Digital Commons Network^™

Identifying Risk And Protective Factors Of Preparatory And Non-Preparatory Students In Agricultural Education Programs Of A Rural Kentucky High School, Randy J. Adams

Theses and Dissertations--Community & Leadership Development

Today there is an increased demand for secondary agriculture education programs to prepare students to be college and career ready through a program of study. The purpose of this study was to identify risk and protective factors of preparatory and non-preparatory students within an agriculture education program at Anderson County High School in Lawrenceburg, Kentucky. The results of this study did not determine that there was a difference in risk and protective factors between preparatory and non-preparatory students within agriculture education courses. The study concluded that students among both categories were equally exposed to risks such as family conflict and …

Impact Of Transformational Leadership: Exploring The Transformational Leadership Qualities Of Selected Secondary Agriculture Teachers, Ryan H. Thomas

Theses and Dissertations--Community & Leadership Development

The purpose of this study is to determine if transformational leadership styles of agriculture teachers in Jessamine County has an impact on successful transitions of program completers. To be more specific, the focus was to look at agriculture teachers at Jessamine Career and Technology Center (JCTC) to see if they exhibit components of transformational leadership. If so, does transformational leadership of those teachers assist students with successful transitions after graduation? Successful transition is deemed by the Kentucky Department of Education as students that seek post-secondary education, employed full-time or enlist in a branch of armed services.

The population for this …

Analyzing Healthy, Local Food Systems: A Case Study Of Owensboro, Kentucky, Callie Hayden

Theses and Dissertations--Community & Leadership Development

Across the US, support is ever-growing for the local food movement. This growing trend promotes food security, supports farming families, ensures universal access to safe and healthy food products, enhances local economies, and encourages environmental and social well-being. People around the US are implementing sustainable local food systems as a means of meeting their individual community’s needs. While this movement is being popularized dramatically around the world, many cities and regions have yet to address it. This study reviews the existing literature on local food system models in an effort to answer the research question: What are the “ingredients” of …

Investigation Of Media Ingredients And Water Sources For Algae Co2 Capture At Different Scales To Demonstrate The Correlations Between Lab-Scale And Large-Scale Growth, Tabitha Graham

Theses and Dissertations--Biosystems and Agricultural Engineering

As energy use increases globally the environmental burdens increase alike. Many accusations have been made that carbon dioxide is a culprit of climate change. The University of Kentucky and Duke Energy Power have partnered to test carbon capture technology in a large scale project. To this end, the objective of this thesis is to investigate potential water media sources and nutrient sources at different volume scales for algae cultivation to help create a more environmentally viable and economically feasible solution. This work will conduct a life cycle assessment of water media sources and the effects of the inputs and outputs …

Alkaline Hydrogen Peroxide Pretreatment For Its Use In An On-Farm Bioprocessing Facility, Mary Kathryn Gray

Theses and Dissertations--Biosystems and Agricultural Engineering

Pretreatment is an essential step in biofuel production from lignocellulose. Disruption of the lignin structure gives enzymes and fermentation organisms access to long chains of cellulose and hemicellulose. For this project’s purposes, the pretreatment must work within the framework of an on-farm butanol bioprocessing facility. Alkaline hydrogen peroxide (AHP) is a delignification method that potentially provides several advantages. At the alkaline pH, powerful hydroxyl radicals are formed; which attack lignin. The objectives of this study were to determine if AHP removes substantial lignin for the feedstocks, corn stover, wheat straw, switchgrass and miscanthus, and to determine if AHP acts as …

Hydrologic Characterization Of A Rain Garden Mitigating Stormwater Runoff From A Commercial Area, John T. Mcmaine

Theses and Dissertations--Biosystems and Agricultural Engineering

Impervious surfaces such as roads, sidewalks, and roofs increase the volume of runoff generated in a watershed. Traditional stormwater management techniques emphasize conveyance of runoff away from impervious surfaces in order to reduce flooding. Rain gardens are becoming popular as a different means to manage stormwater in such a way that runoff is captured and infiltrated onsite rather than conveyed offsite. A stormwater management system consisting of a rainwater harvest system, rain garden, and infiltration chamber was built at the Coca-Cola Refreshments USA, Inc. distribution center in Lexington, Kentucky during the fall of 2011. Precipitation, inflow, and water level were …

Effects Of Intranasally Administered Dnsp-11 On The Central Dopamine System Of Normal And Parkinsonian Fischer 344 Rats, James H. Sonne

Theses and Dissertations--Neuroscience

Due to the blood-brain barrier, delivery of many drugs to the brain has required intracranial surgery which is prone to complication. Here we show that Dopamine Neuron Stimulating Peptide 11 (DNSP-11), following non-invasive intranasal administration, protects dopaminergic neurons from a lesion model of Parkinson’s disease in the rat. A significant and dose-dependent increase in an index of dopamine turnover (the ratio of DOPAC to dopamine) was observed in the striatum of normal young adult Fischer 344 rats by whole-tissue neurochemistry compared to vehicle administered controls.

Among animals challenged with a moderate, unilateral 6-hydroxy-dopamine (6-OHDA) lesion of the substantia nigra, those …

Molecular And Cellular Characterization Of Dopamine Neuron Stimulating Peptides, Kristen Kelps

Theses and Dissertations--Neuroscience

Parkinson’s disease, the second most common neurodegenerative disease, is characterized by the loss of dopaminergic neurons within the substantia nigra. Currently, the treatments available for PD are symptomatic treatments that do not stop the progression of the disease. Trophic molecules, such as glial cell-line derived neurotrophic factor (GDNF), have been evaluated as potential therapeutic molecules that could stop the loss of neurons and potentially restore some of the neurons that have already been lost. However, these trophic molecules are large making them difficult to produce and delivery. Here we characterize three peptides (DNSP-5, DNSP-11, and DNSP-17) to determine it they …

Molecular Mechanisms By Which C-Abl And Arg Mediate Melanoma Invasion And Metastasis, Sourik S. Ganguly

Theses and Dissertations--Pharmacology and Nutritional Sciences

Metastasis is one of the main causes of death in cancer patients. Metastatic melanoma is a death sentence, as chemotherapeutic agents have a 5% success rate or do not extend survival beyond 10 months. The lack of effective chemotherapeutic agents for treating metastatic melanoma indicates a dire need to identify new drug targets and develop new therapies. Our lab has previously shown that the kinase activity of Abelson family of non-receptor tyrosine kinases (c-Abl and Arg) is elevated in invasive breast cancer cell lines as compared to non-invasive cell lines. Previous studies from our lab have shown that Abl …

Changes In Sleep Architecture And Cognition With Age And Psychosocial Stress: A Study In Fischer 344 Rats, Heather M. Buechel

Theses and Dissertations--Pharmacology and Nutritional Sciences

Changes in both sleep architecture and cognition are common with age. Typically these changes have a negative connotation: sleep fragmentation, insomnia, and deep sleep loss as well as forgetfulness, lack of focus, and even dementia and Alzheimer’s disease. Research has shown that psychosocial stressors, such as isolation from family and friends or loss of a loved one can also have significant negative effects on sleep architecture and cognitive capabilities. This leaves the elderly in a particularly vulnerable situation: suffering from cognitive decline and sleep dysregulation already, and more likely to respond negatively to psychosocial stressors. Taking all of these factors …

Inhibition Of Calpains By Calpastatin: Implications For Cellular And Functional Damage Following Traumatic Brain Injury, Kathleen M. Schoch

Theses and Dissertations--Physiology

Traumatic brain injury (TBI) is a devastating health problem based on its high incidence, economic burden, and lack of effective pharmacological treatment. Individuals who suffer an injury often experience lifelong disability. TBI results in abrupt, initial cell damage leading to delayed neuronal death. The calcium-activated proteases, calpains, are known to contribute to this secondary neurodegenerative cascade. Prolonged activation of calpains results in proteolysis of numerous cellular substrates including cytoskeletal components, membrane receptors, and cytosolic proteins, contributing to cell demise despite coincident expression of calpastatin, the specific inhibitor of calpains.

A comprehensive analysis using two separate calpastatin transgenic mouse lines was …

Sex Differences In Cell Death And Steroid Hormone Receptors In Cortical Explants, Amanda L. Trout

Theses and Dissertations--Physiology

Estrogens, such as the biologically active 17-b estradiol (E2) have many actions in the male and female brain. Not only does E2 regulate reproductive behavior in adults, it organizes and activates the brains of younger animals in a sex-specific manner. In addition, many human studies have shown E2 to provide protection against a variety of neurological disorders, including stoke. These studies have been controversial and depend largely on the type and timing of hormone replacement. Animal studies are much less controversial and clearly demonstrate a neuroprotective role for E2 following ischemic brain injury. Because much of E2 neuroprotection requires sex …

Mechanistic Basis For Atrial And Ventricular Arrhythmias Caused By Kcnq1 Mutations, Daniel C. Bartos

Theses and Dissertations--Physiology

Cardiac arrhythmias are caused by a disruption of the normal initiation or propagation of electrical impulses in the heart. Hundreds of mutations in genes encoding ion channels or ion channel regulatory proteins are linked to congenital arrhythmia syndromes that increase the risk for sudden cardiac death. This dissertation focuses on how mutations in a gene (KCNQ1) that encodes a voltage-gated K⁺ ion channel (Kv7.1) can disrupt proper channel function and lead to abnormal repolarization of atrial and ventricular cardiomyocytes.

In the heart, Kv7.1 coassembles with a regulatory protein to conduct the slowly activating delayed rectifier K^{+ …}

Evaluation Of Insulin-Like Growth Factor-1 As A Therapeutic Approach For The Treatment Of Traumatic Brain Injury, Shaun W. Carlson

Theses and Dissertations--Physiology

Traumatic brain injury (TBI) is a prevalent CNS neurodegenerative condition that results in lasting neurological dysfunction, including potentially debilitating cognitive impairments. Despite the advancements in understanding the complex damage that can culminate in cellular dysfunction and loss, no therapeutic treatment has been effective in clinical trials, highlighting that new approaches are desperately needed. A therapy that limits cell death while simultaneously promoting reparative mechanisms, including post-traumatic neurogenesis, in the injured brain may have maximum effectiveness in improving recovery of function after TBI. Insulin-like growth factor-1 (IGF-1) is a potent growth factor that has previously been shown to promote recovery of …

A Tale Of Two Envys: A Social Network Perspective On The Consequences Of Workplace Social Comparison, Christopher M. Sterling

Theses and Dissertations--Management

My dissertation examines how individuals respond to workplace social comparisons. I measure the explicit set of referent others that individuals compare themselves against in order to evaluate their own level of performance. I examine how the social context of these comparisons impact discretionary performance related behaviors by examining how an individual’s position within a social network and the structural characteristics of an individual’s reference group influences the experience of discrete emotions. Specifically, I examine how malicious envy and benign envy mediate the relationship between social comparison and workplace behavior in a field setting. Results indicate that social network structure plays …

Employment Of Single Mothers: Child Care Costs And The Effectiveness Of The Eitc, Salma Begum

Theses and Dissertations--Economics

This dissertation examines the effect of the Earned Income Tax Credit (EITC) on labor force participation of single mothers by controlling for child care costs. Based on a simple model of utility maximizing households that jointly determine hours worked and hours of non-maternal child care demanded, I estimate the change in the labor force participation rate of single mothers following the EITC expansions of the 1990s. In order to investigate the usage of different modes of childcare services, a multinomial logit model has been estimated. The data source for the study is topical module panels of the Survey of Income …

Two Essays On Housing: Using Hedonic And Quasi-Experimental Methods In (Dis)Amenity Valuation With Housing Data: The Case Of Communication Antennas, And The Value Of Brand Name Franchises Compared To Local Real Estate Brokerage Firms, Stephen L. Locke

Theses and Dissertations--Economics

This dissertation consists of two essays on housing, the first on estimation strategies for the valuation of a local disamenity and the second on the structure of the market for the services of real estate brokers.

The purpose of the first essay is to apply hedonic and quasi-experimental methods to measure the value of any disamenity caused by communication antennas. Crucial to unbiased estimates is accounting for both endogenous antenna location and changes in unobservable housing and neighborhood characteristics. Spatial fixed effects are used to control for unobservable characteristics that can influence the location decisions of residents and the location …

Strategic Responses To Tax And Transfer Policy: Welfare Competition, Tax Competition And The Elasticity Of Taxable Income, Sarah K. Burns

Theses and Dissertations--Economics

My dissertation consists of three essays focused on identifying the strategic responses of governments and individuals following changes in the tax and transfer system. Two essays contribute to the literature on fiscal competition, focusing on state level polices aimed at redistributing income. A third essay contributes to the literature estimating the responsiveness of individual’s incomes to changing marginal tax rates. A better understanding of these responses contributes to our ability to design an optimal tax and transfer system in a federalist nation.

In essay 1 I employ a spatial dynamic approach to investigate interstate welfare competition across multiple policy instruments …

Glutamate Dysregulation And Hippocampal Dysfunction In Epileptogenesis, Seth R. Batten

Theses and Dissertations--Medical Sciences

Epileptogenesis is the complex process of the brain developing epileptic acitivity. Due to the role of glutamate and the hippocampus in synaptic plasticity a dysregulation in glutamate neurotransmission and hippocampal dysfunction are implicated in the process of epileptogenesis. However, the exact causal factors that promote epileptogenesis are unknown.

We study presynaptic proteins that regulate glutamate neurotransmission and their role in epileptogenesis. The presynaptic protein, tomosyn, is believed to be a negative regulator of glutamate neurotransmission; however, no one has studied the effects of this protein on glutamate transmission in vivo. Furthermore, evidence suggests that mice lacking tomosyn have a …

Peacekeeping And Peace Kept: Third Party Interventions And Recurrences Of Civil War, Barrett J. Osborn

Theses and Dissertations--Political Science

Civil wars have become more prevalent in modern times and present unique challenges to conflict resolution. Third parties often intervene in civil wars attempting to insure that peace is imposed and will persist. However, the impact of third parties on intrastate conflicts remains incomplete. The civil conflict literature does not sufficiently distinguish how third parties promote peaceful outcomes during a peacekeeping operation and why a state remains stable after the peacekeepers leave. By examining data on third party interventions from 1946-2006 and individually examining the case of Sierra Leone, this research concludes that peacekeeping missions promoting transparency, credible information sharing, …

Conviction Celerity, Punishment Severity, And Treatment Compliance As Predictors Of Dui Recidivism: Mediation And Moderation Models Of Deterrence, Megan F. Dickson

Theses and Dissertations--Sociology

Driving under the influence (DUI) is one of the most frequently committed offenses in the United States and approximately one-third of DUI offenders are recidivists. Researchers have evaluated multiple DUI prevention approaches, most of which have been rooted in deterrence theory. Recently, the criminal justice system has moved away from deterrence-based approaches and begun employing various forms of rehabilitation to reduce DUI recidivism. This shift in the criminal justice system has lead researchers to begin exploring the effects of rehabilitation on DUI offenders, including an examination of offender compliance with rehabilitation programs. Although each of these areas has been investigated …

High-Activity Mutants Of Human Butyrylcholinesterase For Cocaine Abuse Treatment, Liu Xue

Theses and Dissertations--Pharmacy

Cocaine is a widely abused drug without an FDA-approved medication. It has been recognized as an ideal anti-cocaine medication to accelerate cocaine metabolism producing biologically inactive metabolites via a route similar to the primary cocaine-metabolizing pathway, i.e. butyrylcholinesterase (BChE)-catalyzed hydrolysis. However, the native BChE has a low catalytic activity against cocaine. We recently designed and discovered a set of BChE mutants with a high catalytic activity specifically for cocaine. An ideal, therapeutically valuable mutant of human BChE should have not only a significantly improved catalytic activity against cocaine, but also certain selectivity for cocaine over neurotransmitter acetylcholine (ACh) such …

Computational Modeling, Design, And Characterization Of Cocaine-Metabolizing Enzymes For Anti-Cocaine Medication, Lei Fang

Theses and Dissertations--Pharmacy

Cocaine is a widely abused and addictive drug, resulting in serious medical and social problems in modern society. Currently, there is no FDA-approved medication specific for cocaine abuse treatment. The disastrous medical and social consequences of cocaine abuse have made the development of an anti-cocaine medication a high priority. However, despite decades of efforts, traditional pharmacodynamic approach has failed to yield a truly useful small-molecule drug due to the difficulties inherent in blocking a blocker like cocaine without affecting the normal functions of the transporters or receptors. An alternative approach, i.e. pharmacokinetic approach, is to interfere with the delivery of …

Multistate Markov Chains And Their Application To The Biologically Resilient Adults In Neurological Studies Cohort, Erin L. Abner

Theses and Dissertations--Epidemiology and Biostatistics

Dementia is increasingly recognized as a major and growing threat to public health worldwide, and there is a critical need for prevention and treatment strategies. However, it is necessary that appropriate methodologies are used in the identification of risk factors. The purpose of this dissertation research was to develop further the body of literature featuring Markov chains as an analytic tool for data derived from longitudinal studies of aging and dementia.

Data drawn from 649 participants in the University of Kentucky’s Alzheimer’s Disease Center’s (UK ADC) Biologically Resilient Adults in Neurological Studies (BRAiNS) cohort, which was established in 1989 and …

Assessing Self-Efficacy Of Cultural Competence With Lesbian, Gay, And Bisexual Clients: A Comparison Of Training Methods With Graduate Social Work Students, Steven D. Johnson

Theses and Dissertations--Social Work

Graduate social work students are mandated to be cultural competent to work with lesbian, gay, and bisexual (LGB) clients. This exploratory study examined how best to teach graduate social work students to be culturally competent in working with LGB clients by assessing their perceived competence of attitudes, knowledge and skills as well as their demonstrated competence through case vignettes.

The study compared a current pedagogical method of infusing LGB material across the curricula with two types of brief trainings (didactic lecture and experiential) . This mixed methods study utilized a pretest/posttest design to examine the effects of the trainings as …

Factors Affecting End Of Treatment Symptom Severity For Children Receiving Trauma-Informed Evidence-Based Treatment, Jessica G. Eslinger

Theses and Dissertations--Social Work

The purpose of this project is to examine how the factors of gender, placement status, type of treatment, the number of different types of trauma experienced, and a child’s age at the start of treatment may influence end of treatment symptom severity scores for children ages 2-12 years who received trauma-informed evidence-based treatment for trauma. Method: Caregivers and children receiving outpatient services (N=134) completed the Child Behavioral Checklist, Trauma Symptom Checklist for Young Children, and the Trauma Symptom Checklist for Children-Alternate Version at baseline and end of treatment. Hypotheses were tested with a series of ANCOVA analyses, Independent t-Tests, …

Acculturative Stress And Depressive Symptoms Among Korean Immigrant Elders Residing In Non-Korean Ethnic Enclaves, Stephanie Lyu Rhee

Theses and Dissertations--Social Work

Few studies have examined the relationships among personal factors, acculturative stress, coping resources, and depression of Korean immigrant elders residing in areas without any Korean ethnic enclave. Based on the stress and coping model and the sociocultural model of stress, coping, and adaptation, this cross-sectional study examined the relationships among acculturative stress, coping, and depression in 111 non-institutionalized Korean immigrant elders aged 60 and older residing in areas without any Korean ethnic enclaves in three neighboring states of Southwestern Ohio, North Central Region of Kentucky, and Southern Indiana. A majority of convenience and snowball sample participated in self-administered mailed surveys, …

Identity Making Process Of Individuals With Mild Intellectual Disabilities, Carolee Kamlager

Theses and Dissertations--Social Work

The shadow of stigma theory typically surrounds the research investigation of the lives of individuals with mild intellectual disabilities. McAdams’ life story theory and methodology provide a human development framework as an alternative to the prevailing framework in the field of disability. This study moves out of the shadow of otherness and examines the personal identity making process of twelve individuals with mild intellectual disabilities in the light of human development theory. Findings dispel the assumption that individuals with mild intellectual disabilities construct their lives solely through their disability. Rather, the identity making process includes the influences of socio-cultural events, …

Characterizing The Variability In Respirable Dust Exposure Using Johnson Transformation And Re-Examining 2010 Proposed Changes To The U.S. Underground Coal Mine Dust Standard, Al I. Khan

Theses and Dissertations--Mining Engineering

Coal workers’ pneumoconiosis (CWP), commonly referred to as black lung, is a chronic lung disease that results from the inhalation and deposition of coal dust in the lungs. While this disease continues to afflict coal miners, its prevalence has steadily declined over three decades since 1970. Based on a voluntary X-ray surveillance program, conducted by the National Institute for Occupational Safety and Health (NIOSH), this downward trend, however, ended in 2000 and has actually begun to rise. The Mine Safety and Health Administration (MSHA) instituted a Comprehensive Initiative to “End Black Lung” to combat the reported upturn in black …

Alterations In Visual Processing And Its Impact On Upright Postural Stability In Athletes Following Sport-Related Concussion, Andrea E. Cripps

Theses and Dissertations--Rehabilitation Sciences

Athletes are at risk of sustaining a concussion in all sports and at all competitive levels which may lead to balance impairments. Balance results from the integration of visual, vestibular, and somatosensory information. The underlying pathophysiology for balance impairments is not well understood and visuo-motor processing impairments and how these impairments contribute to balance in concussed athletes has not been reported. Objectives: (1) to investigate the influence of visual perturbation on upright postural stability and balance in athletes who have recently suffered a sports-related concussion, (2) to establish the test-retest reliability of a simple visuo-motor processing task. Design: A longitudinal, …