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New Tall Fescue Varieties For The Future, Timothy D. Phillips

Forage Symposium at the Kentucky Cattlemen’s Convention

Over the past few decades forage producers have seen several major changes in varieties of tall fescue. From the days of Kentucky 31 being the predominant variety, the first change was the first generation of endophyte-free tall fescue varieties. For many growers, these have been useful additions, especially when coupled with rotational grazing to reduce the risks of overgrazing, and practices of eradication of stands of toxic endophyte pastures and prevention of the reintroduction of toxic endophyte tall fescue seed into stands of endophyte-free varieties. However, overall these varieties often lacked the persistence of Kentucky 31 infected with the common …

Tall Fescue In Kentucky: The First 80 Years, Garry D. Lacefield

Forage Symposium at the Kentucky Cattlemen’s Convention

Tall fescue is a native of Europe. The exact date of its’ introduction into the United States is not known, but likely came as a contaminant in meadow fescue seed from England prior to 1880 (Figure 1). Tall Fescue was an entry in the National Herbarium Collection in 1879 and was grown in plots in Utah, Kentucky and Maryland (USDA) in 1880. In 1916, tall fescue plants were identified in Pullman, Washington with some of these plants taken to Corvallis, Oregon in 1918. Selections out of this initial planting were released in 1945 as the variety Alta.

Foreword [2012], Garry D. Lacefield, Christi L. Forsythe

Forage Symposium at the Kentucky Cattlemen’s Convention

No abstract provided.

A High Density Snp Array For The Domestic Horse And Extant Perissodactyla: Utility For Association Mapping, Genetic Diversity, And Phylogeny Studies, Molly E. Mccue, Danika L. Bannasch, Jessica L. Petersen, Jessica Gurr, Ernie Bailey, Matthew M. Binns, Ottmar Distl, Gérard Guérin, Telhisa Hasegawa, Emmeline W. Hill, Tosso Leeb, Gabriella Lindgren, M Cecilia T. Penedo, Knut H. Røed, Oliver A. Ryder, June E. Swinburne, Teruaki Tozaki, Stephanie J. Valberg, Mark Vaudin, Kerstin Lindblad-Toh, Claire M. Wade, James R. Mickelson

Veterinary Science Faculty Publications

An equine SNP genotyping array was developed and evaluated on a panel of samples representing 14 domestic horse breeds and 18 evolutionarily related species. More than 54,000 polymorphic SNPs provided an average inter-SNP spacing of ∼43 kb. The mean minor allele frequency across domestic horse breeds was 0.23, and the number of polymorphic SNPs within breeds ranged from 43,287 to 52,085. Genome-wide linkage disequilibrium (LD) in most breeds declined rapidly over the first 50-100 kb and reached background levels within 1-2 Mb. The extent of LD and the level of inbreeding were highest in the Thoroughbred and lowest in the …

Usp8 Promotes Smoothened Signaling By Preventing Its Ubiquitination And Changing Its Subcellular Localization, Ruohan Xia, Hongge Jia, Junkai Fan, Yajuan Liu, Jianhang Jia

Molecular and Cellular Biochemistry Faculty Publications

The seven transmembrane protein Smoothened (Smo) is a critical component of the Hedgehog (Hh) signaling pathway and is regulated by phosphorylation, dimerization, and cell-surface accumulation upon Hh stimulation. However, it is not clear how Hh regulates Smo accumulation on the cell surface or how Hh regulates the intracellular trafficking of Smo. In addition, little is known about whether ubiquitination is involved in Smo regulation. In this study, we demonstrate that Smo is multi-monoubiquitinated and that Smo ubiquitination is inhibited by Hh and by phosphorylation. Using an in vivo RNAi screen, we identified ubiquitin-specific protease 8 (USP8) as a deubiquitinase that …

Conformational Altered P53 As An Early Marker Of Oxidative Stress In Alzheimer's Disease, Laura Buizza, Giovanna Cenini, Cristina Lanni, Giulia Ferrari-Toninelli, Chiara Prandelli, Stefano Govoni, Erica Buoso, Marco Racchi, Maria Barcikowska, Maria Styczynska, Aleksandra Szybinska, D. Allan Butterfield, Maurizio Memo, Daniela Uberti

Sanders-Brown Center on Aging Faculty Publications

In order to study oxidative stress in peripheral cells of Alzheimer's disease (AD) patients, immortalized lymphocytes derived from two peculiar cohorts of patients, referring to early onset AD (EOSAD) and subjects harboured AD related mutation (ADmut), were used. Oxidative stress was evaluated measuring i) the typical oxidative markers, such as HNE Michel adducts, 3 Nitro-Tyrosine residues and protein carbonyl on protein extracts, ii) and the antioxidant capacity, following the enzymatic kinetic of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRD). We found that the signs of oxidative stress, measured as oxidative marker levels, were evident only in ADmut …

Hiv-1 Tat Triggers Nuclear Localization Of Zo-1 Via Rho Signaling And Camp Response Element-Binding Protein Activation, Yu Zhong, Bei Zhang, Sung Yong Eum, Michal Toborek

Neurosurgery Faculty Publications

The human immunodeficiency virus (HIV)-specific protein trans-activator of transcription (Tat) can contribute to the dysfunction of brain endothelial cells and HIV trafficking into the brain by disrupting tight junction (TJ) integrity at the blood–brain barrier (BBB) level. Specific TJ proteins, such as zonula occludens (ZO) proteins, localize not only at the cell–cell borders but are also present in the nuclei. The objective of the present study was to evaluate the mechanisms and significance of Tat-induced nuclear localization of ZO-1. Treatment of a brain endothelial cell line (hCMEC/D3 cells) with Tat resulted in a decrease in total levels of ZO-1 but …

The Complete Mitochondrial Genome And Novel Gene Arrangement Of The Unique-Headed Bug Stenopirates Sp. (Hemiptera: Enicocephalidae), Hu Li, Hui Liu, Aimin Shi, Pavel Stys, Xuguo Zhou, Wanzhi Cai

Entomology Faculty Publications

Many of true bugs are important insect pests to cultivated crops and some are important vectors of human diseases, but few cladistic analyses have addressed relationships among the seven infraorders of Heteroptera. The Enicocephalomorpha and Nepomorpha are consider the basal groups of Heteroptera, but the basal-most lineage remains unresolved. Here we report the mitochondrial genome of the unique-headed bug Stenopirates sp., the first mitochondrial genome sequenced from Enicocephalomorpha. The Stenopirates sp. mitochondrial genome is a typical circular DNA molecule of 15, 384 bp in length, and contains 37 genes and a large non-coding fragment. The gene order differs substantially from …

Low Income Preschoolers' Non-Parental Care Experiences And Household Food Insecurity, Colleen Heflin, Irma Arteaga, Sara Gable

University of Kentucky Center for Poverty Research Discussion Paper Series

Rates of food insecurity in households with children have significantly increased over the past decade. The majority of children, including those at risk for food insecurity, participate in some form of non-parental child care during the preschool years. To evaluate the relationship between the two phenomenon, this study investigates the effects of child care arrangements on food insecurity in households with children. To address the selection bias problem that arises from the fact that enrollment in different types of child care is not a random process, this study uses propensity scores techniques. The authors compare outcomes across five child care …

Atrazine Exposure In Public Drinking Water And Preterm Birth, Jessica L. Rinsky, Claudia Hopenhayn, Vijay Golla, Steven R. Browning, Heather M. Bush

Epidemiology and Environmental Health Faculty Publications

Objectives

Approximately 13% of all births occur prior to 37 weeks gestation in the U.S. Some established risk factors exist for preterm birth, but the etiology remains largely unknown. Recent studies have suggested an association with environmental exposures. We examined the relationship between preterm birth and exposure to a commonly used herbicide, atrazine, in drinking water.

Methods

We reviewed Kentucky birth certificate data for 2004–2006 to collect duration of pregnancy and other individual-level covariates. We assessed existing data sources for atrazine levels in public drinking water for the years 2000–2008, classifying maternal county of residence into three atrazine exposure groups. …

Families With Hungry Children And The Transition From Preschool To Kindergarten, Colleen Heflin, Irma Arteaga, Sara Gable

University of Kentucky Center for Poverty Research Discussion Paper Series

This paper exploits a source of variation in the eligibility for federal nutrition programs to identify the program effects on food insecurity. Children are eligible for the WIC program until the day before they turn 61 months old. The result is an age discontinuity in program participation at the 61-month cutoff. Using the Early Childhood Longitudinal Study Birth-cohort dataset, we find strong evidence of a sizeable increase in household food insecurity at the 61- month cutoff. Our findings are robust to different model specifications, datasets, and various bandwidth choices using various non-parametric estimations.

A Rose By Any Other Statute Would Smell As Sweet: Patent Protection Of Ornamental Plants, Catherine Anne Barrett

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

Three Studies To Investigate Biopsychosocial Influences On Marital Conflict, Claire Kimberly

Theses and Dissertations--Family Sciences

Research is beginning to find a positive and significant relationship between marriage and health. Even though the current literature shows that separation and divorce have strong negative consequences for the mental and physical health of both spouses (Dush & Amato, 2005), the answer to why and how this occurs has yet to be solved.

A comprehensive perspective that could greatly benefit the analysis of this connection is the use of social neuroscientific methods in a biopsychosocial model. By including biological factors, social elements, and psychological variables in analyzing marriages, researchers would be able to further understand both the intra- and …

Influence Of The Neotyphodium--Tall Fescue Symbiosis On Belowground Processes, D. H. Mcnear Jr., Rebecca L. Mcculley

Plant and Soil Sciences Faculty Publications

Much of the work to date on the relationships between cool season grasses and Neotyphodium fungal endophytes has focused on the physiological, biochemical, and genetic ramifications of the host-fungus relationship and the subsequent influence these effects have on ruminant nutrition, plant adaptation to environmental stresses, and aboveground ecological processes. Relatively little attention has been paid to effects on belowground parameters. In this paper, we review the research evaluating the impact of one endophyte-grass association, the Neotyphodium – tall fescue symbiosis, on underground ecological and biogeochemical processes. We also present some preliminary data showing that the quantity and nature of tall …

Molecular And Biochemical Characterization Of Oleate- And Glycerol-3-Phosphate-Regulated Signaling In Plants, Mihir Kumar Mandal

Theses and Dissertations--Plant Pathology

Oleic acid (18:1), a monounsaturated fatty acid (FA), is synthesized upon desaturation of stearic acid (18:0) and this reaction is catalyzed by the plastidal enzyme stearoyl-acyl carrier protein-desaturase (SACPD). A mutation in the SSI2/FAB2 encoded SACPD lowers 18:1 levels, which correlates with induction of various resistance (R) genes and increased resistance to pathogens. Genetic and molecular studies have identified several suppressors of ssi2 which restore altered defense signaling either by normalizing 18:1 levels or by affecting function(s) of a downstream component. Characterization of one such ssi2 suppressor mutant showed that it is required downstream of low 18:1-mediated constitutive …

Creed Vs. Deed: Secession, Legitimacy, And The Use Of Child Soldiers, Trace C. Lasley

Theses and Dissertations--Political Science

The use of child soldiers has troubled human rights activists, policy-makers, and local communities for decades. Although rebellions around the world routinely use children in their activities, many do not. Despite its overwhelming importance for conflict resolution, the topic of child soldiers remains understudied. My research blends classic rational choice and constructivist themes to develop an explanation for when child soldiers will be used, and when they will be avoided.

The likelihood of child recruitment is influenced by the value of international opinion; this is determined by the groups' long-term goals. Secessionist rebellions desire to have their own state. However, …

Glimpses Of Ecstasy: The Public Shaping Of Personal History In Carlos Bulosan's America Is In The Heart, Nicholas A. Henson

disClosure: A Journal of Social Theory

No abstract provided.

Telling Stories From Haiti: Dany Laferriere And Authenticity And Authority In Autobiography, Lee Skallerup Bessette

disClosure: A Journal of Social Theory

No abstract provided.

Dismemberment In The Chicana/O Body Politic: Fragmenting NationNess And Form In Oscar Zeta Acosta's The Revolt Of The Cockroach People, And Alejandro Morales's The Rag Doll Plagues, Danizete Martinez

disClosure: A Journal of Social Theory

No abstract provided.

Wild Minds Searching: Early Scholars Groping In The Gap, Joy Denise Scott, Jane Grellier

disClosure: A Journal of Social Theory

No abstract provided.

Global Kentucky (Winter 2012), University Of Kentucky International Center

Global Kentucky

No abstract provided.

Economic Modeling & Optimization Of A Region Specific Multi-Feedstock Biorefinery Supply Chain, William H. Faulkner

Theses and Dissertations--Manufacturing Systems Engineering

The objective of this thesis is to include strategic and tactical level decisions into the biorefinery supply chain design for a specific region while comparing multiple conversion technologies and biomass feedstocks. The allocation of biomass feedstocks, products, and the respective supply chain configuration locations are determined while ensuring the regions monthly biomass availability and product market demand constraints are met. This research considers all actions required to bring the bio-based products to market from harvesting, storing, and processing the biomass to market distribution. Two different conversion technologies are chosen for comparison: one advanced conversion technology and one conventional technology. Potential …

Changes To The Equine Hindgut Microflora In Response To Antibiotic Challenge, Brittany E. Harlow

Theses and Dissertations--Animal and Food Sciences

Antibiotics are important to equine medicine, but can cause detrimental side-effects including reduced feed intake, allergic reactions, and diarrhea. Antibiotic-associated diarrhea (AAD) is attributed to disruption of the hindgut microflora, permitting proliferation of pathogenic microbes. The objectives were to evaluate the effects of antibiotics on beneficial fecal bacteria, AAD-associated pathogens, microbial species richness and fermentation. Horses were assigned to treatment groups: control (no antibiotics, n=6), trimethoprim-sulfadiazine (oral, n=6), or sodium ceftiofur (IM, n=6). Fecal samples were taken during adaptation (3 wk), antibiotic challenge (1 wk), and withdrawal (1 wk). Fecal cellulolytics decreased by >99% during challenge and did not recover …

Co-Grazing Beef Cattle And Goats In Kentucky, David C. Ditsch, Patty Scharko

Plant and Soil Sciences Research Report

A two season grazing study of mixed (goats and beef cattle) species was conducted in 2006 and 2008 at Sebastian Farms in Breathitt County KY. The objective of this study was to determine if the order of mixed species grazing affected beef cattle and goat weight gain and goat exposure to the barber pole worm (Haemonchus contortus) in a rotational grazing system. In this study, two co-grazing management strategies were tested. In treatment 1, goats and cattle were rotationally grazed together. In treatment 2, goats rotated through pastures as first grazers followed by cattle. Each co-grazing treatment was …

Clinical Evaluation Of Novel Methods For Extending Microneedle Pore Lifetime, Nicole K. Brogden

Theses and Dissertations--Pharmacy

Microneedles are a minimally invasive method for delivering drugs through the impermeable skin layers, and have been used to deliver a variety of compounds including macromolecules, vaccines, and naltrexone. Microneedles can be applied to the skin once, creating micropores that allow for drug delivery into the underlying circulation from a drug formulation. The utility of this technique, however, is blunted by rapid micropore closure. This research project sought to: 1) characterize micropore lifetime and re-sealing kinetics, and 2) prolong micropore lifetime via inhibition of the skin’s barrier restoration processes. Impedance spectroscopy was used as a surrogate technique in animals and …

Examining Chronic Non-Cancer Pain Among A Sample Of Individuals In Opioid Treatment Programs, Erin Stevenson

Theses and Dissertations--Social Work

National rates of chronic non-cancer pain (CNCP) are rising alongside increasing reports of prescription opioid abuse and mortality. Associations between the rise in CNCP and in opioid abuse seem logical, yet research on CNCP among individuals with opioid dependence is currently limited due to the complicated nature of comorbid conditions in research and treatment. This study aims to expand the CNCP knowledge base by responding to the question: Do individuals with CNCP participating in an opiate treatment program have better or worse treatment outcomes than individuals without CNCP?

This study used a secondary dataset including 483 adults from Kentucky’s Opiate …

Special Use Valuation Under The Internal Revenue Code: Does It Matter Now And Will It Matter In The Future?, Rusty Wade Rumley

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

Horse Racing Regulatory Reform Through Constructive Engagement By Industry Stakeholders With State Regulators, Alexander M. Waldrop, Karl M. Nobert, John W. Polonis

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

The Adverse Effects Of Aquatic Invasive Species On Native Commercial And Recreational Fisheries Of The Great Lakes And The Exacerbation Of The Problem By Judicial Reluctance To Act, Andrew S. Leung

Kentucky Journal of Equine, Agriculture, & Natural Resources Law

No abstract provided.

Characteristics Of All-Terrain Vehicles And Their Operators On Kentucky Farms, Jessica L. Wilson

Theses and Dissertations--Nursing

All-terrain vehicles (ATVs) were first developed and marketed in the U.S. in the 1970s. They have soared in popularity for occupational and recreational uses since that time. In 2008, there were approximately 10.2 million all-terrain vehicles (ATVs) in use in the United States. In 2001, it was estimated that 23 million Americans rode ATVs; 69% were adults and 31% children (CPSC, 2003). Deaths and injuries from ATVs have increased over time. According to the U.S. Consumer Product Safety Commission, Kentucky led the nation for ATV deaths from 2002-2006, with increasing numbers of fatalities annually.

ATV use on farms is increasing …