Digital Commons Network^™

Foster Parents' Perceptions And Beliefs About Social Emotional Development In Infants And Toddlers, Susan R. Noel

DNP Projects

Infants and toddlers placed in foster care are at increased risk for social-emotional (SE) development dysregulation. Foster parents, as surrogate parents, play a critical role in a young child having the potential for healthy SE development. The transactional model supports the complex and intricate interplays and transactions of the child, the foster parent, and the environment that influences a healthy SE development. This qualitative descriptive study explored foster parent knowledge, behavioral cue recognitions, and role perception regarding a young child's SE development. Although the sample size was small, the study provides relevant information, from the on-going experiences of foster parents, …

Conservation Agriculture In Kentucky: Investigating Nitrogen Loss And Dynamics In Corn Systems Following Wheat And Hairy Vetch Cover Crops, Rebecca Erin Shelton

Theses and Dissertations--Plant and Soil Sciences

Unintentional nitrogen (N) loss from agroecosystems produces greenhouse gases, induces eutrophication, and is costly for farmers; therefore, adoption of conservation agricultural management practices, such as no-till and cover cropping, has increased. This study assessed N loss via leaching, NH₃ volatilization, N₂O emissions, and N retention in plant and soil pools of corn conservation agroecosystems across a year. Three systems were evaluated: 1) an unfertilized organic system with cover crops Vicia villosa, Triticum aestivum, or a mix of the two; 2) an organic system with a Vicia cover crop employing three fertilization schemes (0 N, organic N, …

Soil Management And Nitrogen Dynamics In Burley Tobacco Rotations, Congming Zou

Theses and Dissertations--Plant and Soil Sciences

Agronomic practices, including tillage, crop rotation and N fertilization, have been developed to efficiently manage soil N dynamics and crop N nutrition. These practices can affect soil organic carbon (SOC) and soil total nitrogen (STN) sequestration, and consequently influence soil nitrogen mineralization (SNM) and crop N nutrition. However, little research has been systematically and simultaneously conducted to examine the effect of agronomic management on (1) SOC and STN stocks; (2) SNM; and (3) crop N nutrition. Burley tobacco (Nicotiana tobacum L.) is a N demanding crop and subject to inefficiency in N fertilization. Moreover, conservation tillage and rotation have been …

Unveiling Novel Aspects Of D-Amino Acid Metabolism In The Model Bacterium Pseudomonas Putida Kt2440, Atanas D. Radkov

Theses and Dissertations--Plant and Soil Sciences

D-amino acids (D-AAs) are the α-carbon enantiomers of L-amino acids (L- AAs), the building blocks of proteins in known organisms. It was largely believed that D-AAs are unnatural and must be toxic to most organisms, as they would compete with the L-counterparts for protein synthesis. Recently, new methods have been developed that allow scientists to chromatographically separate the two AA stereoisomers. Since that time, it has been discovered that D-AAs are vital molecules and they have been detected in many organisms. The work of this dissertation focuses on their place in bacterial metabolism. This specific area was selected due to …

Urea Formulations On The Productivity Of Bermudagrass And Bermudagrass-White Clover Pastures, Caitlin Timberlake

Theses and Dissertations--Plant and Soil Sciences

Bermudagrass (Cynodon dactylon L.) is a perennial warm-season grass that is very responsive to nitrogen (N) fertilization. Excessive N applications have negative environmental consequences and make maintaining mixed swards difficult. This study determined the effects of enhanced efficiency (EE) N fertilizers and fertilizer rate on bermudagrass yields, nutritive values, and white clover persistence. Nitrogen sources included urea, urea formulated with Agrotain^® (U+A), urea with Agrotain^® and dicyandiamide (SuperU), a polymer-coated urea (ESN), ESN+urea (75% ESN, 25% urea), and methylene urea (MU). In the urea formulation trial, SuperU and U+A maximized forage yields at lower N rates. The …

Proteomics And Phosphoproteomics Of Brain: (1) Analyses Of The Pink1 Knockout Model Of Parkinson Disease; (2) Insights Into The Progression Of Alzheimer Disease; And (3) The Naked Mole-Rat Model Of Salubrious Aging, Judy C. Triplett

Theses and Dissertations--Chemistry

Proteomics is the field of science in which proteins produced by an organism (the proteome) are identified. The level of a particular protein can vary with time and the influence of cellular stressors. The study of phosphoproteomics is vital because tyrosine, serine and threonine phosphorylation modulate protein structure and function and is a crucial regulator of cellular signaling pathways. Dysregulation of protein and phosphorylation levels has been reported in multiple neurodegenerative disorders including Parkinson disease (PD) and Alzheimer disease (AD).

PINK1 is a mitochondrial serine/threonine kinase that polices mitochondrial integrity. Mutations in this protein are associated with familial early-onset PD. …

Thermal Degradation Of Amines For Co2 Capture, Quanzhen Huang

Theses and Dissertations--Chemistry

In the selection of candidates for CO₂ absorption, solvent thermal degradation has become a general concern due to the significant impact on operational cost and the intention to use thermal compression from high temperature stripping to minimize the overall process energy. In this research, the impact of flue gas contaminants on Monoethanolamine (MEA) thermal degradation was investigated at elevated temperatures consistent with those in the CO₂ stripper. Nitrite, fly ash, sulfate and thiosulfate were each added to 5.0 M MEA and the contaminant-containing MEA solutions were degraded at 125 °C, 135 °C and 145 °C. MEA degrades significantly …

Nanoindentation Of A Zinc Metal Soap Mixture For Use In A Laser Printer, George A. Nimick

Theses and Dissertations--Chemical and Materials Engineering

At the start of this project, the possible choices of metal soaps had already been narrowed to include some of the zinc soaps used in this project. These zinc soaps are mixtures of zinc stearate and zinc palmitate of varying ratios purchased from a supplier. Zinc soap was chosen as result of its common use in various industries as a lubricant and mold release, which implied potential benefits in an electrophotographic printing system. These potential benefits include, but are not limited to, a more efficient transfer from a photoconductive drum and protection of the drum from mechanical and chemical degradation. …

Determining The Effects Of Cd151 And Β1 On Tumor Cell Adhesion And Migration, Rachel R. Essex

Theses and Dissertations--Chemical and Materials Engineering

Previous studies have shown that the upregulation of CD151 and β₁ is associated with poor prognosis in many cancers such as breast cancer. Studies have provided evidence that these proteins are associated with the adhesion and migration of tumor cells. In this study, a microfluidic flow chamber was utilized to determine how CD151 and β₁ affected the firm and initial adhesion of metastatic breast cancer cells to a planar endothelial monolayer under shear stress. This system mimicked the adhesion of metastatic breast cancer cells to the endothelial cells of the circulatory system. CD151 and β₁ increased the …

Cocaine-Induced Behavioral Sensitization And Conditioned Place Preference In Japanese Quail (Coturnix Japonica): A Focus On Sex Differences And Dopaminergic Mechanisms, Karin E. Gill

Theses and Dissertations--Psychology

Research has indicated that gonadal hormones may mediate behavioral and biological responses to cocaine. Estrogen, in particular, has been shown to increase behavioral responding to cocaine in female rats relative to male rats. The use of Japanese quail may add to our knowledge of sex differences in drug abuse because of their advanced visual system and the ability to control their gonadal hormones via alterations in photoperiod. In three experiments, cocaine-induced behaviors were examined using this avian model.

In Experiment 1, I investigated the potential sex differences in cocaine-induced locomotor activity between male and female Japanese quail and I examined …

Delicious Justice: Schadenfreude Toward Atheists Bound For Hell, Maxine Najle

Theses and Dissertations--Psychology

In the wake of the death of a prominent atheist figure in 2011, an especially unsavory side of anti-atheist prejudice became evident as many celebrated the death of a prominent atheist, rejoicing that he would be in hell. The current study explores how these attitudes reveal a sense of schadenfreude in anti-atheist prejudice previously unexplored in the literature. Potential origins of this schadenfreude are discussed, and a study to experimentally explore this phenomenon was carried out. Using the repeated taste-test paradigm, this study gave participants atheist primes and hell primes between identical drinks and measure perceived taste after these manipulations, …

Development Of An Avian Model For Identifying Individual Differences In Drug Vulnerability, Beth A. Rice

Theses and Dissertations--Psychology

The attribution of incentive salience to cues that become associated with drugs of abuse is a critical characteristic of individuals who may be vulnerable to drug addiction. Rodents with the propensity to sign track are thought to be vulnerable to drug abuse. The goal of the current work was to investigate whether sign trackers (STs) would acquire cocaine conditioned place preference (CPP) to a discrete cue using an avian species. In Experiment 1, sign and goal trackers (GTs) were first identified using a one third rank order split. Following identification, cocaine-CPP was conducted with a discrete cue in each end …

The Five Factor Forgiveness Inventory: A Measure Of Forgiveness From The Perspective Of The Five Factor Model, Natalie G. Glover

Theses and Dissertations--Psychology

The purpose of this study was to develop and validate a five factor model (FFM) measure of forgiveness. Participants were 545 undergraduates currently enrolled in introductory and experimental psychology courses at the University of Kentucky (272 used for item construction, 273 for scale validation). Items were selected on the basis of convergent validity with five-factor model personality scales and forgiveness scales. Participants in the validation stage were administered the resulting Five Factor Forgiveness Inventory (FFFI), as well as the International Personality Item Pool-NEO (IPIP-NEO) and seven other existing forgiveness measures. Significant convergent validity was obtained for the vast majority of …

Gαq-Associated Signaling Promotes Neuroadaptation To Ethanol And Withdrawal-Associated Hippocampal Damage, Anna R. Reynolds

Theses and Dissertations--Psychology

Prolonged, heavy consumption of alcohol produces marked neuroadaptations in excitatory neurotransmission. These effects are accelerated following patterns of intermittent heavy drinking wherein periods of heavy consumption are followed by periods of abstinence. Studies have shown that neuroadaptive changes in the glutamatergic N-methyl-D-aspartate (NMDA) receptor produces excitotoxicity during periods of withdrawal; however, upstream targets were not adequately characterized. The present studies sought to identify these targets by assessing the role of group 1 metabotropic glutamate receptors (mGluR) and intracellular calcium in promoting cytotoxicity of hippocampal cell layers in vitro. It was hypothesized that ethanol-induced activity of mGluR1-and-5 contributes to hippocampal …

Effect Of Social Peers On Risky Decision Making In Male Sprague Dawley Rats, Virginia Weiss

Theses and Dissertations--Psychology

Adolescence is a time associated with increased risk taking and peer relations. Research has shown that adolescents are more vulnerable to peer pressure compared to adults, leading to exacerbated risk taking. Preclinical research suggests that these findings may also be applicable to adolescent rodents, which find social interaction rewarding and are prone to risky behavior. There is, however, little research on the effect of social interaction on rodent models of risky decision-making. This thesis utilized social chambers, which consisted of adjacent operant chambers separated by wire mesh. Adolescent rats performed a risky decision-making task in which they had a choice …

On A(Pe)Theism: Religious Dehumanization Of Atheists And Other Outgroups, Ben Kok Leong Ng

Theses and Dissertations--Psychology

Research on the dark side of religion has recently found evidence that anti-atheist prejudice is embedded in distrust (Gervais et al, 2011). Anti-atheist prejudice though old in its form, has only been systemically researched on over the last couple of years. This study seeks to extend on research in anti-atheist prejudice by examining religious dehumanization of atheists in comparison with other religious outgroups – gays and Muslims. Study 1 utilized a two factor model of dehumanization (Haslam, 2006) to examine dehumanization. Study 2 serves as a conceptual replication and extension using two different measures of dehumanization. Study 1 failed to …

The Effects Of Alcohol And Hypoxia Exposure In The Developing Brain, Megan L. Carter

Theses and Dissertations--Psychology

Exposure to alcohol (ethanol (ETOH)) during fetal development results in a variety of cognitive/behavioral deficits. The effects of fetal ETOH exposure vary across individuals and numerous studies have shown that ETOH’s interaction with other variables can affect outcome in offspring. These studies investigated the effects of developmental ETOH and hypoxia. It was hypothesized that a history of ETOH during development alters the response to a subsequent hypoxic challenge, such as that which may occur during parturition; this results in central nervous system (CNS) damage and behavioral deficits.

The first study determined if developmental ETOH and hypoxia exposure produced behavioral deficits …

The Use Of A Digital Game-Based Safety Program For All-Terrain Vehicles: Operational Knowledge For Youthful Riders, Mark Schneider

Theses and Dissertations--Curriculum and Instruction

Given the exposure and preference of digital natives for games, along with the proliferation of digital devices, there is a need to determine how effective digital games are in conveying operational knowledge to our youth. This quasi-experimental study examined whether a digital safety game increased high school student operational knowledge of All-terrain Vehicle (ATV) safety in contrast to conventional PowerPoint ATV safety training with commensurate information. The first treatment group consisted of “in class” students participating in the ATV game, the second treatment group was made up of “free play” students playing the game out of class, and finally the …

Muscular And Neural Contributions To Postactivation Potentiation, Brian Joseph Wallace

Theses and Dissertations--Kinesiology and Health Promotion

Muscle performance is partially a consequence of its recent contractile history. Postactivation potentiation (PAP) can occur after muscle contractions and leads to enhanced neuromuscular performance. The purpose of this dissertation was to explain the relationship between muscle factors (twitch potentiation, TP) and neural factors (reflex potentiation, RP) contributing to overall PAP following a non-fatiguing volitional muscle contraction. The tibial nerves of fifteen resistance trained volunteers (eleven men, four women) were stimulated intermittently at supramaximal (Mmax) and submaximal (Hmax) intensities for 20 minutes on separate days under three conditions: rest (Control); after a after a 10 second maximum voluntary isometric contraction …

Cardiovascular Disease Risk Factors In Structural Firefighters, Nicholas W. Trubee

Theses and Dissertations--Kinesiology and Health Promotion

This dissertation is composed of two manuscripts assessing cardiovascular disease (CVD) risk in structural firefighters. Study 1 compared traditional CVD risk factors and health-related behaviors between professional and volunteer firefighters. Online questionnaires were sent to approximately 4000 firefighters in the state of Kentucky. The results indicated that 90% of volunteer and 58% of professional firefighters were classified as moderate-to-high CVD risk. Volunteer firefighters were significantly (p < 0.001) older and more likely (p = 0.026) to be current cigarette smokers. The mean body mass index among all firefighters in the sample was 30.8 kg·m^-2. Nearly 60% of volunteer firefighters were obese. Obese firefighters were more likely (p < 0.05) to have been diagnosed as diabetic or pre-diabetes compared to overweight and normal weight firefighters. These results indicate that volunteer firefighters may be at a greater CVD risk compared to professional firefighters.

Study 2 investigated the association of cardiorespiratory fitness (CRF) and body fat with arterial stiffness in professional firefighters. Forty-six …

The Effect Of Fluid Periodization On Athletic Performance Outcomes In American Football Players, Christopher W. Morris

Theses and Dissertations--Kinesiology and Health Promotion

For decades strength and conditioning professionals have been seeking optimal training volumes and intensities to yield maximum performance outcomes without the onset of injury. Unfortunately, current studies apply experimental training techniques without considering the individuals’ response to the imposed training load. Due to the vast genetic variability and extraneous environmental factors that affect one’s ability to recover, results from such studies are controversial and inconclusive. Athlete monitoring systems offer an objective assessment that is purported to evaluate an individual’s physiological readiness to adapt to an overload stimulus and thus allow for daily manipulations in training loads (i.e., fluid periodization). However, …

A New Cecal Slurry Preparation Protocol With Improved Long-Term Reproducibility For Animal Models Of Sepsis, Marlene E. Starr, Allison M. Steele, Mizuki Saito, Bill J. Hacker, B. Mark Evers, Hiroshi Saito

Surgery Faculty Publications

Sepsis, a life-threatening systemic inflammatory response syndrome induced by infection, is widely studied using laboratory animal models. While cecal-ligation and puncture (CLP) is considered the gold standard model for sepsis research, it may not be preferable for experiments comparing animals of different size or under different dietary regimens. By comparing cecum size, shape, and cecal content characteristics in mice under different experimental conditions (aging, diabetes, pancreatitis), we show that cecum variability could be problematic for some CLP experiments. The cecal slurry (CS) injection model, in which the cecal contents of a laboratory animal are injected intraperitoneally to other animals, is …

Mtorc2 Is Required For Rit-Mediated Oxidative Stress Resistance, Weikang Cai, Douglas A. Andres

Molecular and Cellular Biochemistry Faculty Publications

Rit, a member of the Ras family of GTPases, has been shown to promote cell survival in response to oxidative stress, in part by directing an evolutionarily conserved p38 MAPK-Akt survival cascade. Aberrant Rit signaling has recently been implicated as a driver mutation in human cancer, adding importance to the characterization of critical Rit effector pathways. However, the mechanism by which Rit-p38 signaling regulated Akt activity was unknown. Here, we identify mTORC2 as a critical downstream mediator of Rit-dependent survival signaling in response to reactive oxygen species (ROS) stress. Rit interacts with Sin1 (MAPKAP1), and Rit loss compromises ROS-dependent mTORC2 …

Bovine Colostrum Supplementation Optimises Earnings, Performance And Recovery In Racing Thoroughbreds, C. K. Fenger, Thomas Tobin, P. J. Casey, Edward A. Roualdes, J. L. Langemeier, D. M. Haines

Maxwell H. Gluck Equine Research Center Faculty Publications

Bovine colostrum (BC) is the first milk produced by cows after calving and contains numerous beneficial substances for the immunity and development of the newborn calf. Because of the growth and immune factors in BC, it has become an attractive supplement for use by athletes to support immunity and health during athletic performance. In order to evaluate the effects of oral BC supplementation on equine athletes, this study evaluated the earnings, performance, recovery and incidence of upper respiratory infections (URTI) in racing horses. The study design was a randomized cross-over racing performance study. 21 horses in race training were randomly …

Cd151-Α3Β1 Integrin Complexes Suppress Ovarian Tumor Growth By Repressing Slug-Mediated Emt And Canonical Wnt Signaling, Lauren A. Baldwin, John T. Hoff, Jason Lefringhouse, Michael Zhang, Changhe Jia, Zeyi Liu, Sonia Erfani, Hongyan Jin, Mei Xu, Qing-Bai She, John R. Van Nagell Jr., Chi Wang, Li Chen, Rina Plattner, David M. Kaetzel, Jia Luo, Michael Lu, Dava West, Chunming Liu, Fred R. Ueland, Ronny Drapkin, Binhua P. Zhou, Xiuwei H. Yang

Pharmacology and Nutritional Sciences Faculty Publications

Human ovarian cancer is diagnosed in the late, metastatic stages but the underlying mechanisms remain poorly understood. We report a surprising functional link between CD151-α3β1 integrin complexes and the malignancy of serous-type ovarian cancer. Analyses of clinical specimens indicate that CD151 expression is significantly reduced or diminished in 90% of metastatic lesions, while it remains detectable in 58% of primary tumors. These observations suggest a putative tumor-suppressing role of CD151 in ovarian cancer. Indeed, our analyses show that knocking down CD151 or α3 integrin enhances tumor cell proliferation, growth and ascites production in nude mice. These changes are accompanied by …

Tall Fescue Seed Extraction And Partial Purification Of Ergot Alkaloids, Huihua Ji, Franklin Fannin, J. Klotz, Lowell P. Bush

Kentucky Tobacco Research and Development Center Faculty Publications

Many substances in the tall fescue/endophyte association (Schedonorus arundinaceus/Epichloë coenophiala) have biological activity. Of these compounds only the ergot alkaloids are known to have significant mammalian toxicity and the predominant ergot alkaloids are ergovaline and ergovalinine. Because synthetically produced ergovaline is difficult to obtain, we developed a seed extraction and partial purification protocol for ergovaline/ergovalinine that provided a biologically active product. Tall fescue seed was ground and packed into several different sized columns for liquid extraction. Smaller particle size and increased extraction time increased efficiency of extraction. Our largest column was a 114 × 52 × …

Mono- And Digalactosyldiacylglycerol Lipids Function Nonredundantly To Regulate Systemic Acquired Resistance In Plants, Qing-Ming Gao, Keshun Yu, Ye Xia, M. B. Shine, Caixia Wang, Duroy Navarre, Aardra Kachroo, Pradeep Kachroo

Plant Pathology Faculty Publications

The plant galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) have been linked to the anti-inflammatory and cancer benefits of a green leafy vegetable diet in humans due to their ability to regulate the levels of free radicals like nitric oxide (NO). Here, we show that DGDG contributes to plant NO as well as salicylic acid biosynthesis and is required for the induction of systemic acquired resistance (SAR). In contrast, MGDG regulates the biosynthesis of the SAR signals azelaic acid (AzA) and glycerol-3-phosphate (G3P) that function downstream of NO. Interestingly, DGDG is also required for AzA-induced SAR, but MGDG is not. Notably, …

Not Just A Theory--The Utility Of Mathematical Models In Evolutionary Biology, Maria R. Servedio, Yaniv Brandvain, Sumit Dhole, Courtney L. Fitzpatrick, Emma E. Goldberg, Caitlin A. Stern, Jeremy Van Cleve, D. Justin Yeh

Biology Faculty Publications

Progress in science often begins with verbal hypotheses meant to explain why certain biological phenomena exist. An important purpose of mathematical models in evolutionary research, as in many other fields, is to act as “proof-of-concept” tests of the logic in verbal explanations, paralleling the way in which empirical data are used to test hypotheses. Because not all subfields of biology use mathematics for this purpose, misunderstandings of the function of proof-of-concept modeling are common. In the hope of facilitating communication, we discuss the role of proof-of-concept modeling in evolutionary biology.

Forage News [2014-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentuckians at AFGC
• Forages at KCA
• Select Forage Varieties Wisely
• How Good is Your Hay?
• 35th Kentucky Alfalfa Conference
• Vegetative Identification of Small Grains
• 2014 UK Robinson Center Mountain Ag Week a Success
• Transgenic Reduced-Lignin Alfalfa Gains Deregulation

De La Reina-Madre De La Nación A La Tarasca: Para Un Análisis De Los Discursos De Juramentación Las Presidentas Electas Latinoamericanas, Yanira B. Paz

Hispanic Studies Faculty Publications

El propósito de este trabajo es estudiar desde la perspectiva del análisis crítico del discurso (ACD), los discursos de juramentación al cargo de las presidentas electas latinoamericanas. Se parte de la idea de que estos discursos constituyen la plataforma filosófica-política que, basada sobre las expectativas que llevaron al poder a estas líderes, constituirá el motor de acción del gobierno, así como también un criterio para valorar su implementación y efectividad. Desde la perspectiva de los estudios de género constituyen un proceso para la construcción de la identidad de la imagen de la líder.

This is a study from the perspective …