Digital Commons Network^™

Muscle Mass And Immune Function In The Senior Horse, Alisa Christina Herbst

Theses and Dissertations--Veterinary Science

Senior horses (≥ 15 years) represent up to one-third of the global equine population, and the proportion of old horses (≥ 20 years) in the U.S. has been steadily increasing. Aging is associated with a loss of skeletal muscle mass in horses, and while age-related muscle loss is comparingly well characterized in humans, little is currently known concerning underlying mechanisms, adverse outcomes, or the prevalence of low muscle mass in senior horses. One factor proposed to play a role in the development of age-related muscle atrophy in humans is inflamm-aging, a low-grade inflammation that affects elderly people and that has …

Vole Population Dynamics In Cover Crops Transitioning To Soybeans With Integrated Pest Management By Habitat Modification, Jena L. Nierman

Theses and Dissertations--Forestry and Natural Resources

The use of cover crops has been a wildly used method in rotational row crop production. Cover crops have minimized soil runoff and aided in maintaining nutrients in agricultural fields. Increased use of cover crops has seen a corresponding increase in the amount of damage done to soybeans by voles. Currently, there are no mitigation methods that successfully decrease vole populations in agricultural fields. The use of habitat manipulation as an integrated pest management solution has not been studied as a practical solution for vole population management. During 2019 and 2020, I tested the impacts of various cover crop termination …

Quantifying The Source And Pathway Of Dissolved Reactive Phosphate In Karst Drainage Of The Inner-Bluegrass, Cory Radcliff

Theses and Dissertations--Biosystems and Agricultural Engineering

In the Midwestern U.S. seasonal hypoxia experienced in the Gulf of Mexico and harmful algal blooms in inland freshwater ponds, lakes, and rivers are partly fueled by dissolved orthophosphate loadings from disturbed landscapes. Efforts to reduce dissolved reactive phosphate (DRP) loadings have had varying levels of success and have led to insufficient water quality improvements. Inefficiencies in conservation strategies can stem from poor understanding of phosphate source and flow pathway dynamics. This study focused on monitoring sources and flow pathways of dissolved reactive P in a karst agroecosystem with phosphatic limestone. We collected event water samples at the Camden Creek …

Extraction Of Micro- And Nano-Plastic Particles From Water Using Hydrophobic Natural Deep Eutectic Solvents, Jameson R. Hunter

Theses and Dissertations--Biosystems and Agricultural Engineering

The production of plastic and the amount of waste plastic that enters the environment increases every year. Synthetic polymers will gradually break down into particles on the micro- and nano-scale. The micro- and nano-plastics pose a significant ecological harm by transporting toxic chemicals and causing inflammation and cellular damage when ingested. Two common plastics are polyethylene terephthalate (PET) and Polystyrene (PS), and a newer bioplastic polylactic acid (PLA) that has become a popular alternative. Deep eutectic solvents are a recently discovered solvent composed of a hydrogen bond donor and hydrogen bond acceptor and have been proposed as a cheaper alternative …

Characterization And Upgrade Of Endocarp Biomass Derived Lignin To Value Added Products, Makua Vin-Nnajiofor

Theses and Dissertations--Biosystems and Agricultural Engineering

Lignin is an abundant biopolymer and a promising source of feedstock for high-value chemicals and energy materials, however, application methods of lignin into valuable products is yet to be fully explored. This study explores the valorization of lignin derived from lignin-rich endocarp biomass through the development of lignin-derived electrode materials. The chemical composition and cellular structure of the endocarps were characterized using HPLC and scanning electron microscopy (SEM) imaging. Mechanical properties of walnut and peach endocarps and preprocessing requirements were investigated using nanoindentation and specific energy consumption during grinding. Next, lignin was extracted from endocarps using deep eutectic solvent and …

Modeling Energy Flows In Floating In-Pond Raceways Utilizing Solar Power Back-Up, Bo Smith

Theses and Dissertations--Biosystems and Agricultural Engineering

The In-pond Raceway (IPR) is a novel option for production aquaculture, depending on water moving devices to provide constant flow. Device failure may result in catastrophic fish loss, requiring power backup systems to mitigate risk in case of power outages. Because these systems must be dependable and many suitable locations are remote, off-grid solar photovoltaic (PV) systems with battery storage have been considered since they eliminate need for utility power. Such systems can be hard to size and expensive. This study modeled system requirements using an energy balance to determine whether systems could withstand varying loads based on climatological conditions. …

Southern Pine Beetle-Specific Rna Interference Demonstrates No Effects On Nontarget Insects, Hannah Marie Hollowell

Theses and Dissertations--Entomology

The endemic southern pine beetle (SPB), Dendroctonus frontalis Zimmermann, is a highly destructive bark beetle that has devastated coniferous forests of the southern United States for decades. Traditional management strategies have been unable to respond effectively to largescale SPB outbreaks, prompting exploration of novel techniques including RNA interference (RNAi). Double-stranded RNAs (dsRNAs) are effective at triggering gene silencing and inducing mortality in SPB, but determining delivery methods and demonstrating specificity of the treatments is necessary before this technology may progress to deployment.

I evaluated three SPB-specific dsRNAs on model nontarget insects to assess potential lethal effects, sublethal effects such as …

Distribution And Diversity Of Heliothine And Other Lepidopteran Nudiviruses, Emrah Ozel

Theses and Dissertations--Entomology

Helicoverpa zea nudivirus 2 (HzNV-2) is the only known sterilizing and sexually-transmitted insect virus and causes pathological symptoms in H. zea reproductive tissues. HzNV-2 has features that make it a candidate as a H. zea (corn earworm) control agent, such as the ability to cause asymptomatic (latent) and symptomatic (lytic) infections and the ability to influence mating behavior of its host to favor virus spread. HzNV pathology has been studied and its genome sequenced, however, its prevalence in natural populations is largely unknown. In this study, we developed and used a low-cost PCR-based molecular survey to investigate HzNV-2 prevalence and …

Equine West Nile Virus In Kentucky: Characteristics Of Historical Testing And Analysis Of Causal Factors, Kaelyn Short

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

As the arbovirus with the highest incidence in both humans and horses in the United States, West Nile Virus (WNV) presents a significant risk to the equine population of Kentucky. Widespread infection has the potential to cause a significant economic impact to the state and long-term health complications for the horses. To better understand the burden of equine WNV, historical diagnostic testing archives the University of Kentucky Veterinary Diagnostic Lab were analyzed along with environmental and census data collected from additional sources. A total of 2146 test results were analyzed utilizing descriptive statistics, chi-square tests of independence, and binary logistic …

Accumbens Cholinergic Interneurons Mediate Cue-Induced Nicotine Seeking And Associated Glutamatergic Plasticity, Jonna M. Leyrer-Jackson, Michael Holter, Paula F. Overby, Jason M. Newbern, Michael D. Scofield, M. Foster Olive, Cassandra D. Gipson

Family and Community Medicine Faculty Publications

Nicotine, the primary addictive substance in tobacco, is widely abused. Relapse to cues associated with nicotine results in increased glutamate release within nucleus accumbens core (NAcore), modifying synaptic plasticity of medium spiny neurons (MSNs), which contributes to reinstatement of nicotine seeking. However, the role of cholinergic interneurons (ChIs) within the NAcore in mediating these neurobehavioral processes is unknown. ChIs represent less than 1% of the accumbens neuronal population and are activated during drug seeking and reward-predicting events. Thus, we hypothesized that ChIs may play a significant role in mediating glutamatergic plasticity that underlies nicotine-seeking behavior. Using chemogenetics in transgenic rats …

Applying Mixed-Effects Modeling To Behavioral Economic Demand: An Introduction, Brent A. Kaplan, Christopher T. Franck, Kevin Mckee, Shawn P. Gilroy, Mikhail N. Koffarnus

Family and Community Medicine Faculty Publications

Behavioral economic demand methodology is increasingly being used in various fields such as substance use and consumer behavior analysis. Traditional analytical techniques to fitting demand data have proven useful yet some of these approaches require preprocessing of data, ignore dependence in the data, and present statistical limitations. We term these approaches “fit to group” and “two stage” with the former interested in group or population level estimates and the latter interested in individual subject estimates. As an extension to these regression techniques, mixed-effect (or multilevel) modeling can serve as an improvement over these traditional methods. Notable benefits include providing simultaneous …

The Evaluation Of The Role Of Research Administrators In The Biomedical Research Grant Lifecycle And How Public Universities’ Policies Are Designed To Protect Funding, Megan Eder

MPA/MPP/MPFM Capstone Projects

There was a time in the not-so-distant past, where a simple infection had the opportunity to send an individual to an early grave. With advancements in biomedical research, medical professionals can treat simple infections as well as more complicated illnesses. Medical research has come a long way, but there is still a need for research in order to find, treat, and advance cures for illness and disease. Last year, during the COVID-19 pandemic, the entire world was waiting for researchers to create a vaccine to help slow the spread and get the world back to operating at a normal capacity. …

The State Of Woodford County’S Assistance Network, Katherine Connair

MPA/MPP/MPFM Capstone Projects

This study seeks to determine what the overall picture of assistance network serving the Woodford County region is. The goal was to determine if there are any observable gaps in the network and to have a clear understanding of what the assistance network is. Gaps were identified by studying the existing network, surveying network members, and examining publicly available resources. A Strengths, Weaknesses, Opportunities, and Threats (SWOT) analysis was conducted to analysis the whole network. Assistance for this study refers to programs/organizations that provide cash or in-kind benefits/services to people (U.S. Census Bureau). The assistance network is all of the …

Keep Lexington Beautiful Strategic Plan, Katherine Losekamp

MPA/MPP/MPFM Capstone Projects

As an affiliate of Keep America Beautiful, Keep Lexington Beautiful (KLB) encourages residents of Fayette County to engage in environmentally sustainable practices (Keep Lexington Beautiful, 2020). KLB was formerly a city commission of Lexington- Fayette Urban County Government (LFUCG) but in the winter of 2020 to 2021 broke off to form their own independent non-profit by the same name. During this process, they maintained about half of their original board members and hope in the future to expand their board to get more people from different perspectives involved. This project utilizes a short-term strategic plan to guide KLB through this …

Fluid Dynamics: Representations Of Water In Music, James E. Evans

Theses and Dissertations--Music

Water has remained a subject of all kinds of musical works since at least the middle ages. These musical works lack the concrete representational capacity of paintings, photographs, and films, relying instead on more abstract metaphorical constructs to convey water imagery. Current scholarship on water music typically centers on Romantic and Impressionist works and does not examine the process of signification by which musical signs portray water. The principal goal of this study is to determine how musical devices convey specific aspects of bodies of water and how such devices interact and contribute to musical depictions of streams, rivers, lakes, …

Selenium Removal And Pollution Swapping Potentials Of Passive Anaerobic Bioreactors Receiving Coal Slurry Impoundment Wastes, Md Anik Mahmud

Theses and Dissertations--Plant and Soil Sciences

Worldwide, selenium is a major contaminant of concern for coal and metal ore mining industries. In coal mining regions of the eastern USA, for example, billions of liters of wastewaters with elevated Se oxyanion concentrations (primarily SeO₄^2- and SeO₃^2-) are generated during coal washing which is often stored in > 700 man-made impoundments built into remote mountain valleys. Because impoundments are unlined systems, they tend to leak contents into nearby streams, which has detrimental effects on sensitive biota, particularly fish. The primary goal of this project was to evaluate the capacity of passive anaerobic bioreactors (PAB) …

Efficacy And Mechanism Of Acs811 In Tomato Immune Responses, Layne Ellen Harris

Theses and Dissertations--Plant and Soil Sciences

Biologicals are increasingly becoming an important part of sustainable integrated pest management in agricultural crop production and encompass a wide variety of products with varying degrees of efficacy and available research data. As biologicals become more integrated into commercial production systems, it is critical to rigorously evaluate biological product efficacy and mode(s) of action in crops. The better that biologicals are understood in terms of effective application, integration into commercial programs, and their specific mechanisms in crop growth and protection, then the greater the opportunity for increasing yields and food security.

ACS811 is a microbial fermentation-based biological product from Alltech …

Personality And Emotion For Virtual Characters In Strong-Story Narrative Planning, Alireza Shirvani

Theses and Dissertations--Computer Science

Interactive virtual worlds provide an immersive and effective environment for training, education, and entertainment purposes. Virtual characters are an essential part of every interactive narrative. The interaction of rich virtual characters can produce interesting narratives and enhance user experience in virtual environments. I propose models of personality and emotion that are highly domain independent and integrate those models into multi-agent strong-story narrative planning systems. I demonstrate the value of the strong-story properties of the model by generating story conflicts intelligently. My models of emotion and personality enable the narrative generation system to create more opportunities for players to resolve conflicts …

Expanding Social Network Modeling Software And Agent Models For Diffusion Processes, Patrick Vaden Shepherd

Theses and Dissertations--Computer Science

In an increasingly digitally interconnected world, the study of social networks and their dynamics is burgeoning. Anthropologically, the ubiquity of online social networks has had striking implications for the condition of large portions of humanity. This technology has facilitated content creation of virtually all sorts, information sharing on an unprecedented scale, and connections and communities among people with similar interests and skills. The first part of my research is a social network evolution and visualization engine. Built on top of existing technologies, my software is designed to provide abstractions from the underlying libraries, drive real-time network evolution based on user-defined …

Rad Modulation Of The L-Type Calcium Channel Confers Systolic Advantage In The Heart, Brooke Mildred Ahern

Theses and Dissertations--Physiology

Heart failure is a major public health problem and a leading cause of mortality. This clinical condition affects populations of all ages, and is the result of various cardiomyopathies. Almost half of these patients suffer specifically from heart failure with reduced ejection fraction; these hearts have decreased performance due to a failure of the heart to contract with sufficient force to meet demand. While there are therapies available to increase contractility, none of these enhance contraction without also further promoting pathological signaling and remodeling.

Under normal physiological conditions, the body elevates cardiac output through the fight-or-flight response. This response activates …

Myelin, Cpla2, And Azithromycin: Modulation Of Macrophage Activation In Spinal Cord Injury Inflammation, Timothy J. Kopper

Theses and Dissertations--Physiology

Spinal cord injury (SCI) produces a chronic inflammatory state primarily mediated by macrophages consisting of resident microglia and infiltrating monocytes. These chronically activated SCI macrophages adopt a pro-inflammatory, pathological state that continues to cause additional damage after the initial injury and inhibits recovery. While the roles of macrophages in SCI pathophysiology are well documented, the factors contributing to this maladaptive response are poorly understood. Here, we identify the detrimental effects of myelin debris on macrophage physiology and demonstrate a novel, activation state-dependent role for cytosolic phospholipase-A2 (cPLA₂) in myelin- mediated potentiation of pro-inflammatory macrophage activation. Macrophage- mediated inflammatory …

Dexamethasone Serum Concentrations After Intravenous Administration In Horses During Race Training, S. Mcclure, C. K. Fenger, K. Kersh, Bradley Brown, G. Maylin, W. Duer, L. Dirikolu, K. Brewer, Jacob Machin, Thomas Tobin

Veterinary Science Faculty Publications

Dexamethasone (DXM) sodium phosphate is a widely used corticosteroid for inflammatory conditions in horses, regulated in racing jurisdictions in the USA by a 0.005 ng/ml serum/plasma threshold. This study seeks to describe serum concentrations of DXM at 48 and 72 h after intravenous administration of 20 mg DXM sodium phosphate over 1 to 5 days, and to identify a possible source of DXM overages. 74 horses (39 Thoroughbreds, 13 Standardbreds, 22 Quarter Horses) in active race training received 20 mg DXM sodium phosphate. Serum was collected before injection, at 48 and 72 h post last injection, and analysed by LC/MS-MS …

Biocultural Evidence Of Precise Manual Activities In An Early Holocene Individual Of The High-Altitude Peruvian Andes, Fotios Alexandros Karakostis, Hugo Reyes-Centeno, Michael Franken, Gerhard Hotz, Kurt Rademaker, Katerina Harvati

Anthropology Faculty Publications

OBJECTIVES: Cuncaicha, a rockshelter site in the southern Peruvian Andes, has yielded archaeological evidence for human occupation at high elevation (4,480 masl) during the Terminal Pleistocene (12,500–11,200 cal BP), Early Holocene (9,500–9,000 cal BP), and later periods. One of the excavated human burials (Feature 15‐06), corresponding to a middle‐aged female dated to ~8,500 cal BP, exhibits skeletal osteoarthritic lesions previously proposed to reflect habitual loading and specialized crafting labor. Three small tools found in association with this burial are hypothesized to be associated with precise manual dexterity.

MATERIALS AND METHODS: Here, we tested this functional hypothesis through the application of …

Ancient Maya Rural Settlement Patterns, Household Cooperation, And Regional Subsistence Interdependency In The Río Bec Area: Contributions From G-Liht, Scott R. Hutson, Nicholas P. Dunning, Bruce Cook, Thomas Ruhl, Nicolas C. Barth, Daniel Conley

Anthropology Faculty Publications

Research on intensive agricultural features contributes to the social relations of farming, including the means by which farmers mobilize labor and the possible destination of surplus. Lidar provides high-resolution data on ancient houses and agricultural features at a regional scale. This paper uses lidar data from NASA’s G-LiHT airborne imager to derive insights about rural demography, interhousehold cooperation, and subsistence interdependency among the ancient Maya. We assess the differences in intensity of agricultural investment in rural and urban areas of the Río Bec region of southern Campeche and Quintana Roo, Mexico, leading to inferences about regional food exchange and complex …

Crouching Tiger Cello Concerto - A Melding Of Form And Content For The Concert Stage, Xiaohang Yu

Theses and Dissertations--Music

Tan Dun’s Crouching Tiger Concerto for Amplified Cello and Orchestra is not only one of the most frequently performed cello concerto of the recent past; it also demonstrates Tan’s masterful synthesis of artistic forms from the Chinese and the Western art music traditions with visual media that extends beyond the concert-hall. The music for this concerto was initially composed as part of the score for Ang Lee’s film Crouching Tiger, Hidden Dragon, itself a landmark blend of Chinese cinema with Western technique. The score broke boundaries, combining Western orchestral music with traditional Chinese instruments and thematic material. This melding …

Chimurenga Chepfungwa: A Liberated Cultural Curriculum For The Madzimbabwe Singer, Tanyaradzwa Tawengwa

Theses and Dissertations--Music

Chimurenga ChePfungwa is the self-liberation of the mind. Education can either be an instrument for liberation or a tool for colonial indoctrination, brainwashing, and enslavement — the European colonization of Africa in the late-nineteenth century and the continent's epic efforts at self-liberation have amply taught us this.

Chimurenga ChePfungwa calls for the centering of ChiVanhu knowledge systems such as Ngano, Tsumo neNziyo. ChiVanhu encapsulates Madzimbabwe indigenous knowledge systems, cosmologies, and ways of being, and this paper insists on the primacy of these ChiVanhu epistemologies for the construction of knowledge and knowing.

Amagama ayadala. Words create. So much of the language …

Entry And Replication Of Negative-Strand Rna Viruses, Kerri Boggs

Theses and Dissertations--Molecular and Cellular Biochemistry

Hendra virus (HeV) and human metapneumovirus (HMPV) are negative-sense, singled-stranded RNA viruses. The paramyxovirus HeV is classified as a biosafety level 4 pathogen due to its high fatality rate and the lack of a human vaccine or antiviral treatment. HMPV is a widespread pneumovirus that causes respiratory tract infections which are particularly dangerous for young children, immunocompromised individuals, and the elderly. Like HeV, no vaccines or therapies are available to combat HMPV infections. These viruses fuse their lipid envelopes with a cell to initiate infection. Blocking cell entry is a promising approach for antiviral development, and many vaccines are designed …

A Biochemical Characterization Of The Fusion Proteins From Enveloped Rna Viruses, Chelsea T. Barrett

Theses and Dissertations--Molecular and Cellular Biochemistry

Enveloped viruses must bind target cells and then fuse the viral membrane with a cell membrane to enter a host cell. These viruses use one or more surface glycoproteins to carry out these critical functions. The surface glycoprotein that carries out the fusion function, termed a fusion protein, is divided into three classes based on structural similarities. Some of the most studied human viral pathogens, such as human immunodeficiency virus (HIV), Ebola virus, influenza, measles, and the recently emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), possess class I fusion proteins. Following synthesis, class I fusion proteins associate as non-covalently …

Entry And Early Infection Of Non-Segmented Negative Sense Rna Viruses, Jean Mawuena Branttie

Theses and Dissertations--Molecular and Cellular Biochemistry

Paramyxoviruses, pneumoviruses, and other non-segmented negative sense (NNS) RNA viruses have historically been of public health concern. Although their genomes are typically small (up to 19kbs) they are able to inflict large-scale detrimental pathologies on host cells. Human metapneumovirus (HMPV) is a widespread pathogen and is a NNS RNA virus. HMPV results respiratory tract infections and is particularly dangerous for preterm infants, the elderly, and immunocompromised individuals. Other viruses within the NNS RNA virus order include the deadly Ebola, Hendra, and Nipah viruses (EBOV, HeV, and NiV), as well as the re-emerging measles virus (MeV). Despite their public impact, there …

Therapeutic Targeting Of Leukemia Stem Cells To Prevent T-Cell Acute Lymphoblastic Leukemia Relapse, Meghan G. Haney

Theses and Dissertations--Molecular and Cellular Biochemistry

The survival rate of T-cell Acute Lymphoblastic Leukemia (T-ALL) relapse is a dismal 10% of affected adults and 30% of children, largely due to the relapsed disease being more aggressive and treatment resistant than the initial disease. Relapse is thought to occur because conventional chemotherapies are unable to reliably eliminate a unique cell type known as leukemia stem (or propagating) cells (LSCs). LSCs are the only cells within the leukemia with the ability to self-renew and remake or replenish the ALL from a single cell. Currently, the pathways governing self-renewal in LSCs are largely unknown, precluding our ability to successfully …