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Neutral Detergent Fiber And Protein Levels In Diploid And Tetraploid Ryegrass Forage, L R. Nelson, F M. Rouquette Jr

IGC Proceedings (1977-2023)

Nutritive value of 18 ryegrass (Lolium multifloriumLam.) cultivars was studied at Overton, Te1

0.42-mm screen. Plots were harvested four times during each of the first 2 years and five times in the third year. In 1977-1978 and 1978-1979, NDF and protein analyses were run on samples from 2 replications. All forage samples were analyzed for pro­tein via the micro-Kjeldahl technique. Percentages of NDF were obtained via Van Soest extraction procedures. A seasonal increase in neutral detergent fiber (NDF) content and a decrease in protein percentage was observed. Differences in NDF were observed among cultivars and were most apparent during …

Forage Quality Of Barley Straw As Influenced By Genotype, D W. Meyer, D O. Erickson, A E. Foster

IGC Proceedings (1977-2023)

Cereal straw is an untapped carbohydrate source for ruminant animal feeds, but little information is available on genotypic differences in the feeding value of barleys (Hordeum vulgare L.) commonly grown in the northern Great Plains of the U.S.A. Our objective was to determine if barley genotypes differ in straw forage quality and if straw-quality parameters are associated with agronomic characters. Straw from 37 and 31 barley genotypes was sampled in 1977 and 1978, respectively. Straw from 10 barley genotypes grown at four locations was sampled in 1979. Standard laboratory procedures were used to determine the quality parameters measured. Correlations …

Development Of Gama Medic {Medicago Rugosa Desr.) As An Annual Leguminous Species For Dryland Farming Systems In Southern Australia, E J. Crawford

IGC Proceedings (1977-2023)

Annual medics (Medicago spp.) are the basic legume for nitrogen fixation in the alkaline soils of southern Australia. Not all in­dividual species are well adapted to all soil types, one difficult soil being the highly alkaline clay loam where annual rainfall is less than 500 mm. The first gama medic (M. rugosa) cultivar, Paragosa, was well adapted to the clay-loam soil type but proved to be susceptible to germination after summer thunderstorms, and seedlings died in the following drought. Many gama medic accessions were then grown in an attempt to select a genotype capable of maintaining seedcoat impermeability during …

Evolution Of Selection Techniques In Breeding For Bloat-Safe Alfalfa, B P. Goplen, R E. Howarth, G L. Lees, W Majak, J P. Fay, K J. Cheng

IGC Proceedings (1977-2023)

This report summarizes the Canadian breeding program to develop a bloat-safe alfalfa (Medicago sativa L.). This program has experienced an evolution of selection methods in attempting to identify bloat-safe alfalfa genotypes. In chronological order, the methods involved selection for fraction I (18S) protein, soluble proteins, foam volume, tannins (flavolans), saponins, initial rate of digestion (IRD), sonication, purified enzymes, gas production, and leaching. Although soluble herbage protein is correlated with the occurrence of pasture bloat, we have experienced slow progress in a selection program to reduce soluble-protein concentration in alfalfa. We have found that foam volume is not correlated with …

Value Of White Sweet Lupin In Production Of Protein Fodder And Feeding Of Animals, O Saric, I Ramosevac

IGC Proceedings (1977-2023)

The potential of white sweet lupin (Lupinus albus L.) as a grain or forage feed for livestock was investigated in a series of ex­periments. Grain yield of lupin was greater than or equal to that of soybeans (Glycine max [L.] Merrill) in most evaluations. The quality of lupin seed compared favorably with that of soybean meal and fishmeal. Lupin is capable of producing large amounts of forage in a short period, making it a good prospect for forage production in regions that have a short growing season.

White Clover: An Olde Crop With A Promising Future, P B. Gibson, W E. Knight, G W. Barnett, W A. Cope, J D. Miller

IGC Proceedings (1977-2023)

White clover (Trifolium repens L.) is a widely adapted, important forage legume for grazing in the U.S. The palatability and quality of the forage, the wide adaptation of the plants to growth in different climates and under various grazing conditions and the role it plays in supplying nitrogen to pastures make white clover our finest pasture legume. The use of adapted white clover cultivars should result in large economic gains to the livestock industry in the U.S .A. Beef and dairy farmers would profit from reduced need for nitrogen fertilizer, an extended grazing season, increased forage pro­duction and quality, better …

Performance And Variability Of Agronomic Characters In Populations Of Stylosanthes Guianensis (Aubl.) Sw., P S. Martins, N A. Vello

IGC Proceedings (1977-2023)

Stylosanthes guianens-is (Aubl.) Sw. shows wide variations in morphological and physiological characteristics, and basic infor­mation on genetic variability is needed for future breeding programs. The objectives of this paper were to evaluate 25 native Brazilian populations of S. guianensis for forage characteristics · • ;10,d • to estimate genetic and phenotypic parameters for these characters. The experiment was conducted in a duplicated 5 x 5 balanced lattice design with plots that contained 4 individuals spaced on 2-m centers. The following characters were evaluated after the beginning of flowering period: days to flowering (DF), mean diameter (MD), basal area (BA), growth …

Hedysarum Mongolicum Turcz.: An Important Protein Resource Legume On Dry Sandy Land, Li Min, A W. Hovin

IGC Proceedings (1977-2023)

In searching for plants that can both increase the protein production and improve the sandy-soil conservation of the grassland in the arid, windy regions of North China, so far, from among many native plants, we have identified Hedysarum mongolicum Turcz. as the best species to fulfill our dual purposes. We investigated its distribution, planted its native seeds in the Maowusu desert, and studied it under its native and cultivated growing conditions from 1957 to 1964. Chinese farmers call this perennial subbrush legume of the sweetvetcb genus "Yang Cai," meaning "sheep like to eat." It can thrive in the stable or …

Adaptation And Utilization Of Three Legume In China, Huang Wenhui

IGC Proceedings (1977-2023)

China is a vast territory with extremely varied climates, soil, and topography, and it is very rich in pasture grass resources. The main legumes for forage are Alfalfa (Medicago sativa L.), yellow alfalfa (Medicago folcata L.) and shadawang (Astragalis adsurgent Pall.)

Preliminary Evaluation Of Legume Germ Plasm In The Cerrados Of Brazil, D Thomas, R P. De Andrade

IGC Proceedings (1977-2023)

In 1978 an evaluation program was initiated to select legumes that (1) grow and produce seed on acid soils under aluminum and water stress, (2) persist under grazing, and (3) tolerate pests and diseases. The work is being conducted at the Cerrados Agricultural Research Center, near Brasilia, at lat 15°S and altitude 1,000 to 1, 100 m . Annual rainfall is 1,500 mm, mostly distributed in a 6-month wet season, and mean annual temperature is 21°C. Nine hundred legume accessions from thirteen genera have now been established as spaced plants on the 2 major soil types of the region. The …

A Testis-Specific Aralkylamine N-Acetyltransferase Regulates Dimorphic Sperm Function And Male Fertility In Moths, Hao Sun, Peng Yi Huang, Zhi Ruo Zhang, Cong Fen Gao, Subba Reddy Palli, Shun Fan Wu

Entomology Faculty Publications

Most Lepidopterans produce two distinct sperm types—nucleated eupyrene (fertile) and anucleate apyrene (non-fertile), yet the genetic mechanisms governing this dimorphism remain poorly understood. Using the fall armyworm (Spodoptera frugiperda) as a model, we identified a Lepidoptera-conserved, testis-specific arylalkylamine N-acetyltransferase (LTNAT) indispensable for male fertility. Functional analyses showed that LTNAT is expressed in both sperm types, and its depletion disrupts mitochondrial derivatives (MDs) in eupyrene sperm and drastically impairs apyrene sperm motility. Multi-omics analyses revealed LTNAT mutation perturbs energy metabolism, lipid homeostasis, and transmembrane transport—alterations correlating with abnormal MD structure. Furthermore, LTNAT deficiency likely compromises apyrene sperm motility by downregulating Sex-lethal …

Elevated Plasma Cholesterol Improves Sepsis Outcome By Promoting Hepatic Metabolic Reprogramming, Qian Wang, Jianyao Xue, Ling Guo, Dan Hao, Misa Ito, Rianna Reese, Bin Huang, Congqing Wu, Xiang An Li

Markey Cancer Center Faculty Publications

Background – Sepsis is a life-threatening condition with high mortality and limited therapeutic options. This study investigated the association between plasma cholesterol levels and sepsis survival and explored the mechanisms by which elevated cholesterol confers protection. Methods – We analyzed 2, 787 sepsis patients from the MIMIC-IV database, comparing cholesterol levels between 28-day survivors and non-survivors and assessing mortality risk using multivariable Cox regression. To test causality, C57BL/6J mice were fed either a high-cholesterol diet (HCD) or a regular diet (RD) before cecal ligation and puncture (CLP). Results – Survivors had significantly higher cholesterol than non-survivors (median 135 vs. 126 …

Postoperative Stress Accelerates Atherosclerosis Through Inflammatory Remodeling Of The Hdl Proteome And Impaired Reverse Cholesterol Transport, Dominique M. Boucher, Victoria Lorant, Valerie Rochon, Abigail Carter, Cameron Stotts, My Anh Nguyen, Thomas Laval, Christina Emerton, Nathan Joyce, Nysa Vinayak, Marlena Scaffidi, David Cook, Katey J. Rayner, Rebecca C. Auer, Scott M. Gordon, Mireille I. Ouimet

Physiology Faculty Publications

Over 10 million patients undergoing non-cardiac surgery each year face major cardiovascular complications within 30 days, many due to destabilized atherosclerotic plaques. Reverse cholesterol transport (RCT), driven by HDL and Apoa1, protects against plaque progression, but the effects of surgical inflammation on this pathway remain unclear. Using an abdominal laparotomy model in ApoE^−/− mice on a Western diet, we isolated the impact of surgical inflammation without confounding blood loss. Surgery acutely impaired RCT and cholesterol efflux, with inflammatory remodeling of HDL marked by elevated SAA1/2 and reduced Apoa1. Plaques exhibited higher intracellular lipids, PLIN2 expression, and cleaved caspase-3, indicating …

Lessons Learned: Commissioning An Fgd Wastewater Treatment System, Bella Triolet, Mckale Grant, Louis Manigault

World of Coal Ash Proceedings

Stantec recently supported commissioning a flue gas desulfurization (FGD) Wastewater Treatment System in accordance with the 2020 Environmental Protection Agency (EPA) Effluent Limitations Guidelines (ELG) Rule, which requires that coal fired power plants meet their more stringent requirements by December 31, 2025. With the addition of the 2024 EPA ELG Rule, coal fired power plants will now be expected to move to zero liquid discharge (ZLD) systems or permanently cease coal combustion by 2034. Due to this change, it is likely that coal fired power plants will be looking to design and install ZLD systems within the next decade. While …

Establishing Sustainable Vegetation On A Challenging Legacy Ccr Site, Derek Oosterhouse, Blake Arthur

World of Coal Ash Proceedings

American Electric Power (AEP) is closing and restoring a legacy Coal Combustion Residuals (CCR) site in Ohio that once held a power plant that pioneered innovative steam generation technology. The plant was closed in 1975. Today, the legacy CCR site is being transformed back to natural, sustainable vegetation. To comply with and close the Stormwater Pollution Prevention Plan (SWPPP) permit, permanent stabilization is required on the site. AEP, one of the largest electric companies in the country, holds a core principle to respect the environment through creative sustainable solutions. AEP connected with Profile Products, the world’s largest manufacturer and supplier …

Improving Economic And Environmental Outcomes Of Legacy Coal Ash Ponds, Grace Wilken

World of Coal Ash Proceedings

This project demonstrates a novel approach to utilize legacy coal ash waste ponds by processing the solids for use in cement/concrete and bioremediating the water fraction through algae cultivation. The algal biomass can then be used for animal feed, biofuels, or other valuable bio-products. There is an increasing demand for more environmentally friendly and cost-effective materials for concrete production, such as coal fly ash, yet a decreasing supply of ash as coal power plants are decommissioned in the transition to other energy sources. However, there are billions of tons of coal ash accumulated in legacy ash ponds across the US. …

Ensuring Quality Geosynthetics Installations Throughout The Project Lifecycle, Glen Toepfer

World of Coal Ash Proceedings

Geosynthetics serve numerous functions in containment systems used within the Coal Ash Industry and ultimately protect the site owner from liability. A failure in the geosynthetics containment system exposes the owner to liability; therefore, it is imperative that these geosynthetic containment systems be installed correctly to mitigate the risk of failure. Avoiding failure is not just the responsibility of the geosynthetics installer – it is the responsibility of every party involved in the project lifecycle from conception through post-closure. In fact, the success of the field installation is largely determined before the first panel of geomembrane is ever installed. The …

Mass Spectrometry-Based Spatial Imaging Of The Cochlea, Roberto A. Ribas, Qun Tang, Scarlett I. Caffee, Cameron J. Shedlock, Tara R. Hawkinson, Abigail K. Dragich, Borhane E.C. Ziani, Franca Bucco Paolasso, Alyson P. Black, Xin Ma, Harshitha C. Kota, Li Chen, Gregory I. Frolenkov, Derek B. Allison, Matthew S. Gentry, Ramon C. Sun, Craig W. Vander Kooi

Physiology Faculty Publications

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI) is transforming spatial molecular studies. However, applying MALDI-MSI to small, anatomically complex tissues remains challenging. One such structure is the cochlea, the auditory part of the inner ear that is critical for hearing. To address these challenges, we developed and implemented a streamlined workflow for sample preparation and processing to obtain MALDI-MSI data on mouse cochlea. Sample acquisition was optimized to minimize time and processing steps, allowing use of flash-frozen neonatal mouse heads. This workflow enabled high spatial resolution metabolomic and lipidomic imaging of the sagittally cryosectioned mouse cochlea using N-(1-naphthyl) ethylenediamine dihydrochloride …

Transportation Logistics Panel, John Ward, Andrew Vollmer, Tracy Zea, Marci Jenks

World of Coal Ash Proceedings

As the industry shifts from freshly generated ash to harvested materials and advanced utilization, logistics is becoming one of the primary drivers of project success or failure. This moderated panel will explore how sourcing, transportation, and storage realities are changing — and what project teams most often underestimate when working with harvested coal ash. Panelists will discuss what breaks, what becomes more complex, and how logistics considerations are reshaping planning and development.

The Age-Dependent Resident Myonuclear Multi-Omic Response To An Acute Skeletal Muscle Hypertrophic Stimulus In Mice, Pieter J. Koopmans, Ronald G. Jones, Ana Regina Cabrera, Francielly Morena, Nicholas P. Greene, John J. Mccarthy, Ahmed Ismaeel, Yuan Wen, Kevin A. Murach

Physiology Faculty Publications

A detailed analysis of how muscle fiber nuclei (myonuclei) respond to a hypertrophic stimulus could provide a critical step toward understanding compromised skeletal muscle plasticity with age. We used recombination-independent doxycycline-inducible myonucleus-specific fluorescent labelling, tissue RNA-sequencing, myonuclear DNA methylation analysis, multi-omic integration, and single myonucleus RNA-sequencing (smnRNA-seq) to define the molecular characteristics of adult (6–8 month) and aged (24 month) murine skeletal muscle after acute mechanical overload (MOV). In adult and aged MOV muscles, we found that: 1) similarities in the transcriptional response to loading—specifically in metabolism genes – were partly explained by a post-transcriptional microRNA-mediated mechanism that we corroborated …

Case Report: Gangrene After One Dose Of Investigational Stannic Pentetate Radiopharmaceutical, Zin W. Myint, Riham H. El Khouli, Bryan P. Lemieux, Charles A. Kunos

Radiology Faculty Publications

Introduction – Bone-seeking radiopharmaceuticals are the treatment of choice with clinical benefits for patients with extensive skeletal metastases, but they can also cause serious radiation-related adverse events (rrAEs). Management of rare and severe radiopharmaceutical rrAEs remains challenging because of incomplete knowledge of their natural history and pathogenetic mechanisms. We report a case of unlikely related gangrene following a protracted hospitalization after one dose of investigational [117mSn]tin-117m-diethylenetriaminepentaacetic acid (Sn-117m-DTPA) for treatment of extensive skeletal metastases from castration-resistant prostate cancer (mCRPC). Case presentation – An 80-year-old man with innumerable painful skeletal metastases from mCRPC developed anorexia and bilateral ankle edema with serous-draining …

Avoiding Misleading Estimates Of Among-Individual Variance Caused By Non-Random Sampling Of Individuals In A Changeable Environment, Denis Réale, Hassen Allegue, Yimen G. Araya-Ajoy, Ned A. Dochtermann, Shinichi Nakagawa, Joel L. Pick, Holger Schielzeth, David F. Westneat, Niels J. Dingemanse

Biology Faculty Publications

Animal ecologists frequently quantify variance in hierarchically structured traits in wild populations. Importantly, phenotypic plasticity within the period of measurement can modify the trait of interest in response to various unmeasured, temporally or spatially changeable, environmental conditions. Non-random sampling among units of the random effect (e.g. individuals) regarding the environment at issue may lead to estimates of the variance among ((Formula presented.)) or within ((Formula presented.)) such units that conflate several types of processes. This mixing of underlying biology can affect interpretations of the random effect variance. Here, we explore the conditions leading to this situation and assess potential solutions …

Mapping The Polar Neuro-Interactome Of Garcinia Mangostana Against The Ad-Pd-Als Nexus, Rahni Hossain, Sirirat Surinkaew, Pradoldej Sompol, Nasmah K. Bastaki, Rifat Jafrin, Nazim Sekeroglu, Jitbanjong Tangpong

Markey Cancer Center Faculty Publications

Background/Objectives: Neurodegenerative diseases like Alzheimer’s, Parkinson’s, and Amyotrophic lateral sclerosis (ALS) share common molecular pathways, including neuroinflammation and oxidative stress, which complicate the effectiveness of single-target treatments. Garcinia mangostana L. (mangosteen) has shown neuroprotective properties, but previous studies focused on lipophilic xanthones, which have poor bioavailability and uncertain blood–brain barrier permeability. Methods: In the current study, polar metabolites from G. mangostana peel aqueous extract (GMPE) were assessed for potential multi-target interactions via UHPLC-QTOF-MS-based metabolomics, systems pharmacology, and molecular docking analysis. Further, in silico ADMET screening and network-based analyses assessed for overlap between GMPE compounds and genes associated with neurodegeneration (AD, …

Systemic Consequences Of Chronic Ethanol Intake, Muni Swamy Ganjayi, Thomas A. Krauss, Gage E. Demster, Sehyung Park, Garrett B. Anspach, Sarah R. Anthony, Shaohua Wang, Michael Tranter, Robert N. Helsley, Cory W. Baumann

Markey Cancer Center Faculty Publications

Chronic ethanol (EtOH) consumption is a major contributor to multi-organ dysfunction, yet its systemic effects remain incompletely understood. To address this, we utilized a physiologically relevant long-term mouse model, administering 20% EtOH in drinking water for 60 weeks, to investigate the integrated consequences of chronic exposure. EtOH-consuming mice (0.4–0.5 mL/day) exhibited > 30% reductions in chow and fluid intake, resulting in a 12% decrease in total caloric intake compared to controls (p < 0.001). Body mass remained similar until Week 52, after which EtOH-treated mice had lower body mass due to reductions in both lean and fat mass (p ≤ 0.004). Functional assessments revealed impaired treadmill endurance (−17%) and grip strength (−11%) (p ≤ 0.037), while motor coordination remained unaffected (p = 0.203). Chronic EtOH exposure significantly altered gut microbiota composition, reducing Lactobacillus and enriching Faecalibaculum, Clostridium, and Bifidobacterium at the genus level. These changes were accompanied by marked depletion of short-chain fatty acids (p ≤ 0.05). Indirect markers of gut permeability (serum LPS & zonulin) and liver injury (serum ALT & AST, hepatic amyloid content) were elevated, alongside increased total cholesterol and > 62% upregulation of hepatic TNFα, IL-6 & serum amyloid A (p ≤ 0.046). EtOH also induced dyslipidemia and glucose intolerance (p ≤ 0.041), although transcriptomic changes in white adipose …

Domestic Guestworkers: A Call To End The H-2b Program And Empower American Workers, Preston Huennekens

Kentucky Law Journal

No abstract provided.

Federal Prosecution In The Shadows Of Constitutional Immunity, J. Richard Broughton

Kentucky Law Journal

No abstract provided.

Executive Immunity, Corruption, And The Rule Of Law: Death By A Thousand Cuts, Kimberly Breedon

Kentucky Law Journal

No abstract provided.

The Removal Responsibility, Kevin Frazier

Kentucky Law Journal

No abstract provided.

Dred Scott, Military Enslavement, And The Case For Reparations, Hugh Mcclean

Kentucky Law Journal

No abstract provided.

Building Jerusalem: Commercial Law And Ethics Of The Benefit Corporation, Jeff Lingwall, Susan Park

Kentucky Law Journal

No abstract provided.