Digital Commons Network^™

Stable Isotope-Resolved Metabolomics Shows Metabolic Resistance To Anti-Cancer Selenite In 3d Spheroids Versus 2d Cell Cultures, Teresa W. -M. Fan, Salim S. El-Amouri, Jessica K. A. Macedo, Qing Jun Wang, Huan Song, Teresa Cassel, Andrew N. Lane

Center for Environmental and Systems Biochemistry Faculty Publications

Conventional two-dimensional (2D) cell cultures are grown on rigid plastic substrates with unrealistic concentration gradients of O₂, nutrients, and treatment agents. More importantly, 2D cultures lack cell–cell and cell–extracellular matrix (ECM) interactions, which are critical for regulating cell behavior and functions. There are several three-dimensional (3D) cell culture systems such as Matrigel, hydrogels, micropatterned plates, and hanging drop that overcome these drawbacks but they suffer from technical challenges including long spheroid formation times, difficult handling for high throughput assays, and/or matrix contamination for metabolic studies. Magnetic 3D bioprinting (M3DB) can circumvent these issues by utilizing nanoparticles that enable …

Ca2+, Astrocyte Activation And Calcineurin/Nfat Signaling In Age-Related Neurodegenerative Diseases, Pradoldej Sompol, Christopher M. Norris

Sanders-Brown Center on Aging Faculty Publications

Mounting evidence supports a fundamental role for Ca²⁺ dysregulation in astrocyte activation. Though the activated astrocyte phenotype is complex, cell-type targeting approaches have revealed a number of detrimental roles of activated astrocytes involving neuroinflammation, release of synaptotoxic factors and loss of glutamate regulation. Work from our lab and others has suggested that the Ca²⁺/calmodulin dependent protein phosphatase, calcineurin (CN), provides a critical link between Ca²⁺ dysregulation and the activated astrocyte phenotype. A proteolyzed, hyperactivated form of CN appears at high levels in activated astrocytes in both human tissue and rodent tissue around regions of amyloid and …

High-Density Lipoprotein Inhibits Serum Amyloid A-Mediated Reactive Oxygen Species Generation And Nlrp3 Inflammasome Activation, Preetha Shridas, Maria C. De Beer, Nancy R. Webb

Internal Medicine Faculty Publications

Serum amyloid A (SAA) is a high-density apolipoprotein whose plasma levels can increase more than 1000-fold during a severe acute-phase inflammatory response and are more modestly elevated in chronic inflammation. SAA is thought to play important roles in innate immunity, but its biological activities have not been completely delineated. We previously reported that SAA deficiency protects mice from developing abdominal aortic aneurysms (AAAs) induced by chronic angiotensin II (AngII) infusion. Here, we report that SAA is required for AngII-induced increases in interleukin-1β (IL-1β), a potent proinflammatory cytokine that is tightly controlled by the Nod-like receptor protein 3 (NLRP3) inflammasome and …

Role Of Mcp-1 And Ccr2 In Ethanol-Induced Neuroinflammation And Neurodegeneration In The Developing Brain, Kai Zhang, Haiping Wang, Mei Xu, Jacqueline A. Frank, Jia Luo

Pharmacology and Nutritional Sciences Faculty Publications

Background: Neuroinflammation and microglial activation have been implicated in both alcohol use disorders (AUD) and fetal alcohol spectrum disorders (FASD). Chemokine monocyte chemoattractant protein 1 (MCP-1) and its receptor C-C chemokine receptor type 2 (CCR2) are critical mediators of neuroinflammation and microglial activation. FASD is the leading cause of mental retardation, and one of the most devastating outcomes of FASD is the loss of neurons in the central nervous system (CNS). The underlying molecular mechanisms, however, remain unclear. We hypothesize that MCP-1/CCR2 signaling mediates ethanol-induced neuroinflammation and microglial activation, which exacerbates neurodegeneration in the developing brain.

Methods: C57BL/6 mice and …

A Reckless Verisimilitude: The Archive In James Ellroy’S Fiction, Bradley J. Wiles

disClosure: A Journal of Social Theory

The archive as both plot element and narrative presentation factors significantly into the work of James Ellroy’s novels in the L.A. Quartet and USA Underworld Trilogy series. This article examines the important role of the archive as a source of information and evidence that Ellroy’s characters utilize in their attempts at either maintaining or attacking the status quo. Through these novels, Ellroy conveys the potential power archives wield over the trajectory of history and our understanding of it by demonstrating how the historical record is often shaped in favor of the powerful. Yet even if the archive is a manifestation …

Togetherness With The Past: Literary Pedagogy And The Digital Archive, Madeline B. Gangnes

disClosure: A Journal of Social Theory

Archival materials are invaluable to an understanding of the historical, cultural, and material contexts in which literary texts were published. Materiality, paratextual elements, and other key characteristics of literature cannot be discerned from recent editions. Yet original and rare versions of literary texts are difficult or impossible for most scholars, let alone their students, to access. Digital facsimiles provide opportunities to examine archival texts over the Internet, alleviating logistical and financial barriers. In Dust: The Archive and Cultural History (2001), Carolyn Steedman writes: “The Archive is a place in which people can be alone with the past” (81); archives are …

Volume 27: Archives, Sophonie Bazile, Christine L. Woodward, Zachary Griffith

disClosure: A Journal of Social Theory

The 2017-2018 Editorial Collective is pleased to present the 27th volume of disClosure: A Journal of Social Theory.

Over the past year, we have compiled an exciting collection of interviews, scholarly articles, poetry, and fiction that explore the volume’s central theme: “Archives.” Archives are dynamic constellations of absence and presence, ghosts and ghouls, dust and the digital. As such, discussions of archives stretch into multiple schools of thought and practice, raising questions about power, knowledge, memory, community, and social justice. The works collected here, each one employing its own theoretical and methodological approach to archives, contribute to these important …

To Un-Become: Between Historic Reminder And Hallucination, Geographical Document And Childhood Memory, Collective Tragedy And Personal Healing, Saša Rajšić

disClosure: A Journal of Social Theory

To Un-Become: Between Historic Reminder and Hallucination, Geographical Document and Childhood Memory, Collective Tragedy and Personal Healing is an multimedia art project, which explores the concept of un-becoming through revisiting Operation Storm in Yugoslavia and its consequences over two decades later. My interest in the concept of un-becoming was sparked by a court case in which General Ante Gotovina, a former Croatian military officer, was found guilty of organizing and implementing a permanent and forcible removal of the Serbian people in a 1995 military action entitled Operation Storm. Not long after, Gotovina’s convictions for crimes against humanity were reversed by …

The Death Of Professor Jones: Ghosts And Memory In A Small University Archives, Erin Dix

disClosure: A Journal of Social Theory

The following is a true story of hauntings, literal and figurative, at a small liberal arts college in the Midwest. It is the tale of Haunted Lawrence: a walking tour of the Lawrence University campus featuring historical stories of the ghostly and unexplained, designed and led by staff in the University Archives for the past ten years. Perennially popular with the campus community, the tour has grown to plague the university archivist. This essay is an attempt to exorcise her personal Haunted Lawrence demons.

A Stress Management App Intervention For Cancer Survivors: Design, Development, And Usability Testing, Elin Børøsund, Jelena Mirkovic, Matthew M. Clark, Shawna L. Ehlers, Michael A. Andrykowski, Anne Bergland, Marianne Westeng, Lise Solberg Nes

Behavioral Science Faculty Publications

Background: Distress is prevalent in cancer survivors. Stress management interventions can reduce distress and improve quality of life for cancer patients, but many people with cancer are unfortunately not offered or able to attend such in-person stress management interventions.

Objective: The objective of this study was to develop an evidence-based stress management intervention for patients living with cancer that can be delivered electronically with wide reach and dissemination. This paper describes the design and development process of a technology-based stress management intervention for cancer survivors, including the exploration phase, intervention content development, iterative software development (including design, development, and formative …

Seeking Glimpses: Reflections On Doing Archival Work, Alex Hanson, Stephanie Jones, Thomas Passwater, Noah Wilson

disClosure: A Journal of Social Theory

This article explores the role of archival research in understanding and generating social histories from the perspectives of four different doctoral students as they reflect on their archival research experiences. We argue that archival research is complex, subjective, contextual, and at times, incomplete. Our various perspectives address ideas of privilege, representation, what it means to remember (or forget), how archives are constituted and reconstituted, and where we can make meaning in archival spaces. This article demonstrates that although archival research has had a presence in Composition and Rhetoric for some time, that presence is continually shifting, and even when embarking …

Traditional Knowledge And Digital Archives: An Interview With Kim Christen, Kimberly Christen, Leslie Davis, Zachary Griffith, Jacob Neely

disClosure: A Journal of Social Theory

Dr. Kim Christen is a Professor in the Department of English, the Director of the Digital Technology and Culture Program, the Director of the Center for Digital Scholarship and Curation, and the Director of Digital Initiatives for the College of Arts and Sciences at Washington State University. Christen is also the Director of the Plateau Peoples’ Web Portal, a collaboratively curated site of Plateau cultural materials; Mukurtu CMS, a content management system and community digital archive platform built around the particular needs of indigenous peoples globally; and co-Director with Jane Anderson of Local Contexts, an educational website for innovative traditional …

People Of The Stacks: ‘The Archivist’ Character In Fiction, Sharon Wolff

disClosure: A Journal of Social Theory

Archives and archival professionals suffer from what may be termed as an “image problem” due to their general lack of exposure to the public. With their efforts being tucked away in various repositories, their fictional representatives become an important way to give people an idea of what they do. With the help of an article by Arlene Schmuland, two works of fiction, People of the Book by Geraldine Brooks from 2008 and The Archivist by Martha Cooley from 1998, are used to compare fictional archivists and the ways their differences may indicate a change in how their real-life counterparts are …

Power Electronics Powertrain Architectures For Hybrid And Solar Electric Airplanes With Distributed Propulsion, Damien Lawhorn, Vandana Rallabandi, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

Distributed propulsion in aircraft has been shown to increase reliability and benefit aerodynamic performance. This paper discusses power electronic architectures and proposes control schemes suitable for distributed propulsion in hybrid and electric airplanes. Hybrid electric airplanes include permanent magnet synchronous generators driven by jet engines. The output of the generators is connected to the propulsion motors through back to back voltage source converters. Batteries, connected to the DC bus through buck-boost converters, are used to provide additional power to the propulsion motors during take off and climb. In the case of electric airplanes, the jet engine-permanent magnet generator system is …

Self-Resistance During Muraymycin Biosynthesis: A Complementary Nucleotidyltransferase And Phosphotransferase With Identical Modification Sites And Distinct Temporal Order, Zheng Cui, Xia-Chang Wang, Xiaodong Liu, Anke Lemke, Stefan Koppermann, Christian Ducho, Jürgen Rohr, Jon S. Thorson, Steven G. Van Lanen

Pharmaceutical Sciences Faculty Publications

Muraymycins are antibacterial natural products from Streptomyces spp. that inhibit translocase I (MraY), which is involved in cell wall biosynthesis. Structurally, muraymycins consist of a 5′-C-glycyluridine (GlyU) appended to a 5″-amino-5″-deoxyribose (ADR), forming a disaccharide core that is found in several peptidyl nucleoside inhibitors of MraY. For muraymycins, the GlyU-ADR disaccharide is further modified with an aminopropyl-linked peptide to generate the simplest structures, annotated as the muraymycin D series. Two enzymes encoded in the muraymycin biosynthetic gene cluster, Mur29 and Mur28, were functionally assigned in vitro as a Mg·ATP-dependent nucleotidyltransferase and a Mg·ATP-dependent phosphotransferase, respectively, both modifying the …

Characteristics Of Cover-Collapse Sinkholes In Kentucky, James C. Currens

Report of Investigations--KGS

Sudden collapse of unconsolidated earth materials over soluble bedrock, known as cover collapse, damages buildings, roads, utility lines, and farm equipment in Kentucky. It has also killed livestock, including Thoroughbred horses, and injured people. The estimated annual cost of sinkhole cover collapse in Kentucky ranges from $20 million to $84 million and is sensitive to rare but expensive events such as the 2014 National Corvette Museum collapse. The Kentucky Geological Survey began developing a catalog of case histories of cover-collapse occurrences in 1997, and receives an average of 24 reports each year. Three hundred fifty-four occurrences of cover-collapse sinkholes throughout …

A Political Companion To Frederick Douglass, Neil Roberts

Civil Rights

Frederick Douglass (1818–1895) was a prolific writer and public speaker whose impact on American literature and history has been long studied by historians and literary critics. Yet as political theorists have focused on the legacies of such notables as W. E. B. Du Bois and Booker T. Washington, Douglass's profound influence on Afro-modern and American political thought has often been undervalued. In an effort to fill this gap in the scholarship on Douglass, editor Neil Roberts and an exciting group of established and rising scholars examine the author's autobiographies, essays, speeches, and novella. Together, they illuminate his genius for analyzing …

A Broad Spectrum Racemase In Pseudomonas Putida Kt2440 Plays A Key Role In Amino Acid Catabolism, Atanas D. Radkov, Luke A. Moe

Plant and Soil Sciences Faculty Publications

The broad-spectrum amino acid racemase (Alr) of Pseudomonas putida KT2440 preferentially interconverts the L- and D-stereoisomers of Lys and Arg. Despite conservation of broad-spectrum racemases among bacteria, little is known regarding their physiological role. Here we explore potential functional roles for Alr in P. putida KT2440. We demonstrate through cellular fractionation that Alr enzymatic activity is found in the periplasm, consistent with its putative periplasm targeting sequence. Specific activity of Alr is highest during exponential growth, and this activity corresponds with an increased accumulation of D-Lys in the growth medium. An alr gene knockout strain (Δalr) was generated …

Novel Role Of Prostate Apoptosis Response-4 Tumor Suppressor In B-Cell Chronic Lymphocytic Leukemia, Mary Kathryn Mckenna, Sunil K. Noothi, Sara S. Alhakeem, Karine Z. Oben, Joseph T. Greene, Rajeswaran Mani, Kathryn L. Perry, James P. Collard, Jacqueline R. Rivas, Gerhard C. Hildebrandt, Roger A. Fleischman, Eric B. Durbin, John C. Byrd, Chi Wang, Natarajan Muthusamy, Vivek M. Rangnekar, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Prostate apoptosis response-4 (Par-4), a proapoptotic tumor suppressor protein, is downregulated in many cancers including renal cell carcinoma, glioblastoma, endometrial, and breast cancer. Par-4 induces apoptosis selectively in various types of cancer cells but not normal cells. We found that chronic lymphocytic leukemia (CLL) cells from human patients and from Eµ-Tcl1 mice constitutively express Par-4 in greater amounts than normal B-1 or B-2 cells. Interestingly, knockdown of Par-4 in human CLL-derived Mec-1 cells results in a robust increase in p21/WAF1 expression and decreased growth due to delayed G1-to-S cell-cycle transition. Lack of Par-4 also increased the expression of p21 and …

Reimagining Library Liaisons: A Liaison Competencies Mashup, Karyn Hinkle

Library Presentations

Subject specialists, collection managers, research consultants, classroom teachers, data managers, publishing advisees, and, and, and... What do the roles of academic library "liaisons" look like in 2018? What do they look like in different academic disciplines, different types of libraries? What do and could they look like in our institution?

The Association of Research Libraries (ARL) has been aiming to answer these questions through a series of Library Liaison Institutes for academic librarians to gather and discuss the role. As one of the activities presented at the ARL institutes, the "liaison competencies mashup" helps participants think creatively about what it …

Chronic Lymphocytic Leukemia-Derived Il-10 Suppresses Antitumor Immunity., Sara S Alhakeem, Mary K Mckenna, Karine Z Oben, Sunil K Noothi, Jacqueline R Rivas, Gerhard C Hildebrandt, Roger A Fleischman, Vivek M Rangnekar, Natarajan Muthusamy, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Chronic lymphocytic leukemia (CLL) patients progressively develop an immunosuppressive state. CLL patients have more plasma IL-10, an anti-inflammatory cytokine, than healthy controls. In vitro human CLL cells produce IL-10 in response to BCR cross-linking. We used the transgenic Eμ-T cell leukemia oncogene-1 (

Trace-Level Identifications Are Not Positives: The Scientifically Correct And Public Relations-Appropriate Approach To Medication Regulation, Clara Fenger, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

No abstract provided.

Illustrating A Technical Manual: Copyright And Fair Use In A Real World Professional Context, Karyn Hinkle

Library Faculty and Staff Publications

This lesson was developed for students preparing to enter professional practice who were assigned to write and/or illustrate a technical howto manual on a topic of their choice (how to put on ski boots, draw blood, use a fitness tracking app, etc.). The teaching librarian conducts a class session on finding and creating images to illustrate the manuals and teaches differences between using copyrighted and non-copyrighted images. The students work on finding images in the public domain, creating their own images, and incorporating copyrighted images via Creative Commons licenses and the principle of fair use. Librarians can teach this lesson …

Isolated Sporothrix Schenckii Monoarthritis, Aram Barbaryan, Wissam El Atrouni, Stefania Bailuc, Matthew W. Jones, Maharshi Bhakta, Khaldoun Haj Mahmoud, Aibek E. Mirrakhimov

Internal Medicine Faculty Publications

Sporothrix schenkii sensu lato is a rare cause of arthritis. Its course is indolent with lack of constitutional symptoms resulting in delayed presentation and diagnosis. It is a dimorphic fungus found ubiquitously in sphagnum moss, decaying vegetation, soil, and hay. Inoculation of dirt into the skin and soft tissues and, in rare instances, inhalation of aerosolized conidia from soil and plants can lead to infection. Subacute and chronic involvement of skin and subcutaneous tissues is the most common manifestation of sporotrichosis in immunocompetent hosts. In patients with underlying risk factors (HIV, alcoholism, diabetes mellitus, organ transplant patients, immunosuppressive medications, steroids, …

When Less Is More: Mindfulness Predicts Adaptive Affective Responding To Rejection Via Reduced Prefrontal Recruitment, Alexandra M. Martelli, David S. Chester, Kirk Warren Brown, Naomi I. Eisenberger, C. Nathan Dewall

Psychology Faculty Publications

Social rejection is a distressing and painful event that many people must cope with on a frequent basis. Mindfulness—defined here as a mental state of receptive attentiveness to internal and external stimuli as they arise, moment-to-moment—may buffer such social distress. However, little research indicates whether mindful individuals adaptively regulate the distress of rejection—or the neural mechanisms underlying this potential capacity. To fill these gaps in the literature, participants reported their trait mindfulness and then completed a social rejection paradigm (Cyberball) while undergoing functional magnetic resonance imaging. Approximately 1 hour after the rejection incident, participants reported their level of distress during …

3d Numerical Study Of Multiphase Counter-Current Flow Within A Packed Bed For Post Combustion Carbon Dioxide Capture, Li Yang, Fang Liu, Zhengchang Song, Kunlei Liu, Kozo Saito

Mechanical Engineering Faculty Publications

The hydrodynamics within counter-current flow packed beds is of vital importance to provide insight into the design and operational parameters that may impact reactor and reaction efficiencies in processes used for post combustion CO₂ capture. However, the multiphase counter-current flows in random packing used in these processes are complicated to visualize. Hence, this work aimed at developing a computational fluid dynamics (CFD) model to study more precisely the complex details of flow inside a packed bed. The simulation results clearly demonstrated the development of, and changes in, liquid distributions, wetted areas, and film thickness under various gas and liquid …

Going Beyond The "T" In "Ctc": Social Practices As Care In Community Technology Centers, David Nemer

Information Science Faculty Publications

Community technology center (CTC) is a term usually associated with facilities that provide free or affordable computer and internet access, and sometimes training, to people in underserved communities. Despite the large number of studies done on CTCs, the literature has focused primarily on the use of ICTs as the main, if not the only, activity in these centers. When it comes to addressing social concerns, the literature has often seen them as an outcome of ICT use. It does not highlight CTCs as an inherent and important social space that helps to tackle social issues. Thus, in this study, I …

The Obsolescence Of Advertising In The Information Age, Ramsi Woodcock

Law Faculty Scholarly Articles

The vast amount of product information available to consumers through online search renders most advertising obsolete as a tool for conveying product information. Advertising remains useful to firms only as a tool for persuading consumers to purchase advertised products. In the mid-twentieth century, courts applying the antitrust laws held that such persuasive advertising is anticompetitive and harmful to consumers, but the Federal Trade Commission (FTC) was unable to pursue an antitrust campaign against persuasive advertising for fear of depriving consumers of advertising’s information value. Now that the information function of most advertising is obsolete, the FTC should renew its campaign …

A Comparative Study Of Constant Power Operation Techniques For Low Inductance Machines, Damien Lawhorn, Narges Taran, Vandana Rallabandi, Dan M. Ionel

Power and Energy Institute of Kentucky Faculty Publications

In traditional electric machines, it is usually possible to achieve constant power high-speed operation by employing field weakening through the injection of a negative d-axis current component. However, in machines with low armature inductance, such as high power density permanent magnet synchronous machines, and more specifically, coreless machines, which are gaining increasing attention because of their high specific torque, the extended speed range obtained using this method is very narrow. This paper summarizes the performance characteristics of existing approaches for obtaining constant power operation and proposes two new techniques, specially applicable to coreless axial flux permanent magnet machine namely, current …

Multi-Physics Modeling For Electric And Hybrid Vehicles With In-Wheel Electric Motors, Vandana Rallabandi, Damien Lawhorn, Dan M. Ionel, Xiao Li

Power and Energy Institute of Kentucky Faculty Publications

This paper discusses multi-domain and multi-physics modeling of in-wheel electric vehicles using ANSYS Simplorer and ANSYS Maxwell. The study includes component level modeling of the vehicle, brakes, wheels, battery, traction motor, inverter, solar panels and boost converter. The traction motor used is an axial flux permanent magnet synchronous machine. In order to accommodate both the large time constants of the mechanical system and the high switching frequency power electronics, average models of the inverter and boost converter are considered. Simulation examples are provided for the University of Kentucky Gato Del Sol V car.