Digital Commons Network^™

The Oncogenic Role Of The Protein Tyrosine Phosphatase 4a3 (Ptp4a3 Or Prl-3) In T-Cell Acute Lymphoblastic Leukemia, Min Wei

Theses and Dissertations--Molecular and Cellular Biochemistry

T-cell Acute Lymphoblastic Leukemia (T-ALL) is an aggressive blood cancer. There are no immunotherapies and few molecularly targeted therapeutics available for the treatment of this malignancy. The identification and characterization of genes and pathways that drive T-ALL progression is critical for the development of new therapies for T-ALL. The protein tyrosine phosphatase PTP4A3, also known as PRL-3, has been extensively reported to play a causative role in numerous cancers, including several types of blood malignancies. However, its role in T-ALL is not well defined.

Here, we determined that the PRL-3 plays a critical role in T-ALL initiation and progression by …

Epigenetic Implications In Inorganic Arsenic-Mediated Carcinogenesis, Meredith Eckstein

Theses and Dissertations--Molecular and Cellular Biochemistry

Chronic, low dose exposure to inorganic arsenic (iAs) is a public health concern throughout the world, contributing to the development of many diseases, including lung cancer. Several mechanisms for iAs-mediated carcinogenesis have been proposed, of which the production of reactive oxygen species and formation of chromosomal aberrations are the most studied. Another equally important, yet less studied mechanism is dysregulation of epigenetic marks. “Epigenetics” refers to changes that occur on the DNA and chromatin that do not alter base pair identity, but alter compaction, expression, and regulation of specific DNA sequences. There are several types of epigenetic marks including histone …

Investigation Of Host Nanotube Parameters For Enhancing The Performance Of Nanostructured Cds-Cdte Solar Cells, Deepak Kumar

Theses and Dissertations--Electrical and Computer Engineering

Numerical simulations are performed to investigate the effects of host nanotube parameters (pore diameter and pitch for different CdS coverages) and CdTe doping density on device performance in nanowire CdS/ CdTe solar cells using SCAPS-1D. This research finds the optimum values for these parameters in order to achieve the highest efficiency. Experimentally the effect of anodization voltage and fluoride ion concentration on the pore diameter and the pitch are studied for the Titania nanotubes host. It is observed that in the range of 0.3 mL to 2 mL of ammonium fluoride content, pore diameter and the pitch of the Titania …

College Campus Outreach To Increase Chlamydia Screenings, Elizabeth Kinney

DNP Projects

A common sexual health concern around the world is sexually transmitted infections (STI). Chlamydia is the most reported sexual health problem. The highest prevalence of chlamydia rates is among ages 15-24, which includes college age students (CDC, 2019). The lack of education provided to students prior to starting college by parents, health care providers and high school sources as well as inaccurate information obtained from peers and erroneous internet sources may lead to misconceptions about STIs and consequently the avoidance of routine screening among this high-risk population (Canan & Jozkowski, 2017).

The purpose of this project was to determine if …

Neonatal Abstinence Syndrome: Effectiveness Of Targeted Umbilical Cord Drug Screening In Determining Risk Of Withdrawal, Alecia Karr

DNP Projects

Neonatal Abstinence Syndrome (NAS) is a condition in which an infant experiences withdrawal from uterine exposure to various substances such as caffeine, nicotine, amphetamines, THC, opioids, benzodiazepines, and other types of substances. Depending on the severity of the symptoms, these infants may experience a longer hospital stay and may need treatment and monitoring in the neonatal intensive care unit (NICU), resulting in an increase in healthcare costs. The objective of this project was to determine if targeted drug screening of newborns was effective in determining infants at risk of NAS based on a positive screening result. This study utilized a …

Evaluating Providers’ Perceptions On Preconception Care For Women With Sud, Corinna Hughes

DNP Projects

Background: Substance use disorders (SUDs) has become an epidemic. Women of reproductive age with SUDs have particularly complex healthcare needs including an increased risk of having an unintended pregnancy and associated sequela of negative birth outcomes (NBO). Little is known about how medication assisted treatment (MAT) providers are adequately addressing their unique needs. Identifying provider barriers and tools to address the reproductive needs of women with SUDs can optimize their health outcomes and prevent NBOs.

Purpose: The purpose of this study was to evaluate MAT Nurse Practitioners’ (NPs) knowledge and perceptions of providing preconception care to women with …

Effectiveness Of A School-Based Asthma Education Program For 8-10-Year-Old Children, Laura Hunt

DNP Projects

Background: Asthma is the most common chronic condition to affect children in the United States Five million children throughout the United States and approximately 112, 000 children in Kentucky are living with the diagnosis of asthma (CDC, 2017; CHFS, 2017). Based on current rates 1 out of 10 elementary school-age students will likely have asthma (CDC, 2019). Educating elementary-age students about asthma and symptoms of an asthma exacerbation is very important and could save a life.

Methods: A school-based asthma education program targeting children in third and fourth grades was implemented at two elementary schools in Central Kentucky. The asthma …

The Effect Of A Sun Damage Simulation Photography App On The Skin-Protective Behavior Intentions Of Women With Children Age 18 Or Younger, James Bowlds

DNP Projects

Abstract

Background: Skin cancer is the most common form of cancer diagnosed in the U.S. annually, despite being a largely preventable disease through the limitation of ultraviolet radiation exposure. Attempts to decrease its incidence have focused on appearance-based interventions. These have been effective at improving sun-protective behaviors among various subpopulations including people of color. Anecdotal observations suggest mothers more frequently utilize sun protection for their children than on themselves.

Purpose: The purpose of this project was to gain an understanding of the sun protection practices and beliefs of Caucasian and African American mothers, to assess the response to an appearance-based …

Device Related Concerns Pertaining To Primary Caregivers Of Lvad Patients, Nicole Smith, Carissa Smith

DNP Projects

Abstract

PURPOSE: The purpose of this project was to focus on caregiver education with an emphasis on caregiver concerns regarding the LVAD; additionally, evaluating the efficacy of an educational video acknowledging those device-related concerns identified by caregivers. The goal of this project is to improve caregiver knowledge and decrease the rate of device related concerns among the primary caregivers.

METHODS: This project is a single center cross-sectional, pre-post-test implementation design to determine the efficacy of device related concerns education on primary caregivers’ knowledge and concerns. A 16-point device related concerns scale was designed to appraise primary caregiver concerns. The scale …

Regulatory Networks During Seed Development, Ran Tian

Theses and Dissertations--Plant and Soil Sciences

Seeds make up approximately 70% of the human diet directly so understanding regulatory mechanisms to generate a seed and the embryo it contains is fundamentally important. The LAFL genes encode transcription factors (TF) that are critical for seed development. Three members of LAFL, LEAFY COTYLEDON2 (LEC2), ABSCISIC ACID3 (ABI3) and FUSCA3 (FUS3), are B3 domain factors that bind DNA motifs called RY motifs. While LEC2 is expressed earlier in embryo development, ABI3 and FUS3 are expressed during later development. All three of these TFs can induce embryo-specific programs after completion of germination to different extents up to and including formation …

The Balancing Act Of Cytokinin In Environmental Stress Tolerance, Sumudu Sandeepani Karunadasa

Theses and Dissertations--Plant and Soil Sciences

Cytokinin, long known as a phytohormone that regulates plant growth and development, has been recently recognized as an important regulator of stress responses. However, our current knowledge about the mechanisms by which cytokinin regulates stress responses is fragmentary, as many of the studies in this field yielded conflicting results. Most of the work described here has focused on analyses of the molecular mechanisms of cytokinin-dependent regulation of growth and development under stress conditions, with an emphasis on the role of cytokinin-dependent regulation of protein synthesis in development and stress tolerance.

One of the important contributions of this study is the …

Bimetallic Nanoparticles Integrated Membranes For Groundwater Remediation: Synthesis, Characterization And Applications, Hongyi Wan

Theses and Dissertations--Chemical and Materials Engineering

The detoxification of chlorinated organics from groundwater, such as trichloroethylene (TCE), tetrachloroethylene (PCE), polychlorinated biphenyl (PCB) and carbon tetrachloride (CTC), is a challenging area. Reductive dechlorination has been investigated using iron and iron-based nanoparticles, such as bare Fe, sulfidized Fe (S-Fe) and palladized Fe (Pd-Fe). However, issues including particle agglomeration, difficulties in recycling and particle leaching have been reported to hinder the application and wide usage of these techniques. The integration of nanoparticles and membranes can address these issues because of the large surface area, stability, and the potential for versatile functionalities. In this study, commercial polyvinylidene difluoride (PVDF) microfiltration …

Effect Of Silica Nanoconfinement Of Lipid Bilayers On Its Phase Transition And On The Colloidal Stability Of Silica Nanoparticles, Aniruddha Atul Shirodkar

Theses and Dissertations--Chemical and Materials Engineering

In this work, we incorporated 4-(N-Boc-aminometyl) phenylboronic acid (BA), at different concentrations, into 1,2-dipalmitoyl-sn-glycero-3- phosphocholine (DPPC) bilayers confined within nanopores of two different mean pore diameters of 7.4 nm and 11.7 nm of micron sized silica particles. The confinement of DPPC into nanopores resulted in the depression in the main phase transition temperatures compared to the liposomal system. The addition of BA was found to induce disruptions in the acyl chains of the lipid molecules at all concentrations of the solute. The lipid bilayer cooperativity was found to be higher in the confined systems compared to the liposomal systems despite …

Silica Nanoporous Confinement Effects On Ionic Liquid Properties For Better Design Of Small Molecule Separation, Electrochemical Devices And Drug Delivery, Yuxin He

Theses and Dissertations--Chemical and Materials Engineering

Silica nanoconfinement provides a high level of control of ionic liquids (ILs) in localizing catalysts, creating distinct environment for tuning reactivity and controlling the partition of solvents, reactants and products. Silica thin films with two different pore sizes (2.5 nm and 8 nm) were synthesized to study the effect of nanopore confinement on ionic liquids 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF₆]), and 1-butyl-3-methylimidazolium chloride ([BMIM][Cl]). Silica thin films with accessible 8 nm pore diameters were synthesized using evaporation-induced self-assembly (EISA) with Pluronic P123 as templating surfactant on a chemically neutral modified substrate. The silica films with similar orthogonal aligned mesostructured but …

Nanostructured Metal Thin Films As Components Of Composite Membranes For Separations And Catalysis, Michael J. Detisch

Theses and Dissertations--Chemical and Materials Engineering

Novel metallic thin film composite membranes are synthesized and evaluated in this work for improved separations and catalysis capabilities. Advances in technology that allow for improved membrane performance in solvent separations are desirable for low molecular weight organic separation applications such as those in pharmaceutical industries. Additionally, the introduction of catalytic materials into membrane systems allow for optimization of complex processes in a single step. By adding a nanostructured metallic thin film to its surface, a polymer membrane may be modified to exhibit these improved properties. Using magnetron sputtering, thin metal films may be deposited on commercially available membranes to …

A Framework For Heterologous Biosynthesis Of Natural Products In Mammalian Cells Via Polymer-Mediated Transfections, Logan Warriner

Theses and Dissertations--Chemical and Materials Engineering

With the promise to treat a multi-faceted list of serious inherited and acquired diseases, such as cancer, neurodegenerative and infectious diseases, and inherited genetic indications, gene therapy has continued to push the boundaries of traditional medicine since its earliest implementation. While much progress has been made, clinical success has largely remained elusive. Immunogenicity, difficulty producing commercially relevant quantities, and having a limited genetic payload still limits the ability of viruses to act as directed delivery agents for genetic material. As such, researchers have turned to cationic synthetic materials as a means of delivering nucleic acids, which can circumvent the immune …

The Development Of Temperature And Ph Responsive Hydrogels And Membranes For Selective Sorption Of Perfluoroorganics And Nanoparticle Integrated Catalytic Degradation Of Pcb, Anthony Saad

Theses and Dissertations--Chemical and Materials Engineering

The functionalization and use of responsive and catalytic polymeric membranes and materials were explored for contaminant capture and degradation. While membranes have a wide variety of uses across multiple industries, the inclusion of materials that are temperature and pH responsive in the membrane pore domain yields a wide range of applications and possibilities for water treatment. Temperature and pH responsive polymers, as well as controlled nanostructured materials, were synthesized in membrane pores for advanced adsorption-desorption and catalytic treatment of emerging organic contaminants in water. In this study, supported by the NIEHS, poly-N-isopropylacrylamide (PNIPAm) was used as a model thermo-responsive polymer, …

Heart Tissue Reconstruction By Light-Controlled Cell Adhesion, Patterning And Alignment On Decellularized Scaffolds, Cong Li

Theses and Dissertations--Chemical and Materials Engineering

There is an urgent demand for engineered tissues and organs because of extremely limited donor tissues available for transplant. The challenge of tissue rejection has motivated many researchers to try to remove all of the cells from a heart and then add back in patient matched cells. These recellularized hearts are a promising avenue towards a functional bioartifical heart that is less likely to be rejected. Decellularized extracellular matrix (ECM) scaffolds generated from animal hearts do not have the cellular components that elicit immune rejection in patient, and they also preserve the native heart tissue structure. While decellularization techniques are …

Polymeric Nanocomposite Membranes With Phosphorene Based Pore Fillers For Fouling Control, Joyner Eke

Theses and Dissertations--Chemical and Materials Engineering

Phosphorene is a two-dimensional material exfoliated from bulk phosphorus. Specifically, relevant to the field of membrane science, the band gap of phosphorene provides it with potential photocatalytic properties, which could be explored in making reactive membranes able to control the accumulation of compounds on the surface during filtration, or fouling. Another reason phosphorene is a promising candidate as a membrane material additive is due to its catalytic properties which can potentially destroy foulants on the membrane surface.

The first goal of this study was to develop an innovative and robust membrane able to control and reverse fouling with minimal changes …

Flavodoxin, The Hydrogen Atom Of Flavoproteins: A 19f Nmr Study Of Dynamics And Conformational Changes Utilizing Flavodoxin From Rhodopseudomonas Palustris, Taylor Varner

Theses and Dissertations--Chemistry

Flavodoxin is a small, highly stable protein that contain a single FMN cofactor used to transfer single electrons at low potentials. The organism Rhodopseudomonas palustris contains a long-chain flavodoxin. Long-chain flavodoxins are characterized by a 20 amino acid loop that is proposed to allow interactions with partner proteins. We plan to utilize this protein as a model to build our repertoire with protein fluorination and fluorine NMR in flavoproteins. This tool kit will then be applied to the study of a partner protein of interest that is capable of performing electron bifurcation. We have incorporated m-fluoro tyrosine into flavodoxin and …

Confederate Monuments As Badges Of Slavery, Alexander Tsesis

Kentucky Law Journal

No abstract provided.

Why Use A Hammer When A Scalpel Will Do? Suggestions For Fairer Juvenile Plea Bargaining In Kentucky, Aaron Wallace Meek

Kentucky Law Journal

No abstract provided.

Table Of Contents For Volume 109, Issue 1, Kentucky Law Journal

Kentucky Law Journal

No abstract provided.

Hyperreal: Law And The Interpretation Of Visual Media, Amy L. Landers

Kentucky Law Journal

No abstract provided.

Intentional Personality Change: Toward The Prevention Of Problem Drinking, Elizabeth N. Riley

Theses and Dissertations--Psychology

Personality stability across the lifespan has been well documented, but within that overall stability there is also evidence of meaningful change. There is both theoretical and empirical evidence to suggest that personality change can occur at the volition of the individual, through behavioral processes. The current study tested whether an emotion modulation intervention that promoted behavior change could be applied to reduce a related, high-risk personality trait (negative urgency) and a high-risk behavior (heavy alcohol consumption) using a three-week long, mixed laboratory design. Participants (n=23) were a sample of heavy drinking but otherwise healthy volunteers who were randomly assigned to …

Examining The Impact Of Social Media Use On Body Dissatisfaction And Eating Disorder Symptomatology Among Adolescents, Ilyssa P. Salomon

Theses and Dissertations--Psychology

Media exposure is often cited as a causal factor in the development of body dissatisfaction, or negative thoughts and feelings toward the body (Grogan, 2017; Thompson, Heinberg, Atlabe, & Tantleff-Dunn, 1999). While eating disorders most commonly emerge during late adolescence (18-21 years), risk factors that predict the later onset of eating disorders emerge much earlier and escalate during adolescence (13-16 years; Hudson, Hiripi, Pope, & Kessler, 2007; Rhode, Stice, & Marti, 2016). Overall, links between exposure to traditional forms of mainstream media (e.g. television and magazines), body dissatisfaction, and eating disorder symptomatology are well- established in the literature, with robust …

Ethanol Sustains Phosphorylated Tau Protein Immunofluorescence In The Cultured Neonatal Rat Hippocampus: Implications For Fetal Alcohol Spectrum Disorders, Caleb Seth Bailey

Theses and Dissertations--Psychology

Fetal Alcohol Spectrum Disorders (FASDs) are comprised of developmental, behavioral, and cognitive abnormalities caused by prenatal alcohol exposure, affecting an estimated 2%-5% of childrenand costing up to $4 billion annually in the United States alone. Although some behavioral therapies can help, the biochemical mechanisms that underpin FASDs need further elucidation for development of more efficacious therapeutics. The tau protein modulates cytoskeletal structure in neurons, and thereby plays an integral role in proper development and function of the central nervous system, but its function is altered by its phosphorylation state, such that increased phosphorylation reduces tau protein function. The tau protein …

Study Of Cell Charging Effects For The Neutron Electric Dipole Moment Experiment At Oak Ridge National Laboratory, Mark Broering

Theses and Dissertations--Physics and Astronomy

The neutron electric dipole moment (nEDM) collaboration at the Spallation Neutron Source plans to use ultra-cold neutrons in superfluid helium to improve the nEDM limit by about two orders of magnitude. In this apparatus, neutrons are stored in poly(methyl methacrylate), PMMA, cells located in a strong, stable electric field. This electric field is produced by high voltage electrodes located outside of the neutron cells. Several sources generate charged particles inside the neutron cells. The electric field pulls these charges farther apart, attracting each to the oppositely charged electrode. As the charges build up on the cells walls, they create an …

Restructuring Work “The Chattanooga Way”: Urban Revitalization, Contingent Labor, And Trying To Get By In Tennessee, Mauri Systo

Theses and Dissertations--Anthropology

In Chattanooga, TN, the construction of a fiber optic telecommunications network has led to a tech-based revitalization strategy, and the promotion of entrepreneurial and technical positions within the Downtown. This dissertation questions what revitalization, the “Chattanooga Way,” means to differently situated residents of Chattanooga, TN, and how those differences in interpretation are related to lived experiences of economic inequality. Powerful local discourses, like the Chattanooga Way policy model and its accompanying “origin myth” of Chattanooga’s development often conceal disparities between grass-roots, public, and private sector notions of economic revitalization. Through the projection of a tech-based economic future, Chattanooga has created …

Carceral Extractivism, Livelihood Strategies, And “Acting Right” In The U.S. South, Edward L. Bullock

Theses and Dissertations--Anthropology

Mass incarceration and its effects are well documented and carceral privatization is hotly contested on moral and economic grounds. This dissertation examines the local effects of carceral privatization in the U.S. south in historical context. Tallulah is a small, rural predominately African American town in northeastern Louisiana that endures high rates of poverty, unemployment, and low educational attainment. It also hosts four private prisons operated by LaSalle Corrections, LLC. Two primary and overlapping questions guide the research. 1) How has an history of carceral entrepreneurship and mass incarceration impacted the way persons and communities create livelihoods and imagine futures, and …