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Novel Mechanism Of Endogenous Pancreatic Cancer Cell Expression Of Immune Checkpoint Programmed Cell-Death 1 Protein (Pd-1) Inducing Epithelial-To-Mesenchymal Transition (Emt) Through The Met Pathway And Promoting Cancer Progression In An Immune-Independent Process, Megan M. Harper

Theses and Dissertations--Clinical and Translational Science

Pancreatic ductal adenocarcinoma (PDAC) remains one of the deadliest cancers with few treatment options, necessitating an urgent need for novel therapeutics. Immuno-oncologic (IO) therapies have revolutionized anti-cancer regimens in the past decade but typically involve reactivation of adaptive immune responses. In particular, immune checkpoint PD-1 is traditionally expressed only on immune cells while PD-L1 (PD-1 ligand) is overexpressed on cancer cells. When tumor-endogenous PD-L1 binds the PD-1 receptor on T-cells, the immune cells undergo anergy resulting in self-tolerance and cancer cell immune evasion. However, contrary to standard dogma, we previously demonstrated tumor-endogenous PD-1 expression in PDAC. Our data indicated that …

Art Education In Medical Education: Benefits And Challenges, Sara K. Brown

Theses and Dissertations--Art and Visual Studies

Humanity is synergistic with art and medicine. Likewise, art education can be impactful throughout medical education. Art as a tool to develop the next generation of healthcare differs from the clinical goals of creative art therapies. Over the prior decade, many medical schools now provide curricular offerings in the arts and humanities. Less is known about the application in postgraduate medical settings. The focus of this thesis is to review the pairing of artist-educators with postgraduate medical training programs.

One such program is ArtsCAFE (Arts Connect Around Food and Enrichment), an intercollegiate project fusing experienced arts educators with medical educators …

A Study Of The Analysis Of Hemp-Derived Oil Products: Development Of A Method For The Analysis Of Phytocannabinoids In Medicinal Products, Erin Johnson

Theses and Dissertations--Pharmacology and Nutritional Sciences

Human interactions with cannabis have a history spanning millennia as a source of fiber, food, and medicine. The plant came across Asia, Europe, and Africa with the movements of people and travelers eventually coming to the Americas with European explorers. In colonizing North America, cannabis fiber was key to the production of canvas and ropes for ships. Although fiber was the driver for cannabis production in the United States, medicinal uses were prevalent and propelled the eventual regulations. The Pure Food and Drug Act of 1906 mandated accurate labeling on drugs such as cannabis followed by the Marihuana Tax Act …

Modeling The Effects Of Adverse Childhood Experiences Using A Mouse Model Of Neglect: Mechanisms Of Adiposity Expansion In Females, Jacqueline Leachman

Theses and Dissertations--Pharmacology and Nutritional Sciences

Adverse childhood experiences (ACEs) are an independent risk factor for chronic disease, including obesity and metabolic syndrome. We have previously shown that a mouse model of early life stress, maternal separation and early weaning (MSEW), exacerbates high-fat diet (HF)-induced obesity only in adult female mice. In agreement, the analysis of several cohorts of participants exposed to ACEs have shown that women are more susceptible to develop obesity than men. Therefore, the aim of this study was to investigate the link between early life stress and obesity in pre-clinical and clinical settings. Male and female MSEW mice fed 1 week a …

Apoe Genotype And Cerebral Glucose Metabolism: A Multi-Omics Approach, Holden C. Williams

Theses and Dissertations--Physiology

Apolipoprotein E (APOE) is encoded by the APOE gene, present in humans as three main isoforms (E2, E3, and E4). E4 carriers face up to a 15-fold increased risk for developing late-onset Alzheimer’s disease (AD), while E2 carriers are protected. Understanding the risk conferred by E4 has been an extensive research focus for nearly three decades, but the exact mechanism has yet to be proven. Many studies have demonstrated attenuated roles of E4 in classical hallmarks of AD, notably amyloid processing and neurofibrillary formation, which normally present later in disease progression. How APOE influences hallmarks that present much earlier are …

Immunoregulatory Receptor Genetics, Expression, And Splicing Studies In Alzheimer’S Disease, Benjamin C. Shaw

Theses and Dissertations--Physiology

Microglia are the resident immune cells of the brain, undertaking many critical tissue maintenance functions such as immune surveillance and phagocytosis. Microglial dysfunction has recently been identified as a multi-stage signature of many neurodegenerative diseases, including late-onset Alzheimer’s Disease (LOAD). Genome-wide association studies (GWAS) have identified single nucleotide polymorphisms (SNPs) in over thirty genes that modulate risk of developing LOAD. In the central nervous system, roughly half of these LOAD-associated genes are primarily expressed in microglia. The proteins encoded by these genes include cell surface receptors that contain either immunomodulatory tyrosine-phosphorylated activating motifs (ITAMs) or inhibitory motifs (ITIMs), including TREM2 …

Farmers’ Market Incentive Program As An Intervention For Improving Behavior Regarding Fruit And Vegetable Consumption In Children And Adolescents, Madison Blades

Theses and Dissertations--Nutrition and Food Systems

Background: Previous research shows that there is low consumption of fruits and vegetables in children and adolescents, specifically those residing in the state of Kentucky which is ranked the lowest of all states in fruit and vegetable (F/V) consumption. The lack of F/V in a diet of someone during their developmental ages can result in negative health outcomes in the future.

Objective: To determine if the use of a farmers’ market incentive program as a nutritional intervention affects the consumption of F/V in children and adolescents.

Methods: 261 children and adolescents, ages 3-14, from 160 families participated in a 10-week …

An Examination Of Organic Options In Tomato Systems And Their Use As Alternatives To Copper-Based Products, Erica Ann Fealko

Theses and Dissertations--Plant Pathology

Organic farming is an ever-increasing segment of tomato production. Currently, limited information is available which directly compares conventional to organic treatment programs for disease control in tomato production. Furthermore, many methods available rely on the use of copper products which may be contribute to high Cu levels in agricultural soils. In this study, the efficacies of current conventional and organic methods were compared. In addition, newer disease-control programs, with and without copper were examined, which potentially could reduce over-reliance on copper products.

Standard organic and conventional spray programs were conducted over a four year period targeting two pathogens, Alternaria tomatophila …

The Effects Of Cannabidiol On Senior Equine Health, Shelley Turner

Theses and Dissertations--Veterinary Science

Cannabis sativa L., has been revealed to produce hundreds of phytocannabinoids, of which cannabidiol (CBD) is one of the most desired. It has been revealed that CBD can potentially treat inflammation and act as an analgesic in humans without psychoactive effects of delta-9-tetrahydrocannabinol (THC). Recently, there has been interest in understanding the potential health benefits of CBD for horses. With an increasing senior horse population (< 15 years old), alternatives to the use of non-steroidal anti-inflammatory drugs (NSAIDs) such as phenylbutazone, firocoxib, and flunixin meglumine, are desired as these common anti-inflammatory treatments have negative side effects. Because senior horses may have chronic low-grade inflammation, termed inflamm-aging, they are a natural model to investigate the efficacy of CBD on inflammatory responses as well as other health parameters. Given this interest, and due to the lack of research conducted on CBD in the horse, the overall objective of this dissertation work was to conduct both basic and applied research investigating the effects of CBD on equine immune function and health. The first objective was to determine the in vitro effects of CBD as an anti-inflammatory. Peripheral blood mononuclear cells (PBMCs) from senior horses were cultured in vitro with increasing concentrations of pure CBD dissolved in dimethyl sulfoxide (DMSO). These cells were …

Effects Of Physiological Stage And Diet On Drosophila Suzukii Olfaction, Aerianna Shindindowi Littler

Theses and Dissertations--Entomology

Massive economic damage by spotted-wing drosophila (SWD), Drosophila suzukii, results from their unique egg laying behavior. Gravid SWD flies employ a complex suite of behaviors to find fruit with the optimum firmness and chemistry. We tested for SWD attraction to various fruits and found overripe raspberries to be most attractive to gravid flies which was unexpected since they oviposit in ripe fruit. We next tested the same choice assay in a T-maze and found that the results were comparable. We also found discrete odor profiles among the ripening stages. When testing for sugars and CO2 concentrations from field-collections, overripe …

Gene Silencing Provides Insights Into Bark Beetle Biology And Creates Potential For Broad Scale Forest Protection, Beth R. Kyre

Theses and Dissertations--Entomology

Dendroctonus bark beetles are among the most economically and ecologically significant forest pests in North America and play a critical role in the overall health of conifer forest ecosystems. Dendroctonus bark beetles influence ecosystem benefits and biodiversity and drive forest succession, and adversely affect timber production, forest management, and recreation. As temperatures surge and climatic fluctuations become more extreme, catastrophic bark beetle outbreaks are increasing in frequency, escalating pressures on highly vulnerable conifer forests already compromised by heat and drought. Eruptive outbreaks of Dendroctonus beetles are largely unhindered by traditional silvicultural management; these practices further disrupt forest ecosystem services, including …

A Functional Genomics Approach To Overwintering Mechanisms In Insects, Emily Allison Wheeler Nadeau

Theses and Dissertations--Entomology

Insects living in temperate and polar environments have developed numerous adaptations to increase survival at low temperatures. A majority of insects are freeze-intolerant and die from internal ice formation, but some are freeze-tolerant and can survive ice formation in extracellular spaces. Both categories of insects prepare for winter with a combination of seasonal and rapid acclimation responses, which differ both in time course and in underlying mechanisms. In this dissertation, I examine adaptations for winter survival in several insect species with a specific focus on molecular mechanisms. To better understand the underpinning mechanisms of these adaptations, I leverage functional genomics …

Attentional Bias, Inhibitory Control, And Food Reinforcement As Mechanisms Promoting Eating Behavior After Exercise, Kylie F. L. Mcfee

Theses and Dissertations--Nutrition and Food Systems

Background: Excess weight and obesity are serious health conditions characterized by modifiable and nonmodifiable risk factors. Exercise is a common method for achieving weight loss; however, results are not always achieved due to post-exercise compensatory behaviors. Increased energy intake (EI) is thought to be the main compensatory behavior hindering weight loss among overweight and obese adults. It is important to determine and understand the mechanisms behind energy compensation following exercise, as this will allow for the discovery of future interventions that may provide individuals with ways to improve exercise as a weight loss treatment.

Objective: To determine if a single …

The Influence Of Sociopolitical Factors On Adolescents’ And Youths’ Development, Banafsheh Aghayeeabianeh

Theses and Dissertations--Family Sciences

Youths and adolescents are one of the major perpetrators of antisocial and deviant behaviors, which have deleterious consequences for both the perpetrators and society. Although there is extensive literature on youth and adolescent antisocial behavior, some correlates of youth antisociality are not known yet. As such, the present study is devoted to understanding the micro- and macro-level predictors of youth and adolescents’ antisociality in three contexts. Three empirical studies applying bioecological systems theory and analyzing data from the International Dating Violence database using Mixed Effects Models were conducted to investigate the ecology of the development of antisocial behaviors among youth. …

Pilot Study: The Role Of Online Grocery Shopping In Meal Planning & Prepartion: Utilizing Text Message Based Behavioral Nudges, Brittany Nikole Cox

Theses and Dissertations--Nutrition and Food Systems

Purpose: To examine if an online grocery shopping intervention improves meal planning and preparation and subsequent dietary intake. The primary hypothesis was that online shopping can improve meal planning and meal preparation habits while also improving dietary intake.

Method: Participants were randomized to either online only (O), online plus nutritional nudges (O+I), or standard brick and mortar (BM). Those in the O+I arm received behavioral nudges three (3) times per week and were part of a Facebook group assisting them with meal planning, nutrition education, and reminders to shop online. The O arm only received reminders to shop online, and …

Associations Of Sexual Satisfaction, Relationship Quality, And Cognitive Functioning Within Intimate Relationships In Older Adulthood, Allison G. Smith

Theses and Dissertations--Family Sciences

The roles of sexual satisfaction, relationship quality, and cognitive functioning within older adults (N = 538) intimate relationships are assessed at two timepoints over an 11-year timeframe. Longitudinal data from 63 older adults are assessed through a path modeling approach to examine how sexual satisfaction and relationship quality change throughout older adulthood. Cross-sectional data for 538 older adults are utilized to examine the association between cognitive health and sexual satisfaction/relationship quality. Qualitative data from 125 older adults are utilized to understand how older adults describe their intimate relationships. Results from path models demonstrate that one’s degree of sexual satisfaction …

Trauma-Sensitive Leadership In Multidisciplinary Settings, Jennifer Griswold Withrow

DSW Capstone Projects

As the social work profession continues to expand into more diversified areas of practice, such as politics, environmental justice, governmental work, and international crisis work, it is critical to ensure that social workers have the tools necessary to be successful leaders in these extended areas. Utilizing Trauma-Sensitive Leadership is an important concept to incorporate into multidisciplinary teams to ensure employees and communities feel supported and understood. By implementing these concepts which are ingrained into the social work profession into expanded areas of practice, social workers can feel empowered to lead multidisciplinary teams and provide support and understanding to the staff …

Utilizing A Video-Based Intervention To Improve Medicine Adherence And Retention Of Hiv Care In A Hiv Clinic., Robert Currie

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Memphis, Tennessee has higher rates of black men who have sex with men living with human immunodeficiency (BMSMLWH) compared to state and national levels. ART has been shown to be effective in keeping people living with HIV virally suppressed and unable to transmit HIV through sex. However, BMSMLWH are disproportionately affected by HIV than other races in Memphis, Tennesee. The proposed program relies on evidence-based data that demonstrates the effectiveness of video-based intervention in improving medication adherence and HIV retention of care. Taking Care of Me is a video-based intervention that promotes HIV treatment initiation, ART adherence, and retention in …

What Impact Does The Nursing Shortages Have On The Financial Health Of The Kentucky Healthcare System?, Michelle Cooper

MPA/MPP/MPFM Capstone Projects

This study investigates how the nursing shortage affects the financial health of healthcare systems. Further, it examines the economic impact that healthcare systems face due to the nursing shortage and what they are doing to cope during this time to meet their nursing staff needs. The study focuses on hospitals in Kentucky and compares Kentucky to other states in the United States. This research aims to determine how the nursing shortage financially impacts Kentucky hospitals. The main question that this research paper will address is what impact do the nursing shortages have on the financial health of the healthcare system? …

Evaluating Pharmacy Student Opinions Of The Kentucky Professionals Recovery Network, Abby Kosharek

MPA/MPP/MPFM Capstone Projects

Healthcare professionals have similar rates of substance use disorders as the general population. Healthcare professionals are in a unique position because their substance use disorder affects them and may affect their patients. Substance use disorders among healthcare professionals can result in lawsuits and loss of licensure. Kentucky Professionals Recovery Network (KYPRN) provides healthcare professionals with substance use disorders services and programs to help them recover from their disorder. Unfortunately, the University of Kentucky College of Pharmacy no longer provides education on the Kentucky Professionals Recovery Network in its formal curriculum. Instead, students are expected to gain this knowledge via experiential …

Economic Development Plans: Creating Remote Worker Incentive Programs, Sadie Barton

MPA/MPP/MPFM Capstone Projects

The decrease in population growth is impacting states and localities across the United States and is anticipated to continue in the future. To address this issue, some areas have created remote worker incentive programs to attract new residents as part of their economic development plans. Although few programs existed before 2020, the Covid-19 pandemic created an opportunity for employees to work remotely more than any other time in history. This resulted in a significant increase in remote worker incentive programs being created. These programs are designed to attract new residents to their area which in turn will have economic impacts …

Examining Ky Hb8 For 2022: What Can We Learn From Other States’ Experiences?, Patrick Welsh

MPA/MPP/MPFM Capstone Projects

Currently, Kentucky levies an individual income tax on all income earned by Kentucky residents at a rate of 5%. A bill approved in 2022 by the Kentucky legislature, KY HB8 for 2022 will phase down the individual income tax rate from 5% to 0% and expand the sales tax to 39 additional classes of services. Incremental decreases in the individual income tax rate depend on meeting General Fund receipts thresholds established in the bill after an initial reduction of one percentage point. Kentucky will join the ranks of 9 other states that do not impose an individual income tax including …

Irs Form 990 And Opportunity For Improvement, Sarah Bocklen

MPA/MPP/MPFM Capstone Projects

In the contemporary world, IRS Form 990 returns are increasingly being used to source data about the nonprofit sector, which have critical in enhancing the community's social welfare. However, there has been a considerable debate concerning the adequacy, reliability, and quality of the data contained in the document. This study incorporated a total of 150 nonprofit entities from the US to investigate the adequacy, reliability, and quality of Form 990 returns. I used both quantitative and qualitative research methods to evaluate the consistency in the selected data. Selected entries were used for analysis and compared with those of audited financial …

Effects Of Emergency Medical Service Funding Policies In Kentucky, Amber Moreland

MPA/MPP/MPFM Capstone Projects

Emergency medical services is a complicated component in local Kentucky governments. Emergency Medical Services (EMS) operated and managed by local government have various funding mechanisms and each have different methods of reporting. This paper reviews the various ways EMS is funded in Kentucky along with the impact these different funding strategies have on quality and delivery of emergency medical services.

Investigating Mechanisms Of Injury And Intervention In A Novel In Vitro Model Of Traumatic Brain Injury In Organotypic Hippocampal Slice Cultures, Julia Elaine Jagielo-Miller

Theses and Dissertations--Psychology

Traumatic brain injuries (TBIs) impact millions of individuals each year and can pose long term consequences. Despite numerous attempts, no medication has been approved by the FDA to treat TBIs. The causes of these failed trials are multifaceted, but in part can be attributed to the complex nature of TBIs, as well as a lack of sufficient pre-clinical data. In vitro models of TBI are an important tool to help understand the cellular changes seen following the injury, in a highly controlled environment. For the following experiments, a novel model of TBI was used to injure organotypic hippocampal slice cultures, …

Using Stream Restoration To Mitigate Stormwater Runoff In An Urban Watershed: A Case Study, Jonathan M. Brantley

Theses and Dissertations--Biosystems and Agricultural Engineering

The goal of this case study was to evaluate the effectiveness a 275 m regenerative stream restoration design to mitigate stormwater runoff in a highly urbanized watershed. The restoration resulted in a wide, wetland-like floodplain, comprised of a rock base that was overtopped with a filtration media (approximately 30% woodchips and 70% topsoil). The creation of the floodplain-wetland complex in tandem with filtration media lead to increased storage capacity and an increase in hyporheic exchange within the system. Significant reductions were found for the storm hydrograph parameters volume, peak discharge, and time to peak. Reductions in baseflows were also noted …

Supporting Stylized Language Models Using Multi-Modality Features, Chengxi Li

Theses and Dissertations--Computer Science

As AI and machine learning systems become more common in our everyday lives, there is an increased desire to construct systems that are able to seamlessly interact and communicate with humans. This typically means creating systems that are able to communicate with humans via natural language. Given the variance of natural language, this can be a very challenging task. In this thesis, I explored the topic of humanlike language generation in the context of stylized language generation. Stylized language generation involves producing some text that exhibits a specific, desired style. In this dissertation, I specifically explored the use of multi-modality …

Smart Decision-Making Via Edge Intelligence For Smart Cities, Nathaniel Hudson

Theses and Dissertations--Computer Science

Smart cities are an ambitious vision for future urban environments. The ultimate aim of smart cities is to use modern technology to optimize city resources and operations while improving overall quality-of-life of its citizens. Realizing this ambitious vision will require embracing advancements in information communication technology, data analysis, and other technologies. Because smart cities naturally produce vast amounts of data, recent artificial intelligence (AI) techniques are of interest due to their ability to transform raw data into insightful knowledge to inform decisions (e.g., using live road traffic data to control traffic lights based on current traffic conditions). However, training and …

Image Geo-Localization With Cross-Attention, Connor Greenwell

Theses and Dissertations--Computer Science

The problem of estimating the location from which un-geotagged photographs were captured has been well studied by the computer vision community in recent years. The central proposal of this thesis is to define a common framework within which existing approaches can be constructed and evaluated, and to introduce a new method under this framework which uses cross-attention between the query image and a database of satellite imagery with known geotags. Our experiments fit within three broad categories: 1) evaluating the ability of image localization approaches to generalize to unseen regions; 2) examining performance changes under various reference database resolutions, scales, …

We Can Still Feed Ourselves: Food Sovereignty, Aid, Sickness, And Health In Eastern Kentucky, Annie Koempel

Theses and Dissertations--Anthropology

Over forty percent of eastern Kentucky residents are classified as obese. From a biomedical perspective, obesity is linked to a range of chronic diseases, including heart disease, diabetes, and high blood pressure and is caused by particular lifestyle behaviors that lead to an increase in calorie consumption and decrease in calorie expenditure. However, these links – individual behavior leads to obesity which leads to chronic disease - do not take into account a wide range of personal, social, environmental, political, and economic conditions. In addition to the assumptions of what it means to become and be obese, Kentucky is regularly …