Digital Commons Network^™

The Measurement Of The Impact Of Well-Being Resources On Moral Distress And Burnout Following The Covid-19 Pandemic In Front-Line Nurse Leaders, Benjamin Hughes

DNP Projects

Background: Due to the COVID-19 pandemic, there is significant burnout among front-line nursing staff. Little literature has been published about the measurement of burnout among front-line nursing leaders, such as nurse managers and directors, nor the interventions used to help with this ever-growing issue among this group. Since the pandemic began, there have been many resources identified to help front-line staff members with burnout, but none specifically looking at what has worked or been utilized by front-line nursing leaders.

Purpose: The purpose of this project was to describe what well-being resources were identified as being effective in decreasing moral distress …

The Effect Of Relational Aggression Training On New Emergency Department Nurses, Mary Wethington

DNP Projects

BACKGROUND: Relational aggression (RA) in nursing is widespread. One group often at risk for exposure to RA is the newly licensed nurse. RA can lead to a negative work environment which can cause poor patient outcomes and high nurse turnover.

PURPOSE: The purpose of this study was to evaluate the effects of an educational program on RA in the emergency department with newly licensed registered nurses. This was conducted using one pre and two post surveys. The surveys evaluated the nurses’ knowledge of RA self-awareness before and six weeks after an educational session.

METHODS: This was a single-site, single-unit pretest …

Activating The Patient: A Nurse Led Coaching Intervention To Engage Health Information Seeking Behaviors Using The Ideal Discharge Framework And Get Well Network, Korinne Callihan

DNP Projects

Background: Cardiovascular (CV) patients are often overwhelmed by the unexpected emergence of the health condition, volume of knowledge for self-care mastery and acceptance of the responsibility for self-care upon care transition to the home setting. Low health literacy levels have been linked to poor outcomes in CV patients, requiring investigation into appropriate methods for patient education. The Get Well Network (GWN), an interactive digital patient education care plan at UK HealthCare (UKHC), is underutilized in this patient population.

Purpose: the purpose of this study is to assess the effect of a nurse-to-patient coaching intervention using the IDEAL discharge framework from …

Effect Of Anticipatory Guidance On Parental Knowledge And Anxiety Prior To Discharge Of Infant From The Neonatal Intensive Care Unit To Home, Stephanie Kelty

DNP Projects

Background: Lack of adequate parental education and discharge readiness from the Neonatal Intensive Care Unit (NICU) increases the risk for poor infant outcomes, hospital readmissions, postpartum depression, parental anxiety, and post-traumatic stress disorder. One component of parental readiness at discharge is receiving anticipatory guidance from a trusted healthcare provider prior to discharge.

Purpose/Objectives: This study aimed to evaluate the effectiveness of provider-led anticipatory guidance to enhance parents’ knowledge regarding high-risk infant behaviors and decrease anxiety related to caring for their high-risk infant after NICU discharge. Additionally, this study aimed to assess parental opinions about the value of anticipatory guidance sessions. …

Regulatory Mechanisms In Arabidopsis Embryogenesis, Sanjay Joshi

Theses and Dissertations--Plant and Soil Sciences

Plants have amazing regeneration properties with single somatic cells, or groups of cells able to give rise to fully formed plants. One means of regeneration is Somatic Embryogenesis (SE), by which an embryonic structure is formed that “converts” into a plantlet. Somatic embryogenesis has been used as a model for zygotic processes that are buried within layers of maternal tissues. Understanding mechanisms of somatic embryo induction and development are important as a more accessible model for seed development. We rely on seed development not only for most of our caloric intake, but also as a delivery system for engineered crops …

The Impact Of Plant Secondary Metabolites On Auxin And Cytokinin Signaling, Timothy E. Shull

Theses and Dissertations--Plant and Soil Sciences

Secondary metabolites are a broad class of specialized compounds that mediate plant-environment interactions and mitigate stress. It is increasingly clear that many phenylalanine-derived secondary metabolites are nearly indispensable for plant survival and that plants adjust their growth according to their secondary metabolic outputs. Consequently, many phenylalanine-derived secondary metabolites have influence over hormone activity. For instance, multiple phenylpropanoid intermediates and catecholamines alter the sensitivity of plants to the central hormone auxin, which in concert with cytokinin directs most aspects of plant growth and development. This dissertation reviews previous research on the influence of phenylpropanoid intermediates and catecholamines on plants, with a …

An Evaluation Of Eragrostis Tef For Future Cultivar Development, Eric Wayne Luteyn

Theses and Dissertations--Plant and Soil Sciences

The many benefits of utilizing teff (Eragrostis tef) as small grain is well documented in journal publications. Over time the domestication of this species has become essential to the diets of the local population and the livestock living in the African highlands. Current research points to a high diversity of trait characteristics found in many teff populations and the potential utilization of these traits to increase desirable characteristics in both grain and forage varieties. With the overarching goal of adding to what is already known about teff, this study evaluates the different traits that are of interest to …

Microscale Contact And Friction Of Low Modulus, Lightly Crosslinked Polydimethylsiloxane, Justin Glover

Theses and Dissertations--Chemical and Materials Engineering

Friction and adhesion of soft materials are important for pressure sensitive adhesives, biomaterials, and soft robotics; however, the behavior on the microscale is not fully understood. When two objects come into contact, their interactions are usually mediated by small contact points due to surface roughness. At the microscale size, surface forces can deform soft materials to minimize energy by increasing the contact area, which is balanced by the elastic deformation of the polymer network. However, for soft, crosslinked materials with a modulus below ~100 kPa, it is challenging to predict the behavior with prior contact and friction models. Additionally, lightly …

Versatile Functionalization Strategy For The Capture Of Antibodies Using Silica Nanoparticles, Sayma Afrin

Theses and Dissertations--Chemical and Materials Engineering

In this work, the synthesis of protein-functionalized nonporous silica nanoparticles and their application to capture antibodies was demonstrated. Silica nanoparticles were chosen as they offered particle and pore size tunability, low toxicity, thermal stability, ease of synthesis and ease of surface functionalization. A biotin-streptavidin-biotin bridge was constructed on the surface of the particles and used to attach biotinylated Protein A/G (PAG), which has high affinity for antibodies. The biotin-streptavidin-biotin bridge is hypothesized to orient the PAG in a favorable manner for efficient antibody capture. Nonporous silica nanoparticles (approximately 270 nm in diameter) were synthesized via the Stöber method. Following amine …

Development Of Soluble Bioactive Poly(Beta Amino Esters) (Pβaes) For Post-Synthesis Processing, Kelley Wiegman

Theses and Dissertations--Chemical and Materials Engineering

Polymers have proven to be a viable platform to facilitate the controlled and targeted release for a variety of therapeutic agents. Polymeric prodrugs are a growing area of drug delivery in which active pharmaceutical ingredients are covalently linked to a polymeric form in an effort to enhance stability, delivery, and pharmacology. One naturally occurring class of active ingredients, known as polyphenols, can potentially offer a vast array of beneficial therapeutic properties. However, the therapeutic efficacy of these compounds is severely limited due to poor solubility and bioavailability as well as an oxidative and thermal instability. To improve the efficacy of …

Black Youth Are Suffering In Silence: Advancing Social Work Education To Better Address The Mental Health Of African American Youth., Alicia Dawson

DSW Capstone Projects

African American youth’s rates of major depression, generalized anxiety disorder, post-traumatic stress disorder, and suicide have increased in the last three years due to COVID-19 and national social injustices. African American students who seek help at school are less likely to receive adequate treatment from school social workers. Mental health care for African American students should be available, and those services must be effective. African American youth continue to suffer in silence when school social workers lack African-centered conceptual teaching and therapeutic skills. Current social work education curriculums contribute to 'Whiteness,' which influences future social workers to carry biases and …

Nanomaterial Synthesis And Real Time Investigation Of Thermal Effects On Nanomaterial And Nano-Interfaces For Real World Applications, Rose H. Pham

Theses and Dissertations--Chemistry

As interest in nanomaterials and nanotechnology continues to grow, so does the need for more efficient and economical synthesis methods to keep up with the demand. The applications for nanomaterials are seemingly endless as they have functions in energy, biomedical, environmental, and many more. Working to develop different morphologies and sizes of nanomaterials will further help expand its utility. With the use of advanced characterization techniques such as in situ transmission electron microscopy (TEM), real time studies on the effects of external forces on nanomaterials are possible under controlled environments. This will give insight on how nanomaterials will perform in …

Mechanism Of Antibiotic Permeability Through The Gram-Negative Bacterial Envelope, Olaniyi Alegun

Theses and Dissertations--Chemistry

The outer membrane of Gram-negative bacteria (GN) makes them distinct among superbugs that are associated with the development of antibiotic resistance. The outer membrane, and inner membrane, separated by the periplasm, form a double-membrane barrier to the entry of antibiotics into the cell. Several studies have been conducted to examine the role of outer membrane modifications such as porins, lipopolysaccharides, and efflux pumps on antibiotic resistance. However, there is a paucity of knowledge on how antibiotics behave in the periplasm, to gain access into their target region. My thesis focuses on understanding the mechanism of antibiotic permeability through the cellular …

Antibiotic Permeation In Gram-Negative Bacteria And Contribution Of Inflammasome Activation And Pyroptosis In Pathogenesis Of Salmonella Systemic Infection, Ankit Pandeya

Theses and Dissertations--Chemistry

Antibiotic resistance is one of the major global issues in the field of public health and medicine. Good antibiotic candidates need to be selectively toxic, inhibit cellular target, and effectively penetrate and accumulate in bacterial cells. The last factor is a formidable barrier in the development of antimicrobials effective in Gram-negative bacteria, due to the presence of two layers of cell envelope. The first half of my thesis focuses on understanding the permeation of small molecules through this formidable cell envelope, distribution inside the cell of Gram-negative bacteria, and design of novel methods to make small molecules effectively cross the …

Nicotine And Cotinine: Microbial Modulators Of The Immune Response, Jourdan Lakes

Theses and Dissertations--Chemistry

The consumption of tobacco products is a known contributor to a variety of health conditions and disease pathologies including cardiovascular and lung diseases. In addition to these well-known pathological effects of tobacco use, it has been established recently that tobacco consumption as well as nicotine consumption alone results in distinct, dysbiotic perturbations to the enteric microflora of the gastrointestinal tract. Previous research into the intestinal microbiome has demonstrated that these microbes are active contributors to the maintenance of a homeostatic and tolerogenic environment within the host. In fact, the presence of our enteric microflora is necessary for the proper development …

Succinylated Polyethylenimine Gene Delivery Agents For Enhanced Transfection Efficacy, Md. Nasir Uddin

Theses and Dissertations--Chemistry

Gene therapy aims to treat patients by altering or controlling gene expression. Today, most clinical approaches are viral-based due to their inherent gene delivery activity. However, there is still a significant interest in nonviral alternatives for gene delivery, particularly synthetic lipids and polymers, that do not suffer the immunogenicity, high cost, or mutagenesis concerns of viral vectors. Polymeric vectors are of particular interest due to the ability to further tune the polymer properties through the incorporation of additional functional units such as targeting ligands or shielding domains. Polyethylenimine (PEI), a highly cationic polymer, is often considered a benchmark for polymer-based …

Developments In Gold(Iii) Scaffolds For Protein Bioconjugation And Enhanced Anticancer Activity, Sailajah Gukathasan

Theses and Dissertations--Chemistry

Site-selective modifications of target proteins using specially designed small molecules is a powerful tool that has been extensively utilized in biomedicine. Small molecules can modify proteins either covalently or non-covalently depending on their structures and intrinsic chemical reactivity. Covalent chemical modification presents a more stable and often irreversible interaction with target proteins; unlike non-covalent binders, which form weak, reversible interactions with protein. Therefore, covalent modifiers represent an effective class of therapeutics due to their stability and irreversibility once bound to target proteins of interest. I hypothesized that tuning biocompatible, high-valent gold(III) complexes toward nucleophile-induced reductive elimination will lead to covalent …

The Design And Synthesis Of New Organic Dithiols For Environmental Applications, Shashika Nadishani Bandara

Theses and Dissertations--Chemistry

A thiol molecule, 2,6-pyridinediamidoethanethiol (PB9), was synthesized based on the pyridine-2,6-dicarboxamide scaffold with appended cysteamine groups. PB9 acts as an effective chelator for Pb(II) due to multiple binding sites (N₃S₂) through irreversible binding precipitating Pb(II). Removal of aqueous Pb(II) from solution was demonstrated by exploring the effects of time, initial PB9:Pb(II) ratios, pH, exposure time, and solution temperature. After 15 min the Pb(II) concentrations were reduced from 50.00 ppm to 0.30 ppm (99.4%) and 0.25 ppm (99.5%) for PB9:Pb ratios of 1:1 and 2:1, respectively. Removal of > 93% Pb(II) was observed over multiple pH values with …

Bolstering Leadership Engagement To Community Coalition Work In Rural Appalachia, Melissa Slone

DSW Capstone Projects

Rural Appalachian communities are overwhelmed with high cancer rates, diabetes, disabilities, and substance use disorder. The challenges in addressing these increase when you factor in workforce shortages and limited funding. The literature documents the effectiveness of coalitions in addressing healthcare challenges within communities. The need is for leaders to reduce their hesitancy to engage in community coalition efforts and increase awareness of the impact community coalitions can have on addressing rural populations' health and well-being needs.

Product One was a systematic review focused on research that examined agency leaders’ perspectives on community coalition involvement. The purpose of conducting the systematic …

Children And High Conflict Divorce: Using Equine-Assisted Psychotherapy As Treatment, Ashley Berry

DSW Capstone Projects

The act of divorce has been commonplace throughout the world during the 20^th century, plateauing around the 1970s at around 50% of all marriages. While most divorces end peacefully, many contain ongoing conflict which can become detrimental to all involved, including the children. While historical treatment methods focus on changing the behavior of parents, there are instances where these methods may be ineffective, especially when the parents are unable or unwilling to change their conflictual behaviors. Equine-Assisted Psychotherapy (EAP), which has been used with individuals who have suffered from trauma, may be an option to use with this population …

Meta-Practice For The Bachelor Level: A Needed Compliment To The Generalist Model, Patrick Callahan

DSW Capstone Projects

Meta-practice in this concept is the web or roadmap, that is, the interconnectedness of the three pillars of the generalist model of social work. The generalist model teaches that micro, mezzo, and macro are interconnected. However, students and new professionals often do not understand precisely what the connection is. This work gathers the available literature, the conceptual idea of the introduction for the bachelor level of education, and an application of introducing it in a course for instruction. There is a need for introducing meta-practice at the bachelor’s level because of the continued globalization of society. By teaching the concept …

Defining Posttraumatic Stress In Law Enforcement: Normalizing Trauma Response, Britta D. Caudill

DSW Capstone Projects

Significant inequalities exist within the expectations of police officers regarding trauma exposure and the lack of mental health preventive efforts and treatment opportunities. The prevalence of stigma thrives within the thought process of this culture paired with the expectation for resiliency, as police culture focuses on the ability to repress emotion instead of utilizing appropriate coping methods. This review takes an interesting look at the history, definition, and diagnostic criteria of post-traumatic stress disorder (PTSD) by focusing on its evolution within the Diagnostic and Statistical Manual (DSM) over time. The primary goal is to establish a relationship between the definition …

Postpartum Depression: Mitigating Treatment Barriers For Women In Appalachia, Tabitha Simpson

DSW Capstone Projects

This capstone focuses on maternal mental needs, specifically postpartum care and postpartum depression. Each article in this capstone centers on postpartum depression, treatment barriers, introducing standard care of practice for women in rural communities, and identifying gaps leading to further research in postpartum education. Countless articles were read and utilized in conducting the systematic literature review to begin this process.

The first article in the capstone, the Systematic literature review, details the signs and symptoms of postpartum depression and how it differs from a major depressive disorder. A thorough review of the literature was conducted, identifying the central concern of …

Good Trouble: Disrupting The Disproportionality Of Black Boys In Special Education Due To Misdiagnosed And Unaddressed Trauma, Montrell Pryor

DSW Capstone Projects

Black boys experience a myriad of trauma as they navigate through society under the gaze of white patriarchy and archetypes. Whether engaging in trauma or witnessing it, it impacts Black boys tremendously, particularly in the educational setting. What typically looks like misbehavior in the classroom can be symptoms of unaddressed and untreated trauma that have been triggered and exacerbated in the school setting. As a result, this phenomenon contributes to the disproportionality of Black boys in special education and adds to the expansion of the school-to-prison pipeline.

This capstone provides a thorough examination of the literature in the form of …

Experiential-Based Feedback During Alcohol Intoxication And Its Effect On Drinkers’ Risk Awareness, Alexandra R. Kelly

Theses and Dissertations--Psychology

The prevalence of alcohol-impaired driving injuries and fatalities has not decreased for over a decade despite strategies to reduce and prevent driving under the influence of alcohol (DUI). In 2019, 10,142 people died in alcohol-impaired driving crashes in the United States, which totaled to 28% of all traffic fatalities for the year. DUI interventions have been found to have moderate effects on reducing DUI recidivism. Prevention research has identified a lack of risk awareness in DUI offenders and this could be a target to motivate behavioral change. However, the training to increase levels of risk awareness needs to occur in …

Effects Of An Online Insomnia Intervention On Sleep And Alcohol Consumption, Justin Verlinden

Theses and Dissertations--Psychology

Alcohol use disorder (AUD) and insomnia are highly comorbid, due in part to the use of alcohol as a sleep aid among individuals with insomnia. Initial studies have shown that cognitive behavioral therapy for insomnia (CBT-I), the first-line treatment for insomnia, is moderately successful at improving both sleep and drinking outcomes in heavy drinkers with insomnia. However, CBT-I is expensive and not widely available. Online CBT-I could be a more accessible alternative. One online CBT-I program, Sleep Healthy Using the Internet (SHUTi), is especially promising due to its high efficacy in treating insomnia and its individualized and interactive platform. Here …

‘I Went To The One Game In Town’: Obstetric And Maternity Unit Closures, Dwindling Birth Choices, And Resilience In Rural Appalachia, Sia Beasley

Theses and Dissertations--Anthropology

Obstetric and maternity health services are being rapidly eliminated in the rural United States, making maternal care more difficult to access and causing negative birth outcomes. Service closures have a magnified impact in Appalachia due to histories of systemic regional and state policy practices which devalue the lives of people living rural areas, local economic marginalization, geographic barriers, and insufficient health infrastructures. This research investigates how women living in rural Appalachia navigate pregnancy and birth amidst constant care closures. The Sunflower Mountain Region is a seven-county area in rural Southern Appalachia. The region has experienced ongoing obstetric closures over the …

Fantasies Of Race And Place: White Nationalist And Alt-Right Undercurrents In Fantasy Roleplaying Games, Mark Hines

Theses and Dissertations--English

Representations of fantasy settings in roleplaying games often draw upon understandings of the medieval and early Renaissance world. This dynamic often extends to racial politics in such worlds. For the contemporary roleplaying game, this often means that game mechanics are built around race, species, or gender. Often, players interpret such mechanics as a means of bioessentializing race or practicing stereotypes rooted in Eurocentric morality and values.

This thesis examines the underlying rhetoric and implicit stakes by which race in fantasy worlds overlaps with the rhetoric and proposed stakes of White Nationalist and Alt-right actors. As fantasy roleplaying games, and especially …

Twenty-First Century Adaptations Of Early Twentieth Century American Protest Literature, Kathryn J. Mcclain

Theses and Dissertations--English

Twenty-First Century Adaptations of Early Twentieth Century American Protest Literature examines the resurgence of didactic political literature in the United States during the 21st century, specifically adaptations of early 20th century American leftist protest works by authors such as Upton Sinclair, Jack London, and Richard Wright. While the most political aspects of these writers’ fiction are often either criticized as too politically overt – such as Sinclair’s The Jungle and Wright’s Native Son – or forgotten in favor of an author’s perceived literary merit – London’s The Iron Heel in comparison to his other works like Call of the Wild …

Single-Cell Analysis Of Aneurysmal Aortic Tissue In Patients With Marfan Syndrome Reveals Dysfunctional Tgf-Β Signaling, Ashley Dawson, Yanming Li, Yang Li, Pingping Ren, Hernan G. Vasquez, Chen Zhang, Kimberly R. Rebello, Waleed Ageedi, Alon R. Azares, Aladdein Burchett Mattar, Mary Burchett Sheppard, Hong S. Lu, Joseph S. Coselli, Lisa A. Cassis, Alan Daugherty, Ying H. Shen, Scott A. Lemaire

Saha Cardiovascular Research Center Faculty Publications

The molecular and cellular processes leading to aortic aneurysm development in Marfan syndrome (MFS) remain poorly understood. In this study, we examined the changes of aortic cell populations and gene expression in MFS by performing single-cell RNA sequencing (scRNA seq) on ascending aortic aneurysm tissues from patients with MFS (n = 3) and age-matched non-aneurysmal control tissues from cardiac donors and recipients (n = 4). The expression of key molecules was confirmed by immunostaining. We detected diverse populations of smooth muscle cells (SMCs), fibroblasts, and endothelial cells (ECs) in the aortic wall. Aortic tissues from MFS showed alterations …