Digital Commons Network^™

Nanoparticle Brain Delivery: A Guide To Verification Methods, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

Many reports conclude nanoparticle (NP) brain entry based on bulk brain analysis. Bulk brain includes blood, cerebrospinal fluid and blood vessels within the brain contributing to the blood-brain and blood-cerebrospinal fluid barriers. Considering the brain as neurons, glia and their extracellular space (brain parenchyma), most studies did not show brain parenchymal NP entry. Blood-brain and blood-cerebrospinal fluid barriers anatomy and function are reviewed. Methods demonstrating brain parenchymal NP entry are presented. Results demonstrating bulk brain versus brain parenchymal entry are classified. Studies are reviewed, critiqued and classified to illustrate results demonstrating bulk brain versus parenchymal entry. Brain, blood and peripheral …

Itga2 Promotes Expression Of Acly And Ccnd1 In Enhancing Breast Cancer Stemness And Metastasis, Valery Adorno-Cruz, Andrew D. Hoffmann, Xia Liu, Nurmaa K. Dashzeveg, Rokana Taftaf, Brian Wray, Ruth A. Keri, Huiping Liu

Toxicology and Cancer Biology Faculty Publications

Cancer metastasis is largely incurable and accounts for 90% of breast cancer deaths, especially for the aggressive basal-like or triple negative breast cancer (TNBC). Combining patient database analyses and functional studies, we examined the association of integrin family members with clinical outcomes as well as their connection with previously identified microRNA regulators of metastasis, such as miR-206 that inhibits stemness and metastasis of TNBC. Here we report that the integrin receptor CD49b-encoding ITGA2, a direct target of miR-206, promotes breast cancer stemness and metastasis. ITGA2 knockdown suppressed self-renewal related mammosphere formation and pluripotency marker expression, inhibited cell cycling, compromised …

Impaired Meningeal Lymphatic Vessel Development Worsens Stroke Outcome, Pavel Yanev, Katherine Poinsatte, Devon Hominick, Noor Khurana, Kielen R. Zuurbier, Marcus Berndt, Erik J. Plautz, Michael T. Dellinger, Ann M. Stowe

Neurology Faculty Publications

The discovery of meningeal lymphatic vessels (LVs) has sparked interest in identifying their role in diseases of the central nervous system. Similar to peripheral LVs, meningeal LVs depend on vascular endothelial growth factor receptor-3 (VEGFR3) signaling for development. Here we characterize the effect of stroke on meningeal LVs, and the impact of meningeal lymphatic hypoplasia on post-stroke outcomes. We show that photothrombosis (PT), but not transient middle cerebral artery occlusion (tMCAo), induces meningeal lymphangiogenesis in young male C57Bl/J6 mice. We also show that Vegfr3^wt/mut mice develop significantly fewer meningeal LVs than Vegfr3^wt/wt mice. Again, meningeal lymphangiogenesis occurs in …

A Population-Based Investigation Of Health-Care Needs And Preferences In American Adults With Multiple Sclerosis, Chungyi Chiu, Malachy Bishop, Bradley Mcdaniels, Byung-Jin Kim, Lebogang Tiro

Early Childhood, Special Education, and Counselor Education Faculty Publications

Background: Comprehensive and effective multiple sclerosis (MS) health care requires understanding of patients’ needs, preferences, and priorities. Objective: To evaluate priorities of patients with MS for their MS care. Methods: Participants included 3003 Americans with MS recruited through the National MS Society and the North American Research Committee on Multiple Sclerosis patient registry. Participants completed a comprehensive questionnaire on aspects of their health-care experiences. Results: Participants identified the top 3 health-care priorities as (1) the affordability of MS health care, (2) ensuring that non-MS health-care providers have more education about MS and how it can interact with other conditions, and …

Lung Metastasis Postradioembolization Of Hepatocellular Carcinoma With Tumor In Vein, Harit Kapoor, Sreeja Sanampudi, Joseph W. Owen, Driss Raissi

Radiology Faculty Publications

Transarterial radioembolization (TARE) is one of the few treatment options available for infiltrative hepatocellular carcinoma with tumor in vein. This is backed by the published data showing marginally favorable toxicity profile compared with other locoregional and systemic therapies. Although lung shunt fraction studies are performed to prevent radiation injury to the lungs, TARE-induced embolization/metastasis to the lungs has not been reported before. We report an intriguing case of new lung metastases within 1 month after TARE for infiltrative hepatocellular carcinoma with a tumor in the vein, with only a slightly elevated but acceptable lung shunt fraction. This report brings to …

Effects Of Stocking Rate On The Variability Of Ecosystem Productivity In Desert Steppe, Zhongwu Wang, Shuying Jiao, Guodong Han, Mengli Zhao, Jing Wang, Walter D. Willms

IGC Proceedings (1977-2023)

Management practices can increase biodiversity and generate corresponding compensatory effects on biomass production, which may reduce inter-annual variability of productivity in some grassland ecosystems. However, it remains unclear how stocking rate influences variability of ecosystem productivity. Four stocking rates were compared in a completely randomized block experiment in the desert steppe of Inner Mongolia, China: non-grazed control (0 sheep/ha/mo), lightly grazed (LG, 0.15 sheep/ha/mo), moderately grazed (MG, 0.30 sheep/ha/mo) and heavily grazed (HG, 0.45 sheep/ha/mo). Aboveground net primary productivity (ANPP) was measured every August for eight consecutive years (2004-2011). ANPP decreased significantly (P < 0.05) with increasing stocking rate. Coefficients of variation for community (CV_comm) in LG and MG …

Protein Tyrosine Phosphatase 4a3 (Ptp4a3/Prl-3) Drives Migration And Progression Of T-Cell Acute Lymphoblastic Leukemia In Vitro And In Vivo, Min Wei, Meghan G. Haney, Dylan R. Rivas, Jessica S. Blackburn

Molecular and Cellular Biochemistry Faculty Publications

T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive blood cancer. There are no immunotherapies and few molecularly targeted therapeutics available for treatment of this malignancy. The identification and characterization of genes and pathways that drive T-ALL progression are critical for the development of new therapies for T-ALL. Here, we determined that the protein tyrosine phosphatase 4A3 (PTP4A3 or PRL-3) plays a critical role in T-ALL initiation and progression by promoting leukemia cell migration. PRL-3 is highly expressed in patient T-ALL samples at both the mRNA and protein levels compared to normal lymphocytes. Knock-down of PRL-3 expression using short-hairpin RNA (shRNA) …

Short-Chain Fatty Acids Improve Poststroke Recovery Via Immunological Mechanisms, Rebecca Sadler, Julia V. Cramer, Steffanie Heindl, Sarantos Kostidis, Dene Betz, Kielen R. Zuurbier, Bernd H. Northoff, Marieke Heijink, Mark P. Goldberg, Erik J. Plautz, Stefan Roth, Rainer Malik, Martin Dichgans, Lesca M. Holdt, Corinne Benakis, Martin Giera, Ann M. Stowe, Arthur Liesz

Neurology Faculty Publications

Recovery after stroke is a multicellular process encompassing neurons, resident immune cells, and brain-invading cells. Stroke alters the gut microbiome, which in turn has considerable impact on stroke outcome. However, the mechanisms underlying gut–brain interaction and implications for long-term recovery are largely elusive. Here, we tested the hypothesis that short-chain fatty acids (SCFAs), key bioactive microbial metabolites, are the missing link along the gut–brain axis and might be able to modulate recovery after experimental stroke. SCFA supplementation in the drinking water of male mice significantly improved recovery of affected limb motor function. Using in vivo wide-field calcium imaging, we observed …

Psychosocial Mediators Of Perceived Stigma And Suicidal Ideation Among Transgender Women, Krishna Kiran Kota, Laura F. Salazar, Rachel E. Culbreth, Richard A. Crosby, Jamal Jones

Health, Behavior & Society Faculty Publications

BACKGROUND: Transgender women (TGW) in the U.S. experience high rates of stigma, depression, and elevated rates of suicide. This study examined correlates of suicidal ideation and estimated the conditional indirect effects of perceived stigma and psychosocial mediators on suicidal ideation.

METHODS: Using a cross-sectional study design, TGW (N = 92) were recruited through snowball sampling in Atlanta, Georgia. Structured interviews were conducted. Suicidal ideation was assessed by combining two variables that measured suicidal thoughts. Logistic regression models were performed to identify the potential risk and protective factors for suicidal ideation. We examined hypothesized psychosocial factors, including anxiety, depression, psychosocial impact …

Identification Of Ebsulfur Analogues With Broad-Spectrum Antifungal Activity, Sylvie Garneau-Tsodikova, Huy X. Ngo, Sanjib K. Shrestha

Pharmaceutical Sciences Faculty Patents

The invention relates to novel ebsulfur analogues and novel pharmaceutical compositions comprising ebsulfur analogues. The invention also relates to novel methods of treating infections caused by fungal species comprising administration of ebselen, ebsulfur, and ebsulfur analogues.

A Brief Educational Intervention Enhances Basic Cancer Literacy Among Kentucky Middle And High School Students, Lauren Hudson, Kerrigan M. Samons, Haley E. Dicken, Chris Prichard, L. Todd Weiss, Jean Edward, Robin C. Vanderpool, Nathan L. Vanderford

Markey Cancer Center Faculty Publications

Kentucky experiences the highest overall cancer incidence and mortality rates in the USA with the greatest burden in the eastern, Appalachian region of the state. Cancer disparities in Kentucky are driven in part by poor health behaviors, poverty, lack of health care access, low education levels, and low health literacy. Individuals with inadequate health literacy are less likely to participate in preventive measures such as obtaining screenings and making healthy lifestyle choices, thus increasing their chances of developing and dying from cancer. By increasing cancer literacy among youth and adults, it may be possible to decrease cancer disparities across Kentucky. …

Reviewer Acknowledgments For 2019, F. Douglas Scutchfield Md, Erin N. Haynes, Erin Bouldin, Robert M. Shapiro Ii, Tim Marema, Charlotte S. Seidman

Journal of Appalachian Health

The Editorial Team extends a heart-felt “thank you” to those who have given their time and expertise in the past year to participate in this process with the Journal of Appalachian Health. We know that you have many competing pressures on your time, and that you are not financially compensated for the time you spend reviewing manuscripts. We hope that there are other forms of compensation that make the sacrifice worth the effort.

A Healthy Attitude: Rural Leaders In Tn County Organize To Address Well-Being In Appalachia, Tim Marema, Erin Bouldin

Journal of Appalachian Health

When it came to formal philanthropy, Grundy County was not on the map. That changed with the 2012 establishment of South Cumberland Community Fund, which serves the plateau portions of Grundy, Franklin, and Marion counties.

The Nuclear Legacy In Appalachia, Michele Morrone, Harold Perkins

Journal of Appalachian Health

Nestled in the rolling hills of Appalachia Ohio is a reminder of the role that the region played in winning the Cold War. For more than 40 years in rural Pike County, the 3,700-acre Portsmouth Gaseous Diffusion Plant (PORTS), or the “A-Plant” as the locals refer to it, enriched uranium for use in nuclear weapons. While the facility produced nuclear fuel for national security, it simultaneously exposed plant workers to chemicals and radiation and discharged pollution into the surrounding community. The A-Plant is now being demolished and the site repurposed. However, the site continues to affect the community as, for …

Appalachian Environmental Health Literacy: Building Knowledge And Skills To Protect Health, Anna G. Hoover, Annie Koempel, W. Jay Christian, Kimberly I. Tumlin, Kelly G. Pennell, Steven Evans, Malissa Mcalister, Lindell E. Ormsbee, Dawn Brewer

Journal of Appalachian Health

Environmental health literacy (EHL) is an emerging, multidisciplinary field that promotes understanding of how environmental exposures can affect human health. After discussing the regional relevance of environmental health knowledge and skills, this article describes three ongoing Appalachian projects that are focused on measuring and building EHL.

Development And Early Outcomes Of The Watauga Compassionate Community Initiative, North Carolina, Tim Marema, Erin Bouldin

Journal of Appalachian Health

Addressing adverse childhood experiences has become a public health imperative, and communities across the United States are working to develop and implement programs and policies to both prevent childhood trauma and support adults who experienced trauma as children. Here we describe the development of the Watauga Compassionate Community Initiative (WCCI) in Watauga, County, North Carolina.

Access To Autism Spectrum Disorder Services For Rural Appalachian Citizens, Angela Scarpa, Laura S. Jensen, Denis Gracanin, Sharon L. Ramey, Angela V. Dahiya, L. Maria Ingram, Jordan Albright, Alyssa J. Gatto, Jen P. Scott, Lisa Ruble

Journal of Appalachian Health

Background: Low-resource rural communities face significant challenges regarding availability and adequacy of evidence-based services.

Purposes: With respect to accessing evidence-based services for Autism Spectrum Disorder (ASD), this brief report summarizes needs of rural citizens in the South-Central Appalachian region, an area notable for persistent health disparities.

Methods: A mixed-methods approach was used to collect quantitative and qualitative data during focus groups with 33 service providers and 15 caregivers of children with ASD in rural southwest Virginia.

Results: Results supported the barriers of availability and affordability of ASD services in this region, especially relating to the need for more ASD-trained providers, …

Introduction To The New “Early Reports Of Innovation” Section, Erin Bouldin, Tim Marema

Journal of Appalachian Health

The Journal of Appalachian Health is introducing a new section this issue. While the journal is centralizing some of the best research and commentary on Appalachian health, the editorial team felt that practice-focused groups, organizations, and agencies may not be fully represented in the publication.

Barn-Raising On The Digital Frontier: The L.A.U.N.C.H. Collaborative, Bradford W. Hesse, David Ahern, Michele Ellison, Eliah Aronoff-Spencer, Robin C. Vanderpool, Karen Onyeije, Michael C. Gibbons, Timothy W. Mullett, Ming-Yuan Chih, Victoria Attencio, Grant Patterson, Jessica Boten, Christopher Hartshorn, Ben Bartolome, Katie Gorscak, Melanie Mccomsey, Alexandra Hubenko, Bin Huang, Corey Baker, Don Norman

Journal of Appalachian Health

A meta-analysis of oncology papers from around the world revealed that cancer patients who lived more than 50 miles away from hospital centers routinely presented with more advanced stages of disease at diagnosis, exhibited lower adherence to prescribed treatments, presented with poorer diagnoses, and reported a lower quality of life than patients who lived nearer to care facilities. Connected health approaches—or the use of broadband and telecommunications technologies to evaluate, diagnose, and monitor patients beyond the clinic—are becoming an indispensable tool in medicine to overcome the obstacle of distance.

Introducing The L.A.U.N.C.H. Collaborative, F. Douglas Scutchfield Md, Kevin Patrick

Journal of Appalachian Health

The L.A.U.N.C.H. Collaborative: Linking & Amplifying User-Centered Networks through Connected Health: A Demonstration of Broadband-Enabled Connected Health and Community-Based Co-Design brings together a group of organizations that are eager to use Appalachian Kentucky as a site for the development of a project aimed at creating an environment that addresses two of the nation’s major concerns about cancer. First, individuals who live in rural and remote areas are more likely to die of cancer than those who live in urban or suburban settings. And second, geographic obstacles hinder their ability to access evidence-based strategies that can prevent cancer or treat it …

Students’ Perceptions Of Justice: Application Of Sanctions, Guilt, And Responsibility In Campus Sexual Assault Cases, Diane R. Follingstad, Caihong R. Li, Jaspreet K. Chahal, Claire M. Renzetti

CRVAW Faculty Journal Articles

Despite growing attention to adjudication of campus sexual assault cases, little is known how students perceive “justice” for such cases. The present study examined whether victim, perpetrator, and assault characteristics influenced students’ perceptions of: whether a sanctionable violation occurred; the type of sanction to be applied; perceived severity of the sanction; proportion of guilt attributable to the victim and perpetrator; and level of responsibility of the victim and perpetrator. Fourteen factors pertaining to potential negative evaluation of rape victims were derived; thus, a non-factorial vignette survey design focusing only on each main effect was employed. 846 college students responded to …

Identifying Grazing-Driven Plant Indicators Of Rangeland Degradation In Semi Arid Zones Of Uzbekistan, Toshpulot F. Rajabov, Bakhtiyor K. Mardonov, Mahmud A. Muminov

IGC Proceedings (1977-2023)

The development of practical indicators of vegetation degradation is an important element in predicting and assessing rangeland health due to disturbances such as livestock grazing. Once established, phytoindicators provide a simple and visual operative way to diagnose active and possibly undesirable vegetation changes. This paper aimed to identify rapid, yet realistic and easily communicated plant indicators of rangeland degradation caused by intense grazing. The grazing gradient approach is applied to study plant traits affected by increased levels of grazing pressure. Qualitative and quantitative changes in key species of the plant community alongside successive changes of vegetation attributes along an apparent …

Mentoring Multi-College Bystander Efficacy Evaluation – An Approach To Growing The Next Generation Of Gender-Based Interpersonal Violence Intervention And Prevention (Vip) Researchers, Ann L. Coker, Danielle Davidov, Heather M. Bush, Emily R. Clear

Obstetrics and Gynecology Faculty Publications

The Centers for Disease Control and Prevention provided funding (U01 CE002668) to evaluate bystander program efficacy to reduce gender-based violence on college campuses (Aim 1) and to create a mentoring network (Aim 2) for young campus-based researchers interested in violence intervention or prevention (VIP). While an evaluation of this mentoring program is ongoing, our purpose here was to document the strategies used to create, implement, and begin evaluation of this national multi-college mentoring network. As each public college was recruited into this evaluation named multi-college Bystander Efficacy Evaluation (mcBEE), each college was invited to nominate a researcher interested in receiving …

Monitoring Landscape Changes Using Remote Sensing Technology In Southern Africa, Anthony R. Palmer

IGC Proceedings (1977-2023)

Rangelands are a major global resource, and there is an urgency to improve the assessment of landscape performance to capture carbon, produce biomass, and improve water use. Effective monitoring necessitates the collection of high quality rangeland condition data using repeatable techniques. Despite much effort, there are few comprehensive data sets that allow confident detection of landscape change. Data are lacking for several reasons, including high cost of data collection, conflicting methodologies, and loss of archival data. Satellite imagery provides the basis for trend and pattern analysis of rangeland in different conditions classes and this paper presents some examples of the …

The Nutritional Status Of Japanese Black Breeding Cows Grazed In A Larger Abandoned Paddy Field, Motohiko Ishida, Naoki Matsumoto, Yojiro Maki, Jyunichi Tanimoto, Kohei Takahashi, Keigo Asano, Mizuho Ogino

IGC Proceedings (1977-2023)

Because rice consumption has decreased in Japan, the number of abandoned paddy fields has increased. Some of the abandoned paddy fields are being used for livestock grazing, effectively using the abandoned fields and reducing the production costs on livestock farms. Such grazing may use an integrated grazing system of scattered small pastures where each of the small abandoned paddy fields scattered in a given agriculture area is defined as one grazing yard, and a herd consisting of two or three head of cattle is moved from one grazing yard to another. However, because the number of abandoned paddy fields has …

Grazing Termination Dates Of Summer-Dormant Flecha Tall Fescue, Sindy M. Interrante, Twain J. Butler

IGC Proceedings (1977-2023)

Summer-dormant, Mediterranean-type tall fescue (Festuca arundiancea Schreb.) has potential to replace summer-active, Continental-type tall fescue and trad-itional, annual small grain graze-out systems in the Southern Great Plains region of the USA. This region is characterized by severe water deficits accompanied by extreme heat in summer, and by relatively mild, rainy winters (Malinowski et al. 2009). Although the climate of the southern Great Plains is different from the Mediterranean climate, the temperature and precipitation patterns during summer are historically similar. However, in the past decade, the bimodal precipitation pattern with peaks in May and September has become highly unpredictable, …

Review Of Alterations In Perlecan-Associated Vascular Risk Factors In Dementia, Amanda L. Trout, Ibolya Rutkai, Ifechukwude J. Biose, Gregory J. Bix

Neurology Faculty Publications

Perlecan is a heparan sulfate proteoglycan protein in the extracellular matrix that structurally and biochemically supports the cerebrovasculature by dynamically responding to changes in cerebral blood flow. These changes in perlecan expression seem to be contradictory, ranging from neuroprotective and angiogenic to thrombotic and linked to lipid retention. This review investigates perlecan's influence on risk factors such as diabetes, hypertension, and amyloid that effect Vascular contributions to Cognitive Impairment and Dementia (VCID). VCID, a comorbidity with diverse etiology in sporadic Alzheimer's disease (AD), is thought to be a major factor that drives the overall clinical burden of dementia. Accordingly, changes …

Effect Of Enclosure On Botanical Composition, Forage Biomass And Other Range Productivity Parameters In A Semi-Arid Area Of El-Khuwei Locality, North Kordofan State, Sudan, Mohammed Abdelkreim, Babo Fadlalla

IGC Proceedings (1977-2023)

Animal production in North Kordofan State is mainly traditional depending on natural rangeland (Cook and Fadlalla 1987), (DHP, 1998). The State has an area of about 25 million ha of which 14.5 million ha is rangeland (Africover 2004). It is among the leading Sudanese States in animal and range resources. It contains more than 13 million heads of sheep, goats, camels and cattle (IFAD 2011). This paper deals with the effect of enclosure and stage of maturity on botanical composition, forage biomass production and other range productivity parameters in this semi-arid area.

Dry Season Forages For Improving Dairy Cattle Production In Smallholder Dairy Systems, Jolly Kabirizi, Emma Zziwa, Swidiq Mugerwa, Agnes Namagembe

IGC Proceedings (1977-2023)

Economically feasible strategies for year-round feed supply to dairy cattle are needed to improve feed resource availability, milk yield and household income for the smallholder dairy farming systems that dominate in the rural Eastern and Central African region. Currently, napier grass (Pennisetum purpureum) is the major forage in zero-grazing production systems, but production is often constrained. While forage legumes, including Centrosema pubescens and Clitoria ternatea are used to complement napier grass, Brachiaria spp, a new drought tolerant grass, is being introduced. Our results showed that the introduction of drought tolerant forage technologies improved milk yield and household income. …

Relative Losses Of Pioneiro Grass (Pennisetum Purpureum Schumach) Silage With Addition Of Whole Plant Maize Or Maize Grain During Fermentation, Tiago M. Santos, Eduardo R. Scherer, Dayanna Pastal, Diana R. Vivian, Kátia F. Gobbi, Américo F. Garcez Neto

IGC Proceedings (1977-2023)

Among the forages with potential to be ensiled, tropical forages stand out because of their high yield capacity, being able to produce three times more dry matter than for maize under tropical conditions. Although tropical forages are an interesting alternative for ensilage, their high moisture content may impair the fermentation process, reducing the silage quality. The addition of whole plant maize and maize grain to other grass silages can be an efficient way to reduce the losses involved during the process, specially due to the improvement of the fermentative profile of the silages (Stefanie et al. 2000; Anaya-Ortega et …