Digital Commons Network^™

Weighted Mean Difference Statistics For Paired Data In The Presence Of Missing Values, Yuntong Li, Brent J. Shelton, William St Clair, Heidi L. Weiss, John L. Villano, Arnold Stromberg, Chi Wang, Li Chen

Markey Cancer Center Faculty Publications

Missing data is a common issue in many biomedical studies. Under a paired design, some subjects may have missing values in either one or both of the conditions due to loss of follow-up, insufficient biological samples, etc. Such partially paired data complicate statistical comparison of the distribution of the variable of interest between the two conditions. In this article, we propose a general class of test statistics based on the difference in weighted sample means without imposing any distributional or model assumption. An optimal weight is derived from this class of tests. Simulation studies show that our proposed test with …

Technology Interaction And Policy Evolution; Grassland Society Of Southern Australia Inc, L. L. Bennison

IGC Proceedings (1977-2023)

The Grassland Society of Southern Australia was formed in 1959 to provide a forum for the transfer of information, ideas and experiences in relation to all aspects of grassland establishment, maintenance, utilisation, persistence and research. The membership of over 2,000 is composed primarily of primary producers (80%) with extension and research scientists comprising 10%, agribusiness 8% and academia 2%. The major interests of members are wool, lamb and beef production, dairying and cropping. The society produces bimonthly newsletters, holds an annual conference with attendances between 400 and 600 annually and has a branch structure that hosts local events. The society …

The Lotus Newsletter: An Electronic Lotus Research Community, Mónica Rebuffo

IGC Proceedings (1977-2023)

The Lotus Newsletter (LN) was created by Dr. W.F. Grant (former editor) in 1971, integrating scientists working on diverse aspects of research on Lotus spp. The LN received further impetus under the editorship of Dr. P.R. Beuselinck, who shaped the electronic version (http://www.psu.missouri.edu/lnl/). The newsletter aims to provide a vehicle for the exchange of information where opinions, in addition to established facts, can be presented. One of the strengths of the LN has been the lead article for each issue and the extensive bibliographic listing published every year, which facilitates access to recent literature. As a …

Citizen Scientists: Efforts By The Tri-Societies To Inject Science Into Us Policymaking, E. Bergfeld, K. Glasener

IGC Proceedings (1977-2023)

The Tri-Societies (American Society of Agronomy, Crop Science Society of America and Soil Science Society of America), the largest life science professional societies in the United States (US) dedicated to the agronomic, crop and soil sciences, encompass approximately 18,000 members globally and include approximately 14,000 certified professionals who are a direct conduit to the farming communities across the US. The Tri-Societies' US-based federal science policy programmes have been active for decades. There is a Washington, DC-based Director of Science Policy, Congressional Science Fellows (CSF) and Science Policy Interns.

Uk Libraries' "Wildcat Histories:" Preserving Student Activist Social Media Content, Ruth E. Bryan, Taylor C. Leigh, Emily B. Collier

Library Presentations

In this presentation, we describe the current web archiving program at the University of Kentucky Libraries; provide an overview of the "Wildcat Histories" activist student organization social media preservation project, funded by Project STAND; discuss the technical aspects of the "Wildcat Histories" project; and the project's current status and lessons learned.

A Mini-Review Of What Matters In The Management Of Nas, Is Esc The Best Care?, Enrique Gomez Pomar

Pediatrics Faculty Publications

As the use of opioids and polysubstance by pregnant women has increased over the years, there has also been a sharp increase in cases of neonatal abstinence syndrome (NAS). Classically, infants affected by NAS have been cared for in neonatal intensive care units resulting in an increase of healthcare expenditure and resource utilization as well as separation from the families. Consequently, the Eat, Sleep, and Console (ESC) tool was developed and promoted as a novel method that focuses on maternal/infant dyad during hospital stay while decreasing the use of pharmacological interventions and therefore decreasing the length of stay and healthcare …

Making Home: The Role Of Social Networks On Identity, Health, And Quality Of Life Among Rural Lesbian And Gay Older Adults, Marc A. Guest, Elizabeth G. Hunter, Nancy E. Schoenberg

UK CARES Faculty Publications

Background and Objectives: Gay and lesbian older persons face a host of health inequalities related to their identity as they age. Challenges to health access and appropriate social support may be even more exacerbated for those living in rural environments; this may be due to the lack of supportive and affirming social connections. This project aimed to explore and describe the social networks and the relationship of these social networks to identity, health, and quality of life of gay and lesbian individuals in rural communities.

Research Design and Methods: Social network data on network type, size, and social capital were …

Emt Activates Exocytotic Rabs To Coordinate Invasion And Immunosuppression In Lung Cancer, Guan-Yu Xiao, Xiaochao Tan, Bertha L. Rodriguez, Don L. Gibbons, Shike Wang, Chao Wu, Xin Liu, Jiang Yu, Mayra E. Vasquez, Hai T. Tran, Jun Xu, William K. Russell, Cara Haymaker, Younghee Lee, Jianjun Zhang, Luisa Solis, Ignacio I. Wistuba, Jonathan M. Kurie

Markey Cancer Center Faculty Publications

Epithelial-to- mesenchymal transition (EMT) underlies immunosuppression, drug resistance, and metastasis in epithelial malignancies. However, the way in which EMT orchestrates disparate biological processes remains unclear. Here, we identify an EMT- activated vesicular trafficking network that coordinates promigratory focal adhesion dynamics with an immunosuppressive secretory program in lung adenocarcinoma (LUAD). The EMT-activating transcription factor ZEB1 drives exocytotic vesicular trafficking by relieving Rab6A, Rab8A, and guanine nucleotide exchange factors from miR-148a-dependent silencing, thereby facilitating MMP14-dependent focal adhesion turnover in LUAD cells and autotaxin-mediated CD8+ T cell exhaustion, indicating that cell- intrinsic and extrinsic processes are linked through a microRNA that coordinates vesicular …

Messes: Software For Transforming Messy Research Datasets Into Clean Submissions To Metabolomics Workbench For Public Sharing, P. Travis Thompson, Hunter N. B. Moseley

Markey Cancer Center Faculty Publications

In recent years, the FAIR guiding principles and the broader concept of open science has grown in importance in academic research, especially as funding entities have aggressively promoted public sharing of research products. Key to public research sharing is deposition of datasets into online data repositories, but it can be a chore to transform messy unstructured data into the forms required by these repositories. To help generate Metabolomics Workbench depositions, we have developed the MESSES (Metadata from Experimental SpreadSheets Extraction System) software package, implemented in the Python 3 programming language and supported on Linux, Windows, and Mac operating systems. MESSES …

Nac1 Confines Virus‐Specific Memory Formation Of Cd4+ T Cells Through The Rock1‐Mediated Pathway, Liqing Wang, Hao-Yun Peng, Jugal Kishore Das, Anil Kumar, Yijie Ren, Darby J. Ballard, Xiaofang Xiong, Wen Yang, Xingcong Ren, Paul De Figueiredo, Jin-Ming Yang, Jianxun Song

Markey Cancer Center Faculty Publications

Nucleus accumbens‐associated protein 1 (NAC1), a transcriptional cofactor, has been found to play important roles in regulating regulatory T cells, CD8 + T cells, and antitumor immunity, but little is known about its effects on T‐cell memory. In this study, we found that NAC1 expression restricts memory formation of CD4 + T cells during viral infection. Analysis of CD4 + T cells from wild‐type (WT) and NAC1‐ deficient (−/−) mice showed that NAC1 is essential for T‐cell metabolism, including glycolysis and oxidative phosphorylation, and supports CD4 + T‐cell survival in vitro. We further demonstrated that a deficiency of NAC1 downregulates …

Herbage Production Of Tanzania Grass (Panicum Maximum Cv. Tanzania) Submitted To Combinations Of Frequencies And Intensities Of Grazing By Cattle, Domicio Nascimento Jr., Rodrigo A. Barbosa, Valéria P. B. Euclides, S. C. Da Silva, R. A. Torres

IGC Proceedings (1977-2023)

Animal production from pastures is a complex process comprising three main stages: herbage growth, consumption by grazing animals and conversion into animal products (Hodgson, 1990). Utilisation is the stage where the grazier finds greater flexibility for management, probably because most processes related to harvest of the produced herbage by the grazing animals are very responsive to manipulation and control of defoliation practices. The objective of this study was to evaluate herbage production of a Panicum maximum cv. Tanzania pasture submitted to combinations of frequencies and intensities of grazing.

A New Herbicide (Gf-839) For Long-Term Control Of Annual And Perennial Broad-Leaved Weeds In Grassland, S. A. Egerton, A. D. Bailey, Louise A. Brinkworth

IGC Proceedings (1977-2023)

The new herbicide GF-839 is a combination of a new active substance aminopyralid and the fully approved active substance fluroxypyr in the quantities 30 g ae/l aminopyralid + 100 g ae/l fluroxypyr. It is an emulsion, water in oil formulation (EO), and will be sold as a foliar acting herbicide for the long-term control of annual and perennial broad-leaved weeds in grassland. Globally aminopyralid can be used for weed control in range and pasture situations and plantations; in addition, uses in oilseed rape and cereals are also being explored. Aminopyralid is the most active halopyridine yet discovered and as a …

Are Leaf Traits Suitable For Assessing The Feeding Value Of Native Grass Species?, R. Al Haj Khaled, M. Duru, P. Cruz

IGC Proceedings (1977-2023)

Research on forage feeding value other than in vivo assessment can be roughly divided into three kinds of approach. The first aims to predict feeding value using a set of enzymatic or physical methods. A second approach is based on phenological stages of species. These approaches are mainly used for pure stands of improved grasses or legumes. However, for native grassland, a complex type of vegetation, a third approach, based on botanical records, has been proposed to rank grassland communities for their feeding value. The aim of this work concerns the third approach. We tested whether leaf traits (e.g. specific …

Will Our Future Selves Thank Us? An Examination Of Born-Digital Curation Practices At Ukl, Megan M. Mummey, Sarah Dorpinghaus, Ruth Bryan, Emily Collier

Library Presentations

Cultural heritage resources are increasingly being produced and distributed digitally yet the world of physical materials has not declined. Analog-based archival theory and practice is still relevant, but born-digital formats make acquisition, appraisal, resource allocation, collection management, and external relationships much more challenging. During this session, four archivists and librarians from the University of Kentucky Libraries (UKL) will share their current challenges and successes working with different aspects of born-digital stewardship. They will share practical tips, tools, policies, and mental frameworks to help attendees identify gaps, pitfalls, and opportunities in digital stewardship at their own institutions. Sarah Dorpinghaus will provide …

Preservation Perseverance: Archiving Social Media Content, A University Of Kentucky/Latino Student Union Collaboration, Ruth E. Bryan, Taylor C. Leigh, Emily B. Collier

Library Presentations

The voices of activist student leaders and organizations are crucial to preserve, whether it be in a formal archives or as part of an archiving process undertaken by individuals and groups to preserve their legacies. Increasingly, these voices are found in social media and other online and web platforms that are difficult to preserve. Wildcat Histories is a year-long, grant-funded collaboration between the University of Kentucky Libraries and the University of Kentucky Latino Student Union (LSU) to research the best technologies for (as of July 2023) and then carry out the preservation of activist student organization social media content. The …

V2g Optimization For Dispatchable Residential Load Operation And Minimal Utility Cost, Rosemary E. Alden, Ashutosh Timilsina, Simone Silvestri, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

Electric Vehicles (EVs) are gaining popularity among consumers and are expected to play a significant role in the future of transportation. Within this paper, a reverse auction is formulated through an optimization problem to minimize the utility energy cost using Vehicle-to-Grid (V2G) operation, as well as transition residential communities to dispatchable aggregate constant load profiles for demand response (DR). The evolution-ary V2G Auction (eV2GA), including the non-dominated sorting genetic algorithm (NSGA-II), is proposed for the formulated problem. It uses co-simulation with OpenDSS for power flow analysis as part of the objective function to account for physical constraints of infrastructure on …

Optimization Of An Electric Vehicle Traction Motor With A Pm Flux Intensifying Stator And A Reluctance Outer Rotor, Oluwaseun A. Badewa, Ali Mohammadi, Donovin Lewis, Dan Ionel, Somasundaram Essakiappan, Madhav Manjrekar

Electrical and Computer Engineering Graduate Research

This paper proposes a novel electric motor concept using stator-embedded phase coils and permanent magnets (PM). A unique pattern of phase coil placement in separate slots using concentrated windings is employed. High flux intensification is achieved using low remanence and non-rare-earth permanent magnets in a spoke-type field arrangement. The exterior rotor has no active electromagnetic component thereby achieving a simple reluctance type configuration. The principle of operation is based on the concept of virtual work with closed-form analytical airgap flux density distributions. Preliminary parametric design studies followed by large scale multi-objective optimization were carried out using electromagnetic FEA to study …

Axial Flux Permanent Magnet Vernier Machine With Single-Wound Dual-Stator And Spoke Permanent Magnet Rotor For Electric Vehicle In-Wheel Traction, Ali Mohammadi, Yaser Chulaee, Aaron M. Cramer, Ion G. Boldea, Dan M. Ionel

Electrical and Computer Engineering Graduate Research

In this paper, a dual-stator axial flux permanent magnet machine with a spoke-type rotor for electric vehicle applications is investigated. The rotor PMs are positioned in a spoke-type field-concentrating arrangement, which provides high flux concentration. Two stators—one stator configuration with double-layer wound coils and another without winding—are employed. The usage of single-layer wound coils is possible in this topology, and by using this type of winding it is possible to implement advanced in-slot cooling systems. Analytical and electromagnetic finite element analysis (FEA) studies considering its geometrical and electrical properties have been performed on this machine. The performance indices for the …

Optimal Power Sharing Speed Compensation In On-Road Wireless Ev Charging Systems, Donovin Lewis, Omer Onar, Veda Prakash Galigekere, Mostak Mohammad, Dan Ionel

Electrical and Computer Engineering Graduate Research

Dynamic wireless charging of electric vehicles (EV) is an emerging technology with the potential to address range anxiety and reduce the size of batteries or provide charge sustaining operation. Charging demand for dynamic wireless charging systems (DWCS) varies greatly in response to location specific traffic behaviors including the number and speed of vehicles. This paper highlights the potential reduction of load variation with speed compensation and simulates opportunities to maximize the number of cars charged concurrently through ”power sharing” or altering power output to slower cars while maintaining a maximum delivered energy. An improved sub- minute model for synthetic traffic …

Realizing Innovation And Sustainability: A Case Study Of Macau Sar’S Smart City Development Capabilities, Yujia He, Felix Ter Chian Tan, Carmen Leong, Jingbo Huang, Don Rodney Junio

Patterson School of Diplomacy and International Commerce Faculty Publications

The emergence of digital technologies has transformed organizational innovation with important implications for a city, its economy and policymaking. Despite this, research on how capabilities underpin innovation through smart city initiatives is still lacking. To address this gap, we conduct an empirical investigation of smart city initiatives in Macau, a former Portuguese colony and now special administrative region (SAR) of China. The case study adopts a qualitative approach, using data collected through fieldwork observations and in-depth interviews with stakeholders, and analysis of secondary documents. The study develops a stage-based assessment of innovation capabilities related to the use of technologies in …

Creation Of Egd-Derived Gastric Cancer Organoids To Predict Treatment Responses, Hannah Mcdonald, Megan Harper, Kristen S. Hill, Anqi Gao, Angelica L. Solomon, Charles J. Bailey, Miranda Lin, Mautin Barry-Hundeyin, Michael Cavnar, Samuel H. Mardini, Prakash Pandalai, Reema A. Patel, Jill M. Kolesar, Justin A. Rueckert, Lawrence Hookey, Mark Ropeleski, Shaila J. Merchant, Joseph Kim, Mei Gao

Markey Cancer Center Faculty Publications

Gastric adenocarcinoma (GAd) is the third leading cause of cancer-related deaths worldwide. Most patients require perioperative chemotherapy, yet methods to accurately predict responses to therapy are lacking. Thus, patients may be unnecessarily exposed to considerable toxicities. Here, we present a novel methodology using patient-derived organoids (PDOs) that rapidly and accurately predicts the chemotherapy efficacy for GAd patients.

Methods: Endoscopic GAd biopsies were obtained from 19 patients, shipped overnight, and PDOs were developed within 24 h. Drug sensitivity testing was performed on PDO single-cells with current standard-of-care systemic GAd regimens and cell viability was measured. Whole exome sequencing was used to …

Differential Inhibition Of Anaplerotic Pyruvate Carboxylation And Glutaminolysis-Fueled Anabolism Underlies Distinct Toxicity Of Selenium Agents In Human Lung Cancer, Teresa W-M Fan, Jason Winnike, Ahmad Al-Attar, Alex C. Belshoff, Pawel Lorkiewicz, Jin Lian Tan, Min Wu, Richard M. Higashi, Andrew N. Lane

Markey Cancer Center Faculty Publications

Past chemopreventive human trials on dietary selenium supplements produced controversial outcomes. They largely employed selenomethionine (SeM)-based diets. SeM was less toxic than selenite or methylseleninic acid (MSeA) to lung cancer cells. We thus investigated the toxic action of these Se agents in two non-small cell lung cancer (NSCLC) cell lines and ex vivo organotypic cultures (OTC) of NSCLC patient lung tissues. Stable isotope-resolved metabolomics (SIRM) using 13C6-glucose and 13C5,15N2-glutamine tracers with gene knockdowns were employed to examine metabolic dysregulations associated with cell type- and treatment-dependent phenotypic changes. Inhibition of key anaplerotic processes, pyruvate carboxylation (PyC) and glutaminolysis were elicited by …

Identifying And Sharing Per-And Polyfluoroalkyl Substances Hot-Spot Areas And Exposures In Drinking Water, Sweta Ojha, P. Travis Thompson, Christian D. Powell, Hunter N. B. Moseley, Kelly G. Pennell

Markey Cancer Center Faculty Publications

Exposure to per- and polyfluoroalkyl substances (PFAS) in drinking water is widely recognized as a public health concern. Decision-makers who are responsible for managing PFAS drinking water risks lack the tools to acquire the information they need. In response to this need, we provide a detailed description of a Kentucky dataset that allows decision-makers to visualize potential hot-spot areas and evaluate drinking water systems that may be susceptible to PFAS contamination. The dataset includes information extracted from publicly available sources to create five different maps in ArcGIS Online and highlights potential sources of PFAS contamination in the environment in relation …

Novel Isolation Method Reveals Sex-Specific Composition And Neurotoxicity Of Small Extracellular Vesicles In A Mouse Model Of Alzheimer’S Disease, Ahmed Elsherbini, Zhihui Zhu, Zainuddin Quadri, Simone M. Crivelli, Xiaojia Ren, Hemendra J. Vekaria, Priyanka Tripathi, Liping Zhang, Wenbo Zhi, Erhard Bieberich

Markey Cancer Center Faculty Publications

We developed a new method to isolate small extracellular vesicles (sEVs) from male and female wild-type and 5xFAD mouse brains to investigate the sex-specific functions of sEVs in Alzheimer’s disease (AD). A mass spectrometric analysis revealed that sEVs contained proteins critical for EV formation and Aβ. ExoView analysis showed that female mice contained more GFAP and Aβ-labeled sEVs, suggesting that a larger proportion of sEVs from the female brain is derived from astrocytes and/or more likely to bind to Aβ. Moreover, sEVs from female brains had more acid sphingomyelinase (ASM) and ceramide, an enzyme and its sphingolipid product important for …

Physical Impediment Towards Digestive Breakdown In Leaf Blades Of Brachiaria Brizantha, B. Lempp, Cacilda B. Do Valle, M. Das G. Morais, R. A. Borges, E. Detmann

IGC Proceedings (1977-2023)

Consumption of grasses is influenced by the physical properties of forages which confer resistance to digestive breakdown. Such barriers may be the proportion of indigestible tissues, girder structure and epidermal cell arrangements. Anatomical factors, if identified early are invaluable tools in breeding and selection programmes for forages of high quality. The objective of this study was to verify which anatomical attributes might be interfering in the physical resistance to rumen breakdown in Brachiaria brizantha ecotypes.

Are Leaf Traits Stable Enough To Rank Native Grasses In Contrasting Growth Conditions?, V. Poozesh, R. Al Haj Khaled, P. Ansquer, J. P. Theau, M. Duru, G. Bertoni, P. Cruz

IGC Proceedings (1977-2023)

The growing interest in classifying species in response groups relating to variations in environmental factors has triggered the search for functional traits that express differences in ecological behaviour among plant species (Lavorel & Garnier, 2002). Specific leaf area (SLA) and leaf dry matter content (LDMC) reflect a fundamental trade-off in plant functioning between a fast growth rate (high SLA, low LDMC species) and nutrient conservation (low SLA, high LDMC species). This study aimed to analyse the stability of ranking native grasses by SLA and LDMC values under different plant growing conditions.

Effect Of Three Legumes Containing Different Condensed Tannin Concentrations On The In Vitro Formation Of The Pastoral Flavour Compound; Skatole, N. M. Schreurs, M. H. Tavendale, G. A. Lane, T. N. Barry, W. C. Mcnabb

IGC Proceedings (1977-2023)

Feeding legumes, such as white clover (Trifolium repens), results in higher intakes and increased animal production compared to grasses (Ulyatt, 1981). Skatole is produced in the rumen from plant protein fermentation and is associated with undesirable pastoral flavours in meat (Young et al. 2002). Feeding white clover causes a greater skatole concentration in the rumen compared to perennial ryegrass (Lolium perenne) or Lotus corniculatus, as the protein in white clover is highly soluble and rapidly degraded (Schreurs et al., 2004). The condensed tannins (CT) in Lotus species slow protein degradation in the rumen (Aerts …

The Last Of Us In Therapy: How Mind-Controlling Fungi And Gut Bacteria Affect Your Mental Health, Anastasia Lyon

Journal of Pharmacology & Nutritional Sciences

The "psilocybiome" represents the mutually beneficial relationship between ourselves, our bacteria, and psychedelic drugs. This short review briefly discusses the benefits and limitations surrounding the potential for psychedelic therapy to synergize with gut bacteria to help regulate and maintain proper balance in the immune system, diet, and stress levels. Psychedelic therapy is a novel treatment strategy that has the potential to improve patient mental health, and, by identifying the types of gut bacteria present in patients, it can aid in personalizing medicine by determining how well their "psilocybiome" may respond.

Torque And Power Capabilities Of Coreless Axial Flux Machines With Surface Pms And Halbach Array Rotors, Yaser Chulaee, Donovin Lewis, Matin Vatani, John F. Eastham, Dan Ionel

Power and Energy Institute of Kentucky Faculty Publications

This paper investigates employing a Halbach PM rotor array to increase torque and power density within coreless axial flux permanent magnet (AFPM) machines. Analytical and 2/3-dimensional finite element analysis (FEA) methods are developed to study torque and power capabilities within an example double-rotor, single-stator coreless AFPM machine with a PCB stator. Compared to a surface PM topology of the same mass and volume, employing a Halbach array increases torque density by as much as 30% through increased airgap flux density amplitude. Multiple parametric studies are performed to explore methods of increasing torque and power density while employing Halbach arrays combined …

Design Optimization Of A Direct-Drive Wind Generator With Non-Rare-Earth Pm Flux Intensifying Stator And Reluctance Rotor, Ali Mohammadi, Oluwaseun A. Badewa, Yaser Chulaee, Dan Ionel, Somasundaram Essakiappan, Madhav Manjrekar

Power and Energy Institute of Kentucky Faculty Publications

This paper presents a multi-objective design optimization for a novel direct-drive wind turbine generator. The proposed electric machine topology employs an outer rotor of the reluctance type and a special modular stator including three phase-windings and spoke-type permanent magnets (PMs). Each stator module includes a single coil toroidally wound around the ferromagnetic core. Consecutive stator modules are separated by PMs and include coils belonging to a different phase. An optimization method with three objectives: total power loss, weight, and torque ripple, and with one constraint for a minimum acceptable value for the power factor, is described. The design examples are …