Digital Commons Network^™

Do Magnesium Ions Have Similar Effects As Calcium Ions On Resting Membrane Potential?, Anthony Hana, Youngwoo Kim, Joy Bidros, Katie Neglia, Robin L. Cooper

Biology Faculty Publications

Maintaining a membrane electrical potential of biological cells is a dynamic process, as some cells have a continually changing potential, like pacemaker cells, while other cells may function with large or small changes in the membrane potential. Additionally, some cells may change their electrical potential when stimulated or inhibited by electrical signals, chemical compounds, or both—either simultaneously or episodically. The persistent leak of K⁺ through two-pore-domain potassium channels (K2P) and of Na⁺ through Na⁺ leak channels (NALCNs) and the action of pumps and exchangers are primarily responsible for maintaining a resting potential. Ca²⁺ ions are known …

Can Love Bloom On A Battlefield: An Analysis Of Queer Militaristic Fanfiction, Brandon Bost

Theses and Dissertations--Communication

It is noted that readers and fans hold unique and valid interpretations of texts, interpretations that become expressed in fan works like fan art, memes, or the subject of this study, fanfiction. This study performs textual analysis on queer fanfiction in Metal Gear Solid and Code Geass fandoms. It examines the unique relationship militarism holds with intimacy, and how intimacy intersects with violence and control in unique ways in these fan texts. Within these fanfics, militarism can serve as a barrier to intimacy, forcing relationships to adapt to militaristic structures within these fan interpretations.

Building Public Health Data Dashboards: Tutorial Playbook, Elwin Wu, Raymond Balise, Benjamin Katz, Daniel R. Harris, Matthew Bullard, Naleef Fareed, Marc Larochelle, Jennifer Villani

Institute for Biomedical Informatics Faculty Publications

Public health data dashboards have substantial potential to improve transparency, understanding, and decision-making at multiple levels, from individuals to public health practitioners and policymakers. However, creating effective dashboards presents many challenges. In this case-based tutorial on public health dashboard development, we share lessons learned from our experience developing data dashboards for the HEALing Communities Study (HCS), a National Institutes of Health (NIH)–funded, community-engaged intervention to deploy evidence-based practices to reduce opioid overdose deaths in 67 communities across 4 states. We present key decision points dashboard teams must address, along with the major considerations and trade-offs that shaped our approach. First, …

Chronic Alcohol Consumption Enhances The Differentiation Capacity Of Hematopoietic Stem And Progenitor Cells Into Osteoclast Precursors, Hami Hemati, Madison B. Blanton, Jude Koura, Rupak Khadka, Kathleen A. Grant, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Chronic alcohol consumption (CAC) is associated with an enhanced risk of bone fracture, reduced bone density, and osteoporosis. In a rhesus macaque model of voluntary ethanol consumption, CAC induces functional, transcriptomic, and epigenomic changes in hematopoietic stem and progenitor cells (HSPCs) and their resultant monocytes/macrophages, skewing them toward a hyper-inflammatory response. In the present study, those studies were extended to investigate alterations in osteoclast development, which, in postnatal life, differentiate from HSPCs and play a critical role in maintaining bone homeostasis. Spectral flow cytometry revealed a skewing of HSPCs toward granulocyte-monocyte progenitors in the CAC group, consistent with an increased …

Chronic Alcohol Consumption Reprograms Osteoclast Lineage Communications To Promote Osteoclastogenesis, Hami Hemati, Brianna M. Doratt, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Chronic alcohol consumption increases the risk of osteoporosis and fracture by disrupting bone remodeling, in part by enhancing osteoclastogenesis. However, the cellular mechanisms underlying this process remain incompletely defined. We analyzed scRNA-seq data from osteoclasts differentiated in vitro from bone marrow mononuclear cells obtained from macaques following 12 months of chronic ethanol or isocaloric control solution consumption. Module scoring, trajectory inference with generalized additive modeling (tradeSeq), and CellChat-based analyses of intercellular communication were applied to uncover ethanol-induced changes in metabolic reprogramming, lineage progression, and signaling network dynamics. Module scoring indicated metabolic reprogramming toward oxidative phosphorylation, with reduced glycolytic, migratory, and …

Alcohol Consumption Exacerbates High-Fat Diet- Mediated Disruptions In Myelopoiesis And Osteoclastogenesis In Mouse Models Of Metabolic Dysfunction-Associated Liver Diseases, Hami Hemati, Madison B. Blanton, Lauren Rutt, Nicholas Keiran, Rebecca Geron, Florence Lima, Rebecca L. Mccullough, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Objective: Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) and Metabolic Dysfunction-Associated Alcohol-related Liver Disease (MetALD) exhibit systemic immune abnormalities. Given that such immune dysregulation is closely linked to the skeletal complications frequently observed in MASLD and MetALD, we sought to comprehensively characterize the bone marrow hematopoietic compartment and its link to osteoclastogenesis.

Methods: We utilized bone marrow from mouse models of MASLD (high-fat diet, HFD) and MetALD (high-fat diet plus ethanol, HFD+EtOH), followed by flow cytometric analysis to phenotype hematopoietic stem and progenitor cells (HSPCs), as well as in vitro and in vivo assays to evaluate osteoclastogenesis.

Results: We found that …

Assessing Radio Transmitter Weight Effect And Evaluation Of Northern Bobwhite Chick Survival In Coastal North Carolina, Autumn S. Randall, Theron M. Terhune Ii, Matthew T. Springer, Darin J. Mcneil

Forestry and Natural Resources Faculty Publications

Studies on the earliest life stages are essential to our ecological understanding of avian demography; however, monitoring technologies that allow tracking of small birds are still limited in a variety of ways. One critical limitation, until recently, has been the development of methods for attaching transmitters to young birds with precocial development (e.g., Galliformes, Charadriiformes, etc.). The modified suture technique offers a means to attach transmitters to precocial chicks while accommodating rapid growth characterized by birds with precocial young. Although the sutured transmitter method was developed for northern bobwhite (Colinus virginianus) chicks, there remain key unknowns regarding best …

Chronic Alcohol Consumption Disrupts The Gut Microbial And Metabolic Landscapes, Madison B. Blanton, Ethan G. Napier, Katelyn E. Keen, Ethan V. Stuart, Isaac R. Cinco, Hami Hemati, Ronald C. Bruntz, Landon Wilson, Stephen Barnes, Rupak Khadka, Kathleen A. Grant, Ilhem Messaoudi

Pharmaceutical Sciences Faculty Publications

Introduction: Alcohol use disorder (AUD) increases incidence of infections, organ damage, and cancers. Aberrant inflammation is likely a driver of these adverse outcomes. Indeed, chronic alcohol consumption (CAC) rewires macrophages/monocytes toward a hyper-inflammatory phenotype. Prior studies showed increased gut permeability and dysbiosis. Translocation of host- and microbial-derived metabolites could trigger the hyper-inflammatory responses generated by macrophages/monocytes. However, the exact changes in these metabolites remain poorly defined due to confounders that complicate clinical studies and the differences between human and rodent gut microbiomes.

Methods: Here, we utilized a non-human primate model of ethanol self-administration to characterize alcohol-induced alterations in gut microbes …

Expression Pattern Of The Hifα-Family In Equine Chorioallantois During Pregnancy And Placental Pathology, Yatta Boakari, Hossam El-Sheikh Ali, Gustavo Henrique Doná Rodrigues Almeida, Kirsten E. Scoggin, Claudia Barbosa Fernandes, Sophia Panelli Marchio, Mats H. T. Troedsson, Ana Claudia Oliveira Carreira

Maxwell H. Gluck Equine Research Center Faculty Publications

Equine placental dysfunction impairs fetal growth and disrupts pregnancy outcomes. Despite many advances in diagnosis, the molecular pathophysiology of equine placentitis and premature placental separation remains poorly understood. However, the hypoxia-inducible factor (HIF-α) pathway is related to placental hypoxia, and its modulation in placental diseases has been shown in other species. Therefore, we hypothesized that in conditions marked by equine placental dysfunction, oxygen deprivation in the chorioallantois membrane triggers the activation of the HIF-α pathway, which is related to molecular alterations associated with placental insufficiency. Accordingly, we compared the expression of HIF1A, HIF2A, HIF3A, and HIF1AN genes in equine chorioallantois …

Fecal Microbiota Transplantation Modulates Gut Microbiota And Lipid Metabolism To Reduce Diarrhea In Low-Birth-Weight Neonatal Calves, Zhanhe Zhang, Duo You, Donglin Wu, Xintong Li, Yang Jia, David L. Harmon, Ming Xu

Animal and Food Sciences Faculty Publications

This study aimed to elucidate the mechanism by which colonic fecal microbiota transplantation (FMT) improves lipid metabolism in low-birth-weight neonatal calves via modulation of the gut microbial ecosystem. In a completely randomized design, seventy newborn Holstein calves (1 day old, 32.63 ± 3.13 kg BW) were assigned to two groups (35 calves/treatment; 30 females and 5 males per group). A healthy calf was used as a fecal donor. A 10% fecal bacterial suspension was prepared and administered colonically (50 mL, twice daily for 5 days). All calves were fed the same milk replacer and weaned using a step-down protocol at …

Wet Aging And Internal Endpoint Cooking Temperature Influence Color Of Lamb Longissimus Lumborum Muscle, Oluwatobi M. Ogunsola

Theses and Dissertations--Animal and Food Sciences

Color is a critical quality attribute that influences consumer purchase decisions for fresh and cooked lamb and is governed by myoglobin's redox chemistry. Wet aging is commonly used to enhance lamb tenderness, while endpoint cooking temperature is the primary determinant of internal cooked color. However, the combined effects of these factors on the color stability of the lamb longissimus lumborum (LL) muscle during refrigerated storage remain unclear. The objective of this thesis was to evaluate the effect of wet aging duration on the color attributes and lipid oxidation of fresh lamb LL chops and endpoint cooking temperature and refrigerated storage …

Combinatorial Immunotherapy Drives Exhaustion In Tumor Antigen–Specific Cd8+ T Cells Within The Mouse Renal Tumor Microenvironment, Holly R. Stephens, Elizabeth Elkins, Jing Li, Zoey N. Swalley, Henry Nnaemeka Ogbonna, Pia Muri, Zachary Roberts, Francesca R. Dempsey, Md Akkas Ali, Md Hasanul Banna Siam, Richard Kirkman, Jianqing Zhang, Sunil Sudarshan, Daniel J. Tyrrell, Hubert M. Tse, Daniel L. Smith Jr, Lyse A. Norian

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Immunotherapies have greatly improved outcomes for patients with renal cell carcinoma (RCC), yet response rates remain suboptimal and the factors promoting therapy resistance versus sensitivity are incompletely understood. Currently, no preclinical model of orthotopic renal cancer exists that permits evaluation of tumor antigen–specific (TAS) CD8⁺ tumor-infiltrating lymphocytes (TILs). To address this deficiency, we developed a mouse renal cancer model that permits tracking of adoptively transferred TAS CD8⁺ T cells. Renca-LUC tumor cells were transduced to express tumor ERK (tERK), a model antigen expressing a one-amino-acid change from wild-type ERK, resulting in recognition by tERK/H-2K^d-specific DUC Thy1.1 …

The Functional Significance Of Plk1 Phosphorylation Of Nrf2 In Prostate Cancer Progression, Maria Nur

Theses and Dissertations--Toxicology and Cancer Biology

Prostate cancer progression and therapeutic resistance are closely linked to alterations in redox homeostasis and cell cycle regulation. Nuclear factor erythroid 2–related factor 2 (NRF2) is a master regulator of antioxidant responses, yet the mechanisms integrating cell cycle signaling with NRF2 activity in prostate cancer remain poorly defined. In this study, we identify polo‑like kinase 1 (PLK1) as a novel regulator of NRF2 through direct phosphorylation at serine 253 (S253). Using bioinformatic prediction, mass spectrometry, in vitro kinase assays, and cell‑based analyses, we demonstrate that PLK1 phosphorylates NRF2 at S253 in a cell cycle–dependent manner, with maximal phosphorylation during mitosis. …

Impacts Of Livestock Dewormers On Dung Beetle Foraging Choices Across Spatial Scales, Madison L. Kluge

Theses and Dissertations--Entomology

Dung beetles provide ecosystem services in pasture systems but are vulnerable to chemical residues in livestock dung. Parasiticides containing macrocyclic lactones (MLs) are used to control parasites in livestock and are excreted unmetabolized in dung, exposing non-target organisms to these compounds. We used a multi-scale approach to examine whether ML residues alter dung beetle foraging ecology. At the field scale, we assessed dung beetle abundance and community composition using traps baited with dung from untreated cattle and cattle treated with ivermectin, moxidectin, or eprinomectin across eight farms and natural areas. At a smaller scale, we conducted behavioral assays to quantify …

Excess Nitric Oxide Alters Cellular Ph To Restrict Salicylic Acid Movement And Systemic Immunity, Fan Xia, Huazhen Liu, Keshun Yu, Xinyu Yuan, Aardra Kachroo, Pradeep Kachroo

Plant Pathology Faculty Publications

Plants carrying a mutation in S-nitrosoglutathione reductase 1 (GSNOR1) accumulate high nitric oxide (NO) and exhibit impaired immunity characterized by reduced pathogen-responsive salicylic acid (SA) accumulation and failure to activate normal SA signaling. We show that this reduced SA responsiveness in gsnor1 plants arises from impaired vascular-associated movement of SA, thereby compromising systemic acquired resistance (SAR). Elevated NO perturbs cellular pH homeostasis, acidifying the apoplast and alkalinizing the cytosol, which likely interferes with phloem-associated SA movement and renders gsnor1 plants unresponsive to foliar SA. In contrast, SA supplied via root drench restores SA signaling and SAR in gsnor1, …

Exploring Relational Farming Futures For Responsible Innovation In Precision Agriculture Through Q-Method And Speculative Design, Bhavna Joshi, Maaz Gardezi, Edward Prutzer, Awais Hameed Khan, Asim Zia, Benjamin E. K. Ryan, Ali Dadkhah, Donna M. Rizzo, John T. Mcmaine, David Clay

Biosystems and Agricultural Engineering Faculty Publications

This article draws on concepts of speculative design and responsible innovation to examine the possible impacts of data-driven precision agriculture on farmers and farm work. We use an innovative mixed-method approach to research design. Using artistic depiction of futures of precision agriculture in design workshop and in-depth semistructured interviews conducted with the farmers in Vermont and South Dakota, we explain how farmers perceive different futures of precision agriculture. Specifically, in our design workshop, we combined visual arts and social science methods, namely Q-method, to explore existing and new relationships between humans, technologies, and environment in the design, deployment, and use …

Temporal Evolution Of Brain Inflammation In The Bilateral Common Carotid Artery Stenosis Model Of Chronic Cerebral Hypoperfusion, Makayla F. Cox, Jill M. Roberts, Ryan K. Shahidehpour, David L. Dornbos Iii, Adam D. Bachstetter

Neuroscience Faculty Publications

Chronic cerebral hypoperfusion is a major contributor to vascular cognitive impairment and dementia, primarily through its disruption of white matter integrity and promotion of neuroinflammatory cascades. The bilateral common carotid artery stenosis (BCAS) model has emerged as the predominant experimental approach for studying the effects of chronic cerebral hypoperfusion, particularly in aging-relevant contexts. This review synthesizes current knowledge of inflammatory responses in the BCAS model, with an emphasis on their temporal progression and functional consequences. We begin by outlining the clinical burden of hypoperfusion, the rationale for BCAS, and the hemodynamic phases it produces. We then examine early neurovascular unit …

Dim Light At Night Impacts Circadian Rhythms And Alzheimer’S Disease-Like Neuroinf Lammation And Neuropathology In Humanized App Saa Knock-In Mice, Marilyn J. Duncan, Margaret R. Hawkins, Leke Bytyqi, Haleigh R. Whitlock, Savannah M. Shepard, Makayla F. Cox, Esther G. Drinkard, Teresa Macheda, Kelly N. Roberts, Katharina Kohler, Mary-Claire Schmidt, Carrie E. Johnson, Sridhar Sunderam, Bruce F. O'Hara, Michael Paul Murphy, Adam D. Bachstetter

Neuroscience Faculty Publications

Artificial light at night (light pollution) is widespread but understudied in the context of Alzheimer’s disease (AD). Sleep and circadian disruption have been linked to amyloid-β (Aβ) accumulation and neuroinflammation, but whether dim light at night (dLAN) modifies these processes remains unclear. We tested whether chronic dLAN exposure (8 lux during the dark phase, 8 weeks) alters circadian rhythms, amyloid pathology, and neuroinflammation in 12–13 month-old humanized APP knock-in (KI) mice. hAPP^SAA KI mice, which develop plaques, were compared with hAPP^WT KI controls carrying only a humanized APP sequence. dLAN reduced circadian rhythm amplitude and stability while increasing …

Brain-Derived Extracellular Vesicles Potentially Mediate Crosstalk With Peripheral Organs In Neurodegenerative Diseases, Ramzi H. Hamdalla, Vibha B. Bhaskar, Changhai Tian

Toxicology and Cancer Biology Faculty Publications

Brain-Derived Extracellular vesicles (BDEVs) are emerging mediators of intra- and interorgan communication in neurodegenerative diseases (NDs) such as Alzheimer’s Disease (AD) and Parkinson’s Disease (PD). A growing body of evidence suggests that BDEVs play an important role in modulating intercellular communication within the central nervous system in the pathogenesis of many NDs. By transporting non-coding RNAs (e.g., miRNAs) and important pathological proteins, BDEVs also influence peripheral organs and contribute to the progression of disease in the central nervous system (CNS). This review extends the understanding of NDs beyond solely brain dysfunction and gives a novel framework for the progression of …

Liposome-Encapsulated Clodronate And Cox-2 Inhibitor Treatment Impair Ventilatory Recovery But Improve Compensatory Locomotor Function Following Cervical Spinal Cord Injury In Rats, Aaron L. Silverstein, Chris M. Calulot, Christopher J. Mclouth, John C. Gensel, Warren J. Alilain

Spinal Cord and Brain Injury Research Center Faculty Publications

Over half of all spinal cord injuries (SCIs) in the United States occur at the cervical level and can cause locomotor deficits and life-threatening breathing dysfunction. Interestingly, the bisphosphonate drug clodronate has shown efficacy in ameliorating tissue damage and improving locomotor recovery acutely after experimentally induced thoracic SCI. Thus, we hypothesized that clodronate treatment would improve recovery of breathing and locomotor function following a C2 hemisection (C2Hx) model of cervical SCI in rats. Serendipitously, changes to animal use guidelines led to the inclusion of carprofen, a non-steroidal anti-inflammatory drug (NSAID), as another independent variable in our study. We treated adult …

Feeding The Family—A Food Is Medicine Intervention: Preliminary Baseline Results Of Clinical Data From Caregivers And Children, Gabriela Drucker, Christa Mayfield, Elizabeth T. Anderson Steeves, Sara Maksi, Tabitha Underwood, Julie Brown, Marissa Frick, Alison Gustafson

Behavioral Science Faculty Publications

Background/Objectives: Food is Medicine (FIM) programs have been shown to be effective at addressing food and nutrition insecurity among individuals. However, more evidence is needed to determine effective interventions at the household level and their impact on child health outcomes. Feeding the Family is a randomized controlled trial which aims to determine whether the amount of food provided and the ability to select foods in FIM interventions have an incremental effect on child and caregiver clinical outcomes relative to nutrition counseling alone. The objective of this paper is to describe the population at baseline among those enrolled in Feeding the …

A Ubiquitous Organophosphate Flame Retardant Disrupts Energy And Glucose Homeostasis In Mice, Krystal M. Sewell, Kelly Oriakhi, Sara Y. Ngo Tenlep, Megan L. Weaver, Mansura Shahad Bawa, Se-Hyung Park, Shubh Saraswat, Xiaohua D. Zhang, Robert N. Helsley, Samir Softic, Cetewayo S. Rashid

Pharmacology and Nutritional Sciences Faculty Publications

Organophosphate flame retardants (OPFRs) are used in many consumer products, and their presence in our environment raises concerns about potential health dangers. In humans, exposure to OPFRs, such as tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is associated with metabolic disorders such as obesity, hyperglycemia, and insulin resistance. Our current study seeks to determine how TDCPP exposure affects body composition, glucose homeostasis, and energy balance in a rodent model. C57Bl/6 J mice were divided into two weight-matched groups and fed purified low phytoestrogen diets containing either 0.1 % DMSO (control) or TDCPP 415 mg/kg resulting in an approximate exposure of 50 mg/kg/day. Both male …

Anatomical Atlas Of Kinase Responsiveness To Weight Gain: Adipose Depot Reprogramming In Diet-Induced Adiposity, Wang-Hsin Lee, Zachary A. Kipp, Sally N. Pauss, Genesee J. Martinez, Mei Xu, Terry D. Hinds, Jr.

Pharmacology and Nutritional Sciences Faculty Publications

Background/Objectives: Adipose tissue depots located at different anatomical sites exert differential functions in response to adiposity and glucose intolerance. These fat depots exhibit distinct metabolic signaling patterns that may influence pathological fat accumulation, thereby affecting the efficacy of anti-obesity interventions. Nonetheless, the mechanisms underpinning depot-specific signaling and pathway responsiveness remain insufficiently understood.

Methods: Kinase activity was characterized during the progression of adiposity across five adipose tissue depots in obese versus lean mice using the advanced PamGene kinome technology. Furthermore, kinase pathways in human preadipocytes and mouse 3T3-L1 preadipocytes were analyzed and compared with those in their differentiated, mature adipocytes. The …

Non-Contact Optical Spectroscopy For Metabolic And Vascular Characterizations Of Orthotopic Tongue Cancer Models In Vivo, Md Zahid Hasan, Jing Yan, Sumit Sarker, Pranto Soumik Saha, Caigang Zhu

Biomedical Engineering Faculty Publications

Most tissue optical spectroscopy platforms use a fiber probe for light delivery and collection, while the inconsistent probe-sample contact could induce significant distortions in the measured optical signals, which consequently bring analysis errors. Moreover, it will be practically difficult to use a fiber probe for measurements in some cases such as oral cancer investigations using small animal models. To address the critical challenge, we report a portable, lens-based, optical spectroscopy device capable of quantifying key vascular and metabolic parameters in vivo without probe-sample contact. We combined lenses based diffuse reflectance and fluorescence spectroscopy into one portable platform to enable multi-parametric …

Combined Autofluorescence And Diffuse Reflectance Spectroscopy For Rapid Metabolic And Vascular Characterizations Of Orthotopic Tongue Tumors In Vivo, Pranto Soumik Saha, Jing Yan, Sumit Sarker, Zahid Hasan, Caigang Zhu

Biomedical Engineering Faculty Publications

Precise label-free quantification of tissue metabolic and vascular dynamics in vivo represents a critical challenge for cancer therapy prediction and longitudinal treatment assessment. In this study, we demonstrated a portable autofluorescence and diffuse reflectance spectroscopy device along with novel spectroscopic algorithms to quantify tissue vascular and metabolic parameters of orthotopic head and neck cancer models in vivo. Tissue-mimicking phantom studies were used to verify the dual-modal optical spectroscopy and easy-to-use spectroscopic algorithms for rapid and accurate estimation of tissue oxygen saturation, total hemoglobin contents, and intrinsic optical redox ratio. Animal studies were conducted to demonstrate the feasibility of our …

Noninvasive Diffuse Optical Monitoring Of Cerebral Blood Flow And Oxygenation Responses To Intermittent Hypoxia In Neonatal Rats, Pegah Safavi, Mehrana Mohtasebi, Chowdhury Azimul Haque, Faezeh Akbari, Xuhui Liu, Yiqi Yuan, Li Chen, Lei Chen, Guoqiang Yu

Biomedical Engineering Faculty Publications

Significance: Intermittent hypoxia (IH) is common in preterm neonates and can cause hypoxic–ischemic brain injury. Simultaneous monitoring of cerebral blood flow (CBF) and oxygenation is essential to detect oxygen delivery-extraction mismatches and guide intervention.

Aim: We aimed to adapt and test an innovative diffuse speckle contrast flow oximetry (DSCFO) system for continuous monitoring of cerebral hemodynamics during IH in neonatal rats, a model approximating human neonates.

Approach: Two compact laser diodes and a miniature CMOS camera were integrated into a fiber-free probe for continuous monitoring of changes in relative CBF (rCBF) and oxy- and deoxy-hemoglobin concentrations (Δ[HbO₂] and …

Comparative Evaluation Of R134a And Hfo‐1234ze Cryogen Spray Cooling Using A Mouse Model With Controllable Epidermal Pigmentation, Wangcun Jia, Yurie Jeong, Crystal Huynh, Oswald Lai, Emily Nguyen, Grant Swajian, Jade Niu, Ayana Kashikar, Joshua Khaw, Hong Pu, Bernard Choi, John August D'Orazio, J. Stuart Nelson, Bahman Anvari

Pediatrics Faculty Publications

Objectives: Cryogen spray cooling (CSC) is critical for protecting the epidermis during laser dermatologic procedures. The widely used cryogen, R134a, has a high global warming potential (GWP), motivating interest in environmentally favorable alternatives such as HFO-1234ze. This study evaluated whether HFO-1234ze provides epidermal protection comparable to R134a during laser exposure and investigated the mechanistic basis underlying any differences in cooling performance.

Methods: Laser-induced epidermal injury in conjunction with two cryogens was assessed in K14-SCF Mc1r^e/e mice with pharmacologically controllable interfollicular epidermal pigmentation. Laser treatment sites were assigned fluences relative to baseline skin lightness (L*) to span subthreshold …

Using Behavioral Skills Training To Teach And Promote Generalized Self-Instruction For High School Students With Intellectual Disability, Elise J. Slavey

Theses and Dissertations--Early Childhood, Special Education, and Counselor Education

This study presents findings from a multiple probe design that evaluated the effects of Behavioral Skills Training (BST) as a method of teaching an increase in self-instruction in completing vocational tasks for individuals with intellectual disability. The research included three U.S. high school students with intellectual disability and the data were analyzed across conditions. Throughout the intervention of BST, generalization sessions were conducted alongside. All participants acquired self-instruction for the skill acquisition targets. However, only one participant was able to generalize self-instruction to novel vocational tasks. Results and suggestions for future research regarding generalization for self-instruction and procedure reliability data …

In Vivo Telemetry To Record Long-Term Cardiovascular Parameters, Temperature, And Activity In Spinal Cord Injury Rat Models, Sajeev Kaur, Anna M. Baur, Jillian A. Condrey, Dorottya P. Gal, Samir P. Patel, John C. Gensel

Spinal Cord and Brain Injury Research Center Faculty Publications

Higher-level spinal cord injury (SCI) above thoracic level 6 (T6) disrupts autonomic function and contributes to secondary complications, including fluctuations in blood pressure, heart rate, and temperature. In rats, the placement of telemetric implants in the descending aorta offers a robust methodology for assessing various cardiovascular parameters, such as systolic and diastolic pressure, mean arterial pressure, and heart rate. Core body temperature and animal activity can also be recorded following telemetric implant placement. A rat with a telemetric implant is kept on the receiver plate connected to a computer system for recording various parameters. Continuous long-term recordings at defined time …

Granulomatous Cellular Signatures In Nontuberculous And Tuberculous Mycobacterial Infections, Brianna M. Doratt, Ethan G. Napier, Mahdi Eskandarian Boroujeni, Sarah Douglas, Michael H. Davies, Luiz Bermudez, Eliot Spindel, Erin F. Mccaffrey, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Mycobacterium tuberculosis (Mtb) and nontuberculous mycobacteria (NTM) are acid-fast bacilli that trigger granuloma formation, a hallmark immune response aimed at containing infection. While the biology of Mtb granulomas has been widely investigated, far less is known about granulomas caused by NTM infection, despite the increasing prevalence and clinical challenge of NTM cases worldwide. Because granulomas influence infection control, pathology, and treatment response, understanding their cellular organization and signaling is critical to improving therapeutic strategies. Therefore, we characterized granulomas formed following infection of rhesus macaques with Mycobacterium avium subsp. hominissuis (MAH) and compared them to granulomas from cynomolgus macaques infected with …