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Role Of Calcium And Nitric Oxide Synthase (Nos) In Brain Mitochondrial Dysfunction, Vidya Nag Nukala

Theses and Dissertations--Neuroscience

Mitochondria are essential for promoting cell survival and growth through aerobic metabolism and energy production. Mitochondrial function is typically analyzed using mitochondria freshly isolated from tissues and cells because they yield tightly coupled mitochondria, whereas those from frozen tissue can consist of broken mitochondria and membrane fragments. A method, utilizing a well-characterized cryoprotectant such as dimethyl sulfoxide (DMSO), is described. Such mitochondria show preserved structure and function that presents us with a possible strategy to considerably expand the time-frame and the range of biochemical, molecular and metabolic studies that can be performed without the constraints of mitochondrial longevity ex vivo …

Functional Properties Of L-Glutamate Regulation In Anesthetized And Freely Moving Mice, Kevin N. Hascup

Theses and Dissertations--Neuroscience

L-glutamate (Glu) is the predominant excitatory neurotransmitter in the mammalian central nervous system with involvement encompassing learning and memory, cognition, plasticity, and motor movement. Dysregulation of the glutamatergic system is implicated in several neurological disorders including Parkinson’s disease, Alzheimer’s disease, Huntington’s disease and amyotrophic lateral sclerosis. The mechanisms underlying these neurological disorders are not clear, but evidence suggests that abnormal Glu neurotransmission plays a role. Elevated levels of Glu in the synaptic cleft overstimulate the N-methyl-Daspartate receptor leading to excitotoxicity, which causes neuronal loss in chronic neurological diseases. What is less understood is the source for the elevated Glu levels. …

Xenobiotic Transporters In Lactating Mammary Epithelial Cells: Predictions For Drug Accumulation In Breast Milk, Philip Earle Empey

Theses and Dissertations--Pharmacy

Recent literature has established that breast cancer resistance protein (ABCG2) is upregulated during lactation and is responsible for the greater than predicted accumulation of many drugs in breast milk. The objectives of this project were (1) to investigate the role of this transporter in the reported apically-directed nitrofurantoin flux in the CIT3 cell culture model of lactation, (2) to develop a mathematical model for drug transfer into breast milk to relate initial flux rates, steady-state concentrations, efflux ratios, and in vivo milk to serum ratios (M/S) and (3) to identify xenobiotic transporters that are highly expressed, and therefore potentially important …

The Role Of Macrophages In Olfactory Neurogenesis, Aaron S. Borders

Theses and Dissertations--Physiology

Olfactory sensory neurons (OSNs) undergo continual degeneration and replacement throughout life, a cycle that can be synchronized experimentally by performing olfactory bulbectomy (OBX). OBX induces apoptosis of mature OSNs, which is followed by an increase in the proliferation of progenitor basal cells. Macrophages, functionally diverse immune effector cells, phagocytose the apoptotic OSNs and regulate the proliferation of basal cells. This provides an advantageous environment to study how macrophages regulate neuronal death, proliferation, and replacement.

The purpose of this dissertation was to identify the cellular and molecular mechanisms by which macrophages regulate the degeneration/proliferation cycle of OSNs. Macrophages were selectively depleted …

"Appalachian Ingenuity" In Action: Activists Reach Beyond Traditional Economic Development In Kentucky, Stephanie Ann Blessing

University of Kentucky Master's Theses

This Thesis is an exploration into social change strategies in Appalachia that are alternative to conventional economic development practices and discourses. Drawing from original interviews with social justice activists in central and eastern Kentucky, I document a diversity of subversive discourses circulating in Appalachia, and I delineate models alternative to development that are driving action in several different communities. Through what one of my interviewees described as Appalachian ingenuity,1 individuals are enacting extremely hopeful and imaginative projects, and they are conjuring unique formulations that contribute to academic theories on alternative economies, capitalocentrism, neoliberalism, postmodern economics, anti-development, post-development, and spatial strategies …

Targeting Axon Growth From Neurons Transplantedinto The Central Nervous System, Kristine S. Ziemba

University of Kentucky Doctoral Dissertations

Damage to the adult mammalian central nervous system (CNS), either by traumatic injury or disease, usually results in permanent sensory and/or motor deficits. Regeneration of neural circuits is limited both by the lack of growthpromoting molecules and by the presence of growth-inhibitory molecules in the mature brain and spinal cord. The research described here examines the therapeutic potential of viral vectors and neuronal transplants to reconstruct damaged neural pathways in the CNS. Experimental neural transplantation techniques often fall short of expectations because of limited transplant survival and insufficient neurite outgrowth to repair connections and induce behavioral recovery. These shortcomings are …

Applying Reaction Time (Rt) And Event-Related Potential (Erps) Measures To Detect Malingered Neurocognitive Deficit, Victoria Louise Vagnini

University of Kentucky Doctoral Dissertations

This study examined the ability of reaction time (RT) and Event-Related Potentials (ERP) to detect malingered neurocognitive deficit (MNCD)in two new tasks compared to the TOMM (N = 47). Honest (HON), malingering (MAL), and traumatic brain injury (TBI) groups were compared on accuracy, RT and ERP measures. Overall, the Test of Memory Malingering (TOMM) accuracy was the most effective at classifying groups (hit rate = 100%). Several non-TOMM accuracy variables and RT variables reached hit rates in the range of 71%-88%. The TOMM RT variable had an unlimited time for participants to respond and was the most successful RT variable …

Abstract For A Look At Attitude And Achievement As A Result Of Self-Regulated Learning In The Algebra I Classroom, Darin Craig Schroeder

University of Kentucky Doctoral Dissertations

Not often do mathematics teachers instruct to improve students' attitudes toward mathematics. The pressures to cover the state-mandated curriculum drive teachers to instruct for procedural understanding with few connections. The lack of real-life connections results in students with low motivation toward mathematics and results in poor mathematics attitude (Ma andamp; Kishor, 1997). The purpose of this mixed-methods research is to examine self-regulated learning as an instructional technique aimed at increasing mathematical attitudes while also increasing achievement and to reveal barriers to its implementation in the classroom.The research study involved an intervention in a Mid-South urban high school at the 9th …

Detoxification Of Selected Chloro-Organics By Oxidation Technique Using Chelate Modified Fenton Reaction, Yongchao Li

University of Kentucky Doctoral Dissertations

The use of hydroxyl radical based reaction (Fenton reaction) for the destruction of organic pollutants has been widely reported in the literature. However, the low pH requirement and rapid hydrogen peroxide consumption rate make the application of conventional Fenton reaction difficult for in-situ treatment. In this study, we conducted a modified Fenton reaction by introducing a chelating agent into the reaction system that could prevent Fe(OH)3 (s) precipitation even at a neutral pH condition and reduce the H2O2 consumption rate by controlling the Fe2+ concentration. A chelating agent (mono-chelate or poly-chelate) combines with Fe2+ or Fe3+ to form stable metal-chelate …

Influences Of Chromium (Iii) Picolinate On Pigs Under Thermal, Immune Or Dietary Stress, And On Adrenal Steroid Secretion, Beob Gyun Kim

University of Kentucky Doctoral Dissertations

The objectives were to investigate the effects of chromium (III) picolinate (CrPic; up to 2,000 ppb of Cr) on growing pigs subjected to a variety of stressors including thermal, immune, or dietary stress and to examine the effects of CrPic on steroidogenesis from adrenocortical cells. In the thermal stress study, high ambient temperature caused reduced weight gain and feed consumption (P andlt; 0.01), and low ambient temperature caused increased feed intake and feed:gain (P andlt; 0.01). However, these effects were not moderated by CrPic, and respiratory rate, plasma cortisol, or plasma glucose were unaffected by CrPic. In the immune stress …

Trophic Interactions Between Two Herbivorous Insects, Galerucella Calmariensis And Myzus Lythri, Feeding On Purple Loosestrife, Lythrum Salicaria, And Two Insect Predators, Harmonia Axyridis And Chrysoperla Carnea, Bethzayda Matos, John J. Obrycki

Entomology Faculty Publications

The effects of two herbivorous insects, Galerucella calmariensis Duftschmid and Myzus lythri L. (Coleoptera: Chrysomelidae), feeding on purple loosestrife, Lythrum salicaria L. (Myrtiflorae: Lythraceae), were measured in the presence of two insect predators, Harmonia axyridis Pallas (Coleoptera: Coccinellidae) and Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae). A greenhouse cage experiment examined the direct effects of these predators on these herbivores, and indirect effects of predation on aboveground biomass, defoliation, number of leaves, and internode length. Eight treatment combinations with G. calmariensis, M. lythri, H. axyridis and C. carnea were applied to caged L. salicaria. The experiment ended …

Nonprofits: Recruiting And Retaining Baby Boom Volunteers In Central Kentucky. An Analysis Of Volunteer Needs In Central Kentucky And Best Practices For Recruiting Baby Boom Generation Volunteers, Ashley R. F. Moore

MPA/MPP/MPFM Capstone Projects

Statement of the Problem

Faced with tight budgets and expanding needs to serve and roles to play in the community, non-profit organizations have a shortage of volunteers. Especially desired are knowledgeable, passionate and dependable volunteers willing to serve on a regular basis. Although, the largest generation in our history, the Baby Boomers, which comprise 28% of American citizens and 77 million people, are retiring and acquiring increased free time, many are not being connected with volunteer opportunities. The Bureau of Labor Statistics reports that only one-third of those between ages 43 to 61 are serving as volunteers (US Bureau of …

Post-Traumatic Stress Disorder (Ptsd) In Children Following Acute Injuries Requiring Emergency Medical Care, Virginia Depp Cline

University of Kentucky Doctoral Dissertations

Unfortunately, one rite of passage of childhood is often serious injury that carries psychological impact along with the obvious physical repercussions. Prior studies have found conflicting results for protective/risk factors, thus this study attempted to explore PTSD in a sample of children ages seven to thirteen years of age with moderate to severe injuries. In this study (N = 32), 31.3% of children experiencing such a sudden injury requiring hospitalization at the University of Kentucky Childrens Hospital demonstrated significant indications of post-traumatic stress disorder (PTSD) following the injury. Several pre, peri, and post-trauma variables from during the childs hospitalization to …

Pet Ownership To Weight Status: A Path Analysis, Elizabeth Kingsley Casey

University of Kentucky Master's Theses

Research suggests that a multidimensional approach to obesity that addresses physiological, psychological, social, and environmental factors is optimal. Stress and self-esteem, as well as eating and exercise behaviors have been named as important factors in obesity. Curiously, although pets have been shown to reduce stress and are associated with higher self-esteem and cardiovascular health, the relationship between pet ownership and weight status has not been explored. A path analysis of the direct and indirect effects of pet ownership on stress, self-esteem, physical activity, eating behavior, and Body Mass Index failed to reveal a clear path linking pet ownership to weight …

Microelectrode Array Recordings Of L-Glutamate Dynamics In The Brains Of Freely Moving Rats, Erin Cathleen Rutherford

University of Kentucky Doctoral Dissertations

L-glutamate (Glu) is the predominant excitatory neurotransmitter inthe mammalian central nervous system (CNS) and is associated with a widevariety of functions including motor behavior and sensory perception. Whilemicrodialysis methods have been used to record tonic levels of Glu, little isknown about the more rapid changes in Glu signals that may occur in awakeanimals. We have previously reported acute recording methods using anenzyme-based microelectrode array (MEA) with fast temporal resolution (800msec), that is minimally invasive and is capable of detecting low levels of Glu (andlt;0.2 ??M) in anesthetized animals with little interference from other analytes. Wehave made a series of modifications …

Controlled Release Of Osteotropic Molecules Stimulates In Vitro Cellular Activity And In Vivo Local Bone Regeneration, Ju Hyeong Jeon

University of Kentucky Doctoral Dissertations

Bone defects treatment and reconstructive surgery continues to increase at a significant rate. Current bone defect treatments are autotransplantation, allograft, and xenografts create many problems such as, inflammation, infection and chronic pain. Moreover, allografts and xenografts arouse immune rejection. These problems have led to development of controlled release system for use as alternatives to autografts, allografts and xenografts in bone repair. There have been many approaches for sustained drug delivery in local bone regeneration using biodegradable polymers and osteotropic biomolecules. This dissertation presents new approaches that apply intermittent drug delivery for local bone regeneration. In the first, the osteotropic molecules …

Oxidative Dna Damage In Mild Cognitive Impairment And Late-Stage Alzheimer's Disease, Mark A. Lovell, William R. Markesbery

Chemistry Faculty Publications

Increasing evidence supports a role for oxidative DNA damage in aging and several neurodegenerative diseases including Alzheimer's disease (AD). Attack of DNA by reactive oxygen species (ROS), particularly hydroxyl radicals, can lead to strand breaks, DNA–DNA and DNA–protein cross-linking, and formation of at least 20 modified bases adducts. In addition, α,β-unsaturated aldehydic by-products of lipid peroxidation including 4-hydroxynonenal and acrolein can interact with DNA bases leading to the formation of bulky exocyclic adducts. Modification of DNA bases by direct interaction with ROS or aldehydes can lead to mutations and altered protein synthesis. Several studies of DNA base adducts in late-stage …

Novel Corn Hybrids For Silage Production, Warren Whitaker

University of Kentucky Master's Theses

Four corn (Zea mays) hybrid types at three plant densities and two nitrogen rates were evaluated for forage yield, forage quality and ensilage quality. The four hybrid types over the first two year of the study included nutri-dense, waxy, leafy, and dualpurpose, the third year a second dual purpose hybrid was added; while the three target plant densities were 54 000, 68 000, and 81 000 plants per hectare; and the nitrogen rates were 134 and 224 kilograms per hectare. The dual-purpose hybrid consistently had high forage yields compared with the other types. When averaged over nitrogen rate and hybrid …

The Impact Of Domestic Policies On International Competitiveness, Md. Ashfaqul Islam Babool

University of Kentucky Doctoral Dissertations

The impact of domestic policy regulations and standards on trade has been at the forefront of global policy during the past decade. Every country develops their own policies and standards that differ from country to country. These differences create problems for manufacturing industries, especially in major exporting countries. This study overviews the policy context driving standards in the manufacturing industries. The study consists of three different articles that attempt to examine the role of technical regulations and standards and their relationship with trade using different econometric models In the first article, the standard factor endowment approach is employed to explain …

Chiral 1, 2-Diamino Guests In Chain Replacement Peptidomimetics: A New Helical Motif, Marlon D. Jones

University of Kentucky Doctoral Dissertations

Peptides are short, sequence and length specific oligomers composed of small amino acid residues. Nature has refined these peptide sequences and their endogenous function through evolution. In addition, peptides have played an important role in medicine, which has lead to further research into developing peptides as lead pharmaceuticals (therapeutic peptides). Unfortunately, therapeutic peptides are inferior as drug candidates due to their low oral bioavailability; immunogenicity and potential to be attacked by peptidases. Fortunately, peptides can be modified by steric constraints, cyclization, and/or replacement of the peptide backbone itself creating a mimic (peptidomimetic) of the original peptide. Peptidomimetics are deliberately designed …

Hebi Sani: Mental Well Being Among The Working Class Afro-Surinamese In Paramaribo, Suriname, Aminata Cairo

University of Kentucky Doctoral Dissertations

This dissertation describes the results of a two year anthropological investigation into the concept of mental well being among the working class Afro-Surinamese population in Paramaribo, Suriname. More specifically, the research investigated how working class Afro-Surinamese in Paramaribo, Suriname define and maintain their sense of mental well-being, given their unique ethnic identity within a multi-ethnic and multicultural society, and given that their environment is heavily compromised by negative globalizing forces Over the course of two years a total of 62 people contributed information through group and individual interviews, which was supplemented by information obtained through participant observation. Findings show a …

New Developments In Cyclized Arsenic And Antimony Thiolates, Taimur A. Shaikh

University of Kentucky Doctoral Dissertations

There is a continued interest in the properties of arsenic thiolate compounds for both industrial and biological uses. Recent discoveries in the medicinal properties of such compounds have resulted in a sustained need for the synthesis of new dithiarsolane compounds for research as anti-leukemic compounds. Close analogues of the 2-halo arsenic dithiolates, namely those with an arsenic-carbon bond instead of an arsenic-halide bond, have recently been shown to have some efficacy towards leukemia cells. Based on the hydrolytic character and the active role of glutathione with arsenic in vivo, the compounds reported here may also have such activity. Arsenic compounds …

Projecting The Results Of State Smoking Ban Initiatives Using Cartographic Analysis, Donna Arlene Gilbreath

University of Kentucky Master's Theses

Because tobacco smoking causes 430,000 U.S. deaths annually, wide-reaching smoking bans are needed. Bans reduce cigarette consumption, encourage cessation, protect nonsmokers from second-hand smoke, and promote an attitude that smoking is undesirable. Therefore, bans may prevent future generations from suffering many smoking-related health problems. The federal government has not implemented widereaching smoking bans so it falls on individual states, counties, or communities to devise appropriate smoking policy. To date, smoking policy has been determined by legislators, who may have conflicts that prevent them from acting in the publics best interest. However, this method of implementing smoking policy may be changing. …

Neonatal Immune Modulation To Improve Pneumocystis Clearance, Kerry Mcgarr Empey

University of Kentucky Doctoral Dissertations

Pneumocystis carinii is an opportunistic fungal pathogen that causes lifethreatening pneumonia in immunocompromised individuals. Infants appear to be particularly susceptible to Pneumocystis (PC) pulmonary infections. The higher incidence of PC as well as other pulmonary infections among infants is likely due to an immature immune system. The neonatal lung environment is deficient immunologically in preterm as well as term infants (1, 2). Decreased phagocytic capacity of macrophages in newborns may increase the risk of infection from inhaled pathogens (1, 2). We have previously demonstrated that there is approximately a 3-week delay in the clearance of PC organisms from pup mouse …

Hair Coat And Steroidal Implant Effects On Steers Grazing Ndophyte-Infected Tall Fescue During The Summer, Linda Kay Mcclanahan

University of Kentucky Master's Theses

Sixty steers were grazed on toxic tall fescue for 104 days to determine the effects of hair coats and steroidal ear implants on physiological measurements. Steers were stratified by body weight and hair coat color for assignment to six, 3.0-ha pastures of 'Kentucky 31' tall fescue. Main plot treatments of either ten clipped or ten unclipped steers were randomly assigned to pastures. Five steers in each pasture were implanted with Synovex-S (200 mg progesterone-20 mg estradiol) and five were implanted with Compudose (25 mg estradiol) as sub-plot treatments. Hair growth rate averaged 0.29 mm/day. Sweating rate declined (P andlt; 0.001) …

Breeding For Value-Added Traits In Soft Winter Wheat, Carrie Ann Knott

University of Kentucky Doctoral Dissertations

Increased interest in value-added traits of soft winter wheat (SWW; Triticum aestivum L.), such as white-seed coat and gluten strength, has resulted from economic incentives for these traits. The first objective of this study was to determine whether differences existed between red- and white- seeded progeny of 17 populations. When abiotic and biotic stresses were negligible, significant differences were not detected between red- and white-seeded progeny, except for yield: red-seeded progeny had a significantly higher yield than the white-seeded progeny. However, when abiotic and biotic stresses were larger, the yield of white-seeded progeny was not significantly different from red-seeded progeny …

From Exception To Norm: Deaccessioning In Late Twentieth Century American Art Museums, Julianna Shubinski

University of Kentucky Master's Theses

Throughout their history in America, museums, including those of art, have adapted according to their environment. One result of this adaptability is that objects in art museum collections are not as permanent as those outside the museum field tend to believe. As scholarship, funding, and audiences change, objects which at one time were considered pertinent to a museum collection may be deaccessioned, the term used for when a museum removes an accessioned object from its permanent collection. Yet deaccessioning in America tended to remain the exception, rather than the rule, until the last three decades of the twentieth century. How …

Spectroscopy And Structures Of Cu-Organonitrogen Complexes, Xu Wang

University of Kentucky Doctoral Dissertations

Copper-organonitrogen complexes are studied by threshold photoionization and zero electron kinetic energy photoelectron spectroscopy. These complexes are prepared in pulsed laser vaporization supersonic molecular beams. Adiabatic ionization energies of the neutral species and vibrational frequencies of the neutral and ionic complexes were measured. Metal-ligand bond dissociation energies were obtained from the theoretical calculations or the experiments. More importantly, by combining the spectroscopic measurements, quantum chemical calculations, and spectral simulations, metal-ligand bonding structures are determined for copper complexes of diamines, pyridine, diazines, aminopyridines, polypyridines, and imidazole. The Cu-ethylenediamine, -(1,3-propanediamine), and -(1,4-butenediamine) complexes have been determined to be in a hydrogen-bond stabilized …

Approaches To Molecular Imprinting On Polysiloxane Scaffolds, Michael Edward Brown

University of Kentucky Master's Theses

Molecular imprinting, a common method used in separations and chromatography to isolate specific molecules via surface binding, has been adapted for applications in biomaterials and related sciences. The objective of this study was to determine the effectiveness of different approaches to molecular imprinting by testing for preferential binding of protein on polysiloxane scaffold surfaces. To test preferential rebinding, the scaffolds were exposed to a mixture of the template protein and a competitor protein with similar size but different chemistry. Lysozyme-imprinted polymers rebound 8.13 0.99% of lysozyme without any competition and 5.1 0.3% of the protein during competition. Lysozyme C peptide …

A Visualization Tool For Cross-Experiment Gene Expression Analysis Of C. Elegans, Lin Xue

University of Kentucky Master's Theses

Forty-six genomic gene expression studies of free living soil nematode C. eleganshave been published. To facilitate exploratory analysis of those studies, we constructed adatabase containing all the published C. elegans expression datasets. A Perl CGIprogram, called Microarray Analysis Display (MAdisplay), allows gene expressionclustergrams of any combination of entered genes and datasets to be viewed(http://elegans.uky.edu/gl/madisplay). Perl programs were used to preprocess the rawdata from different sources into a common format and to transform the data to displaythe expression changes relative to each experiment's controls. Three hundred lists ofgenes from figures and tables were extracted from the publications and made available inthe …