearer understanding of the etiology and pathogenesis of CSM will allow for improved management and therapeutic practices. This thesis examines issues of equine CSM diagnosis, skeletal tissue pathology, and inherited genetic determinants utilizing advances in biomedical imaging technologies and equine genomics. Magnetic resonance imaging (MRI) data provided a more complete assessment of the cervical …

Evaluation Of The Susceptibility And Humoral Immune Response Of Foals To Rhodococcus Equi Infection, Macarena G. Sanz

Theses and Dissertations--Veterinary Science

While Rhodococcus equi (R. equi) remains the most common cause of subacute or chronic granulomatous bronchopneumonia in foals, development of a relevant model to study this bacterium has proven difficult. As a result, the reasons for the underlying foal’s susceptibility to this disease are not well understood. Furthermore, data regarding the immune response of foals to R. equi infection remains controversial. We hypothesized that foals are susceptible to R. equi early in life and that this susceptibility decreases with age. Also, we hypothesized that specific subclasses of IgG antibodies to the virulence-associated protein of R. equi, VapA, …

Anti-Müllerian Hormone In Stallions And Mares: Physiological Variations, Clinical Applications, And Molecular Aspects, Anthony N.J. Claes

Theses and Dissertations--Veterinary Science

Anti-Müllerian hormone (AMH) is a homodimeric glycoprotein that is best known for its role in regression of the Müllerian duct in the male fetus. Accumulating evidence indicates that AMH also has an important role during different physiological processes after birth. In contrast to other species, relatively little is known about AMH in the horse. In chapter one, developmental and seasonal changes in serum AMH concentrations in male horses were determined, and the use of AMH for determination of retained cryptorchid testes was established. In chapter two, the interrelationship between plasma AMH concentrations, antral follicle counts (AFC), and age in mares …

Attitudes Toward Assisted Reproductive Technology: The Effects Of Gender, Relationship Status, Age, And Sexual Orientation, Brigitte A. Dooley

Theses and Dissertations--Family Sciences

Reproductive technology has extended procreative options to infertile, subfertile, unpartnered, and same-sex-partnered individuals, but this technology is sometimes used in circumstances that may be deemed unreasonable or inappropriate by some people. The purpose of this study was to assess the effects of five contextual variables—gender, relationship status, age, and sexual orientation of the individual or couple seeking reproductive assistance, as well as the source of gametes—on attitudes toward the procurement of reproductive services. A multiple-segment factorial vignette was administered to a sample of 257 reproductive-aged respondents. Results indicate that ART is generally viewed as an acceptable procedure by reproductive aged …

Social And Psychological Factors Related To The Career Exploration Process Of Young Adults, Joanne N. Hellmann

Theses and Dissertations--Family Sciences

This study examined social and psychological factors influencing the career exploration process of young adults. The predictor variables of this study were parental attachment, peer attachment, and self-efficacy; the outcome variables were environmental/occupational exploration, self-exploration, and career indecision. Data for this study were collected using various measures that were compiled into one survey hosted on Qualtrics. Results indicated that secure maternal attachment predicted secure peer attachment and high self-efficacy in young adults. Greater peer attachment was negatively correlated with environmental exploration. A higher level of self-efficacy was positively correlated with environmental exploration and negatively correlated with career indecision. These results …

Factors In Breastfeeding Initiation Among Central Kentucky Wic And Non-Wic Participants, Lila Nicole Farnsworth

Theses and Dissertations--Nutrition and Food Systems

Vital statistics data were statistically analyzed to determine who is breastfeeding in central Kentucky and if factors differ between those in a WIC and non-WIC population. The sample consisted of 479 postpartum women aged 18-44 in central Kentucky. Participants in the study were analyzed as a whole, then divided by their participation in the WIC program (n=304) or non-participation in the WIC program (n=175). Variables recorded in the vital statistics form were analyzed. These variables included age, education level, marital status, and ethnicity of the mother, household income status, and gender, gestational age, and birth weight of the infant. In …

Promoting Healthy, Home-Cooked Meals: Formative Research For A Social Marketing Program Targeting Low-Income Mothers, Jean M. Najor

Theses and Dissertations--Nutrition and Food Systems

The purpose of this study is to gain a deeper understanding of the dynamic of perceived benefits and barriers associated with cooking meals at home. The findings will be used to design a social marketing campaign promoting healthy home-cooked meals. Participants included 64 SNAP-eligible mothers throughout the state of Kentucky aged 21-49 years with young children. Eight focus groups were conducted in four metro and four non-metro counties. A mixed methods approach was used to examine behaviors such as where families purchase foods, the types of foods purchased, family cooking skills and habits, and family time management. Results from this …

Primary Stroke Prevention: 3-Hydroxy-3-Methyl-Glutaryl-Coenzyme A (Hmg-Coa) Reductase Inhibitor (Statin) Use In The Diabetic Patient, Amy L. Hatcher

DNP Projects

Purpose: The primary objective for this retrospective chart review was to evaluate provider adherence to the 2011 AHA/ASA Primary Stroke Prevention guideline of prescribing a statin to all diabetic patients for primary prevention of stroke, regardless of dyslipidemia. A second aim of this study is to identify provider facilitators and barriers to prescribing a statin therapy for primary prevention.

Methods: Using a retrospective study design, a random sample of 100 medical records of diabetic patients presenting to a university women’s health clinic within the previous year were reviewed for statin use and rationale.

Results: Of the 100 diabetic patients sampled, …

Letting Teams Walk: Exploring The Economic Impact Of Professional Sports Franchises Leaving Cities, Jesse Stephenson

MPA/MPP/MPFM Capstone Projects

Cities often use public funding to help construct and renovate the facilities of large, private sports franchises. As the major sports leagues grow in popularity and revenue, cities have had to offer increasingly competitive incentive packages to owners in order to keep their team from relocating to more profitable markets or cities willing to issue public funding for new facilities. Much literature has been conducted on the economic impact of sports teams with a general consensus that there is usually no positive economic effect for cities that serve as homes for these teams from the largest leagues (NFL, MLB, and …

Spending For Affordable Housing: How Lexington-Fayette Urban County, Ky Compares To Similar Cities In The U.S., Chenglin Zhao

MPA/MPP/MPFM Capstone Projects

Objectives: The shortage of affordable housing in Lexington-Fayette urban county has become an important policy issue. The analysis in this study is designed to find out does Lexington-Fayette Urban County do better or worse in financing affordable housing for low-income residents.

Method: By comparing Lexington-Fayette Urban County with 11 similar cities in U.S., there may reveal options for the Lexington-Fayette Urban County government to improve housing supply financing assistance services, find new funding sources for affordable housing and ensure more low-income residents can find affordable housing.

Key finding: Nonprofit housing operations play meaningful roles in addressing the supply of affordable …