Digital Commons Network^™

Lexington Humane Society: Analysis Of Volunteers, D'Arcy Robb

MPA/MPP/MPFM Capstone Projects

The Lexington Humane Society depends substantially on volunteer labor yet suffers from a high volunteer attrition rate. Using data from the organization’s database and a survey of volunteers, this project paints a demographic picture of the volunteer population and identifies traits that affect volunteer longevity.

The evidence shows that the organization’s volunteers are overwhelmingly likely to be pet-owning women with no children at home who are motivated to volunteer because it allows them to act on their values. Middle-age individuals are more likely than those under 25 or over 65 to become long term volunteers, and individuals who have recently …

Public Art Master Planning For Municipal Governments: Core Components And Common Practices, Jonathan Hollinger

MPA/MPP/MPFM Capstone Projects

Public art master planning for municipal government is the process of strategically planning the placement of public art objects, and the processes used to administer a public art program. It is a broad process that combines urban design and planning, artistic practice, public policy, local government management, and many other elements.

Due to this broad background, the master plan of a public art program is a complex document with many requirements. This analysis attempts to answer the following research questions:

• What is the common process for developing a public art master plan?
• What are the core components of a public …

Understanding Non-Profit Organizations In States With Appalachian Counties: Where They Locate And The Missions They Serve, Rebecca E. Tucker

MPA/MPP/MPFM Capstone Projects

No executive summary.

Elucidating The Biochemical Wizardry Of Triterpene Metabolism In Botroycoccus Braunii, Thomas Daniel Niehaus

Theses and Dissertations--Plant and Soil Sciences

B. braunii is a green alga that has attracted attention as a potential renewable fuel source due to its high oil content and the archeological record of its unique contribution to oil and coal shales. Three extant chemotypes of B. braunii have been described, namely race A, race B, and race L, which accumulate alkadienes and alkatrienes, botryococcene and squalene and their methylated derivatives, and lycopadiene, respectively. The methylated triterpenes, particularly botryococcenes, produced by race B can be efficiently converted to high quality combustible fuels and other petrochemicals; however, botryococcene biosynthesis has remained enigmatic.

It has been suggested that botryococcene …

The Federalization Of The Duty Of Loyalty Governing Charity Fiduciaries Under United States Tax Law, Johnny Rex Buckles

Kentucky Law Journal

No abstract provided.

Choking Out Local Community Service Organizations: Rising Federal Tax Regulation And Its Impact On Small Nonprofit Entities, Nicole S. Dandridge

Kentucky Law Journal

No abstract provided.

The "Federalization" Problem And Nonprofit Self- Regulation: Some Initial Thoughts, Mark Sidel

Kentucky Law Journal

No abstract provided.

Drawing The Line At Pushing "Play": Barring Video Montages As Victim Impact Evidence At Capital Sentencing Trials, Alicia N. Harden

Kentucky Law Journal

No abstract provided.

Rit Gtpase Signaling Mediates Oxidative Stress Resistance And Survival Of Adult Newborn Neurons After Traumatic Brain Injury, Weikang Cai

Theses and Dissertations--Molecular and Cellular Biochemistry

The small GTPases function as molecular switches to control diverse signaling cascades. The mammalian Rit and Rin, along with Drosophila Ric, comprise an evolutionarily conserved subfamily of the Ras-related GTPases. Previous studies using cultured cell models suggested that Rit was involved in the control of cell proliferation, transformation, neuronal differentiation, morphogenesis, and cell survival, but the principal physiological function of Rit remained uncharacterized.

To address this outstanding question, we employed a genetic approach, engineering a Rit knockout mouse. Using this animal model, we demonstrate a central role of Rit in governing cell survival in a p38-dependent fashion. Primary mouse embryonic …

Trichloroethylene Exposure And Traumatic Brain Injury Interact And Produce Dual Injury Based Pathology And Pioglitazone Can Attenuate Deficits Following Traumatic Brain Injury, Andrew David Sauerbeck

University of Kentucky Doctoral Dissertations

The development of Parkinson's disease (PD) in humans has been linked to genetic and environmental factors for many years. However, finding common single insults which can produce pathology in humans has proved difficult. Exposure to trichloroethylene (TCE) or traumatic brain injury (TBI) has been shown to be linked to PD and it has also been proposed that multiple insults may be needed for disease development.

The present studies show that exposure to TCE prior to a TBI can result in pathology similar to early PD and that the interaction of both insults is required for impairment in behavioral function, and …

Assessing The Perceptions Of Environmental Pollutants, Health And Nutrition Behavior To Improve Risk Communications In Kentucky, Elizabeth Virginia Willett

University of Kentucky Master's Theses

Nutrition interventions are an effective way to improve the dietary habits and lifestyle choices and reduce the risk of chronic disease. The Researchers in the UK-SRP Community Engagement Core develop nutrition programs for communities affected by environmental pollutants. Risk communication is a discipline that can be used to develop targeted nutrition interventions that will yield positive behavior change. The purpose of this study was to examine knowledge, risk perception, and actions concerning environmental pollutants and nutrition behavior. Data was collected using a modified survey instrument based on the validated Environmental Health Engagement Profile (EHEP). Survey participants from diverse regions of …

Gaze Estimation Using Sclera And Iris Extraction, Prashanth Rao Periketi

University of Kentucky Master's Theses

Tracking gaze of an individual provides important information in understanding the behavior of that person. Gaze tracking has been widely used in a variety of applications from tracking consumers gaze fixation on advertisements, controlling human-computer devices, to understanding behaviors of patients with various types of visual and/or neurological disorders such as autism. Gaze pattern can be identified using different methods but most of them require the use of specialized equipments which can be prohibitively expensive for some applications. In this dissertation, we investigate the possibility of using sclera and iris regions captured in a webcam sequence to estimate gaze pattern. …

Participation In Agricultural Governmental Cost Share Programs In The Kentucky River Watershed, Pedro Miguel Fernandes Da Costa

University of Kentucky Master's Theses

The purpose of this study is to review existing literature of factors that influence farmers’ decision to participate in conservation programs. This study is also intended to collect county data and information on conservation programs and participation in the Kentucky River watershed region, which can be analyzed and used to draw differences in characteristics of the region that would suggest willingness to participate in a trading scheme for improvements in water quality.

The results suggest that more participation in a trading scheme from some counties than others should be expected. Counties with more farms and larger farms will probably have …

Practice And Application Of Knowledge By Nutrition Students, Jennifer Kristin Boyee

University of Kentucky Master's Theses

Practice and knowledge of upper‐level human nutrition and dietetics students (juniors and seniors, n=96) attending a midwestern university were compared to their comprehension and application of the Scope of Dietetics Practice. The sample consisted of 96 students, primarily (72%) in the 21 – 23 age category; females made up 83% and males comprised the remaining 17% of the sample. In this sample, 70% of the participants reported dietetics as their current major, and 30% stated that their major was human nutrition. Overall, students majoring in human nutrition responded neutrally to the questions concerning comprehension, knowledge, and implementation of the Scope …

Functional Characterization Of Wd Repeat Proteins, Atcstf50 And Atfy In Cleavage And Polyadenylation, Lavanya Dampanaboina

Theses and Dissertations--Plant and Soil Sciences

Polyadenylation is an essential post-transcriptional modification resulting in a mature mRNA in eukaryotes. Three cis-elements the Far Upstream Element (FUE), Near Upstream Element (NUE), and Cleavage Site (CS) - guide the process of cleavage and polyadenylation with the help of multi-subunit protein complexes cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF) along with cleavage factors and poly(A) polymerase. Protein-protein interactions play an important role in the cleavage and polyadenylation process. WD repeat proteins play an important role in protein-protein interactions and have diverse functions in plant system. In the present study WD repeat proteins AtCstF50 and AtFY were …

Methionine-35 Of Aβ(1-42): Importance For Oxidative Stress In Alzheimer Disease, D. Allan Butterfield, Rukhsana Sultana

Chemistry Faculty Publications

Alzheimer disease (AD) is an age-related progressive neurodegenerative disorder. This devastating disease is characterized by the presence of senile plaques (SP), neurofibrillary tangles (NFTs), and loss of synapses. Amyloid beta-peptide 1–42 (Aβ(1–42)) is the main component of SP and is pivotal to AD pathogenesis. Brain of subjects with AD and arguably its earliest manifestation, mild cognitive impairment (MCI), demonstrate increased levels of oxidative stress markers. Our laboratory combined these two aspects of AD and MCI and proposed the Aβ(1–42)-associated free radical oxidative stress hypothesis to explain oxidative stress under which the MCI and AD brain exist …

Synthesis And Characterization Of Polymeric Antioxidant Delivery Systems, Paritosh P. Wattamwar

Theses and Dissertations--Chemical and Materials Engineering

Even though the role of oxidative stress in a variety of disease states is known, strategies to alleviate this oxidative stress by antioxidants have not been able to achieve clinical success. Particularly, treatment of oxidative stress by small molecule antioxidants has not received due attention because of the challenges associated with its delivery. Antioxidant polymers, where small molecule antioxidants are incorporated into the polymer backbone, are an emerging class of materials that can address some of these challenges.

In this work, biodegradable polymers incorporating phenolic antioxidants in the polymer backbone were synthesized. Antioxidant polymers were then characterized for their in …

Opioid Codrugs For Pain Management, Ujjwal Chakraborty

Theses and Dissertations--Chemistry

Pain is an unpleasant sensory and emotional experience associated with actual or potential tissus damage or described in terms of such damage. Opioids are effective in treating moderate to severe pain, but opioid alone therapy is associated with several adverse effects, development of tolerance and addiction potential. One way to solve these problems is to administer opioids with adjuvant drugs. In this project several opioid molecules were combined with other adjuvant drugs in a single chemical entity to form a codrug.

A series of codrugs were prepared by conjugation of an opioid with S-(-)-nornicotine, ketamine, norketamine and gabapentin. Several …

Effects Of A Short-Term Mindfulness Intervention On Depression And Immune Function, Erin C. Walsh

Theses and Dissertations--Psychology

Pro-inflammatory cytokines have been implicated in the pathophysiology and maintenance of depression. This study investigated the effects of a short mindfulness intervention on pro-inflammatory correlates of depression (IL-6 and TNF-α) and selfreported psychological health. Sixty-four college females were assigned to a four-week mindfulness training group or a contact-control group. Cytokines and psychological health were assessed at baseline, post-treatment, and 3-month follow-up (mindfulness group only). IL-6 and TNF-α significantly decreased from baseline to post-treatment in the mindfulness group only; these changes were sustained at 3-month follow-up. No between-group differences in psychological health emerged. Although reductions in proinflammatory cytokines in the mindfulness …

Role Of Angiotensin Converting Enzyme 2 (Ace2) In Obesity-Associated Hypertension, Manisha Gupte

University of Kentucky Doctoral Dissertations

The purpose of this research was to determine whether adipocytes express ACE2 and its role in obesity-associated hypertension with diet-induced obesity.

To determine if ACE2 was expressed in adipose tissue and its regulation in the setting of diet-induced obesity, we fed male mice either a low fat (LF) or high fat (HF) diet acutely (1 week) or chronically ( 4 months). We demonstrated that ACE2 was regulated specifically in adipose tissue with consumption of a HF diet. However, with chronic HF feeding adipose ACE2 was dysregulated resulting in activation of the systemic RAS and increased blood pressure.

To determine the …

Disjuncture Among Classic Period Cultural Landscapes In The Tuxtla Mountains, Southern Veracruz, Mexico, Wesley Durrell Stoner

University of Kentucky Doctoral Dissertations

Teotihuacan was the most influential city in the Classic Mesoamerican worldsystem. Like other influential cities in the ancient world, however, Teotihuacan did not homogenously affect the various cultural landscapes that thrived in Mesoamerica during the Classic period (300-900 CE). Even where strong central Mexican influences appear outside the Basin of Mexico, the nature, extent, and strength of these influences are discontinuous over time and space. Every place within the Classic Mesoamerican landscape has a unique Teotihuacan story. In the Tuxtla Mountains of southern Veracruz, Mexico, Matacapan, located in the Catemaco Valley, drew heavily upon ideas and symbols fostered at Teotihuacan, …

Character Education And 4-H Youth Development, Jessica Lynn Mullins

University of Kentucky Master's Theses

Character education assumes responsibility for influencing and educating youth on morals, values, beliefs and positive character attributes in order to influence them into being positive and contributing members of society. Character education is most often taught in the home, schools and community. One community organization that integrates character education into its programming is 4-H Youth Development. The purpose of this research is to view the relationships between character education and 4-H, and more so whether Kentucky 4-H programs influence the knowledge, attitudes and practices of positive character among youth leaders. This study also looked at length of 4-H involvement and …

Beef Cattle Grazing Preference Of Tall Fescue As Affected By Endophyte, Herbert Troye Owens Iii

University of Kentucky Master's Theses

Many factors control, contribute to, stimulate and limit forage selection. It is apparent that cattle prefer certain cultivars compared to others. This study sought to test if cattle displayed preferences for certain cultivars over a two-year period in 2008 and 2009. Determining the effect of endophyte status on preference was another objective. Previous research showed rapid increase in the selection of preferred cultivars, i.e., diet learning. We attempted to replicate those results. Forty cultivars (34 tall fescue (Lolium arundinaceum (Schreb.) Darbysh.) cultivars), two festuloliums (Festuca pratensis x Lolium perenne), two meadow fescue (Lolium pratense) and one meadow …

Becoming Bodies: How Preadolescent Girls Consume And Produce Media In 21St Century America, Margaret Louise Mcgladrey

University of Kentucky Master's Theses

This study investigates preadolescent girls’ interpretations of images of and messages about women’s bodies presented in both traditional and online media in the American cultural context. Using qualitative methods including in-depth interviews, email diaries, and digital photo collages, this study gives voice to girls aged nine to eleven from diverse racial and socioeconomic backgrounds so that they might tell their stories about interacting with media that is relevant to their relationships with their bodies. Employing objectification theory as well as concepts from the cultural studies tradition, the findings suggest that the process of becoming a female body in the 21 …

Engineering Proteins With Unique Characteristics For Diagnostics And Biosensors, Smita Joel

University of Kentucky Doctoral Dissertations

Proteins possess a broad range of structural and functional properties and, therefore, can be employed in a variety of biomedical applications. While a good number of protein-based biosensing systems and biosensors for target analytes have been developed, the search for versatile, highly sensitive and selective sensors with long term stability able to provide fast detection of target analytes continues to be a challenge. To that end, we now report the design and development of modified proteins with tailored characteristics and their further utilization in the development of biosensing systems.

We take advantage of binding proteins that undergo a change in …

Structures, Roles And Relationships Within Public Health’S Response To The 2009-2010 H1n1 Outbreak: The Ties That Bind Public Information Officers And Emergency Risk Communication Efforts, Kathleen G. Vidoloff

University of Kentucky Doctoral Dissertations

Little is known about the role of public health public information officers (PIOs) during public health emergencies. This study uses interpretative methods to learn about the organizational structures that facilitate and constrain emergency risk communication efforts during public health emergencies. Interpretive thematic comparative analysis of PIOs experiences and reflections about their involvement in the 2009-2010 H1N1 response will be used to illustrate how social interactions between and among PIOs, public health staff, and representatives from other agencies create implicit and explicit structures that facilitate and constrain emergency risk communication. The application of three specific concepts from structuration theory, namely, agent, …

Going On Otor: Disaster, Mobility, And The Political Ecology Of Vulnerability In Uguumur, Mongolia, Daniel J. Murphy

University of Kentucky Doctoral Dissertations

Post-socialist states have increasingly adopted rural governance and resource management policies framed around the concepts of decentralization, devolution, and de-concentration in which formerly central state powers are transferred to lower, more local levels of governance. In more recent incarnations, these policies have become inspired by neo-liberal discourses of minimal government, self-rule, and personal responsibility. Increasingly, the social science literature has argued that such forms of neo-liberal governance lead to a variety of unforeseen and diverse consequences. This dissertation attempts to understand the impact of these political transformations on household vulnerability in the context of hazardous events called zud. I …

Pollution Knowledge And Urban Water Politics In The Ganges River Basin (India), Sya Buryn Kedzior

University of Kentucky Doctoral Dissertations

Millions of people rely upon the Ganges River as a source of water provision and a site of disposal for sewage, solid waste, agricultural runoff and industrial effluent. The river is also a goddess in the Hindu pantheon who is worshipped for her purificatory powers, despite water quality levels that fall far short of standards for use in bathing, washing, and drinking. In recent years, a number of non-governmental organizations (NGOs) have formed to oppose both pollution of the river and the failure of state-run pollution abatement programs. They are joined by an increasingly frequent number of seemingly spontaneous protests …

Circadian And Homeostatic Regulation Of Sleep In Cast/Eij And C57bl/6j Mice, Peng Jiang

University of Kentucky Doctoral Dissertations

Sleep is essential for mammals and possibly for all animals. Advancing our knowledge of sleep regulation is crucial for the development of interventions in sleep-related health and social problems. With this aim, this study utilizes laboratory mice to explore sleep regulatory mechanisms at behavioral, molecular, and genetic levels.

Sleep is regulated by the interaction of circadian and homeostatic processes. The circadian clock facilitates sleep to occur at a favorable time of the day. Normal mice, such as the C57BL/6J (B6) strain, sleep mostly during the day and initiate activities at dark onset. Here, I show mice of the CAST/EiJ (CAST) …

Regulation Of Abcg5 And Abcg8 Sterol Transporters In Biliary Cholesterol Elimination, Reverse Cholesterol Transport And Dyslipidemia, Nadezhda Steliyanova Sabeva

University of Kentucky Doctoral Dissertations

ATP-binding cassette transporters ABCA1 and ABCG1 initiate reverse cholesterol transport generating HDL particles, whereas ABCG5/G8 promote biliary cholesterol secretion thereby facilitating the last step of reverse cholesterol transport. Mutations in the leptin axis result in obesity and dyslipidemia in ob/ob and db/db mice. These mice have defective HDL clearance, increased plasma cholesterol and decreased biliary cholesterol elimination. My studies demonstrate that ABCG5/G8 protein is low in these animals and can be restored with caloric restriction or leptin replacement. To directly test whether ABCG5/G8 alone is able to correct reverse cholesterol transport defect, liver specific ABCG5/G8 expression was achieved in db/db …