Digital Commons Network^™

Uncovering The Mechanism Of Reep2-Mediated Emt-Driven Membrane Trafficking In Lung Adenocarcinoma, Kevin Fulp

Theses and Dissertations--Toxicology and Cancer Biology

Membrane trafficking is frequently disrupted during cancer progression, and the underlying mechanisms remain largely unknown. Currently, no effective drugs target dysregulated membrane trafficking for cancer treatment. Recent evidence has demonstrated that epithelial-to-mesenchymal transition (EMT) in lung adenocarcinoma (LUAD) employs a membrane trafficking program to coordinate cancer cell invasion and immunosuppression. To further dissect the pro-tumorigenic membrane trafficking program, we initiated an in vivo CRISPRi screen to assess more than 2,000 membrane trafficking-related genes in a syngeneic mouse LUAD model. We identified REEP2, an endoplasmic reticulum (ER) shaping protein, as a novel regulator of EMT-driven membrane trafficking. High REEP2 expression is …

Reep2-Driven Pro-Metastatic Secretion Promotes Lung Cancer Progression, Oluwafunminiyi E. Obaleye

Theses and Dissertations--Toxicology and Cancer Biology

Membrane trafficking plays a critical role in cellular function and is frequently dysregulated in cancer to promote metastasis. In lung adenocarcinoma (LUAD), the epithelial-to-mesenchymal transition (EMT) activating transcription factor, ZEB1, drives a pro-metastatic membrane trafficking program; however, the underlying molecular mechanisms remain poorly understood. Using a CRISPR interference (CRISPRi) in vivo screen of 2,099 membrane trafficking regulators in a syngeneic mouse model of EMT-driven LUAD, we identified REEP2 — an endoplasmic reticulum (ER) shaping protein — as a critical regulator of tumor progression. REEP2 mRNA expression correlates with poor prognosis, EMT signatures, and an immunosuppressive tumor microenvironment in LUAD patients. …

Forage News [2026-02], University Of Kentucky

Forage News

No abstract provided.

Twenty-First Century Data Systems: Evolving Cancer Registries To A Learning Health System, Peter P. Yu, W. Scott Campbell, Eric B. Durbin, Lawrence N. Shulman, Jeremy L. Warner

Markey Cancer Center Faculty Publications

Introduction: Oncology is a data-rich environment reflecting the increasing incidence of cancer in the US aging population, transformation of cancer into a chronic disease due to advances in treatment, and the emergence of new data categories such as genomics. Concomitantly, healthcare systems are challenged to meet regulatory and voluntary reporting of cancer data to support government, quality certification, research and strategic partnership needs.

Materials and Methods: The National Academies of Science, Engineering, and Medicine organized a workshop entitled “Enabling 21st-century applications for cancer surveillance through enhanced registries and beyond” with representation from NCI Comprehensive Cancer Centers, oncology and medical informatics …

Implementing A “Naloxone Hub With Many Spokes” Model In Kentucky: Partner Agency Perspectives On Implementation Strategies, Hannah K. Knudsen, Michael Goetz, Shaquita Andrews-Higgins, Sandra Back-Haddix, Olivia A. Davis, Patricia R. Freeman, Douglas R. Oyler, Sharon L. Walsh

Behavioral Science Faculty Publications

Background: Overdose education and naloxone distribution (OEND) is a vitally important evidence-based practice for addressing the ongoing opioid epidemic. During the HEALing Communities Study in Kentucky, OEND was dramatically scaled up in eight counties using a “hub with many spokes” model and multifaceted implementation strategies. This aim of this manuscript is to describe qualitative perspectives of partner organizations regarding the utility of the implementation strategies used to expand OEND.   Method: Twenty small-group and 24 individual qualitative interviews were conducted with staff from 44 agencies that implemented OEND through partnerships with the study team. Interviews were conducted 6–8 months after the …

Clonal Expansion Of Cytotoxic Cd8⁺ T Cells In Lecanemab-Associated Aria, Lance A. Johnson, Kai Saito, Akhil V. Pallerla, Jessica L. Funnell, Ashley R. Ezzo, Chelsea M. Song, Douglas A. Harrison, Noah J. Norton, Lauren C. Moore, Linda J. Van Eldik, David W. Fardo, Greg E. Cooper, Josh M. Morganti

Sanders-Brown Center on Aging Faculty Publications

Amyloid-related imaging abnormalities (ARIA) are the principal safety concern limiting anti-amyloid therapies for Alzheimer’s disease, yet their biology remains unclear. Here we show, through multi-omic profiling of peripheral blood from three ARIA+ patients and matched controls, that ARIA is associated with coordinated reprogramming of CD8 + T cells. CD8+ effector memory (TEM) and terminally differentiated (TEMRA) subsets were expanded, clonally enriched, and transcriptionally primed for cytotoxicity and vascular trafficking. Transcription factor inference and metabolomics converged on glycolytic reprogramming favoring short-lived effector function. Ligand-receptor modeling revealed enhanced monocyte-to-T cell signaling through antigen presentation, adhesion, and chemokine axes, while integration with a …

Developing A Community-Driven, Locally Sourced Medically Tailored Meal Model: A Pilot Linking Healthcare, Farmers, And Patients, Julie Brown, Ashton Potter, Leandra Forman, Jairus J. Rossi, Anna Cason, Christa Mayfield, Alison Gustafson

Dietetics and Human Nutrition Faculty Publications

Background: Food is Medicine (FIM) programs improve health outcomes by integrating nutrition interventions into healthcare delivery, yet gaps remain in community partner capacity and local food system integration. This program aimed to build community capacity to develop a locally sourced, medically tailored meal (MTM) model connecting farmers, healthcare providers, and patients. Methods: Meals were developed by a registered dietitian following American Heart Association (AHA) Heart-Check and MyPlate nutrition standards. Recipes were adapted for freezing, scaled for production, and sourced from 26 central Kentucky farms. Eligible participants with hypertension or type 2 diabetes (T2D) were referred through the Food as Health …

Forage News [2026-03], University Of Kentucky

Forage News

No abstract provided.

From Anxiety To Stewardship: An Autoethnographic Study Of Therapist Development, Christie Ellis

Theses and Dissertations--Family Sciences

Routine outcome monitoring (ROM) is recognized as an evidence-informed practice for supporting psychotherapy outcomes, yet limited research has examined how therapists-in-training experience integrating ROM into their clinical development. This autoethnographic study investigated the role of ROM in shaping therapist identity development within a university-based marriage and family therapy training clinic. Qualitative data included 34 reflective journal entries analyzed using reflexive thematic analysis, and quantitative data included multiple client outcome trajectories across several clinical measures compared descriptively with prototypical change trajectories derived from MFT-PRN datasets. Qualitative findings reflected a developmental progression from initial anxiety and performance concerns toward increased confidence, attunement, …

Goal-Driven Shared Control In Eeg-Based Brain Machine Interface For Freewill Reaching And Grasping With Movement Intention Detection And Goal Position Decoding, Bhoj Raj Thapa

Theses and Dissertations--Electrical and Computer Engineering

Upper limb motor impairments can severely limit a person’s ability to perform everyday reaching and grasping tasks. Electroencephalogram (EEG)-based brain machine interfaces (BMIs) offer a non-invasive approach for translating neural activity into control signals for assistive devices such as robotic arms. However, traditional EEG-based BMI studies have generally focused on externally cued paradigms, where both movement timing and target selection are specified by the experimenter rather than freely chosen by the user. In addition, shared control offers a practical framework for assistive BMI operation by dividing responsibility between the user and the intelligent robotic system. However, in many EEG-based shared …

Bio-Based Oxalic Acid Production In Issatchenkia Orientalis Enables Sustainable Rare Earth Recovery, Jingxia Lu, Wenjun Guo, Ziye Dong, Sarang S. Bhagwat, Shih-I Tan, Zhixin Zhu, Andrew Johnson, Jeremy S. Guest, Rick Q. Honaker, Dan M. Park, Yongqin Jiao, Huimin Zhao

Mining Engineering Faculty Publications

The growing demand for rare earth elements (REEs) in clean energy and high-tech industries underscores the need for sustainable recovery methods and a reliable supply of processing chemicals. Here, we establish a microbial platform using the acid-tolerant yeast Issatchenkia orientalis SD108 to produce bio-oxalic acid for REE recovery. By introducing an oxaloacetate cleavage pathway and applying metabolic engineering, the engineered strain produces 39.53 g·L^-1 oxalic acid at pH 4.0 in fed-batch fermentation. The crude fermentation broth, used without purification, efficiently precipitates over 99% neodymium (Nd), 99% dysprosium (Dy), and 98% lanthanum (La) from individual REE chloride solutions. Recovery from …

Leadership And Literacy Assessment Systems: A Multi-Case Study Of System Coherence And Assessment Use In Mississippi Elementary Schools, Kristin P. Walters

Theses and Dissertations--Educational Leadership Studies

This qualitative embedded multiple-case study examined how school-level literacy leadership shaped the coherence and use of literacy assessment systems in five Mississippi Elementary Schools. Situated within the context of Mississippi’s recent literacy gains and the policy environment emerging from the Literacy-Based Promotion Act, this study explored how literacy leaders guide assessment systems to support coherence between literacy instruction and assessment practice.

Data were collected through semi-structured interviews with 25 educators across five elementary schools in different districts. Analysis was conducted using descriptive and values coding organized into an analysis matrix that facilitated within-case and cross-case exploration of patterns in distributed …

Making Inquiry Accessible: Inquiry-Based Design And Instruction For English Learners In Secondary Social Studies Classrooms, Meghan T. Hawkins

Theses and Dissertations--Education Sciences

This dissertation explores inquiry-based instruction for English Learners (ELs) in secondary social studies classes. The first article explored the existing literature in the field. The second article considered instructional design choices that could increase learning outcomes in an inquiry designed for ELs, implemented in a culturally and linguistically responsive classroom. Finally, the third article is an exploratory case study that examined how a secondary high school social studies teacher and ESL co-teacher designed and implemented inquiry-based instruction in a co-taught ESL class. The research questions included: 1. How do teachers build trust in a culturally and linguistically responsive social studies …

Culturally Sustaining Pedagogy Through Popular Music And Media In Elementary Music Education, Martina Vasil

Music Faculty Publications

Elementary music teachers in the United States face many challenges today, including an increasing cultural divide between teachers and students, worsening student behavior, and excessive exposure to technology in children’s lives. These challenges are magnified due to the hundreds of students elementary music teachers see weekly, the lack of teaching and planning time, and inadequate teaching resources, making it difficult to fully understand the culture and learning needs of every child. However, music educators may find culturally sustaining pedagogy (CSP) a useful tool for meeting the needs of a diverse student body. Further, when teachers engage in kid culture, the …

Dextromethorphan And Trace Level Detections Of Dextrorphan In Post-Race Urine Samples – A Case Report And Analysis, Kimberly Brewer, Justin C. Holmes, Clara Fenger, Abelardo Morales-Briceño, Andreas F. Lehner, George A. Maylin, Robert Holland, Levent Dirikolu, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Dextrorphan is a Phase I equine metabolite of dextromethorphan, a widely used anti-tussive / cough suppressant medication marketed and available in numerous over-the-counter formulations in the United States. Dextromethorphan is an Association of Racing Commissioners International (ARCI) drug class 4 penalty class B substance and a Horseracing Integrity and Welfare Authority (HIWU) CONTROLLED S7 B substance and is regulated as such in horse racing. In horses, dextromethorphan is metabolized to its Phase I metabolite, dextrorphan, which is next glucuronidated and the resultant dextrorphan-glucuronide conjugate then excreted at remarkably high concentrations in equine urine. Dextrorphan is therefore at times identified in …

The Association Between Human Papillomavirus Type 16 E6 Seroprevalence And Degrees Of Anal Disease Among Men With Hiv, Krystle A.Lang Kuhs, Christopher Otieno, Staci Sudenga, Tim Waterboer, Weiqun Tong, Dorothy Wiley, Jay H. Bream, Steven Wolinsky, Jeremy Martinson, Gypsyamber D’Souza

Epidemiology and Environmental Health Faculty Publications

Introduction: Human papillomavirus type 16 (HPV16) E6 antibodies may be an early biomarker of anal cancer. We cross-sectionally evaluated when in the course of anal disease, HPV16 antibodies are induced. Methods: A nested case-control study of 846 men who have sex with men (MSM) was conducted within a prospective study of men with and without HIV. Cases of anal HPV16 (N=262), biopsy-confirmed high-grade squamous intraepithelial lesion (HSIL;N=140), and anal cancer (N=21) were individually matched to controls (N=423) on HIV status, study-participation duration, and age. Serum samples closest to diagnosis underwent HPV serologic testing; prediagnostic serial samples were tested for anal …

Optimizing Control Definitions In Opioid Use Disorder Genetic Research Using Electronic Health Records, Maria Niarchou, Ellen L Tsai, Mariela V Jennings, Annika Faucon, Hyunjoon Lee, Kritika Singh, Justin D Tubbs, Panos Roussos, Tian Ge, Richard C Crist, Rachel Vickers-Smith, Howard Edenberg, Amy Moore, Bradley T Webb, Eric O Johnson, Rachel L Kember, Jordan W Smoller, Lea K Davis, Brandon J Coombes, Georgios Voloudakis, David Burstein, Travis T Mallard, Vanessa Troiani, Sandra Sanchez-Roige

Epidemiology and Environmental Health Faculty Publications

Amidst the opioid crisis, understanding the genetic basis of opioid use disorder (OUD) is crucial for identifying biological mechanisms and intervention points. However, genome-wide association studies (GWASs) have been hampered by inadequate sample sizes and often the use of control populations not assessed for prior opioid exposure. Because opioid exposure is a prerequisite for the development of OUD, consideration of exposure history in controls is important. Electronic health record data (EHR) paired with genomic information allow a broader sampling of patients with OUD and exposed controls. We leveraged data across two healthcare systems to evaluate the impact of using controls …

Shaping The Future Of Human Biomonitoring (Hbm), Maryam Zare Jeddi, Nancy B. Hopf, Karen S. Galea, Kate Jones, Henriqueta Louro, Maria João Silva, Adrian Covaci, Tiina Santonen, Paul T.J. Scheepers, Susana Viegas, Lesliam Quirós-Alcalá, Asif Qureshi, M. Elizabeth Marder, Natalie Von Goetz, Konstantinos M. Kasiotis, Kyriaki Machera, Ovnair Sepai, Radu Corneliu Duca, Manosij Ghosh, An Van Nieuwenhuyse, Ming Kei Chung, Jihyon Kil, Shoji F. Nakayama, Aziza Menouni, Kaoutar Chbihi, Ana Maria Vekic, Gustavo Souza, Maisarah Nasution Waras

Epidemiology and Environmental Health Faculty Publications

Human biomonitoring (HBM) continues to play an indispensable role within exposure science, offering insights into aggregate chemical exposures across populations and life stages. Since 2018, the European chapter of the International Society of Exposure Science Human Biomonitoring Working Group (ISES Europe HBM WG) has aimed to facilitate generation of more and high-quality HBM data. The working group aims to strengthen integration of HBM data into regulatory frameworks through improved study design, harmonized methodologies, and enhanced reporting practices. Key achievements in the past seven years include the harmonization of HBM metadata through development of minimum information requirements for HBM (MIR-HBM), development …

Building The Ryan White Hiv/Aids Program Bench In Kentucky, Lauren Bifulco, Kathleen Harding, Jana Collins, Anna Rogers, Lenon Farkash, Marwan Haddad, Alice Thornton

Epidemiology and Environmental Health Faculty Publications

The Ryan White HIV/AIDS program (RWHAP) is governed by legislative and programmatic guidelines that ensure comprehensive services are provided to persons with HIV (PWH) across the United States. Comprehensive training and skill building opportunities for programmatic staff are rare. RWHAP funding recipient organizations may struggle to acquire and retain essential program-specific knowledge, with downstream impact on patient care and treatment. We designed a comprehensive 11-session videoconference learning series to improve program staff’s confidence and competence with RWHAP fundamentals. Quantitative pre-vs-post session data were analyzed using Wilcoxon Signed-Rank tests, and qualitative data using thematic analysis. We enrolled 136 staff from 23 …

Decreased Gabapentin Prescription Fills Among Medicaid Enrollees Following State-Level Schedule V Controlled Substance Classification In Kentucky And West Virginia, Katherine Gora Combs, Stephen W. Marshall, Rachel Vickers-Smith, Alan Kinlaw, Nabarun Dasgupta, Juan M. Hincapie-Castillo

Epidemiology and Environmental Health Faculty Publications

Background: Driven by concerns of gabapentin misuse and other harms, Kentucky (KY) and West Virginia (WV) became the first states to classify gabapentin as a schedule V controlled substance in July 2017 and June 2018, respectively. This study examined the association of these policies with gabapentin utilization among Medicaid beneficiaries. Methods: Using a controlled interrupted time series design, we modeled a segmented generalized least squares regression and calculated the immediate and trend changes of gabapentin prescriptions among Medicaid enrollees in 2 states (KY, WV), separately. North Carolina (NC) was used as a control to account for secular changes; there was …

Structured Laser Vision-Based Measurement Of Gta-Weld Pool, Gang Zhang, Jianbo Wang, Yu Shi, Ding Fan, Yuming Zhang

Electrical and Computer Engineering Faculty Publications

The current study of weld pool fluid dynamics in arc welding focuses on the numerical model establishment and simulation, and the x-ray combined with particle trace imaging observations, there is no real-time monitor and quantitatively characterize the weld pool flow behavior in welding process for controlling the weld quality. This study develops an innovative structured laser vision-based sensing system for three-dimensional (3D) reconstruction and quantitative analysis of weld pool surface topographies in gas tungsten arc welding (GTAW). Through characterization of dynamic weld pool morphologies, two novel parameters are proposed: the surface convexity variation rate (R_h) and fluid …

“Policymakers Need To See This!”, Ramona G. Olvera, Peter Balvanz, Owusua Yamoah, Kesla Klingler, Marisa D. Booty, Sylvia A. Ellison, Amy Farmer, Amy K. Kuntz, Hilary L. Surratt, Carrie B. Oser, Margaret L. Mcgladrey

Sociology Faculty Publications

The use of Photovoice methods to identify and address public health challenges has evolved since this participatory action research method’s inception in the 1990s. However, there is a notable lack of research on the dissemination of Photovoice findings to promote community action and change. This study evaluates audience and Photovoice participants’ experiences of dissemination events presenting the results of 21 Photovoice projects that supplemented the HEALing Communities Study (HCS), a multistate community-based study of strategies to reduce opioid overdose deaths. The Photovoice projects captured the perspectives of people with lived experience of substance use disorder and front-line providers regarding their …

Understanding Resilience In Children In Out-Of-Home Care: Using Person-Centered Health Services Research, Hyeeun K. Shin

Theses and Dissertations--Health Services Research (HSR)

Adverse childhood experiences (ACEs), which refer to exposure to traumatic life events before age 18, and instability in caregiving experiences contribute to poor physical, mental, behavioral, and developmental health outcomes for both children and adults. Previous research shows that these experiences can have negative effects both in the short term and throughout a person’s life. However, children’s strengths, which are the internal and external assets that support their development, can help protect against the impact of ACEs. Effective parenting, positive social support, and self-regulation skills are essential factors that build resilience, helping individuals adapt to challenges that may affect their …

The Digital In The Library: Finding The Mutually Beneficial Synergy, James M. Donovan

Law Faculty Books and Chapters

With the value of access to digital resources abundantly demonstrated during the COVID-19 lockdown, enthusiasm for the format is reaching new heights. This chapter counsels restraint, rebutting the argument that skepticism concerning the wide adoption of digital materials into the library is due merely to nostalgia by anti-technologists for a now-bygone age. Despite their obvious usefulness in certain situations, patrons have not responded well when libraries propose to discard legacy print collections in favor of transient electronic formats.To explain this rejection, this chapter argues that libraries are built upon principles of collection development and organization of materials in order to …

College Student Perceptions Of A Pilot Cooking And Nutrition Education Workshop Through The Lens Of The Social Cognitive Theory: A Qualitative Study, Emma Watras, Amelia Sullivan, Emalee Harrington, Mona Therrien-Genes, Kathryn Yerxa, Makenzie Barr-Porter, Jade Mcnamara

Dietetics and Human Nutrition Faculty Publications

This study qualitatively examined college students' perceptions of a pilot cooking and nutrition workshop, College Cooking Connection (CCC), through the Social Cognitive Theory (SCT) framework. Undergraduate students who attended at least one CCC workshop (N = 29) were invited to a 30 min virtual focus group. A trained moderator guided the discussion to gather perceptions of the program. Themes were identified using thematic analysis with a directive and deductive approach based on SCT. Focus groups revealed eight major themes across the SCT. Cognitive themes included: (1) improved nutrition literacy, (2) understanding the nutrition-mental health connection, and (3) increased awareness of …

Expressing Experiences Of Being Gender Non-Conforming In The “Ladies” Restroom Through Autoethnography-Based Research Poetics, Ellen D.B. Riggle

Gender and Women's Studies Faculty Publications

Three research poems are created as an autoethnographic response to feedback on a research article on the author’s experiences as a gender non-conforming person in women’s public restrooms. The feedback loop includes the sharing of lived experiences by others and theoretical (why?) discussions in the context of the gendered policing and politicalization of women’s restrooms. The first found poem includes words, phrases, and descriptions of images used to label spaces designated as women’s restrooms. The second prose poem recounts the author's experiences through a reframing of risk to women’s health, understanding the cultural context of male entitlement and White supremacy, …

Legal Professional Identity Development: An Exploration Of The Social And Emotional Processes By Which Law Students Transition From Novices To Professionals, David Hall Abney Ii

Theses and Dissertations--Communication

This dissertation seeks to heed the call for expansion of instructional communication research (ICR) beyond the undergraduate classroom and undergraduate learning objectives by exploring older learners and higher-order affective learning objectives. Legal professional identity development (LPID) is a discipline-specific type of higher-order affective learning that is conceptualized as a continuous nonlinear process that begins in law school and extends throughout a lawyer’s career, involving the internalization of the legal profession’s core values and norms and their integration with an evolving identity. Using a pragmatic ICR approach and a mixed-methods design, Phase 1 employed individual interviews and focus groups to investigate …

“We Do Things A Little Different Exactly As You'd Expect Here...": Egocentric Network Structures As Predictors Of Organizational Culture Type, Ray Celeste S. Tanner

Theses and Dissertations--Communication

This dissertation examines organizational culture as an outcome of communication structures by integrating the Competing Values Framework (CVF) with egocentric network analysis. Although CVF is one of the most widely used frameworks for diagnosing organizational culture and predicting organizational effectiveness, prior research has overwhelmingly treated culture as an independent variable. Responding to calls for greater attention to the antecedents of culture, this study reconceptualizes organizational culture as a dependent variable and investigates how individuals’ perceived communication networks predict perceptions of CVF culture types. Using egocentric network data collected from employees across a diverse range of organizations and industries, this study …

Can Love Bloom On A Battlefield: An Analysis Of Queer Militaristic Fanfiction, Brandon Bost

Theses and Dissertations--Communication

It is noted that readers and fans hold unique and valid interpretations of texts, interpretations that become expressed in fan works like fan art, memes, or the subject of this study, fanfiction. This study performs textual analysis on queer fanfiction in Metal Gear Solid and Code Geass fandoms. It examines the unique relationship militarism holds with intimacy, and how intimacy intersects with violence and control in unique ways in these fan texts. Within these fanfics, militarism can serve as a barrier to intimacy, forcing relationships to adapt to militaristic structures within these fan interpretations.

Exploring The Relationship Between Self-Esteem And Communication Among Equestrians In Facebook Groups, Jenna Young

Theses and Dissertations--Communication

People’s interpersonal relationships and social groups are connected to their self-esteem. Equestrians, a social group unified around riding or working with horses, form identities based on their involvement in the equine industry, whether that be recreationally or professionally. Equestrians’ subjective views of self-esteem may be related to their overall well-being. Theories related to self-esteem and identity indicate that communication within social relationships, like within the equine industry, may impact an individuals’ sense of self-worth. Facebook groups have increased in popularity in the equine industry due to the ease of communication between equestrians. Therefore, this study investigated the relationship between self-esteem …