Digital Commons Network^™

Solving The Healthcare Employee Shortage: The Effectiveness Of Incentive Programs In Enticing Young Health Professionals To Appalachian Kentucky Communities, Taylor Williams

MPA/MPP/MPFM Capstone Projects

Kentucky’s Appalachian counties have long been recognized as medically underserved areas, with access to care being limited by a shortage of healthcare professionals in the region. This issue, specifically as it pertains to primary care physicians, has been the focus of many programs hoping to improve access to care in these communities for many years. Is the ongoing effort to recruit and retain primary care physicians in Appalachian Kentucky counties effective? Hypotheses The mean number of people per physician in Appalachian Kentucky counties reported in 2013 is equal to the mean number of people per physician in Appalachian Kentucky counties …

Cares Act Unemployment Insurance Program Implementation In Kentucky: A Case Study, Alicia Landon

MPA/MPP/MPFM Capstone Projects

In March of 2020, the COVID-19 pandemic arrived in the United States. The deadly virus filled hospitals to capacity and caused approximately 375,000 deaths nationwide over the next nine months (Ahmad et al., 2021). In an effort to slow the spread of the Coronavirus, federal and state governments closed businesses and agencies within a matter of weeks. Almost as quickly as the threat to human health changed the lives of Americans, the secondary threat of economic disruption began to unfold. Nationwide unemployment rates increased from approximately 4% to 15% between February 2020 and April 2020 (U.S. Bureau of Labor Statistics, …

Examining Academic Performance And Education Stability Measures For Youth In Foster Care, Alex Asbury

MPA/MPP/MPFM Capstone Projects

The state of Kentucky has seen an increase in its population of youth who are involved with child welfare and in the foster care system while also seeing less cases end in reunification with families. While youth process trauma related to the removal from their homes and the potential of experiencing multiple moves through their time with child welfare, it is important that educational stability remain intact to promote normalcy and improve educational outcomes for youth in foster care. Following the signing of the federal Every Student Succeeds Act (ESSA), the Kentucky education system has seen improvement in its processes …

The Effect Of Evidence-Based Funding On Student Performance, Jade Gates

MPA/MPP/MPFM Capstone Projects

Historically, education funding policies in the United States have been responsible for aiding the disproportionate allocation of resources to public school districts. Illinois school districts in particular have struggled with providing their students with an adequate education. The Evidence-Based Funding (EBF) model was implemented with the intent to distribute funds based on need and ability to reach an adequacy target. This research analyzes how student test scores have changed since the inception of the EBF policy in Illinois. This data is analyzed in an ordinary least squares and interrupted time series model to show the impact and significance of this …

Expanding The Samat In Kentucky Correction Institutions: Benefits And Funding, Blair Lozier

MPA/MPP/MPFM Capstone Projects

The United States has seen an increasing rate of substance use disorder among individuals. Those convicted and incarcerated are more likely to have substance use disorder. With correctional institutions becoming overcrowded, policymakers struggle to find an answer to the number of individuals incarcerated with substance use disorder. The Cabinet of Health and Family Services and the Department of Corrections created Supportive Assistance with Medication for Addiction Treatment (SAMAT) in Kentucky. SAMAT is an addictive services program focused on preventing overdose and relapse and aiding recidivism for individuals with opioid and alcohol use disorder. The SAMAT has been deemed successful through …

Public Health Access And Service For All, Austin Nugent

MPA/MPP/MPFM Capstone Projects

Collecting, analyzing, and reporting accurate, reliable data is necessary for achieving health equity. Although data alone will not lead to equitable health outcomes for all people, it helps health professionals, researchers, and policymakers understand health disparities and develop effective interventions, programs, and policies to reduce them. Necessary data includes disaggregated health information on diverse demographics, including people with disabilities. However, despite this need, disability data gaps exist. Fortunately, numerous solutions exist to address this issue, including implementing minimum data standards on disability and engaging all levels of health organizations in data practices. This capstone project investigated the current data practices …

The Financial Impact Of Undocumented Workers On Kentucky's Economy, Diana Gold

MPA/MPP/MPFM Capstone Projects

Undocumented immigrant workers have a significant presence and impact on the economy of the Commonwealth of Kentucky. However, it is difficult to fully understand their financial impact due to the scarcity of published statistics on this topic. In addition, undocumented immigrants try to live unnoticed due to the nature of their legal status and fear of deportation. Some nonprofit organizations and a few public institutions have been able to track data related to their tax contributions, population size, ages, work fields, spending habits, and public resources used. Gathering the available data will help better understand the financial impact of undocumented …

A Study On Motivators And Satisfaction In Arts And Culture Public Programs: Implications For Policy Implementation, Eunyoung Yi

MPA/MPP/MPFM Capstone Projects

This study examines individuals’ motivations related to their participation in arts and culture activity programs and their support for the active roles of the government in cultivating such programs in South Korea. In doing so, this study suggests policy implications that help policymakers and stakeholders develop effective arts and culture programs in the public sector.

This study claims that redesigning the program contents reflecting sociopsychological motivations, expanding the policy target to the general public, and embracing the concept of lifelong arts and culture education will ensure inclusive accessibility and promote lifelong learning opportunities for all citizens.

Guided by intrinsic and …

A Study On Characteristics Associated With Unmet Adl Needs Among People With Disabilities In Korea, Sun-Gill Jung

MPA/MPP/MPFM Capstone Projects

Long-term care services are assistance provided to people with disabilities needing help with ADL (activities of daily living). This study aims to address two research questions regarding people with disabilities in Korea who have ADL needs: 1) who is served and not served by long-term care services from the government, and 2) what characteristics are associated with unmet ADL needs among those who are not served by long-term care services. To answer these questions, this study utilizes data from the supplementary survey of people with disabilities in the 2020 Korea Welfare Panel and conducts descriptive statistics and multiple regressions. The …

And The Survey Says ... A Qualitative Exploration Of Structurational Divergence From The Perspectives Of Nurse Managers Who Are Accountable For Patient Experience Measures, Lisa Carpenter Huddleston

Theses and Dissertations--Communication

For more than a decade, hospital leaders have focused on boosting patient experience scores as part of the federal government’s value-based purchasing (VBP) program. Hospitals that receive federal financial assistance (such as Medicare) are mandated to participate in the Hospital Consumer Assessment of Healthcare Providers and Systems (HCAHPS), a standardized survey that measures patients’ perceptions of their care. Results are publicly reported, and hospitals may be penalized on their reimbursements if they do not reach established benchmarks for patient experience. However, much debate has occurred about whether VBP has increased the quality of healthcare and whether the HCAHPS is an …

Secondary Traumatic Stress And Women Music Educators: A Phenomenological Study, Abigail Van Klompenberg

Theses and Dissertations--Music

Secondary traumatic stress (STS) is a phenomenon that affects individuals in helping professions, including educators. STS is a trauma disorder and results from one’s exposure to others experiencing trauma or who have experienced trauma. STS can affect physical health, well-being, and professional performance. Due to the profound effects of STS, there is a need for continued research to clarify this phenomenon in educators, specifically music educators. Furthermore, there is a need for research on STS in women due to women’s unique interactions with trauma and the potential for them to develop trauma disorders. At the time of this study, there …

Music Therapy At An Inclusive Early Learning Center: A Grant Proposal, Laura Elliott Buckner

Theses and Dissertations--Music

Music in early childhood can enhance the development of communication, language, cognitive, and social emotional skills. Music therapy administered by a board-certified music therapist can be tailored to fit the specific developmental needs of children with and without disabilities. The purpose of this comprehensive clinical project was to develop a music therapy program for the Early Learning Center at the Lexington Hearing and Speech Center and a grant proposal to fund this program. First, I piloted a mini program through my Graduate Clinical Placement in Music Therapy (MUS 633) and supervisory experiences through my teaching assistantship. I then developed a …

Polyrhythmic Pathways: Using Bimanual Coordination Research To Develop A New Framework For Practice, Performance, And Pedagogy, Christian Swafford

Theses and Dissertations--Music

This study reviews and compares percussion literature pertaining to polyrhythms and scientific literature pertaining to bimanual coordination. There exists a gap in the pedagogical approach to polyrhythms, and there is much disagreement between common instructional methods, especially when considered against the findings of several bimanual coordination studies. The purpose of this study is to reveal insight to the percussion community that the learning of polyrhythms is facilitated by the brain in novel ways, and the uniqueness of this learning process requires a rethinking of the current pedagogical approach. Percussion articles, method books, popular literature, and music scores are surveyed alongside …

Exploring Cary Boyce's 'Kosmos', Kristina Ploeger-Hekmatpanah

Theses and Dissertations--Music

A text with many layers of depth married to music with a variety of methods of interpretation allows for an academically satisfying learning, rehearsal, and performance process. Choral directors, and the choristers they serve, enjoy scrutinizing all the ways they feel connected to or disconnected from the text and music in any piece that proves worth their efforts. American composer Dr. Cary Boyce’s (b. 1955) Kosmos (2007), a setting of a poem from Walt Whitman (1819-1892) of the same name, indeed provides the substance an academic group desires. At choral festivals in the Pacific Northwest during the 2021-2022 academic year, …

Winter Rye (Secale Cereale L.) Management And Production Profitability In Kentucky, And Heritability Of Sensory Attributes, Elzbieta Szuleta

Theses and Dissertations--Plant and Soil Sciences

Winter rye (Secale cereale L.) is a small grain crop characterized by outstanding tolerance to drought, low temperatures, soil salinity and relatively low nutritional requirements. Rye is widely used as a cover crop, well known for its deep root system and outstanding nitrogen scavenging ability. This crop is widely used as a feed for livestock but also it is beneficial in human nutrition. Because of its versatile use, nutritional benefits and unique aroma and flavor, especially for bread and whiskey, there is an increasing interest in this crop in the United States. Unfortunately, rye grain production in the United …

Trait Impulsivity And Acute Alcohol Responses As Markers Of Binge Drinking In Young Adults, Holley Jamison

Theses and Dissertations--Psychology

Impulsivity, tolerance to alcohol-induced motor impairment, and high sensitivity to alcohol-induced disinhibition each have empirical support as markers of alcohol-related problems. However, research on alcohol response and personality-based markers of risk have remained largely independent lines of research. This dissertation examined how individual differences in these well-established markers may interact to additively contribute to heavy drinking.

Participants were 96 young adults (21-33 years old). with no history of AUD. They self-reported their drinking habits over the prior 90 days, and behavioral tasks measuring disinhibition and motor impairment were administered on two separate days following two alcohol doses (active [0.65 g/kg] …

Sugar And Spice: Sex, Money, And Social Media, Rachel Elizabeth Davis

Theses and Dissertations--Sociology

Interest in transactional sex, or the provision of a sexual relationship in exchange for gifts and/or money, has increased in recent years among researchers, nongovernmental organizations, and law enforcement officials as increasing numbers of women self-identify as hypergamous, indicating their interest in forming heterosexual partnerships with men of higher status. Hypergamous women may identify as sugar babies, spoiled girlfriends, or high-value women. A sugar baby is a woman providing romantic companionship to an older man, known as a sugar daddy, in exchange for money and/or gifts. A spoiled girlfriend is a woman whose partner provides her with money and/or gifts …

The Conditions Of Oregon's Latinx Farmworkers, Kevin Alejandrez

Theses and Dissertations--Sociology

The goal of this three-paper dissertation is to better understand inequalities that Latinx farmworkers endure and the ways through which these inequalities can be addressed. As such, this dissertation examines pertinent inequalities Latinx farmworkers experience in the United States, their responses to resulting hardships, and the effects that crises such as the Covid-19 pandemic have in both exacerbating hardship and expanding opportunities to challenge inequitable systems in place. This is done through an intersectional analysis of a multi-year ethnographic study conducted in Oregon’s Willamette Valley.

First, paper one, titled, “Subordinate Adaptation: Intragroup Hierarchies Among Blueberry Pickers,” explores the ways in …

Parenthood, Mental Health, And Intergenerational Adversity: Exploring Gender Role Ideologies, Household Composition And Work-Family Conflict, Ashley Vowels

Theses and Dissertations--Sociology

To date, research at the intersections of parenting, stress, gender, and mental health has yielded complicated and inconsistent results. The objective of this dissertation was to deepen our understanding of how parenting stress contributes to the mental health of both parents and their children, especially within the context of balancing work and family responsibilities. A three-study approach was used to assess differences by sex, gender role ideology, and household composition. Additionally, it explored the impact of parents’ characteristics on their children’s well-being in adulthood.

This project utilized secondary data from the Panel Study of Income Dynamics (PSID). Multivariate regression analyses …

A Characterization Of Key Residues In Class I Viral Fusion Proteins Important For Fusogenic Activity, Hadley E. Neal

Theses and Dissertations--Molecular and Cellular Biochemistry

Viral fusion proteins are critical for viral entry and subsequent infection. Class I fusion proteins are characterized by synthesis as an inactive precursor requiring cleavage by a host cell protease to become fusion competent. Though vaccine and antiviral therapeutic developments often target the fusion protein, questions surrounding cleavage dynamics and protein stability remain. The work presented in this dissertation investigates specific regions of three class I viral fusion proteins in an effort to identify key residues involved in proteolytic processing and membrane fusion.

The trimeric severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S) mediates receptor binding, facilitates fusion …

Characterization Of The Function And Regulation Of The Hmpv Phosphoprotein, Rachel Thompson

Theses and Dissertations--Molecular and Cellular Biochemistry

Human metapneumovirus (HMPV) is a non-segmented, negative strand RNA virus (NNSV) that frequently causes respiratory tract infections in infants, the elderly, and the immunocompromised. Despite the initial identification of HMPV in 2001, there are currently no FDA approved antivirals or vaccines available. Therefore, understanding the mechanism of HMPV replication is critical for the identification of novel therapeutic targets. A key feature in the replication cycle of HMPV and other NNSVs is the formation of membrane-less, liquid-like replication and transcription centers in the cytosol termed inclusion bodies (IBs). Recent work on NNSV IBs suggests they display characteristics of biomolecular condensates formed …

Advancements In Glycogen Detection And Spatial Profiling Reveals A New Therapeutic Path For Ewing Sarcoma: A Non-Traditional Glycogen Storage Disease, Lyndsay E. A. Young

Theses and Dissertations--Molecular and Cellular Biochemistry

Glycogen is the primary glucose storage carbohydrate in mammals, and it is synthesized in most tissues. As glycogen architecture modulates its role in metabolism, it is essential to accurately evaluate and quantify glycogen, glycogen phosphate, and chain length of glucose monomers. Simultaneous quantitation of glycogen architecture is challenging. This work describes a highly-sensitive method for the detection of both glycogen-derived glucose and glucose-phosphate esters utilizing gas-chromatography coupled mass spectrometry (GCMS). We then integrated this glycogen quantitation workflow into a routinely utilized metabolomics-based assay. The work presented describes a workflow for the normalization of polar metabolites utilizing amino acids derived from …

The Role Of Chromatin Structure In Circular Rna Biogenesis, Function And Disease, Rebekah Eleazer

Theses and Dissertations--Molecular and Cellular Biochemistry

Fundamental to cellular differentiation, development, and physiology is the precise regulation of gene expression. At the center of this control lies chromatin, and disruptions in chromatin biology are key contributors to complex human diseases. By convention, gene expression analysis primarily focuses on transcript abundance, however the splicing of transcripts into distinct isoforms is also of biological relevance. One new form of alternative splicing, backsplicing, is responsible for generating circular RNAs (circRNAs), which are emerging as essential components to gene regulatory networks. However, unlike other forms of alternative splicing, the role that chromatin plays in backsplicing and circRNA formation remains poorly …

Investigating The Roles Of Platelet Bioenergetics In Hemostasis And Thrombosis, Kanakanagavalli Shravani Prakhya

Theses and Dissertations--Molecular and Cellular Biochemistry

Platelets use a series of energy-dependent processes to mediate hemostasis, yet it is unclear which metabolic fuels/pathways are critical for platelet function. Platelets are metabolically flexible, switching between glycolysis and oxidative phosphorylation (OxPhos) depending on oxygen tension and substrate availability. The deletion of GLUT1 and GLUT3 together revealed an essential role in platelet function and hemostasis. To study platelet bioenergetics, we used clot contraction. We created a sensitive and inexpensive method to simultaneously assess clot contraction kinetics to directly compare and define the effects of different treatments or genetic manipulations on platelet function.

Since the pharmacological inhibitors cannot be used …

The Role Of Dynamin Related Protein 1-Mediated Mitochondrial Dynamics In Colorectal Cancer, Sumati Raj Hasani

Theses and Dissertations--Molecular and Cellular Biochemistry

Cancer cells are known for their ability to adapt variable metabolic programs depending on the availability of specific nutrients. Consequently, metabolic reprogramming has been increasingly recognized as a major mechanism that fuels tumorigenesis and disease progression. Here, we have investigated the role of a pro-fission factor, Dynamin-Related Protein 1 (Drp1), in promoting metabolic adaptation in colon cancer.

Our studies have shown that fatty acid (FAs) uptake alters cellular metabolic pathways in colon cancer cells to favor fatty acid oxidation through the activation of Drp1. Uptake of FAs induces mitochondrial fragmentation by promoting ERK-dependent phosphorylation of Drp1 at the S616 site, …

The Development And Characterization Of Nanobodies Specific To Protein Tyrosine Phosphatase 4a3 (Ptp4a3/Prl-3) To Dissect And Target Its Role In Cancer., Caroline Smith

Theses and Dissertations--Molecular and Cellular Biochemistry

Protein Tyrosine Phosphatase 4A3 (PTP4A3 or PRL-3) is an oncogenic dual-specificity phosphatase that drives tumor metastasis, promotes cancer cell survival, and is correlated with poor patient prognosis in a variety of solid tumors and leukemias. The mechanisms that drive PRL-3’s oncogenic functions are not well understood, in part due to a lack of research tools available to study this protein. The development of such tools has proven difficult, as the PRL family is ~80% homologous and the PRL catalytic binding pocket is shallow and hydrophobic. Currently available small molecules do not exhibit binding specificity for PRL-3 over PRL family members, …

Opportunities And Challenges In Augmenting Honey Bee Forage Resources With Pasture Legumes In Southern Australia, M. Kratz, M. Brown, M. T. Newell, R. C. Hayes

IGC Proceedings (1977-2023)

More than 80% of Australian honey is produced from native tree and shrub species. However, with increasing demands on public lands along with production risks posed by drought, floods and wildfires, there is a need to identify alternative forage resources to augment Australian honey production. With over 30 pasture legumes now available for agriculture in southern Australia, opportunity exists to increase the utilisation of some species with co-benefits to multiple production industries. However, there is little understanding of the potential value of most pasture legumes for honey production, and side by side comparisons are complicated by factors such as differences …

Examining The Effects Of Hyperglycemia On Retinal Regeneration, Emmanuella Ec Kyllians

Theses and Dissertations--Biology

Diabetes is a disease that affects how the body processes glucose, leading to a cascade of other complications, one of which is diabetic retinopathy (DR). DR is a neurovascular disease that affects the retina and research has revealed the role of retinal neurodegeneration in its development. Animal models like zebrafish have emerged as important tools for studying the molecular and cellular mechanisms underlying DR. On the other hand, retinitis pigmentosa (RP) is a degenerative disease that involves the progressive degeneration of rods and cones, and hyperglycemia has been found to impair regeneration. Therefore, this study aims to examine the effects …

Brain Glycogen – Beyond Energy Storage In Glycogen Storage Diseases, Kia H. Markussen

Theses and Dissertations--Molecular and Cellular Biochemistry

Glycogen is a carbohydrate molecule that is traditionally viewed as a convenient and easily accessible energy storage form of glucose. However,emerging evidence supports the role of glycogen as more than a glucose storage form. During the last 20 years, glycogen has been shown to play pivotal roles in learning and memory, signaling events, viscosity, protein glycosylation, and be acritical hallmark in devastating diseases. Not only, does glycogen play a role as an energy substrate and critical metabolite during energy deprivation, glycogen is central for neurotransmitter homeostasis, tumor initiation, and ontributes to proper protein glycosylation in the brain. In this work, …

Protein S In Coagulation And Inflammation, Martha Mega Silvia Sim

Theses and Dissertations--Molecular and Cellular Biochemistry

Protein S (PS) is a key regulator, which links inflammation and coagulation and performs multiple proposed functions in both processes. PS exists in the blood as a free soluble form (~40%), bound to complement component 4b-binding protein/ C4BP (~60%), and packaged in platelet α-granules (~2.5%). Subendothelial tissue factor (TF), upon exposure to blood, initiates coagulation, a proteolytic cascade which results in the activation of thrombin, the enzyme responsible for formation of a fibrin clot. PS is a critical anticoagulant that inhibits multiple steps of this process. Only the free fraction of PS has full anticoagulant properties, as C4BP blocks this …