Digital Commons Network^™

Whey Protein Supplementation Post Resistance Exercise In Elderly Men Induces Changes In Muscle Mirna's Compared To Resistance Exercise Alone, Randall F. D'Souza, Nina Zeng, James F. Markworth, Vandre C. Figueiredo, Christopher P. Hedges, Aaron C Petersen, Paul A Della Gatta, David Cameron-Smith, Cameron J. Mitchell

Center for Muscle Biology Faculty Publications

Progressive muscle loss with aging results in decreased physical function, frailty, and impaired metabolic health. Deficits in anabolic signaling contribute to an impaired ability for aged skeletal muscle to adapt in response to exercise and protein feeding. One potential contributing mechanism could be exerted by dysregulation of microRNAs (miRNAs). Therefore, the aim of this study was to determine if graded protein doses consumed after resistance exercise altered muscle miRNA expression in elderly men. Twenty-three senior men (67.9 ± 0.9 years) performed a bout of resistance exercise and were randomized to consume either a placebo, 20 or 40 g of whey …

Caloric Restriction Alters Postprandial Responses Of Essential Brain Metabolites In Young Adult Mice, Lucille M. Yanckello, Lyndsay E. A. Young, Jared D. Hoffman, Robert P. Mohney, Mignon A. Keaton, Erin L. Abner, Ai-Ling Lin

Sanders-Brown Center on Aging Faculty Publications

Caloric restriction (CR) has been shown to extend longevity and protect brain function in aging. However, the effects of CR in young adult mice remain largely unexplored. In addition to the fundamental, long-term changes, recent studies demonstrate that CR has a significant impact on transient, postprandial metabolic flexibility and turnover compared to control groups. The goal of this study was to identify the brain metabolic changes at a transient (2 h) and steady (6 h) postprandial state in young mice (5–6 months of age) fed with CR or ad libitum (AL; free eating). Using metabolomics profiling, we show that CR …

Downregulation Of Orco And 5-Htt Alters Nestmate Discrimination In The Subterranean Termite Ondontotermes Formosanus (Shiraki), Pengdong Sun, Shuxin Yu, Austin Merchant, Chaoliang Lei, Xuguo Zhou, Qiuying Huang

Entomology Faculty Publications

Nestmate discrimination allows social insects to recognize nestmates from non-nestmates using colony-specific chemosensory cues, which typically evoke aggressive behavior toward non-nestmates. Functional analysis of genes associated with nestmate discrimination has been primarily focused on inter-colonial discrimination in Hymenopterans, and parallel studies in termites, however, are grossly lacking. To fill this gap, we investigated the role of two genes, Orco and 5-HTT, associated with chemosensation and neurotransmission respectively, in nestmate discrimination in a highly eusocial subterranean termite, Odontotermes formosanus (Shiraki). We hypothesized that knocking down of these genes will compromise the nestmate recognition and lead to the antagonistic behavior. To …

Geographic Biases In Bee Research Limits Understanding Of Species Distribution And Response To Anthropogenic Disturbance, Mary A. Jamieson, Adrian L. Carper, Caleb J. Wilson, Virginia L. Scott, Jason Gibbs

Entomology Faculty Publications

Habitat loss and degradation due to agricultural intensification and urbanization are key threats facing wild pollinators, especially bees. However, data on the distribution and abundance of most of the world's 20,000+ bee species is lacking, making it difficult to assess the effects of anthropogenic disturbance through time. Moreover, there are geographic biases in the study of bees creating gaps in our understanding of species distributions and regional patterns of diversity. Research efforts are often focused around cities or field stations associated with universities and other research institutions. In this perspectives paper, we provide examples of geographic bias in knowledge regarding …

The Benefits Of Lasix For Exercise-Induced Pulmonary Hemorrhage: 50 Years Of Science And Clinical Experience, Kimberly Brewer, Clara Fenger, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

No abstract provided.

Forage News [2019-06], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Managing Pastures this Summer
• 2019 Western Kentucky Summer Forage Tour: Resuscitating a Rundown Farm
• The Wonder Grass: The Story of Tall Fescue in the United States now available
• UK Weed Sceicne to Host a Pair of Field Days at UKREC in Princeton
• Grass Decline? Check Your Cutting Height
• We're Producing and Feeding Less Hay

Amplicon Sequencing Of 42 Nuclear Loci Supports Directional Gene Flow Between South Pacific Populations Of A Hydrothermal Vent Limpet, Sophie Plouviez, Abigail Leavitt Labella, David W. Weisrock, F. A. Bastiaan Von Meijenfeldt, Bernard Ball, Joseph E. Neigel, Cindy L. Van Dover

Biology Faculty Publications

In the past few decades, population genetics and phylogeographic studies have improved our knowledge of connectivity and population demography in marine environments. Studies of deep‐sea hydrothermal vent populations have identified barriers to gene flow, hybrid zones, and demographic events, such as historical population expansions and contractions. These deep‐sea studies, however, used few loci, which limit the amount of information they provided for coalescent analysis and thus our ability to confidently test complex population dynamics scenarios.

In this study, we investigated population structure, demographic history, and gene flow directionality among four Western Pacific hydrothermal vent populations of the vent limpet Lepetodrilus …

Limbic-Predominant Age-Related Tdp-43 Encephalopathy (Late): Consensus Working Group Report, Peter T. Nelson, Dennis W. Dickson, John Q. Trojanowski, Clifford R. Jack, Patricia A. Boyle, Konstantinos Arfanakis, Rosa Rademakers, Irina Alafuzoff, Johannes Attems, Carol Brayne, Ian T. S. Coyle-Gilchrist, Helena C. Chui, David W. Fardo, Margaret E. Flanagan, Glenda Halliday, Suvi R. K. Hokkanen, Sally Hunter, Gregory A. Jicha, Yuriko Katsumata, Claudia H. Kawas, C. Dirk Keene, Gabor G. Kovacs, Walter A. Kukull, Allan I. Levey, Nazanin Makkinejad, Thomas J. Montine, Shigeo Murayama, Melissa E. Murray, Sukriti Nag, Robert A. Rissman, William W. Seeley, Reisa A. Sperling, Charles L. White Iii, Lei Yu, Julie A. Schneider

Sanders-Brown Center on Aging Faculty Publications

We describe a recently recognized disease entity, limbic-predominant age-related TDP-43 encephalopathy (LATE). LATE neuropathological change (LATE-NC) is defined by a stereotypical TDP-43 proteinopathy in older adults, with or without coexisting hippocampal sclerosis pathology. LATE-NC is a common TDP-43 proteinopathy, associated with an amnestic dementia syndrome that mimicked Alzheimer’s-type dementia in retrospective autopsy studies. LATE is distinguished from frontotemporal lobar degeneration with TDP-43 pathology based on its epidemiology (LATE generally affects older subjects), and relatively restricted neuroanatomical distribution of TDP-43 proteinopathy. In community-based autopsy cohorts, ∼25% of brains had sufficient burden of LATE-NC to be associated with discernible cognitive impairment. Many …

Validation Of A Commercial Automated Body Condition Scoring System On A Commercial Dairy Farm, Israel L. Mullins, Carissa M. Truman, Magnus R. Campler, Jeffrey M. Bewley, Joao H. C. Costa

Animal and Food Sciences Faculty Publications

Body condition scoring (BCS) is the management practice of assessing body reserves of individual animals by visual or tactile estimation of subcutaneous fat and muscle. Both high and low BCS can negatively impact milk production, disease, and reproduction. Visual or tactile estimation of subcutaneous fat reserves in dairy cattle relies on their body shape or thickness of fat layers and muscle on key areas of the body. Although manual BCS has proven beneficial, consistent qualitative scoring can be difficult to implement. The desirable BCS range for dairy cows varies within lactation and should be monitored at multiple time points throughout …

Stimulatory Effect Of 5-Hydroxytryptamine (5-Ht) On Rat Capsaicin-Sensitive Lung Vagal Sensory Neurons Via Activation Of 5-Ht3 Receptors, Chun-Chun Hsu, Ting Ruan, Lu-Yuan Lee, You Shuei Lin

Physiology Faculty Publications

5-hydroxytryptamine (5-HT) is an inflammatory mediator known to be released in lung. Capsaicin-sensitive lung vagal (CSLV) afferents function as a primary sensor for detecting chemical stimuli and produce consequent reflexes during lung inflammation. To characterize the effect of 5-HT on CSLV afferents, responses of cardiorespiratory reflexes and single-unit C-fiber afferents to right-atrial injections of 5-HT were investigated in anesthetized Sprague-Dawley rats. Bolus injection of 5-HT (8 μg/kg) caused an immediate augmented breath and apnea, accompanied by hypotension and bradycardia. These initial responses were then followed by a brief pressor response and a more sustained depressor response. After a perineural treatment …

Rediscovery Of The Endangered Carchi Andean Toad, Rhaebo Colomai (Hoogmoed, 1985), In Ecuador, With Comments On Its Conservation Status And Extinction Risk, Carolina Reyes-Puig, Gabriela B. Bittencourt-Silva, María Torres-Sánchez, Mark Wilkinson, Jeffrey W. Streicher, Simon T. Maddock, Ramachandran Kotharambath, Hendrik Müller, Francesca Nicole Angiolani Larrera, Diego Amieda-Reinoso, Santiago R. Ron, Diego Francisco Cisneros-Heredia

Neuroscience Faculty Publications

Since 1984 there have been no records of Rhaebo colomai (Hoogmoed, 1985) within the territory of Ecuador. This species was known from 2 localities in the province of Carchi, northwestern Ecuador, and the department of Nariño, southwestern Colombia, which were reported in 1979 and 2015, respectively. We report the recent sightings of R. colomai at 3 new localities in Ecuador and discuss and evaluate this species’ extinction risk and conservation status.

A High-Throughput Screen Indicates Gemcitabine And Jak Inhibitors May Be Useful For Treating Pediatric Aml, Christina D. Drenberg, Anang Shelat, Jinjun Dang, Anitria Cotton, Shelley J. Orwick, Mengyu Li, Jae Yoon Jeon, Qiang Fu, Daelynn R. Buelow, Marissa Pioso, Shuiying Hu, Hiroto Inaba, Raul C. Ribeiro, Jeffrey E. Rubnitz, Tanja A. Gruber, R. Kiplin Guy, Sharyn D. Baker

Pharmaceutical Sciences Faculty Publications

Improvement in survival has been achieved for children and adolescents with AML but is largely attributed to enhanced supportive care as opposed to the development of better treatment regimens. High risk subtypes continue to have poor outcomes with event free survival rates < 40% despite the use of high intensity chemotherapy in combination with hematopoietic stem cell transplant. Here we combine high-throughput screening, intracellular accumulation assays, and in vivo efficacy studies to identify therapeutic strategies for pediatric AML. We report therapeutics not currently used to treat AML, gemcitabine and cabazitaxel, have broad anti-leukemic activity across subtypes and are more effective relative to the AML standard of care, cytarabine, both in vitro and in vivo. JAK inhibitors are selective for acute megakaryoblastic leukemia and significantly prolong survival in multiple preclinical models. Our approach provides advances in the development of treatment strategies for pediatric AML.

Secreted Tumor-Associated Cytochrome As A Blood-Based Biomarker For Cancer, Rolf Joseph Craven

Pharmacology and Nutritional Sciences Faculty Patents

Disclosed herein are methods for detecting Pgrmc 1 in bodily fluids, e.g., blood, plasma and serum, wherein detection of Pgrmcl is a biomarker for the presence of cancer, e.g., lung cancer or head or neck cancer. Pgrmc 1 levels in bodily fluids may be used to predict patient prognosis, e.g., survival and response to therapy.

Little Fish In A Big Pond : A Kentucky Public Library Joins Naco, Victoria Greenwell, Kristin Anderson, Robert Collins

OVGTSL 2019

Many public libraries undervalue the need for good authority control and since NACO has vastly more academic libraries than public libraries, many of our authority needs may be going unanswered. Our library catalog alone has tens of thousands of local authorities that need to be created. How many does yours have?

This presentation will cover the reasons this library chose to join NACO, as well as its long and error-filled path to get its application approved, a story that is humorous in its coincidence, misunderstandings and just sheer, dumb luck. We will also touch on the extensive training involved and …

Opening Keynote: Storytelling & Power In Technical Services, Hannah Buckland

OVGTSL 2019

Stories—as well as language and words themselves—articulate value, identifying what is significant about our histories and our relationships. In this way, storytelling is a form of power, a way of organizing the world. Technical services work, in turn, does more than create, locate, or describe; it also contributes to an ongoing story libraries communicate to users, a story about how something came to be, a story about power dynamics, and a story of how something is connected to everything.

Deletion Of P38Α Mapk In Microglia Blunts Trauma-Induced Inflammatory Responses In Mice, Josh M. Morganti, Danielle S. Goulding, Linda J. Van Eldik

Sanders-Brown Center on Aging Faculty Publications

Traumatic brain injury (TBI) is a significant cause of morbidity and mortality in the USA and other developed countries worldwide. Following the initial mechanical insult, the brain’s primary innate immune effector, microglia, initiate inflammatory signaling cascades and pathophysiological responses that can lead to chronic neuroinflammation and neurodegenerative sequelae. The p38α MAPK signaling pathway in microglia is a key contributor to inflammatory responses to diverse disease-relevant stressors and injury conditions. Therefore, we tested here whether microglia p38α contributes to acute and persistent inflammatory responses induced by a focal TBI. We generated conditional cell-specific knockout of p38α in microglia using a CX3CR1 …

Water Permeability/Impermeability In Seeds Of 15 Species Of Caragana (Fabaceae), Dali Chen, Rui Zhang, Carol C. Baskin, Xiaowen Hu

Biology Faculty Publications

Majority legumes in the temperate and arctic zones have water-impermeable seeds (physical dormancy, PY). However, various authors have reported that seeds of some Caragana species are water-permeable and thus non-dormant. We (1) tested seeds of 15 species of Caragana matured in the same site in 2014, 2016 and/or 2017 for presence of PY, (2) determined if dry storage decreased or increased the percentage of seeds with PY and (3) located the site on the seed coat of 11 species where water enters the seed. Sixty-three percent and 45% of the seeds of C. roborovskyi had PY in 2016 and 2017, …

What Lies Beneath? Molecular Evolution During The Radiation Of Caecilian Amphibians, María Torres-Sánchez, David J. Gower, David Alvarez-Ponce, Christopher J. Creevey, Mark Wilkinson, Diego San Mauro

Neuroscience Faculty Publications

Background: Evolution leaves an imprint in species through genetic change. At the molecular level, evolutionary changes can be explored by studying ratios of nucleotide substitutions. The interplay among molecular evolution, derived phenotypes, and ecological ranges can provide insights into adaptive radiations. Caecilians (order Gymnophiona), probably the least known of the major lineages of vertebrates, are limbless tropical amphibians, with adults of most species burrowing in soils (fossoriality). This enigmatic order of amphibians are very distinct phenotypically from other extant amphibians and likely from the ancestor of Lissamphibia, but little to nothing is known about the molecular changes underpinning their radiation. …

The Relationship Between Psychological States And Health Perception In Individuals At Risk For Cardiovascular Disease, Kyoung Suk Lee, Frances J. Feltner, Alison L. Bailey, Terry A. Lennie, Misook L. Chung, Brittany L. Smalls, Donna L. Schuman, Debra K. Moser

Family and Community Medicine Faculty Publications

Backgrounds: Perceptions of health are important to motivate people to change behaviors. Non-adherence to healthy behaviors that prevent cardiovascular disease may result from inadequate health perceptions. However, there are few studies investigating relationships between health perceptions and psychological states.

Objective: To determine whether psychological states (ie, depressive symptoms and anxiety) are associated with the congruency between health perception and estimated risk for cardiovascular disease in adults with 2 or more cardiovascular disease risk factors.

Methods: Community dwellers at risk for cardiovascular disease were asked to complete the Patient Health Questionnaire-9 and the anxiety subscale of the Brief Symptom Inventory to …

Measurement Of Bystander Actions In Violence Intervention Evaluation: Opportunities And Challenges, Heather M. Bush, Samuel C. Bell, Ann L. Coker

Biostatistics Faculty Publications

Purpose of Review

This review discusses design and methodological challenges specific to measuring bystander actions in the evaluation of bystander-based violence prevention programming. “Bystanders” are defined as people who are present immediately before, during and/or after a violent event, but are not a perpetrator nor the intended victim. Bystander-based violence prevention programs seek to prevent or mitigate violent events by empowering bystanders to intervene on acts of violence and social norms that promulgate violence.

Recent Findings

Effective bystander-based violence prevention programs demonstrate increased bystander intentions, actions, and attitudes [Bringing in the Bystander: Banyard et al. J Community Psychol. 2007;35:463-481; iSCREAM: …

One Amino Acid Change Of Angiotensin Ii Diminishes Its Effects On Abdominal Aortic Aneurysm, Ya Wang, Yinchuan Xu, Congqing Wu, Hongguang Xia, Yingchao Wang, Jinliang Nan, Jinghai Chen, Hong Yu, Wei Zhu, Peng Shi, Alan Daugherty, Hong S. Lu, Jian'an Wang

Saha Cardiovascular Research Center Faculty Publications

Angiotensin (Ang) A is formed by the decarboxylation of the N terminal residue of AngII. The present study determined whether this one amino acid change impacted effects of AngII on abdominal aortic aneurysm (AAA) formation in mice. Computational analyses implicated that AngA had comparable binding affinity to both AngII type 1 and 2 receptors as AngII. To compare effects of these two octapeptides in vivo, male low-density lipoprotein receptor (Ldlr) or apolipoprotein E (Apoe) deficient mice were infused with either AngII or AngA (1 μg/kg/min) for 4 weeks. While AngII infusion induced AAA consistently in …

Predicting Habitat Choice After Rapid Environmental Change, Philip H. Crowley, Pete C. Trimmer, Orr Spiegel, Sean M. Ehlman, William S. Cuello, Andrew Sih

Biology Faculty Publications

Decisions made while searching for settlement sites (e.g., nesting, oviposition) often have major fitness implications. Despite numerous case studies, we lack theory to explain why some species are thriving while others are making poor habitat choices after environmental change. We develop a model to predict (1) which kinds of environmental change have larger, negative effects on fitness, (2) how evolutionary history affects susceptibility to environmental change, and (3) how much lost fitness can be recovered via readjustment after environmental change. We model the common scenario where animals search an otherwise inhospitable matrix, encountering habitats of varying quality and settling when …

Forage News [2019-05], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Register for 2019 Kentucky Fencing School in Russellville on May 30th
• Things in the Hay Production Book We Missed
• Insure High Quality Forage: Make Baleage
• Optimizing Production from Perennial Cool-Season Hayfields
• UK Equine Farm and Facilities Expo - May 30
• Purdue Extension Hosts Educational Events This Summer - June 21 and 22
• Tariffs, Water Reshuffled the Hay Export Deck

Associations Of First Trimester Co-Use Of Tobacco And Cannabis With Prenatal Immune Response And Psychosocial Well-Being, Kristin Ashford, Amanda Fallin-Bennett, Andrea Mccubbin, Amanda T. Wiggins, Sheila Barnhart, Joshua A. Lile

Perinatal Research and Wellness Center Faculty Publications

PURPOSE: This study aims to describe the association of first trimester co-use of tobacco and cannabis with maternal immune response and psychosocial well-being, relative to tobacco use only.

METHODS: A preliminary midpoint analysis included 138 pregnant women with biologically verified tobacco use, 38 of whom (28%) also tested positive for recent cannabis use. Maternal perceived stress (Perceived Stress Scale), depressive symptoms (Edinburgh Postnatal Depression Scale), and serum immune markers (IL-1β, IL-2, IL-6, IL-8, IL-10, TNFα, CRP, MMP8), were collected, although cytokine data were only available for 122 women.

RESULTS: Participant average age was 29.1 years, approximately half had a high …

Systematic Review Of Gastrointestinal Nematodes Of Horses From Australia, Muhammad A. Saeed, Ian Beveridge, Ghazanfar Abbas, Anne Beasley, Jenni Bauquier, Edwina Wilkes, Caroline Jacobson, Kris J. Hughes, Charles El-Hage, Ryan O'Handley, John Hurley, Lucy Cudmore, Peter Carrigan, Lisa Walter, Brett Tennent-Brown, Martin K. Nielsen, Abdul Jabbar

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: Equine gastrointestinal nematodes (GINs) have been the subject of intermittent studies in Australia over the past few decades. However, comprehensive information on the epidemiology of equine GINs, the efficacy of available anthelmintic drugs and the prevalence of anthelmintic resistance (AR) in Australasia is lacking. Herein, we have systematically reviewed existing knowledge on the horse GINs recorded in Australia, and main aspects of their pathogeneses, epidemiology, diagnoses, treatment and control.

Methods: Six electronic databases were searched for publications on GINs of Australian horses that met our inclusion criteria for the systematic review. Subsets of publications were subjected to review epidemiology, …

Development And Evaluation Of A One-Step Multiplex Real-Time Taqman® Rt-Qpcr Assay For The Detection And Genotyping Of Equine G3 And G14 Rotaviruses In Fecal Samples, Mariano Carossino, Maria E. Barrandeguy, Erdal Erol, Yanqiu Li, Udeni B. R. Balasuriya

Veterinary Science Faculty Publications

Background: Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and has a negative impact on equine breeding enterprises worldwide. Among ERVA strains infecting foals, the genotypes G3P[12] and G14P[12] are the most prevalent, while infections by strains with other genomic arrangements are infrequent. The identification of circulating strains of ERVA is critical for diagnostic and surveillance purposes, as well as to understand their molecular epidemiology. Current genotyping methods available for ERVA and rotaviruses affecting other animal species rely on Sanger sequencing and are significantly time-consuming, costly and labor intensive. Here, we developed the first one-step …

Gamete Nuclear Migration In Animals And Plants, Umma Fatema, Mohammad F. Ali, Zheng Hu, Anthony J. Clark, Tomokazu Kawashima

Plant and Soil Sciences Faculty Publications

The migration of male and female gamete nuclei to each other in the fertilized egg is a prerequisite for the blending of genetic materials and the initiation of the next generation. Interestingly, many differences have been found in the mechanism of gamete nuclear movement among animals and plants. Female to male gamete nuclear movement in animals and brown algae relies on microtubules. By contrast, in flowering plants, the male gamete nucleus is carried to the female gamete nucleus by the filamentous actin cytoskeleton. As techniques have developed from light, electron, fluorescence, immunofluorescence, and confocal microscopy to live-cell time-lapse imaging using …

The Effects Of A Bacterial Endotoxin On Behavior And Sensory-Cns-Motor Circuits In Drosophila Melanogaster, Oscar Istas, Abigail Greenhalgh, Robin L. Cooper

Biology Faculty Publications

The effect of bacterial sepsis on animal behavior and physiology is complex due to direct and indirect actions. The most common form of bacterial sepsis in humans is from gram-negative bacterial strains. The endotoxin (lipopolysaccharide, LPS) and/or associated peptidoglycans from the bacteria are the key agents to induce an immune response, which then produces a cascade of immunological consequences. However, there are direct actions of LPS and associated peptidoglycans on cells which are commonly overlooked. This study showed behavioral and neural changes in larval Drosophila fed commercially obtained LPS from Serratia marcescens. Locomotor behavior was not altered, but feeding …

A Generative Human-Robot Motion Retargeting Approach Using A Single Rgbd Sensor, Sen Wang, Xinxin Zuo, Runxiao Wang, Ruigang Yang

Computer Science Faculty Publications

The goal of human-robot motion retargeting is to let a robot follow the movements performed by a human subject. Typically in previous approaches, the human poses are precomputed from a human pose tracking system, after which the explicit joint mapping strategies are specified to apply the estimated poses to a target robot. However, there is not any generic mapping strategy that we can use to map the human joint to robots with different kinds of configurations. In this paper, we present a novel motion retargeting approach that combines the human pose estimation and the motion retargeting procedure in a unified …

Bovine Milk Extracellular Vesicles (Evs) Modification Elicits Skeletal Muscle Growth In Rats, Hailey A. Parry, Christopher Brooks Mobley, Petey W. Mumford, Matthew A. Romero, Cody T. Haun, Yufeng Zhang, Paul A. Roberson, Janos Zempleni, Arny A. Ferrando, Ivan J. Vechetti Jr., John J. Mccarthy, Kaelin C. Young, Michael D. Roberts, Andreas N. Kavazis

Physiology Faculty Publications

The current study investigated how bovine milk extracellular vesicles (EVs) affected rotarod performance and biomarkers of skeletal muscle physiology in young, growing rats. Twenty-eight-day Fisher 344 rats were provided an AIN-93G-based diet for 4 weeks that either remained unadulterated [EVs and RNA-sufficient (ERS; n = 12)] or was sonicated [EVs and RNA-depleted (ERD; n = 12)]. Prior to (PRE) and on the last day of the intervention (POST), animals were tested for maximal rotarod performance. Following the feeding period, the gastrocnemius muscle was analyzed at the histological, biochemical, and molecular levels and was also used to measure mitochondrial function and …