Digital Commons Network^™

Deficiency Of Acute-Phase Serum Amyloid A Exacerbates Sepsis-Induced Mortality And Lung Injury In Mice, Ailing Ji, Andrea C. Trumbauer, Victoria P. Noffsinger, Luke W. Meredith, Brittany Dong, Qian Wang, Ling Guo, Xiangan Li, Frederick C. De Beer, Nancy R. Webb, Lisa R. Tannock, Marlene E. Starr, Christopher M. Waters, Preetha Shridas

Saha Cardiovascular Research Center Faculty Publications

Serum amyloid A (SAA) is a family of proteins, the plasma levels of which may increase >1000-fold in acute inflammatory states. We investigated the role of SAA in sepsis using mice deficient in all three acute-phase SAA isoforms (SAA-TKO). SAA deficiency significantly increased mortality rates in the three experimental sepsis mouse models: cecal ligation and puncture (CLP), cecal slurry (CS) injection, and lipopolysaccharide (LPS) treatments. SAA-TKO mice had exacerbated lung pathology compared to wild-type (WT) mice after CLP. A bulk RNA sequencing performed on lung tissues excised 24 h after CLP indicated significant enrichment in the expression of genes associated …

Ecohiv-Infected Mice Show No Signs Of Platelet Activation, Hammodah Rawhi Hammodah Alfar, Dominic Ngima Nthenge-Ngumbau, Kathryn E. Saatman, Sidney W. Whiteheart

Saha Cardiovascular Research Center Faculty Publications

Platelets express several surface receptors that could interact with different viruses. To understand the mechanisms of HIV-1′s interaction with platelets, we chose the EcoHIV model. While EcoHIV is an established model for neuroAIDS, its effects on platelets are ill-defined. Our results indicate that EcoHIV behaves differently from HIV-1 and is cleared from circulation after 48 h post- infection. The EcoHIV course of infection resembles an HIV-1 infection under the effects of combined antiretroviral therapy (cART) since infected mice stayed immunocompetent and the virus was readily detected in the spleen. EcoHIV-infected mice failed to become thrombocytopenic and showed no signs of …

Loss Of Carnitine Palmitoyltransferase 1a Reduces Docosahexaenoic Acid-Containing Phospholipids And Drives Sexually Dimorphic Liver Disease In Mice, Mikala M. Zelows, Corissa Cady, Nikitha Dharanipragada, Anna E. Mead, Zachary A. Kipp, Evelyn A. Bates, Venkateshwari Varadharajan, Rakhee Banerjee, Se-Hyung Park, Nathan R. Shelman, Harrison A. Clarke, Tara R. Hawkinson, Terrymar Medina, Ramon C. Sun, Todd A. Lydic, Terry D. Hinds, Jr., J. Mark Brown, Samir Softic, Gregory A. Graf, Robert N. Helsley

Saha Cardiovascular Research Center Faculty Publications

Background and aims: Genome and epigenome wide association studies identified variants in carnitine palmitoyltransferase 1a (CPT1a) that associate with lipid traits. The goal of this study was to determine the role of liver-specific CPT1a on hepatic lipid metabolism.

Approach and results: Male and female liver-specific knockout (LKO) and littermate controls were placed on a low-fat or high-fat diet (60% kcal fat) for 15 weeks. Mice were necropsied after a 16 h fast, and tissues were collected for lipidomics, matrix-assisted laser desorption ionization mass spectrometry imaging, kinome analysis, RNA-sequencing, and protein expression by immunoblotting. Female LKO mice had increased serum alanine …

On The Origin And Evolution Of The Mosquito Male-Determining Factor Nix, James K. Biedler, Azedah Aryan, Yumin Qi, Aihua Wang, Ellen O. Martinson, Daniel A. Hartman, Fan Yang, Atashi Sharma, Katherine S. Morton, Mark Potters, Chujia Chen, Stephen L. Dobson, Gregory D. Ebel, Rebekah C. Kadling, Sally Paulson, Rui-De Xue, Michael R. Strand, Zhijian Tu

Entomology Faculty Publications

The mosquito family Culicidae is divided into 2 subfamilies named the Culicinae and Anophelinae. Nix, the dominant male-determining factor, has only been found in the culicines Aedes aegypti and Aedes albopictus, 2 important arboviral vectors that belong to the subgenus Stegomyia. Here we performed sex-specific whole-genome sequencing and RNAseq of divergent mosquito species and explored additional male-inclusive datasets to investigate the distribution of Nix. Except for the Culex genus, Nix homologs were found in all species surveyed from the Culicinae subfamily, including 12 additional species from 3 highly divergent tribes comprising 4 genera, suggesting Nix originated at least 133 to …

Cyclin D1 Extensively Reprograms Metabolism To Support Biosynthetic Pathways In Hepatocytes, Heng Wu, Betsy T. Kren, Andrew N. Lane, Teresa A. Cassel, Richard M. Higashi, Teresa Whei-Mei Fan, George S. Scaria, Laurie L. Shekels, Mark A. Klein, Jeffrey H. Albrecht

Markey Cancer Center Faculty Publications

Cell proliferation requires metabolic reprogramming to accommodate biosynthesis of new cell components, and similar alterations occur in cancer cells. However, the mechanisms linking the cell cycle machinery to metabolism are not well defined. Cyclin D1, along with its main partner cyclindependent kinase 4 (Cdk4), is a pivotal cell cycle regulator and driver oncogene that is overexpressed in many cancers. Here, we examine hepatocyte proliferation to define novel effects of cyclin D1 on biosynthetic metabolism. Metabolomic studies reveal that cyclin D1 broadly promotes biosynthetic pathways including glycolysis, the pentose phosphate pathway, and the purine and pyrimidine nucleotide synthesis in hepatocytes. Proteomic …

Immunization With The Amino-Terminus Region Of Dense Granule Protein 6 (Gra6) Of Toxoplasma Gondii Activates Cd8+ Cytotoxic T Cells Capable Of Removing Tissue Cysts Of The Parasite Through Antigen Presentation By Human Hla-A2.1, Rajesh Mani, Corinne Mercier, Marie-France Delauw, Yasuhiro Suzuki

Markey Cancer Center Faculty Publications

CD8þ T cells from HLA-A2.1-transgenic mice, but not wild-type mice, immunized with the amino- terminus region (aa 41e152) of dense granule protein 6 (GRA6Nt) of Toxoplasma gondii secreted large amounts of perforin and granzyme B in response to GRA6Nt through antigen presentation by HLA-A2.1 in vitro. When those CD8þ T cells were transferred into chronically infected HLA-A2.1-expressing NSG mice deficient in T cells, cerebral cyst burden of the recipients of HLA-A2.1-transgenic T cells, but not of WT T cells, became significantly less than that of control mice with no cell transfer. Furthermore, the significant reduction of the cyst burden by …

Magnetic Resonance Imaging Of Macrophage Response To Radiation Therapy, Harrison Yang, Brock Howerton, Logan Brown, Tadahide Izumi, Dennis C. Cheek, Jason Anthony Brandon, Francesc Marti, Roberto Gedaly, Reuben Adatorwovor, Fanny Chapelin

Markey Cancer Center Faculty Publications

Background: Magnetic resonance imaging (MRI) is a non-invasive imaging modality which, in conjunction with biopsies, provide a qualitative assessment of tumor response to treatment. Intravenous injection of contrast agents such as fluorine (19F) nanoemulsions labels systemic macrophages, which can, then, be tracked in real time with MRI. This method can provide quantifiable insights into the behavior of tumor-associated macrophages (TAMs) in the tumor microenvironment and macrophage recruitment during therapy.

Methods: Female mice received mammary fat pad injections of murine breast or colon cancer cell lines. The mice then received an intravenous 19F nanoemulsion injection, followed by a baseline 19F MRI. …

Md_Harmonize: A Python Package For Atom-Level Harmonization Of Public Metabolic Databases, Huan Jin, Hunter N. B. Moseley

Markey Cancer Center Faculty Publications

A major challenge to integrating public metabolic resources is the use of different nomenclatures by individual databases. This paper presents md_harmonize, an open-source Python package for harmonizing compounds and metabolic reactions across various metabolic databases. The md_harmonize package utilizes a neighborhood-specific graph coloring method for generating a unique identifier for each compound via atom identifiers based on a compound’s chemical structure. The resulting harmonized compounds and reactions can be used for various downstream analyses, including the construction of atom-resolved metabolic networks and models for metabolic flux analysis. Parts of the md_harmonize package have been optimized using a variety of computational …

Design And Characterization Of A Novel Eef2k Degrader With Potent Therapeutic Efficacy Against Triple-Negative Breast Cancer, Changxin Zhong, Rongfeng Zhu, Ting Jiang, Sheng Tian, Xiaobao Zhao, Xiaoya Wan, Shilong Jiang, Zonglin Chen, Rong Gong, Linhao He, Jin-Ming Yang, Na Ye, Yan Cheng

Markey Cancer Center Faculty Publications

Dysregulated eEF2K expression is implicated in the pathogenesis of many human cancers, including triple-negative breast cancer (TNBC), making it a plausible therapeutic target. However, specific eEF2K inhibitors with potent anti-cancer activity have not been available so far. Targeted protein degradation has emerged as a new strategy for drug discovery. In this study, a novel small molecule chemical is designed and synthesized, named as compound C1, which shows potent activity in degrading eEF2K. C1 selectively binds to F8, L10, R144, C146, E229, and Y236 of the eEF2K protein and promotes its proteasomal degradation by increasing the interaction between eEF2K and the …

Growth Components And Yield Of Brachiaria Ruziziensis (Congo Grass) And Centrosema Pascuorum (Centro) Mixtures As Influenced By Mixture Ratios, Phosphorus Levels And Stage Of Harvest, I. Sani, J. T. Amodu, R. J. Tanko, M. R. Hassan, N. Adamu

IGC Proceedings (1977-2023)

This study was conducted during the 2017 and 2018 rainy season at the Crop Production Research farm, Abubakar Tafawa Balewa University, Bauchi to determine the effects of plant mixture ratios in Brachiaria ruziziensis and Centrosema pascuorum (100% Sole B. ruziziensis, 75% B.R: 25% C.P, 50% B.R: 50% C.P, 25% B.R: 75% C.P and 100% Sole C. pascuorum) and phosphorus fertilizer (SSP) levels (0, 100 and 150kg/ha). The experiment was in a split-plot design with 5x3 factorial arrangement in which the mixture ratios constituted the main plots while phosphorus (SSP) levels constituted the sub treatments. Number of plants per …

Carbon-Nitrogen Ratios In Rangeland Soils In Various Agriculture Response Units In Three Watersheds In The Central Himalayas, India, Nanda Nautiyal, Vir Singh

IGC Proceedings (1977-2023)

CN ratios of rangeland soils in selected agriculture response units (ARUs) prevailing in three watersheds, Kosi, Alaknanda, and Pindar, in the Indian Central Himalayan mountains were analyzed. The ratios varied significantly (P

Impact Of The Brachiaria Hybrids On Both Soil Health And Carbon Stock On Livestock Production, A. Silva, T. Madeira, D. Florian, E. A. Pizarro, R. N. Jauregui, G. Lobato, A. Santi, D. Fagundes, F. Pinheiro, A. Rieger, A. Cagliari

IGC Proceedings (1977-2023)

Pastures occupy 30% of Earth and 80% of the entire agricultural area of the planet. To ensure food to the world and contribute to the quality of the environment, pasture-based animal production systems will also have to undergo through a more intense evolution. The intensification of tropical grasslands is an important strategy of land utilization in developing countries, contributing to increase production and minimize environmental impact through the best management practices. In this sense, the use of Brachiaria hybrids represents an excellent option, since combining the best traits of different Brachiaria species, with higher nutritive value, forage, and seed yield. …

Evaluating Functional Diversity As Potential Early-Warning Indicator Of Rangeland Degradation, L.-M. Schwarz, C. P. Carmona, M. C. Bilton, F. Munyebvu-Chambara, K. Behn, A. Linstädter

IGC Proceedings (1977-2023)

Droughts and overgrazing play a crucial role in the degradation of semi-arid rangelands. This is evident in the loss of palatable long-lived grass species and bush encroachment. Early warning indicators are needed to mitigate long-term degradation and decline in essential forage provision. Functional diversity provides valuable information on ecosystem health. However, functional diversity indices have not yet been tested regarding their applicability as early warning indicators, revealing non-linear threshold behaviour. We therefore examined the following questions: (1) How do functional diversity indices respond to grazing pressure? (2) Does land tenure affect the relationship between functional diversity and grazing pressure? (3) …

Enhancing Milk Production Of Lactating Camels In Kenya Via Supplementation Of The Invasive Cactus (Opuntia Stricta) In The Diet, W. O. Owino, Lucy W. Ikanya, J. G. Maina, C. K. Gachuiri, José C. B. Dubeux Jr.

IGC Proceedings (1977-2023)

Climate change leading to prolonged and recurrent droughts, changes in land use, primarily settlement of pastoralists, followed by overgrazing and subsequent land degradation, has made the highly drought resistant opuntia flourish and be aggressively invasive in the Kenya’s rangelands. Camel keeping has increasingly replaced cattle as a climate adaptation strategy and also as a result of a steady increase in demand for camel milk due to the associated nutritional and health benefits,To address the challenge of lack of pasture during prolonged drought, there is the need to utilize the invasive cactus as fodder. The invasive cactus can be a kind …

Potential Of Indigenous Desert Grasses For Forage Production In A Water-Scarce Region, A. E. Osman

IGC Proceedings (1977-2023)

Indigenous perennial grasses are widely distributed in the Arabian Peninsula. Their survival under limited rainfall and grazing suggests a potential role as grassland species and for rehabilitation of degraded rangelands. Forage productivity and water-use efficiency (WUE) was determined over 2 years for four indigenous grasses: buffel grass (Cenchrus ciliaris L.), dakhna (Coelachyrum piercei Benth.), da’ay (Lasiurus scindicus Henr.) and tuman (Panicum turgidum Forssk.) together with one exotic species, rhodes grass (Chloris gayana Kunth) in the central region of the United Arab Emirates. Three irrigation treatments were used: R1 (1858–6758 m³ ha^-1 year^{-1 …}

Natural Regeneration Of Severely Degraded Terrestrial Arid Ecosystems Needs More Than Just Removing The Cause Of The Degradation, M. Louhaichi, M. Gamoun, N. Al Hashash, F. Al-Ameer, D. Alrashedi, A. Redha, F. Alkandari, A. Niane

IGC Proceedings (1977-2023)

Rangelands cover over 75% of Kuwait’s total land area. Most of these rangelands are severely degraded because of overgrazing, poor anthropic utilization, and mismanagement. Restoring natural rangelands is a way to increase forage productivity, enhance biodiversity, and achieve sustainable development. When degradation has not reached the point of irreversibility, natural restoration through resting is one of the best low-cost restoration techniques. This study evaluated the effect of natural restoration on vegetation cover and species richness in the desert rangelands of Kuwait. The studied rangeland was a completely fenced area of 1 km2. The percent of vegetation was measured using the …

Back To The Future: Achieving Resilient, Sustainable Grasslands Through Restoration Of Ecological Norms / Overview – The Problem, The Opportunity, Pat D. Keyser, C. Bitler

IGC Proceedings (1977-2023)

Grasslands are one of the largest biomes on earth, are the largest agricultural enterprise in the U.S., serve as a major carbon pool, produce high quality dietary proteins, and host a rich biodiversity. However, grasslands, including those of the eastern U.S., have been heavily altered and degraded reducing their resiliency and negatively impacting native fauna. Recent scientific advances in grassland establishment and management, along with the opportunity to monetize the carbon benefits of native grasslands, could provide the needed social and economic support to make such restoration possible on a large scale. Where restored grassland communities align with historical, ecological …

Grass Vegetation Dynamics In Wetlands With Different Utilization, Dorai Pandian Kannan

IGC Proceedings (1977-2023)

The land-use patterns, combined with the biotic interactions, are likely to substantially affect the grass community of tropical wetlands. However, we need more information to understand the vegetation cover and, particularly, the diversity of grasses in the protected sites, where the naturally occurring interactions with extreme complexity. Hence a thorough understanding of these interactive effects on grass dynamics would help develop wildlife management guidelines to predict the grass-woody herbs and other associated vegetation. In light of this, a beta vegetation survey and diversity analysis in two varying wetland ecosystems of natural mountain streams and temporary ponds. Grass communities occurred along …

Enclosure Rather Than Topography Enhances The Soil Ecological Stoichiometry In Typical Steppe On The Loess Plateau, China, Zhaoxia Guo, Fujiang Hou, Jiao Ning

IGC Proceedings (1977-2023)

Grassland is one of the largest terrestrial ecosystems in the world, a large part of which is distributed in varied topography. And grazing and enclosure are the main ways to use this part. Grazing changes the soil structure through feeding, trampling and excreta return, thus affects the soil nutrients. The aspect mainly affects soil temperature and moisture by affecting solar radiation. The slope affects soil nutrients by affecting surface runoff. Water and temperature are the main factors affecting soil nutrients. We carried out to explore the effect of enclosure years and topography on soil ecological stoichiometry. The results showed that: …

Evaluation Of Restoration Of Damaged Ecosystems On Soil Nematode Communities And Their Functions In The Desert Steppe Open-Pit Mining Area Of Inner Mongolia, Zhiwei W. Gao, G. D. Han, G. G. Zhang

IGC Proceedings (1977-2023)

In view of the general lack of analysis of the ecological restoration effects of underground soil in the desert grassland ecological restoration projects in Inner Mongolia, this study selected the most representative open-pit quarries, open-pit iron mines and open-pit coal mines in the desert steppe. Using high-throughput sequencing technology, nematode community composition, diversity, and function were analyzed to determine the response of nematodes to different ecological restoration methods in damaged areas of coal mines. In open-pit coal mines, for slope rehabilitation, vegetation blanket restoration exhibited more favorable effects than those exhibited by vegetation bag restoration and natural restoration. For rehabilitation …

Surface-Soil Aggregation And Organic C And N Fractions Under Paired Grassland And Cropland Sites In The Southeastern Usa, Alan J. Franzluebbers, S. Van Vliet, M. H. Poore

IGC Proceedings (1977-2023)

Grasslands are expected to deliver ecosystem services by sequestering soil organic C, improving soil health and water quality, and minimizing soil erosion. Data to support these ecosystem services from contemporary managed grasslands are relatively scant, and so on-farm measurements would help bolster assessment across more diverse environmental settings. This study was conducted to compare soil properties from paired landuse of croplands and grasslands in a diversity of Major Land Resource Areas – the Piedmont, Blue Ridge, and Blackland Prairie of the southeastern USA. Four separate pastures on three collaborating farms were sampled at depth of 0-10 cm. Four samples from …

Forage Response And Economic Benefits To Weed Management In Grasslands, E. S. Flynn, S. Ingram, B. B. Sleugh

IGC Proceedings (1977-2023)

A common question among managers of grazing operations is “At what level of weed pressure does it become economical to apply herbicides on pastures?” Unfortunately, there isn’t just one answer to this question as production goals and practices differ between operations and even within an operation over time. Regardless, the real question being ask is if weed control will increase profit per acre.

There are three basic avenues that may be taken to improve profit per acre through weed control. The first is to increase the carrying capacity of the grazing operation by controlling weeds and replacing them with desirable …

Detection Of Endophyte Mycotoxins By Service Laboratories: Providing Answers For Safe Feed, J. Honneffer, M. Sulyok, V. Milojevic, A. Holman, Jennifer Duringer

IGC Proceedings (1977-2023)

. A global network of service laboratories exists to test livestock feed materials (typically grass hay and pellets) for ergovaline, ergot alkaloids and lolitrem B to ensure ‘safe feeds’ are being given to livestock. These compounds are mycotoxins produced by endophytic fungi that naturally reside in feed material. They have been purposely bred into grass species, as they enhance the plant’s survival from drought and insect predation. Unfortunately, ergovaline and other ergot alkaloids also cause vasoconstrictive effects and reproductive difficulties in livestock, resulting in a $1 million annual loss in production for the cattle industry in the USA alone. Lolitrem …

Sustainable Intensification Of Livestock Systems Using Forage Legumes In The Anthropocene, José C. B. Dubeux Jr., D. M. Jaramillo, E. R. S. Santos, L. Garcia, L. M. D. Queiroz, K. R. Trumpp

IGC Proceedings (1977-2023)

Sustainable intensification of livestock systems implies greater efficiency in resource utilization resulting in greater output of products and other ecosystem services per unit of resource input. Strategies to improve resource use efficiency include diversification of plant and ruminant species with complementary resource use. Forages that have root systems with contrasting architecture and exploring different soil layers with complementary use of resource acquisition (e.g., nutrients, water) could enhance primary productivity. Belowground interactions with soil microbiota (e.g., mycorrhizae) is key to enhance resource utilization. Forages with complementary canopy characteristics that helps enhancing light interception and utilization could also lead to greater resource …

Grazing Cover Crops For Soil Health In An Integrated Crop-Livestock System, Forrest E. Davis, J. Brett Rushing

IGC Proceedings (1977-2023)

. Optimizing soil health requires building an environment that creates conditions which allow for best function of the chemical, biological, and physical properties of the soil to thrive as an organism and an ecosystem. Integrated crop-livestock systems (ICLS) use a systems approach to provide improvements to soil health parameters. In the fall of 2021, a randomized complete block study was conducted at the Coastal Plain Branch Experiment Station (CPBES) in Newton, Mississippi and at the Prairie Research Unit (PRU) in Prairie, Mississippi to observe the soil health effects of grazing cereal rye cover crops in a soybean production system. Three …

Eragrostis Curvula Effects On Above And Below-Ground Plant Species Richness And Diversity, Jed Brown, A. Merchant, L. Ingram

IGC Proceedings (1977-2023)

Monitoring and managing the soil seed bank is fundamental to land management as it constitutes the future generations of invasive plant communities. Invasive plants have traits that result in high recruitment through increased seed generation, short seed dormancy and phenotypic plasticity. Furthermore, invasive plants with growth forms that inhibit the growth and recruitment of other species can lead to monocultures and associated reduction in above-ground biodiversity, potentially negatively impacting the soil seed bank diversity and ecosystem functions and services. Eragrostis curvula is one such species that has many of these invasive traits, including high propagule generation, and can exclude plant …

The Importance Of Forage Legume Epigenetics In The Anthropocene, J. Brady, J. P. Muir, A. Falk

IGC Proceedings (1977-2023)

As climates change and economic as well as ecological pressures to produce more ruminant product on less land in the Anthropocene increase, the importance of using cutting-edge methods for forage legume improvement grows. The immediate return rate and turnaround on investment is likely greater for multi-species incorporation into grasslands but focusing on intraspecific variability and diversity could also contribute. The role of forage legumes in cultivated pasture and rangeland biodiversity as well as stability depends on genetics as well as management. Their epigenetics, however, may become increasingly important as climatic extremes and ecological pressures grow due to human-induced factors. Unfortunately, …

Rangeland Health Status And Condition Two Different Yet Complementary Concepts: National Reserve Pampa Galeras Barbara D´Achille Case, R. Zárate, E. R. Flores

IGC Proceedings (1977-2023)

Pampa Galeras Barbara D´Achille National Reserve has an extension of 21,030 hectares constituted mainly by puna rangeland and has played a key role in the recovery of the vicuña (Vicugna vicugna) population from extinction. This reserve is divided into two zones, known as the Rigid zone with a total area of 6,500 ha, and the Buffer zone of 14,530 ha. The present study aimed to implement an Ecological Response Unit system (ERU) to create an evaluation and monitoring system of rangeland health and condition, that could serve as a model for the rest of the Protected National Areas …

Responses Of Plant Functional Diversity To Long-Term Warming And Nitrogen Addition In A Desert Steppe, Y. Zhu, L. Tian, H. Y. Ren, G. D. Han

IGC Proceedings (1977-2023)

Plant functional traits are closely related to ecosystem functions, and can indicate changes in ecosystem functions. Plant functional diversity is an important component of biodiversity, which can more accurately predict the changes of ecosystem functions or processes than species diversity, and is the main determinant of ecosystem functions or processes. In order to study the impact of global climate change factors (i.e., warming and nitrogen deposition) on the plant functional diversity in a desert steppe, we conducted a long-term warming and nitrogen addition experiment in northern China for 15 years (2006 - 2020). In 2020, we measured the plant height, …

Opening Speech, Ray Smith

IGC Proceedings (1977-2023)

https://youtu.be/CbRcQzf5z0o?si=ESOHxXF-jFsYMw2A