Digital Commons Network^™

Beliefs And Knowledge Related To Human Papillomavirus (Hpv) Vaccine Among African Americans And African Immigrants Young Adults, Adebola Adegboyega, Omoadoni Roseline Obielodan, Amanda T. Wiggins, Mark Dignan, Lovoria B. Williams

UK CARES Faculty Publications

Background Despite the disparate human papillomavirus (HPV) infection rates among sexually active Black young adults, HPV vaccine uptake remains low among this population. This study aimed to explore HPV beliefs, attitudes, and knowledge among Black young adults and provide recommendations on ways to improve vaccine uptake.

Methods We used a mixed-method, convergent design to conduct five focus groups and administered a 40-item electronic survey that was developed with health belief model (HBM) constructs. We assessed HPV and vaccine knowledge, barriers, and attitudes toward vaccination. We analyzed quantitative data using descriptive statistics and bivariate methods. Focus group transcripts were analyzed using …

Higher Concentrations Of Microplastics In Runoff From Biosolid-Amended Croplands Than Manure-Amended Croplands, Nasrin Naderi Beni, Shahab Karimifard, John Gilley, Tiffany L. Messer, Amy Schmidt, Shannon Bartelt-Hunt

UK CARES Faculty Publications

Land-applied municipal biosolids, produced from municipal wastewater treatment sludge, contributes to microplastics contamination in agroecosystems. The impacts of biosolids on microplastic concentrations in agricultural soil have been previously investigated, however, the potential for microplastics transport from biosolid-amended croplands has not been previously quantified. In this study, manure and biosolids were applied to field plots, runoff was collected following natural precipitation events and the potential of bacterial biofilm to grow on different microplastic morphologies was investigated. Higher concentrations of microplastics were detected in runoff from plots with land-applied biosolid in comparison with manure-amended and control plots. Fibers and fragments were the …

Novel Smad3 Variant Identified In A Patient With Familial Aortopathy Modeled Using A Zebrafish Embryo Assay, Mary Burchett Sheppard, Jeffrey D. Smith, Lisa L. Bergmann, Jakub Konrad Famulski

Saha Cardiovascular Research Center Faculty Publications

In human, pathogenic variants in smad3 are one cause of familial aortopathy. We describe a novel SMAD3 variant of unknown significance (VUS), V244F, in a patient who presented with aortic root dilation, right coronary artery ectasia, abdominal aortic aneurysm, right vertebral artery atresia, and cavernoma. Determination of variant pathogenicity impacted multiple aspects of the patient’s care, including the most appropriate surgical threshold for which to recommend a valve-sparing aortic root replacement. To determine whether the newly identified SMAD3 variant, and whether SMAD3 induced aortopathy in general, can be assayed in a zebrafish embryo model, we injected smad3a mRNA into Tg[kdrl:mCherry] …

Aortic Stress Activates An Adaptive Program In Thoracic Aortic Smooth Muscle Cells That Maintains Aortic Strength And Protects Against Aneurysm And Dissection In Mice, Chen Zhang, Yanming Li, Abhijit Chakraborty, Yang Li, Kimberly R. Rebello, Pingping Ren, Wei Luo, Lin Zhang, Hong S. Lu, Lisa A. Cassis, Joseph S. Coselli, Alan Daugherty, Scott A. Lemaire, Ying H. Shen

Saha Cardiovascular Research Center Faculty Publications

BACKGROUND: When aortic cells are under stress, such as increased hemodynamic pressure, they adapt to the environment by modifying their functions, allowing the aorta to maintain its strength. To understand the regulation of this adaptive response, we examined transcriptomic and epigenomic programs in aortic smooth muscle cells (SMCs) during the adaptive response to AngII (angiotensin II) infusion and determined its importance in protecting against aortic aneurysm and dissection (AAD).

METHODS: We performed single-cell RNA sequencing and single-cell sequencing assay for transposase-accessible chromatin (scATAC-seq) analyses in a mouse model of sporadic AAD induced by AngII infusion. We also examined the direct …

Gasdermin D Deficiency In Vascular Smooth Muscle Cells Ameliorates Abdominal Aortic Aneurysm Through Reducing Putrescine Synthesis, Jianing Gao, Yanghui Chen, Huiqing Wang, Xin Li, Ke Li, Yangkai Xu, Xianwei Xie, Yansong Guo, Nana Yang, Xinhua Zhang, Dong Ma, Hong S. Lu, Ying H. Shen, Yong Liu, Jifeng Zhang, Y. Eugene Chen, Alan Daugherty, Dao Wen Wang, Lemin Zheng

Saha Cardiovascular Research Center Faculty Publications

Abdominal aortic aneurysm (AAA) is a common vascular disease associated with significant phenotypic alterations in vascular smooth muscle cells (VSMCs). Gasdermin D (GSDMD) is a pore-forming effector of pyroptosis. In this study, the role of VSMC-specific GSDMD in the phenotypic alteration of VSMCs and AAA formation is determined. Single-cell transcriptome analyses reveal Gsdmd upregulation in aortic VSMCs in angiotensin (Ang) II-induced AAA. VSMC-specific Gsdmd deletion ameliorates Ang II-induced AAA in apolipoprotein E (ApoE)−/− mice. Using untargeted metabolomic analysis, it is found that putrescine is significantly reduced in the plasma and aortic tissues of VSMC-specific GSDMD deficient mice. High putrescine levels …

Clic And Membrane Wound Repair Pathways Enable Pandemic Norovirus Entry And Infection, B. Vijayalakshmi Ayyar, Khalil Ettayebi, Wilhelm Salmen, Umesh C. Karandikar, Frederick H. Neill, Victoria R. Tenge, Sue E. Crawford, Erhard Bieberich, B. V. Venkataram Prasad, Robert L. Atmar, Mary K. Estes

Markey Cancer Center Faculty Publications

Globally, most cases of gastroenteritis are caused by pandemic GII.4 human norovirus (HuNoV) strains with no approved therapies or vaccines available. The cellular pathways that these strains exploit for cell entry and internalization are unknown. Here, using nontransformed human jejunal enteroids (HIEs) that recapitulate the physiology of the gastrointestinal tract, we show that infectious GII.4 virions and virus-like particles are endocytosed using a unique combination of endosomal acidification-dependent clathrin-independent carriers (CLIC), acid sphingomyelinase (ASM)-mediated lysosomal exocytosis, and membrane wound repair pathways. We found that besides the known interaction of the viral capsid Protruding (P) domain with host glycans, the Shell …

Ifn-Γ Production By Brain-Resident Cells Activates Cerebral Mrna Expression Of A Wide Spectrum Of Molecules Critical For Both Innate And T Cell-Mediated Protective Immunity To Control Reactivation Of Chronic Infection With Toxoplasma Gondii, Yasuhiro Suzuki, Jenny Lutshumba, Kuey Chu Chen, Mohamed H. Abdelaziz, Qila Sa, Eri Ochiai

Markey Cancer Center Faculty Publications

We previously demonstrated that brain-resident cells produce IFN-g in response to reactivation of cerebral infection with Toxoplasma gondii. To obtain an overall landscape view of the effects of IFN-g from brain-resident cells on the cerebral protective immunity, in the present study we employed NanoString nCounter assay and quantified mRNA levels for 734 genes in myeloid immunity in the brains of T and B cell-deficient, bone marrow chimeric mice with and without IFN-g production by brain-resident cells in response to reactivation of cerebral T. gondii infection. Our study revealed that IFN-g produced by brain-resident cells amplified mRNA expression for the molecules …

Lignin Derived Hydrophobic Deep Eutectic Solvents As Sustainable Extractants, Yuxuan Zhang, Qi Qiao, Usman Lame Abbas, Jun Liu, Yi Zheng, Christopher Jones, Qing Shao, Jian Shi

Markey Cancer Center Faculty Publications

Solvent innovation has become a central task for improving the sustainability of chemical processes1. Deep eutectic solvents (DESs) emerge as environmentally friendly alternatives to toxic and volatile organic solvents. One appealing aspect for DESs is that they can be synthesized using naturally occurring compounds from biomass. Herein, we prepared novel hydrophobic DESs based on lignin derivatives and characterized their physicochemical properties including density, viscosity, and thermal behavior. The results showed that five lignin-derived hydrophobic DESs made from menthol, thymol, and 2,6-dimethoxyphenol were promising as green solvents due to their low viscosities and environmentally friendly constituents. To evaluate the potential application …

Production And Characterization Of Monoclonal Antibodies To Xenopus Proteins, Brett Horr, Ryan Kurtz, Ankit Pandey, Benjamin G. Hoffstrom, Elizabeth Schock, Carole Labonne, Dominique Alfandari

Markey Cancer Center Faculty Publications

Monoclonal antibodies are powerful and versatile tools that enable the study of proteins in diverse contexts. They are often utilized to assist with identification of subcellular localization and characterization of the function of target proteins of interest. However, because there can be considerable sequence diversity between orthologous proteins in Xenopus and mammals, antibodies produced against mouse or human proteins often do not recognize Xenopus counterparts. To address this issue, we refined existing mouse monoclonal antibody production protocols to generate antibodies against Xenopus proteins of interest. Here, we describe several approaches for the generation of useful mouse anti-Xenopus antibodies to multiple …

Grasses As Biofactories: Scoping Out The Opportunities, Nick Roberts, K. Richardson, G. Bryan, Christine R. Voisey, W. Mcnabb, T. Conner, M. Christey, R. Johnson

IGC Proceedings (1977-2023)

1. Plant biopharming is set to dominate commercial recombinant protein expression for specific proteins.
2. The choice of plant species depends on a multitude of factors and is determined on a case- by-case basis.
3. As a leaf based expression system grasses would have to compete predominantly with tobacco and alfalfa.
4. The grass-endophyte symbiosis offers a number of unique possibilities for biopharming.

Working Within Constraints: Managing African Savannas For Animal Production And Biodiversity, J. T. Du Toit

IGC Proceedings (1977-2023)

The mean density of livestock biomass on African rangelands now greatly exceeds that of indigenous large herbivores, although livestock cannot fully substitute for wildlife with respect to co-evolved ecosystem processes involving herbivory. The dominance of livestock in semi-arid rangelands is largely due to water provision, which uncouples livestock population dynamics from the rainfall-driven trajectories followed by indigenous ungulate species in wildlife areas. Ecological sustainability cannot be achieved with a few exotic species maintained at unprecedented biomass densities in savanna ecosystems, which are evolutionarily adapted for species-rich communities of ungulates of a wise range of sizes. Integrating wildlife and livestock in …

Review Of Storytelling In Queer Appalachia: Imagining The Unspeakable Other, Sandra L. Cotton Dnp, Rn, Anp-Bc, Fnap, Laurie A. Theeke Phd, James Messer

Journal of Appalachian Health

The Journal of Appalachian Health is committed to reviewing published media that relates to contemporary concepts affecting the health of residents of Appalachia. Improving the health in the region of Appalachia means knowing our people as they live and thrive in communities. The book reviewed here, Storytelling in Queer Appalachia: Imagining the Unspeakable Other (Edited by Glasby, Gradin, and Ryerson), is a must read for people who wish to gain insight on the real experience of being queer in Appalachia.

Carboxylic Acids And Light Interact To Affect Nanoceria Stability And Dissolution In Acidic Aqueous Environments, Matthew L. Hancock, Eric A. Grulke, Robert Yokel

Nanobiotechnology Center Faculty Publications

Cerium atoms on the surfaces of nanoceria (i.e., cerium oxide in the form of nanoparticles) can store or release oxygen, cycling between Ce³⁺ and Ce⁴⁺; therefore, they can cause or relieve oxidative stress within living systems. Nanoceria dissolution occurs in acidic environments. Nanoceria stabilization is a known problem even during its synthesis; in fact, a carboxylic acid, namely citric acid, is used in many synthesis protocols. Citric acid adsorbs onto nanoceria surfaces, limiting particle formation and creating stable dispersions with extended shelf life. To better understand factors influencing the fate of nanoceria, its dissolution and stabilization have …

An Intersectional Queer Case Study Of Two Youth Organizations In A Rural Kentucky High School, Eric Michael Moser

Theses and Dissertations--Community & Leadership Development

Secondary schools in the United States are hostile environments for students in the queer community. Schools in rural communities often pose greater challenges for queer students with less access to affirming and accepting educators. Rural schools often offer school-based agricultural education (SBAE) and the National FFA Organization as opportunities for students. Both programs have been found as unwelcoming environments for underrepresented groups, with little known about the experiences of queer students. To address hostility toward queer youth, schools have implemented Genders and Sexualities Alliances (GSAs) as an opportunity to discuss queer topics and create safe spaces. This qualitative case study …

Uky Slang Collection Project, Zada Komara

Lewis Honors College Research Data: Linguistics

The UKY Slang Collection Project is an ongoing effort (Fall 2021-present) to document the use of slang (group-specific language of informal register) primarily among college students at the University of Kentucky. The project is administered by Dr. Zada Komara (Lewis Honors College) as part of HON 140 Knowledge and Society, a foundational seminar about epistemology and ontology. This original research is performed by Lewis Honors College students. The project aims to document slang, both ephemeral and persistent, as a means of investigating students’ lived experiences of college, language evolution, and social identities among UKY student communities. Slang in the …

Community Supported Agriculture Values: A Comparison Across Groups, Thomas B. Pierce

Theses and Dissertations--Agricultural Economics

Community Supported Agriculture (CSA) programs offer consumers the chance to share the risk with farming operations while gaining access to fresh, local foods. While research on CSA shareholder values such as share affordability or the local impact of participation has been conducted in the past decade and beyond, less attention has been paid to direct value-mapping of the shareholders themselves. This research seeks to determine consumer values around the CSA purchasing decision using the Best-Worst Scaling Approach. Based on a focus group discussion with CSA shareholders in a university wellness voucher program, we used affinity diagramming to develop a list …

Addressing Money Laundering In The United States Real Estate Sector, Hunter Mccormick

Undergraduate Public Policy Capstone Projects

The United States faces threats to economic stability and legal integrity from money laundering practices in the real estate market. Legislative loopholes, complicit enablers, and aging technological systems used by government agencies allow individuals to flow ill-gotten wealth into the United States with little effort. As a result of these factors, local economies suffer job loss, once-inhabited properties lie empty in decay, and trust in government weakens. Illegal wealth is transformed into protected assets in the form of property and can stored to later fund terrorism organizations or influence the American people. Approximately $2.3 billion was laundered through real estate …

An Analysis Of The Clear Horizons Program In Perry County, Kentucky On Smoking Cessation, Julia Kollitz

Lewis Honors College Thesis Collection

Perry County is an Appalachian county located in southeastern Kentucky. The population is 28,473 people (United States Census Bureau, 2020). It is a predominately white community, with 95.9% of people falling in this category, which is higher than the national percentage of 75.8% (United States Census Bureau, 2020). Being a rural county, this community uses few news sources, with the primary outlet being WYMT Eastern Kentucky News. This news outlet is a subset of CBS (WYMT, 2023). When comparing statewide and nationwide data, Perry County does consistently worse. They have a high school graduation rate that is lower than the …

Exploring The Therapy Experiences Of Latinx Individuals Working With Non-Latinx Therapists, Edith Del Moral

Theses and Dissertations--Family Sciences

Latinos are the largest minoritized ethnic group in the United States. Despite having a population of 62.1 million Latinos in the United States, the representation of Latinx therapists remains low. Therefore, the chance for Latinx individuals and families to receive mental health services from a Latinx therapist is very low. This phenomenological study examines the experiences of six Latinx individuals who worked with non-Latinx therapists. The resulting themes included therapist client match, awareness, respect for boundaries, and genuineness. These themes informed by the personal stories of people who have gone through the therapeutic process will aid professionals in the field …

Social Ecological Factors And Medication Treatment For Opioid Use Disorder Among Justice-Involved Rural And Urban Persons: The Geographic Variation In Addiction Treatment Experiences (Gate) Longitudinal Cohort Study Protocol, Carrie B. Oser, Evan Batty, Marisa Booty, Kate Eddens, Hannah K. Knudsen, Brea Perry, Maria Rockett, Michele Staton

Sociology Faculty Publications

Introduction Three medications are Food and Drug Administration approved for the treatment of opioid use disorder (OUD); however, these medications are underused within prisons, which elevates the risk of relapse and overdose when persons with opioid use disorder (POUD) are released. Research is scant regarding the multilevel factors associated with POUDs’ willingness to initiate medication treatment for opioid use disorder (MOUD) while in prison and their continued engagement in treatment after release. Furthermore, rural and urban populations have not been compared. The Geographic variation in Addiction Treatment Experiences (GATE) study seeks to identify multilevel factors (ie, individual, personal network, and …

Effects Of Elevated Extracellular Dopamine On Astrocytes In The Nucleus Accumbens, Mengfan Xia

Theses and Dissertations--Pharmacology and Nutritional Sciences

Nucleus accumbens is involved in processing of motivational and affective behaviors and plays a significant role in development and maintenance of substance use. Released from the ventral tegmental area into the nucleus accumbens, the mesolimbic dopamine controls incentive salience and facilitates learning of reinforcement and reward-related motor functions. Recent studies reveal that, in the context of cocaine exposure, dopamine transmission in the ventromedial striatum is pivotal to the regulation of initial drug use. Astrocytes, a major subpopulation of the brain glial cells, are an active component of the so-called tripartite synapse and communicate with neurons through the release of transmitters …

Cardinal's Blues: Implementation Of A School-Based Cognitive Behavioral Therapy Program For Jefferson County Public Schools In Louisville, Kentucky, Loui Chang

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

With the increasing rate of adolescent depression and anxiety1 2, developing mental health interventions is necessary. Many adolescents’ times spent in schools increased compared to 20 years ago5, which makes school-based intervention programs suitable for reaching adolescent population effectively. Though there are numerous treatment providers available in Jefferson County, Kentucky, the school-based prevention programs addressing adolescent’s negative mental health outcomes have never been implemented. The Blueprints for Healthy Youth Development developed a program called the Blues Program24. The Blues Program provides an adapted version of group Cognitive Behavioral Therapy sessions to train and educate adolescents coping skills and cognitive restructuring24. …

"It's All Lateral Violence": How Sexual Minority Men Cope With Appearance Discrimination, Matthew T. Richardson

Theses and Dissertations--Educational, School, and Counseling Psychology

Sexual minority men experience higher rates of body dissatisfaction (BD) than heterosexual men (Frederick & Essayli, 2016). BD is associated with negative health outcomes in this population, including eating disorders (Yean et al., 2013), depression (Blashill et al., 2016), suicidality (Grunewald, Calzo, et al., 2021), and risky sexual behavior (Goedel et al., 2017). Sexual minority men who use dating apps may be at greater risk of experiencing BD via exposure to appearance-based discrimination (Tran et al., 2020), sexual objectification, and weight stigma (Filice et al., 2019). Little is known about sexual minority men’s experiences of appearance discrimination on dating apps …

Conserved Novel Interactions Between Post-Replicative Repair And Mismatch Repair Proteins Have Differential Effects On Dna Repair Pathways, Anna K. Miller

Theses and Dissertations--Toxicology and Cancer Biology

DNA mismatch repair (MMR) is the DNA repair mechanism that repairs base-base mispairs and small insertions and deletions remaining after replication. MMR is also required for apoptosis after certain types of exogenous DNA damage that result in damage-associated mispairs. The basic MMR mechanism is well understood; however, proteins associated with MMR continue to be identified. The roles of these interacting proteins in MMR are largely unknown. We have identified the yeast protein Rad5 as a novel interactor of the critical MMR proteins Msh2 and Mlh1. Rad5 is a DNA helicase and E3 ubiquitin ligase involved in post-replicative repair. However, to …

Beyond Mitosis, Plk1-Mediated Phosphorylation Re-Wires Cancer Metabolism And Promotes Cancer Progression, Qiongsi Zhang

Theses and Dissertations--Toxicology and Cancer Biology

Polo-like kinase 1 (PLK1) is a well- characterized regulator of cell division and is known to be highly expressed in certain types of tumors. It has been demonstrated the multifaceted roles of PLK1 in regulation of transcription, translation, epigenetics, DNA damage and cellular metabolism et al. Despite these findings, the precise mechanisms by which PLK1 regulates these processes beyond mitosis remain unclear. PLK1-mediated phosphorylation and misregulation of its substrates has been linked to tumorigenesis, cancer progression, drug resistance and worse prognosis. In this study, we investigated the non-canonical functions of PLK1 in cancer metabolism and drug resistance. We found that …

Disparities In Mental Health Technology Among African Americans: Breaking Cultural Inclusion Barriers, Nyoki Cosey-Brown

DSW Capstone Projects

Mental health technology is a healthcare delivery model of the future. The engagement in virtual healthcare was intensified when traditional healthcare methods were inaccessible during the federal restrictions implemented due to the COVID-19 pandemic. Utilizing digital apparatuses for healthcare improved the convenience, efficiency, and accessibility of various mental health services that were inoperable in past healthcare models. However, the historical constructs of stigma, discrimination, inequity, exclusion, and structural racism in mental healthcare were prevalent problems that remained present in virtual mental health platforms concerning the experiences of African-American patients and professionals who utilized the spaces.

This research explored mental health …

Substance Use And Trauma Among Adult Education Students In The United States, Brittany Miller-Roenigk, Michael Awad, Maria C. Crouch, Derrick M. Gordon

Educational, School, and Counseling Psychology Faculty Publications

Adult basic education (ABE) programs in the United States serve millions of students annually to help them achieve high school equivalency, English proficiency, and other skills. These skills are necessary for upward mobility and competitiveness in the labor market, which is important for ABE students who are disproportionately affected by racial/ethnic disparities and poverty. Among learners who are not in ABE programs, substance use and trauma affect student outcomes. Similar research is limited among ABE students. Understanding the influence of these factors among ABE students can better inform interventions. The current study, grounded in Stress and Coping Theory, examined rates, …

Writing New Normals: A Study Of American Pandemic Fiction In The Wake Of Covid-19, Riley E. Droppleman

Gaines Fellow Senior Theses

The purpose of this thesis is to explore the aspects of pandemic fiction that extend beyond the illness itself. In this thesis, I show that while the disease that spreads is a large focus of pandemic fiction, the truths that spring from these novels reveal larger societal issues, such as class hierarchies, racial inequalities, and failings of the government to fight the pandemic. In fact, pandemics act as catalysts for the uncovering of these societal ills, and the authors of pandemic fictions bring these greater faults of society to the forefront of their novels. I analyze three novels from the …

Restorative Landscapes And Their Potential Application To The University Of Kentucky Campus, Felix Lowery

Gaines Fellow Senior Theses

Exposure to natural environments has been shown to have significant positive impacts on both mood and attention, as well as the potential to decrease stress and other negative emotions. Hospitals and other healthcare settings have been on the forefront of implementing spaces designed with the intent of improving mental health. However, this is not the only setting in which restorative landscapes are needed. Implementing restorative landscapes on college campuses would be an innovative way for colleges to address their students’ mental health needs. The objective of this thesis is to understand what aspects of a natural landscape promote cognitive restoration, …

Cyborgs, Dolls And Passing Narratives: Trans-Femininity In Popular Music, Quinn J. Troia

Lewis Honors College Thesis Collection

The Cyborg is a figure that has been used by feminist scholars as a metaphor for feminist issues and transgender identity because the embodiment of both transgender people and cyborgs challenges binary understandings of male/femaleness and human/nonhumanness respectively. This comparison has also suggested the potential of reading cyborgs as passing figures who attempt to perform normative social identities; however, scholarship analyzing Cyborg figures has not explored this in ways that are specific to trans-feminine people. By combining contributions from the theory of gender performativity and research on transgender linguistic practices and identity construction, I perform a visual and textual analysis …