Digital Commons Network^™

Establishing Exclusive Breastfeeding And The Influence Of Hospital Support, Martha Monroe

Theses and Dissertations--Nursing

Maternal-child health authorities recommend that infants breastfeed exclusively for the first six months of life. Currently, 25% of infants in the United States do so. Breastfeeding specific care that mothers receive from their postpartum nurses and lactation specialists is integral to successfully initiating exclusive breastfeeding. Due to the time sensitive nature of establishing milk supply, interrupting the process of lactogenesis with supplementation puts infants at high risk for early termination of breastfeeding. The purpose of this dissertation is to examine the relationship of breastfeeding care received during the postpartum hospital stay, defined as the first 48-72 hours of life, and …

Relationship Between Initial Prescription Opioid Exposure Length And Future Opioid Use Disorder Diagnosis In Opioid Naive Adolescents, Eric Lindahl

Theses and Dissertations--Pharmacy

Objectives: The long-term risks associated with the use of short-term prescription opioids in opioid naïve adolescents in not well characterized. The purpose of this study was to explore the potential association between the days’ supply of the initial prescription opioid exposure and the rates of diagnosed OUD in the subsequent 3-year period. Methods: We conducted a retrospective cohort study using a nationwide database of commercially-insured adolescents aged 12-17 at the time of the index opioid fill. A multivariable Cox Proportional Hazard regression model was developed to analyze the association of interest while accounting for known risk factors for the development …

Combination Of Investigational Cell-Based Therapy And Deep Brain Stimulation To Alter The Progression Of Parkinson’S Disease, Nader El Seblani

Theses and Dissertations--Pharmacy

Parkinson’s disease (PD) is the second most common neurodegenerative disorder and the motor symptoms are caused by progressive loss of midbrain dopamine neurons. There is no current treatment that can slow or reverse PD. Our current “DBS-Plus” clinical trial (NCT02369003) features the implantation in vivo of autologous Schwann cells (SCs) derived from a patient’s sural nerve into the substantia nigra pars compacta (SNpc) in combination with Deep Brain Stimulation (DBS) therapy for treating patients with advanced PD.

The central hypothesis of our research is that transdifferentiated SCs within conditioned nerve tissue will deliver pro-regenerative factors to enhance the survival of …

Correlating The Physicochemical Properties Of Magnesium Stearate With Tablet Dissolution And Lubrication, Julie L. Calahan

Theses and Dissertations--Pharmacy

Magnesium stearate (MgSt) is the most commonly used pharmaceutical excipient and is present in over half the tablet formulations on the market. In spite of its popularity as an effective lubricant, it has been repeatedly recognized that there is significant variability between MgSt samples, which can cause inconsistent lubrication between batches of MgSt. The hypothesis of this research is that the batch-to-batch variability in tablet lubrication and dissolution observed in tablet formulations containing different MgSt samples can be correlated with differences in MgSt physicochemical properties (fatty acid salt composition, crystal hydrate form, particle size and surface area). Developing correlations between …

National Guard Members With Suicide Ideation: The Impact Of Stigma, Mental Health, And Trauma History On Treatment-Seeking Outcomes, Amy Brown

Theses and Dissertations--Social Work

Background. Veterans are overrepresented among suicide deaths in the United States, representing 20% of annual suicide deaths but only about 1% of the U.S. population (Department of Veteran Affairs, 2010). Of all military suicide deaths in 2016, one-third (33.4%) were in the National Guard or Reserves (Department of Defense, 2018). Previous suicidal ideation or behavior (SIB) has been shown to increase risk of subsequent ideation or behavior in both active duty and veteran samples (Bryan, Rudd, & Wertenberger, 2016; Hazlett et al., 2016) but studies of SIB specific to National Guard service members were not found.

Access and Barriers to …

Electrophoresis In Heterogeneous Hydrogels And Applications In Surface Patterning, Ning Ge

Theses and Dissertations--Mechanical and Aerospace Engineering

The creation of chemical micropatterns on surfaces makes it possible to add unique chemical functionality to surfaces, modifying properties such as wettability, or even adding the ability to selectively bind other molecules. The creation of biochemical surface patterning in particular is useful in a variety of fields including tissue engineering and highthroughput drug screening. There are many existing surface patterning techniques which focus on precise control over the patterned geometry, even down to submicron scale features, but they do not allow local control over chemical concentration. So the results are high resolution patterns with binary concentration. There are also existing …

Safety Concepts For Every Ride: A Statistical Ensemble Simulation To Mitigate Rotational Falls In Eventing Cross Country, Shannon Wood

Theses and Dissertations--Mechanical and Aerospace Engineering

Rotational falls are the leading cause of death and serious injury in the equestrian sport of eventing. Previous studies to develop safety devices used physical models representing one or at most several physical situations leading to different designs and no common understanding. In this thesis, a statistical ensemble model is developed and applied to generate and evaluate 10,000 different situations that might potentially lead to rotational falls. For accurate statistical representation of the horse and rider inertia distributions, measurements of over 400 training or competing horses and riders were recorded and incorporated. Video was recorded of 218 total competitors approaching …

Development Of Micro-Scale High Aspect Ratio Patterned Features With Electroless Nickel Plating, Lorli Smith

Theses and Dissertations--Mechanical and Aerospace Engineering

This thesis describes a novel method designed to pattern high aspect ratio metallic microscale features using a modified photolithography and electroless nickel plating process. This method utilizes modified photolithography techniques to create a polymer mold that is used to control the location of metal deposition on substrate during electroless nickel plating. In order to generate high aspect ratio mold features, a multiple spin-step process was developed to deposit thick layers of SU-8 photoresist, and inclined lithography was also used to generate tapered sidewalls that could help aid mold removal after plating. Results from electroplating experiments were evaluated using a Zygo …

Development Of Universal Solver For High Enthalpy Flows Through Ablative Materials, Umran Duzel

Theses and Dissertations--Mechanical and Aerospace Engineering

Atmospheric entry occurs at very high speeds which produces high temperature around the vehicle. Entry vehicles are thus equipped with Thermal Protection Systems which are usually made of ablative materials. This dissertation presents a new solver that models the atmospheric entry environment and the thermal protection systems. In this approach, both the external flow and the porous heat shield are solved using the same computational domain. The new solver uses the Volume Averaged Navier-Stokes Equations adapted for hypersonic non-equilibrium flow, and is thus valid for both domains. The code is verified using analytical problems, set of benchmarks and also a …

Transcriptomic And Cellular Response To Mechanical Overload And The Underlying Role Of Macrophages In Extracellular Matrix Remodeling, Bailey D. Peck

Theses and Dissertations--Rehabilitation Sciences

The extracellular matrix (ECM) in skeletal muscle plays an integral role in tissue development, structural support, and force transmission. Upon mechanical loading, including resistance exercise, which alter muscle fiber contractile activity, size, orientation and connectivity, remodeling processes must occur that involve both ECM deposition and degradation. ECM remodeling involves many cell types in muscle, but the focus of our research was directed towards macrophages, which participate in the early immune response to damage and loading. We have consistently demonstrated a significant increase in skeletal muscle macrophage abundance using pan macrophage markers (CD11b/CD68) and anti-inflammatory markers (CD206/CD163) following exercise training in …

Functional Impact Of Angiotensinogen Specific Domains On Angiotensin Ii-Mediated Functions, Chia-Hua Wu

Theses and Dissertations--Pharmacology and Nutritional Sciences

Angiotensinogen (AGT) is the only substrate for all angiotensin peptides in the renin angiotensin system (RAS). Cleavage of AGT by renin is a rate-limiting step of angiotensin peptides productions in the RAS and regulates angiotensin peptides-associated pathophysiological functions. Only ten N-terminal residues are cleaved by renin and the functions of remaining part of AGT protein, which is called des(angiotensin I)AGT, remain unclear. Despite of pivotal roles of AGT in the RAS, studies related to how AGT is metabolized and how des(angiotensin I)AGT regulates AGT functions or AngII-mediated functions are limited.

Renin cleavage of AGT shows species specificity. It has been …

Involvement Of The Renin Angiotensin System In Marfan Syndrome Associated Thoracic Aortic Aneurysms, Jeff Zheying Chen

Theses and Dissertations--Physiology

Thoracic aortic aneurysms (TAAs) are clinically-silent dilations of the aorta which greatly increase the risk of aortic rupture, a condition with 50-90% mortality. Marfan syndrome (MFS) is caused by mutations in fibrillin-1 (FBN1) and is associated with TAAs. Due to an absence of validated and effective pharmacologic therapies to prevent or reverse TAA, most MFS patients require surgical aortic repair. Understanding MFS associated TAA pathogenesis would direct development of new pharmacologic therapies. Previous research has implicated the renin angiotensin system in TAA. In both males and females, angiotensinogen (AGT) is cleaved serially to generate the main effector peptide angiotensin II …

Remodeling In The Actin Core Of The Auditory Hair Cell Stereocilia As A Novel Component Of Temporary Noise-Induced Hearing Loss, Jonathan Michael Grossheim

Theses and Dissertations--Physiology

The rigid, paracrystalline actin core of auditory hair cell stereocilia is extremely stable and after initial formation must persist for the life of the cell to preserve hearing in mammals. In healthy hair cells, turnover of actin molecules occurs only in a small region near the tips of stereocilia, while the actin filaments of the shaft are stable. For decades damage to the actin core of stereocilia from acoustic trauma has only been attributed to cases of permanent noise-induced hearing loss. Here, we show that repairable actin core damage occurs in temporary noise-induced hearing loss from moderate acoustic trauma.

We …

Pathogenicity, Toxigenic Potential, And Genomics Of Fusarium Graminearum And F. Meridionale Causing Ear And Stalk Rot Of Maize, Franklin Jackson Machado

Theses and Dissertations--Plant Pathology

Gibberella ear (GER) and stalk rot (GSR) diseases of maize in Brazil are caused mainly by Fusarium meridionale, a species belonging to the Fusarium graminearum species complex (FGSC). Another species within this complex, F. graminearum sensu stricto (hereafter F. graminearum), is second in importance on maize, but is the most common species found causing Fusarium Head Blight disease of wheat in Brazil. The latter species is the predominant cause of GER and GSR in North America, where F. meridionale has not been found thus far. In this dissertation I undertook a comparative analysis of pathogenic, saprophytic, toxigenic and …

Evaluation Of Rhodococcus Equi Susceptibility To Silver Nanoparticle Antimicrobials, Elizabeth Boudaher

Theses and Dissertations--Veterinary Science

Rhodococcus equi is a significant cause of pneumonia in foals and immunocompromised humans. Antimicrobial resistance among R. equi isolates has developed as a consequence of inappropriate stewardship and bacterial evolution, leading to an increased rate of treatment failures that typically result in foal fatality. In the current study, we evaluated the efficacy of antimicrobial silver nanoparticle (AgNP) complexes in controlling R. equi growth. Previous studies characterizing AgNP-induced antibacterial effects in other Gram-positive pathogens led us to hypothesize that silver nanoparticle antimicrobials impact R. equi viability and intracellular replication. We therefore investigated the effect of silver nanoparticle complexes on R. equi …

Diet Of A Recently Reintroduced River Otter (Lontra Canadensis) Population In Taos County, New Mexico, Gabriela Alexandra Wolf-Gonzalez

Theses and Dissertations--Forestry and Natural Resources

North American river otters (Lontra canadensis), native to every U.S. state and Canada, experienced extensive population decreases and range reduction until the mid-20th century as a result of overexploitation and habitat loss during European colonization. The last known river otter in New Mexico was killed on the Gila River in 1953, although unverified reports continued thru 2008. After a nearly 60-year absence from New Mexico, 33 adult river otters were reintroduced to the Rio Pueblo de Taos in the northern part of the state between 2008-2010; however, they were not subsequently monitored or studied. I characterized diet of …

Race-Related Stress, Resiliency, And Relationship Quality In Black Couples, Melinda Murdock

Theses and Dissertations--Family Sciences

Scholars have historically explained Black marriage patterns of instability and dissolution based on White middle-class models that ignore cultural factors and maintain a narrative of dysfunction. The current study examines resilience in Black couples by exploring mediation effects of attribution and dyadic coping processes on race-related stress and relationship quality. The present study used individual data from 131 middle-income Black couples residing in the South, who self-reported on stress, coping, and relationship quality via online survey. Dyadic Coping was predicted to mediate the relationship between Race-related Stress, Attribution, and Relationship quality. Results indicated that individuals who experienced greater stress from …

Does Parental Stress Influence Parent-Child Sexual Communication?, Kirstie Otto

Theses and Dissertations--Family Sciences

This study shined a light on the impact of parent’s sexual attitudes and parental stress has on parent-child sexual communication. A sample of 203 parents between the ages of 30 and 60 were recruited to complete an online survey about their levels of parental stress, their sexual attitudes, and how often they discuss sexual communication with their adolescents. Beliefs that the responsibility of birth control should be shared between all parties involved, set the foundation that there will be higher levels of parent-child sexual communication. These findings inform therapists about the importance of creating a safe place where families can …

Stress And Cancer Treatment: How A Family’S Adaptability And Cohesion Affects Financial Stress, Emily Marie Johnson

Theses and Dissertations--Family Sciences

According to the American Cancer Society (2019), it is estimated that 1,762,450 new cancer diagnoses occurred in 2019 in the United States. Currently, cancer remains one of the leading causes of death worldwide (American Cancer Society, 2019). As cancer affects the family, roles within the family will shift (e.g., transitioning to a caregiving relationship), causing each family member to adapt. With the costs of cancer steadily increasing, this potentially leaves a devastating impact on the family. When considering family function in terms of Family Systems, John Rolland created the Family Systems Illness Model to explain how families adapt to an …

An Educational Pilot Intervention To Increase Environmental Health Literacy Among Rural Kentucky Youth Attending Summer Camp, Ashley Rose Mattingly

Theses and Dissertations--Nutrition and Food Systems

Risk factors, including exposure to environmental pollutants, poor dietary and overall health outcomes, and low environmental health literacy (EHL), place the rural Appalachian youth population at increased risk for chronic diseases. Implementation of nutrition and pollution curriculum into two week-long summer day camps for youth ages 5-12 years that focus around healthy lifestyles and environmental stewardship has the potential to increase health protective action in this community. This research utilized qualitative interviews of 30 Kids on the Creek Camp attendees to determine the efficacy of the implementation of an active learning lesson to increase EHL by explaining the link between …

The Development Of A Couple Observational Coding System For Computer-Mediated Communication, Martha S. Rackets

Theses and Dissertations--Family Sciences

Many romantic couples integrate text and computer-mediated communication (CMC) into their relationship dynamics, both for general relationship maintenance and for complex dynamics such as problem solving and conflict. Romantic couple dynamics are interactional, dynamic, and sequenced in nature, and a common method for studying interactions of this nature is observational analyses. However, no behavioral or observational coding systems exist that are able to capture text-based transactional couple communication. The main purpose of this dissertation was to develop an observational coding system that can be used to assess sequenced computer- mediated, text-based communication that takes place between romantic partners. This process …

Utilizing A Formative Program Evaluation Approach And An Application Of Grounded Theory To Assess A Novel Harm Reduction Program, Dia Obonyo

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Formative program evaluation is conducted during development and early implementation to ensure a program is feasible, appropriate, and acceptable. The Harm Reduction Initiative (HRI), a program of KIRP, the Kentucky AIDS Drug Assistance (KADAP) Income Reinvestment Program, seeks to address some of the underlying causes of infectious disease vulnerability resulting from drug use by focusing on infectious disease testing and linkage to care among high risk individuals. The primary objective of the evaluation was to achieve the following aims: 1) characterize the need for the program, 2) clarify the theory and logic of the program, 3) inform implementation, and 4) …

The Bluegrass Reentry Council Strategic Plan, Sydney Geiger

MPA/MPP/MPFM Capstone Projects

The overcrowding of jails and prisons and high recidivism rates are issues America is facing nationwide. Successful reentry into society following incarceration is a solution to this crisis. In response, in 2011, the Kentucky legislature passed House Bill 463 which mandated reentry councils across the state. The purpose of the councils are to address the current issues facing citizens returning from the justice system, develop a network of resources and service providers within their home area, and communicate and advocate for returning citizens by communicating issues to the community and their leaders.1 This analysis will develop a strategic plan for …

Performance Evaluation And Community Needs Of Spay Our Strays Organization, Elizabeth Adams

MPA/MPP/MPFM Capstone Projects

Free-roaming cat populations have been linked to public health concerns and negative impacts on animal shelter resources. Trap-neuter-return has gained popularity in the last few decades as a humane and effective approach to decrease the population of community cats and address these concerns. Spay Our Strays advocates for trap-neuter-return practices in Lexington, Kentucky, by providing services to the local community and surrounding counties. Strategic planning techniques and comparative analysis are used to develop recommended strategic actions the organization can use to improve their impact. Findings showed a need to invest in board development, diversify funding sources, focus on community-level targeting, …

Congressional Appropriations And Earmarks An Analysis Of The Economic And Political Effects, Paul Anthony Allen

MPA/MPP/MPFM Capstone Projects

The effects of political characteristics on the Congressional appropriations process have remained a subject of debate in recent years. Congressionally directed spending influenced by these effects are defined as earmarks. To counter the practice of earmarking, Congress implemented multiple reforms and rules to curb the influence of partisan agendas. Total federal appropriations by year and state from 2002 – 2018 were aggregated to test the significance of the economic and political effects. This model utilized a time series fixed effects regression to determine the results that suggest select political characteristics of U.S. Senators remained significant in the distribution of federal …

Inclusive Recreation In Kentucky State Parks: A Survey Of Ada Compliance And Transcendent Programing, Jason Thomas

MPA/MPP/MPFM Capstone Projects

Under the Americans with Disabilities Act of 1990, disabled, or differently abled, members of American communities are entitled to receive identical, or equivalent, treatment and admission to public services, without discrimination based solely on their disability. Therefore, it is in violation of this act to not accommodate public spaces and facilities for these individuals, with the exception of cases where doing so to an existing facility would demonstrably, and with burden of proof on the agency, “result in a fundamental alteration in the nature of a program or activity or in undue financial and administrative burdens” (28 CFR § 39.150). …

A Cost Benefit Analysis Of Blupass: The University Of Kentucky's Fare Free Transit Partnership With Lextran, Sandra Broadus

MPA/MPP/MPFM Capstone Projects

Parking is an omnipresent headache for urban-based universities across the United States. The University of Kentucky took a distinctive approach to shifting commuter mode share away from single-occupancy vehicles in 2015 by initiating a fare-free partnership with the local transit authority. This study aims to determine the relative success of this program by weighing the costs and benefits for the University over the first four years of implementation. In order to offset the limited supply of parking available for personally owned vehicles, the University of Kentucky (UK) launched BluPass, a fare-free transit initiative that aimed to significantly increase the number …

Extracting Secondary Metabolites From Bacterial Endophytes To Suppress Or Promote Sweet Sorghum And Weeds, Mohammad R. Alsabri

Theses and Dissertations--Plant and Soil Sciences

Endophytic bacteria are ubiquitous in agricultural settings and have the potential to drastically affect plant growth both positively and negatively. These complex relationships play a key role in plant nutrition and are therefore of great interest to the agricultural community and beyond. With my dissertation work, I have had the opportunity to examine the relationship between endophytic bacteria and plants in various ways. The first part of my dissertation investigated how the microbial diversity found in soil affects the below- and above-ground traits of Sorghum bicolor. In the second section, I focused on a particular strain of bacteria that …

Landslides Across The Usa: Occurrence, Susceptibility, And Data Limitations, Benjamin B. Mirus, Eric S. Jones, Rex L. Baum, Jonathan W. Godt, Stephen Slaughter, Matthew M. Crawford, Jeremy Lancaster, Thomas Stanley, Dalia B. Kirschbaum, William J. Burns, Robert G. Schmitt, Kassandra O. Lindsey, Kevin M. Mccoy

Faculty, Staff, and Affiliated Publications--KGS

Detailed information about landslide occurrence is the foundation for advancing process understanding, susceptibility mapping, and risk reduction. Despite the recent revolution in digital elevation data and remote sensing technologies, landslide mapping remains resource intensive. Consequently, a modern, comprehensive map of landslide occurrence across the United States (USA) has not been compiled. As a first step toward this goal, we present a national-scale compilation of existing, publicly available landslide inventories. This geodatabase can be downloaded in its entirety or viewed through an online, searchable map, with parsimonious attributes and direct links to the contributing sources with additional details. The mapped spatial …

Characterization And Enzyme Engineering Of Laccases Towards Lignin Valorization In Aqueous Ionic Liquids, Joseph Stevens

Theses and Dissertations--Biosystems and Agricultural Engineering

Lignin is one of the most abundant polymers found in nature, making up 15 – 40% of the weight of terrestrial biomass. Due to the structural and monomeric heterogeneity of lignin, it is recalcitrant thermochemical and biological valorization methods. Converting lignin to value-added products via sustainable and cost-effective pathways will reduce waste and add value to future cellulosic biorefineries. Biological methods for lignin valorization (e.g. lignin degrading enzymes or microbes) is limited by low lignin solubility in biocompatible solvents, resulting in low product yield. Recent reports on biocatalysts for lignin valorization have focused on the lignolytic multicopper oxidase laccase, …