Digital Commons Network^™

An Ecological Study Of Glyphosate Use And Non-Hodgkin’S Lymphoma, Dexter Corlett, Steven R. Browning

Theses and Dissertations--Public Health (M.P.H. & Dr.P.H.)

Glyphosate is currently the most widely used herbicide in the world. Initially thought to be non-carcinogenic in humans, in 2015 glyphosate was classified as a “probable carcinogen” by the International Agency for Research on Cancer due to several small epidemiological studies indicating a link between the pesticide and hematologic cancers, especially non-Hodgkin’s lymphoma (NHL). The current work is an ecological study using counties in Kentucky, Arkansas, and Iowa to compare glyphosate usage to NHL incidence using a multivariate Poisson regression. We found no significant correlation between glyphosate use and NHL incidence, though caution should be taken to draw significance from …

Effects Of Snake Fungal Disease On The Survival And Growth Of The Queensnake (Regina Septemvittata), Andrew Jerome Ibach

Theses and Dissertations--Forestry and Natural Resources

Having only emerged as a threat to snakes in 2006, relatively little is known of snake fungal disease’s (SFD) impacts on demographic processes, particularly survival and growth. I used data from an extensive capture-mark-recapture study to examine survival and growth in central Kentucky Queensnake (Regina septemivittata) populations. I found that diseased snakes and healthy snakes possessed similar monthly survival estimates (SFD positive 0.9687, 95% CI 0.8444 to 0.9944; SFD negative 0.8735, 95% CI 0.7518 to 0.9402) and that disease state transition probability from SFD negative to SFD positive, and SFD positive to SFD negative were also similar (N-P …

Supporting Stylized Language Models Using Multi-Modality Features, Chengxi Li

Theses and Dissertations--Computer Science

As AI and machine learning systems become more common in our everyday lives, there is an increased desire to construct systems that are able to seamlessly interact and communicate with humans. This typically means creating systems that are able to communicate with humans via natural language. Given the variance of natural language, this can be a very challenging task. In this thesis, I explored the topic of humanlike language generation in the context of stylized language generation. Stylized language generation involves producing some text that exhibits a specific, desired style. In this dissertation, I specifically explored the use of multi-modality …

An Investigation Of Hhv6'S Impact On The Cognitive Progression And Microglial Changes In An Alzheimer's Disease Cohort, Charles E. Seaks

Theses and Dissertations--Physiology

The role of herpesviruses and, more specifically, HHV6 in the development of Alzheimer’s disease (AD) and associated cognitive decline is still being investigated. High ubiquity and prevalence in the population have led to a high degree of skepticism about HHV6 as a potential contributor to cognitive decline and dementias. However, recent evidence related to another herpesvirus, herpes simplex virus 1, suggests that reactivation, not carriage, of the virus may be the key factor to explain the dissonance between the virus’ ubiquity and contributions to dementias. With that in mind, we set out to assess cases from the Sanders-Brown Center on …

The Hybrid Teacher: Expanding The Vocal Pedagogy Regime, Mary Joy Nelson

Theses and Dissertations--Music

The world of professional vocal training is becoming more and more eclectic and demanding in the United States. Academic music programs, and subsequently, vocal and vocal pedagogy programs are still predominantly classical in 2022, due to the American university system’s historical roots. In the 21st century, the commercial music industry is at an all-time high. However, in contemporary academia, vocal and vocal pedagogy degree programs remain primarily classical. In recent years, voice professionals have been discussing the implications of academic programs updating their programs and pedagogy in order to include professional CCM and hybrid training. Classical, CCM and hybrid singers …

Viola Music Written By Costa Rican Composers: Analysis Of Three Compositional Trends In Costa Rica, Karen Soto Huertas

Theses and Dissertations--Music

What could come from a country that just this past year celebrated 200 years of independence that was only discovered in 1502 and was under the yoke of the Spanish Empire for over 300 hundred years? What contribution to the art music community could this third-world country, Costa Rica, bring to the table? Specifically, what contributions could Costa Rica make to the viola repertoire, which status as a soloistic instrument was raised only 100 or so years ago? How could a small country contribute to the repertoire of this often-forgotten instrument? To answer these questions, I have embarked on research …

Momentary Endurance: The Effects Of Mouthpiece Pressure And Capillary Refill Time On Short-Term Muscle Recovery In Trumpet Players, Taylor Gustad

Theses and Dissertations--Music

The topic of proper mouthpiece pressure usage has been widely pushed off to the margins of trumpet pedagogy, only receiving the passing mention in a limited number of method books. This research paper contains a cross sectional view of the few, but most prominently used method books in the trumpet community that shows the prevalence of this issue, as well as steps to bring in new and useful information in this field. There are several aspects of mouthpiece pressure that should be studied; however, this paper focuses on its connection to momentary endurance and capillary refill time.

Momentary endurance is …

Constructing A Cultural Bridge: The Compositional Approach And International Impact Of Albert Mangelsdorff’S Now Jazz Ramwong, Michael Desousa

Theses and Dissertations--Music

In the period following World War II, countries such as Japan, Vietnam, and Thailand were under constant intellectual bombardment from global superpowers vying for political influence. West Germany, a newly established ally of Western democracy, was a small part of a global effort to spread this democracy to Asia. Working alongside Joachim-Ernst Berendt and the Goethe Institute, the jazz trombonist Albert Mangelsdorff arranged an album which sought to merge both Western European and Asian music. The album, Now Jazz Ramwong (1964), and accompanying fifty-concert tour helped pioneer a lasting cultural connection between Western Europe and Asia. The methodology behind Now …

Analysis Of Regulatory Options To Reduce Ethylene Oxide Emissions In Southern Louisiana, Claire Oyler

MPA/MPP/MPFM Capstone Projects

Toxic chemicals are dangerous when exposed directly to humans but are often vital for the production of goods and services. Ethylene Oxide is a carcinogenic gas used as a commercial sterilizer and emitted during the manufacturing process of common household goods. The term fence-line communities describes the residents living adjacent to facilities emitting toxic chemicals into the air. Several predominately minority, low-income fence-line communities in Southern Louisiana are exposed to higher than average levels of Ethylene Oxide from nearby manufacturing facilities. This raises environmental justice and overall public health concerns in the area. The Environmental Protection Agency (EPA) is charged …

Manipulating Species Diversity: Environmental Impacts In Row Crop, Livestock, And Grassland Agroecosystems, Alayna A. Jacobs

Theses and Dissertations--Plant and Soil Sciences

The diversity of living species in an agroecosystem affects both natural resources and agricultural production efficiency. The rise of managed plant monocultures has allowed agricultural yields to increase over time. However, simplifying agroecosystems affects the capacity of the system to sustainably provide clean air, water, and productive soils essential for continued food and fiber production. This dissertation manipulates species diversity in row crop and forage agroecosystems prevalent in the Eastern United States and tracks the associated environmental and agricultural production consequences.

In row crop agroecosystems, increasing plant diversity with winter cover crops has been suggested as a strategy to increase …

The Role Of Class Xi Myosin In Fertilization Of Arabidopsis Thaliana, Umma Fatema

Theses and Dissertations--Plant and Soil Sciences

Fertilization is the process of fusion of haploid male and female gametes to develop a new individual. In most animals, microtubules drive the migration of the female pronucleus toward the male pronucleus for fertilization. By contrast, the fertilization process in flowering plants is dependent on actin filament (F-actin) dynamics; F-actin, not microtubules, is responsible for sperm nuclear migration. In one of the female gamete cells, the central cell, constant F-actin meshwork inward movement towards the nucleus takes place to transfer the sperm nucleus for karyogamy. This inward F-actin dynamic is controlled by a unique ARP2/3 (Actin Related Protein 2/3)-independent WAVE/SCAR …

Blocking The Acquisition Of Ethanol-Induced Conditioned Place Preference With 11, 21-Bisphenyl-19-Norpregnane (Pt150) In Coturnix Quail, Mia Radevski

Theses and Dissertations--Psychology

Alcohol use disorder (AUD) has been associated with a dysregulated stress system. Therefore, regulating stress hormones has been investigated as a potential therapeutic target for AUDs. The purpose of the current study was to investigate whether a stress hormone receptor antagonist, PT150, would block the rewarding properties of ethanol. Quail were used as subjects because a conditioned place preference (CPP) apparatus that utilized visual cues was used, and quail readily attend to visual cues. Visual cues in the environment have been shown to become associated with alcohol effects and later induce craving. Starting on day one, quail were pretreated with …

The Role Of Affective Heterogeneity On Treatment Effects For Youth With Conduct Problems, Pevitr Singh Bansal

Theses and Dissertations--Psychology

Conduct problems (CP) are a class of disruptive and aggressive behaviors (e.g., aggression, vandalism) comprised of both oppositional defiant and conduct disorder. CP are highly heterogenous and one vital factor that parses out this heterogeneity is affect, specifically the affective traits of irritability (IRR) and limited prosocial emotions (LPE). The current study examined how IRR and LPE predict distinct aspects of treatment efficacy including (1) treatment response (i.e., magnitude of change from week 1 to week 5); (2) trajectories (i.e., shape of symptom change); and (3) time-out (i.e., behavioral and emotional reactions to time-out). Participants were 49 youth aged 7-12 …

Investigating Mechanisms Of Injury And Intervention In A Novel In Vitro Model Of Traumatic Brain Injury In Organotypic Hippocampal Slice Cultures, Julia Elaine Jagielo-Miller

Theses and Dissertations--Psychology

Traumatic brain injuries (TBIs) impact millions of individuals each year and can pose long term consequences. Despite numerous attempts, no medication has been approved by the FDA to treat TBIs. The causes of these failed trials are multifaceted, but in part can be attributed to the complex nature of TBIs, as well as a lack of sufficient pre-clinical data. In vitro models of TBI are an important tool to help understand the cellular changes seen following the injury, in a highly controlled environment. For the following experiments, a novel model of TBI was used to injure organotypic hippocampal slice cultures, …

The Role Of Shoc2 In Embryonic Development, Rebecca G. Norcross

Theses and Dissertations--Molecular and Cellular Biochemistry

The canonical ERK1/2 signaling cascade regulates cellular functions critical in vertebrate embryonic development such as proliferation, apoptosis, differentiation, and migration. Thus, its signals are controlled by a variety of mechanisms. Scaffold proteins are considered central to the mechanisms regulating the transmission of the ERK1/2 signals. Yet, their functions in development nor the molecular mechanisms by which they exert their control are not well understood.

This study focuses on the essential regulator of ERK1/2 signals during development – the scaffold protein Shoc2. Loss of Shoc2 leads to early embryonic lethality in mice and zebrafish. Germline mutations in the shoc2 gene result …

Structural Basis For Reversible Glucan Phosphorylation By Glucan Dikinases And Glucan Phosphatases, Tiantian Chen

Theses and Dissertations--Molecular and Cellular Biochemistry

Plants store glucose in the form of starch, an insoluble macromolecule that is synthesized and degraded diurnally. Glucan phosphatases play a critical role in starch breakdown, working with glucan dikinases in a cyclic phosphorylation and dephosphorylation. Herein, we present a novel glucan phosphatase Like SEX Four3 (LSF3) from Physcomitrella patens. Structural and enzymatic studies demonstrate that LSF3 is a dimeric glucan phosphatase that displays robust specific glucan phosphatase activity. Dimerization is shown to be essential for LSF3 stability and function. Structure-guided bioinformatic analysis shows that LSF3 is found in early land plants and green algae.

Building upon our understanding of …

An Integrated Computational Pipeline To Construct Patient-Specific Cancer Models, Daniel Plaugher

Theses and Dissertations--Mathematics

Precision oncology largely involves tumor genomics to guide therapy protocols. Yet, it is well known that many commonly mutated genes cannot be easily targeted. Even when genes can be targeted, resistance to therapy is quite common. A rising field with promising results is that of mathematical biology, where in silico models are often used for the discovery of general principles and novel hypotheses that can guide the development of new treatments. A major goal is that eventually in silico models will accurately predict clinically relevant endpoints and find optimal control interventions to stop (or reverse) disease progression. Thus, it is …

Deletion Of Period Genes Exacerbates Diet-Induced Obesity In Female, But Not Male, Mice, Elizabeth Kantra

Theses and Dissertations--Biology

Sex differences in obesity in mice are mediated by differential responses of circadian rhythms to high-fat feeding in males and females. Other studies also showed circadian Period genes regulate diet-induced obesity in mice. In this study, we investigated the role of the Period genes in regulating sex differences in obesity. Male and female C57BL/6J wild type, Per1/2 KO, and Per1/2/3 KO mice were housed in 12L:12D and fed high-fat diet for 12 weeks. We found a striking sex difference in obesity such that disabling the Period genes exacerbated adiposity in female, but not male, mice. Increased adiposity in female Period …

Understanding The Effects Of Embryonic Hyperglycemia On Retinal Development And Maintenance, Kayla Titialii-Torres

Theses and Dissertations--Biology

Hundreds of millions of people are affected by diabetes worldwide. Whether they are diagnosed with prediabetes or Type I or II diabetes, there are a variety of mechanisms in the pathogenesis of diabetes. Diabetes is a disease which consists of recurring states of hyperglycemia that can be difficult to manage due to either lack of insulin production or improper utilization of insulin. While these mechanisms of action differ, complications induced by diabetes occur in both poorly regulated Type I and II. Common complications of diabetes include nerve damage, kidney damage, and eye damage. Eye damage specifically is called diabetic retinopathy …

Identifying Epidermal Enriched Genes Required For Planarian Regeneration- Sp. Schmidtea Mediterranea, Pallob Barai

Theses and Dissertations--Biology

The outer epithelial layer covering an organism, commonly known as the epidermis, is crucial for maintaining homeostasis and for the wound healing processes after injury. The planarian epidermis allows flatworms to heal their wounds and virtually restore any missing tissues. Immediately after amputation, planarians contract their muscle and stretch their epidermis to heal the wound area. However, how the planarian epidermis coordinates with other tissues and mechanisms after the initial wound healing processes begins is not understood in detail. I hypothesized that epidermal cell stretching upon wound healing induces transcriptional changes that are required for effective regeneration. To test this …

Assessing Ear Pinna Repair In The Mrl/Mpj Mouse Strain, Fatemeh Safaee

Theses and Dissertations--Biology

The outcome of tissue repair varies across species. Vertebrates such as salamanders have the ability to not only heal scar-free but also completely regenerate lost appendages. In contrast, most mammals heal their wounds with fibrotic scarring. Understanding the key drivers of these divergent injury responses remains a major unanswered question in animal biology. Previous work with the Murphy’s Roth Large (MRL/MpJ) inbred mouse strain suggested they have the ability to rapidly close small (2mm) ear holes. While this ability was originally published as an example of regeneration, subsequent work by other groups suggested that it might represent something more akin …

The Role Of Protein Tyrosine Phosphatase Receptor Type F In Intestinal Homeostasis And Colorectal Cancer, Ashley T. Skaggs

Theses and Dissertations--Molecular and Cellular Biochemistry

Protein phosphorylation defines one of the most important regulatory mechanisms in cell signaling. PTPRF, protein tyrosine phosphatase receptor type F, belongs to the class I R2A subfamily of protein tyrosine phosphatases (PTP). The overall objective of this dissertation is to investigate the molecular mechanisms by which PTPRF regulates normal and cancer stem cells by controlling Wnt signaling.

Dysregulation of Wnt signaling promotes the initiation and progression of colorectal cancer (CRC). We first determined the functional important of PTPRF in regulating Wnt signaling in CRC. Combining cell culture, 3D tumor organoid and xenograft models, results from our study establish PTPRF as …

A Multidisciplinary Characterization Of The Enzymology And Biology Of Reversible Glucan Phosphorylation In Toxoplasma Gondii , Robert Murphy

Theses and Dissertations--Molecular and Cellular Biochemistry

Toxoplasma gondii is an opportunistic, protozoan parasite of all warm-blooded animals, infecting roughly one-third of humans worldwide. Humans acquire infections by consuming T. gondii tissue cysts in undercooked meat or from oocysts shed in cat feces. Encysted parasites convert into rapidly growing tachyzoites that disseminate throughout the body, defining the acute phase of infection. Under host immune pressure, tachyzoites convert into bradyzoites that populate tissue cysts found in CNS or muscle tissue and persist for the lifetime of the host, defining the chronic phase of infection. Tissue cysts are responsible for transmission via carnivory, but also possess the ability to …

Defining Patient Cohorts For Guiding Clinical Trials And Treatment In Lafora Disease: A Model For The Rare Disease Community, Katherine Janae Donohue

Theses and Dissertations--Molecular and Cellular Biochemistry

In the US, approximately 8000 rare diseases have been identified. Combined, rare diseases impact more than 30 million people in the U.S. alone, with 75% of those being children. However, research, funding, and therapeutic development for the rare disease community remains challenging because of the incredible diversity – not only between diseases, but often even within a single disease.

LD is an ultra-rare childhood dementia and epilepsy caused by mutations in one of two driver genes: EPM2A, which encodes for the glycogen phosphatase laforin, and EPM2B/NHLRC1, which encodes the E3 ubiquitin ligase malin. Children with LD …

Remote Sensing For Quantifying C3 And C4 Grass Ratios In Pastures, Jordyn Alyssa Bush

Theses and Dissertations--Plant and Soil Sciences

The following studies investigate the accuracy and practicality of exploiting the color dichotomy present between C₃ and C₄ grass species to estimate their respective proportions from drone or camera captured imagery. Understanding the proportions of C₃ and C₄ grasses in pastures is vital to sound decision making for livestock production. The ability to monitor these proportions remotely will also allow for large scale monitoring as well as detection of changes in botanical composition over time and in response to weather events, management, or climate change. A free green canopy cover (GCC) analyzing software, Canopeo, was used …

Effectiveness Of A Web-Based Smoking Cessation Training Program For Health Care Professionals Within An Acute Psychiatric Facility, Allie Carmichael

DNP Projects

Background: Tobacco use is the leading preventable cause of death and disease in the United States, with the prevalence remaining especially high among people with mental illness. Mental health providers often lack training in smoking cessation and feel uncomfortable addressing the issue with this patient population. Studies show that educational training offered to providers can lead to increased knowledge that improves the provider’s confidence and attitude about delivering smoking cessation treatment to patients. Mental health professionals must receive training on smoking cessation treatment modalities so they can effectively provide holistic care needed for recovery for patients with mental illness.

Purpose …

Regulatory Mechanisms In Arabidopsis Embryogenesis, Sanjay Joshi

Theses and Dissertations--Plant and Soil Sciences

Plants have amazing regeneration properties with single somatic cells, or groups of cells able to give rise to fully formed plants. One means of regeneration is Somatic Embryogenesis (SE), by which an embryonic structure is formed that “converts” into a plantlet. Somatic embryogenesis has been used as a model for zygotic processes that are buried within layers of maternal tissues. Understanding mechanisms of somatic embryo induction and development are important as a more accessible model for seed development. We rely on seed development not only for most of our caloric intake, but also as a delivery system for engineered crops …

An Evaluation Of Eragrostis Tef For Future Cultivar Development, Eric Wayne Luteyn

Theses and Dissertations--Plant and Soil Sciences

The many benefits of utilizing teff (Eragrostis tef) as small grain is well documented in journal publications. Over time the domestication of this species has become essential to the diets of the local population and the livestock living in the African highlands. Current research points to a high diversity of trait characteristics found in many teff populations and the potential utilization of these traits to increase desirable characteristics in both grain and forage varieties. With the overarching goal of adding to what is already known about teff, this study evaluates the different traits that are of interest to …

Understanding The Cellular And Physiological Mechanisms Of Fertilization And Early-Stage Seed Development, Mohammad Foteh Ali

Theses and Dissertations--Plant and Soil Sciences

Flowering plants have evolved a unique double-fertilization process. Two sperm cells fuse with two female gametophytic cells, the egg and central cells within the ovule, giving rise to the embryo and endosperm, respectively. Sperm cells in flowering plants are nonmotile and delivered in close proximity to the egg and central cells by the pollen tube. Flowering plants have established filamentous actin (F-actin) based sperm nuclear migration system for successful fertilization. Prior to fertilization, the female gamete forms a mesh-like structure of F-actin that shows constant inward movement from the plasma membrane periphery to the center of the cell where the …

Development Of Soluble Bioactive Poly(Beta Amino Esters) (Pβaes) For Post-Synthesis Processing, Kelley Wiegman

Theses and Dissertations--Chemical and Materials Engineering

Polymers have proven to be a viable platform to facilitate the controlled and targeted release for a variety of therapeutic agents. Polymeric prodrugs are a growing area of drug delivery in which active pharmaceutical ingredients are covalently linked to a polymeric form in an effort to enhance stability, delivery, and pharmacology. One naturally occurring class of active ingredients, known as polyphenols, can potentially offer a vast array of beneficial therapeutic properties. However, the therapeutic efficacy of these compounds is severely limited due to poor solubility and bioavailability as well as an oxidative and thermal instability. To improve the efficacy of …