Digital Commons Network^™

Tritium (3h) Radiolabeling Of Protein A And Antibody To High Specific Activity: Application To Cell Surface Antigen Radioimmunoassays, Ronald L. Wilder, Catherine C. Yuen, Subbarao Bondada, Virgil L. Woods, Cornelius B. Alexander, Rose G. Mage

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Staphylococcal protein A and several different immunoglobulins have been radio-labeled to high specific activities (> 106 cpm/pg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.

Effects Of Polychlorinated Biphenyl Compounds And Proposed Pcb-Replacement Products On Embryo-Larval Stages Of Fish And Amphibians, Wesley J. Birge, Jeffrey A. Black, Albert G. Westerman

KWRRI Research Reports

Static renewal bioassays were performed with four polychlorinated biphenyls, including Capacitor 21 and Aroclors 1016, 1242, and 1254. Each compound was used to treat embryo-larval stages of the rainbow trout (Salmo gairdneri), channel catfish (Ictalurus punctatus), goldfish (Carassius auratus), redear sunfish (Lepomis microlophus), leopard frog (Rana pipiens), Fowler's toad (Bufo fowleri), and American toad (Bufo americanus). Developmental stages of the trout were the most sensitive, with LC₅₀ 's at 4 days posthatching of 0.3, 1.0, 1.1, and 1.6 μg/l for Aroclors 1254, 1242, 1016, and …

Emission Of Microbial Aerosols From Polluted Waters In Densely Populated Regions, Lois S. Cronholm

KWRRI Research Reports

The air surrounding three activated sludge tanks was sampled over a two year period for the emission of bacterial aerosols under a variety of climatic conditions and at varying distances upwind and downwind of the aerated tanks. All plants emitted species of enteric bacteria which are significant as index organisms and as frank pathogens. The emission pattern of these bacteria were influenced by distance from the plant and wind direction. Within the parameters of a plant, defined arbitrarily in this study by sampling sites less than 150 m upwind and less than 900 m downwind, distance from the source was …

Energy In Agriculture: Grassland Renovation--Conserves Oil And Energy And Increases Returns From Grass Fields, Edward M. Smith, Larry W. Turner

Agricultural Engineering Energy Series

Fifty-four percent (1.2 billion acres) of land area in the 48 continental United States consists of grasslands, shrublands and open forests. For the most part, there is no alternative use of these land resources in production of food for man other than by way of ruminant animals. Sixty-nine percent (835 million acres) of these land resources is being utilized by grazing animals and forms the basis for a livestock industry which represents 43% of all agricultural receipts in the United States.

Energy In The Home: Energy Usage In Agricultural Production, John N. Walker

Agricultural Engineering Energy Series

Agriculture, even in its most primitive state, includes activities related to the collection and storage of solar energy in a form that can be used to sustain life. Energy must be expended in terms of human labor, animal labor, and fossil energy used for products and machinery utilized by agriculture. Because fossil energy is in short supply, many people are concerned, and rightfully so, about the effect that restricted energy availability will have upon agriculture production.

Influence Of Nitrogen Fertilization On The Quality And Quantity Of Streamflow From A Forested Watershed, George B. Coltharp, Michael T. Shearer, Everett P. Springer, Robert F. Wittwer

KWRRI Research Reports

This project was designed to determine the effects of nitrogen fertilization on the quality and quantity of streamflow eminating from an eastern hardwood forest watershed. A 40.67 ha watershed, located in mountainous eastern Kentucky, was aerially fertilized in late April 1975. The forest stand was principally oak, hickory, and yellow poplar, 50 - 55 years of age and in a relatively undisturbed condition. A helicopter applied anunonium nitrate at a rate of 504 kg/ha. Because a large part of applied nitrogen fertilizer ends up in the highly mobile nitrate nitrogen.form, this is the principal ion monitored in this study. No …

A Proposed Revision Of Kentucky's Water Rights Legislation, Richard Ausness

KWRRI Research Reports

Kentucky's present system of water law consists of a statutory water withdrawal permit system superimposed upon a body of common-law water rights doctrine. The rights of water users are often uncertain under this system, particularly in periods of water shortage. The proposed revision of Kentucky's existing water rights legislation, would greatly reduce the significance of common-law water rights and would remedy some of the weaknesses in the present statute.

Part 1 of the proposed statute establishes an administrative structure; Part 2 deals with water withdrawal permits; Part 3 retains the present statute's provisions on the regulation of dams and impoundments, …

Radiodiagnostic Agent, George A. Digenis, Manvendra B. Shambhu, Michael C. Theodorakis

Pharmaceutical Sciences Faculty Patents

A radiodiagnostic agent comprising a solid, insoluble nontoxic synthetic resin, such as polystyrene, bearing a detectable amount of technetium 99m thereon. This agent is useful for analyzing the gastrointestinal tract, for example, for gastric emptying time, by means of a gamma camera.

Energy Fact Sheet: Air-Type Solar Collectors For Agricultural And Residential Use, Blaine F. Parker

Agricultural Engineering Energy Series

This publication provides information on three basic air-type solar collectors for the purpose of explaining the characteristics of each. The collectors may be purchased assembled or constructed on the site. In either case an efficient collector of the right type should be selected for a given application.

Energy In Agriculture: Energy For Swine Facilities Part I: Energy Conservation, Robert L. Fehr

Agricultural Engineering Energy Series

Recently, there has been some concern about energy utilization in agricultural production. Shortages of natural and LP gas have affected many livestock producers, especially swine producers utilizing LP gas as a supplemental heat source in farrowing facilities. These shortages, coupled with an ever*increasing price for fuel and electricity, have encouraged many producers to begin looking for ways to reduce energy consumption.

Effects Of Soil Injection Of Liquid Dairy Manure On The Quality Of Surface Runoff, I. J. Ross, S. Sizemore, J. P. Bowden, C. T. Haan

KWRRI Research Reports

Liquid dairy manure has been injected on the soil contour to depths of 6 and 12 inches and applied to the surface of a Bluegrass sod and a bare tilled soil. Application rates of 9,250 gallons per acre were used. Runoff from 9-foot-square plots which were sprinkled at rates of 2.5 inches per hour on sod and 1.5 inches per hour on bare soil was collected and analyzed for various pollution parameters including COD, N, TS, TSS, pH, DO, and Fecal Coliform. The effects of pollutant yield in the runoff have been determined for various treatments.

Injection of the manure …

Removal Of Chloroform From Drinking Water, John S. Zogorski, George D. Allgeier, Robert Lee Mullins Jr.

KWRRI Research Reports

The overall objective of this investigation was to evaluate via laboratory experiments the technical feasibility of reducing trihalomethane levels in drinking water. Special attention was directed at the removal of chloroform since: (a) it is the only trihalomethane which has been shown to be carcinogenic in animal tests; and (b) this compound generally comprises the largest fraction of the total trihalomethane content of chlorinated waters in Kentucky. Trihalomethanes are present in municipal drinking waters due to the reaction of free chlorine with naturally occurring compounds, collectively called "precursors".

A variety of treatment processes and potential modifications (or additions) to existing …

College Of Law Commencement Program, 1978, University Of Kentucky

Graduation Programs

No abstract provided.

Development Of Procedures For Preparation Of The Interstate Safety Improvement Program, Jerry G. Pigman, Kenneth R. Agent, Charles V. Zegeer

Kentucky Transportation Center Research Report

This report summarizes background information and presents the development of procedures for preparing and updating the lnterstate Safety Improvement Program. There are two major sections: (1) development of procedures for identifying safety needs and (2) development of procedures for prioritizing safety needs. Related to the identification of needs are eleven subsections which are based on historical data, inventories of existing facilities, and analysis of current data. Several accident surveillance procedures were used to identify sites, sections, and elements of the roadway which warrant improvements. Procedures for prioritizing safety needs are based on summaries of data from two reports by the …

Studies On The Role Of Solar Ultraviolet Radiation In "Natural" Water Purification By Aquatic Ecosystems, John Calkins, Jeanne A. Barcelo, Perry Grigsby, Stephen Martin

KWRRI Research Reports

The possibility that pollution might deplete the stratospheric ozone layer and intensify solar UV at the earth's surface focuses attention on the role of solar UV in the various ecosystems at the earth's surface. Previous studies suggested that solar UV might contribute to bacterial die off in wastewater and the studies reported here were directed toward elucidating the action of solar UV in "natural" waters.

It has been assumed that solar UV action on aquatic ecosystems can be evaluated (using proper models) on the basis of the following four independently measurable quantities: I) the intensity of solar UV at the …

Water Use Permits In A Riparian State: Problems And Proposals, Richard C. Ausness

Law Faculty Scholarly Articles

Although adequate supplies of water are generally available in Kentucky, the law governing its use and allocation is much less satisfactory. At present, Kentucky water law is a complex mixture of common law and statutory water rights. The purpose of this article is to evaluate these water rights and suggest a number of improvements. Part I will examine the common law rules as they relate to both surface water and ground water. Part II will focus upon Kentucky's present system of statutory water use regulation and will identify some of its more serious deficiencies. Part III will recommend some short-range …

Kentucky’S Pavement Management System, James H. Havens, Eugene B. Drake

Kentucky Transportation Center Research Report

Pavement management concepts are discussed, and a method is presented illustrating the required data and its use to accumulate EAL's annually for comparison with the design EAL. This method can be used to determine overlay priorities, overlay design thicknesses, and financing schedules. A discussion of automatic feedback of field data is presented. Pavement condition reports should be analyzed separately to prevent improper adjustments in the design system due to causes of failure other than normal fatigue.

An overlay, whether for extending service life or improving skid resistance, provides an additional structural thickness and will modify the design life.

Preparation of …

Assessment Of The Water Quality In The Salt River Prior To Its Impoundment In Anderson And Spencer Counties, Kentucky, Andrew C. Miller, Louis A. Krumholz, Stuart E. Neff, Darly E. Jennings, Patricia B. Olmes, David S. White

KWRRI Research Reports

Monthly water samples were taken and analyzed to determine the water quality of the Salt River in Anderson and Spencer counties Kentucky prior to the river's impoundment. Sediments from the area watershed were analyzed to total acid digestion, barium chloride extraction, and aqueous extraction methods. Rainwater and runoff water were collected and analyzed for major cations and anions from two sites in the watershed.

The Salt River at Taylorsville is characterized by hard water with high levels of calcium (33.5-74.8 mg/1), bicarbonate (136-236 mg/l), specific conductance (200-535 μmhos/cm), and sulfate (16.5-71.5 mg/l). Nitrates (0.6-5.7 mg/l), phosphates (0.2-2.4 mg/l), sodium (3.2-20.3 …

Alfalfa Response To Varying Rates Of Phosphorus And Potassium Fertilization On Deep, Red, Limestone - Derived Soils Of The Pennyroyal Area In Kentucky, H. C. Vaught, Kenneth L. Wells, K. L. Driskill

Agronomy Notes

The Western Pennyroyal area of Kentucky consists largely of deep, well-drained limestone-derived soils occurring on an undulating to rolling landscape. A thin loess mantle overlies the limestone residuum in many areas. Soils on these landscapes are capable of good crop production, providing the basis of the rather intensive grain-hay- tobacco-livestock farming systems common to the Pennyroyal area. Because of the well-suited physical characteristics of these soils, alfalfa is a very important hay crop to this area.

The Review Of The College Of Law Alumni Association, Spring 1977, University Of Kentucky College Of Law

Annual Magazines

No abstract provided.

Southern Regional Beef Cow-Calf Handbook: Hay Feeding Systems, Garry D. Lacefield, J. Kenneth Evans, Joe Burns

Agriculture and Natural Resources Publications

Forages (pasture, hay and silage) constitute the most feed consumed by beef cattle. The most convenient approach in producing feed is to provide quality pastures during as much of the year as possible and permit animals to graze growing forage to the maximum. To provide a year-round feed supply, however, one must usually rely on stored feed when growth is inadequate to meet animal needs.

Hay is the most common source of stored feed used in most beef cattle operations. Nutrients provided in hay harvested at the proper stage of plant growth and undamaged by weather usually cost less to …

Hydrology And Water Quality In The Central Kentucky Karst: Phase 1, James F. Quinlan, Donald R. Rowe

KWRRI Research Reports

Study of springs and cave streams has shown that heavy metal-rich effluent from a wastewater treatment plant can be traced to Hidden River Cave (beneath the city of Horse Cave) and thence 4 to 5 miles north to a group of 39 springs at 14 locations along a 5-mile reach of Green River. Nickel, chromium, copper and zinc in these effluent-bearing springs are in concentrations of as much as 30 times greater than other springs upstream and downstream from this reach, 20 times greater than the Green River, and 60 times greater than in shallow domestic wells between Horse Cave …

The Eroding Uniformity Of The Uniform Commercial Code, John C. Minahan Jr.

Kentucky Law Journal

No abstract provided.

Embryopathic Effects Of Waterborne And Sediment-Accumulated Cadmium, Mercury And Zinc On Reproduction And Survival Of Fish And Amphibian Populations In Kentucky, Wesley J. Birge, Jeffrey A. Black, Albert G. Westerman, Paul C. Francis, Jarvis E. Hudson

KWRRI Research Reports

Fish and amphibian egg cultures were used to determine the embryopathic effects of cadmium, mercury, and zinc released from natural and metal-enriched sediments, and to develop egg culture bioassay procedures suitable for monitoring bottom sediments for hazardous contaminants. Eggs of the narrow-mouthed toad (Gastrophryne carolinensis), goldfish (Carassius auratus), and rainbow trout (Salmo gairdnei) were cultured in contaminant-free water added to natural and metal-enriched sediments. Exposure was initiated after fertilization (toad, goldfish) or at 10 days prehatching (trout) and maintained continuously through 4-10 days posthatching. Sediments were enriched with 0.1-100 ppm cadmium and mercury and …

Commonwealth V. Stephens: The Taking Doctrine At Work In Environmental Land Use Planning, Howell Hopson

Kentucky Law Journal

No abstract provided.

Doe V. Commonwealth's Attorney: A Set-Back For The Right Of Privacy, Tim O'Neill

Kentucky Law Journal

No abstract provided.

Law Vs. Science: Legal Control Of Genetic Research, Frank Becker

Kentucky Law Journal

No abstract provided.

A Challenge To Historic Preservation In Kentucky, Dale Deborah Brodkey

Kentucky Law Journal

No abstract provided.

Water Use Permits In A Riparian State: Problems And Proposals, Richard C. Ausness

Kentucky Law Journal

No abstract provided.

The Snail Darter V. The Tennessee Valley Authority: Is The Endangered Species Act Endangered?, Dale Deborah Brodkey

Kentucky Law Journal

No abstract provided.