Digital Commons Network^™

Ex Vivo Single-Molecule Analysis Of Ryanodine Receptor 2 Assembly In Cardiac And Neuronal Tissue, Qianye Yin, Christopher I. Richards

Chemistry Faculty Publications

Understanding receptor assembly is critical for elucidating the mechanisms underlying their function and regulation in physiological processes. While traditional in vitro single-molecule studies rely on isolating proteins from heterologous expression systems, they often fail to capture the in vivo physiological complexity involved in the organization and assembly of cell surface proteins. This protocol employs Total Internal Reflection Fluorescence Microscopy (TIRFM) to study GFP-tagged Ryanodine Receptor 2 (RyR2) molecules encapsulated within nanoscale vesicles. These vesicles, generated from organs rapidly extracted from the animal, effectively provide a snapshot of the receptor's assembly state at the time of extraction, enabling detailed analysis of …

Incarceration Reimagined: A Diversionary Option For Serious Felony Offenders, Jane Mitchell

Kentucky Law Journal

No abstract provided.

New Insights Into The Ovulatory Process In The Human Ovary, Misung Jo, Mats Brännström, James W. Akins, Thomas E. Curry

UK CARES Faculty Publications

BACKGROUND: Successful ovulation is essential for natural conception and fertility. Defects in the ovulatory process are associated with various conditions of infertility or subfertility in women. However, our understanding of the intra-ovarian biochemical mechanisms underlying this process in women has lagged compared to our understanding of animal models. This has been largely due to the limited availability of human ovarian samples that can be used to examine changes across the ovulatory period and delineate the underlying cellular/molecular mechanisms in women. Despite this challenge, steady progress has been made to improve our knowledge of the ovulatory process in women by: (i) …

Evaluating Experiential Learning In Undergraduate Agricultural Education: A Content Analysis Of Course Syllabi From A College Of Agriculture, Tyler Newberry

Theses and Dissertations--Community & Leadership Development

This study evaluated the presence and depth of experiential learning within undergraduate course syllabi from the Martin-Gatton College of Agriculture, Food, and Environment. Through a content analysis of 41 syllabi across five academic majors, the study examined how course objectives and assignments aligned with Kolb’s Experiential learning cycle (1984), Webb’s depth of knowledge (1997), and Joplin’s Mini to maxi Scale (1981). A structured coding instrument was used to quantify the frequency of learning elements across these three frameworks. The findings revealed that Abstract Conceptualization and Reflective Observation were the most common stages in both objectives and assignments, while Concrete Experience …

Clinicians Perspective On The Implementation Of Person-Centered Care In Persons With Aphasia, Carmen S. Alderman

Theses and Dissertations--Communication Sciences and Disorders

This paper examines speech-language pathologist perspective on implementing person-centered care for persons with aphasia. Person-centered care is based on the social model, which emphasizes the importance of including the client. The survey addressed clinicians’ use of person-centered care related to collaborative goal setting and treatment planning, as well as facilitators and barriers to implementing person-centered care for individuals with aphasia based on the view of practicing clinicians across a variety of settings. The results indicated most speech-language pathologists have heard of person-centered care, understand the importance of involving clients in goal setting, ask clients about their preferences when setting goals, …

Exploring Early Childhood Educators’ Perceptions Of Lockdown Drills: Preparedness, Training Needs, And The Balance With Social-Emotional Development, Juniper Alaska Brewer

Theses and Dissertations--Early Childhood, Special Education, and Counselor Education

The purpose of this study was to evaluate early childhood educators’ perceptions of lockdown drills. It assessed feelings of preparedness, perceived training needs, and balancing safety protocols with children's social-emotional development. A multi-methods online survey was distributed to 13 early childhood educators and students in the Interdisciplinary Early Childhood Education (IECE) field, collecting both quantitative and qualitative responses via Qualtrics.

Survey data showed educators strongly agreed drills prepare them for emergencies (average 4.46/5) and generally agreed drills prepare children (4.08/5), though responses were more neutral about reducing educator anxiety (3.77/5). Only one participant reported occasional training; most (7 of 13) …

Biomedical Relation Extraction In The Era Of (Large) Language Models, Yuhang Jiang

Theses and Dissertations--Computer Science

Biomedical relation extraction is a key task in information extraction supporting downstream applications such as knowledge discovery, information retrieval, and question answering. As such, improvements in relation extraction (RE) are expected to have major implications for other information needs in biomedicine. In this dis- sertation, we pursue biomedical relation extraction in several advanced settings with recent advances in large language models (LLMs) and retrieval augmented generation (RAG). First, we address an important slot filling task on social media that arose during the Covid-19 pandemic, using the idea of continuous prompts with encoder models. Next, we handle a tricky dynamic n-ary …

Efficacy Of Deferoxamine Mesylate In Serum And Serum-Free Media: Adult Ventral Root Schwann Cell Survival Following Hydrogen Peroxide-Induced Cell Death, Yee Hang Ethan Ma, Abhinay R. Putta, Cyrus H. H. Chan, Stephen R. Vidman, Paula V. Monje, Giles W. Plant

Neurosurgery Faculty Publications

Schwann cell (SC) transplantation shows promise in treating spinal cord injury as a pro-regenerative agent to allow host endogenous neurons to bridge over the lesion. However, SC transplants face significant oxidative stress facilitated by ROS in the lesion, leading to poor survival. deferoxamine mesylate (DFO) is a neuroprotective agent shown to reduce H₂O₂-induced cell death in serum-containing conditions. Here we show that DFO is not necessary to induce neuroprotection under serum-free conditions by cell survival quantification and phenotypic analysis via immunohistochemistry, Hif1α and collagen IV quantification via whole cell corrected total cell fluorescence, and cell death …

La Parole Comme Outil De Résistance Contre Les Tabous Oppressifs Des Femmes Marocaines : Une Étude Comparative De Paroles D’Honneur Et Hshouma, Roseline Adeleke

Students' Supported Scholarship

No abstract provided.

Design And Comparative Analysis Of Electric Motors With “Flux-Switching” Effect Having Reluctance Rotors And Pm Or Dc Stator Excitation, Oluwaseun A. Badewa, Ali Mohammadi, Donovin D. Lewis, Somasundaram Essakiappan, Madhav Manjrekar, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

This paper introduces innovative designs for synchronous electric motors with phase coils and permanent magnets (PM) or DC-excitation coils embedded in the stator. Alongside concentrated phase coils in dedicated slots, the spoke-type PMs offer high flux intensification, while the option for DC-excitation coils eliminates demagnetization risks. Since the rotor has no active electromagnetic components, the machine can achieve high-speed operation while enabling advanced cooling systems focused solely on the stator. A special implementation with a “wave” or “serpentine” DC-excitation winding which has the potential for reduced losses depending on the motor aspect ratio is presented. The operation, control, and polarity …

Harmonic And Non-Linear Effects On The Parameters And Performance Of A Vernier Machine With Flux Concentrating Spoke Rotor, Ali Mohammadi, Yaser Chulaee, Aaron M. Cramer, Ion G. Boldea, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

This paper presents a systematic study on the MAGNUS machine, which is an innovative dual-stator axial flux permanent magnet vernier machine (AFPMVM). The MAGNUS machine features a novel single-wound dual-stator design with minimal slots and an exceptionally high-polarity spoke-type permanent magnet rotor, which enables a very high flux concentration ratio. The operating principle of vernier machines is derived, showing the possible slot-pole combinations. Inductance components are introduced, and multiple methods are employed to determine the direct and quadrature axis inductances, revealing that despite its spoke-type rotor, due to a high harmonic content and a high differential leakage inductance, the MAGNUS …

Third Party Negotiated Hospital Pricing In Rural And Urban Hospitals Within 12 U.S. States, Lindsey R. Hammerslag, Aaron P. Smith, Jeffrey C. Talbert

Rural & Underserved Health Research Center Publications

No abstract provided.

Longitudinal Relationship Between Problematic Social Networking Use And Internalizing Problems, Lindsay Roesel

Theses and Dissertations--Family Sciences

The purpose of this study is to examine the longitudinal and bidirectional relationships between problematic social networking use (PSNU) and internalizing problems (depressive symptoms and self-esteem) in adolescents. Data were collected from N = 360 adolescents from two public high schools in Turkey, 14.58 years old in 9^th grade at Time 1 (54.3% females). Participants completed four assessments regarding problematic social media use, depression and self-esteem, over the course of two school years, once per semester in 9^th and 10^th grades. The study tested a series of hypotheses which tested the bidirectional relationships between PSNU on depressive …

Precision Public Health Alliances As A Model And Method For Community Engagement, Margaret L. Mcgladrey, Mary Elizabeth Lacy, Kristen Mcquerry, Rachel Hogg-Graham, Svetla Slavova, Emily R. Clear, Kory Heier, Caitline Phan, Megan Hall, Ryan Parker, Mindy Keeton, Jennifer Sword, Thomas Ard, Charbel Salem

Epidemiology and Environmental Health Faculty Publications

Using precision analytics approaches with population health data helps identify localized patterns of social determinants and comorbidities, supporting the design of tailored interventions. The University of Kentucky College of Public Health (UKCPH) and UK King’s Daughters (UKKD) have partnered to create a Precision Public Health Alliance (PPHA) applying precision analytics to UKKD electronic health records (EHR) as well as secondary datasets to map social, demographic, and clinical comorbidity factors onto colorectal cancer (CRC) screening data in UKKD’s rural service area (the northeastern Kentucky counties of Boyd, Carter, Greenup, and Lawrence and southeast Ohio county of Lawrence). In addition to UKKD …

Listening To Cursive: The Roles Of Regional Identity And Folk Beliefs In Perceptual Cue-Weighting, Stella Takvoryan

Theses and Dissertations--Linguistics

Listeners use knowledge acquired from experiences to weight phonetic-level cues to phonological contrasts. Phonetic knowledge is imbued with expectations about the contexts in which cues covary, speakers of a variety, and identity categories or constructs, so it can be challenging to distinguish expectations about variation from expectations about speakers. This study aims to explore the extent that expectations about iconic vowel quality variation in Southern American Englishes can drive cue-weighting. Listeners categorized stimuli with vowel qualities synthesized from an [ɛ]-[eɪ] continuum from a Southern and a non-Southern talker, with duration manipulated in tandem. Half of the participants saw a label …

The Effect Of Fructose Metabolism On Hepatic De Novo Lipogenesis And Adipose Tissue Insulin Resistance, Taghreed Ibrahim Fadhul

Theses and Dissertations--Nutritional Sciences

There is a worldwide epidemic of obesity and metabolic dysfunction. The root cause of obesity is driven by a positive energy balance, which is a consequence of increased caloric intake and decreased energy expenditure. Energy intake is the more dominant force, as increasing energy expenditure is a time-consuming and labor-intensive process. The caloric surplus can be mainly accounted for by increased consumption of high-fat and high-sugar diets. While the risks associated with fat consumption are well-documented, the impact of sugar intake is less understood.

Fructose is a simple sugar widely consumed in modern diets. Its intake has been associated with …

Spermidine And Polyamine Blockade As Key Mediators Of Intestinal Epithelial Cell Functions, Madison Lynn Flory

Theses and Dissertations--Microbiology, Immunology, and Molecular Genetics

Frequently, alterations in the composition of the gut microbiota and its metabolites are linked with intestinal diseases, including inflammatory bowel disease and colorectal cancer. However, the direct connections between alterations in the microbiome and disease states often remain elusive. Prior research has identified increases in both polyamines, which are a class of microbial metabolite, and in the abundance of bacteria that can produce polyamines, in a murine colorectal cancer model. Understanding the significance of the interaction between these microbial metabolites and intestinal epithelial cells (IECs) will provide new and expanded evidence of the importance of the gut microbiome in colorectal …

Visual Legal Rhetoric In The Age Of Generative Ai And Deepfakes: Renaissance Or Dark Ages?, Michael D. Murray

Law Faculty Scholarly Articles

The paradoxical development of visual generative AI tools, such as OpenAI’s DALL-E 3, Midjourney, and Stable Diffusion, simultaneously signals a renaissance and a potential dark age in visual rhetoric and communication. On the one hand, these tools democratize the creation of visual content, empowering attorneys and others to become artists and illustrators of their legal communications without needing to learn how to draw. These AI systems can simplify complex legal concepts, bridge language barriers, and enhance advocacy. But on the other hand, the proliferation of deepfakes presents significant challenges for visual rhetoric. Deepfakes can quickly and easily create realistic but …

Framing Large Language Models: Teaching Foundational Concepts Of Generative Ai And Information Literacy For Critical Student Engagement, Isaac Wink, Jennifer Hootman

Library Faculty and Staff Publications

This chapter situates the emerging scholarly conversation around AI literacy within the established Association for College and Research Libraries (ACRL) Framework for Information Literacy for Higher Education. We apply three frames in particular: 1) Authority Is Constructed and Contextual, 2) Information Creation as a Process, and 3) Research as Inquiry, considering competencies relevant to students encountering generative AI applications in educational contexts and addressing ways that both students and instructors may seek to engage productively with such tools.

This chapter primarily focuses on tools such as ChatGPT that are built atop large language models (LLMs)—systems trained on massive text corpora—rather …

Storage Media Prioritisation With Technical And Archival Appraisal, Andrew Mcdonnell, Megan M. Mummey

Library Faculty and Staff Publications

This spreadsheet tool is designed to provide archives and libraries a means of prioritizing the order in which data is migrated from physical media carriers and other sources in their collection in need of preservation. It is an adaptation of work from the Archives and Special Collections at the University of Glasgow, who developed a methodology for prioritizing archival processing of digital collections stored in physical storage media.

The original tool (downloadable here: https://researchdata.gla.ac.uk/1634/ ) permits users to calculate a priority score by weighting the following factors: the DPC BitList Endangered Digital Media score, the age of the media, its …

Quieting The Cacophony: How To Reduce Greenwashing And Environmental Damage From The Food Industry, Alex Woodward

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

In this article, Alex G. Woodward addresses the problem of greenwashing and environmental harm caused by industrial agriculture and misleading food labeling. Woodward explains that while agriculture is essential to feeding a growing global population, its large-scale, profit-driven practices cause significant environmental damage. Woodward argues for a strategy that compels transparency through warning labels and expanding credible certification frameworks. Woodward proposes that the USDA implement a tiered labeling system under its Organic Program and require factual, noncontroversial disclosures about environmental harms on food packaging, with the goal of informing consumers, restoring trust, and incentivizing environmentally responsible agricultural practices.

This Land Is Our Land: Addressing Foreign Ownership Of Agricultural Land In The United States, Will Miller

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

In this note, Will Miller examines the rapid increase in foreign ownership of U.S. agricultural land and the national security and food security risks it creates. He explains that the Agricultural Foreign Investment Disclosure Act (AFIDA) only requires reporting of foreign ownership but imposes no restrictions, leaving regulation largely to a patchwork of state laws. Miller argues for federal oversight, contending that matters of foreign affairs and national security are best handled at the national level. He proposes a comprehensive federal regulatory scheme which would set nationwide minimum standards such as prohibiting ownership by foreign adversaries and capping total foreign-held …

The Working Student Problem: Why Unpaid Interns In The Kentucky Equine Industry Need Greater Statutory Protection, Sarah Turri

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

In this note, Sarah Turri explores how working students in Kentucky’s equine industry are treated like unpaid interns, working long hours for little or no pay. She explains that this leaves them without minimum wage or civil rights protections and worsens inequality in the equestrian sport. Turri argues for extending statutory protections to working students and proposes that Kentucky follow other states in safeguarding these vulnerable workers.

The Impact Of Veterinary Stewardship Program Implementations On Backyard Chicken Farming In Rural Georgia, Lauren Ashley Herrin

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

In this article, Lauren Ashley Herrin examines the impacts of new FDA veterinary stewardship regulations on backyard chicken farming in rural Georgia. She explains that requiring prescriptions for previously over-the-counter antibiotics disproportionately harms low-income and rural communities by limiting access to affordable veterinary care, worsening risks of disease spread and public health threats. Herrin argues for policy approaches that balance antimicrobial resistance concerns with the realities of rural accessibility. She proposes solutions such as telehealth veterinary consultations, scholarship programs to encourage rural veterinary practice, and expanding prescriptive authority for veterinary technicians to ensure sustainable animal health care in underserved areas

When Breathing Becomes Wheezing: Why Kentucky Should Require Schools To Conduct Radon Testing, Abbey Norvell

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

In this note, Abbey Norvell analyzes the dangers of radon exposure in Kentucky schools, highlighting the state’s nation-leading lung cancer rates and the heightened risks for children. She explains that Kentucky has no law requiring schools to test for or mitigate radon levels, leaving students vulnerable. Norvell argues for mandatory radon testing and remediation in Kentucky schools. She proposes legislation modeled on other states requiring testing in both public and private schools within five years, and remediation within two years if levels exceed EPA standards.

Pesticide Lies! A Call For Epa Action As Recent Judicial Decisions Reveal The Harmful Truth Resulting From Exposure, Stevi Whitman

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

In this note, Stevi Whitman analyzes the dangers of weak pesticide regulation in the United States. Whitman explains that the EPA’s reliance on the vague “unreasonable adverse effects” standard under the Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) allows harmful products like Roundup to remain on the market despite strong evidence of health risks. Whitman argues for strengthening U.S. pesticide laws to match the European Union’s stricter safety threshold. She proposes amending FIFRA to require proactive bans on hazardous pesticides and greater accountability from manufacturers to better protect public health and the environment.

Analysing The Us—China “Ai Cold War” Narrative, Yujia He, Richard Heeks

Patterson School of Diplomacy and International Commerce Faculty Publications

Discussions about artificial intelligence (AI) are gaining prominence in the recent revival of “cold war” narratives comparing US-China relations today to the historical rivalry between the US and the Soviet Union. Drawing on a review of existing academic and policy literature engaging with the “AI cold war” narrative, this paper examines how the narrative is justified, and numerous ways that it can be challenged. It finds that the framing is largely driven by the securitisation of AI, as state actors and policy pundits view AI innovations’ dual-use capabilities as key to national security and ideological competition. However, critics posit that …

The New American Farmer – Extension Engagement With Urban Agriculture And Food Systems, Margaux S.C. Robinson, Kc V. Cunningham, Jeffery A. Young, Nicole D. Breazeale, Kenneth Jones, Julie Zimmerman

Sociology Faculty Publications

Extension's evolving role in urban food production will require intensive reflexivity and ongoing collaboration. Extension educators around the country have already made progress in engaging with both the social and horticultural sides of urban agriculture. Designed appropriately, urban food systems hold the potential for healthy food access, community and environmental resilience, and economic prosperity (Rangarajan & Riordan 2019). Moving forward, we offer recommendations for Extension staff to apply within their institutions and beyond. Specifically, we urge Extension to prioritize the following: 1) mediate the rural/urban dichotomy, 2) tackle structural and institutional power dynamics, and 3) intensify strategies for community resilience.

Community-Engaged Approach To Improve Food Access And Consumption Of Fruits And Vegetables In A Rural Appalachian Community, Courtney Luecking, Makenzie Barr, Dawn Brewer, Kathryn M. Cardarelli

Dietetics and Human Nutrition Faculty Publications

Background/Objectives: Community–academic partnerships offer unique opportunities to leverage expertise and resources to modify structural factors that address community priorities. However, few in-depth examples of successful partnerships in rural communities to improve food security are available. This manuscript describes the process of building a partnership to reduce food insecurity in a rural Kentucky community.

Methods: The research team worked with Cooperative Extension to form a community advisory board (CAB) representative of diverse sectors of the community who had interest in food security, agriculture, and/or nutrition. The CAB convened regularly, in-person or virtually, to review community assessment information and identify, select, and …

Axial Flux Permanent Magnet Generators For Direct-Drive Wind Turbines - Review And Optimal Design Studies, Matin Vatani, Ali Mohammadi, Donovin D. Lewis, John F. Eastham, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

This paper presents a comprehensive analysis of the feasibility, cost, and electromagnetic performance of five distinct types of axial flux permanent magnet (AFPM) generators designed for direct-drive wind turbines. The generator configurations investigated include a single-sided AFPM generator with a surface-mounted PM rotor (AFPMG), a double-sided AFPM generator featuring PMs on the stator and a reluctance rotor (AFPMG-RR), a coreless stator AFPM generator with surface PMs (CAFPMG-SPM), and a coreless stator AFPM generator with a Halbach PM array rotor (CAFPMG-Hal). Each generator’s operating principles and configurations are thoroughly explained and compared. Large-scale multi-objective design optimizations were conducted on each type, …