Digital Commons Network^™

Microglial Apoe Across Alzheimer’S Disease-Relevant Pathologies, Georgia Nolt

Theses and Dissertations--Physiology

APOE exists as three common alleles (APOE2, APOE3, APOE4) that encode their corresponding protein isoforms (apoE2, apoE3, apoE4). Despite differing by only one or two single-amino acid substitutions, each APOE allele confers a distinct AD risk profile with APOE4 increasing risk by 28% and APOE2 decreasing risk by 14% compared to the most commonly expressed APOE3 allele. While traditionally considered “neutral,” APOE3 still accounts for ~36% of AD cases while APOE4 contributes ~57%, indicating that the majority of AD cases would not occur in the absence of the APOE3 and APOE4 alleles. Microglia, the resident phagocytic immune cells …

The Relationship Between Participants In The Certificate Of Educational Technology And Information Literacy (Coetail) Program And Professional Capital, Benjamin J. Sheridan

Theses and Dissertations--Educational Leadership Studies

This study aimed to explore how educators involved in the Certificate of Educational Technology and Information Literacy (COETAIL) program perceived the development of their professional capital within an online, cohort-based professional learning community. Based on Hargreaves and Fullan’s (2012) concept of professional capital, which includes human, social, and decisional capital, and guided by social learning theories, the study examined participants’ perceptions of professional capital development, the program features related to that development, and how participation influenced their professional paths. The research focused on international school settings, where educators often rely on networked professional learning because of geographic mobility and limited …

Hallucinogen Use Among Parents Of Children In The United States: Associations With Mental Health And Substance Use, Maggie K. Richardson

Theses and Dissertations--Educational, School, and Counseling Psychology

Hallucinogen use has reemerged in the United States amid expanding clinical research and shifting social and policy contexts. However, population-based evidence remains limited regarding hallucinogen use among adults living with children and its relationship with mental health impairment severity in caregiving environments. Therefore, this study’s objective was to examine associations between past-year hallucinogen use and past-year mental health impairment among U.S. adults, and to test whether associations differ by parental status and number of children in the household. A secondary aim examined correlates of co-occurring substance use among those reporting hallucinogen use.

Data were drawn from the 2023 National Survey …

Bureaucratic Responsiveness In Times Of Crisis, Anna Gunderson, Maggie Macdonald

Political Science Faculty Publications

In 2020, American cities experienced mass protest after fatal police violence against Black Americans. Did police address these protests differently after the murder of George Floyd or the protests in their communities? Did that response vary depending on the racial or ideological makeup of their constituents? We link policing protests and Facebook posts from about 4,000 American police departments to investigate differences in agency responses to protest. Departments posted more about protest after Floyd's death, especially in those communities that also had a policing protest. Republican cities were less likely to post about protests, but racial demographics are not associated …

Insights From And Future Directions For A Nationwide Science And Engineering Education Collaboration In Stratospheric Ballooning, Beth A. Covitt, Angela C. Des Jardins, Erick P. Agrimson, Jason T. Ash, Sean C.C. Bailey, Katelyn A. Barber, Mike J. Bastidas, Edgar A. Bering, Matthew T. Bernards, Anthony Choi, Nicholas B. Conklin, Jacqueline K.Q. Do, Montana Etten-Bohm, William F. Finney, James A. Flaten, Jeffrey M. Freedman, Rachel H. Humphrey, Eric P. Kelsey, Hunmin Kim, Christopher L. Lee, Wookwon Lee, Jared W. Marquis, Justin R. Minder, Jani M. Pallis, David J. Pawlowski, Chong Qiu, Joanna H. Rivers, Jason A. Ruszkowski, Jillian B. Schmidt, Thomas G. Sharp, Paul E. Slaboch, Suzanne W. Smith

Mechanical Engineering Faculty Publications

The Nationwide Eclipse Ballooning Project (NEBP) engaged 53 teams of students from across the United States to fly experiments on high-altitude balloons for the 2023 annular and 2024 total solar eclipses. By many measures, NEBP was a successful project. However, the teams did encounter challenges. To better understand what could be improved for similar future projects, this study engaged project partners in examining strengths, challenges, and recommendations across four topics: project structure, education and research approach, broadening participation, and funding. Analysis of the topics was completed through written comments followed by remote focus group discussions. The project director and evaluator …

The Impact Of A Kentucky Nutrition Education Program Social Marketing Campaign On Resident Health, Trinity Elizabeth Foster

Theses and Dissertations--Agricultural Economics

Poor diet quality and physical inactivity are common health issues in the United States. To remedy these issues among low-income households, the United States Department of Agriculture created the Supplemental Nutrition Assistance Program Education Program (SNAP-Ed), which promoted healthy eating and physically active lifestyles through multiple avenues. The social marketing approach of SNAP-Ed uses marketing strategies to relay information to the public. In Kentucky, a statewide billboard campaign was launched in 2018 encouraging residents to focus on nutrition and physical activity. This study investigates the impact of that campaign on health outcomes in counties throughout the state. Using billboard location …

How Does Residing In A Battleground State Explain Republican Elites Election-Denial Rhetoric On Social Media?, Caedmon P. Brewer

Theses and Dissertations--Political Science

After the 2020 election the U.S. democratic process was challenged by Republican challengers many of which were office holders including the president. Many of these criticisms were shared and perpetuated in online spaces What encourages elites to post on social media about election denialism? This paper attempts to partially answer the question by performing an analysis on Facebook posts made by 1,154 congressional candidates between 2023 and 2024 using a dictionary search method to flag posts for election denialism. I propose a theory that suggests the combination of political, financial, and media attention given to battleground states encourages candidates in …

Facebook As A Strategy To Deliver A Peer Navigation Intervention For Recently Incarcerated Women With Opioid Use Disorder: A Mixed Methods Descriptive Profile, Martha Tillson, Jaxin Annett, Marguerite A. Webster, Amanda Fallin-Bennett, Mandi Webster, Amber Clemons, Cynthia Robinson, Kevin Crabtree, Casey Baker-Romans, Michele Staton

Center on Drug and Alcohol Research Faculty Publications

Recovery support and treatment linkages are critical during transitions from incarceration to community but can be challenging to deliver to hard-to-reach populations. This paper will examine Facebook contacts between peer navigators (PNs) and women with opioid use disorder (OUD) following jail release to explore women’s use of PN services, including purpose and content of Facebook messages. As part of a larger clinical trial under the Justice Community Opioid Innovation Network (JCOIN), PNs provided OUD treatment navigation and recovery support for 12 weeks following women’s release from jail, using remote options such as Facebook. Facebook Messenger transcripts between PNs and participants …

Contemporary Commercial Music: A Scoping Review With Implications For Music Therapy Practice, John A. Lawwill

Theses and Dissertations--Music

Contemporary Commercial Music (CCM) is an increasingly popular group of non-classical musical styles. Music therapists need to know how to sing a wide variety of CCM genres authentically, yet little information exists on effectively teaching vocal CCM techniques to music therapists. The purpose of this study was to conduct a scoping review on CCM singing instruction and to synthesize implications for music therapists. I used EBSCOhost and ProQuest to search for reports in English published after 2002 containing the terms “Contemporary Commercial Music” or “CCM,” and variations of the term “sing.” I identified 26 reports that met inclusion criteria, including …

Structuring The Musical Year: Dance, Topic, And Semiosis In Eighteenth-Century Calendar Music, Braden Shaw

Theses and Dissertations--Music

In this thesis, I apply a multifaceted analytical approach to examine a niche category of music called calendar music. In chapter one, I discuss Gregor Joseph Werner’s composition, the New and Very Curious Musical Calendar, and specifically address the minuets found within this work. Werner composes irregular measure groupings in his binary minuets and did so to represent the number of hours in day and night. This irregularity of measure groupings might superficially suggest that Werner’s pieces are stylized minuets only, unintended to accompany physical dancing and far removed from any actual choreographic influence of embodied performance. Werner, …

Belting In Musical Theatre: Vocal Pathologies, Injuries, And Pedagogical Implications, Kaylee Piatt

Theses and Dissertations--Music

Belting has become one of the most defining and contested vocal styles in contemporary musical theatre. Valued for its dramatic immediacy, speech-like intensity, and ability to cut through amplified orchestration, belting is frequently associated with heightened injury risk and pedagogical controversy. This thesis examines the relationship between belting, vocal pathology, and pedagogy through an interdisciplinary lens that integrates voice science, clinical literature, and professional performance practice. Rather than framing belting as an inherently harmful or stylistically aberrant technique, this study conceptualizes belting as a functional vocal coordination shaped by physiological mechanisms, cumulative vocal load, and occupational demands.

Drawing on existing …

Seriosa And The Song Recital Tradition: Reframing The Antologi Musik Klasik Indonesia: Seri I—Vokal Dan Piano (Seriosa), Pharel Silaban

Theses and Dissertations--Music

This project traces the changing life of seriosa, later known as Indonesian art song, from its colonial emergence in the 1930s–1950s to its post-independence expansion and institutional contraction in the 1960s–1980s, and contemporary reframing since the early 2000s. It argues that the genre functioned as a vocal infrastructure that coordinated training, repertoire production, radio broadcast circulation, and public audibility. The study further shows that when the influence of Radio Republik Indonesia weakened, the fragility of this infrastructure became visible. Efforts to sustain the genre by figures such as Rose Pandanwangi, Pranawengrum Katamsi, Catharina Wiriadinata Leimena, and Aning Katamsi appeared …

Implementations Of Improvisation And Existing Materials In Electroacoustic Composition, Jeffrey Todd-Voyten

Theses and Dissertations--Music

I have developed my compositional process through the use of sound synthesis, existing materials, and improvisation. When combining these techniques and resources, human gestures can emerge from inhuman materials in a dialogue between machines and humans. This thesis is an overview of my initial inquiries into the continuum of human agency and machine intervention, considering aspects of patch-programming, formal structure, and performance practice. In addition to the aforementioned compositional methods, this thesis also considers musico-dramatic meaning and personal musical influences outside the scope of electroacoustic music.

Cantate Fratres: Forming And Sustaining Traditional Catholic Music, Liturgy And Culture In The Institute Of Christ The King Sovereign Priest, Reyers P. Brusoe

Theses and Dissertations--Music

The Institute of Christ the King Sovereign Priest is an understudied member of the Traditional Catholic and Latin Mass Communities whose unique approach to Catholic liturgy, culture, and formation offers a sustainable model for the Catholic Church's longevity and renewal. This study is the first of its kind, combining the theoretical positions of affect theory, ecomusicology/musical sustainability, and the musicology of religion to explore and explain the success of this growing classical community. This dissertation examines Church documents, theological literature, and multidisciplinary academic sources as a foundation for the study.             In studying the Institute of Christ the King Sovereign Priest, …

From County To Commonwealth: A Study Of Kentucky County Election Office Websites And Collaborative Solutions For Strengthened Voter Information Accessibility, Marissa A. Robinson, Marina E. Smart

MPA/MPP/MPFM Capstone Projects

In 2022, the League of Women Voters discovered inconsistencies across Kentucky county clerk websites in how they offer voter information and in website accessibility and clarity. County clerk websites serve as an important source of accessible voting procedural information, trusted by constituents. Through an audit of county clerk websites and interviews with county clerks, this project identifies how county election office websites can better provide equitable, accurate, comprehensive, and consistent voting information. Recommendations based on the findings include linking the Kentucky State Board of Elections Voter Information Portal universally, adding website training, and state-established website guidelines.

The Political Economy Of Pfas Treatment And Water Regionalization In Harrison County, Kentucky, Jackson S. Patru, Rajan Sampat

MPA/MPP/MPFM Capstone Projects

The discovery of per- and polyfluoroalkyl substances (PFAS) in the water supply of Harrison County, Kentucky has prompted an urgent reevaluation of local treatment infrastructure. This situation has exposed a complex political economy between the City of Cynthiana, which owns and operates the municipal water plant, and the Harrison County Water Association (HCWA), which represents the majority of the ratepayers supplied by the plant. Using a qualitative research design grounded in inductive thematic analysis, semi-structured interviews were conducted with leadership from both Cynthiana and HCWA, as well as operators at the treatment plant, to evaluate two proposed solutions: a $14 …

Kentucky Space Futures: Defining The Driving And Limiting Factors Of Growth In The State Aerospace Industry, Ashley M. Grospitch, Isabella A. Sibold

MPA/MPP/MPFM Capstone Projects

Kentucky’s aerospace industry has quietly become one of the state’s most significant economic drivers, generating almost $19 billion in exports in 2024 and ranking third nationally in aerospace exports in 2023 (Mattingly, 2025; Babbage & Hartsough, 2024). Despite this, Kentucky remains largely invisible as an aerospace state, both to Kentuckians and national audiences. This work was conducted on behalf of our client, Kentucky Space Futures, to identify the factors shaping the growth of Kentucky’s aerospace industry, including its driving and limiting factors, and provide actionable guidance for policymakers and industry leaders.

Using a qualitative research design, this study triangulated findings …

Protecting The Vulnerable: Elderly Population Change In Kentucky & Nursing Home Floodplain Proximity, David M. Stevens, Austin Matthew Mullen, Nicklaus P. Hammond

MPA/MPP/MPFM Capstone Projects

This study explores Kentucky’s changing vulnerability to flooding. Relying on America Community Survey (ACS) survey data estimates for 2019 and 2024, we illustrate how Kentucky’s elderly population has changed over this five-year period. Using nursing home data and floodplain spatial data, provided by the Centers for Medicare & Medicaid Services and FEMA respectively, we identify all 269 facilities and their proximity to floodplains across Kentucky. To narrow the scope of our study, we highlight counties that (a) have seen the largest percentage increase in elderly residents and (b) have a large total elderly population. Using a quartile ranking system, we …

Functionalization Of Microfiltration Media Towards Catalytic Hydrogenation Of Selected Halo-Organics From Water, Subrajit Bosu, Samuel S. Thompson, Doo Young Kim, Noah D. Meeks, Dibakar Bhattacharyya

Chemical and Materials Engineering Faculty Publications

Contaminated water detoxification remains difficult due to the presence of persistent halo-organic contaminants, such as perfluorooctanoic acid (PFOA) and chlorophenols, which are chemically stable and resist conventional purification methods. Functionalized membrane-based separation and decontamination have garnered immense attention in recent years. Commercially available microfiltration membrane (PVDF) and polymeric non-woven fiber filters (glass and composite) are functionalized with poly(methacrylic acid) (PMAA) that shows outstanding pH-responsive performance and tunable water permeability under ambient conditions perfect for environmental applications. Polymer loading based on weight gain measurements on PMAA–microglass composite fibers (137%) and microglass fibers (116%) confirmed their extent of functionalization, which was significantly …

C/Ebpα Is Essential For Gonadal But Not Inguinal White Adipose Tissue Formation In Mice, Krista Y. Hu, Yu-Lin Ma, Esme A. Dodge, Olivia A. B. Maguire, Caio V. Matias, Ryan K. Barney, Hector S. Himede, Juliana Gomez Pardo, Miriam Cepeda, Scott M. Gordon, Robert C. Bauer

Physiology Faculty Publications

Objective: The distribution of excess white adipose tissue (WAT) in obesity correlates with risk for comorbidities. Thus, understanding depot-specific WAT developmental mechanisms is translationally relevant. SNPs near the gene CEBPA associate with waist to hip ratio, and while C/EBPα is a recognized regulator of adipogenesis, there is no previously known role for C/EBPα in regulating adipose distribution.

Methods: We crossed Cebpa floxed mice to the AdipoQ-Cre transgenic mouse strain, generating mice with adipocyte-specific knockout of Cebpa (Cebpa_ASKO). Mice were phenotyped on a chow diet and after prolonged high-fat diet (HFD) feeding.

Results: Cebpa_ASKO mice almost entirely lack gonadal …

Autosiq, Liming Zhou, Talukder Z. Jubery, Baskar Ganapathysubramanian, Thomas Lübberstedt, Siddique I. Aboobucker

Plant and Soil Sciences Faculty Publications

Doubled haploid (DH) technology can fast-track crop breeding.Haploid induction yields haploids with only one set of genomes, which are usually sterile.Haploid fertility (HF) is the ability of haploid plants to set seed, and it is a critical bottleneck in DH pipelines.Genetic mechanisms to restore HF hold immense potential in DH crop breeding, yet its phenotyping remains manual, destructive, and inconsistent.While recent advances in imaging and machine learning have improved throughput for general plant traits, no curated image dataset exists for Arabidopsis thaliana that explicitly represents HF.Here, we present AutoSiQ, a dataset and baseline deep learning pipeline for automated HF quantification.AutoSiQ …

Carbon Nanodot Precursors Enable Ultrahigh-Loading Copper Single-Atom Catalysts For Oxygen Reduction Reaction, Prakhar Sharma, Ayanthi Thisera, Matthew G. Boebinger, Jenna K. Rector, Jason M. Unrine, Beth S. Guiton, Doo Young Kim

Plant and Soil Sciences Faculty Publications

Single-atom catalysts (SACs) have attracted significant attention in electrocatalysis due to their high metal utilization and tunable electronic structures; however, their practical implementation is often limited by poor atomic dispersion, low metal loadings (typically < 1 wt%), and insufficient anchoring of single metal atoms on catalyst supports. Herein, we report a simple and versatile strategy to synthesize copper single-atom catalysts (Cu-SACs) with ultrahigh metal loading using citric-acid-derived carbon nanodots as metal-capturing precursors. Through hydrothermal treatment followed by pyrolysis in the presence of urea, atomically dispersed copper atoms coordinated with nitrogen are incorporated into a carbon framework, achieving a Cu loading of up to 20 wt% while maintaining atomic dispersion. Notably, a significant fraction of the copper single atoms exists as Cu⁺–N₂ coordination, which provides under-coordinated and catalytically active sites. The resulting Cu-SAC exhibits promising oxygen reduction reaction (ORR) activity in alkaline media, delivering a limiting current density comparable to that of commercial 20 wt% Pt/C with a predominant four-electron reduction pathway. Despite this high intrinsic activity, durability tests reveal gradual performance degradation under prolonged electrochemical operation, which is attributed to demetallation and aggregation of …

Disrupted Circadian Rhythms Affect Hallmark Pathologies In Alzheimer’S Disease-Related Mouse Models, Valeria Buzinova

Theses and Dissertations--Molecular and Cellular Biochemistry

Alzheimer’s Disease (AD) is a complex neurodegenerative disease with two hallmark pathologies: extracellular amyloid-b (Ab) and intracellular neurofibrillary tangles (NFTs). Ab is proteolytically processed from amyloid precursor protein (APP) by b-secretase and g-secretase as a monomeric peptide prone to aggregation. Eventually, the aggregate-prone monomers will form dense plaques that are difficult to break down and remove. These plaques begin to deposit into the cortex decades prior to the formation of NFTs and the onset of cognitive decline. NFTs are comprised of hyper-phosphorylated tau. Tau is a protein that serves to promote and stabilize the formation of microtubules. The formation of …

Characterizing The Human Metapneumovirus Matrix Protein And Its Role In Infection, Chase J. Heim

Theses and Dissertations--Molecular and Cellular Biochemistry

Human metapneumovirus (HMPV) causes severe respiratory tract infections in all cohorts, but especially in vulnerable groups such as children, older adults, and the immunocompromised. The matrix (M) protein of HMPV, like the M proteins of other members of the Mononegavirales order, is involved in virus assembly and budding. However, other functions of the HMPV M protein have yet to be elucidated. To investigate these various functions of the M protein, we used a peptide-conjugated phosphorodiamidate morpholino oligomer (PPMO) antisense agent designed to block translation of the M gene to reduce M expression during infection. Treatment with varying concentrations of an …

Health Communication Campaign Performance During The Healing Communities Study, Nicky Lewis, Jennifer Reynolds, Diane Krause, Philip M. Reeves, Jamie Luster, Michael D. Stein, Sharon L. Walsh, Amy Farmer, Michelle R. Lofwall, Monica F. Roberts, Hilary L. Surratt, Brooke N. Crockett, Kara Stephens, Kelli Bursey, Kristin Mattson, Michael D. Slater

Psychiatry Faculty Publications

Background: Research on the effectiveness of digital health campaign strategies is lacking. Understanding performance outcomes is essential for the successful implementation of campaigns. Two studies examined platforms, tactics, and content of digital health campaigns using paid media performance data. Objective: This analysis compared 2 digital advertising methods (social media and banner or display) using click-through rate (CTR) and cost-per-click (CPC) as performance measures. Performance differences by state, community type, message approach, format, and image type were assessed. CTR and CPC served as measures in determining performance differences between social media and banner or display. Methods: This cross-sectional secondary analysis examined …

The Role Of Small Extracellular Vesicles In Modulating Radiation Resistance In H3k27m-Pediatric Diffuse Midline Glioma, Viral Oza

Theses and Dissertations--Molecular and Cellular Biochemistry

Pediatric diffuse midline gliomas with H3K27M alteration (H3K27M-pDMG) are the leading cause of pediatric brain tumor-associated deaths. All H3K27M-pDMG are initially treated with fractionated radiotherapy, the standard of care, and most children succumb to their disease within two years of diagnosis. There are no universally effective chemotherapies and full resection is impossible due to the diffuse nature of the tumor and sensitive location. There has not been a significant clinical advancement in more than 40 years.

Tumors become completely resistant to radiation within the first six months of treatment. The mechanism of radiation resistance is unknown but is thought to …

Rna-Micelles As Self-Assembling Structures For Efficient Co-Delivery Of Synergistic Sirna And Nucleoside Analogues To Treat Crc Lung Metastasis, Kai Jin, Piotr G. Rychahou, Daniel W. Binzel, B. Mark Evers, Peixuan Guo

Markey Cancer Center Faculty Publications

SiRNA has been widely studied in cancer gene silencing over the last 25 years. However, few siRNA-based therapeutics have been approved by the FDA. An RNA-micelle platform provides a powerful therapeutic tool through simple one-step, high-yield production that is capable of efficient colorectal cancer lung metastasis treatment, a current lethal condition with a short survival rate post-diagnosis. Here, it is reported that the use of RNA-micelles co-carrying siRNA and nucleoside analogues to completely inhibit lung metastasis of colorectal cancer (CRC). The major advantage of the RNA-micelle is the successful co-delivery of siRNA and chemotherapeutic agent in a single delivery vehicle …

Numerical Analysis And Simulation Of Enhanced Performance In Nanowire Cds/Cdte Solar Cells: A Pathway To Greater Than 25% Efficient Cdte Solar Cell, Riasad Badhan

Theses and Dissertations--Electrical and Computer Engineering

This Thesis finds a pathway to a significantly high-efficient CdTe based solar cell by demonstrating and harvesting the advantages of a nano-structure configuration in CdTe based solar cells. Nanowire CdS window layer and the “control”, planar CdS window layer films were fabricated in the laboratory and compared for their optical transmission and other characteristics affecting the performance of the CdS-CdTe solar cell. Numerical simulations were performed for a comparative evaluation of the embedded nanowire CdS-CdTe solar cell device and the traditional planar CdS-CdTe solar cell device. Experimentally measured spectral transmission of nanowire CdS film was used in the simulation environment. …

Making Sense Of Membership: Organizational Socialization Of African Immigrant Employees In U.S. Workplaces, Ikeola J. Bodunde

Theses and Dissertations--Communication

This dissertation examines how African immigrant employees make sense of organizational socialization in U.S. workplaces. As global mobility reshapes the labor force, nearly one in five U.S. workers is foreign-born, making organizational socialization an increasingly complex communicative process. Classic socialization models assume linear, uniform adjustment, yet international employees often face ambiguity, cultural mismatch, and racialized expectations that complicate early workplace experiences. Guided by sensemaking theory, this study conceptualizes socialization as an ongoing, nonlinear process through which newcomers notice cues, construct interpretations, act on provisional understandings, and revise meanings as contexts shift. Using a qualitative, interpretive design, I conducted semi-structured interviews …

Implementing A Competency-Based Cross-Training Program To Enhance Patient Flow, Jill E. Shanklin

DNP Projects

Objective: To evaluate the effect of a competency-based cross-training program on perinatal nurses’ preparedness to care for medical-surgical (med-surg) overflow patients and to support patient flow.

Design: Quality improvement project using a quasi-experimental pre–post design.

Setting: A 44-bed perinatal unit within a university-affiliated medical center in the United States.

Participants: Registered nurses employed in the perinatal unit for ≥6 months; 24 completed the preintervention survey and 28 completed the postintervention survey.

Interventions: A competency-based cross-training program consisting of e-learning modules, simulation-based competency validation, and structured policy and procedure review.

Main Outcome Measures: Self-reported comfort, …