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Body Mass Index Percentiles Versus Body Composition Assessments: Challenges For Disease Risk Classifications In Children, Jody L. Clasey, Elizabeth Ann Easley, Margaret O'Bryan Murphy, Stefan G. Kiessling, Arnold Stromberg, Aric Schadler, Hong Huang, John A. Bauer

UK CARES Faculty Publications

Background: Identifying at-risk children with optimal specificity and sensitivity to allow for the appropriate intervention strategies to be implemented is crucial to improving the health and well-being of children. We determined relationships of body mass indexes for age and sex percentile (BMI%) classifications to actual body composition using validated and convenient methodologies and compared fat and non-fat mass estimates to normative cut-off reference values to determine guideline reliability. We hypothesized that we would achieve an improved ability to identify at-risk children using simple, non-invasive body composition and index measures.

Methods: Cross-sectional study of a volunteer convenience sample of 1,064 (537 …

Existing Transportation Infrastructure In The Central Appalachian Basin To Support Core-Cm, Bryan Gibson, Robin Baskette, Doug Kreis

Kentucky Transportation Center Research Report

Stretching from Tennessee northeastward through southwestern Pennsylvania, the Central Appalachian Basin’s economic fortunes have long depended on coal production. However, as the U.S. has increasingly turned toward new forms of energy to generate electricity, the region’s economy has faced inexorable headwinds. Yet, what is clear is that the extractive industries are likely to play a key role in shaping the basin’s economy in the years to come. Taking this assumption as a starting point, this paper discusses how well current transportation infrastructure can support expanded us of the basin’s carbon ore, rare earth, and critical minerals (CORE-CM), other minerals, and …

Loss Of Peroxiredoxin Iv Protects Mice From Azoxymethane/Dextran Sulfate Sodium-Induced Colorectal Cancer Development, Pratik Thapa, Hong Jiang, Na Ding, Yanning Hao, Aziza Alshahrani, Eun Young Lee, Junichi Fujii, Qiou Wei

Markey Cancer Center Faculty Publications

Peroxiredoxin IV (Prx4), a typical two-cysteine-containing member of the peroxidase family, functions as an antioxidant to maintain cellular redox homeostasis through the reduction of reactive oxygen species (ROS) via cycles of oxidation–reduction reactions. Under oxidative stress, all Prxs including Prx4 are inactivated as their catalytic cysteines undergo hyperoxidation, and hyperoxidized two-cysteine Prxs can be exclusively repaired and revitalized through the reduction cycle catalyzed by sulfiredoxin (Srx). Previously, we showed that Prx4 is a preferred substrate of Srx, and knockout of Srx in mice leads to resistance to azoxymethane/dextran sulfate sodium (AOM/DSS)-induced colon carcinogenesis. To further understand the significance of the …

Naltrexone-Bupropion Combinations Do Not Affect Cocaine Self-Administration In Humans, Sean D. Regnier, William W. Stoops, Joshua A. Lile, Joseph L. Alcorn Iii, B. Levi Bolin, Anna R. Reynolds, Lon R. Hays, Abner O. Rayapati, Craig R. Rush

Behavioral Science Faculty Publications

The FDA has not yet approved a pharmacotherapy for cocaine use disorder despite nearly four decades of research. This study determined the initial efficacy, safety, and tolerability of naltrexone-bupropion combinations as a putative pharmacotherapy for cocaine use disorder.

Thirty-one (31) non-treatment seeking participants with cocaine use disorder completed a mixed-design human laboratory study. Participants were randomly assigned to the naltrexone conditions (i.e., 0, 50 mg/ day; between-subject factor) and maintained on escalating doses of bupropion (i.e., 0, 100, 200, 400 mg/day; within-subject factor) for at least four days prior to the conduct of experimental sessions. Cocaine self-administration (IN, 0, 40, …

Exposure To Pcb126 During The Nursing Period Reversibly Impacts Early-Life Glucose Tolerance, Brittany Breon Rice, Keegan W. Sammons, Sara Y. Ngo Tenlep, Madeline T. Weltzer, Leryn J. Reynolds, Cetewayo S. Rashid, Hollie I. Swanson, Kevin J. Pearson

Markey Cancer Center Faculty Publications

Polychlorinated biphenyls (PCBs) are persistent environmental organic pollutants known to have detrimental health effects. Using a mouse model, we previously demonstrated that PCB126 exposure before and during pregnancy and throughout the perinatal period adversely affected offspring glucose tolerance and/or body composition profiles. The purpose of this study was to investigate the glucose tolerance and body composition of offspring born to dams exposed to PCB126 during the nursing period only. Female ICR mice were bred, and half of the dams were exposed to either vehicle (safflower oil) or 1 μmole PCB126 per kg of body weight via oral gavage on postnatal …

Latexin Regulates Sex Dimorphism In Hematopoiesis Via Gender-Specific Differential Expression Of Microrna 98-3p And Thrombospondin 1, Xiaojing Cui, Cuiping Zhang, Fang Wang, Xinghui Zhao, Shuxia Wang, Jinpeng Liu, Daheng He, Chi Wang, Feng-Chun Yang, Sheng Tong, Ying Liang

Markey Cancer Center Faculty Publications

Hematopoietic stem cells (HSCs) have the ability to self-renew and differentiate to all blood cell types. HSCs and their differentiated progeny show sex/gender differences. The fundamental mechanisms remain largely unexplored. We previously reported that latexin (Lxn) deletion increased HSC survival and repopulation capacity in female mice. Here, we find no differences in HSC function and hematopoiesis in Lxn knockout (Lxn-/- male mice under physiologic and myelosuppressive conditions. We further find that Thbs1, a downstream target gene of Lxn in female HSCs, is repressed in male HSCs. Male-specific high expression of micro- RNA 98-3p (miR98-3p) contributes to Thbs1 suppression in male …

Chronic Environmental Circadian Disruption Increases Atherosclerosis And Dyslipidemia In Female, But Not Male, Apolipoproteine-Deficient Mice, Jeffrey M. Chalfant, Deborah A. Howatt, Victoria B. Johnson, Lisa R. Tannock, Alan Daugherty, Julie S. Pendergast

Saha Cardiovascular Research Center Faculty Publications

Shift work chronically disrupts circadian rhythms and increases the risk of developing cardiovascular disease. However, the mechanisms linking shift work and cardiovascular disease are largely unknown. The goal of this study was to investigate the effects of chronically shifting the light-dark (LD) cycle, which models the disordered exposure to light that may occur during shift work, on atherosclerosis. Atherosclerosis is the progressive accumulation of lipid-filled lesions within the artery wall and is the leading cause of cardiovascular disease. We studied ApolipoproteinE-deficient (ApoE−/−) mice that are a well-established model of atherosclerosis. Male and female ApoE−/− mice were housed in control 12L:12D …

Cervical Spinal Cord Injury Leads To Injury And Altered Metabolism In The Lungs, Emily E. Huffman, Brittany E. Dong, Harrison A. Clarke, Lyndsay E. A. Young, Matthew S. Gentry, Derek B. Allison, Ramon C. Sun, Christopher M. Waters, Warren J. Alilain

Saha Cardiovascular Research Center Faculty Publications

High-cervical spinal cord injury often disrupts respiratory motor pathways and disables breathing in the affected population. Moreover, cervically injured individuals are at risk for developing acute lung injury, which predicts substantial mortality rates. While the correlation between acute lung injury and spinal cord injury has been found in the clinical setting, the field lacks an animal model to interrogate the fundamental biology of this relationship. To begin to address this gap in knowledge, we performed an experimental cervical spinal cord injury (N = 18) alongside sham injury (N = 3) and naïve animals (N = 15) to assess lung injury …

Ferric Chloride-Induced Arterial Thrombosis And Sample Collection For 3d Electron Microscopy Analysis, Smita Joshi, Alexis N. Smith, Kanakanagavalli Shravani Prakhya, Hammodah Rawhi Hammodah Alfar, Joshua Lykins, Ming Zhang, Irina D. Pokrovskaya, Maria A. Aronova, Richard D. Leapman, Brian Storrie, Sidney W. Whiteheart

Saha Cardiovascular Research Center Faculty Publications

Cardiovascular diseases are a leading cause of mortality and morbidity worldwide. Aberrant thrombosis is a common feature of systemic conditions like diabetes and obesity, and chronic inflammatory diseases like atherosclerosis, cancer, and autoimmune diseases. Upon vascular injury, usually the coagulation system, platelets, and endothelium act in an orchestrated manner to prevent bleeding by forming a clot at the site of the injury. Abnormalities in this process lead to either excessive bleeding or uncontrolled thrombosis/insufficient antithrombotic activity, which translates into vessel occlusion and its sequelae. The FeCl3-induced carotid injury model is a valuable tool in probing how thrombosis initiates and progresses …

An Improved Germline Genome Assembly For The Sea Lamprey Petromyzon Marinus Illuminates The Evolution Of Germline-Specific Chromosomes, Nataliya Timoshevskaya, Kaan İ. Eşkut, Vladimir A. Timoshevskiy, Sofia M.C. Robb, Carson Holt, Jon E. Hess, Hugo J. Parker, Cindy F. Baker, Allison K. Miller, Cody Saraceno, Mark Yandell, Robb Krumlauf, Shawn R. Narum, Ralph T. Lampman, Neil J. Gemmell, Jacquelyn Mountcastle, Bettina Haase, Jennifer R. Balacco, Giulio Formenti, Sarah Pelan, Ying Sims, Kerstin Howe, Olivier Fedrigo, Erich D. Jarvis, Jeramiah James Smith

Markey Cancer Center Faculty Publications

Programmed DNA loss is a gene silencing mechanism that is employed by several vertebrate and nonvertebrate lineages, including all living jawless vertebrates and songbirds. Reconstructing the evolution of somatically eliminated (germline-specific) sequences in these species has proven challenging due to a high content of repeats and gene duplications in eliminated sequences and a corresponding lack of highly accurate and contiguous assemblies for these regions. Here, we present an improved assembly of the sea lamprey (Petromyzon marinus) genome that was generated using recently standardized methods that increase the contiguity and accuracy of vertebrate genome assemblies. This assembly resolves highly contiguous, somatically …

Chalcone Derivative Cx258 Suppresses Colorectal Cancer Via Inhibiting The Top2a/Wnt/Β-Catenin Signaling, Xi Chen, Xiaocheng Lv, Lijie Gao, Jiawei Liu, Wei Wang, Lichao Guo, Mykhaylo S. Frasinyuk, Wen Zhang, David S. Watt, Chunming Liu, Xifu Liu

Markey Cancer Center Faculty Publications

The deregulation in the Wnt/β-catenin signaling pathway is associated with many human cancers, particularly colorectal cancer (CRC) and, therefore, represents a promising target for drug development. We have screened over 300 semisynthetic and natural compounds using a Wnt reporter assay and identified a family of novel chalcone derivatives (CXs) that inhibited Wnt signaling and CRC cell proliferation. Among them, we selected CX258 for further in vitro and in vivo study to investigate the molecular mechanisms. We found that CX258 significantly inhibited β-catenin expression and nuclear translocation, inducing cell cycle arrest at the G2/M phase in CRC cells. Additionally, CX258 reduced …

Description Of Reaction And Vibrational Energetics Of Co2-Nh3interaction Using Quantum Computing Algorithms, Manh Tien Nguyen, Yueh-Lin Lee, Dominic Alfonso, Qing Shao, Yuhua Duan

Markey Cancer Center Faculty Publications

CO2 capture is critical to solving global warming. Amine-based solvents are extensively used to chemically absorb CO2. Thus, it is crucial to study the chemical absorption of CO2 by amine-based solvents to better understand and optimize CO2 capture processes. Here, we use quantum computing algorithms to quantify molecular vibrational energies and reaction pathways between CO2 and a simplified amine-based solvent model—NH3. Molecular vibrational properties are important to understanding kinetics of reactions. However, the molecule size correlates with the strength of anharmonicity effect on vibrational properties, which can be challenging to address using classical computing. Quantum computing can help enhance molecular …

Endogenous Glycoprotein Gpm6a Is Involved In Neurite Outgrowth In Rat Dorsal Root Ganglion Neurons, Gabriela I. Aparicio, Antonella León, Rocío Gutiérrez Fuster, Baylen Ravenscraft, Paula V. Monje, Camila Scorticati

Markey Cancer Center Faculty Publications

The peripheral nervous system (PNS) has a unique ability for self-repair. Dorsal root ganglion (DRG) neurons regulate the expression of different molecules, such as neurotrophins and their receptors, to promote axon regeneration after injury. However, the molecular players driving axonal regrowth need to be better defined. The membrane glycoprotein GPM6a has been described to contribute to neuronal development and structural plasticity in central-nervous-system neurons. Recent evidence indicates that GPM6a interacts with molecules from the PNS, although its role in DRG neurons remains unknown. Here, we characterized the expression of GPM6a in embryonic and adult DRGs by combining analysis of public …

Kegg_Pull: A Software Package For The Restful Access And Pulling From The Kyoto Encyclopedia Of Gene And Genomes, Erik D. Huckvale, Hunter N. B. Moseley

Markey Cancer Center Faculty Publications

Background: The Kyoto Encyclopedia of Genes and Genomes (KEGG) provides organized genomic, biomolecular, and metabolic information and knowledge that is reasonably current and highly useful for a wide range of analyses and modeling. KEGG follows the principles of data stewardship to be findable, accessible, interoperable, and reusable (FAIR) by providing RESTful access to their database entries via their web-accessible KEGG API. However, the overall FAIRness of KEGG is often limited by the library and software package support available in a given programming language. While R library support for KEGG is fairly strong, Python library support has been lacking. Moreover, there …

Understanding Livestock Grazing Impacts: A Decision Support Tool To Develop Goal-Oriented Grazing Management Strategies, S. J. Barry, K. Guenther, G. Hayes, R. Larson, G. Nader, M. Doran

IGC Proceedings (1977-2023)

Managing grasslands in the western United States has become much more complex over the last few decades. A century ago the goal was to survive as a livestock producer, and grassland management involved using forage effectively and overcoming obstacles such as predators and shortages of water and feed. Today the successful grassland manager also needs to consider the diversity and health of the ecosystem as a whole. Livestock grazing can negatively and/or positively affect riparian areas, sensitive plants, and endangered wildlife. Since the impact on a specific factor will vary depending on the timing, intensity and class of livestock grazed, …

Extending The Grazing Season With Turnips, P. Thomet, S. Kohler

IGC Proceedings (1977-2023)

A comparison of the actual feeding costs on 86 typical Swiss dairy farms confirmed that the production of hay and grass silage is very expensive with costs from the field to intake in the range of 20-25 Euro cents/kg of dry matter (DM). Options to extend the grazing season for dairy cows in the late autumn and early winter, and hence reduce winter feeding costs, would therefore be welcome (Penrose et al., 1996). The aim of the study was to compare the DM production potential of summer-seeded turnips with other brassicas and Italian ryegrass and to test whether dairy cows …

Recent Developments In Methods To Characterise The Chemical And Biological Parameters Of Grass Silage, R. S. Park, R. E. Agnew, M. G. Porter

IGC Proceedings (1977-2023)

Key points

1. Chemical analysis of forages is expensive, time consuming, environmentally unfriendly and relates poorly to the feed value for production purposes.
2. In vivo characterisation of animal feed is not a feasible option in terms of cost and analysis time.
3. NIRS is a rapid, non destructive, environmentally friendly, multi-analytical technique which can estimate the nutritive value of the feed.
4. NIRS predictive equations developed on a master instrument can be transferred to local and international sites.
5. Future assessment of forages necessitates rapid, stable, instrumentation for ‘in field’ studies.

Effects Of Two Different Chopping Lengths Of Maize Silage On Silage Quality And Dairy Performance, K. Mahlkow, J. Thaysen

IGC Proceedings (1977-2023)

Maize silage harvested at dough stage contains less fibre structure than grass silage. Due to higher proportions of maize silage in dairy rations and due to the fact that with longer chopping length of maize silage the contribution of fibre structure increases. The objective of this study was to assess the effect of two chopping lengths in the silage on animal performance with respect to feed intake, milk yields and ingredients as well as physiological effects of digestion.

Parameters Of Ensiled Maize With Biological And Chemical Additives, J. Grajewski, A. Potkański, K. Raczkowska-Werwińska, M. Twarużek, B. Miklaszewska

IGC Proceedings (1977-2023)

The amount of maize grown in Poland has increased rapidly. Nowadays it takes about 600,000 ha, 40% of which is used as silage. Changing climate in Poland, with dry summer followed by wet autumn with ground frost causes extensive moulds contamination and high presence of the fusarium toxins in the maize during the harvest. The norms accepted in the EU concerning the acceptable level of deoxynivalenol (DON) and zearalenol (ZON) in feedstuffs for cattle require detailed examination of this problem as it decides on the health quality and production results. The aim of the study was to evaluate the effects …

Conjugated Linoleic Acid Content Of Milk From Cows Fed Different Diets, E. Staszak, J. Mikołajczak

IGC Proceedings (1977-2023)

Conjugated linoleic acid (CLA) is a mixture of positional and geometric isomers of linoleic acid (c-9, c-12 C_18:2). Conjugated linoleic acid occurs naturally in foods, however the main dietary sources are dairy products and other foods derived from ruminants. Continuous interest in CLA is attributed to its potential health benefits such as anticarcinogenic, antiatherogenic, antidiabetic and antiadipogenic effects (Dhiman et al., 1999, Staszak et al., 2001). Typical consumption of CLA by humans is far lower than the dose that has been shown to be effective in reducing tumours in animal models (Dhiman et al., 1999), …

Silage Production From Tropical Forages, Luiz Gustavo Nussio

IGC Proceedings (1977-2023)

Key Points

1. The determination of overall DM recovery is important in tropical grass silage systems
2. Silage fermentation profile and aerobic stability: trends and additional effects
3. Combinations of chemical and microbial additives might be useful for control of losses
4. Fermentation products should be considered in order to better predict animal performance
5. A critical points database would be a helpful management tool for the development of a set of HACCP principles for the production and utilisation of tropical grass silages

Update On Technologies For Producing And Feeding Silage, P. D. Forristal, P. O'Keily

IGC Proceedings (1977-2023)

Key Points

1. Mechanisation and engineering inputs are key factors which contribute to silage costs.
2. Sensing technologies will improve management precision in many areas of ensilage.
3. While current harvesting machines have high output capacity reflecting mature design, there is a need to revisit the area of energy efficiency.
4. Baled silage technology, particularly in the areas of covering film and wrapping technology, needs further research.
5. Feeding systems are well researched but there is a need for systems research to underpin farmers’ decisions concerning housing design and feeding system.

Research Into The Types Of Cows And Systems Required To Utilise Grazed Pastures Sustainably In 100 Years From Now, C. Holmes

IGC Proceedings (1977-2023)

Dairy farming has undergone rapid intensification in many countries over the past 60 years, as the result of technological developments, trade policies, and financial incentives. For example, the use of soluble fertilisers, irrigation and concentrated feeds have increased enormously, and antibiotics and hormone treatments did not even exist in 1940. However, the dramatic increases in milk yields per cow (e.g. in North America) and per hectare (e.g. in New Zealand) and in cows per person (in most countries) have been associated with growing concerns about the health and fertility of cows, and their metabolic stress and welfare and about the …

Recent Developments In Feeding Beef Cattle On Grass Silage-Based Diets, M. Mcgee

IGC Proceedings (1977-2023)

Key points:

1. High digestibility grass silage with moderate concentrate supplementation can sustain a large proportion of the cattle performance achieved on high concentrate diets.
2. Increasing concentrate supplementation reduces the importance of grass silage nutritional value.
3. Subsequent compensatory growth diminishes the advantage of concentrate supplementation of young cattle.
4. Meat quality and fatty acid composition can be influenced by grass silage-based diets.

Effects Of Species, Maturity And Additive On The Feed Quality Of Whole Crop Cereal Silage, E. Nadeau

IGC Proceedings (1977-2023)

Chemical composition of whole crop cereals differ among species and maturity stages. These chemical differences create variations in silage quality (Bergen et al., 1991). There is only limited information available on the effects of plant species and maturity on the use of additives for whole crop cereal silage. The objective of this experiment was to determine the effects of species, maturity, additive and their interactions on nutrient composition and fermentation characteristics of whole crop cereal silage.

Survey Of Tetraploid And Diploid Perennial Pastures In The Waikato For Number Of Spores Produced By The Fungus Pithomyces Chartarum, J. P. J. Eerens, W. W. Nichol, J. Waller, J. M. Mellsop, M. R. Trolove, M. G. Norriss

IGC Proceedings (1977-2023)

Facial eczema (FE) is a disease of livestock, caused by a toxin released into the bloodstream after digestion of spores of Pithomyces chartarum, a fungus residing in necrotic plant material in the base of pastures (di Menna & Bailey, 1973). Spore numbers tend to be highest in warm, humid conditions, where high post grazing residuals have lead to a build up of necrotic plant material. Tetraploid perennial ryegrass pastures tend to be more palatable, and with lower post gazing residuals, than equivalent diploid pastures; thus we hypothesised that spore numbers would be lower in tetraploid pastures. A survey of …

Correlation Between Epiphytic Microflora And Microbial Pollution And Fermentation Quality Of Silage Made From Grasses, B. Osmane, J. Blūzmanis

IGC Proceedings (1977-2023)

Grass forage in Latvia is the main and inexpensive cow feed, however its composition and nutritive value differ during the growth period of grasses. The traits of grasses, their natural ensilage capacity, count of epiphytic microflora, the timing of harvest and ensilage making technology affecting the quality of grass silage are important issues to be studied. Silage making for the winter period is the treatment of green material to minimise the breakdown of nutrients being the results of biochemical and microbiological processes. The aim of the research was to clarify the methodologies to reduce the count of epiphytic microflora and …

Perennial Ryegrass Variety Differences In Nutritive Value Characteristics, T. J. Gilliland, R. E. Agnew, A. M. Fearson, F. E. A. Wilson

IGC Proceedings (1977-2023)

Animal grazing performance at grass is predominately determined by herbage intake rates, with high yielding dairy cows requiring up to 20 kg/d DM within a limited grazing time (Gibb, 1998). Grass nutritional factors such as seasonal patterns in digestibility and water-soluble carbohydrate levels have been linked to animal productivity (Davies et al., 1991), while sward surface height, herbage mass, bulk density and green leaf mass have been shown to promote high grazing intake (Barrett et al., 2001). Furthermore, fatty acid profiles have been shown to improve the unsaturated fatty acid composition of milk, with potential human health benefits …

Optimising Financial Return From Grazing In Temperate Pastures, P. Dillon, J. R. Roche, L. Shalloo, B. Horan

IGC Proceedings (1977-2023)

Key Points

1. The increased interest in pasture-based systems of milk production in recent years has been largely generated through lower product prices and rising costs of production.

2. Pasture based systems of milk production decrease unit production costs, through lower feed and labour expenses, and reduced capital investment.

3. Systems utilising grazed pasture will be optimised in regions where pasture production potential is high, variability in seasonal pasture supply and quality is low, manufacturing milk accounts for a large proportion of total production, and where large areas of land are available at relatively low cost.

4. Pasture based systems may allow greater global …

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Challenges And Opportunities For Animal Production From Temperate Pastures, D. A. Clark

IGC Proceedings (1977-2023)

Key points

1. Temperate pastures offer a major opportunity to reduce the feed costs associated with ruminant production.

2. Pastures offer unique opportunities for producing high value components in feedstuffs that are beneficial to human health.

3. The increased use of pasture will not automatically lead to improved environmental outcomes – difficult challenges exist in reducing nitrogen and greenhouse gas pollution.

4. Grazed pasture systems offer advantages in animal welfare, provided management avoids the problems associated with climatic extremes, and the toxins associated with some pastures.

5. To remain competitive with total mixed ration systems, and environmentally acceptable, pastures with higher intake characteristics that allow …

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