Digital Commons Network^™

An Evaluation Of The Quality Of Mens 100% Cotton Jersey Knit T-Shirts Representing Three Retail Categories, Jeanne Oakes Badgett

Theses and Dissertations--Retailing and Tourism Management

The purpose of this research was to evaluate the quality of design, materials, construction, appearance, and performance of mens 100% cotton jersey knit t-shirts from three retail categories: mass merchant, fast fashion, and better. These retail categories were represented by brands Fruit of the Loom, H&M, and Brooks Brothers, respectively. A convenience sample was comprised of 78 t-shirts. 13 white and 13 navy t-shirts from each brand were used for testing according to ASTM and AATCC standards and specifications. Evaluations and measurements were conducted before washing, and after one, five, ten, and twenty laundry cycles. The t-shirts were evaluated for …

Role Of Skeletal Paracrine Signals In The Proliferation And Chondrogenic Differentiation Of Interzone Cells, Parvathy Thampi

Theses and Dissertations--Veterinary Science

Articular cartilage in mammals has a limited intrinsic capacity to repair structural injuries and defects, a fact that contributes to the chronic and progressive nature of osteoarthritis. Current treatment modalities do not enable articular cartilage to achieve a complete and permanent restoration of normal structure and function with large or partial thickness lesions. In contrast to mammals, Mexican axolotl salamanders (Ambystoma mexicanum) have demonstrated the remarkable ability to spontaneously and completely repair even large joint cartilage lesions, an intrinsic healing process that involves interzone cells in the intraarticular space. Further, when interzone tissue is transplanted into critical sized …

Establishment Of American Chestnuts On Surface Mined Lands In Appalachian Coalfields Region, Michael E. French

Theses and Dissertations--Forestry and Natural Resources

American chestnut (Castanea dentata (Marsh.) Borkh.) was an dominant hardwood throughout eastern North America until the introduction of an exotic fungus (Cryphonectria parasitica (Murr.) Barr) in the early 20^th century devastated American chestnut populations. Chestnuts that have been bred for blight resistance have recently become available for testing. Due to the overlap of American chestnut’s natural range and the Appalachian coalfields region, the potential establishment of founder populations of blight-resistant chestnuts on reclaimed coal surface mines has recently gained attention. This work examined chestnut establishment on a surface mine in eastern Kentucky to assess factors controlling early …

Biology And Detection Of Pregnanes During Late Gestation In The Mare, Michelle Arelia Ann Wynn

Theses and Dissertations--Veterinary Science

Progesterone in the mare declines to almost undetectable concentrations in late gestation. It’s metabolized into several pregnanes, some circulating at very high concentrations. Although the function of many pregnanes remains unclear, 5α-dihydroprogesterone and allopregnanolone are bioactive. Measurements of pregnanes in late gestation are typically by immunoassay, although results are confounded by cross-reactivity with related pregnanes. Conversely, liquid chromatography tandem mass spectrometry (LC-MS/MS) allows differentiation of individual pregnanes. The purposes of these studies were: 1) to evaluate the ability of a 5α-reductase inhibitor, dutasteride, to alter pregnane metabolism and pregnancy outcome, 2) to evaluate changes in target pregnanes in late gestation …

Survival And Cause-Specific Mortality Of White-Tailed Deer (Odocoileus Virginianus) Neonates In A Southeastern Kentucky Population, Joseph R. Mcdermott

Theses and Dissertations--Forestry and Natural Resources

Maintaining desired numbers of wildlife species requires an understanding of species-specific population dynamics. For ungulate species such as the white-tailed deer (Odocoileus virginianus), understanding the neonatal survival rate of a population and factors that influence that survival rate, may be two of the most important factors to successful deer management. We examined neonatal survival in an eastern Kentucky population of deer living in relatively low densities (/ km²), with adequate habitat and supposedly poor population growth. Neonates (102) were captured in the summer birth periods of 2014 - 2016 and radio-monitored until the beginning of the …

An Investigation Into Specific Seminal Plasma Proteins And Their Effect On The Innate Immune Response To Breeding In The Mare, Carleigh Elizabeth Fedorka

Theses and Dissertations--Veterinary Science

The mare experiences a transient innate immune response to breeding, the resolution of which is crucial for optimal fertility. The majority of mares are able to modulate this inflammation in a timely fashion, but a subpopulation exists which fail to do so and are considered susceptible to persistent breeding-induced endometritis (PBIE). Seminal plasma has been shown to modulate aspects of this inflammation. Recently, two seminal plasma proteins have garnered interest for their immune modulating properties: cysteine-rich secretory protein-3 (CRISP-3) and lactoferrin. These proteins have been found to alter the binding between sperm and neutrophils based on sperm viability in vitro …

Analysis Of Humoral Immune Responses In Horses With Equine Protozoal Myeloencephalitis, Catherine-Jane Angwin

Theses and Dissertations--Veterinary Science

Equine protozoal myeloencephalitis (EPM), caused by the protozoan parasite Sarcocystis neurona, is one of the most important neurological diseases of horses in the Americas. While seroprevalence of S. neurona in horses is high, clinical manifestation of EPM occurs in less than 1% of infected horses. Factors governing the occurrence and severity of EPM are largely unknown, although horse immunity might play an important role in clinical outcome. We hypothesize that EPM occurs due to an aberrant immune response, which will be discernable in the equine IgG subisotypes a, b, and (T) that recognize S. neurona in infected diseased horses versus …

Managing Upland Oak Forests With Disturbance And The Implications For Non-Native Species Invasions, Devin E. Black

Theses and Dissertations--Forestry and Natural Resources

Natural and anthropogenic disturbances have influenced forest stand structure and affected species compositions within forests for millennia. Disturbances such as fire, severe weather events, and forest management practices may result in significantly reduced tree biomass. Thus, these disturbances may lower canopy closure and stem density in support of forest management goals, such as open oak woodland restoration, or promote an increase in species richness within the forest understory. However, these types of disturbance may also produce the unwanted consequence of opening pathways for the invasion of non-native species. Once established, these non-native species may threaten native plant communities and biodiversity. …

Inflammation And Insulin Dysregulation In The Horse, Sarah E. Elzinga

Theses and Dissertations--Veterinary Science

Insulin dysregulation (ID) in the horse is receiving increasing attention as a serious health concern, in particular due to its association with the potentially career or life ending condition, laminitis. The role of inflammation and the immune system in ID as well as its associated health concerns has also been raised. However, the involvement of inflammation in and mechanisms behind ID in the horse remain unclear. Therefore, our overall hypothesis was that due in part to changes in their gut microbiota and plasma lipidome, horses with ID have changes in circulating proinflammatory markers, in particular in response to glycemic challenge, …

Lymphocyte-Mediated Inflamm-Aging In The Horse, Melissa Hope Siard

Theses and Dissertations--Veterinary Science

Senior horses (≥20 years) exhibit inflamm-aging, or chronic, low-grade inflammation that occurs systemically with aging, similarly to humans. Inflamm-aging has previously been characterized in the horse in circulation as well as specifically being mediated by lymphocytes and monocytes. In humans, inflamm-aging has been associated with increased morbidity and mortality. However, in the horse, relatively little about inflamm-aging is known regarding clinical effects or factors influencing severity. The contribution of lymphocytes to inflamm-aging of senior horses was examined, specifically through determining the relationships of inflamm-aging with various other health parameters, effects of seasonality, and the extent to which inflamm-aging can be …

Physiological Changes Associated With Pregnant Or Nonpregnant Mares Grazing Pastures Of Orchardgrass-Bluegrass, Kentucky 31 Tall Fescue Infected With Epichloë Coenophiala, Or Kyfa9821 Tall Fescue Infected With The Novel Endophyte Ar584, Victoria A. Taylor

Theses and Dissertations--Veterinary Science

Kentucky 31 tall fescue (KY31) infected with the common toxic endophyte strains of Epichloё coenophiala produces toxic alkaloids that improve plant vigor, but cause numerous adverse effects in grazing animals. Researchers developed a variety of KY31 containing an alternative strain of E. coenophiala, termed novel endophyte (NE). Adverse health effects in mares have not been evaluated.

Experiments in this thesis tested the hypothesis that the NE pasture does not cause adverse effects typically associated with KY31. Specific aims were to: 1) compare forage ergovaline concentrations between KY31 vs NE pastures; 2) evaluate palmar artery diameters in mares grazing KY31, …

Altitude- And Sex-Specific Variation In Roosting Ecology And Thermoregulation Of Myotis Lucifugus In Yellowstone National Park, Alexandra C. Slusher

Theses and Dissertations--Forestry and Natural Resources

Fifty-nine female and six male little brown myotis (Myotis lucifugus) were radio-tagged during the summers of 2012, 2013, 2014, and 2015 in Yellowstone National Park. The grand models for daily maximum skin temperature (F_98,154 = 1.55, P = 0.007), daily minimum skin temperature (F_98,154 = 1.33, P = 0.05), and daily variation in skin temperature (F_98,154 = 1.56, P = 0.006) were significant across roost type and reproductive condition class for adult females. Roosts were classified into Types A (warmest roosts), B (roosts with largest daily temperature variance), and C (stable and cool roosts) depending …

Establishment Of Serological Reference Ranges For Elk In Kentucky And Effects Of Quarantine And Translocation On Elk, Aaron M. Hildreth

Theses and Dissertations--Forestry and Natural Resources

Establishing blood serum profiles is important for understanding animal disease ecology and nutrition, the effects of capture and immobilization, and general physiological variation among individuals and populations. Elk (Cervus elaphus nelsoni) have been successfully translocated to several states for reintroduction or population augmentation, including most recently in Kentucky, where over the course of three years (2011-13) wild elk were captured, temporarily quarantined, and subsequently moved to Missouri and Virginia to establish populations in those states. I used this opportunity to collect a variety of biological samples, including blood from which I present and compare serological profiles for a …

Evolution Of Equine Arteritis Virus During Persistent Infection In The Reproductive Tract Of The Stallion And The Male Donkey, Bora Nam

Theses and Dissertations--Veterinary Science

Equine arteritis virus (EAV) establishes persistent infection in the stallion reproductive tract, and the carrier stallion continues to shed virus in semen for weeks to years or lifelong. The objective of this study was to elucidate the intra-host evolution of EAV during persistent infection in stallions. Seven EAV seronegative stallions were experimentally infected with EAV KY84 strain and followed for 726 days post-infection, and sequential clinical samples including semen were collected for virus isolation and next-generation sequencing (NGS). In addition, archived sequential semen samples from two stallions that were naturally infected with EAV KY84 for a long-period (up to 10 …

Survival And Cause-Specific Mortality Of A Southeastern Kentucky Deer Population, Caleb Anderson Haymes

Theses and Dissertations--Forestry and Natural Resources

White-tailed deer are one of the most sought after game species in Kentucky. While much of the Commonwealth boasts high deer populations, those in southeast Kentucky are viewed as relatively low compared to other regions, even after a decade of restrictive doe harvest and multiple years of population supplementation via translocation. We studied survival and cause specific mortality of a local population of deer near the Redbird District of the Daniel Boone National Forest in Clay and Leslie County, Kentucky from January 2014 - January 2017. We estimated female annual survival at 0.89 (CI: 0.88-0.87), with an overall 3-year survival …

Exploring Predator-Prey Interactions In Agroecosystems Through Molecular Gut-Content Analysis, Kacie J. Athey

Theses and Dissertations--Entomology

Generalist predators can contribute to vital ecosystem services by potentially inducing trophic cascades as natural enemies of pests in agroecosystems. As the human population of the world gets larger, we need to produce more food on ever-smaller swaths of available land relying on ecosystem services, in the form of pest control, that may contribute to agricultural sustainability. Teasing apart the exact trophic linkages between predators and prey is a vital first step and essential to uncovering which predators are inducing trophic cascades and should be enhanced through conservation biological control.

Combined with ecological experimentation, the main tool used throughout my …

Evaluation Of Pyriproxyfen Applied In Barrier Sprays For Mosquito Suppression, Andrea Glenn Skiles

Theses and Dissertations--Entomology

Despite advances in mosquito management, mosquito-borne disease in the United States is still of relevant public health concern and vector control is a top priority in preventing transmission of pathogens. Insecticide barrier sprays have become a common tool for suppression of mosquitoes in single-homeowner backyards. The application of the synthetic pyrethroid, lambda-cyhalothrin to perimeter vegetation with a backpack sprayer has been shown to significantly suppress mosquito levels for around 6 weeks. In an attempt to lengthen the effective duration of treatment, the IGR, pyriproxyfen, was added to a backpack mist blower with lambda-cyhalothrin, as adult mosquitoes exposed to pyriproxyfen have …

Formative Evaluation Of A Family Cooperation Board Game, Joseph Hannan

Theses and Dissertations--Family Sciences

A lack of credible evidence demonstrating the effectiveness of play therapy and the use of therapeutic board game in play therapy exists (Phillips, 2010; Matorin, 1996). Parent involvement is a key variable in the effectiveness of play therapy (Kottman, Stother, and Deniger, 2001). Formative research was used in this study to evaluate of The Super Family Board Game™ (SFBG) in order to develop an effective therapeutic board game aimed at enhancing family cooperation and cohesion. As the first formative evaluation of a therapeutic board game, this study provides future research implications for developing and testing therapeutic board games.

Binding, Protection, And Rna Delivery Properties Of Porous Silica Nanoparticles In Spodoptera Frugiperda Cells, Emily Nadeau

Theses and Dissertations--Entomology

Traditional methods of pest control are threatened by the development of insecticide resistance, both to traditional insecticides and Bt toxins. Discovery of RNA interference (RNAi) has created opportunities to develop new insect control mechanisms. However, RNAi responses appear to be robust in coleopteran pests, but other orders, e.g. Lepidoptera and Hemiptera, present varied or ineffective RNAi responses. Current delivery strategies for double-stranded RNA (dsRNA) include microinjection, ingestion, and soaking. These approaches have benefits and problems. This study investigates the potential for porous silica nanoparticles (pSNPs) to improve the delivery of dsRNA and induce an RNAi response in Spodoptera frugiperda cells. …

The Effects Of A 16-Week Introductory Nutrition Course On Dietary Habits And Body Composition Of College Students, Emily Ashton

Theses and Dissertations--Nutrition and Food Systems

As the rates of obesity continue to increase among adolescents and young adults, adopting healthy dietary and lifestyle habits is necessary in order to prevent obesity-related chronic disease later in life. Although several studies have addressed nutrition education and its effect on weight in college students, few studies have assessed percent fat as it relates to a semester-long nutrition course. As such, the effectiveness of a formal introductory nutrition course on lifestyle habits and percent fat of college students was examined. The current study aimed to address the relationship between percent body fat and nutrition education over the course of …

Identification Of Novel Sleep Related Genes From Large Scale Phenotyping Experiments In Mice, Shreyas Joshi

Theses and Dissertations--Biology

Humans spend a third of their lives sleeping but very little is known about the physiological and genetic mechanisms controlling sleep. Increased data from sleep phenotyping studies in mouse and other species, genetic crosses, and gene expression databases can all help improve our understanding of the process. Here, we present analysis of our own sleep data from the large-scale phenotyping program at The Jackson Laboratory (JAX), to identify the best gene candidates and phenotype predictors for influencing sleep traits.

The original knockout mouse project (KOMP) was a worldwide collaborative effort to produce embryonic stem (ES) cell lines with one of …

Evaluating Nonstructural Carbohydrate Variation Of Cool-Season Grasses Based On Genotype, Management And Environment, Kelly Joan Prince

Theses and Dissertations--Plant and Soil Sciences

Understanding how nonstructural carbohydrates fluctuate in pastures and being able to quantify them is an essential component in successfully managing grazing animals that may require high or low nonstructural carbohydrate diets. The objectives of this study were 1) to evaluate the effects of genotype, management, and environment on water-soluble carbohydrates (WSC) and ethanol-soluble carbohydrates (ESC) in cool-season grass pastures in central Kentucky, and 2) to develop near-infrared reflectance spectroscopy (NIRS) equations to predict WSC and ESC in cool-season grasses. Ten cool-season grass cultivars consisting of Kentucky bluegrass, tall fescue, orchardgrass and perennial ryegrass were sampled in the morning and afternoon …

The Role Of Alternative Polyadenylation Mediated By Cpsf30 In Arabidopsis Thaliana, Guijie Hao

Theses and Dissertations--Plant and Soil Sciences

Drought stress is considered one of the most devastating abiotic stress factors that limit crop productivity for modern agriculture worldwide. There is a large range of physiological and biochemical responses induced by drought stress. The responses range from physiological and biochemical to regulation at transcription and posttranscriptional levels. Post-transcription, the products encoded by eukaryotic genes must undergo a series of modifications to become a mature mRNA. Polyadenylation is an important one in terms of regulation. Polyadenylation impacts gene expression through determining the coding and regulation potential of the mRNA, especially when different mRNAs from the same gene may be polyadenylated …

The Effects Of Depletion And Brain Stimulation On Motivation, Sarah Beth Bell

Theses and Dissertations--Psychology

Mental fatigue decreases motivation. I tested whether applying electricity to a self-control region of the brain would replenish some of the motivation normally lost during mental fatigue. 224 people participated in this study. Each person received real or placebo brain stimulation while undergoing activities that increased mental fatigue. The dependent variable was a task where participants had to perform work by clicking a computer mouse repeatedly. Before performing this task, participants indicated how hard they were planning to work on this motivation task. Participants who received real brain stimulation were able to perform more work, but only if they also …

Why We Sing Along: Measurable Traits Of Successful Congregational Songs, Daniel Read

Theses and Dissertations--Music

Songwriters have been creating music for the church for hundreds of years. The songs have gone through many stylistic changes from generation to generation, yet, each song has generated congregational participation. What measurable, traceable qualities of congregational songs exist from one generation to the next?

This document explores the history and development of Congregational Christian Song (CCS), to discover and document the similarities between seemingly contrasting styles of music. The songs analyzed in this study were chosen because of their wide popularity and broad dissemination among non-denominational churches in the United States. While not an exhaustive study, this paper reviews …

Novel Evidence That The Mannan-Binding Lectin Pathway Of Complement Activation Plays A Pivotal Role In Triggering Mobilization Of Hematopoietic Stem/Progenitor Cells By Activation Of Both The Complement And Coagulation Cascades, M. Adamiak, A. Abdelbaset-Ismail, M. Suszynska, Ahmed K. Abdel-Latif, J. Ratajczak, M. Z. Ratajczak

Internal Medicine Faculty Publications

No abstract provided.

Ligand Trap Of The Activin Receptor Type Iia Inhibits Osteoclast Stimulation Of Bone Remodeling In Diabetic Mice With Chronic Kidney Disease, Toshifumi Sugatani, Olga A. Agapova, Yifu Fang, Alycia G. Berman, Joseph M. Wallace, Hartmut H. Malluche, Marie-Claude Faugere, William Smith, Victoria Sung, Keith A. Hruska

Internal Medicine Faculty Publications

Dysregulation of skeletal remodeling is a component of renal osteodystrophy. Previously, we showed that activin receptor signaling is differentially affected in various tissues in chronic kidney disease (CKD). We tested whether a ligand trap for the activin receptor type 2A (RAP-011) is an effective treatment of the osteodystrophy of the CKD-mineral bone disorder. With a 70% reduction in the glomerular filtration rate, CKD was induced at 14 weeks of age in the ldlr−/− high fat-fed mouse model of atherosclerotic vascular calcification and diabetes. Twenty mice with CKD, hyperphosphatemia, hyperparathyroidism, and elevated activin A were treated with RAP-011, wherease 19 …

From Dose To Response: In Vivo Nanoparticle Processing And Potential Toxicity, Uschi M. Graham, Gary Jacobs, Robert A. Yokel, Burtron H. Davis, Alan K. Dozier, M. Eileen Birch, Michael T. Tseng, Günter Oberdörster, Alison Elder, Lisa Delouise

Pharmaceutical Sciences Faculty Publications

Adverse human health impacts due to occupational and environmental exposures to manufactured nanoparticles are of concern and pose a potential threat to the continued industrial use and integration of nanomaterials into commercial products. This chapter addresses the inter-relationship between dose and response and will elucidate on how the dynamic chemical and physical transformation and breakdown of the nanoparticles at the cellular and subcellular levels can lead to the in vivo formation of new reaction products. The dose-response relationship is complicated by the continuous physicochemical transformations in the nanoparticles induced by the dynamics of the biological system, where dose, bio-processing, and …

Processing Of Spatial Information In Social And Non-Social Stimuli By Opioid-Exposed And Non-Exposed Newborns, Alyson J. Hock

Theses and Dissertations--Psychology

The ability to process information from faces is important for effective social functioning. Adults are experts at this function. It has been suggested that the encoding of configural spatial relations among facial features (e.g., the distance between the eyes) contributes to this expertise. I investigated the developmental origin of face processing expertise by studying typically developing newborns’ sensitivity to the distance between the eyes and between the nose and the mouth in face stimuli. Further, I investigated whether prenatal opioid exposure is associated with neonates’ processing of spatial information in social and non-social stimuli. Infants with prenatal opioid-exposure are at …

Modeling Faceted Browsing With Category Theory For Reuse And Interoperability, Daniel R. Harris

Theses and Dissertations--Computer Science

Faceted browsing (also called faceted search or faceted navigation) is an exploratory search model where facets assist in the interactive navigation of search results. Facets are attributes that have been assigned to describe resources being explored; a faceted taxonomy is a collection of facets provided by the interface and is often organized as sets, hierarchies, or graphs. Faceted browsing has become ubiquitous with modern digital libraries and online search engines, yet the process is still difficult to abstractly model in a manner that supports the development of interoperable and reusable interfaces. We propose category theory as a theoretical foundation for …