Digital Commons Network^™

Stability Of Spore-Based Sensing Systems, Abhishek Sangal

University of Kentucky Master's Theses

The full exploitation of bacterial whole-cell biosensing systems in field applications requires the survival of bacterial cells and long term-preservation of their sensing ability during transportation and on-site storage of such analytical systems. Specifically, there is a need for rapid, simple and inexpensive biosensing systems for monitoring human health and the environment in remote areas which often suffer from harsh atmospheric conditions and inadequate commercial distribution and storage facilities. Our laboratory has previously reported the successful use of bacterial spores as vehicles for the long-term preservation and storage of whole-cell biosensing systems at room temperature.

In the present research, we …

Screen Door Medicine: The Informal Medical Consultation, Debra Faith Nickell

University of Kentucky Doctoral Dissertations

This study explores the phenomenon of the informal medical consultation, a communication event in which an individual asks for medical information, advice, or care from an off-duty health professional with whom the individual has no formal patient-provider relationship. Using surveys and interviews, the study describes these consultations from the perspective of the health care professional and the informal patient. The study explores foundational theories that offer explanations for the phenomenon. The theories considered include social support, decision-making, social exchange, perceived partner responsiveness to needs, and uncertainty management.

This study suggests health care providers perceive informal medical consultations to be more …

Career Interruptions: Wage And Gender Effects, Jill Kearns

University of Kentucky Doctoral Dissertations

This dissertation examines the effects of career interruptions on workers’ wages. In chapter four I examine whether controlling for the type of interruption differently affects men’s and women’s wages and therefore can be used to explain the remaining gender wage differences. The increased participation of married women in the labor force has increased their wages from just 30% of men’s wages in 1890 to nearly 80% as of 2001. Thus, although the gender wage gap has narrowed over time, it has yet to be eliminated. One argument for the persistence of the gender wage gap is that previously researchers have …

Shame, Guilt, And Knowledge Of Hpv In Women Recently Diagnosed With Hpv-Related Cervical Intraepithelial Neoplasia (Cin), Sarah E. Flynn

University of Kentucky Doctoral Dissertations

The current study investigated the relationships between state shame, guilt, and disease knowledge in women recently diagnosed with cervical intraepithelial neoplasia (CIN) caused by the human papillomavirus (HPV). Recent research has indicated that diagnosis of HPV can elicit negative self-directed affect, including persistent experiences of shame. Studies have also shown that knowledge of HPV is low in the general population, even though it is the most common sexually transmitted infection. It is important to understand how shame affects those with HPV because shame is related to a decline in important immune parameters that may be essential in HPV clearance. A …

Inhibition Of Cholesterol Synthesis By Policosanol, Subhashis Banerjee

University of Kentucky Doctoral Dissertations

Cholesterol is an essential component of the cell, but excessive blood levels are a major risk factor for the development of atherosclerotic plaques that can lead to heart disease and stroke, the foremost cause of premature death in Western societies. Policosanol, a mixture of very long chain alcohols derived from sugarcane, has gained considerable attention among the public as safe and effective means to reduce blood cholesterol levels, a belief based on some early clinical studies. My research investigates one possible mechanism by which policosanol might decrease blood cholesterol levels: the inhibition of cholesterol synthesis in the liver. Previous studies …

Genetic Diversity, Structure, And Recolonization Patterns Of Kentucky Black Bears, John Tyler Hast

University of Kentucky Master's Theses

After nearly a century of absence, the black bear (Ursus americanus) reappeared in Kentucky during the late 20th century and has since increased in number. Recolonization of bears in the southeastern portion of the state was thought to have been caused by emigration of bears from adjacent states into the Commonwealth, while in the south-central area, bears originated, or natural recolonization may have been supplemented by the translocation of 14 individuals into the Big South Fork National River Recreation Area. To investigate the recolonization patterns of bears in Kentucky, I used 20 microsatellite loci to determine the genetic …

Distribution Of Eastern Hemlock, Tsuga Canadensis, In Eastern Kentucky And The Susceptibility To Invasion By The Hemlock Woolly Adelgid, Adelges Tsugae, Joshua Taylor Clark

University of Kentucky Master's Theses

The hemlock woolly adelgid, an invasive non-native insect, is threatening eastern hemlock in Kentucky. This study examined three techniques to map the distribution of eastern hemlock using decision trees, remote sensing, and species distribution modeling. Accuracy assessments showed that eastern hemlock was best modeled using a decision tree without incorporating satellite radiance. Using the distribution from the optimal model, risk maps for susceptibility to hemlock woolly adelgid infestation were created using two species distribution models. Environmental variables related to dispersal were used to build the models and their contributions to the models assessed. The models showed similar spatial distributions of …

Health Promotion And Health Education: Nursing Students’ Perspectives, Kathleen Ann Halcomb

University of Kentucky Doctoral Dissertations

The purpose of this study was to determine student nurses’ perceptions of (1) the role of the nurse in health promotion, and (2) how the concept of health promotion is presented in nursing curricula. Research questions for this study included the following: 1) Can nursing students explain the difference between health education and health promotion? 2) What have nursing students been exposed to within their curriculum regarding health promotion? 3) What health promoting behaviors are nursing faculty role modeling as perceived by nursing students? 4) What is the role of the nurse in implementing health promotion as perceived by nursing …

Cycling As A Political Act: The Framing And Culture That Create A New Social Movement, Mitchael Lee Schwartz

University of Kentucky Master's Theses

This study analyzes the bicycling community of Lexington, Kentucky. Interviews and participant observation were conducted in order to better understand the structure of Lexington’s cycling community, revealing three prominent groups/types of cyclists: (1) road cyclists, (2) underground/urban cyclists, and (3) commuters. The characteristics of each group are discussed, with particular attention devoted to the underground/urban cyclists, due to their politically-minded culture. Building from prior social movement literature, the unique framing processes of the underground/urban cycling group are analyzed in order to explore the group as a new social movement. Finally, the potential for a broader cycling movement based upon interests …

The Effect Of Acupressure On Constipation, Quality Of Life, And Depressive Symptoms In Cancer Patients With Constipation, Eun Jin Lee

University of Kentucky Doctoral Dissertations

Constipation is the most common gastrointestinal complaint in the United States, resulting in about 2 million annual visits to the doctor.

The purposes of this dissertation were to: 1) describe four theories of traditional Korean medicine; 2) review mechanisms of acupuncture and moxibustion and develop a conceptual model; 3) review findings from randomized controlled trials that tested the effects of acupressure used for the management of symptoms such nausea, pain, and dyspnea; 4) examine the reliability and validity of the Patient Health Questionnaire-9 (PHQ-9) in heart failure patients with constipation compared to those with gastrointestinal disease; 5) describe how Korean …

Auditory Training At Home For Adult Hearing Aid Users, Anne D. Olson

University of Kentucky Doctoral Dissertations

Research has shown that re-learning to understand speech in noise can be a difficult task for adults with hearing aids (HA). If HA users want to improve their speech understanding ability, specific training may be needed. Auditory training is one type of intervention that may enhance listening abilities for adult HA users.

The purpose of this study was to examine the behavioral effects of an auditory training program called Listening and Communication Enhancement (LACE™) in the Digital Video Display (DVD) format in new and experienced HA users. No research to date has been conducted on the efficacy of this training …

La Mujer Se Va Pa’Bajo: Women’S Health At The Intersections Of Nationality, Class, And Gender, Mary Alice Scott

University of Kentucky Doctoral Dissertations

This research utilizes an intersectionality framework to examine the complexity of social location and its effects on women's health. By examining connections among the state, processes of globalization, and the production of health inequalities for poor women in a rural community in southern Veracruz, Mexico, the research highlights the nexus of nationality, class, and gender. Four interconnected contexts are explored: (1) women's increasing paid and unpaid labor in the context of a poverty of resources brought on by sustained economic crisis; (2) the maintenance of reproductive labor as the responsibility of women; (3) the development of migrant "illegality" and its …

An Empirical Analysis Of Reputation Effects And Network Centrality In A Multi-Agency Context, Emily Jane Plant

University of Kentucky Doctoral Dissertations

Signals convey information to marketplace participants regarding the unobservable quality of a product. Whenever product quality if unobservable prior to purchase, there is the risk of adverse selection. Problems of hidden information also occur in the consumer marketplace when the consumer is unable to verify the quality of a good prior to purchase. The sending, receiving, and interpretation or signals are potential ways to overcome the problem of adverse selection. In general, there is a lack of empirical evidence for signaling hypothesis, particularly that which links signaling to business performance outcomes. This research proposes that reputation serves as a marketplace …

Drug Release And Pharmacokinetic Properties Of Liposomal Db-67, Yali Liang

University of Kentucky Master's Theses

Sterically stabilized liposomes with saturated lipid as the major lipid component (DSPC:m-PEGDSPE were applied in DB-67 delivery. The drug retention in vitro and pharmacokinetic properties in vivo were investigated. Liposomal DB-67 was cleared faster from the circulation in the larger liposomes (~180 nm) than in the smaller ones (~120 nm), even though DB-67 was retained longer in smaller size liposomes in vitro. Liposomal DB-67 clearance was increased when cholesterol was present in the liposomal composition (40 mole %). It can be attributable to the faster drug release from cholesterol containing liposomes as compared to liposomes without cholesterol. Cholesterol free …

The Role Of The L-Type Calcium Channel And Its Carboxyl-Terminus, Miranda Jean Byse

University of Kentucky Doctoral Dissertations

In the heart, the primary role of the L-type calcium channel (LTCC) Ca_V1.2 is to conduct calcium into cardiomyocytes and initiate contraction. However, part of the Ca_V1.2 channel itself, the cleaved carboxyl-terminus (CCt) can also localize to the nucleus and regulate gene transcription. Therefore, the goal of this dissertation project was to determine the role and regulation of CCt in the embryonic and adult heart. The global hypothesis of my dissertation project is that CCt localizes to the nucleus in embryonic and adult cardiomyocytes via a calcium-mediated mechanism and regulates transcription. A model of pharmacological LTCC …

Tobacco-Free Prison Policies And Health Outcomes Among Inmates, Alison R. Connell

University of Kentucky Doctoral Dissertations

This study was the first to examine the effect of tobacco policies in prisons on the health of inmates. Kentucky has two types of tobacco policies in its 16 state prisons: indoor smoke-free policies, where smoking is allowed outdoors and tobacco-free policies, in which no tobacco of any kind is allowed on the grounds of the prison. The smoking rate of inmates is three times higher than that of current smokers in the non-incarcerated population which results in high rates of tobacco-related health conditions such as heart disease and lung cancer.

A literature review discussed the evolution of tobacco policies …

Critical Geopolitics Of Islam In Astrakhan, Russia: Mosque Construction And Community Building, Meagan Lucinda Todd

University of Kentucky Master's Theses

This thesis examines how and under what influences communities of Islamic faith have developed in post-Soviet Russia. My arguments are based on research conducted in Astrakhan, Russia in the summer of 2009. Astrakhan is the capital of Astrakhan Oblast in southwest Russia and has a reputation for being a multi-confessional and multi-ethnic city. Astrakhan is home to Russians, Tatars, Kazakhs, Kalmyks, and many other nationalities. I draw from interviews and newspaper analysis to examine what the local landscape of Islam looks like in Astrakhan, how has it changed since the collapse of the USSR, and what future trends are emerging. …

Institutional Elder Neglect In Civil Court: Perceptions Of Video Recorded Victim Testimony, Nesa Elizabeth Wasarhaley

University of Kentucky Master's Theses

Mock juror perception of institutional elder neglect (IEN) was investigated in a civil court context. Participants (N=148) read a fictional IEN civil trial summary in which an alleged elderly female victim filed a lawsuit against her nursing home for failure to provide adequate care but died prior to trial. Participants read a version in which (a) previously recorded video testimony from the alleged victim was presented, (b) the alleged victim’s floor-mate testified about witnessing the neglect, or (c) no witness testimony was presented. An ageism scale was completed, and participants indicated the amount of time they spend with elders. Results …

Engineering Sport Safety: A Study Of Equestrian Cross Country Eventing, Katherine M. Kahmann

University of Kentucky Master's Theses

The sport of equestrian cross country eventing has seen many serious and even fatal injuries due to rotational horse falls in recent years. The sport originally consisted of horse and rider teams jumping stationary, wood fences. However, in a move towards increasing safety for horses and riders, frangible and deformable safety devices have been emerging in the field. This thesis provides an overview of safety designs that are currently available and those that are on the horizon. Also, a path-finder method of evaluating and developing safety fence designs was outlined and applied to two distinct designs, a hinged gate and …

Globalization And The Politics Of The Welfare State, Hanbeom Jeong

University of Kentucky Doctoral Dissertations

The theoretical argument of this study is that economic globalization, by default, exerts a downward pressure on the social policies of states largely through the operations of transnational corporations. However, since globalization’s effect on social policy is conditional on endogenous political forces such as regime type, democratization, electoral competition and political participation, its proclivity to retrench the welfare state is averted by the preferences of political actors and institutions to expand social spending. This argument found consistent empirical support via a series of cross-section regressions that estimated the interactive effects of economic globalization and various measures of domestic political institutions …

Essays On Human Capital, Health Capital, And The Labor Market, Charles Hokayem

University of Kentucky Doctoral Dissertations

This dissertation consists of three essays concerning the effects of human capital and health capital on the labor market. Chapter 1 presents a structural model that incorporates a health capital stock to the traditional learning-by-doing model. The model allows health to affect future wages by interrupting current labor supply and on-the-job human capital accumulation. Using data on sick time from the Panel Study Income of Dynamics the model is estimated using a nonlinear Generalized Method of Moments estimator. The results show human capital production exhibits diminishing returns. Health capital production increases with the current stock of health capital, or better …

Polychlorinated Biphenyl-Induced Endothelial Cell Dysfunction And Its Modulation By Dietary Lipids, Zuzana Majkova

University of Kentucky Doctoral Dissertations

Cardiovascular diseases are the number one cause of death in Western societies. Endothelial dysfunction is an early event in the pathology of atherosclerosis, which is an underlying cause in the majority of cardiovascular events. Exposure to persistent environmental pollutants, such as polychlorinated biphenyls (PCBs), is a risk factor for the development of atherosclerosis.

First, we tested a hypothesis that coplanar PCBs, dioxin-like chemicals with affinity for aryl hydrocarbon receptor (AhR), can stimulate up-regulation of monocyte chemoattractant protein-1 (MCP-1), an endothelium-derived chemokine that attracts monocytes into sub-endothelial space in early stages of atherosclerosis. Coplanar PCBs 77 and 126 increased expression of …

Alpha7 Nicotinic Acetylcholine Receptor Regulation In Experimental Neurodegenerative Disease, Christina Margaret Charriez

University of Kentucky Doctoral Dissertations

The α7 nicotinic acetylcholine receptor (nAChR) is involved in learning and memory, synaptic plasticity, neuroprotection, inflammation, and presynaptic regulation of neurotransmitter release. Alzheimer’s disease (AD), a neurodegenerative disease characterized by diminished cognitive abilities, memory loss, and neuropsychiatric disturbances, is associated with a loss of nAChRs. Similarly, traumatic brain injury (TBI) may result in long term neurobehavioral changes exemplified by cognitive dysfunction. Deficits in α7 nAChR expression have previously been shown in experimental TBI and may be related to cognitive impairment experienced in patients following TBI.

The purpose of this dissertation was to investigate changes in α7 nAChR expression in models …

Survival And Inflammation In Patients With Heart Failure: The Impact Of Overweight, Obesity, Diabetes And Fruit And Vegetable Consumption, Heather Payne-Emerson

University of Kentucky Doctoral Dissertations

Overweight and obesity are paradoxically associated with better survival in patients with heart failure (HF). This association is poorly understood, in part because the impact of diabetes (DM) on survival of overweight and obese HF patients has not been considered. Inflammation may contribute to worse survival in overweight and obese HF patients with DM, and levels of inflammation may be associated with fruit and vegetable consumption. However, neither of these relationships has been investigated in patients with HF.

The purposes of this dissertation were to a) examine the effect of DM on survival of overweight and obese patients with HF, …

Exotic Invasive Plants In Kentucky, Yu Liang

University of Kentucky Master's Theses

Invasion of exotic species is a significant problem in natural ecosystems, reaching epidemic proportions and resulting in significant economic losses. However, insufficient knowledge of explicit spatial distribution of invasive species hinders our ability to prevent and/or mitigate future invasion. In this study, we demonstrate the use of existing voluntary data to survey invasive plant species in Kentucky. We also reconstructed the historical distribution of 16 exotic invasive plants typical to Kentucky using herbarium records. We found that Kentucky is facing a large threat from exotic invasive plants as they are reported throughout most counties. The distribution maps for four of …

Sustainable Resource Utilization In Manufacturing Of Printed Circuit Board Assembly: Exergy Analysis Of The Process, Subramaniam Saiganesh

University of Kentucky Master's Theses

Engineering for sustainable development requires prudent utilization of resources under economic, environmental and societal constraints. Resource utilization must follow a holistic approach. This brings in a need for comprehensive metrics which are simple, standard and universal. Thermodynamics may offer a metric that focuses on both quality and quantity of energy resources which may carry information to be combined with other metrics. This metric may be a thermodynamic property called exergy or available energy, which provides a better insight into resource use in both energy and non-energy producing systems. This thesis is devoted to a study of the exergy concept in …

Biochemical Characterization Of Human Mismatch Recognition Proteins Mutsα And Mutsβ, Lei Tian

University of Kentucky Doctoral Dissertations

The integrity of an organism's genome depends on the fidelity of DNA replication and the efficiency of DNA repair. The DNA mismatch repair (MMR) system, which is highly conserved from prokaryotes to eukaryotes, plays an important role in maintaining genome stability by correcting base-base mismatches and insertion/deletion (ID) mispairs generated during DNA replication and other DNA transactions. Mismatch recognition is a critical step in MMR. Two mismatch recognition proteins, MutSα (MSH2-MSH6 heterodimer) and MutSβ (MSH2-MSH3 heterodimer), have been identified in eukaryotic cells. MutSα and MutSβ have partially overlapping functions, with MutSα recognizing primarily base-base mismatches and 1-2 nt ID mispairs …

The Relationship Between Information Technology And Construction Productivity, Dong Zhai

University of Kentucky Doctoral Dissertations

Over the past decades, information technology has been impacting industries, economics, the way of life and even the culture throughout the world. Productivity has been attracting much attention as an important indicator of economics, and numerous researchers have investigated the relationship between information technology and productivity. Construction is one of the largest industries in the United States, but little research has been conducted to investigate the relationship between information technology and construction productivity.

The major objective of this dissertation is to determine the degree (if any) to which information technology usage, specifically the use of information technology to automate and …

Neural Mechanisms Of Sympathetic Activation During Hyperinsulinemia And Obesity-Induced Hypertension, Megan Elyse Bardgett

University of Kentucky Doctoral Dissertations

Obesity afflicts more than 30% of the U.S. population and is a major risk factor for the development of hypertension, type II diabetes, and cardiovascular disease. Studies in humans and animals indicate that obesity is associated with increased sympathetic outflow to the vasculature and kidneys. One mechanism postulated to underlie the increase in sympathetic nerve activity (SNA) in obesity is hyperinsulinemia. Little is known regarding the central circuitry underlying elevated SNA and arterial blood pressure (ABP) during hyperinsulinemia and obesity or if sympathoexcitatory circuits are still responsive to insulin in obesity.

Hyperinsulinemic-euglycemic clamps elevate SNA to the hind limb vasculature …

Stalking Myth Acceptance: An Investigaton Of Attitudinal Constructs Associated With Gender Differences In Judgments Of Intimate Stalking, Emily Elizabeth Dunlap

University of Kentucky Doctoral Dissertations

Emerging research has shown that women and men perceive criminal stalking differently, yet there is little research addressing why these differences exist. For example, mock juror research on intimate stalking has found that men are more likely than women to render lenient judgments (e.g., not-guilty verdicts). Understanding the underlying attitudes associated with differences in how men and women interpret whether certain behaviors would cause reasonable fear is crucial to an evaluation of current anti-stalking legislation. The primary goals of this research were: (1) to examine the extent to which beliefs that support stalking (i.e., stalking myth acceptance – SMA victim …