Digital Commons Network^™

Co2 Recycling Using Microalgae For The Production Of Fuels, Michael H. Wilson, John Groppo, Andrew Placido, S. Graham, S. A. Morton Iii, Eduardo Santillan-Jimenez, Aubrey Shea, Mark Crocker, Czarena Crofcheck, Rodney Andrews

Center for Applied Energy Research Faculty and Staff Publications

CO₂ capture and recycle using microalgae was demonstrated at a coal-fired power plant (Duke Energy’s East Bend Station, Kentucky). Using an in-house designed closed loop, vertical tube photobioreactor, Scenedesmus acutus was cultured using flue gas as the CO₂ source. Algae productivity of 39 g/(m² day) in June–July was achieved at significant scale (18,000 L), while average daily productivity slightly in excess of 10 g/(m² day) was demonstrated in the month of December. A protocol for low-cost algae harvesting and dewatering was developed, and the conversion of algal lipids—extracted from the harvested biomass—to diesel-range hydrocarbons via catalytic …

Redox-Mediated And Ionizing-Radiation-Induced Inflammatory Mediators In Prostate Cancer Development And Treatment, Lu Miao, Aaron K. Holley, Yanming Zhao, William H. St. Clair, Daret K. St. Clair

Toxicology and Cancer Biology Faculty Publications

SIGNIFICANCE: Radiation therapy is widely used for treatment of prostate cancer. Radiation can directly damage biologically important molecules; however, most effects of radiation-mediated cell killing are derived from the generated free radicals that alter cellular redox status. Multiple proinflammatory mediators can also influence redox status in irradiated cells and the surrounding microenvironment, thereby affecting prostate cancer progression and radiotherapy efficiency.

RECENT ADVANCES: Ionizing radiation (IR)-generated oxidative stress can regulate and be regulated by the production of proinflammatory mediators. Depending on the type and stage of the prostate cancer cells, these proinflammatory mediators may lead to different biological consequences ranging from …

Poster Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

1. Grading a Watershed? Patrick Creager, Eastern Kentucky University, Richmond, KY
2. Permeability Heterogeneity and Its Effects on Hyporheic Zone Exchange, Timothy Pryshlak and Audrey Sawyer, Department of Earth and Environmental Sciences, University of Kentucky, Lexington, KY
3. Effects of Atrazine on the Chemical Alarm Cue Response in Longear Sunfish (Lepomis megalottis), Ben M. Adams and Ben F. Brammell, Natural Sciences Department, Asbury University, Wilmore, KY
4. New Features Available on the Kentucky Groundwater Data Repository, Bart Davidson and others, Kentucky Geological Survey, University of Kentucky, Lexington, KY
5. Removal of Persistent Organic Pollutants from Contaminated Water Sources Utilizing Polyphenol- Functionalized Magnetic …

Session 2b, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Watershed-Scale Stable Isotope Simulation of the Fluvial Organic Carbon Budget Using the ISOFLOC Model, William Ford and Jimmy Fox, Department of Civil Engineering, University of Kentucky, Lexington, KY
• Time Average Velocity Characteristics of Decelerating Open Channel Flows, Robert Stewart and Jimmy Fox, Department of Civil Engineering, University of Kentucky, Lexington, KY
• Spatiotemporal Relevance of Soil Moisture's Interaction with Measured Soil-Terrain Parameters, Carla Landrum and others, Lexington, KY
• Transport of Agricultural Contaminants through a Karst Soil, Carl Bolster and others, Food Animal Environmental Research Unit, USDA-ARS, Bowling Green, KY

Plenary Session, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

• Using Microbial-Source Tracking to Investigate Sources of Fecal Contamination in the South Fork Little River Basin, Hopkinsville, Kentucky, Angie Crain and Mac Cherry, USGS Kentucky Water Science Center, Louisville, KY
• Application of Nitrogen and Oxygen Isotopes in Tracing Nitrate Sources in the South Fork Little River Basin, Hopkinsville, Kentucky, Angie Crain and Mac Cherry, USGS Kentucky Water Science Center, Louisville, KY
• Kentucky StreamStats Water-Resources Web Application: Enhancements and Implementation of Selected Low- and Mean-Flow Estimating Equations, Gary Martin, USGS Kentucky Water Science Center, Louisville, KY
• Kentucky Division of Water's Risk Communication Toolbox, Annemarie Cason and Kristen …

Proceedings Of 2014 Kentucky Water Resources Annual Symposium, Kentucky Water Resources Research Institute, University Of Kentucky

Kentucky Water Resources Annual Symposium

This symposium was planned and conducted as a part of the state water resources research institute annual program that is supported by Grant/Cooperative Agreement Number G11AP20081 from the United States Geological Survey. The contents of this proceedings document and the views and conclusions presented at the symposium are solely the responsibility of the individual authors and presenters and do not necessarily represent the official views of the USGS or of the symposium organizers and sponsors. This publication is produced with the understanding that the United States Government is authorized to reproduce and distribute reprints for government purposes.

Redox-Modulated Phenomena And Radiation Therapy: The Central Role Of Superoxide Dismutases, Aaron K. Holley, Lu Miao, Yanming Zhao, Daret K. St. Clair, William H. St. Clair

Toxicology and Cancer Biology Faculty Publications

SIGNIFICANCE: Ionizing radiation is a vital component in the oncologist's arsenal for the treatment of cancer. Approximately 50% of all cancer patients will receive some form of radiation therapy as part of their treatment regimen. DNA is considered the major cellular target of ionizing radiation and can be damaged directly by radiation or indirectly through reactive oxygen species (ROS) formed from the radiolysis of water, enzyme-mediated ROS production, and ROS resulting from altered aerobic metabolism.

RECENT ADVANCES: ROS are produced as a byproduct of oxygen metabolism, and superoxide dismutases (SODs) are the chief scavengers. ROS contribute to the radioresponsiveness of …

The Soldiers In Societies: Defense, Regulation, And Evolution, Li Tian, Xuguo Zhou

Entomology Faculty Publications

The presence of reproductively altruistic castes is one of the primary traits of the eusocial societies. Adaptation and regulation of the sterile caste, to a certain extent, drives the evolution of eusociality. Depending on adaptive functions of the first evolved sterile caste, eusocial societies can be categorized into the worker-first and soldier-first lineages, respectively. The former is marked by a worker caste as the first evolved altruistic caste, whose primary function is housekeeping, and the latter is highlighted by a sterile soldier caste as the first evolved altruistic caste, whose task is predominantly colony defense. The apparent functional differences between …

Systems And Methods For Diagnosis And Monitoring Of Bacteria-Related Conditions, Sylvia Daunert, Sapna K. Deo, Patrizia Pacini, Anjali Kumari Struss, Harohalli Shashidhar, Deborah R. Auer Flomenhoft, Nilesh Raut

Chemistry Faculty Patents

The presently-disclosed subject matter provides systems, methods, and kits for diagnosing and/or monitoring a bacteria-related condition of interest in a subject by providing a cell sensing system, each system containing a reporter molecule capable of detecting binding of a quorum sensing molecule and capable of generating a detectable signal.

Soy Products With Reduced Levels Of Sulfite, Free Radicals And Methanethiol, William L. Boatright

Animal and Food Sciences Faculty Patents

Soy products or compositions are treated with a food grade iodate compound or a cystine compound to reduce levels of methanethiol, sulfites and sulfite free-radicals, sulfate free radicals and other free radicals generated from sulfite free radicals in the soy products or compositions by 1% to 95%.

Who Is Most Vulnerable To Social Rejection? The Toxic Combination Of Low Self-Esteem And Lack Of Negative Emotion Differentiation On Neural Responses To Rejection, Todd B. Kashdan, C. Nathan Dewall, Carrie L. Masten, Richard S. Pond Jr., Caitlin Powell, David Combs, David R. Schurtz, Antonina S. Farmer

Psychology Faculty Publications

People have a fundamental need to belong that, when satisfied, is associated with mental and physical well-being. The current investigation examined what happens when the need to belong is thwarted—and how individual differences in self-esteem and emotion differentiation modulate neural responses to social rejection. We hypothesized that low self-esteem would predict heightened activation in distress-related neural responses during a social rejection manipulation, but that this relationship would be moderated by negative emotion differentiation—defined as adeptness at using discrete negative emotion categories to capture one's felt experience. Combining daily diary and neuroimaging methodologies, the current study showed that low self-esteem and …

How To Make A Country Ham, Gregg Rentfrow, Surendranath Suman

Agriculture and Natural Resources Publications

Country ham is the dry-cured hind leg of a pig that harks back to the way food was preserved before mechanical refrigeration. The country ham finds its roots in China and European dry-cured hams such as China’s Jinhua and Yunnan hams, Italy’s Prosciutto, Spain’s Serrano and Iberian hams, and Germany’s Black Forest ham. Country hams are drastically different from their more common cousin, the wet-cured ham, also known as “city ham.” The flavor of a country ham is complex, and the texture is unlike that of other hams.

A Fresh Cow Health Monitoring System, Amanda E. Sterrett, Donna M. Amaral-Phillips, Jeffrey M. Bewley, Michelle Arnold

Agriculture and Natural Resources Publications

Calving causes cows to endure many physiological changes that require good nutrition, time, and adequate rest for recovery. Upon calving, cows are introduced into the lactating herd, where they consume high amounts of a new ration and have to adapt to a new feeding and milking routine. Lactation requires more support from the diet than pregnancy and weight maintenance, particularly in fresh cows calving in with a negative energy balance. Additionally, the social hierarchy within the herd is re-established every time a new cow enters the group, which can cause stress, especially for timid and weak cows. For these reasons, …

Nitrate Poisoning, Michelle Arnold, Cynthia Gaskill, Jeff Lehmkuhler, S. Ray Smith

Agriculture and Natural Resources Publications

Nitrates are present in all plants, but normally their concentrations are not excessive. Under normal growing conditions, nitrate from the soil is absorbed by the roots of forage plants, and is supplied to the upper portions of the plant (primarily leaves) where it is converted into plant protein. However, adverse environmental conditions (such as drought), sudden weather changes (cool, cloudy weather), leaf damage (due to hail, frost, or herbicides), or heavy fertilization with nitrogen, can cause plants to develop and retain potentially dangerous levels of nitrate. The lower stalks and stems at the base of the plant are the site …

Stereotypic Behavior In Horses: Weaving, Stall Walking And Cribbing, Fernanda Camargo

Agriculture and Natural Resources Publications

Many stabled horses perform a variety of repetitive behaviors such as weaving, stall walking, cribbing, headshaking and pawing. These behaviors have been called many different names including stereotypic behavior, stereotypies, stereotypes, obsessive compulsive disorders, vices and habits. Although it may be difficult to know why exactly each horse performs these vices, there may be specific causal factors for these activities in the horse. These behaviors are not simply learned and not simply inherited, but may be a mixture of both. Studies show that some families of horses have a higher prevalence of certain vices, which suggests heritability and genetic components. …

Sex Differences In A Mouse Model Of Multiple Sclerosis: Neuropathic Pain Behavior In Females But Not Males And Protection From Neurological Deficits During Proestrus, Elizabeth J. Rahn, Tommaso Iannitti, Renee R. Donahue, Bradley K. Taylor

Physiology Faculty Publications

BACKGROUND: Multiple sclerosis (MS), a demyelinating disease of the central nervous system, is one of the most prevalent neurological disorders in the industrialized world. This disease afflicts more than two million people worldwide, over two thirds of which are women. MS is typically diagnosed between the ages of 20-40 and can produce debilitating neurological impairments including muscle spasticity, muscle paralysis, and chronic pain. Despite the large sex disparity in MS prevalence, clinical and basic research investigations of how sex and estrous cycle impact development, duration, and severity of neurological impairments and pain symptoms are limited. To help address these questions, …

Spatiotemporal Expression Of Manf In The Developing Rat Brain, Haiping Wang, Zunji Ke, Alexander Alimov, Mei Xu, Jacqueline A. Frank, Shengyun Fang, Jia Luo

Pharmacology and Nutritional Sciences Faculty Publications

Mesencephalic astrocyte-derived neurotrophic factor (MANF) is an evolutionarily conserved neurotrophic factor which exhibited neuroprotective properties. Recent studies suggested that MANF may play a role in the neural development of Drosophila and zebra fishes. In this study, we investigated the spatiotemporal expression of MANF in the brain of postnatal and adult rats. MANF expression appeared wide spread and mainly localized in neurons. In the cerebral cortex, neurons in layer IV and VI displayed particularly strong MANF immunoreactivity. In the hippocampus, intensive MANF expression was observed throughout the subfields of Cornu Amonis (CA1, CA2, and CA3) and the granular layer of the …

Anti-Lysophosphatidic Acid Antibodies Improve Traumatic Brain Injury Outcomes, Peter J. Crack, Moses Zhang, Maria Cristina Morganti-Kossmann, Andrew J. Morris, Jonathan M. Wojciak, Jonathan K. Fleming, Ila Karve, David Wright, Maithili Sashindranath, Yona Goldshmit, Alison Conquest, Maria Daglas, Leigh A. Johnston, Robert L. Medcalf, Roger A. Sabbadini, Alice Pébay

Internal Medicine Faculty Publications

BACKGROUND: Lysophosphatidic acid (LPA) is a bioactive phospholipid with a potentially causative role in neurotrauma. Blocking LPA signaling with the LPA-directed monoclonal antibody B3/Lpathomab is neuroprotective in the mouse spinal cord following injury.

FINDINGS: Here we investigated the use of this agent in treatment of secondary brain damage consequent to traumatic brain injury (TBI). LPA was elevated in cerebrospinal fluid (CSF) of patients with TBI compared to controls. LPA levels were also elevated in a mouse controlled cortical impact (CCI) model of TBI and B3 significantly reduced lesion volume by both histological and MRI assessments. Diminished tissue damage coincided with …

Nuclear Interaction Between Adr-Induced P65 And P53 Mediates Cardiac Injury In Inos (−/−) Mice, Marsha P. Cole, Jitbanjong Tangpong, Terry D. Oberley, Luksana Chaiswing, Kinsley K. Kiningham, Daret K. St. Clair

Toxicology and Cancer Biology Faculty Publications

Adriamycin (ADR) treatment causes an imbalance in the levels of nitric oxide (^•NO) and superoxide (O₂^•−) production leading to cardiac injury. Previously we demonstrated that mice lacking inducible nitric oxide synthase (iNOS) have increased oxidative stress and mitochondrial injury. The molecular events leading to increased mitochondrial injury in iNOS deficient mice is unknown. ADR in the absence of iNOS preferentially activates a proapoptotic pathway without a concurrent increase in prosurvival pathways. Treatment with ADR leads to an increase in DNA binding activity of nuclear factor kappa B (NFκB) and p53 in wildtype mice. Following ADR …

Alfalfa For Summer Grazing, Roy Burris, Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

Alfalfa is most commonly used as a hay crop in the United States. However, with proper management, it can be utilized as a grazing crop with very good results. Liveweight gains per acre are quite high for grazing beef cattle with total season grains of 500 to 800 pounds per acre being reasonable. The authors realized 732 lb/acre of gains on 16 acres of alfalfa in 1991, with beef steers at Princeton.

Lessons Learned In Australia--Uk Forages Study Tour, Traci Missun, S. Ray Smith, Garry D. Lacefield

Kentucky Alfalfa and Stored Forage Conference

In September 2013, twenty Kentuckians traveled to Australia to tour farms and attend the International Grasslands Congress. The group included ten county agents, five farmers, four UK faculty/staff and a teacher. This study tour allowed participants to gain a new perspective of agriculture and how Australian farmers deal with adversity. Farmers there face many challenges each year with change in precipitation, ranging from severe drought to flooding. These challenges have forced them to incorporate different methods to be successful, including intensive grazing, use of overhead and drip irrigation for pasture and hay production, and fallow farming.

Alfalfa Baleage/Silage Preservatives: Do They Work? Are They Economical?, S. Ray Smith

Kentucky Alfalfa and Stored Forage Conference

There are many variables with silage production including - type of silage operation (silo, bunk, tube, or wrapped bale), weather, packing or bale density, speed of covering. Therefore, no two silage crops are ever the same, which is why researchers are reluctant to give blanket statements regarding when and how to use silage inoculants. There has been a lot of good research though in recent years about the effect of silage inoculants under various management and conditions. What follows is an overview of this research and some general recommendations. Much of this overview was taken from a very good fact …

Inactivation Of The Host Lipin Gene Accelerates Rna Virus Replication Through Viral Exploitation Of The Expanded Endoplasmic Reticulum Membrane, Chingkai Chuang, Daniel Barajas, Jun Qin, Peter D. Nagy

Plant Pathology Faculty Publications

RNA viruses take advantage of cellular resources, such as membranes and lipids, to assemble viral replicase complexes (VRCs) that drive viral replication. The host lipins (phosphatidate phosphatases) are particularly interesting because these proteins play key roles in cellular decisions about membrane biogenesis versus lipid storage. Therefore, we examined the relationship between host lipins and tombusviruses, based on yeast model host. We show that deletion of PAH1 (phosphatidic acid phosphohydrolase), which is the single yeast homolog of the lipin gene family of phosphatidate phosphatases, whose inactivation is responsible for proliferation and expansion of the endoplasmic reticulum (ER) membrane, facilitates robust RNA …

Nuclear Receptor 4a3 (Nr4a3) Regulates Murine Mast Cell Responses And Granule Content, Gianni Garcia-Faroldi, Fabio R. Melo, Dennis Bruemmer, Orla M Conneely, Gunnar Pejler, Anders Lundequist

Internal Medicine Faculty Publications

Nuclear receptor 4a3 (Nr4a3) is a transcription factor implicated in various settings such as vascular biology and inflammation. We have recently shown that mast cells dramatically upregulate Nuclear receptor 4a3 upon activation, and here we investigated the functional impact of Nuclear receptor 4a3 on mast cell responses. We show that Nuclear receptor 4a3 is involved in the regulation of cytokine/chemokine secretion in mast cells following activation via the high affinity IgE receptor. Moreover, Nuclear receptor 4a3 negatively affects the transcript and protein levels of mast cell tryptase as well as the mast cell’s responsiveness to allergen. Together, these findings identify …

B Cell Tolerance In Health And Disease, Murali Gururajan, Vishal J. Sindhava, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

B lymphocyte receptors are generated randomly during the bone marrow developmental phase of B cells. Hence, the B cell repertoire consists of both self and foreign antigen specificities necessitating specific tolerance mechanisms to eliminate self-reactive B cells. This review summarizes the major mechanisms of B cell tolerance, which include clonal deletion, anergy and receptor editing. In the bone marrow presentation of antigen in membrane bound form is more effective than soluble form and the role of dendritic cells in this process is discussed. Toll like receptor derived signals affect activation of B cells by certain ligands such as nucleic acids …

Bis-Quaternary Ammonium Cyclophane Compounds That Interact With Neuronal Nicotinic Acetylcholine Receptors, Peter A. Crooks, Linda P. Dwoskin, Guangrong Zheng, Sangeetha Sumithran, Davis D. Allen, Zhenfa Zheng, Paul Lockman

Pharmaceutical Sciences Faculty Patents

Provided are bis-quaternary ammonium cyclophane compounds which are modulators of nicotinic acetylcholine receptors. Also provided are methods of using the compounds for modulating the function of a nicotinic acetylcholine receptor, and for the prevention and/or treatment of central nervous system disorders, substance use and/or abuse, and or gastrointestinal tract disorders.

Enhancing Transdermal Delivery Of Opioid Antagonists And Agonists Using Codrugs Linked To Bupropion Or Hydroxybupropion, Audra L. Stinchcomb, Peter A. Crooks, Mohamad O. Hamad, Paul K. Kiptoo

Pharmaceutical Sciences Faculty Patents

The present invention is directed to novel codrugs comprising bupropion or hydroxybupropion and an opioid antagonist or an opioid agonist joined together by chemical bonding. The codrugs provide a significant increase in the transdermal flux across human skin, as compared to the basic opioid antagonist or opioid agonist.

Proteoglycans: Road Signs For Neurite Outgrowth, Justin A. Beller, Diane M. Snow

Spinal Cord and Brain Injury Research Center Faculty Publications

Proteoglycans in the central nervous system play integral roles as "traffic signals" for the direction of neurite outgrowth. This attribute of proteoglycans is a major factor in regeneration of the injured central nervous system. In this review, the structures of proteoglycans and the evidence suggesting their involvement in the response following spinal cord injury are presented. The review further describes the methods routinely used to determine the effect proteoglycans have on neurite outgrowth. The effects of proteoglycans on neurite outgrowth are not completely understood as there is disagreement on what component of the molecule is interacting with growing neurites and …

Response Assessment Of Novottf-100a Versus Best Physician's Choice Chemotherapy In Recurrent Glioblastoma, Eric T. Wong, Edwin Lok, Kenneth D. Swanson, Shiva Gautam, Herbert H. Engelhard, Frank Lieberman, Sophie Taillibert, Zvi Ram, John L. Villano

Markey Cancer Center Faculty Publications

The NovoTTF-100A device emits frequency-tuned alternating electric fields that interfere with tumor cell mitosis. In phase III trial for recurrent glioblastomas, NovoTTF-100A was shown to have equivalent efficacy and less toxicity when compared to Best Physician's Choice (BPC) chemotherapy. We analyzed the characteristics of responders and nonresponders in both cohorts to determine the characteristics of response and potential predictive factors. Tumor response and progression were determined by Macdonald criteria. Time to response, response duration, progression-free survival (PFS) ± Simon-Makuch correction, overall survival (OS), prognostic factors, and relative hazard rates were compared between responders and nonresponders. Median response duration was 7.3 …

Aged Rats Are Hypo-Responsive To Acute Restraint: Implications For Psychosocial Stress In Aging, Heather M. Buechel, Jelena Popovic, Kendra Staggs, Katie L. Anderson, Olivier Thibault, Eric M. Blalock

Pharmacology and Nutritional Sciences Faculty Publications

Cognitive processes associated with prefrontal cortex and hippocampus decline with age and are vulnerable to disruption by stress. The stress/stress hormone/allostatic load hypotheses of brain aging posit that brain aging, at least in part, is the manifestation of life-long stress exposure. In addition, as humans age, there is a profound increase in the incidence of new onset stressors, many of which are psychosocial (e.g., loss of job, death of spouse, social isolation), and aged humans are well-understood to be more vulnerable to the negative consequences of such new-onset chronic psychosocial stress events. However, the mechanistic underpinnings of this age-related shift …