Digital Commons Network^™

Building The Science Of Delivery: Public Health Services & Systems Research, Glen P. Mays

Health Management and Policy Presentations

Studying variation and innovation in public health delivery can identify pathways for improving the organization, financing, and implementation of programs and policies that protect and promote health.

An Orange Fluorescent Protein Tagging System For Real-Time Pollen Tracking, J. Hollis Rice, Reginald J. Millwood, Richard E. Mundell, Orlando D. Chambers, Laura L. Abercrombie, H. Maelor Davies, C. Neal Stewart

Kentucky Tobacco Research and Development Center Faculty Publications

BACKGROUND: Monitoring gene flow could be important for future transgenic crops, such as those producing plant-made-pharmaceuticals (PMPs) in open field production. A Nicotiana hybrid (Nicotiana. tabacum x Nicotiana glauca) shows limited male fertility and could be used as a bioconfined PMP platform. Effective assessment of gene flow from these plants is augmented with methods that utilize fluorescent proteins for transgenic pollen identification.

RESULTS: We report the generation of a pollen tagging system utilizing an orange fluorescent protein to monitor pollen flow and as a visual assessment of transgene zygosity of the parent plant. This system was created to generate a …

Arizona Public Libraries Serving The Spanish-Speaking: Context For Changes, Denice Adkins, C. Sean Burns

Information Science Faculty Publications

Arizona is at the forefront of Latino population growth and political and racial politics. Three different factors could potentially influence the provision of library service to Latinos in the State of Arizona. These are (1) the growth of the Latino community and the consequent growth of its library needs, (2) the growth of state legislation that is hostile to immigrants and Latinos, and (3) the promotion of a pro-immigrant position by the library profession. This paper compares services to the Spanish-speaking in the State of Arizona from 1999 to 2009 in light of conflicting pro- and anti-immigrant sentiments operating in …

Proteomic Analysis Of Mismatch Repair-Mediated Alkylating Agent-Induced Dna Damage Response, Xi Chen, Yong Zhao, Guo-Min Li, Lin Guo

Toxicology and Cancer Biology Faculty Publications

BACKGROUND: Mediating DNA damage-induced apoptosis is an important genome-maintenance function of the mismatch repair (MMR) system. Defects in MMR not only cause carcinogenesis, but also render cancer cells highly resistant to chemotherapeutics, including alkylating agents. To understand the mechanisms of MMR-mediated apoptosis and MMR-deficiency-caused drug resistance, we analyze a model alkylating agent (N-methyl-N’-nitro-N-nitrosoguanidine, MNNG)-induced changes in protein phosphorylation and abundance in two cell lines, the MMR-proficient TK6 and its derivative MMR-deficient MT1.

RESULTS: Under an experimental condition that MNNG-induced apoptosis was only observed in MutSα-proficient (TK6), but not in MutSα-deficient (MT1) cells, quantitative analysis …

A Content Analysis Of Media Coverage Of The Introduction Of A Smoke-Free Bylaw In Vancouver Parks And Beaches, Arezu Moshrefzadeh, Wendy Rice, Ann Pederson, Chizimuzo T.C. Okoli

Nursing Faculty Publications

The Board of Parks and Recreation in Vancouver, BC approved a smoke-free bylaw in the city's parks, beaches and recreational facilities, effective 1 September 2010. We analyzed local news coverage and portrayal of the bylaw to understand the potential influence of news media on public perception of the bylaw in order to inform the media advocacy work of public health interest groups. We compiled a data set of newspaper articles (n = 90) and conducted a quantitative content analysis to examine content related to the outdoor smoke-free policy, including article slant, topics related to smoking and tobacco control, and any …

The Ral Exchange Factor Rgl2 Promotes Cardiomyocyte Survival And Inhibits Cardiac Fibrosis, Rebecca L. Scotland, Leah Allen, Leah J. Hennings, Ginell R. Post, Steven R. Post

Pharmacology and Nutritional Sciences Faculty Publications

Cardiomyocytes compensate to acute cardiac stress by increasing in size and contractile function. However, prolonged stress leads to a decompensated response characterized by cardiomyocyte death, tissue fibrosis and loss of cardiac function. Identifying approaches to inhibit this transition to a decompensated response may reveal important targets for treating heart failure. The Ral guanine nucleotide disassociation (RalGDS) proteins are Ras-interacting proteins that are upregulated by hypertrophic stimuli. The Ral guanine nucleotide dissociation stimulator-like 2 (Rgl2) is a member of the RalGDS family that modulates expression of hypertrophic genes in cardiomyocytes. However, the pathophysiologic consequence of increased Rgl2 expression in cardiomyoctyes remains …

Adolescence, Violence, And Public Health, Joav Merrick, Isack Kandel, Hatim A. Omar

Pediatrics Faculty Publications

No abstract provided.

Accreditation And Standardization In Local Public Health, Glen P. Mays

Health Management and Policy Presentations

Reducing harmful, wasteful, and inequitable variation in public health delivery is a central objective for administrative and policy decision-makers in public health, and is embedded in the national accreditation movement. The challenge lies in accomplishing this objective while preserving the benefits of customization in public health delivery that accrue through tailoring and targeting of resources to unique community needs and risks. Public health services and systems research (PHSSR) can help determine the optimal balance between standardization and customization in public health delivery.

Dreadds In Drosophila: A Pharmacogenetic Approach For Controlling Behavior, Neuronal Signaling, And Physiology In The Fly, Jaime Becnel, Oralee Johnson, Zana R Majeed, Vi Tran, Bangning Yu, Bryan L. Roth, Robin L. Cooper, Edmund K. Kerut, Charles D. Nichols

Biology Faculty Publications

We have translated a powerful genetic tool, designer receptors exclusively activated by designer drugs (DREADDs), from mammalian systems to Drosophila melanogaster to selectively, rapidly, reversibly, and dose-dependently control behaviors and physiological processes in the fly. DREADDs are muscarinic acetylcholine G protein-coupled receptors evolved for loss of affinity to acetylcholine and for the ability to be fully activated by an otherwise biologically inert chemical, clozapine-N-oxide. We demonstrate its ability to control a variety of behaviors and processes in larvae and adults, including heart rate, sensory processing, diurnal behavior, learning and memory, and courtship. The advantages of this particular technology include the …

Strengthening The Evidence Base For Accreditation: Progress In Phssr And Pbrns, Glen P. Mays

Health Management and Policy Presentations

With the launch of a voluntary national accreditation program for public health agencies, the field requires an expanding evidence base concerning which public health strategies work best, for which populations, and under what circumstances. The field of public health services and systems research (PHSSR) and practice-based research networks (PBRNs) are producing applied studies that can inform the future development and implementation of accreditation standards, and evaluate the impact of accreditation on the field of practice.

The Flexible Fairness: Equality, Earned Entitlement, And Self-Interest, Chunliang Feng, Yi Luo, Ruolei Gu, Lucas S. Broster, Xueyi Shen, Tengxiang Tian, Yue-Jia Luo, Frank Krueger

Behavioral Science Faculty Publications

The current study explored whether earned entitlement modulated the perception of fairness in three experiments. A preliminary resource earning task was added before players decided how to allocate the resource they jointly earned. Participants' decision in allocation, their responses to equal or unequal offers, whether advantageous or disadvantageous, and subjective ratings of fairness were all assessed in the current study. Behavioral results revealed that participants proposed more generous offers and showed enhanced tolerance to disadvantageous unequal offers from others when they performed worse than their presumed "partners," while the reverse was true in the better-performance condition. The subjective ratings also …

Psmb9 Codon 60 Polymorphisms Have No Impact On The Activity Of The Immunoproteasome Catalytic Subunit B1i Expressed In Multiple Types Of Solid Cancer, Ji Eun Park, Lin Ao, Zachary Miller, Kyungbo Kim, Ying Wu, Eun Ryoung Jang, Eun Young Lee, Kyung Bo Kim, Wooin Lee

Pharmaceutical Sciences Faculty Publications

The proteasome is a key regulator of cellular protein homeostasis and is a clinically validated anticancer target. The immunoproteasome, a subtype of proteasome expressed mainly in hematopoietic cells, was initially recognized for its role in antigen presentation during the immune response. Recently, the immunoproteasome has been implicated in several disease conditions including cancer and autoimmune disorders, but many of the factors contributing to these pathological processes remain unknown. In particular, the codon 60 polymorphism of the PSMB9 gene encoding the β1i immunoproteasome catalytic subunit has been investigated in the context of a variety of diseases. Despite this, previous studies have …

2012 Kentucky Equine Survey, College Of Agriculture, Food And Environment, University Of Kentucky, Kentucky Agricultural Development Fund, Kentucky Horse Council, Ag Equine Programs, University Of Kentucky

Kentucky Equine Survey

The last thorough equine inventory in Kentucky was undertaken in 1977, leaving the state’s equine industry with 35 years of changes, growth and con­traction that were neither measured nor analyzed.

In response to these needs, in 2011, the University of Kentucky and the Kentucky Horse Council, with support by industry organizations spearheaded by the Kentucky Quarter Horse Association, the Kentucky Thoroughbred Farm Managers’ Club and Kentucky Thoroughbred Association/Kentucky Thoroughbred Owners and Breeders, and initial support from the University of Louisville, succeeded in securing fund­ing for a large-scale, comprehensive equine survey upon which future industry plans could be built. This was …

Molecular Signatures In Urologic Tumors, Spencer Larkin, Natasha Kyprianou

Surgery Faculty Publications

Urologic tumors continue to represent a huge fraction of cancer cases in the United States, with over 376,310 estimated new diagnoses in 2013. As with many types of tumors, urologic tumors vary greatly in their phenotype, ranging from minimally invasive to malignancies possessing great metastatic potential. The increasing need for more efficient and less invasive methods of cancer detection, as well as the ability to predict severity of the disease phenotype is readily evident--yet reliable methods remain elusive in a clinical setting today. Comprehensive panels of gene clusters are being developed toward the generation of molecular signatures in order to …

Public Health Practice-Based Research Networks: Creating Evidence For Practice, Glen P. Mays

Health Management and Policy Presentations

Powerful opportunities for learning from variation and change in public health delivery now exists across the U.S. health system. Practice-based research networks (PBRNs) provide the vehicles for harvesting evidence from applied, comparative studies -- and for feeding this information back into the delivery system.

Public Health Services & Systems Research: Concepts, Methods, And Emerging Findings, Glen P. Mays

Health Management and Policy Presentations

The field of public health services & systems research (PHSSR) increasingly is addressing the question of how to assess the value of investments in public health programs, infrastructure, and delivery systems. Progress in quasi-experimental research designs, measurement, estimation techniques, and data sources are yielding important insight.

Why Are So Many Americans On Food Stamps? The Role Of The Economy, Policy, And Demographics, James P. Ziliak

University of Kentucky Center for Poverty Research Discussion Paper Series

1 in 7 Americans received assistance from SNAP in FY2012, which is a rate 141 percent higher than in FY2000, but only 59 percent higher than in FY1980. In this chapter I describe the socioeconomic and policy climate in recent decades that had bearing on SNAP participation, along with a formal empirical analysis of those determinants and detailed simulations of the relative contributions of the economy, policy, and demographics to changes in SNAP participation over time. The results suggest that SNAP is operating effectively as an automatic fiscal stabilizer—nearly 50 percent of the increase in participation from 2007-2011 is due …

Finding Common Ground: Defining And Measuring Provider-Patient Rapport, Gretchen Norling Holmes

Center of Excellence in Rural Health Presentations

Rapport, an important factor in provider-patient communication and a key component of relational communication, has been inconsistently defined in the literature. Additionally, researchers have found it difficult to measure to its complex and often ambiguous nature. This project provides a conceptual and operational definition of rapport, as well, as identifies some of the measurable components of rapport.

A Diffusible Mobile Dental Services Model For Prevention-Focused Outreach For Underserved Children In Rural Communities, Nikki Stone, Frances J. Feltner, Willie Bates

Center of Excellence in Rural Health Presentations

Background: In 2006 a mobile dental outreach program began operating full-time at the UK North Fork Valley Community Health Center in Hazard, Kentucky, a federally-qualified health center administered by the University of Kentucky and located at the UK Center of Excellence in Rural Health. This program continues to provide preventive dental care to children at forty different elementary schools and Head Start centers in a four county area of southeastern Kentucky located in the heart of rural Appalachia. The counties are Leslie, Knott, Letcher and Perry (LKLP), which are part of the Kentucky River Development District and encompass the LKLP …

Kentucky’S Growing Need For Medical Laboratory Practitioners, Michelle Butina, Frances J. Feltner

Center of Excellence in Rural Health Presentations

Context: The U.S. Bureau of Labor Statistics predicts a 15% growth in medical laboratory science (MLS, formerly clinical laboratory science and medical technology) graduates from 2010 to 2020. Kentucky is underserved and has a maldistribution of medical laboratory practitioners, composed of scientists and technicians, with rural communities more likely to be underserved by MLS graduates. 1 Implementation of the Affordable Care Act (ACA) is likely to increase demand for medical laboratory practitioners because of the ACA’s preventative health focus and the expansion of access to healthcare. An increase in the aging population will also contribute to a greater need to …

Pharmacy Law Brief: National Practitioner Data Bank - What Is It?, Joseph L. Fink Iii

Pharmacy Practice and Science Faculty Publications

No abstract provided.

Enzymatic Hydrolysis Of Biomass At High-Solids Loadings – A Review, Alicia A. Modenbach, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

Enzymatic hydrolysis is the unit operation in the lignocellulose conversion process that utilizes enzymes to depolymerize lignocellulosic biomass. The saccharide components released are the feedstock for fermentation. When performed at high-solids loadings (≥ 15% solids, w/w), enzymatic hydrolysis potentially offers many advantages over conversions performed at low- or moderate-solids loadings, including increased sugar and ethanol concentrations and decreased capital and operating costs.

The goal of this review is to provide a consolidated source of information on studies using high-solids loadings in enzymatic hydrolysis. Included in this review is a brief discussion of the limitations, such as a lack of available …

The Influence Of Parental Aspirations, Attitudes, And Engagement On Children's Very Low Food Security, Elizabeth T. Powers

University of Kentucky Center for Poverty Research Discussion Paper Series

Survey of Income and Program Participation data are used to investigate the relationship between parenting and children’s very low food security. Parenting is characterized along five domains (emotional outlook, support, education desires, activities with the child excluding meals, and television viewing rules). Food security definitions are obtained from questions in a special SIPP module that are based on the USDA’s core food security module. Graphical evidence indicates that parenting patterns differ distinctly for households experiencing various levels of food insecurity. Descriptive regression evidence suggests that some of the parenting attributes are significantly associated with children’s food insecurity, even controlling for …

Risk And Protective Factors Associated With Prevalence Of Vlfs In Children Among Children Of Foreign-Born Mothers, John Cook

University of Kentucky Center for Poverty Research Discussion Paper Series

This research examined VLFS in children among households with foreign-born (FB) mothers compared to US-born mothers through three research questions: Is mother’s foreign-born status (FBS) associated with VLFS in children, and can association be explained by mothers’ socio-demographic characteristics? Are FB mothers more or less likely to receive nutrition or non-nutrition assistance benefits, or work for pay than US-born mothers? Do mothers’ FBS, or protective/risk factors associated with FBS, modify associations of negative economic shocks and hardships with VLFS in children? Data are on approximately 44,000 mother-child (ages<48 Mos.) dyads collected from household surveys administered under a "sentinel surveillance" system over 1998-2012 at teaching hospitals and clinics in seven US cities. Bivariate and multivariate logistic regression models tested study hypotheses. Mothers' FBS is strongly positively associated with VLFS in children after controlling for available risk and protective factors. FB mothers are less likely to receive SNAP and non-nutrition assistance (TANF, LIHEAP or housing subsidies), but more likely to receive WIC and to be employed than US-born mothers. FB mothers are no more likely to report negative reasons for not receiving SNAP or TANF, or losing jobs or decreasing work hours than US-born, and reported "immigration concerns" rarely. No need/chose not to participate are most frequently reported reasons for not receiving SNAP and TANF; pregnancy/maternity leave and "market conditions" for lost jobs and decreased work hours. Economic shocks and hardships are positively associated with VLFS in children, but Mothers' FBS does not interact with shocks and hardships to modify those associations.

Food Insecurity During Childhood: Understanding Persistence And Change Using Linked Current Population Survey Data, Sheela Kennedy, Catherine A. Fitch, John Robert Warren, Julia A. Rivera Drew

University of Kentucky Center for Poverty Research Discussion Paper Series

Our paper examines the prevalence and determinants of children’s transitions into and out of food insecurity since 2001. We use longitudinally linked data from the Food Security Supplements to the Current Population Surveys to estimate one-year transition probabilities of entry and exit from food insecurity. Our results indicate that child hunger is typically short-lived, but children experiencing very low food security frequently experience multiple consecutive years of food insecurity. We demonstrate large demographic and socioeconomic differences in rates of entry into very low food security and persistence in children's food insecurity. Income and employment shocks are important predictors of child …

Circulating Micrornas In Alzheimer's Disease: The Search For Novel Biomarkers, Véronique Dorval, Peter T. Nelson, Sébastien S. Hébert

Sanders-Brown Center on Aging Faculty Publications

Alzheimer's disease (AD) is the most common neurodegenerative disease in the elderly. While advancements have been made in understanding the genetic and molecular basis of AD, the clinical diagnosis of AD remains difficult, and post-mortem confirmation is often required. Furthermore, the onset of neurodegeneration precedes clinical symptoms by approximately a decade. Consequently, there is a crucial need for an early and accurate diagnosis of AD, which can potentially lead to strategies that can slow down or stop the progression of neurodegeneration and dementia. Recent advances in the non-coding RNA field have shown that microRNAs (miRNAs) can function as powerful biomarkers …

Reproducibility Of Cine Displacement Encoding With Stimulated Echoes (Dense) Cardiovascular Magnetic Resonance For Measuring Left Ventricular Strains, Torsion, And Synchrony In Mice, Christopher M. Haggerty, Sage P. Kramer, Cassi M. Binkley, David K. Powell, Andrea C. Mattingly, Richard Charnigo, Frederick H. Epstein, Brandon K. Fornwalt

Pediatrics Faculty Publications

BACKGROUND: Advanced measures of cardiac function are increasingly important to clinical assessment due to their superior diagnostic and predictive capabilities. Cine DENSE cardiovascular magnetic resonance (CMR) is ideal for quantifying advanced measures of cardiac function based on its high spatial resolution and streamlined post-processing. While many studies have utilized cine DENSE in both humans and small-animal models, the inter-test and inter-observer reproducibility for quantification of advanced cardiac function in mice has not been evaluated. This represents a critical knowledge gap for both understanding the capabilities of this technique and for the design of future experiments. We hypothesized that cine DENSE …

Tricellulin Deficiency Affects Tight Junction Architecture And Cochlear Hair Cells, Gowri Nayak, Sue I. Lee, Rizwan Yousaf, Stephanie E. Edelmann, Claire Trincot, Christina M. Van Itallie, Ghanshyam P. Sinha, Maria Rafeeq, Sherri M. Jones, Inna A. Belyantseva, James M. Anderson, Andrew Forge, Gregory I. Frolenkov, Saima Riazuddin

Physiology Faculty Publications

The two compositionally distinct extracellular cochlear fluids, endolymph and perilymph, are separated by tight junctions that outline the scala media and reticular lamina. Mutations in TRIC (also known as MARVELD2), which encodes a tricellular tight junction protein known as tricellulin, lead to nonsyndromic hearing loss (DFNB49). We generated a knockin mouse that carries a mutation orthologous to the TRIC coding mutation linked to DFNB49 hearing loss in humans. Tricellulin was absent from the tricellular junctions in the inner ear epithelia of the mutant animals, which developed rapidly progressing hearing loss accompanied by loss of mechanosensory cochlear hair cells, while …

The Resilient Local Health Department: Surviving The 2008 Economic Crisis, Paul C. Erwin, Gulzar H. Shah, Glen P. Mays

Frontiers in Public Health Services and Systems Research

The purpose of this study was to identify potential modifiable factors that can protect local health departments (LHDs) from job losses and budget cuts during periods of economic stress. This was a retrospective cohort study based on the 2005 and 2010 surveys of LHDs conducted by the National Association of County and City Health Officials. The outcome of interest – resiliency of the LHD – represented financial resiliency for maintaining budgets during the 2008 recession, and was based on the ratio of observed-to-predicted expenditures per capita for 2010. LHDs which successfully weathered the economic recession of 2008 represented smaller populations …

A Survey Of The Barriers Associated With Academic-Based Cancer Research Commercialization, Nathan L. Vanderford, L. Todd Weiss, Heidi L. Weiss

Markey Cancer Center Faculty Publications

Commercialization within the academic setting is associated with many challenges and barriers. Previous studies investigating these challenges/barriers have, in general, broadly focused on multiple disciplines and, oftentimes, several institutions simultaneously. The goal of the study presented here was to analyze a range of barriers that may be broadly associated with commercializing academic-based cancer research. This goal was addressed via a study of the barriers associated with cancer research commercialization at the University of Kentucky (UK). To this end, a research instrument in the form of an electronic survey was developed. General demographic information was collected on study participants and two …