Digital Commons Network^™

Teacher Disclosure: Developing Privacy Rules, Managing Boundaries And Building Relationships, Renee Monique Kaufmann

University of Kentucky Master's Theses

The roles and responsibilities of middle school teachers are never ending. From instructing students on state-mandated curriculum to the enrichment of character and inquiry, teachers make daily decisions about how and what to disclose to their students. The current study reexamines Hosek and Thompson’s (2009) study on how teachers develop privacy rules and coordinate boundaries using Petronio’s Communication Privacy Management as the theoretical framework. Studying middle school teachers, in lieu of college instructors, allows for a better understanding of how privacy rules and boundaries are constructed and used within the middle school. This provides a better understanding of the important …

Self-Regulation And Liver Function: Expanding An Ecological Model, Tory Anne Eisenlohr-Moul

University of Kentucky Master's Theses

Under conditions of high self-regulatory effort, peripheral organ systems have been found to slow, potentially to rearrange energetic priorities in favor of the brain. The present study tested an expansion of this model by exploring the possibility that alcohol metabolism (i.e., liver function) may slow during self-regulation. We also anticipated that high trait self-control would attenuate the effect of condition on metabolism. Twelve males aged 21-25 completed two conditions in counterbalanced order. During each session, the participant received 0.33 ml/kg of absolute alcohol for a target peak blood alcohol concentration (BAC) of 0.03 g%. Participants then performed tasks (self-regulatory tasks …

Solution Phase And Membrane Immobilized Iron-Based Free Radical Reactions: Fundamentals And Applications For Water Treatment, Scott Romak Lewis

University of Kentucky Doctoral Dissertations

Membrane-based separation processes have been used extensively for drinking water purification, wastewater treatment, and numerous other applications. Reactive membranes synthesized through functionalization of the membrane pores offer enhanced reactivity due to increased surface area at the polymer-solution interface and low diffusion limitations. Oxidative techniques utilizing free radicals have proven effective for both the destruction of toxic organics and non-environmental applications. Most previous work focuses on reactions in the homogeneous phase; however, the immobilization of reactants in membrane pores offers several advantages. The use of polyanions immobilized in a membrane or chelates in solution prevents ferric hydroxide precipitation at near-neutral pH, …

Implementation Effectiveness Of Campus Tobacco-Free Policies, Amanda Fallin

University of Kentucky Doctoral Dissertations

Tobacco use and secondhand smoke exposure are leading causes of preventable morbidity and mortality in the United States. Outdoor tobacco smoke exposure conveys many of the same risks as indoor secondhand smoke exposure. Tobacco-free campuses policies are an intervention to promote a positive social norm that encourages smoking cessation, as well as reduces exposure to outdoor tobacco smoke. This dissertation contains a review of the policy implementation literature; findings from a psychometric analysis of the newly developed Tobacco-free Compliance Assessment Tool (TF-CAT) to assess compliance with tobacco-free campus policies; and results of a campus intervention study to promote compliance. The …

Connections, Paths, And Explanations - A Social Network Approach To Investigating Experiences Of Early Childhood Special Education With The Ecls-K, Kathryn Shirley Akers

University of Kentucky Doctoral Dissertations

The purpose of this study is to demonstrate a practical application of social network analysis in the field of education using a large-scale data source. Using the Early Childhood Longitudinal Base Year data, a network is identified by examining the connections that occur between supports, both inside and outside formal special education resources for kindergarteners with access to special education programs.

Social network mappings and quantitative findings are presented for formal and informal supports and primary disability category, along with policy implications and suggestions for further research. Findings indicate that social network analysis offers a unique and innovative perspective to …

Lycopene And Its Potential Nutritional Role For Patients With Heart Failure, Martha J. Biddle

University of Kentucky Doctoral Dissertations

Lycopene is a antioxidant found in natural and processed foods. The connection between antioxidants and heart disease has been explored in several observational studies^1-4, yet very few investigators have examined the impact of dietary antioxidants in patient with advanced heart disease such as heart failure (HF). A novel strategy for preventing or delaying the complications of HF related to inflammation and oxidative stress may be to increase dietary lycopene.

The purpose of this dissertation was to test the impact of dietary intervention consisting of lycopene (V8® juice) on biomarkers of inflammation and oxidative stress in patients with HF; …

Towards The Total Synthesis Of The Capuramycin Family Of Natural Products, Jesse M. Jacobsen

University of Kentucky Master's Theses

Despite over a century of advancement, tuberculosis remains a grave threat to world health. In particular, third world countries continue to struggle with the crushing weight of the disease. Furthermore, the emergence of drug resistance in TB strains poses a significant threat to the first world where incidence and mortality is low. The dwindling efficacy of current drug regimens necessitates research into new small molecules capable of arresting the growth and spread of TB. The capuramycin family of nucleoside antibiotics shows strong potential to become part of this new generation of anti-TB small molecules. Indeed, their ability to inhibit Translocase …

Social Capital, Solidarity, And Cohort Effect —An Analysis Of The Production Of Social Capital Among Union Miners In Harlan County, Kentucky, Feng Hao

University of Kentucky Master's Theses

The coal industry exercises a pervasive influence on mining communities in Appalachia, even though it exerts enormous damages on the environment and makes limited contributions to employment and the advancement of the communities. One explanation for this paradox offered by Bell is a depletion of social capital among coalfield residents in Central Appalachia (2009). Her data suggests that the “ripping away” of the region’s strong union identity lead to a resocialization, “from a ‘we’ mentality to an ‘I’ mentality, thus demising the store of social capital” (2009:655). My research aims to interpret how social capital resources among union miners was …

Nozzle Type And Arrangement Alternatives For Improved Application Of Suckercides In Burley Tobacco (Nicotiana Tabacum L.), Beau Robert Neal

University of Kentucky Master's Theses

Maleic hydrazide (MH) applications have been standard practice for sucker control in burley tobacco (Nicotiana tabacum L.) production for the last half-century because it is relatively inexpensive, effective and easy to apply. Non-MH suckercides such as fatty alcohols and local systemics can be utilized to reduce or replace MH and lower undesirable residues in the cured leaf. The objective of this study was to evaluate various nozzle types and arrangements for efficiency to determine if sucker control with fatty alcohol could be consistently improved over the currently used TG3-5-3 arrangement, as well as examine sprayer positioning (center vs. off-center) …

The Influence Of Antidiabetic Medications On The Development And Progression Of Prostate Cancer, Anna Elizabeth Hitron

University of Kentucky Master's Theses

The development of prostate tumors has been linked to co-morbid diabetes mellitus (DM) in several studies, potentially through the stimulation of insulin-like growth factor receptor (IGFR). This study evaluates the effect of anti-diabetic medication use on the development of high grade tumors and time to tumor progression compared to non-diabetics. This retrospective, nested case control study identified patients with prostate cancer (PCa) from the Kentucky Medicaid Database. Cases were diagnosed with PCa and DM and using at least one of the following antidiabetic medications; sulfonylureas, insulin, metformin or TZDs. Cases were further stratified on their insulin exposure resulting from therapy. …

Evaluation Of The Physicochemical Properties And Stability Of Solid Lipid Nanoparticles Designed For The Delivery Of Dexamethasone To Tumors, Melissa Howard

University of Kentucky Doctoral Dissertations

Pre-clinical and clinical trials suggest that pre-treatment with dexamethasone (Dex) may facilitate enhanced uptake of subsequently administered chemotherapeutic agents. To reduce the side effects associated with systemic administration of Dex, solid lipid nanoparticles (SLNs) containing dexamethasone palmitate (Dex-P) were prepared as a means of achieving tumor-targeted drug delivery. These studies were aimed at evaluating the physicochemical properties and both the physiological and storage stability of the SLNs.

SLNs were prepared using nanotemplate engineering technology. Stearyl alcohol (SA) was used as the lipid phase with Brij^® 78 and Polysorbate 60 as surfactants and PEG6000 monostearate as a long-chain PEGylating agent. …

Genome-Wide Association Studies At The Interface Of Alzheimer’S Disease And Epidemiologically Related Disorders, Christopher Ryan Simmons

University of Kentucky Doctoral Dissertations

Genome-wide association studies (GWAS)s provide an unbiased means of exploring the landscape of complex genetic disease. As such, these studies have identified genetic variants that are robustly associated with a multitude of conditions. I hypothesize that these genetic variants serve as excellent tools for evaluation of the genetic interface between epidemiologically related conditions. Herein, I test the association between SNPs associated with either (i) plasma lipids, (ii) rheumatoid arthritis (RA) or (iii) diabetes mellitus (DM) and late-onset Alzheimer’s disease (AD) to identify shared genetic variants. Regarding the most significantly AD-associated variants, I have also attempted to elucidate their molecular function. …

Analysis Of Two-Year Colleges: Transfer, Retention And Graduation, Darshak P. Patel

University of Kentucky Doctoral Dissertations

Investment in higher education is typically considered as a static discrete-choice problem where students make post-secondary education choices usually right after high school (Heckman et al., 2006). This is largely aligned with Becker’s human capital theory. As Becker’s theory holds, students’ decisions can alter with the arrival of new information (Weisbrod, 1964). By relaxing the assumption certainty in the human capital model, student education decisions can be modeled using Weisbrod’s option value theory. According to this theory, students reevaluate their lifetime-utility maximizing decisions based on new information acquired in a sequential nature. Students face large uncertainties due to unexpected positive …

Advanced Studies On Series Impedance In Waveguides With An Emphasis On Source And Transfer Impedance, Jinghao Liu

University of Kentucky Doctoral Dissertations

Series impedances, including source and transfer impedances, are commonly used to model a variety of noise sources and noise treatment elements in duct systems. Particle velocity is assumed to be constant on the plane where the series impedances are defined. The research reported herein details investigations into measuring source and transfer impedance. Especially, the measurement and prediction of the transfer impedance of micro-perforated panel (MPP) absorbers is considered.

A wave decomposition method for measuring source impedance and source strength was developed that was purely based on acoustic concepts instead of the equivalent circuit analysis. The method developed is a two-load …

Essays On Income Volatility And Individual Well-Being, Bradley L. Hardy

University of Kentucky Doctoral Dissertations

My dissertation consists of three essays in which I document trends in earnings and income volatility, estimate potential causal mechanisms for changing volatility, and examine the long-term consequences of parental income volatility for children. In essay 2 I document trends in earnings and income volatility of individuals and families using matched data in the March Current Population survey from 1973 to 2009. Essay 3 advances the literature on volatility, using matched data from the CPS to identify demographic and labor market correlates of earnings volatility within education-birth year cohorts. This study collapses the cross-sectional CPS into a pseudo-panel and then …

Identification Of Activities Involved In Cag/Ctg Repeat Instability, Nelson Lap Shun Chan

University of Kentucky Doctoral Dissertations

CAG/CTG repeat instability is associated with at least 14 neurological disorders, including Huntington’s disease and Myotonic dystrophy type 1. In vitro and in vivo studies have showed that CAG/CTG repeats form a stable hairpin that is believed to be the intermediate for repeat expansion and contraction.

Addition of extra DNA is essential for repeat expansion, so DNA synthesis is one of the keys for repeat expansion. In vivo studies reveal that 3’ CTG slippage with subsequent hairpin formation (henceforth called the 3’ CTG slippage hairpin) occurs during DNA synthesis. It is proposed that hairpin tolerance machinery is activated because prolonged …

Estimation Of Pedestrian Safety At Intersections Using Simulation And Surrogate Safety Measures, Nithin K. Agarwal

University of Kentucky Doctoral Dissertations

With the number of vehicles increasing in the system every day, many statewide policies across the United States aim to increase the use of non- motorized transportation modes. This could have safety implications because the interaction between motorists and non-motorists could increase and potentially increasing pedestrian-vehicle crashes. Few models that predict the number of pedestrian crashes are not sensitive to site-specific conditions or intersection designs that may influence pedestrian crashes. Moreover, traditional statistical modeling techniques rely extensively on the sparsely available pedestrian crash database.

This study focused on overcoming these limitations by developing models that quantify potential interactions between pedestrians …

Role Of Lipids In Tombusvirus Replication, Monika Sharma

University of Kentucky Doctoral Dissertations

Positive-strand RNA virus group are the most abundant among viruses affecting plants and animals. To successfully achieve replication, these viruses usurp or co-opt host proteins. To facilitate the discovery of host factors involved in Tomato bushy stunt virus (TBSV), yeast has been developed as a surrogate model host. Genome-wide approaches covering 95% of yeast genes, has revealed approximately hundred factors that could affect virus replication. Among the identified host factors, there are fourteen yeast genes, which affect/regulate lipid metabolism of the host.

One of the identified host gene is ERG25, which is an important factor for sterol biosynthesis pathway, affecting …

Dementia Caregiving Outcomes: The Impact Of Caregiving Onset, Role Occupancy, And Care-Recipient Decline, Katherina Nikzad-Terhune

University of Kentucky Doctoral Dissertations

Dementia is characterized as a progressive loss of brain function that results in the deterioration of many cognitive and physical abilities. Alzheimer’s disease (AD) is the most common form of dementia, causing steady declines in memory, functional abilities, and mental functioning. With a projected increase of degenerative illnesses, such as AD, family caregiving for individuals with the disease is also steadily increasing. Caring for an individual with AD has been characterized as a “career,” and within this career are a number of key transitions, including the onset of caregiving. Preexisting caregiving research reveals a number of negative consequences for AD …

Molecular And Genomic Approaches To Understanding Host-Virus Interactions In Shaping The Outcome Of Equine Arteritis Virus Infection, Yun Young Go

University of Kentucky Doctoral Dissertations

Equine arteritis virus (EAV) is the causal agent of equine viral arteritis, a disease of equids. During natural outbreaks of the disease, EAV can cause abortion in pregnant mares and persistent infection in stallions. Understanding how host cellular proteins interact with viral RNA and viral proteins, as well as their role in viral infection, will enable better characterization of the pathogenesis of EAV and establishment of persistent infection in stallions. Accordingly, we hypothesized that both viral factors and host genetically related factors could influence the outcome of EAV infection in horses. To test this hypothesis, we first combined contemporary molecular …

Characterization Of G10h Promoter And Isolation Of Wrky Transcription Factors Involved In Catharanthus Terpenoid Indole Alkaloid Biosynthesis Pathway, Nitima Suttpanta

University of Kentucky Doctoral Dissertations

Catharanthus roseus produces a large array of terpenoid indole alkaloids (TIAs) that are an important source of natural or semi-synthetic anticancer drugs. Biosynthesis of TIAs is tissue-specific and induced by certain phytohormones and fungal elicitors, indicating the involvement of a complex transcriptional control network. However, the transcriptional regulation of the TIA pathway is poorly understood. This study reports the isolation and characterization of the G10H promoter and two WRKY transcription factors regulating TIA biosynthesis.

Geraniol 10-hydroxylase (G10H) controls the first committed step in the biosynthesis of terpenoid indole alkaloids (TIA). The C. roseus G10H promoter sequence was isolated by a …

Polyamine Modulation In Alcoholism: Examination Using A Novel Screening Procedure Designed To Predict Anti-Relapse And Neuroprotective Efficacy, J. Ben Lewis

University of Kentucky Doctoral Dissertations

Alcohol dependence is a major public health concern. Despite the FDA’s approval of multiple anti-relapse drugs, relapse rates remain unacceptably high. Furthermore, cognitive deficits among chronic drinkers are evident and are suggested to contribute to relapse risk. Current evidence suggests that several critical features of alcoholism and alcohol-associated neurodegeneration are mechanistically linked to glutamatergic actions; specifically, they appear positively affected by glutamatergic inhibition, particularly inhibition via polyamine modulation of a subpopulation of n-methyl-d-aspartate receptors. The current project was designed to evaluate the performance of two putative polyamine modulators (JR-220 and CP-101,606) in a variety of screens designed …

Applying The Rasch Model To Measure And Compare First- Generation And Continuing-Generation College Students’ Academic Self-Efficacy, Nichole Marie Knutson

Theses and Dissertations--Educational Policy Studies and Evaluation

Students who are the first in their families to attend college are less likely to earn a college degree as compared to their continuing-generation peers. In efforts to increase college graduation rates for first-generation college students, support programs designed to assist first-generation college students are increasing in numbers. These first- generation programs are relying on existing research to build effective curriculums. Even though an extensive body of literature exists in the fields of self-efficacy and first- generation college students, research investigating the self-efficacy of first-generation college students are extremely limited. The research is further limited when examining academic self-efficacy and …

Factors Associated With Tobacco Use Among Rural And Urban Pregnant Women, Whitney Jeanne Katirai

University of Kentucky Doctoral Dissertations

The purpose of this study was to investigate the influences of smoking on rural and urban pregnant women. More specifically, the variables of the knowledge of health effects, health provider recommendations, subscores from the Health Belief Model (HBM), and social support were explored in relation to the smoking behavior of pregnant women. A secondary purpose was to investigate the accuracy of self-reported smoking during pregnancy using biochemical validation. Pregnant women (N=71) completed an anonymous questionnaire, designed by the researcher, to identify variables that predicted smoking for urban and rural women. Participants also gave a saliva sample for cotinine testing.

Approximately …

Mir-17* Suppresses Tumorigenicity Of Prostate Cancer By Inhibiting Mitochondrial Antioxidant Enzymes, Yong Xu, Fang Fang, Jiayou Zhang, Sajni Josson, William H. St. Clair, Daret K. St. Clair

Toxicology and Cancer Biology Faculty Publications

Aberrant micro RNA (miRNA) expression has been implicated in the pathogenesis of cancer. Recent studies have shown that the miR-17-92 cluster is overexpressed in many types of cancer. The oncogenic function of mature miRNAs encoded by the miR-17-92 cluster has been identified from the 5' arm of six precursors. However, the function of the miRNAs produced from the 3' arm of these precursors remains unknown. The present study demonstrates that miR-17* is able to suppress critical primary mitochondrial antioxidant enzymes, such as manganese superoxide dismutase (MnSOD), glutathione peroxidase-2 (GPX2) and thioredoxin reductase-2 (TrxR2). Transfection of miR-17* into prostate cancer PC-3 …

Biomarkers Of Mild Cognitive Impairment And Alzheimer's Disease, Mark A. Lovell, Bert C. Lynn

Chemistry Faculty Patents

A method for quantifying a neurodegenerative disorder in a patient that includes obtaining a fluid sample from the subject; measuring a protein biomarker complex in said fluid sample and correlating the measurement with mild cognitive impairment or Alzheimer's disease status. The biomarkers include those that comprise at least one of a transthyretin protein and/or a prostaglandin-H2 D-isomerase protein, and at least one second, different protein selected from a transthyretin, prostaglandin-H2 D-isomerase, beta-2-microglobulin, cystatin C, superoxide dismutase [Cu—Zn], plasma retinol-binding protein, phosphatidylethanolamine-binding protein, carbonic anhydrase 2, prostaglandin-H2 D-isomerase, and/or serotransferrin protein.

Conformation Dependent Monoclonal Antibodies Distinguish Different Replicating Strains Or Conformers Of Prefibrillar Aβ Oligomers, Rakez Kayed, Isabel Canto, Leonid Breydo, Suhail Rasool, Tamas Lukacsovich, Jessica Wu, Ricardo Albay, Anna Pensalfini, Stephen Yeung, Elizabeth Head, J. Lawrence Marsh, Charles Glabe

Sanders-Brown Center on Aging Faculty Publications

BACKGROUND: Age-related neurodegenerative diseases share a number of important pathological features, such as accumulation of misfolded proteins as amyloid oligomers and fibrils. Recent evidence suggests that soluble amyloid oligomers and not the insoluble amyloid fibrils may represent the primary pathological species of protein aggregates.

RESULTS: We have produced several monoclonal antibodies that specifically recognize prefibrillar oligomers and do not recognize amyloid fibrils, monomer or natively folded proteins. Like the polyclonal antisera, the individual monoclonals recognize generic epitopes that do not depend on a specific linear amino acid sequence, but they display distinct preferences for different subsets of prefibrillar oligomers. Immunological …

Expression Of Functional Bacterial Undecaprenyl Pyrophosphate Synthase In The Yeast Rer2{Delta} Mutant And Cho Cells., Jeffrey S. Rush, Sergey Matveev, Ziqiang Guan, Christian R H Raetz, C J Waechter

Molecular and Cellular Biochemistry Faculty Publications

During evolution the average chain length of polyisoprenoid glycosyl carrier lipids increased from C55 (prokaryotes) to C75 (yeast) to C95 (mammalian cells). In this study, the ability of the E. coli enzyme, undecaprenyl pyrophosphate synthase (UPPS), to complement the loss of the yeast cis-isoprenyltransferase in the rer2Δ mutant was tested to determine if (55)dolichyl phosphate (Dol-P) could functionally substitute in the protein N-glycosylation pathway for (75)Dol-P, the normal isoprenologue synthesized in S. cerevisiae. First, expression of UPPS in the yeast mutant was found to complement the growth and the hypoglycosylation of carboxypeptidase Y defects suggesting that the (55)polyprenyl-P-P intermediate was …

Forage News [2010-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Register Now for Grazing Conference
• Forage Spokesman Contest and KFGC Award Nominations
• PFGC Celebrates 50th Anniversary
• Forage Variety Test Reports
• Feed Costs and Meat Production
• Corn Nitrogen Fertilizer Replacement Value of Alfalfa in Northern Illinois
• Comparison of Seeding Rates and Coating on Seedling Count, Root Length, Root Weight and Shoot Weight of Crimson Clover
• Effect of Macronutrient Deficiency on the Antioxidant Capacity of Lespedeza Cuneata (cv. Au Grazer) and Lotus Corniculatus (cv. Pardee)

Criminal Offending Among Respondents To Protective Orders: Crime Types And Patterns That Predict Victim Risk, Carol E. Jordan, Adam J. Pritchard, Danielle Duckett, Richard Charnigo

Office for Policy Studies on Violence Against Women Publications

Research has shown that respondents to protective orders have robust criminal histories and that criminal offending behavior often follows issuance of a protective order. Nonetheless, the specific nature of the association between protective orders and criminal offending remains unclear. This study uses two classes of statistical models to more clearly delineate that relationship. The models reveal factors and characteristics that appear to be associated with offending and protective order issuance and provide indications about when a victim is most at risk and when the justice system should be most ready to provide immediate protection.