Digital Commons Network^™

Parental Perceptions Of Nutritional Status Of Children With Autism, Rachel Corliss Holt

University of Kentucky Master's Theses

Literature shows that nutritional deficiency and feeding behaviors in neurodevelopmentally disabled children is secondary to an underdeveloped gastrointestinal tract. Fifty (50) parents with children with autism responded to a Nutritional Perception Assessment that they believe their children are at adequate nutritional status. They reported confidence in their nutritional knowledge, yet admitted to concern about the nutritional status of their children. The survey revealed that parents are not seeing a registered dietitian for their nutritional advice, leading to the question of soundness in nutritional information received. A marginal correlation was found between belief in adequate nutritional status of children and the …

Aging And Sleep Stage Effects On Entropy Of Electroencephalogram Signals, Swetha Vennelaganti

University of Kentucky Master's Theses

The aging brain is characterized by alteration in synaptic contacts, which leads to decline of motor and cognitive functions. These changes are reflected in the age related shifts in power spectrum of electroencephalogram (EEG) signals in both wakefulness and sleep. Various non-linear measures have been used to obtain more insights from EEG analysis compared to the conventional spectral analysis. In our study we used Sample Entropy to quantify regularity of the EEG signal. Because elderly subjects arouse from sleep more often than younger subjects, we hypothesized that Entropy of EEG signals from elderly subjects would be higher than that from …

Assessing The Demand For Weather Index Insurance In Shandong Province, China, Lisha Zhang

University of Kentucky Master's Theses

Shandong Province, renowned as China’s greatest agricultural province, is dominated by smallholders growing rain-fed crops and vulnerable to severe weather shocks that can increase poverty rates. Weather index insurance, an innovative agricultural risk management product, may be an effective mechanism to address vulnerability to catastrophic weather risk in rural regions of China, including Shandong. This project evaluated current household livelihood and risk management strategies and farmer interest in weather index insurance. Data from 174 participants were collected using a methodology that included focus groups, questionnaires, and personal interviews. Despite limited access to formal financial services, Shandong farmers generally employ informal, …

Microbial Community Structure Dynamics In Ohio River Sediments During Reductive Dechlorination Of Pcbs, Andres Enrique Nunez

University of Kentucky Doctoral Dissertations

The entire stretch of the Ohio River is under fish consumption advisories due to contamination with polychlorinated biphenyls (PCBs). In this study, natural attenuation and biostimulation of PCBs and microbial communities responsible for PCB transformations were investigated in Ohio River sediments.

Natural attenuation of PCBs was negligible in sediments, which was likely attributed to low temperature conditions during most of the year, as well as low amounts of available nitrogen, phosphorus, and organic carbon. Moreover, surface sediments were relatively oxidized, as indicated by the prevalence of aerobic bacteria such as beta- Proteobacteria, alpha-Proteobacteria, Sphingobacteria, and Nitrospira in 16S rRNA sediment …

Stages Of Relationship Change And Individual And Couple Adjustment, Jacob A. Lacoursiere

University of Kentucky Master's Theses

Although Prochaska and DiClemente (1984) considered the Transtheoretical Model of Change (TTM) to be relevant to couples therapy, there is a paucity of research in this area. Understanding how couples initiate change in their relationship still proves difficult due to barriers in the collection of couple level data and the fact that the majority of research on the TTM is individualistic in nature (Fowers, 2001; Schneider, 2003). Schneider (2003) reported that research suggests a relationship between change processes and relationship adjustment in couples. To my knowledge this study is the first test of the reliability and correlates of relationship change, …

Hypothalamic Mediation Of Acute Increases In Arterial Blood Pressure And Renal Sympathetic Nerve Activity During Electrical Stimulation Of The Lamina Temrinalis, Samuel Paterson Carmichael Ii

University of Kentucky Master's Theses

Discrete electrical stimulation of the organum vasculosum of the lamina terminalis (OVLT) produces sympathetically-mediated increases in peripheral resistance and arterial blood pressure (ABP). Since efferent fibers from the lamina terminalis innervate the kidney through polysynaptic connections, the present study determined whether electrical stimulation of the OVLT increased sympathetic outflow to the kidney. In anesthetized male, Sprague-Dawley rats (n=5) electrical stimulation of OVLT neurons produced frequency and current intensity dependent increases in renal sympathetic nerve activity (RSNA) and ABP that were abolished by ganglionic blockade with the nicotinic antagonist chlorisondamine (5mg/kg,i.v.). Since neurons from the OVLT terminate within the hypothalamic paraventricular …

The Career Of State Sovereign Immunity Under The United States Constitution, William A. Labach

University of Kentucky Master's Theses

controversial since the ratification of the Constitution in 1789. In 1793, the Supreme Court ruled that the states had no sovereign immunity. The Eleventh Amendment reversed this ruling about the Constitution. The Eleventh Amendment itself has also been very controversial. We study the history and development of sovereign immunity jurisprudence from the founding of the United States until the present time.

Removal Of Manganese From An Alkaline Mine Drainage Using A Bioreactor With Different Organic Carbon Sources, Jared D. Edwards

University of Kentucky Master's Theses

The treatment of Mn and SO₄^2- contaminated mine drainage via a sulfate reducing bioreactor is expected to result in near-permanent immobilization of significant amounts of Mn and a portion of the sulfates within the matrix. This study tested several different combinations of organic amendments and inorganic substrates in an attempt to optimize sulfate reducing conditions and Mn removal capacity. Five different organic carbon sources, including corn mash, wood mulch, biosolids, soybean oil, and sorghum syrup in combination with five different inorganic substrates, including creek sediment, marble and limestone chips, polished gravel, and sand were tested in batch experiments. …

Cloning, Characterization And Subcellular Localization Of The N (Nucleocapsid) And P (Phosphoprotein) Protein Of The Sydv (Potato Yellow Dwarf Virus Sanguinolenta Strain), Debasish Ghosh

University of Kentucky Doctoral Dissertations

Potato yellow dwarf virus (PYDV) is the type member of the genus Nucleorhabdovirus. The virus replicates in the nuclei of infected cells and mature virions accumulate in the perinuclear space after viral cores bud through the inner nuclear membrane. The virus was first described as an extremely destructive pathogen of potato (Solanum tuberosum) and other members of family Solanaceae. There are two different strains of PYDV based on their insect-vector specificity, namely SYDV (sanguinolenta strain) and CYDV (constricta strain). PYDV is considered a model system to study virus-vector relationship, particularly for agriculturally harmful rhabdoviruses. However, very little is known about …

Toward The Construction Of "Kentucky Food" In The Twenty-First Century: Food Localism And Commodification Of Place Identity Under Post-Tobacco Agricultural Restructuring, 1990-2006., Taro Futamura

University of Kentucky Doctoral Dissertations

This study examines the concept of local food and the discourses surrounding the concept, both of which have played a significant role during Kentuckys agricultural restructuring. Since the mid-1990s, Kentucky farmers who were dependent on tobacco production began to struggle financially after the substantial reduction of quota allotments, and they were encouraged to diversify their agricultural production. Subsequently, practices of producing, marketing, and consuming locally-grown food were implemented. Drawing primarily on qualitative data, this study investigates the meanings of Kentuckys local food discourse development in four dimensions: 1) the political economy of tobacco production and the structural change of Kentuckys …

A Whole Cell Based Biosensor For Monitoring Physiological Toxins And Early Screening Of Cancer, Gargi Ghosh

University of Kentucky Doctoral Dissertations

Recently a whole cell based biosensor has been developed in our laboratory that consists of a monolayer of human umbilical vein endothelial cells (HUVECs) on the asymmetric cellulose triacetate (CTA) membrane of an ion selective electrode (ISE). When a confluent cell monolayer is formed across the membrane, response from the sensor is inhibited due to inhibited ion transport across the membrane. When the cell based biosensor is exposed to permeability modifying agents, the permeability across the cell monolayer is altered facilitating more ion transport and as a result the response from the sensor increases. This sensor response can be related …

The Disabled Family Dynamic In Drama: The Glass Menagerie, A Day In The Death Of Joe Egg And Time For Ben, Terah Herman

University of Kentucky Master's Theses

Early disability research in the social sciences focused on the individual, or the person with the disability. Only recently has disability research accepted that every family member is affected. The disabled does not suffer the disability alone; the entire family— as well as friends and relatives—suffer ramifications. Parental roles are altered, and grief, anger and guilt often blur the parameters of acceptable parental care. By using disabled family dynamic research in dialog with The Glass Menagerie, A Day in the Death of Joe Egg, and Time for Ben, I argue that the disabled family dynamic is present, …

Harvest Frequency And Cultivar Effects On Yield, Quality, And Regrowth Rate Among New Alfalfa Cultivars, Thomas Adam Probst

University of Kentucky Master's Theses

Alfalfa (Medicago sativa L.) is the most important forage crop in the United States and consistently produces high yields and quality, but harvest frequency is the most significant factor for maximizing forage yield and quality. The objective of this research was to determine forage yield, quality, and regrowth rate among new alfalfa cultivars under four different harvest frequencies. Some of these cultivars have been marketed as having rapid rates of regrowth after cutting to maximize the number of harvests per year. Five cultivars were placed under four harvest frequencies of 25, 30, 35, and 40 days in a split-plot …

Effect Of Dystrophin Deficiency On Selected Intrinsic Laryngeal Muscles Of The Mdx Mouse, Lisa Beth Thomas

University of Kentucky Doctoral Dissertations

The intrinsic laryngeal muscles are recognized as a highly specialized allotype of skeletal muscle. To date, much of the research examining the properties of this muscle group has been conducted on 2 primary muscles: the thyroarytenoid and posterior cricoarytenoid. Consequently, it is unknown whether the remaining intrinsic laryngeal muscles evidence this highly refined phenotype or if they retain a phenotype more similar to prototypical skeletal muscle. The purpose of this study was to further define the biologic properties of the interarytenoid (IA) and cricothyroid (CT) muscles of the larynx using the dystrophin deficient mdx mouse model. Previous work in this …

Routing In Mobile Ad-Hoc Networks: Scalability And Efficiency, Rendong Bai

University of Kentucky Doctoral Dissertations

Mobile Ad-hoc Networks (MANETs) have received considerable research interest in recent years. Because of dynamic topology and limited resources, it is challenging to design routing protocols for MANETs. In this dissertation, we focus on the scalability and efficiency problems in designing routing protocols for MANETs. We design the Way Point Routing (WPR) model for medium to large networks. WPR selects a number of nodes on a route as waypoints and divides the route into segments at the waypoints. Waypoint nodes run a high-level inter-segment routing protocol, and nodes on each segment run a low-level intra-segment routing protocol. We use DSR …

Clinical Functional Testing In People 30-60 Years Old. Expected Performance Values And Correlations To Muscle Fitness And Activity Level, Robert A. (Tony) English

University of Kentucky Doctoral Dissertations

Activity and fitness levels decline through the years from 30 to 60 years of age. Minimal research is available regarding functional assessment tools in this population. Reliable functional tools are needed to reintroduce individuals to appropriate physical activity levels following an injury and to maintain high levels of participation through their lifespan. The purposes of this study were multiple: 1) determine if three functional tests correspond with neuromotor fitness levels, 2) establish a model of functional tests, activity levels and descriptive data that distinguishes the most from the least fit, 3) describe expected mean functional test performances, and 4) demonstrate …

Effects Of Fescue Herbicides Plateau® And Cimarron® On Pregnancy Maintenance In Broodmares And On Alkaloid Concentrations In Endophyte Infected Tall Fescue, Kathleen Scarlett Black

University of Kentucky Master's Theses

Ingestion of endophyte infected (E+) fescue by pregnant mares can cause significant reproductive problems. Plateau® and Cimarron® herbicides suppress fescue while leaving desired forages unharmed. To determine if these herbicides are harmful to pregnant mares, they were allowed to graze pastures treated with Plateau®, Cimarron®, or vehicle carrier. Pregnancies were monitored via ultrasonography, blood chemistry, and hematology. Of the components measured only creatinine differed among treatments over time (P=0.0003) and that increase was only significant in one of four studies.

Two additional experiments were conducted to determine the effect of the herbicides on alkaloids within E+ fescue. A greenhouse experiment …

Being A Man In Kentucky: Perspectives Of Rural Migrant Workers, Mitchell Beam Snider

University of Kentucky Master's Theses

This thesis concerns identity constructions among rural migrant workers in Kentucky in relation to experiences and articulations of transnational spaces, networks, and identities. It was conducted through semi-structured interviews of migrant laborers on two rural Kentucky horse farms with 13 men. In this project, the men’s identities could be seen to have access to and utilize social, economic, cultural, and familial connections across national borders. These aspects of transnational identities were contrasted and compared to aspects of these men’s masculine identities to problematize popular representations of masculinities. This thesis shows how traditional notions of masculinities are questioned, reinforced, discarded, touted …

The Emotions Of Public Housing Policy A Critical Humanist Exploration Of Hope Vi, Ellen Hostetter

University of Kentucky Doctoral Dissertations

Homeownership and Opportunity for People Everywhere VI (HOPE VI) is dramatically changing the face of public housing. The HOPE VI program proposes to replace barracks-style and high rise apartments with a new public housing landscape built on the planning principles of New Urbanism: small-scale developments of single family homes and townhouses with front lawns and porches. Academic and governmental analyses of HOPE VI have used economic, political, and social perspectives to analyze this significant financial investment, radical landscape alteration, and change in residents lives. This dissertation analyzes the process of HOPE VI and its attendant landscapes using a critical humanist …

Zinc Deficiency And Mechanisms Of Endothelial Cell Dysfunction, Huiyun Shen

University of Kentucky Doctoral Dissertations

Atherosclerosis is a chronic inflammatory disease thought to be initiated by endothelial cell dysfunction. Research described in this dissertation is focused on the role of zinc deficiency in endothelial cell activation with an emphasis on the function of the transcription factors nuclear factor-κB (NF-κB), peroxisome proliferator activated receptor (PPAR), and the aryl hydrocarbon receptor (AhR), which all play critical roles in the early pathology of atherosclerosis. Cultured porcine aortic vascular endothelial cells were deprived of zinc by the zinc chelator TPEN and/or treated with the NF-κB inhibitor CAPE or the PPARγ agonist rosiglitazone, followed by measurements of PPARα expression, cellular …

Prostituting The Pulpit? The Negotiated Authority Of Eighteenth-Century New England Clergy, Janice Ellen Wood

University of Kentucky Doctoral Dissertations

Despite the growing population in the colonies throughout the eighteenth century, decreasing numbers of men chose to train for the ministry. New England Congregational clergy not only declined in number; the status, authority and influence enjoyed by their seventeenth-century forbears had drastically declined as well. Early in the century, ministerial authority was bolstered by the clergy’s educational and financial superiority, a virtual monopoly over religious sacraments and the force of localism in small covenanted communities. But the social impact of explosive population growth, a series of currency crises, and warfare throughout the eighteenth century eroded conditions supporting ministerial hegemony.

In …

How, When, And For Whom? Toward A Nuanced Understanding Of The Relation Between African American Ethnic Identity And Mental Health, Ignacio David Acevedo

University of Kentucky Doctoral Dissertations

This study uses a causal modeling approach based on structural equation modeling to examine specific hypotheses regarding the relation among ethnic identity, its components, and various mental health outcomes in African American emerging adults. Data was provided by college students at the Florida Agricultural and Mechanical University. Results did not support a relation between ethnic identity components and adverse mental health. Ethnic identity components were related to positive mental health outcomes; however, this relation differed between gender groups. Among females, the ethnic identity component traditionally labeled ethnic commitment significantly predicted both positive mental health outcomes included in this study. Among …

Exploring A Multidimensional Model Of Victimization And Eating Disturbances For College Women, Malinda Martin Sudduth Isaacs

University of Kentucky Doctoral Dissertations

Research demonstrates that sexual harassment, sexual assault, and eating disorders are pervasive gender-based social problems on college campuses. These phenomena can cause long- term psychological consequences, and negatively impact women’s ability to succeed in both academia and the workplace. Not only have the prevalence and effects of these issues been documented, a significant number of studies have found a relationship between various forms of victimization and eating disorders/symptoms. Research has shown that eating disorders may function as coping strategies for managing the psychological distress that often results from the trauma of sexual harassment and sexual assault. Although, this link has …

The Metastasis Suppressor Nm23-H1 Is Required For Dna Repair, Mengmeng Yang

University of Kentucky Doctoral Dissertations

NM23-H1 represents the first identified metastasis suppressor, exhibiting reduced expression in breast carcinoma and melanoma, and an ability to inhibit metastatic growth without significant impact on the transformed phenotype. Although its molecular mechanism of action is not fully understood, NM23-H1 possesses at least three enzymatic activities that may mediate metastasis suppressor function. It catalyzes nucleoside diphosphate kinase (NDPK) activity, as well as protein histidine kinase and 3’-5’ exonuclease activities. As 3’-5’ exonucleases are generally required for maintenance of genomic integrity, this activity represents a plausible mediator to underlie the metastasis suppressor function of NM23-H1 protein. To investigate the relevant activity …

Evolutionary And Functional Characterization Of Os-Pollux, A Rice Gene Orthologous To A Common Symbiosis Gene In Legume, Cui Fan

University of Kentucky Master's Theses

Root symbioses with arbuscular mycorrhizal fungi and rhizobial bacteria share a common signaling pathway in legumes. Among the common symbiosis genes are CASTOR and POLLUX, the twin homologous genes in Lotus japonicus that encode putative ion channel proteins. Orthologs of CASTOR and POLLUX are ubiquitously present in both legumes and non-legumes, but their function in non-legumes remains to be elucidated. Here, we use reverse genetic approaches to demonstrate that the rice (Oryza sativa) ortholog of POLLUX, namely Os-POLLUX, is indispensible for mycorrhizal symbiosis in rice. Furthermore, we show that Os-POLLUX can restore nodulation, …

Colloid Mediated Transport Of Heavy Metals In Soils Following Reclamation With And Without Biosolid Application, Jarrod O. Miller

University of Kentucky Doctoral Dissertations

Soils disturbed by strip mining practices may have increased colloid loads moving to groundwater resources, also enhancing the transport of contaminants into our water resources. We hypothesize that contaminant transport within soils following mining is enhanced by colloid mobility. Two sites were chosen for this study, a 30-year old reclaimed strip mine in southwest Virginia and a recently mined area from eastern Kentucky. Intact reclaimed soil monoliths were retrieved from sandstone derived soils in southwestern Virginia. Reclaimed monoliths from eastern Kentucky were recreated in the lab. Intact undisturbed (native) soil monoliths representing the soils before mining were also sampled for …

Comparing Personality Disorder Models: Ffm And Dsm-Iv-Tr, Douglas B. Samuel

University of Kentucky Doctoral Dissertations

The current edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-IV-TR; American Psychiatric Association, 2000) defines personality disorders as categorical entities that are distinct from themselves and from normal personality traits. However, many scientists now believe that personality disorders can best be conceptualized using a dimensional model of traits that span normal and abnormal personality, such as the Five-Factor Model (FFM). Many research studies have indicated that the current personality disorder system can be adequately conceptualized using the FFM. However, if the FFM or any dimensional model is to be considered as a credible alternative to …

Performance And Physiology Of Yearling Steers Grazing Toxic Tall Fescue As Influenced By Concentrate Feeding And Steroidal Implants, Jessica Meagan Carter

University of Kentucky Master's Theses

Fescue toxicosis can produce negative effects on animal weight gain and physiology. Sixty-four steers were grazed on endophyte-infected (E+) KY-31 tall fescue for 77 days in 2007 and sixty steers grazed for 86 days in 2008 to evaluate interactions with implantation of steroidal implants and concentrate feeding on performance and physiology of yearling steers. Steers were stratified by body weight for assignment to six, 3.0-ha toxic tall fescue pastures. The main plot treatment of with or without pelleted soybean hulls (SBH) were randomly assigned to pastures. Pelleted SBH were group-fed to provide daily consumptions of 2.3 kg/steer/d (as fed). Sub-plot …

Ecosystem Restoration In The Ouachita National Forest: Evaluating The Pragmatism Of Pre-European Settlement Benchmarks, John Lawrence Davenport

University of Kentucky Doctoral Dissertations

This paper looks at the intersections of nature and culture through a study of forest ecosystem restoration efforts in the Ouachita National Forest (Arkansas and Oklahoma). Ecosystem restoration goals are often informed by a pre-European settlement (PES) condition, with an implicit (and occasionally explicit) assertion that such conditions are both more natural than and preferable to the contemporary state. In many cases resuming pre-suppression fire regimes remains a key mechanism for achieving this restored condition. This study’s three main objectives include: (1) determining how PES benchmarks arose in restoration thought, (2) examining how the choice to use a PES benchmark …

Design, Synthesis, And Pharmacological Evaluation Of A Series Of Novel, Guanidine And Amidine-Containing Neonicotinoid-Like Analogs Of Nicotine: Subtype-Selective Interactions At Neuronal Nicotinic-Acetylcholine Receptor., Aaron Joseph Haubner

University of Kentucky Doctoral Dissertations

The current project examined the ability of a novel series of guandine and amidine-containing nicotine analogs to interact with several native and recombinantlyexpressed mammalian neuronal nicotinic-acetylcholine receptor (nAChR) subtypes. Rational drug design methods and parallel organic synthesis was used to generate a library of guanidine-containing nicotine (NIC) analogs (AH compounds). A smaller series of amidine-containing nicotine analogs (JC compounds) were also synthesized. In total, >150 compounds were examined. Compounds were first assayed for affinity in a high-throughput [³H]epibatidine radioligand-binding screen. Lead compounds were evaluated in subtype-selective binding experiments to probe for affinity at the α₄β_{2 …}