Digital Commons Network^™

Effects Of Supplementation Of Microalgae (Aurantiochytrium Sp.) To Laying Hen Diets On Fatty Acid Content, Health Lipid Indices, Oxidative Stability, And Quality Attributes Of Meat, Bing Liu, Jiang Jiang, Dongyou Yu, Gang Lin, Youling L. Xiong

Animal and Food Sciences Faculty Publications

The present study is conducted to investigate the effects of dietary docosahexaenoic acid (DHA)-rich microalgae (MA, Aurantiochytrium sp.) on health lipid indices, stability, and quality properties of meat from laying hens. A total of 450 healthy 50-wk-old Hy-Line Brown layers were randomly allotted to 5 groups (6 replicates of 15 birds each), which received diets supplemented with 0, 0.5, 1.0, 1.5, and 2.0% MA for 15 weeks. Fatty acid contents and quality properties of breast and thigh muscles from two randomly selected birds per replicate (n = 12) were measured. The oxidative stability of fresh, refrigerated, frozen, and cooked …

Simulating Phase Transitions And Control Measures For Network Epidemics Caused By Infections With Presymptomatic, Asymptomatic, And Symptomatic Stages, Benjamin Braun, Başak Taraktaş, Brian Beckage, Jane Molofsky

Mathematics Faculty Publications

We investigate phase transitions associated with three control methods for epidemics on small world networks. Motivated by the behavior of SARS-CoV-2, we construct a theoretical SIR model of a virus that exhibits presymptomatic, asymptomatic, and symptomatic stages in two possible pathways. Using agent-based simulations on small world networks, we observe phase transitions for epidemic spread related to: 1) Global social distancing with a fixed probability of adherence. 2) Individually initiated social isolation when a threshold number of contacts are infected. 3) Viral shedding rate. The primary driver of total number of infections is the viral shedding rate, with probability of …

Recovery From Covid-19 And Acute Respiratory Distress Syndrome: The Potential Role Of An Intensive Care Unit Recovery Clinic: A Case Report, Kirby P. Mayer, Jamie L. Sturgill, Anna G. Kalema, Melissa K. Soper, Sherif M. Seif, Evan P. Cassity, Jimmi Hatton Kolpek, Esther E. Dupont-Versteegden, Ashley A. Montgomery-Yates, Peter E. Morris

Physical Therapy Faculty Publications

Background

In this case report, we describe the trajectory of recovery of a young, healthy patient diagnosed with coronavirus disease 2019 who developed acute respiratory distress syndrome. The purpose of this case report is to highlight the potential role of intensive care unit recovery or follow-up clinics for patients surviving acute hospitalization for coronavirus disease 2019.

Case Presentation

Our patient was a 27-year-old Caucasian woman with a past medical history of asthma transferred from a community hospital to our medical intensive care unit for acute hypoxic respiratory failure due to bilateral pneumonia requiring mechanical ventilation (ratio of arterial oxygen partial …

Upregulation Of Cpt1a Is Essential For The Tumor-Promoting Effect Of Adipocytes In Colon Cancer, Xiaopeng Xiong, Yang-An Wen, Rachelle Fairchild, Yekaterina Y. Zaytseva, Heidi L. Weiss, B. Mark Evers, Tianyan Gao

Markey Cancer Center Faculty Publications

Colon tumors grow in an adipose tissue-enriched microenvironment. Locally advanced colon cancers often invade into surrounding adipose tissue with a direct contact with adipocytes. We have previously shown that adipocytes promote tumor growth by modulating cellular metabolism. Here we demonstrate that carnitine palmitoyltransferase I (CPT1A), a key enzyme controlling fatty acid oxidation (FAO), was upregulated in colon cancer cells upon exposure to adipocytes or fatty acids. In addition, CPT1A expression was increased in invasive tumor cells within the adipose tissue compared to tumors without direct contact with adipocytes. Silencing CPT1A abolished the protective effect provided by fatty acids against nutrient …

Functional Search Problems Among Medline Databases, C. Sean Burns

Information Science Presentations

The National Library of Medicine's (NLM) MEDLINE database is the most important bibliographic database in the bio and related sciences. MEDLINE is freely accessible via the PubMed web interface, but NLM also licenses MEDLINE data to information service providers including EBSCOhost, ProQuest, Ovid, and Web of Science. The talk covers a longitudinal study of these five MEDLINE-based bibliographic database platforms. The goal of the study is to identify where and how these platforms differ when searched with semantically and logically equivalent search queries. The results have implications for literature searches, including systematic reviews.

Preterm Birth Prevention In Appalachian Kentucky: Understanding Barriers And Facilitators Related To Transvaginal Ultrasound Cervical Length Surveillance Among Prenatal Care Providers, Anna Hansen, Mairead E. Moloney, Cynthia Cockerham-Morris, Jing Li, Niraj R. Chavan

Sociology Faculty Publications

Background: Appalachian Kentucky has higher-than-average rates of preterm birth (PTB)—a health disparity associated with increased maternal and fetal/neonatal morbidity and neonatal mortality. Transvaginal ultrasound (TVU) cervical length measurement is the best predictor of PTB risk, but is underutilized in Appalachia. This study explores prenatal care providers' TVU-related knowledge and practices, and identifies barriers and facilitators, which impact the adoption of this evidence-based technology.

Materials and Methods: This study recruited providers from three Appalachian Kentucky health care sites. Prenatal care providers took part in semistructured interviews and completed brief survey scales. Questions focused on PTB knowledge, TVU-related barriers, and suggestions for …

Suspected Malignant Hyperthermia And The Application Of A Multidisciplinary Response, Laura Ebbitt, Eric Johnson, Brooke Herndon, Kristina Karrick, Aric Johnson

Pharmacy Practice and Science Faculty Publications

Purpose: Malignant hyperthermia (MH) is a critical and potentially life-threatening emergency associated with inhaled anesthetic and depolarizing neuromuscular blocker administration. This is a single center’s response to MH. Summary: When signs of MH are observed, a page for “anesthesia STAT-MH crisis” is called, triggering a multidisciplinary response, including the deployment of a Malignant Hyperthermia Cart. The MH cart and the delegation of duties allows nurses, physicians and pharmacists to quickly understand their role in the stabilization, transition and recovery of a suspected MH patient. Conclusion: This case highlights the importance of multi-disciplinary involvement in these rare, but potentially fatal, cases.

Encoding The Odor Of Cigarette Smoke, Timothy S. Mcclintock, Naazneen Khan, Yelena Alimova, Madeline Aulisio, Dong Y. Han, Patrick Breheny

Physiology Faculty Publications

The encoding of odors is believed to begin as a combinatorial code consisting of distinct patterns of responses from odorant receptors (ORs), trace-amine associated receptors (TAARs), or both. To determine how specific response patterns arise requires detecting patterns in vivo and understanding how the components of an odor, which are nearly always mixtures of odorants, give rise to parts of the pattern. Cigarette smoke, a common and clinically relevant odor consisting of > 400 odorants, evokes responses from 144 ORs and 3 TAARs in freely behaving male and female mice, the first example of in vivo responses of both ORs and …

The Productivity And Hay Requirements Of Beef Cattle In A Year-Round Grazing System In North Cameroon, A. D. Nkengafac, P. Tinta, G. Ayuk, M. Mansue, F. Fuh

IGC Proceedings (1977-2023)

No abstract provided.

Multicentric Synchronous Giant Cell Granulomas Of The Mandible, Galal Omami

Oral Health Practice Faculty Publications

No abstract provided.

Misinformation More Likely To Use Non-Specific Authority References: Twitter Analysis Of Two Covid-19 Myths, Joseph Mcglynn, Maxim Baryshevtsev, Zane A. Dayton

Communication Graduate Research

This research examines the content, timing, and spread of COVID-19 misinformation and subsequent debunking efforts for two COVID-19 myths. COVID-19 misinformation tweets included more non-specific authority references (e.g., “Taiwanese experts”, “a doctor friend”), while debunking tweets included more specific and verifiable authority references (e.g., the CDC, the World Health Organization, Snopes). Findings illustrate a delayed debunking response to COVID-19 misinformation, as it took seven days for debunking tweets to match the quantity of misinformation tweets. The use of non-specific authority references in tweets was associated with decreased tweet engagement, suggesting the importance of citing specific sources when refuting health misinformation.

Opportunities To Use Secondary Plant Compounds To Manage Diet Selection And Gut Health Of Grazing Herbivores, Dean K. Revell, Andrew Kotze, D. T. Thomas

IGC Proceedings (1977-2023)

No abstract provided.

Mitochondrial Oxidative And Nitrosative Stress And Alzheimer Disease, D. Allan Butterfield, Debra Boyd-Kimball

Chemistry Faculty Publications

Oxidative and nitrosative stress are widely recognized as critical factors in the pathogenesis and progression of Alzheimer disease (AD) and its earlier stage, amnestic mild cognitive impairment (MCI). A major source of free radicals that lead to oxidative and nitrosative damage is mitochondria. This review paper discusses oxidative and nitrosative stress and markers thereof in the brain, along with redox proteomics, which are techniques that have been pioneered in the Butterfield laboratory. Selected biological alterations in—and oxidative and nitrosative modifications of—mitochondria in AD and MCI and systems of relevance thereof also are presented. The review article concludes with a section …

Trace Mineral Leaching From Equine Compost, Ashley L. Fowler, Mieke Brummer-Holder, Karl A. Dawson

Animal and Food Sciences Faculty Publications

Mineral leaching from compost can be environmentally disruptive. Little information is available regarding trace mineral leaching from equine-sourced compost. The objective of this study was to quantify the mineral content and leaching potential of compost produced from feces of horses fed different amounts and forms (organic or inorganic) of trace minerals. Nine horses were fed three treatments in a 3 × 3 replicated Latin Square design. The dietary treatments were provided as a daily pellet: CON (pellet without added trace minerals), ING (added inorganic trace minerals), and ORG (added organic trace minerals). The added trace minerals were Co, Cu, Mn, …

Everything Is Science: A Free City-Wide Science Festival, Jarrod W. Creameans, Michelle G. Pitts, Olivia White, Kellen M. Greenwell, Kristie Colón, Sylvie Garneau-Tsodikova, Vincent J. Venditto

Pharmaceutical Sciences Faculty Publications

A week-long, city-wide science festival called Everything is Science (EiS) was developed to educate the community in an informal manner. The festival serves as a platform for presenters from diverse professions to give engaging talks (without PowerPoint slides) to the public, free of charge, in restaurants and bars around town. Over 350 people attended the events over 5 days with 33 presenters. Surveys completed by attendees and session coordinators indicate strong support for this festival. Altogether, the EiS festival serves as a no-cost method to engage with the community and improve science literacy with potential for adoption in other cities.

Breaking Up The Grade, Thomas R. Guskey

Educational, School, and Counseling Psychology Faculty Publications

To make grading more meaningful, course grades should reflect a range of distinct criteria that make up student learning.

Lessons Learned From Six Different Structural Health Monitoring Systems On Highway Bridges, Abheetha Peiris, Charlie Sun, Issam E. Harik

Kentucky Transportation Center Faculty and Researcher Publications

Structural health monitoring has been utilized in numerous ways to investigate the performance and integrity of highway bridges. This paper highlights the use of six structural health monitoring systems, which were deployed to monitor distinct behaviors on six bridges in Kentucky. The structural health monitoring systems are as follows: (1) Over-height truck impact detection and monitoring on the I-64 over US 60 bridge, (2) Barge impact detection and monitoring on northbound US 41 over the Ohio River, (3) effectiveness of carbon fiber-reinforced polymer retrofit evaluation based on prestressed concrete I-girder crack movement on the I-65 elevated expressway in Louisville, (4) …

Staying Inspired In Difficult Times, Jennifer A. Bartlett

Library Faculty and Staff Publications

This issue's column suggests a few new releases to prompt professional reflection and renewal.

Teaching Undergraduates With Primary Sources 2020 Research Study Report, Jay-Marie Bravent, Deirdre Scaggs, Matthew Strandmark, Danielle Gabbard

Library Reports and White Papers

This report presents the findings of an exploratory examination of the pedagogical practices of social sciences and humanities instructors who teach undergraduates with primary sources at the University of Kentucky (UK). Conducted in December 2019 and January 2020 by a research team from the University of Kentucky Libraries Special Collections Research Center, the study reveals areas of success within existing programs and services, the benefits and drawbacks of teaching with digitized primary sources, as well as inherent pedagogical challenges to overcome. A list of recommendations based on the findings seeks to address these challenges and concludes the report. As part …

Occupancy And Abundance Of Stream Salamanders Along A Specific Conductance Gradient, Jacob M. Hutton, Steven J. Price, Simon J. Bonner, Stephen C. Richter, Christopher D. Barton

Forestry and Natural Resources Faculty Publications

In the Central Appalachians (USA), mountaintop-removal mining accompanied by valley fills often leads to streams with elevated specific conductivity (SC). Thus, the ionic composition of freshwaters in this region is hypothesized to be a driver of the distribution and abundance of freshwater taxa, including stream salamanders. We examined the association between SC and stream salamander populations by conducting salamander counts in 30 southeastern Kentucky streams across a continuous gradient of SC that ranged from 30 to 1966 μS/cm. We counted 2319 salamanders across 5 species and, using a hierarchical Bayesian version of the N-mixture model, found a negative association between …

Aluminum Reproductive Toxicity: A Summary And Interpretation Of Scientific Reports, Robert A. Yokel

Pharmaceutical Sciences Faculty Publications

Publications addressing aluminum (Al)-induced reproductive toxicity were reviewed. Key details were compiled in summary tables. Approximate systemic Al exposure, a measure of bioavailability, was calculated for each exposure, based on the Al percentage in the dosed Al species, Al bioavailability, and absorption time course reports for the exposure route. This was limited to laboratory animal studies because no controlled-exposure human studies were found. Intended Al exposure was compared to unintended dietary Al exposure. The considerable and variable Al content of laboratory animal diets creates uncertainty about reproductive function in the absence of Al. Aluminum-induced reproductive toxicity in female mice and …

Methods For Using Nhanes For Exposure Estimates, Heather R. Campbell, Robert A. Lodder

Pharmaceutical Sciences Faculty Publications

The National Health and Nutrition Examination Survey (NHANES) is a scheme of studies with the objective of measuring the health and nutritional status of adults and children in the United States. The survey is unique in that it has a clinical component (NCT00005154), and combines interviews and physical examinations into its data sets. NHANES is an important program of the National Center for Health Statistics (NCHS). NCHS is a unit of the Centers for Disease Control and Prevention (CDC) and has the responsibility for producing vital and health statistics for the United States. Data from the NHANES studies …

Afatinib For The Treatment Of Egfr Mutation-Positive Nsclc: A Review Of Clinical Findings, R. Donald Harvey, Val R. Adams, Tyler Beardslee, Patrick Medina

Pharmacy Practice and Science Faculty Publications

Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors represent the standard of care in patients with EGFR mutation-positive (EGFRm+) non-small cell lung cancer (NSCLC). The availability of several EGFR tyrosine kinase inhibitors approved for use in the first-line or later settings in NSCLC warrants an in-depth understanding of the pharmacological properties of, and clinical data supporting, these agents. The second-generation, irreversible ErbB-family blocker, afatinib, has been extensively studied in the context of EGFRm+ NSCLC. Results from the LUX-Lung 3 and 6 studies showed that afatinib was more active and better tolerated than chemotherapy in patients with tumors …

Intrarenal Renin Angiotensin System Imbalance During Postnatal Life Is Associated With Increased Microvascular Density In The Mature Kidney, Carolina Dalmasso, Alejandro R. Chade, Mariela Mendez, Jorge F. Giani, Gregory J. Bix, Kuey Chu Chen, Analia S. Loria

Pharmacology and Nutritional Sciences Faculty Publications

Environmental stress during early life is an important factor that affects the postnatal renal development. We have previously shown that male rats exposed to maternal separation (MatSep), a model of early life stress, are normotensive but display a sex-specific reduced renal function and exacerbated angiotensin II (AngII)-mediated vascular responses as adults. Since optimal AngII levels during postnatal life are required for normal maturation of the kidney, this study was designed to investigate both short- and long-term effect of MatSep on (1) the renal vascular architecture and function, (2) the intrarenal renin-angiotensin system (RAS) components status, and (3) the genome-wide expression …

A Smartphone-Based Support Group For Alcoholism: Effects Of Giving And Receiving Emotional Support On Coping Self-Efficacy And Risky Drinking, Woohyun Yoo, Dhavan V. Shah, Ming-Yuan Chih, David H. Gustafson

Health and Clinical Sciences Faculty Publications

The purpose of this study was to investigate the nature and effects of exchanging emotional support via a smartphone-based support group for patients with alcohol dependence. Of the 349 patients who met the Diagnostic and Statistical Manual of Mental Disorders (4th ed.) criteria for alcohol dependence, 153 patients participated in the discussion group within the Addiction-Comprehensive Health Enhancement Support System, a smartphone application aimed at reducing relapse. This was developed to prevent problem drinking by offering individuals in recovery for alcohol dependence automated 24/7 recovery support services and frequent assessment of their symptom status as part of their addiction care. …

Applying The Rasch Model To Evaluate The Self-Directed Online Learning Scale (Sdols) For Graduate Students, Hongwei Yang, Jian Su, Kelly D. Bradley

Educational Policy Studies and Evaluation Faculty Publications

With the rapid growth of online learning and the increased attention paid to student attrition in online programs, much research has been aimed at studying the effectiveness of online education to improve students’ online learning experience and student retention. Utilizing the online learning literature as a multi-faceted theoretical framework, the study developed and employed a new survey instrument. The Self-Directed Online Learning Scale (SDOLS) was used to examine graduate student perceptions of effectiveness of online learning environments as demonstrated by their ability to take charge of their own learning, and to identify key factors in instructional design for effective improvements. …

E-Cigarette Availability, Price Promotions And Marketing At The Point-Of Sale In The Contiguous United States (2014-2015): National Estimates And Multilevel Correlates, Heather D'Angelo, Shyanika W. Rose, Shelley D. Golden, Tara Queen, Kurt M. Ribisl

Behavioral Science Faculty Publications

Electronic cigarette (e-cigarette) sales and use have increased rapidly, yet point-of-sale e-cigarette availability and marketing is understudied. We estimated changes in e-cigarette availability and marketing among tobacco retailers in the U.S., and associations with neighborhood characteristics. A national sample of tobacco retailers in the Contiguous U.S. was audited in 2014 and 2015 (n = 1,905 and n = 2,126, respectively) to observe e-cigarette availability and marketing (signs, ads, displays and promotions) and generate national prevalence estimates. Store, neighborhood and state level correlates of 2015 e-cigarette availability, price promotions and exterior advertising were analyzed using multilevel mixed-effects generalized linear models. E-cigarettes …

Implementation Evaluation Of A Professional Development Program For Comprehensive School Physical Activity Leaders, Russell L. Carson, Ann Pulling Kuhn, Justin B. Moore, Darla M. Castelli, Aaron Beighle, Katie L. Hodgin, Brian Dauenhauer

Kinesiology and Health Promotion Faculty Publications

The purpose of this study was to conduct an implementation monitoring evaluation of a yearlong comprehensive school physical activity program (CSPAP) professional development program across eight multi-state physical education (PE) teacher cohorts. Mixed-method data were collected during a three-year implementation period via workshop attendance sheets and evaluations, post-workshop implementation plans and artifacts, and follow-up phone interviews to enumerate and evaluate the program’s process of recruitment, reach, dose delivered, dose received, fidelity, and context. Recruitment strategies reached a total of 234 PE teacher attendees across eight workshops, with 77 PE teachers (primarily female, elementary, public school teachers) completing all program requirements. …

Risk Factors For And Prediction Of Post-Intubation Hypotension In Critically Ill Adults: A Multicenter Prospective Cohort Study, Nathan J. Smischney, Rahul Kashyap, Ashish K. Khanna, Ernesto Brauer, Lee E. Morrow, Mohamed O. Seisa, Darrell R. Schroeder, Daniel A. Diedrich, Ashley Montgomery, Pablo Moreno Franco, Uchenna R. Ofoma, David A. Kaufman, Ayan Sen, Cynthia Callahan, Chakradhar Venkata, Gozde Demiralp, Rudy Tedja, Sarah Lee, Mariya Geube, Santhi I. Kumar, Peter E. Morris, Vikas Bansal, Salim Surani, On Behalf Of Sccm Discovery (Critical Care Research Network Of Critical Care Medicine) Hemair Investigators Consortium

Internal Medicine Faculty Publications

OBJECTIVE: Hypotension following endotracheal intubation in the ICU is associated with poor outcomes. There is no formal prediction tool to help estimate the onset of this hemodynamic compromise. Our objective was to derive and validate a prediction model for immediate hypotension following endotracheal intubation.

METHODS: A multicenter, prospective, cohort study enrolling 934 adults who underwent endotracheal intubation across 16 medical/surgical ICUs in the United States from July 2015-January 2017 was conducted to derive and validate a prediction model for immediate hypotension following endotracheal intubation. We defined hypotension as: 1) mean arterial pressure < 65 mmHg; 2) systolic blood pressure < 80 mmHg and/or decrease in systolic blood pressure of 40% from baseline; 3) or the initiation or increase in any vasopressor in the 30 minutes following endotracheal intubation.

RESULTS: Post-intubation hypotension developed in 344 (36.8%) patients. …

Hypusination Of Eif5a Regulates Cytoplasmic Tdp-43 Aggregation And Accumulation In A Stress-Induced Cellular Model, Shayna Smeltzer, Zainuddin Quadri, Abraian Miller, Frank Zamudio, Jordan Hunter, Nicholas J.F. Stewart, Sheba Saji, Daniel C. Lee, Dale Chaput, Maj-Linda B. Selenica

Sanders-Brown Center on Aging Faculty Publications

TAR DNA-binding protein 43 (TDP-43) is a nuclear RNA/DNA binding protein involved in mRNA metabolism. Aberrant mislocalization to the cytoplasm and formation of phosphorylated/aggregated TDP-43 inclusions remains the hallmark pathology in a spectrum of neurodegenerative diseases, including frontotemporal disorders and Alzheimer's disease. Eukaryotic Translation Initiation Factor 5A undergoes a unique post-translation modification of lysine to hypusine (K50), which determines eIF5A binding partners. We used a sodium arsenite-induced cellular stress model to investigate the role of hypusinated eIF5A (eIF5A^HypK50) in governing TDP-43 cytoplasmic mislocalization and accumulation in stress granule. Our proteomics and functional data provide evidence that eIF5A interacts …