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Fleshcrawl, Shanita Jackson

Theses and Dissertations--English

I am fascinated with the spiritual, physical, and psychological experiences that occur when one’s flesh crawls. The experiences, akin to inverted goosebumps, result from provocations of the senses or of the mind. James Blake’s latest album, for example, triggers my flesh to crawl. The art on the album cover depicts a decaying body turned into a canyon-like labyrinth. In undergrad, attempts to conceptualize slave-deck diagrams disturbed my flesh to crawl. I am intrigued by how anxiety, epigenetics, and superstition all factor into the subjective intensities of this collective experience. As it seems, circumstance heightens the severity and frequency of the …

Investigating The Role Of Chd7 And Sox11 In Retinal Cell Development And The Ocular Complications Of Charge Syndrome, Laura Krueger

Theses and Dissertations--Biology

Proper formation of the visual system requires the precise interaction of several embryonic cell lineages, including the neuroectoderm (forms the retina and retinal pigment epithelium), surface ectoderm (forms the lens), mesoderm and cranial neural crest cells (form the ocular blood vessels and anterior ocular structures). When this process is disrupted structural birth defects such as coloboma result, leading to pediatric visual deficits. Ocular developmental defects are often present in larger syndromic disorders. One example is CHARGE syndrome, a genetic disorder characterized by coloboma, heart defects, choanal atresia, growth retardation, genital abnormalities, and ear abnormalities. Pathogenic variants in CHD7 have been …

Metabolic Diversity Among Fibroblasts From Regenerating And Non-Regenerating Mammals, Ebenezer Aryee

Theses and Dissertations--Biology

Regeneration is a wound repair process that terminates in the restoration of tissue function and structure. Fibroblasts play pivotal roles in regenerative and fibrotic wound repair. Reports of extensive regenerative ability in mammals have been historically rare, but more recently spiny mice (Acomys) have emerged as a bona fide model of complex tissue regeneration. Recent work has indicated that fibroblasts from regenerators (Acomys and Oryctolagus) are more resistant to reactive oxygen species (ROS)-induced senescence compared to non-regenerating species, suggesting the influence of intrinsic cellular states on the fate of wound repair. Determining the basal metabolic signature …

Estrogen Regulation Of Daily Metabolic Rhythms In Female Mice, Oluwabukola Omotola

Theses and Dissertations--Biology

Circadian rhythms are approximately 24-hour cycles of behavior, physiology, and gene expression. In mammals, these circadian rhythms are generated by clocks located in nearly every tissue in the body. The function of circadian clocks is to synchronize physiology and behavior with environmental cycles such as the light-dark cycle. After menopause, when circulating levels of estrogens are very low, women are more susceptible to obesity comorbidities such as metabolic syndrome and cardiovascular disease, suggesting that estrogens regulate these processes. Estrogens could protect females from metabolic dysfunction by regulating circadian rhythms. Consumption of diets that are high in fat contribute to obesity. …

Naturally Occurring And Introduced Tracers For Examining Water Pathways In Urban Environments, Elizabeth Avery

Theses and Dissertations--Earth and Environmental Sciences

Naturally occurring stable isotopes of water and introduced water tracers allow researchers to examine water pathways and better understand spatial and temporal variability in water sources. Trends in naturally occurring stable isotope values can function not only as a tracer for precipitation patterns and moisture recycling but also as a confirmation of municipal data. Additionally, these data can provide an early signal for the effects of climate change on these sources, reducing uncertainty from physical measurements. To further assess water pathways, introduced tracers can be used to investigate surface and below ground surface flow for streams and rivers.

In chapter …

The Role Of Sox4 In Ocular Morphogenesis And Retinal Differentiation, Rebecca Petersen

Theses and Dissertations--Biology

Visual impairment ranges from mild forms that can be corrected with glasses to more severe cases that result in permanent loss of vision. Microphthalmia, anophthalmia, and coloboma (collectively referred to as MAC) account for 11% of cases of pediatric blindness and are a result of improper ocular morphogenesis. Retinitis Pigmentosa (RP) is a retinal degenerative disease that affects 1 in 3000 people worldwide. It is a progressive disorder that initially begins with loss of vision in low light settings due to rod photoreceptor degeneration but progresses to complete blindness upon loss of cone photoreceptors. Currently, there is no cure for …

Gender And Remittances: Lived Experiences Of Women In Oaxaca, Mexico, Araby Smyth

Theses and Dissertations--Geography

This dissertation project analyzes the ways that migration and remittances, the money that migrants send to people in their place of origin, intersect with the political and social dynamics in an Indigenous community in the Isthmus of Tehuantepec region of Oaxaca, Mexico. Drawing on 16 months of ethnographic fieldwork, which included semi-structured interviews and participant observation alongside historical archival investigation, this dissertation examines the following questions: What international organizations, national government, and private sector policies govern remittances? How does Indigenous collective work and communal governance shape remittance management? How do the responsibilities of family members shift with migration and how …

Undoing Colorblind Ecologies: Redlining And Just Green Enough In The Urban Forest Of Boston's Franklin Park, Chelsea M. Parise

Theses and Dissertations--Geography

Urban political ecology research increasingly engages multi-disciplinary methodologies to clarify the role that the botanic plays in creating, maintaining, or subverting ecological geographies of power. Fredrick Law Olmsted intended the forest within Franklin Park to heal the physical degeneration and social disunity he believed resulted from urban living conditions but instead the forest within Franklin Park has grown in contexts of increasingly complex environmental and racial difference. I examine how the urban forest in Boston’s Franklin Park has ecologically manifested racialized power relations through distinct periods of elite nature-making and segregated grassroots stewardship. I utilized archival research, forest surveys, and …

Tracks/Traces: The New Deal Transformation Of Lexington, Kentucky’S Landscapes Of Horseracing And Housing, Piotr Wojcik

Theses and Dissertations--Geography

Lexington, Kentucky is a key node in the global thoroughbred horse industry. This archival research examines the transformation of its horseracing and housing geographies during the 1930s by comparing the redevelopment of an old urban racetrack into federal public housing with the simultaneous development of a new racing plant in the nearby countryside. It analyzes the social and economic relations underlying this shift in addition to how these relations were naturalized by the new landscapes they created. Results suggest that a local growth coalition was seeking to emerge from a financial crisis through a spatial fix that capitalized on cultural …

Elephants In The Room: Covid-19 Pandemic Political Ecologies Of Tourism In Tanzania, Helen C. Richardson

Theses and Dissertations--Geography

The COVID-19 pandemic brought forth unprecedented and ever-changing crisis and disruption to societies and economies around the globe.[1] As the COVID-19 pandemic continues to interrupt travel worldwide, the tourism industry, and the countries who rely on it as a major source of income, are in crisis. These processes have reconfigured economic capital flows and foreign investment in the global south. This is particularly the case in Tanzania, as tourism was Tanzania’s highest foreign exchange earner and accounted for 17% of Tanzania’s gross domestic product in 2019.[2] This project draws upon a political ecology framework to examine the Tanzanian …

Speaking Of Home Here And There: Everyday Experiences Of Belonging Among Highly Educated Immigrants, Katherine Feske-Kirby

Theses and Dissertations--Geography

This thesis explores how highly educated immigrants articulate a sense of belonging upon relocating to the United States, more specifically to the Lexington, KY area. Engaging with feminist political geography as well as migration and cultural studies, I argue that articulations of belonging are framed through transnational attachments, which respectively expand individuals’ ability to employ everyday forms of belonging. Expressions and understandings of transnational belonging are framed through in-depth interviews on participants’ workplace, relational dynamics, and engagement with the geopolitical discourse on migration. Through these interviews, a broader representation belonging is presented, while questions on highly educated immigrants’ privilege and …

Part-Time Normals: Embodied Trans Geographies Of Homonationalism, Ivy Faye Monroe

Theses and Dissertations--Geography

Self-understanding of one’s gender identity both emerges from, and rearticulates into, the ways one experiences and mediates their personal and social relationships with the geographic worlds they inhabit. Trans geographical literature has, to date, created compelling work on the social geographies of trans people in highly-gendered spaces. This thesis extends the existing literature to research how gender is both experienced and performed in the mundane structures of everyday life. Building from theories of cruel optimism and homonationalism, this research examines how the discursive and spatial epistemologies of gender identity inform attachments to structures of normativity. Through archival research of transvestite …

First-Person Politics: Strategies Of Latin/X American Women To Change The Neoliberal Requirements For Empowerment And Inclusion One Share, Like, Subscribe At A Time, Marlee Northcutt

Theses and Dissertations--Hispanic Studies

This project investigates the strategies of Latin/x American women who have used their voices and influence in the media to break barriers, enter spaces that have excluded them, and advocate for changes so that young girls like them do not have to face these same limitations. Chapter One investigates politicians who, from their political power positions, interweave personal stories with their accomplishments to provide role models for these careers. Chapter Two identifies actors who combine their personal stories with activist causes to alter representation in TV and film. The YouTubers in Chapter Three bolster a rhetoric of empowerment to encourage …

Colonial Contraception: American Birth Control Advocates And Their Work In Appalachia, Puerto Rico, And India; 1930-1970, Dana Johnson

Theses and Dissertations--History

By the beginning of the twentieth century, the development of better contraceptives and changing cultural attitudes led to an increased interest in contraceptive research. Although major political, legal, social, religious, and cultural obstacles remained, birth control advocates began to perform clinical trials to identify effective contraceptives and to disseminate contraceptive information. These trials began in the United States, but birth control advocates quickly introduced them into other areas. In this dissertation, I examine the research efforts of the American birth control movement through an analysis of the activities and discourse of its key advocates and promoters during the middle decades …

The Role Of Sexual Difference In Plato's Timaeus, Mary Cunningham

Theses and Dissertations--Philosophy

My dissertation is a reading of Plato’s Timaeus that centers sexual difference and in particular femininity. I analyze the role of sexual difference in the framing of the dialogue as well as its accounts of body in the first and second discourse and its account of health in the third discourse. I argue that sexual difference, and, in particular, sexual reproduction, serves as a guiding paradigm of Timaeus’ entire project. I argue in each part of my dissertation that various aspects of the Timaeus depend on a certain notion of sexual difference—even aspects which are seemingly causally prior to the …

The Impacts Of Public Policies On Health-Related Outcomes, Yaxiang Song

Theses and Dissertations--Economics

This dissertation consists of three chapters that study how public policies affect health-related outcomes in China. The first two papers examine the impacts of the country’s Critical Illness Insurance program on health-related outcomes. The third paper studies the effects of maternal education on children’s early childhood health outcomes and cognitive skills.

The first chapter evaluates the impact of a recent social health insurance benefits expansion program on middle-aged and elderly individuals. In 2012, China launched a Critical Illness Insurance (CII) program, which aimed to reduce out-of-pocket costs for individuals covered by the government’s health insurance programs. This chapter utilizes the …

Safety In The Woods: Studying The Effectiveness Of The Kentucky Master Logger Program, Austin Winn Leake

Theses and Dissertations--Community & Leadership Development

Logging is a dangerous field; in this study the researcher looks to see the knowledge both before and after professional loggers participate in the Kentucky Master Logger Program. This information was used to see the overall effectiveness of the Kentucky Master Logger Program. The researcher found an overall improvement in logging professionals’ perceptions and understanding of logging safety. The researcher recommends creating an emphasis on a safe work culture through safety incentives and a greater focus on less used personal protective equipment such as insecticide

Significant Life Experiences Of Kentucky Youth Climate Activists, Jeri Katherine Howell

Theses and Dissertations--Community & Leadership Development

The purpose of this study is to better understand Kentucky youth climate activism. The research questions explore how youth define their climate activism in Kentucky, their Significant Life Experiences (SLE) that motivated them to commit to climate activism, and what challenges and sustains their involvement. This qualitative study utilizes a blended framework of social/environmental positionality and political ecology to analyze 7 semi-structured interviews with participants between the ages 18 to 24 years old who are acting to address climate change in Kentucky. New contributions to the existing body of SLE literature are discussed.

The Resiliency And Thriving Of Underrepresented Agricultural Educators: A National Mixed Methods Study, Caleb Michael Hickman

Theses and Dissertations--Community & Leadership Development

Gay men in agricultural education do not have comprehensive support within the agricultural education profession. When gay men decide to become agriculture educators, they often keep their identity private. This national mixed methods study aims to seek if gay agriculture educators are resilient and thrive in rural communities. The thriving elements of spiritual influence, personal competence, peer support, and family cohesion were surveyed and analyzed using a resiliency lens. Findings include gay male agricultural educators thriving in a heteronormative profession. Recommendations include ensuring LGBTQIA+ teachers have a voice in agricultural education.

Examining Social Mission In Kentucky Social Enterprises, Bo Milburn

Theses and Dissertations--Community & Leadership Development

Social Enterprises are business entities that incorporate social value creation into their business operations. This study aims to investigate, explore and share key insights gained through the lived experience of owners or managers of these organizations. The reputational method of sample selection was used to identify informed individuals who participated in interviews to share phenomenological knowledge by telling the stories of their organizations, describing how they manage the balance between profitability and sustainability, sharing observations about the influence of social mission at their firms and other insights. Participants identified stronger relationships between coworkers, increased professional creativity, and greater employee engagement …

The Youth - Adult Partnership Between Secondary Agriculture Educators And Native American Youth: A Qualitative Case Study, Hunter Anne Julian

Theses and Dissertations--Community & Leadership Development

This case study examines the connection made between secondary agriculture educators through Youth-Adult Partnerships (YAP). This study provides insight into the elements of YAP and contributes to the limited body of research surrounding Youth-Adult Partnership in secondary agriculture education and in the Native American community. Data was collected through qualitative interviews of six agriculture educators teaching on Native American reservations. The findings of this study indicate that YAP is present in secondary agriculture education programs and offers insight into how agriculture educators form connections with the Native American communities they teach in.

Membrane Palmitoylated Protein Magu-3 Regulates The C. Elegans Locomotor Circuit Via Modulation Of Cholinergic Activity, Henry Richburg

Theses and Dissertations--Medical Sciences

Precise coordination of the activity and transmission in excitatory and inhibitory neural circuits is essential for healthy information flow. Synaptic scaffolding proteins play a key role in the regulation of circuit activity through the localization and organization of receptors, channels, and other synaptic machinery to facilitate signal transduction. Using the C. elegans motor circuit which has both cholinergic (excitatory) and GABAergic (inhibitory) inputs onto muscles, we revealed an unexplored role of a family of scaffolding molecules known as membrane palmitoylated proteins (MPPs). Here, we examined magu-3, a C. elegans ortholog within the membrane palmitoylated protein family, using GFP tagging …

Investigating The Effects Of In-Vivo Therapeutics Hypoxia Treatment Paradigms In Neurite Outgrowth Patterns, Jae Hyun Yoo

Theses and Dissertations--Medical Sciences

Spinal cord injury, specifically in the cervical C3-C4 region of the cervical spine, contributes to impaired breathing and a diminished quality of life. Therefore it is important to find effective and safe therapeutics to restore breathing function. Indeed, there are a myriad of research being performed in addition to valuable collaboration amongst different institutions. As such, inspired by a previous experiment, we decided to test our hypothesis that an enriching environment consisting of different hypoxic environments - sustained and intermittent hypoxia alongside normoxia - would result in neurite outgrowth. Moreover, we hypothesized that sustained hypoxia would result in the greatest …

Mammalian Target Of Rapamycin Cell Signaling Pathway In Phosphatase And Tensin Homolog Induced Kinase 1 Knockout Rat Model Of Familial Parkinson's Disease, Martha Helena Mortell

Theses and Dissertations--Medical Sciences

More than 10 million people are living with Parkinson’s disease (PD), one million of which are people in the United States. PD is the second most common age-related neurodegenerative disorder, after Alzheimer’s disease, and is characterized by the accumulation of a-synuclein aggregates and the degeneration of dopaminergic neurons. The loss of endogenous dopamine in PD brain accounts for the motor decline presented clinically in PD patients. Etiological factors of PD include oxidative damage and inflammation, although the detailed mechanisms remain unknown. Risk factors for PD include gender, age, environmental factors, and gene mutations.

The current thesis research employed phosphatase and …

Trem2: Gene Expression And Role In Alzheimer’S Disease, Henry Snider

Theses and Dissertations--Medical Sciences

Genetic mutations in the TREM2 gene are highly correlated with risk of Alzheimer’s disease, but alternative splicing patterns of TREM2 transcripts have not been fully described. Characterization of TREM2 alternative splicing will be of significant use to the scientific community as the field of Alzheimer’s disease research progresses. The goal of this study was to fully describe splicing patterns in TREM2, as different splicing isoforms of genes can alter express and/or function of the final protein. Human blood and anterior cingulate cortex brain tissue from 61 individual donors was processed and used for PCR and quantitative PCR as well as …

Apoe Genotype And Sex Modulate Ketogenic Diet Enhancements To Metabolism And Gut Microbiome In Young Mice, Andrew T. Yackzan

Theses and Dissertations--Medical Sciences

The apolipoprotein ε (APOE) allele in humans has been associated with risk for development of Alzheimer’s disease (AD). There are predominately three variations of the allele – ε2 (E2), ε3 (E3), and ε4 (E4) – with E4 contributing the greatest risk of AD development. Recent research has unveiled evidence of neurometabolic and neurovascular deficits in E4 carriers present decades before the onset of dementia; it is believed these chronic defects play a major role in the development of AD, thus making them a potential target for preventative intervention. The purpose of this study was to examine the effect of a …

Classification And Effect Of Correctors On Sitosterolemia-Associated Mutants In Abcg8, Brittney Poole

Theses and Dissertations--Medical Sciences

Objective: To classify mutants of ABCG8 identified in subjects with clinically confirmed Sitosterolemia, a rare form of Familial Hypercholesterolemia distinguished by the accumulation of phytosterols in plasma and tissues and determine the effects of correctors and/or regulators of proteostasis on maturation of the ABCG5/ABCG8 sterol transporter.

Methods: Disease-causing missense mutants within the cytosolic domain of ABCG8 were generated through site-directed mutagenesis. Normal and mutant proteins were expressed in human hepatocytes. Cellular proteins were prepared, and maturation was assessed by SDS-PAGE and immunoblotting. Formation of the higher molecular weight, mature form of glycoproteins was used as a bioassay for trafficking the …

Evaluating The Microbiome To Boost Recovery From Stroke: The Embrs Study, Tyler Hammond

Theses and Dissertations--Neuroscience

Accumulating evidence suggests that gut microbes modulate brain plasticity via the bidirectional gut-brain axis and may play a role in stroke rehabilitation. A severely imbalanced microbial community has been shown to occur following stroke, causing a systemic flood of neuro- and immunomodulatory substances due to increased gut permeability and decreased gut motility. Here we measure post-stroke increased gut dysbiosis and how it correlates with gut permeability and subsequent cognitive impairment.

We recruited 12 participants with acute stroke, 12 healthy control participants, and 18 participants who had risk factors for stroke, but had not had a stroke. We measured the gut …

Evaluating The Role Of Mmp9 In Hyperhomocysteinemia Induced Cerebrovascular Pathology, Alexandria Linton

Theses and Dissertations--Neuroscience

Vascular contributions to cognitive impairment and dementia (VCID) is one of the leading causes of dementia, along with Alzheimer’s disease (AD), and often manifests as a comorbidity of AD. Elevated plasma homocysteine, known as hyperhomocysteinemia (HHcy), is a risk factor for VCID; however, the mechanism underlying the connection between HHcy and development of VCID pathology remains elusive. Understanding this mechanism would reveal novel therapeutic targets with the potential to be disease modifying, which are a critical need for a disease that lacks any approved treatment. Previous studies from our lab have strongly associated neuroinflammation and blood brain barrier (BBB) dysregulation …

Peripheral And Central Glucose Flux In Type I Diabetes, Jelena Anna Juras

Theses and Dissertations--Neuroscience

Diabetes is a complex metabolic disorder, of which high blood glucose concentration is the primary hallmark. Type I diabetes mellitus (T1DM) is characterized by the lack of insulin production, due to a poorly understood autoinflammatory cascade. In the words of historian Barnett “Diabetes may no longer be a death sentence, but for more and more people in the 21st century, it will become a life sentence”, making it the focal point of many research groups. It is estimated that around 20 million individuals worldwide live with T1DM.

Effects of long-term chronically elevated blood glucose are not only seen in micro/macro-vascular …