Digital Commons Network^™

Meeting The Social Media Needs Of Lexington’S Lgbtq Community, Erin Weber

LIS Student Conference

Erin will present a strategic plan for the social media needs of the Pride Community Services Organization (PCSO) and Pride Library in Lexington. She will include discussion on whether social media is a means to a larger goal or a means unto itself for PCSO.

More Than Just Students: Personal Information Needs Of Esl Community College Students, Jennifer Jeffers

LIS Student Conference

Over a million international students attended U.S. colleges and universities in the 2015-­ 2016 academic year, international student enrollment has risen steadily over the past decade, and the majority of these students come from countries where English is not the primary language (Institute for International Education, 2016). Furthermore, the Pew Research Center reports that “future immigrants and their children will account for 88% of the U.S. population increase between 2016 and 2065” (as cited in Ramakrishnan, Barker, Vervoordt, & Zhang, 2017, p. 1).

Existing studies of international and English as a second language (ESL) students have focused mainly on their …

Multi-Mw Solar Pv Pumping System With Capacity Modulation And Battery Voltage Support, Oluwaseun M. Akeyo, Vandana Rallabandi, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

Solar photovoltaic (PV) renewable energy systems are undergoing major technological developments and large-scale field deployment and electric grid integration. This paper proposes a method of expanding the capacity of an existing irrigation farm with additional pumps powered by solar PV. The system includes PV arrays and battery energy storage connected to a common dc bus, which energizes an array of variable speed inverter driven pumps. Capacity modulation is achieved by energizing an optimal number of pumps required in order to meet a particular load demand with minimum supply energy. A grid connection to the dc bus of the power electronic …

Forage News [2017-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Don't Let Potash Limit Your Forages
• Cyanide Poisoning in Ruminants
• Web Survey
• Summary of 2017 KY Grazing Conference
• Fall Pasture Evaluation
• Is My Alfalfa Safe to Graze?

Community Leadership Institute Of Kentucky, Beth Bowling, Frances J. Feltner, Nancy E. Schoenberg, Ernie Scott, Gia Mudd-Martin

Center of Excellence in Rural Health Presentations

The Community Leadership Institute of Kentucky(CLIK) aims to improve community research capacity to address health disparities in communities, particularly Appalachia.

Established in 2014, through a partnership of the UK Center of Excellence in Rural Health, the UK Center for Clinical and Translational Science Community Engagement Program, and the Kentucky Office of Rural Health, the intensive four-week training provides:

• Training in research and leadership
• Funding for community research projects
• Technical support for up to one year as participants implement community research projects

Up to 12 slots are available annually, with priority given to leaders from Appalachian Kentucky and to projects related …

Language Changes, But Should Legal Writing Change With It?, Diane B. Kraft

Law Faculty Popular Media

No abstract provided.

The Effect Of Co2, Intracellular Ph And Extracellular Ph On Mechanosensory Proprioceptor Responses In Crayfish And Crab, Viresh Dayaram, Cole A. Malloy, Sarah Martha, Brenda Alvarez, Ikenna Chukwudolue, Nadera Dabbain, Dlovan D.Mahmood, Slavina Goleva, Tori Hickey, Angel Ho, Molly King, Paige Kington, Matthew Mattingly, Samuel Potter, Landon Simpson, Amanda Spence, Henry Uradu, Jacob Van Doorn, Dlovan Faiq, Robin L. Cooper

Biology Faculty Publications

Proprioceptive neurons monitor the movements of limbs and joints to transduce the movements into electrical signals. These neurons function similarly in species from arthropods to humans. These neurons can be compromised in disease states and in adverse environmental conditions such as with changes in external and internal pH. We used two model preparations (the crayfish muscle receptor organ and a chordotonal organ in the limb of a crab) to characterize the responses of these proprioceptors to external and internal pH changes as well as raised CO₂. The results demonstrate the proprioceptive organs are not highly sensitive to changes …

Development Of Halofluorochromic Polymer Nanoassemblies For The Potential Detection Of Liver Metastatic Colorectal Cancer Tumors Using Experimental And Computational Approaches, Derek Alexander Reichel, Louis T. Curtis, Elizabeth Ehlman, B. Mark Evers, Piotr G. Rychahou, Hermann B. Frieboes, Younsoo Bae

Pharmaceutical Sciences Faculty Publications

Purpose—To develop polymer nanoassemblies (PNAs) modified with halofluorochromic dyes to allow for the detection of liver metastatic colorectal cancer (CRC) to improve therapeutic outcomes.

Methods—We combine experimental and computational approaches to evaluate macroscopic and microscopic PNA distributions in patient-derived xenograft primary and orthotropic liver metastatic CRC tumors. Halofluorochromic and non-halofluorochromic PNAs (hfPNAs and n-hfPNAs) were prepared from poly(ethylene glycol), fluorescent dyes (Nile blue, Alexa546, and IR820), and hydrophobic groups (palmitate), all of which were covalently tethered to a cationic polymer scaffold [poly(ethylene imine) or poly(lysine)] forming particles with an average diameter < 30 nm.

Results—Dye-conjugated PNAs showed no aggregation under …

Challenges Towards Revitalizing Hemp: A Multifaceted Crop, Craig M. Schluttenhofer, Ling Yuan

Plant and Soil Sciences Faculty Publications

Hemp has been an important crop throughout human history for food, fiber, and medicine. Despite significant progress made by the international research community, the basic biology of hemp plants remains insufficiently understood. Clear objectives are needed to guide future research. As a semi-domesticated plant, hemp has many desirable traits that require improvement, including eliminating seed shattering, enhancing the quantity and quality of stem fiber, and increasing the accumulation of phytocannabinoids. Methods to manipulate the sex of hemp plants will also be important for optimizing yields of seed, fiber, and cannabinoids. Currently, research into trait improvement is hindered by the lack …

Linking Tuberous Sclerosis Complex, Excessive Mtor Signaling, And Age-Related Neurodegeneration: A New Association Between Tsc1 Mutation And Frontotemporal Dementia, Nicholas T. Olney, Carolina Alquezar, Eliana Marisa Ramos, Alissa L. Nana, Jamie C. Fong, Anna M. Karydas, Joanne B Taylor, Melanie L. Stephens, Andrea R. Argouarch, Victoria A. Van Berlo, Deepika R. Dokuru, Elliott H. Sherr, Gregory A. Jicha, William P. Dillon, Rahul S. Desikan, Mary De May, William W. Seeley, Giovanni Coppola, Bruce L. Miller, Aimee W. Kao

Neurology Faculty Publications

No abstract provided.

Mtor Kinase Inhibition Effectively Decreases Progression Of A Subset Of Neuroendocrine Tumors That Progress On Rapalog Therapy And Delays Cardiac Impairment, Melissa A. Orr-Asman, Zhengtao Chu, Min Jiang, Mariah Worley, Kathleen Lesance, Sheryl E. Koch, Vinicius S. Carreira, Hanan M. Dahche, David R. Plas, Kakajan Komurov, Xiaoyang Qi, Carol A. Mercer, Lowell B. Anthony, Jack Rubinstein, Hala E. Thomas

Internal Medicine Faculty Publications

Inhibition of mTOR signaling using the rapalog everolimus is an FDA-approved targeted therapy for patients with lung and gastroenteropancreatic neuroendocrine tumors (NET). However, patients eventually progress on treatment, highlighting the need for additional therapies. We focused on pancreatic NETs (pNET) and reasoned that treatment of these tumors upon progression on rapalog therapy, with an mTOR kinase inhibitor (mTORKi), such as CC-223, could overcome a number of resistance mechanisms in tumors and delay cardiac carcinoid disease. We performed preclinical studies using human pNET cells in vitro and injected them subcutaneously or orthotopically to determine tumor progression and cardiac function in mice …

Improving The Baculovirus Expression Vector System With Vankyrin-Enhanced Technology, Kendra H. Steele, Barbara J. Stone, Kathleen M. Franklin, Angelika Fath-Goodin, Xiufeng Zhang, Haobo Jiang, Bruce A. Webb, Christoph Geisler

Entomology Faculty Publications

The baculovirus expression vector system (BEVS) is a widely used platform for the production of recombinant eukaryotic proteins. However, the BEVS has limitations in comparison to other higher eukaryotic expression systems. First, the insect cell lines used in the BEVS cannot produce glycoproteins with complex‐type N‐glycosylation patterns. Second, protein production is limited as cells die and lyse in response to baculovirus infection. To delay cell death and lysis, we transformed several insect cell lines with an expression plasmid harboring a vankyrin gene (P‐vank‐1), which encodes an anti‐apoptotic protein. Specifically, we transformed Sf9 cells, Trichoplusia ni High Five …

"When Words Become Unclear": Unmasking Ict Through Visual Methodologies In Participatory Ict4d, Caitlin M. Bentley, David Nemer, Sara Vannini

Information Science Faculty Publications

Across the globe, our work and social lives are increasingly integrated with Information and Communication Technologies (ICT), yet massive disparities in the values, uses and benefits of ICT exist. New methods are needed to shed light on unique and integrative concepts of ICT across cultures. This paper explores the use of visual methods to facilitate critical engagement with ICT—defined as situational awareness, reflexive ICT practice and power and control over ICT. This definition of critical ICT engagement is informed by a cultural identity lens, and intends to improve participatory methods in ICT for Development (ICT4D) and community technology design and …

Co-Opting Atp-Generating Glycolytic Enzyme Pgk1 Phosphoglycerate Kinase Facilitates The Assembly Of Viral Replicase Complexes, K. Reddisiva Prasanth, Chingkai Chuang, Peter D. Nagy

Plant Pathology Faculty Publications

The intricate interactions between viruses and hosts include exploitation of host cells for viral replication by using many cellular resources, metabolites and energy. Tomato bushy stunt virus (TBSV), similar to other (+)RNA viruses, induces major changes in infected cells that lead to the formation of large replication compartments consisting of aggregated peroxisomal and ER membranes. Yet, it is not known how TBSV obtains the energy to fuel these energy-consuming processes. In the current work, the authors discovered that TBSV co-opts the glycolytic ATP-generating Pgk1 phosphoglycerate kinase to facilitate the assembly of new viral replicase complexes. The recruitment of Pgk1 into …

The Transformation Of Science With Hpc, Big Data, And Ai, Jeffrey Kirk

Commonwealth Computational Summit

High performance computing has matured into an indispensable tool for not only academic research and government labs and agencies, but also for many industry sectors: energy, manufacturing, healthcare, financial services, even digital content creation. More recently, the advent of Big Data has enabled the use of HPC techniques for large scale data analysis, expanding the scope of HPC and the reach of it into more research and enterprise use cases. Since 2012, a new regime of data-driven analytics, deep learning, has erupted in popularity, fueled by both the massive performance increases in HPC technologies and in the explosive rate of …

Tell Me Why? Tell Me More! Explaining Predictions, Iterated Learning Bias, And Counter-Polarization In Big Data Discovery Models, Olfa Nasraoui

Commonwealth Computational Summit

Outline:

What can go Wrong in Machine Learning?

• Unfair Machine Learning
• Iterated Bias & Polarization
• Black Box models

Tell me more: Counter-Polarization

Tell me why: Explanation Generation

Cloud‐Based Text Analytics Harvesting, Cleaning And Analyzing Corporate Earnings Conference Calls, Michael Chuancai Zhang, Vikram Gazula, Dan Stone, Hong Xie

Commonwealth Computational Summit

No abstract provided.

Evolution Of Bias In Human And Machine Learning Algorithm Interaction, Wenlong Sun, Olfa Nasraoui, Patrick Shafto

Commonwealth Computational Summit

Human algorithm interaction:

• people are now affected by the output of all types of machine learning algorithms.
• social media, blogs, social networks, and other services and applications.

Motivation:

• ML algorithm relied on reliable labels from experts to build prediction.
• However, ML algorithm started to receive data from the more general population.
• The interaction leads to biased result which is caused by ingesting unchecked information from general population, such as biased samples and biased labels.

Nanoparticle Delivery Of Mir-34a Eradicates Long-Term-Cultured Breast Cancer Stem Cells Via Targeting C22orf28 Directly, Xiaoti Lin, Weiyu Chen, Fengqin Wei, Binhua P. Zhou, Mien-Chie Hung, Xiaoming Xie

Molecular and Cellular Biochemistry Faculty Publications

Rationale: Cancer stem cells (CSCs) have been implicated as the seeds of therapeutic resistance and metastasis, due to their unique abilities of self-renew, wide differentiation potentials and resistance to most conventional therapies. It is a proactive strategy for cancer therapy to eradicate CSCs. Methods: Tumor tissue-derived breast CSCs (BCSC), including XM322 and XM607, were isolated by fluorescence-activated cell sorting (FACS); while cell line-derived BCSC, including MDA-MB-231.SC and MCF-7.SC, were purified by magnetic-activated cell sorting (MACS). Analyses of microRNA and mRNA expression array profiles were performed in multiple breast cell lines. The mentioned nanoparticles were constructed following the standard molecular cloning …

Glycolytic Reprogramming Through Pck2 Regulates Tumor Initiation Of Prostate Cancer Cells, Jiangsha Zhao, Jieran Li, Teresa W.M. Fan, Steven X. Hou

Toxicology and Cancer Biology Faculty Publications

Tumor-initiating cells (TICs) play important roles in tumor progression and metastasis. Identifying the factors regulating TICs may open new avenues in cancer therapy. Here, we show that TIC-enriched prostate cancer cell clones use more glucose and secrete more lactate than TIC-low clones. We determined that elevated levels of phosphoenolpyruvate carboxykinase isoform 2 (PCK2) are critical for the metabolic switch and the maintenance of TICs in prostate cancer. Information from prostate cancer patient databases revealed that higher PCK2 levels correlated with more aggressive tumors and lower survival rates. PCK2 knockdown resulted in low TIC numbers, increased cytosolic acetyl-CoA and cellular protein …

No Difference In Myosin Kinetics And Spatial Distribution Of The Lever Arm In The Left And Right Ventricles Of Human Hearts, Divya Duggal, S. Requena, Janhavi Nagwekar, Sangram Raut, Ryan Rich, Hriday Das, Vipul Patel, Ignacy Gryczynski, Rafal Fudala, Zygmunt Gryczynski, Cheavar Blair, Kenneth S. Campbell, Julian Borejdo

Physiology Faculty Publications

The systemic circulation offers larger resistance to the blood flow than the pulmonary system. Consequently, the left ventricle (LV) must pump blood with more force than the right ventricle (RV). The question arises whether the stronger pumping action of the LV is due to a more efficient action of left ventricular myosin, or whether it is due to the morphological differences between ventricles. Such a question cannot be answered by studying the entire ventricles or myocytes because any observed differences would be wiped out by averaging the information obtained from trillions of myosin molecules present in a ventricle or myocyte. …

Image-Based Analysis To Dissect Vertical Distribution And Horizontal Asymmetry Of Conspecific Root System Interactions In Response To Planting Densities, Nutrients And Root Exudates In Arabidopsis Thaliana, Jane Geisler-Lee, Xian Liu, Wei Rang, Jayanthan Raveendiran, Marisa Blake Szubryt, David John Gibson, Matt Geisler, Qiang Cheng

Institute for Biomedical Informatics Faculty Publications

Intraspecific competition is an important plant interaction that has been studied extensively aboveground, but less so belowground, due to the difficulties in accessing the root system experimentally. Recent in vivo and in situ automatic imaging advances help understand root system architecture. In this study, a portable imaging platform and a scalable transplant technique were applied to test intraspecific competition in Arabidopsis thaliana. A single green fluorescent protein labeled plant was placed in the center of a grid of different planting densities of neighboring unlabeled plants or empty spaces, into which different treatments were made to the media. The root …

Silver Engineered Nanomaterials And Ions Elicit Species-Specific O2 Consumption Responses In Plant Growth Promoting Rhizobacteria, Ricky W. Lewis, Jason M. Unrine, Paul M. Bertsch, David H. Mcnear Jr.

Plant and Soil Sciences Faculty Publications

Metal containing engineered nanomaterials (ENMs) are now commonly used in various industrial and commercial applications. Many of these materials can be transformed during waste water treatment and ultimately enter terrestrial ecosystems via agriculturally applied biosolids. It is unclear how agriculturally important soil microbes will be affected by exposure to environmentally relevant, sublethal concentrations of ENMs and their transformation products (i.e., ions, aggregates, etc.). A method was developed, which puts O₂ consumption responses in terms of viability, and tested by examining the toxic effects of Ag⁺, Zn²⁺, and Ni²⁺ ions on the plant growth promoting …

Magnus--An Ultra-High Specific Torque Pm Axial Flux Type Motor With Flux Focusing And Modulation, Vandana Rallabandi, Narges Taran, Dan M. Ionel, Ion G. Boldea

Power and Energy Institute of Kentucky Faculty Publications

Axial flux permanent magnet (AFPM) synchronous motors are attractive for low speed direct drive systems because of their high specific torque. The present paper proposes a novel axial flux machine topology, called MAGNUS, obtained by combining a yokeless central stator type NS arrangement with two external high polarity spoke-rotors, resulting in very high flux concentration. The stator, which includes main and auxiliary teeth for air-gap profiling has a small number of concentrated coils. Two stator configurations are discussed, the first, a yokeless construction with coils wound around the teeth, and the second with coils placed around the stator core in …

1H, 15N, And 13C Chemical Shift Assignments Of The Regulatory Domain Of Human Calcineurin, Dinesh K. Yadav, Sri Ramya Tata, John Hunt, Erik C. Cook, Trevor P. Creamer, Nicholas C. Fitzkee

Center for Structural Biology Faculty Publications

Calcineurin (CaN) plays an important role in T-cell activation, cardiac system development and nervous system function. Previous studies have demonstrated that the regulatory domain (RD) of CaN binds calmodulin (CaM) towards the N-terminal end. Calcium-loaded CaM activates the serine/threonine phosphatase activity of CaN by binding to the RD, although the mechanistic details of this interaction remain unclear. It is thought that CaM binding at the RD displaces the auto-inhibitory domain (AID) from the active site of CaN, activating phosphatase activity. In the absence of calcium-loaded CaM, the RD is disordered, and binding of CaM induces folding in the RD. In …

Inductance Testing According To The New Ieee Std 1812-Application And Possible Extensions For Ipm Machines, Vandana Rallabandi, Narges Taran, Dan M. Ionel, Ping Zhou

Power and Energy Institute of Kentucky Faculty Publications

Equivalent circuit parameters are very important for permanent magnet synchronous machines since they serve as the basis for performance estimation and implementation of power electronic drives controls. Specified in the newly approved IEEE Std 1812, a short-circuit test can be employed, in combination with an open-circuit measurement, in order to determine the back emf and the synchronous inductance. In this paper, it is shown that for interior permanent magnet (IPM) machines this approach can be used only to determine the d-axis inductance and additional and separate measurements are required for the q-axis inductance. In this respect, one method widely used …

An Empirical Study Of University Patent Activity, Brian L. Frye, Christopher J. Ryan

Law Faculty Scholarly Articles

Since 1980, a series of legislative acts and judicial decisions have affected the ownership, scope, and duration of patents. These changes have coincided with historic increases in patent activity among academic institutions.

This article presents an empirical study of how changes to patent policy precipitated responses by academic institutions, using spline regression functions to model their patent activity. We find that academic institutions typically reduced patent activity immediately before changes to the patent system, and increased patent activity immediately afterward. This is especially true among research universities. In other words, academic institutions responded to patent incentives in a strategic manner, …

Secondhand Smoke Exposure And Preclinical Markers Of Cardiovascular Risk In Toddlers, Judith A Groner, Hong Huang, Mandar S. Joshi, Nicholas Eastman, Lisa Nicholson, John Anthony Bauer

Pediatrics Faculty Publications

Objective: Links between secondhand smoke (SHS) exposure and cardiovascular disease in adults are well established but seldom reported during childhood. Although rates of smoking have decreased, young children from low-income backgrounds remain likely to be exposed to SHS. The purpose of this study was to investigate relationships between SHS exposure in young children and several preclinical markers of cardiovascular risk that have been established as relevant to adult populations.

Methods: 139 children, 2–5 years of age, were enrolled in a cross-sectional study. SHS exposure was objectively determined by hair nicotine level; a comprehensive panel of clinical markers (AM blood pressure, …

Single-Base Resolution Mapping Of 5-Hydroxymethylcytosine Modifications In Hippocampus Of Alzheimer's Disease Subjects, Elizabeth M. Ellison, Melissa A. Bradley-Whitman, Mark A. Lovell

Chemistry Faculty Publications

Epigenetic modifications to cytosine have been shown to regulate transcription in cancer, embryonic development, and recently neurodegeneration. While cytosine methylation studies are now common in neurodegenerative research, hydroxymethylation studies are rare, particularly genome-wide mapping studies. As an initial study to analyze 5-hydroxymethylcytosine (5-hmC) in the Alzheimer’s disease (AD) genome, reduced representation hydroxymethylation profiling (RRHP) was used to analyze more than 2 million sites of possible modification in hippocampal DNA of sporadic AD and normal control subjects. Genes with differentially hydroxymethylated regions were filtered based on previously published microarray data for altered gene expression in hippocampal DNA of AD subjects. Our …

Creating Space At The Table: Intellectual Freedom Can Bolster Diverse Voices, Shannon M. Oltmann

Information Science Faculty Publications

Many of the most challenged books, year after year, feature voices from diverse communities (including those of women, racial or ethnic minorities, and LGBT people). Intellectual freedom protects these voices and protects our right to hear these voices. This essay discusses the definition of intellectual freedom, why diverse perspectives are important, and how intellectual freedom can bolster diverse voices. In turn, this can improve our relationships with one another, increasing empathy and respect, which can be reflected in numerous ways and is particularly important in a divisive political climate.