Digital Commons Network^™

Resistance Exercise And Mechanical Overload Upregulate Vimentin For Skeletal Muscle Remodeling, Joshua S. Godwin, J. Max Michel, Cleiton A. Libardi, Andreas N. Kavazis, Christopher S. Fry, Andrew D. Frugé, Mariah Mccashland, Ivan J. Vechetti, John J. Mccarthy, C. Brooks Mobley, Michael D. Roberts

Physiology Faculty Publications

We adopted a proteomic and follow-through approach to investigate how mechanical overload (MOV) potentially affects novel targets in skeletal muscle, and how a perturbation in this response could potentially affect the adaptive response. First, we determined that 10 wk of resistance training in 15 college-aged females increased sarcolemmal-associated protein content (+10.1%, P < 0.05). Sarcolemmal protein isolates were then queried using mass spectrometry-based proteomics, ∼10% (38/387) of proteins putatively associated with the sarcolemma or extracellular matrix (ECM) were upregulated (>1.5-fold, P < 0.05), and one target (intermediate filament vimentin; VIM) warranted further investigation due to its correlation to myofiber hypertrophy (r = 0.652, P = 0.009). VIM expression was then examined in 4-mo-old C57BL/6J mice following 10 and 20 days of plantaris MOV via synergist ablation. Relative to Sham (control) mice, VIM mRNA and protein content was significantly higher in MOV mice, and immunohistochemistry indicated that VIM predominantly resided in the ECM. MOV experiments were replicated in Pax7-DTA (satellite cell depleted) mice, which reduced VIM in the ECM by ∼74%. A third MOV experiment was performed in C57BL/6 mice intramuscularly injected with either AAV9-scrambled (control) or AAV9-VIM-shRNA. Although VIM-shRNA mice possessed lower VIM in the ECM (∼45%), plantaris masses in response to MOV were similar between groups. However, VIMshRNA mice possessed smaller and more centrally nucleated MyHCemb-positive fibers in response to MOV. In summary, skeletal muscle VIM appears to be enriched in the ECM following MOV, satellite cells may regulate its expression, and a disruption in expression during MOV leads to an excessive regenerative phenotype.

Therapeutic Applications Of A Novel Humanized Monoclonal Antibody Targeting Chemokine Receptor Ccr9 In Pancreatic Cancer, Hannah G. Mcdonald, Anna M. Reagan, Charles J. Bailey, Mei Gao, Muqiang Gao, Angelica L. Solomon, Michael J. Cavnar, Prakash Pandalai, Mautin Barry-Hundeyin, Megan Harper, Justin A. Rueckert, Ángela Turrero, Araceli Tobio, Anxo Vidal, Daniel Roca-Lema, Elia Álvarez-Coiradas, Pablo Garrido, Laureano Simón, Joseph Kim

Markey Cancer Center Faculty Publications

The relative failure of immune checkpoint inhibitors in pancreatic ductal adenocarcinoma (PDAC) despite having a dense, immunosuppressive tumor microenvironment highlights the need to target alternate/escape pathways. We have previously examined C–C chemokine receptor type 9 (CCR9) as a candidate immune checkpoint and developed a targeted, humanized monoclonal antibody (SRB2). Cytotoxicity of SRB2 was evaluated in vitro and in vivo. CCR9 expression on PDAC cells/tissues, immune components of patient-derived organoids (PDOs), and antibody-dependent cell-mediated cytotoxicity were examined. In PANC-1 and MIA PaCa-2 cell lines, we demonstrated highest CCR9 expression; however, no direct cytotoxic effect was observed with SRB2 treatment. In PANC-1 …

An Agrin-Yap/Taz Rigidity Sensing Module Drives Egfr-Addicted Lung Tumorigenesis., Reza Bayat Mokhtari, Divyaleka Sampath, Paige Eversole, Melissa Ong Yu Lin, Dmitriy A. Bosykh, Gandhi T. K. Boopathy, Aravind Sivakumar, Cheng-Chun Wang, Ramesh Kumar, Joe Yeong Poh Sheng, Ellen Karasik, Barbara A. Foster, Han Yu, Xiang Ling, Wenjie Wu, Fengzhi Li, Zoë Weaver Ohler, Christine Fillmore Brainson, David W. Goodrich, Wanjin Hong, Sayan Chakraborty

Markey Cancer Center Faculty Publications

Despite epidermal growth factor receptor (EGFR) is a pivotal oncogene for several cancers, including lung adenocarcinoma (LUAD), how it senses extracellular matrix (ECM) rigidity remain elusive in the context of the increasing role of tissue rigidity on various hallmarks of cancer development. Here it is shown that EGFR dictates tumorigenic agrin expression in lung cancer cell lines, genetically engineered EGFR-driven mouse models, and human specimens. Agrin expression confers substrate stiffness-dependent oncogenic attributes to EGFR-reliant cancer cells. Mechanistically, agrin mechanoactivates EGFR through epidermal growth factor (EGF)-dependent and independent modes, thereby sensitizing its activity toward localized cancer cell-ECM adherence and bulk rigidity …

Temporal Immune Profiling In The Cerebrospinal Fluid And Blood Of Patients With Aneurysmal Subarachnoid Hemorrhage, Thomas A. Ujas, Katie L. Anderson, Jenny Lutshumba, Samantha N. Hart, Jadwiga Turchan-Cholewo, Kevin W. Hatton, Adam D. Bachstetter, Barbara S. Nikolajczyk, Ann M. Stowe

Surgery Faculty Publications

Delayed cerebral ischemia (DCI) is a significant complication of aneurysmal subarachnoid hemorrhage (aSAH). This study profiled immune responses after aSAH and evaluated their association with DCI onset. Twelve aSAH patients were enrolled. Leukocyte populations and cytokine levels were analyzed in cerebrospinal fluid (CSF) and peripheral blood (PB) on days 3, 5, 7, 10, and 14 post-aSAH. PB mononuclear cells (PBMCs) were collected, and their cytokine production quantified following stimulation. Mixed-effects models reveal distinct immune cell dynamics in CSF compared with blood. Monocyte/macrophage numbers continue to increase in both CSF and PBMCs for days post-aSAH. CD4⁺ human leukocyte antigen II+ …

Training, Validating, And Testing Machine Learning Prediction Models For Endometrial Cancer Recurrence, Jesus Gonzalez Bosquet, Andrew Polio, Erin George, Ahmad A. Tarhini, Casey M. Cosgrove, Marilyn S. Huang, Bradley Corr, Aliza L. Leiser, Bodour Salhia, Kathleen Darcy, Christopher M. Tarney, Rob L. Dood, Lauren E. Dockery, Stephen B. Edge, Michael J. Cavnar, Lisa Landrum, Rob J. Rounbehler, Michelle Churchman, Vincent M. Wagner

Surgery Faculty Publications

PURPOSEEndometrial cancer (EC) is the most common gynecologic cancer in the United States with rising incidence and mortality. Despite optimal treatment, 15%-20% of all patients will recur. To better select patients for adjuvant therapy, it is important to accurately predict patients at risk for recurrence. Our objective was to train, validate, and test models of EC recurrence using lasso regression and other machine learning (ML) and deep learning (DL) analytics in a large, comprehensive data set.METHODSData from patients with EC were downloaded from the Oncology Research Information Exchange Network database and stratified into low risk, The International Federation of Gynecology …

Shared Roles Of Immune And Stromal Cells In The Pathogenesis Of Human Bronchiolitis Obliterans Syndrome, Patrick W. Mellors, Ana N. Lange, Bruno Casino Remondo, Maksim Shestov, Joseph D. Planer, Andrew R. Peterson, Yun Ying, Su Zhou, Jason D. Christie, Joshua M. Diamond, Edward Cantu, Maria C. Basil, Saar Gill

Surgery Faculty Publications

Bronchiolitis obliterans syndrome (BOS) is a progressive, fatal obstructive lung disease that occurs following lung transplant, where it is termed chronic lung allograft dysfunction BOS (CLAD-BOS), or as the primary manifestation of pulmonary chronic graft versus host disease (cGVHD-BOS) following allogeneic hematopoietic stem cell transplant. Disease pathogenesis is poorly understood; however, chronic alloreactivity is common to both conditions, suggesting a shared pathophysiology. We performed single-cell RNA-Seq (scRNA-Seq) on explanted human lungs from 4 patients with CLAD-BOS, 3 patients with cGVHD-BOS, and 3 deceased controls to identify cell types, genes, and pathways enriched in BOS to better understand disease mechanisms. In …

Reproductive Performance And Milk Composition Of Sows Fed Diets Supplemented With An Immunomodulator, Mark J. Estienne, Jung W. Lee, R. Tyler Niblett, Brooke D. Humphrey, H. James Monegue, Merlin D. Lindemann

Animal and Food Sciences Faculty Publications

A cooperative study involving 189 litters from 114 sows (initial BW of 200.8 ± 37.1 kg) at two experiment stations was conducted to investigate the effects of dietary supplementation with OmniGen-AF (OG) (Phibro Animal Health Co., Teaneck, NJ, USA), a nutritional product formulated to improve immune function of animals, on sow reproductive performance and milk composition. Dietary treatments were (1) corn–soybean meal-based control diets or (2) control diets supplemented with OG at 0.75% (~9 g of OG/100 kg BW/d). Supplementation of diets with OG resulted in lesser (p < 0.05) BW changes of sows during lactation (−12.1 vs. −8.2 kg). Litter sizes for control and OG-fed sows were similar, but sows fed OG-based diets had greater (p < 0.05) litter weight for total born (18.3 vs. 19.3 kg) and weaned (63.2 vs. 67.0 kg) and lactation litter gain (47.8 vs. 50.7 kg). Lactation feed intake for the controls and OG-fed sows (5.32 vs. 5.52 kg/d, respectively) did not differ. Supplementing diets with OG increased lactose content (5.78 vs. 5.84%; p = 0.05) and reduced protein content (4.77 vs. 4.68%; p = 0.04) in sow milk. In conclusion, dietary supplementation with OG at 0.75% reduced weight loss during lactation and improved litter weights with marginal effects on the milk composition of sows.

Pparα Regulates Er–Lipid Droplet Protein Calsyntenin-3Β To Promote Ketogenesis In Hepatocytes, Lauren F. Uchiyama, Alexander Nguyen, Kevin Qian, Liujuan Cui, Khoi T. Pham, Xu Xiao, Yajing Gao, Yuta Shimanaka, Marcus J. Tol, Laurent Vergnes, Karen Reue, Peter Tontonoz

Physiology Faculty Publications

Ketogenesis requires fatty acid flux from intracellular (lipid droplets) and extrahepatic (adipose tissue) lipid stores to hepatocyte mitochondria. However, whether interorganelle contact sites regulate this process is unknown. Recent studies have revealed a role for Calsyntenin-3β (CLSTN3β), an endoplasmic reticulum–lipid droplet contact site protein, in the control of lipid utilization in adipose tissue. Here, we show that Clstn3b expression is induced in the liver by the nuclear receptor PPARα in settings of high lipid utilization, including fasting and ketogenic diet feeding. Hepatocyte-specific loss of CLSTN3β in mice impairs ketogenesis independent of changes in PPARα activation. Conversely, hepatic overexpression of CLSTN3β …

Consumer Perceptions Of Hemp-Fed Aquaculture, Jillian Hyink, Brandon R. Mcfadden, Saroj Adhikari, Brandy E. Phipps, Tyler Mark, Waldemar Rossi, Craig Schluttenhofer, Seong D. Yun

Agricultural Economics Faculty Publications

Global demand for seafood has increased significantly in recent decades and is projected to continue rising. Aquaculture production has increased in the United States and globally to meet this demand, offering a relatively sustainable supplement to wild fish supplies. However, increased production further strains natural populations of pelagic species used to make fishmeal and fish oil into feedstocks, raising production costs and introducing safety concerns associated with fish consumption. A potential alternative feed ingredient is hemp, which can provide a cost-effective and nutritious feed for carnivorous fish. Moreover, hemp could meet the requirements for the final product to carry a …

Clustering Individuals Using Inmtd, Zuqi Li, Sam F.L. Windels, Noël Malod-Dognin, Seth M. Weinberg, Mary L. Marazita, Susan Walsh, Mark D. Shriver, David W. Fardo, Peter Claes, Nataša Przulj, Kristel Van Steen

Biostatistics Faculty Publications

Motivation: Combining omics and images can lead to a more comprehensive clustering of individuals than classic single-view approaches. Among the various approaches for multi-view clustering, nonnegative matrix tri-factorization (NMTF) and nonnegative Tucker decomposition (NTD) are advantageous in learning low-rank embeddings with promising interpretability. Besides, there is a need to handle unwanted drivers of clusterings (i.e. confounders). Results: In this work, we introduce a novel multi-view clustering method based on NMTF and NTD, named INMTD, which integrates omics and 3D imaging data to derive unconfounded subgroups of individuals. According to the adjusted Rand index, INMTD outperformed other clustering methods on a …

Nicotine Promotes Angii-Induced Abdominal Aortic Aortopathies In Female And Male Mice, Mark Ensor, Sean E. Thatcher, Kristen Mcquerry, Kory Heier, Heba M. Ali, Victoria English, Lisa A. Cassis, Yasir Alsiraj

Biostatistics Faculty Publications

Cigarette smoking is a risk factor for abdominal aortic aneurysms (AAAs), with studies suggesting a higher smoking-related AAA risk in women than men. We examined nicotine’s effects on angiotensin II (AngII)-induced AAAs in male and female low-density lipoprotein receptor-deficient (Ldlr^-/-) mice. Moreover, we defined effects of gonadectomy (GDX) of both sexes on nicotine-induced regulation of AAAs. Male and female Ldlr^-/- mice (8–12 weeks of age) were infused with AngII with or without nicotine. Mice underwent sham or GDX surgeries prior to infusions of AngII and nicotine. In males, one or both testes were removed. AAA incidence, size, …

Novel Approaches To Analyze Consumer Behavior And Policies To Promote Sustainable Consumption, Bhagyashree Katare, Xuejian Wang, Shuoli Zhao, Jacqueline Yenerall

Agricultural Economics Faculty Publications

Technological advancements, such as online grocery shopping, have significantly transformed consumer retail environments and experiences. Effectively studying consumer behavior in these new environments requires the use of novel methodological approaches, which will also aid in developing interventions to encourage sustainable consumption. This paper begins by providing an overview of the current literature on novel approaches to analyzing consumer behavior, then provides an empirical application by examining consumer decision-making pathways within an online grocery shopping platform. Specifically, the paper focuses on exploring the consumers’ digital footprints, such as page visits, product additions and removals, and interactions with information labels to identify …

The Acth Test Fails To Diagnose Adrenal Insufficiency And Augments Cytokine Production In Sepsis, Dan Hao, Qian Wang, Misa Ito, Jianyao Xue, Ling Guo, Bin Huang, Chieko Mineo, Philip W. Shaul, Xiang An Li

Physiology Faculty Publications

The adrenocorticotropic hormone (ACTH) test diagnoses relative adrenal insufficiency (RAI) or critical illness–related corticosteroid insufficiency (CIRCI). Initially, guidelines recommended corticosteroid/glucocorticoid (GC) therapy for septic patients with RAI, but later trials did not show a survival benefit, leading to updated guidelines that abandon targeting RAI or CIRCI. Recent studies with an RAI mouse model showed a clear survival benefit from GC therapy in mice with RAI, suggesting that inconclusive GC clinical trials might be due to issues with the ACTH test rather than targeting RAI. To investigate, we performed the ACTH test in septic mice. Interestingly, the ACTH test identified most …

Steroid Receptors And Coregulators: Dissemination Of Sex Differences And Emerging Technologies, Sally Pauss, Evelyn A. Bates, Genesee J. Martinez, Zane T. Bates, Zachary A. Kipp, Cassandra D. Gipson, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

Steroid receptors are ligand-induced transcription factors that have broad functions among all living animal species, ranging from control of sex differences, body weight, stress responses, and many others. Their binding to coregulator proteins is regulated by corepressors and coactivators that interchange upon stimulation with a ligand. Coregulator proteins are an imperative and understudied aspect of steroid receptor signaling. Here, we discuss steroid receptor basics from protein domain structures that allow them to interact with coregulators and other proteins, their essential functions as transcription factors, and other elemental protein–protein interactions. We deliberate about the mechanisms that coregulators control in steroid receptor …

Lysyl Hydroxylase 2 Glucosylates Collagen Vi To Drive Lung Cancer Progression, Shike Wang, Houfu Guo, Reo Fukushima, Masahiko Terajima, Min Liu, Guan-Yu Xiao, Lenka Koudelková, Chao Wu, Xin Liu, Jiang Yu, Emma Burris, Jun Xu, Alvise Schiavinato, William K. Russell, Mitsuo Yamauchi, Xiaochao Tan

Markey Cancer Center Faculty Publications

Lysyl hydroxylase 2 (LH2) is highly expressed in multiple tumor types and accelerates disease progression by hydroxylating lysine residues on fibrillar collagen telopeptides to generate stable collagen cross links in tumor stroma. Here, we show that a galactosylhydroxylysyl glucosyltransferase (GGT) domain on LH2 modified type-VI collagen (Col6) to promote lung adenocarcinoma (LUAD) growth and metastasis. In tumors generated by LUAD cells lacking LH2 GGT domain activity, stroma was less stiff, and stable types of collagen cross links were reduced. Mass spectrometric analysis of total and glycosylated peptides in parental and GGT-inactive tumor samples identified Col6 chain α3 (Col6a3), a component …

Management Of Cardiovascular Implantable Electronic Device Infection Utilizing A Multidisciplinary Team, Evan Hall, Bennett Collis, Talal Alnabelsi, Chloe Cao, Meredith Johnson, John Gurley, Luke Strnad, Hassan Reda, Tessa London, Erinn Ogburn, Michael Sekela, Bobbi Jo Stoner, Sami El-Dalati

Surgery Faculty Publications

Background. Significant numbers of cardiac implantable electronic devices are placed in the United States, and infection can be a severe consequence of implantation. Despite guideline recommendations and data demonstrating that lead extraction improves mortality for patients with device infection, rates of lead removal are lower than guideline recommendations. We report the outcomes associated with management of suspected cardiac implantable electronic device infections by a multidisciplinary team. Methods. Patient cases were identified from an institutional multidisciplinary endocarditis team registry in a single-center retrospective study. Demographic, treatment, and outcomes data were recorded by study investigators. Results. Between September 7, 2021, and February …

The Role Of Race/Ethnicity On The Association Between Neighborhood Deprivation And Breast Cancer Outcomes Among Kentucky Patients With Breast Cancer (2010–2022), Breyanna Walker, Elinita Pollard, Sydney P. Howard, V. Morgan Jones, Kathleen L. O’Connor, Eric B. Durbin, Pamela C. Hull, Samantha R. Jones, Adebola Adegboyega, Xiaoqin Wang, Wendi A.B. Owen, Margaret M. Szabunio, Lovoria B. Williams, Justin X. Moore

Surgery Faculty Publications

Background: Kentucky is within the top five leading states for breast cancer mortality nationwide. This study investigates the association between neighborhood socioeconomic disadvantage and breast cancer outcomes, including surgical treatment, radiotherapy, chemotherapy, and survival, and how associations vary by race and ethnicity in Kentucky. Methods: We conducted a retrospective cohort analysis using data from the Kentucky Cancer Registry for patients with breast cancer diagnosed between 2010 and 2017, with follow-up through December 31, 2022. We linked Kentucky Cancer Registry data with census tract data to examine the relationship between area deprivation index (ADI) and breast cancer outcomes. Logistic regression and …

Characterization Of Tnsalp Expression And Activity In Porcine Utero-Placental Tissues, Claire Stenhouse, Nirvay Sah, Katherine M. Halloran, Robyn M. Moses, Makenzie G. Newton, Joe W. Cain, Heewon Seo, Guoyao Wu, Gregory A. Johnson, Fuller W. Bazer

Animal and Food Sciences Faculty Publications

Abstract: Tissue non-specific alkaline phosphatase (TNSALP) regulates postnatal phosphate homeostasis, but its role in utero-placental phosphate availability remains poorly understood. Gilts were bred and hysterectomized on Day 60 or Day 90 of gestation (n = 6/day). Phosphate was less abundant in allantoic and amniotic fluids on Day 90 compared to Day 60. TNSALP protein was immunolocalized, and enzymatic activity was quantified and localized in endometrial and chorioallantois tissues. Day had no effect on TNSALP activity in the chorioallantois. In contrast, endometrial TNSALP activity was lower on Day 90 compared to Day 60. Phosphate abundance in allantoic fluid correlated positively with …

Separation And Quantification Of Mycotoxins In Floral Tissue Of Hemp (Cannabis Sativa) Infected With Fusarium Graminearum, Isabelle A. Kagan, Henry S. Smith, Rachel R. Schendel, Nicole Gauthier

Animal and Food Sciences Faculty Publications

Background: The fungal pathogen Fusarium graminearum produces mycotoxins when it infects grains. This pathogen also infects hemp (Cannabis sativa L. containing less than 0.3% tetrahydrocannabinol or THC). The presence of trichothecene mycotoxins like deoxynivalenol (DON) has not been reported in floral tissue of hemp infected with F. graminearum. The object of this study was to develop a method to extract three trichothecene mycotoxins—DON, 3-acetyldeoxynivalenol (3-ADON), and 15-acetyldeoxynivalenol (15-ADON)—from hemp floral tissue, and to quantify the mycotoxins by high-performance liquid chromatography (HPLC) with ultraviolet detection. Results: Standards of nivalenol (NIV), DON-3-glucoside (D3G), DON, 3-ADON, and 15-ADON were separated from each other …

Structural Assembly Of The Pas Domain Drives The Catalytic Activation Of Metazoan Pask, Sajina Dhungel, Michael Xiao, Rajesh Rajaian Pushpabai, Chintan K. Kikani

Biology Faculty Publications

PAS domains are ubiquitous sensory modules that transduce environmental signals into cellular responses through tandem PAS folds and PAS-associated C-terminal (PAC) motifs. While this conserved architecture underpins their regulatory roles, here we uncover a structural divergence in the metazoan PAS domain-regulated kinase (PASK). By integrating evolutionary-scale domain mapping with deep learning-based structural models, we identified two PAS domains in PASK, namely PAS-B and PAS-C, in addition to the previously known PAS-A domain. Unlike canonical PAS domains, the PAS fold and PAC motif in the PAS-C domain are spatially segregated by an unstructured linker, yet a functional PAS module is assembled …

Assessing Coronavirus Disease 2019 Pandemic Impacts On The Health Of People Who Inject Drugs Using A Novel Data Sharing Model, Heather Bradley, Nicole Luisi, Anastasia Carter, Terri D. Pigott, Daniela Abramovitz, Sean T. Allen, Alice Asher, Chelsea Austin, Tyler S. Bartholomew, Marianna Baum, Amy Board, Basmattee Boodram, Annick Borquez, Kathryn A. Brookmeyer, Kate Buchacz, Janet Burnett, Hannah L.F. Cooper, Nicole Crepaz, Kora Debeck, Judith Feinberg, Chunki Fong, Edward Freeman, Nathan Woo Furukawa, Becky Genberg, Pamina Gorbach, Holly Hagan, Kanna Hayashi, Emalie Huriaux

Epidemiology and Environmental Health Faculty Publications

Objective: Using an innovative data sharing model, we assessed the impacts of the COVID-19 pandemic on the health of people who inject drugs (PWID). Design: The PWID Data Collaborative was established in 2021 to promote data sharing across PWID studies in North America. Contributing studies submitted aggregate data on 23 standardized indicators during four time periods: prepandemic (March 2019 to February 2020), early-pandemic (March 2020 to February 2021), mid-pandemic (March 2021 to February 2022), and late pandemic (March 2022 to February 2023). Methods: We present study-specific and meta-analyzed estimates for the percentage of PWID who took medications for opioid use …

A Satellite Cell-Dependent Epigenetic Fingerprint In Skeletal Muscle Identity Genes After Lifelong Physical Activity, Kevin A. Murach, Davis A. Englund, Toby L. Chambers, Cory M. Dungan, Hunter L. Porter, Jonathan D. Wren, Willard M. Freeman, Esther E. Dupont-Versteegden, Yuan Wen

Physiology Faculty Publications

Satellite cells comprise a small proportion of mononuclear cells in adult skeletal muscle. Despite their relative rarity, satellite cells have critical functions in muscle adaptation, particularly during prolonged exercise training. The mechanisms by which satellite cells mediate skeletal muscle responsiveness to physical activity throughout the lifespan are still being defined, but epigenetic regulation may play a role. To explore this possibility, we analyzed global DNA methylation patterns in muscle tissue from female mice that engaged in lifelong voluntary unweighted wheel running with or without satellite cells. Satellite cells were ablated in adulthood using the tamoxifen-inducible Pax7-DTA model. Compared to sedentary …

Factors Influencing The Availability Of Soil Cobalt And Its Uptake By Herbage, J E. Paterson, D A. Klessa, A Macpherson

IGC Proceedings (1977-2023)

Severe cobalt (Co) deficiency is now rare in Scotland but subclinical deficiency, although difficult to diagnose, is com­monly thought to be reducing the profitability of many sheep and beef farms (COSAC/SARI, 1982). By understanding the factors which determine herbage Co content and Co availablity to ruminants, areas at risk may be predicted and preventative measures taken. To this end, a series of field and laboratory experiments were investigated:

Co adsorption and desorption characteristic of soils. Effects of fertiliser N on herbage Co content. Response of pasture and grazing ruminants to applied Co fertiliser.

Could Psilocin Rescue Chronic, Stress-Accelerated, Transcriptional Aging In Brain?, Danny R. Craig, Eric M. Blalock

Journal of Pharmacology & Nutritional Sciences

Brain aging (BA) processes are complex, often affect multiple systems, and frequently lead to cognitive decline and increased susceptibility to insults. BA appears to be a primary risk for the development of many prominent neurodegenerative pathologies. The US Census Bureau predicts that the aging population (65+) will represent a greater proportion of the US population than children (under 18) by 2034, dramatically increasing the burden on health-care infrastructure.¹⁷ Several mechanisms of stress driven, aging-related neurologic dysfunction have been advanced in the past 50 years and include: Stress hormone exposure and glucocorticoid cascade;^{7, 16} calcium ion dyshomeostasis;⁸ allostatic …

Abuse-Deterrent Formulations And Opioid-Related Harms In North Carolina, 2010-2018, Bethany L. Diprete, Nabarun Dasgupta, G. Yeon Oh, Daniela C. Moga, Svetla Slavova, Emily Slade, Chris Delcher, Brian W. Pence, Shabbar I. Ranapurwala

Epidemiology and Environmental Health Faculty Publications

Abuse-deterrent formulations of opioid analgesics (ADFs) were introduced to reduce opioid-related harms among pain patients, but postmarketing study results have been mixed. However, these studies may be subject to bias from selection criteria, comparator choice, and potential confounding by “indication,” highlighting the need for thorough study design considerations. In a sample of privately insured patients prescribed ADF or non-ADF extended-release/long-acting (ER/LA) opioids in North Carolina, we implemented a version of the prevalent new-user design to evaluate the relationship between ADFs and opioid use disorder (OUD, n = 235) and opioid overdose (n = 18) through 6 months of follow-up using …

Quantitative And Narrative Analysis Of Dump Truck-Related Injuries And Fatalities In The United States, Terry L. Bunn, Caitlin A. Northcutt, Rebecca Honaker, Patrick Maloney

Epidemiology and Environmental Health Faculty Publications

Dump truck safety recommendations have been implemented by companies, but a comprehensive study of risk factors associated with dump truck injuries and fatalities has not been performed. Published research specifically focusing on dump truck injuries is limited. The purpose of this descriptive study was to characterize risk factors associated with dump truck-related inspection cases reported by the OSHA in the United States. Dump truck inspections during 2016–2020 were obtained from the online OSHA IMIS database. Pearson’s chi-square test and logistic regression were performed. An intraclass correlation coefficient (ICC) using PROC MIXED and a generalized linear mixed model was calculated on …

Among-Population Variation In Telomere Regulatory Proteins And Their Potential Role As Hidden Drivers Of Intraspecific Variation In Life History, Sarah E. Wolf, Mary J. Woodruff, David A. Chang Van Oordt, Ethan D. Clotfelter, Daniel A. Cristol, Elizabeth P. Derryberry, Stephen M. Ferguson, Mark T. Stanback, Conor C. Taff, Maren N. Vitousek, David F. Westneat, Kimberly A. Rosvall

Biology Faculty Publications

Biologists aim to explain patterns of growth, reproduction and ageing that characterize life histories, yet we are just beginning to understand the proximate mechanisms that generate this diversity. Existing research in this area has focused on telomeres but has generally overlooked the telomere's most direct mediator, the shelterin protein complex. Shelterin proteins physically interact with the telomere to shape its shortening and repair. They also regulate metabolism and immune function, suggesting a potential role in life history variation in the wild. However, research on shelterin proteins is uncommon outside of biomolecular work. Intraspecific analyses can play an important role in …

Scaling Covid-19 Rates With Population Size In The United States, Austin R. Cruz, Brian J. Enquist, Joseph R. Burger

Biology Faculty Publications

Using county-level data from the United States, we assessed allometric scaling relationships of coronavirus disease (COVID-19) cases, deaths and age structure within and across the first four major waves of the pandemic (wild-type, alpha, delta, omicron). Results generally indicate that the burden of cases disproportionately impacted larger-sized counties, while the burden of deaths disproportionately impacted smaller counties. This may be partially due to multiple interacting social mechanisms, including a higher proportion of older adults who live in smaller counties. Moreover, these likely social mechanisms interacting with vaccinations and virus waves created a dynamic pattern whereby the rate and magnitude of …

Data Reporting Quality And Semantic Interoperability Increase With Community-Based Data Elements (Codes). Analysis Of The Open Data Commons For Spinal Cord Injury (Odc-Sci), Anushka Sheoran, Kenneth A. Fond, Lex Maliga Davis, J. R. Huie, Romana Vavrek, P. J. Axtman, Vance P. Lemmon, John L. Bixby, Ubbo Visser, John C. Gensel, Karim Fouad, Adam R. Ferguson, Jeffrey S. Grethe, Anita Bandrowski, Maryann E. Martone, Abel Torres-Espin

Neurology Faculty Publications

Data interoperability is crucial for effectively combining data for scientific inquiry. To facilitate interoperability, data standards such as a common definition of variables are often developed. The Open Data Commons for Spinal Cord Injury (odc-sci.org) has established an initial set of community-based data elements (CoDEs)—a minimal set of variables for sharing—to promote data interoperability in SCI research, aligning with FAIR (Findable, Accessible, Interoperable, and Reusable) data principles. We sought to understand the use of CoDEs by the SCI community to inform current standards adherence and future standards development. We systematically analyzed 39 public datasets in relation to 17 required CoDEs …

Sociodemographic Factors Associated With Prenatal Care Utilization In Arkansas, United States., Pearl A Mcelfish, Aaron Caldwell, Donya Watson, Jonathan Langner, Jennifer Callaghan-Koru, Austin Porter, Don E Willis, Jennifer A Andersen, Nicola L Hawley, James P Selig, Amir Forati, Maria R Alcala, Lanita White, Enrique Gomez-Pomar, Clare C Brown

Pediatrics Faculty Publications

OBJECTIVES: This study examines prenatal care utilization differences in Arkansas.

METHODS: Birth records data from the National Center for Health Statistics were used. The study population consisted of singleton live births in Arkansas from 2014 to 2022. Primary outcomes included number of prenatal visits, fewer than the recommended number of prenatal visits, late prenatal care, and no prenatal care. Summary statistics were computed, and adjusted rates ratios were calculated adjusting for maternal age, maternal education, and parity for payer, race/ethnicity, and rural/urban residence.

RESULTS: Mothers with a Medicaid-covered birth had 0.90 times fewer prenatal care visits and were 1.26 times …