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Longitudinal Genetic Analyses Of Staphylococcus Aureus Nasal Carriage Dynamics In A Diverse Population, Gowrishankar Muthukrishnan, Ryan P. Lamers, Austin Ellis, Vanathy Paramanandam, Alana B. Persaud, Sergio Tafur, Christopher L. Parkinson, Alexander M. Cole Jan 2013

Longitudinal Genetic Analyses Of Staphylococcus Aureus Nasal Carriage Dynamics In A Diverse Population, Gowrishankar Muthukrishnan, Ryan P. Lamers, Austin Ellis, Vanathy Paramanandam, Alana B. Persaud, Sergio Tafur, Christopher L. Parkinson, Alexander M. Cole

Faculty Bibliography 2010s

Background: Staphylococcus aureus (SA) nasal colonization plays a critical role in the pathogenesis of staphylococcal infections and SA eradication from the nares has proven to be effective in reducing endogenous infections. To understand SA nasal colonization and its relation with consequent disease, assessment of nasal carriage dynamics and genotypic diversity among a diverse population is a necessity. Results: We have performed extensive longitudinal monitoring of SA nasal carriage isolates in 109 healthy individuals over a period of up to three years. Longitudinal sampling revealed that 24% of the individuals were persistent SA nasal carriers while 32% were intermittent. To assess …

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Anthracene-Polyamine Conjugates Inhibit In Vitro Proliferation Of Intraerythrocytic Plasmodium Falciparum Parasites, Jandeli Niemand, Pieter Burger, Bianca K. Verlinden, Janette Reader, Annie M. Joubert, Annette Kaiser, Abraham I. Louw, Kiaran Kirk, Otto Phanstiel Iv, Lyn-Marie Birkholtz Jan 2013

Anthracene-Polyamine Conjugates Inhibit In Vitro Proliferation Of Intraerythrocytic Plasmodium Falciparum Parasites, Jandeli Niemand, Pieter Burger, Bianca K. Verlinden, Janette Reader, Annie M. Joubert, Annette Kaiser, Abraham I. Louw, Kiaran Kirk, Otto Phanstiel Iv, Lyn-Marie Birkholtz

Faculty Bibliography 2010s

Anthracene-polyamine conjugates inhibit the in vitro proliferation of the intraerythrocytic human malaria parasite Plasmodium falciparum, with 50% inhibitory concentrations (IC(50)s) in the nM to mu M range. The compounds are taken up into the intraerythrocytic parasite, where they arrest the parasite cell cycle. Both the anthracene and polyamine components of the conjugates play a role in their antiplasmodial effect.

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Mapping Crmp3 Domains Involved In Dendrite Morphogenesis And Voltage-Gated Calcium Channel Regulation, Tam T. Quach, Sarah M. Wilson, Veronique Rogemond, Naura Chounlamountri, Pappachan E. Kolattukudy, Stephanie Martinez, May Khanna, Marie-Francoise Belin, Rajesh Khanna, Jerome Honnorat, Anne-Marie Duchemin Jan 2013

Mapping Crmp3 Domains Involved In Dendrite Morphogenesis And Voltage-Gated Calcium Channel Regulation, Tam T. Quach, Sarah M. Wilson, Veronique Rogemond, Naura Chounlamountri, Pappachan E. Kolattukudy, Stephanie Martinez, May Khanna, Marie-Francoise Belin, Rajesh Khanna, Jerome Honnorat, Anne-Marie Duchemin

Faculty Bibliography 2010s

Although hippocampal neurons are well-distinguished by the morphological characteristics of their dendrites and their structural plasticity, the mechanisms involved in regulating their neurite initiation, dendrite growth, network formation and remodeling are still largely unknown, in part because the key molecules involved remain elusive. Identifying new dendrite-active cues could uncover unknown molecular mechanisms that would add significant understanding to the field and possibly lead to the development of novel neuroprotective therapy because these neurons are impaired in many neuropsychiatric disorders. In our previous studies, we deleted the gene encoding CRMP3 in mice and identified the protein as a new endogenous signaling …

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Grape Extracts Inhibit Multiple Events In The Cell Biology Of Cholera Intoxication, Srikar Reddy, Michael Taylor, Mojun Zhao, Patrick Cherubin, Sandra Geden, Supriyo Ray, David Francis, Ken Teter Jan 2013

Grape Extracts Inhibit Multiple Events In The Cell Biology Of Cholera Intoxication, Srikar Reddy, Michael Taylor, Mojun Zhao, Patrick Cherubin, Sandra Geden, Supriyo Ray, David Francis, Ken Teter

Faculty Bibliography 2010s

Vibrio cholerae produces cholera toxin (CT), an AB(5) protein toxin that is primarily responsible for the profuse watery diarrhea of cholera. CT is secreted into the extracellular milieu, but the toxin attacks its Gs alpha target within the cytosol of a host cell. Thus, CT must cross a cellular membrane barrier in order to function. This event only occurs after the toxin travels by retrograde vesicular transport from the cell surface to the endoplasmic reticulum (ER). The catalytic A1 polypeptide then dissociates from the rest of the toxin and assumes an unfolded conformation that facilitates its transfer to the cytosol …

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A Vulnerability Assessment Of 300 Species In Florida: Threats From Sea Level Rise, Land Use, And Climate Change, Joshua Steven Reece, Reed F. Noss, Jon Oetting, Tom Hoctor, Michael Volk Jan 2013

A Vulnerability Assessment Of 300 Species In Florida: Threats From Sea Level Rise, Land Use, And Climate Change, Joshua Steven Reece, Reed F. Noss, Jon Oetting, Tom Hoctor, Michael Volk

Faculty Bibliography 2010s

Species face many threats, including accelerated climate change, sea level rise, and conversion and degradation of habitat from human land uses. Vulnerability assessments and prioritization protocols have been proposed to assess these threats, often in combination with information such as species rarity; ecological, evolutionary or economic value; and likelihood of success. Nevertheless, few vulnerability assessments or prioritization protocols simultaneously account for multiple threats or conservation values. We applied a novel vulnerability assessment tool, the Standardized Index of Vulnerability and Value, to assess the conservation priority of 300 species of plants and animals in Florida given projections of climate change, human …

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Smad-Pi3k-Akt-Mtor Pathway Mediates Bmp-7 Polarization Of Monocytes Into M2 Macrophages, Crystal Rocher, Dinender K. Singla Jan 2013

Smad-Pi3k-Akt-Mtor Pathway Mediates Bmp-7 Polarization Of Monocytes Into M2 Macrophages, Crystal Rocher, Dinender K. Singla

Faculty Bibliography 2010s

Previously we demonstrated that bone morphogenetic protein-7 (BMP-7) treatment polarizes monocytes into M2 macrophages and increases the expression of anti-inflammatory cytokines. Despite these findings, the mechanisms for the observed BMP-7 induced monocyte polarization into M2 macrophages are completely unknown. In this study, we demonstrate the mechanisms involved in the polarization of monocytes into M2 macrophages. Apoptotic conditioned media (ACM) was generated to mimic the stressed conditions, inducing monocyte polarization. Monocytes were treated with ACM along with BMP-7 and/or its inhibitor, follistatin, for 48 hours. Furthermore, an inhibitor of the PI3K pathway, LY-294002, was also studied. Our data show that BMP-7 …

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Electric Organ Discharge Diversity In The Genus Gymnotus: Anatomo-Functional Groups And Electrogenic Mechanisms, A. Rodríguez-Cattáneo, P. Aguilera, E. Cilleruelo, W. G. R. Crampton, A. A. Caputi Jan 2013

Electric Organ Discharge Diversity In The Genus Gymnotus: Anatomo-Functional Groups And Electrogenic Mechanisms, A. Rodríguez-Cattáneo, P. Aguilera, E. Cilleruelo, W. G. R. Crampton, A. A. Caputi

Faculty Bibliography 2010s

Previous studies describe six factors accounting for interspecific diversity of electric organ discharge (EOD) waveforms in Gymnotus. At the cellular level, three factors determine the locally generated waveforms: (1) electrocyte geometry and channel repertoire; (2) the localization of synaptic contacts on electrocyte surfaces; and (3) electric activity of electromotor axons preceding the discharge of electrocytes. At the organismic level, three factors determine the integration of the EOD as a behavioral unit: (4) the distribution of different types of electrocytes and specialized passive tissue forming the electric organ (EO); (5) the neural mechanisms of electrocyte discharge coordination; and (6) post-effector mechanisms. …

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Mycobacterial Trehalose Dimycolate Reprograms Macrophage Global Gene Expression And Activates Matrix Metalloproteinases, Kaori Sakamoto, Mi Jeong Kim, Elizabeth R. Rhoades, Rachel E. Allavena, Sabine Ehrt, Helen C. Wainwright, David G. Russell, Kyle H. Rohde Jan 2013

Mycobacterial Trehalose Dimycolate Reprograms Macrophage Global Gene Expression And Activates Matrix Metalloproteinases, Kaori Sakamoto, Mi Jeong Kim, Elizabeth R. Rhoades, Rachel E. Allavena, Sabine Ehrt, Helen C. Wainwright, David G. Russell, Kyle H. Rohde

Faculty Bibliography 2010s

Trehalose 6,6'-dimycolate (TDM) is a cell wall glycolipid and an important virulence factor of mycobacteria. In order to study the role of TDM in the innate immune response to Mycobacterium tuberculosis, microarray analysis was used to examine gene regulation in murine bone marrow-derived macrophages in response to 90-mu m-diameter polystyrene microspheres coated with TDM. A large number of genes, particularly those involved in the immune response and macrophage function, were up-or downregulated in response to these TDM-coated beads compared to control beads. Genes involved in the immune response were specifically upregulated in a myeloid differentiation primary response gene 88 (MyD88)-dependent …

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Immunomodulation And T Helper Th1/Th2 Response Polarization By Ceo2 And Tio2 Nanoparticles, Brian C. Schanen, Soumen Das, Christopher M. Reilly, William L. Warren, William T. Self, Sudipta Seal, Donald R. Drake Iii Jan 2013

Immunomodulation And T Helper Th1/Th2 Response Polarization By Ceo2 And Tio2 Nanoparticles, Brian C. Schanen, Soumen Das, Christopher M. Reilly, William L. Warren, William T. Self, Sudipta Seal, Donald R. Drake Iii

Faculty Bibliography 2010s

Immunomodulation by nanoparticles, especially as related to the biochemical properties of these unique materials, has scarcely been explored. In an in vitro model of human immunity, we demonstrate two catalytic nanoparticles, TiO2 (oxidant) and CeO2 (antioxidant), have nearly opposite effects on human dendritic cells and T helper (T-H) cells. For example, whereas TiO2 nanoparticles potentiated DC maturation that led towards T(H)1-biased responses, treatment with antioxidant CeO2 nanoparticles induced APCs to secrete the anti-inflammatory cytokine, IL-10, and induce a T(H)2-dominated T cell profile. In subsequent studies, we demonstrate these results are likely explained by the disparate capacities of the nanoparticles to …

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Novel Technique For Generating Macrophage Foam Cells For In Vitro Reverse Cholesterol Transport Studies, Bhaswati Sengupta, Chandrakala Aluganti Narasimhulu, Sampath Parthasarathy Jan 2013

Novel Technique For Generating Macrophage Foam Cells For In Vitro Reverse Cholesterol Transport Studies, Bhaswati Sengupta, Chandrakala Aluganti Narasimhulu, Sampath Parthasarathy

Faculty Bibliography 2010s

Generation of foam cells, an essential step for reverse cholesterol transport studies, uses the technique of receptor-dependent macrophage loading with radiolabeled acetylated LDL. In this study, we used the ability of a biologically relevant detergent molecule, lysophosphatidylcholine (lyso-PtdCho), to form mixed micelles with cholesterol or cholesteryl ester (CE) to generate macrophage foam cells. Fluorescent or radiolabeled cholesterol/lyso-PtdCho mixed micelles were prepared and incubated with RAW 264.7 or mouse peritoneal macrophages. Results showed that such micelles were quite stable at 4 degrees C and retained the solubilized cholesterol during one month of storage. Macrophages incubated with cholesterol or CE (unlabeled, fluorescently …

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Automatic B Cell Lymphoma Detection Using Flow Cytometry Data, Ming-Chih Shih, Shou-Hsuan Stephen Huang, Rachel Donohue, Chung-Che Chang, Youli Zu Jan 2013

Automatic B Cell Lymphoma Detection Using Flow Cytometry Data, Ming-Chih Shih, Shou-Hsuan Stephen Huang, Rachel Donohue, Chung-Che Chang, Youli Zu

Faculty Bibliography 2010s

Background: Flow cytometry has been widely used for the diagnosis of various hematopoietic diseases. Although there have been advances in the number of biomarkers that can be analyzed simultaneously and technologies that enable fast performance, the diagnostic data are still interpreted by a manual gating strategy. The process is labor-intensive, time-consuming, and subject to human error. Results: We used 80 sets of flow cytometry data from 44 healthy donors, 21 patients with chronic lymphocytic leukemia (CLL), and 15 patients with follicular lymphoma (FL). Approximately 15% of data from each group were used to build the profiles. Our approach was able …

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Global Stability Of Infectious Disease Models Using Lyapunov Functions, Zhisheng Shuai, P. Van Den Driessche Jan 2013

Global Stability Of Infectious Disease Models Using Lyapunov Functions, Zhisheng Shuai, P. Van Den Driessche

Faculty Bibliography 2010s

Two systematic methods are presented to guide the construction of Lyapunov functions for general infectious disease models and are thus applicable to establish their global dynamics. Specifically, a matrix-theoretic method using the Perron eigenvector is applied to prove the global stability of the disease-free equilibrium, while a graph-theoretic method based on Kirchhoff's matrix tree theorem and two new combinatorial identities are used to prove the global stability of the endemic equilibrium. Several disease models in the literature and two new cholera models are used to demonstrate the applications of these methods.

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Cideb Protein Is Required For The Biogenesis Of Very Low Density Lipoprotein (Vldl) Transport Vesicle, Samata Tiwari, Shaila Siddiqi, Shadab A. Siddiqi Jan 2013

Cideb Protein Is Required For The Biogenesis Of Very Low Density Lipoprotein (Vldl) Transport Vesicle, Samata Tiwari, Shaila Siddiqi, Shadab A. Siddiqi

Faculty Bibliography 2010s

Nascent very low density lipoprotein (VLDL) exits the endoplasmic reticulum (ER) in a specialized ER-derived vesicle, the VLDL transport vesicle (VTV). Similar to protein transport vesicles (PTVs), VTVs require coat complex II (COPII) proteins for their biogenesis from the ER membranes. Because the size of the VTV is large, we hypothesized that protein(s) in addition to COPII components might be required for VTV biogenesis. Our proteomic analysis, supported by Western blotting data, shows that a 26-kDa protein, CideB, is present in the VTV but not in other ER-derived vesicles such as PTV and pre-chylomicron transport vesicle. Western blotting and immunoelectron …

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The Role Of Habitat In The Persistence Of Fire Ant Populations, Walter R. Tschinkel, Joshua R. King Jan 2013

The Role Of Habitat In The Persistence Of Fire Ant Populations, Walter R. Tschinkel, Joshua R. King

Faculty Bibliography 2010s

The association of the exotic fire ant, Solenopsis invicta with man-modified habitats has been amply demonstrated, but the fate of such populations if ecological succession proceeds has rarely been investigated. Resurvey of a fire ant population in a longleaf pine plantation after 25 years showed that the recovery of the site from habitat disturbance was associated with a large fire ant population decline. Most of the persisting colonies were associated with the disturbance caused by vehicle tracks. In a second study, mature monogyne fire ant colonies that had been planted in experimental plots in native groundcover of the north Florida …

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Expression Of Fungal Cutinase And Swollenin In Tobacco Chloroplasts Reveals Novel Enzyme Functions And/Or Substrates, Dheeraj Verma, Shuangxia Jin, Anderson Kanagaraj, Nameirakpam D. Singh, Jaiyanth Daniel, Pappachan E. Kolattukudy, Michael Miller, Henry Daniell Jan 2013

Expression Of Fungal Cutinase And Swollenin In Tobacco Chloroplasts Reveals Novel Enzyme Functions And/Or Substrates, Dheeraj Verma, Shuangxia Jin, Anderson Kanagaraj, Nameirakpam D. Singh, Jaiyanth Daniel, Pappachan E. Kolattukudy, Michael Miller, Henry Daniell

Faculty Bibliography 2010s

In order to produce low-cost biomass hydrolyzing enzymes, transplastomic lines were generated that expressed cutinase or swollenin within chloroplasts. While swollenin expressing plants were homoplasmic, cutinase transplastomic lines remained heteroplasmic. Both transplastomic lines showed interesting modifications in their phenotype, chloroplast structure, and functions. Ultrastructural analysis of chloroplasts from cutinase- and swollenin-expressing plants did not show typical lens shape and granal stacks. But, their thylakoid membranes showed unique scroll like structures and chloroplast envelope displayed protrusions, stretching into the cytoplasm. Unusual honeycomb structures typically observed in etioplasts were observed in mature chloroplasts expressing swollenin. Treatment of cotton fiber with chloroplast-derived swollenin …

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Discovering Transcription And Splicing Networks In Myelodysplastic Syndromes, Hongyan Wang, Jianguo Wen, Chung-Che Chang, Xiaobo Zhou Jan 2013

Discovering Transcription And Splicing Networks In Myelodysplastic Syndromes, Hongyan Wang, Jianguo Wen, Chung-Che Chang, Xiaobo Zhou

Faculty Bibliography 2010s

More and more transcription factors and their motifs have been reported and linked to specific gene expression levels. However, focusing only on transcription is not sufficient for mechanism research. Most genes, especially in eukaryotes, are alternatively spliced to different isoforms. Some of these isoforms increase the biodiversity of proteins. From this viewpoint, transcription and splicing are two of important mechanisms to modulate expression levels of isoforms. To integrate these two kinds of regulation, we built a linear regression model to select a subset of transcription factors and splicing factors for each co-expressed isoforms using least-angle regression approach. Then, we applied …

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Inhibited Growth Of Pseudomonas Aeruginosa By Dextran- And Polyacrylic Acid-Coated Ceria Nanoparticles, Qi Wang, J. Manuel Perez, Thomas J. Webster Jan 2013

Inhibited Growth Of Pseudomonas Aeruginosa By Dextran- And Polyacrylic Acid-Coated Ceria Nanoparticles, Qi Wang, J. Manuel Perez, Thomas J. Webster

Faculty Bibliography 2010s

Ceria (CeO2) nanoparticles have been widely studied for numerous applications, but only a few recent studies have investigated their potential applications in medicine. Moreover, there have been almost no studies focusing on their possible antibacterial properties, despite the fact that such nanoparticles may reduce reactive oxygen species. In this study, we coated CeO2 nanoparticles with dextran or polyacrylic acid (PAA) because of their enhanced biocompatibility properties, minimized toxicity, and reduced clearance by the immune system. For the first time, the coated CeO2 nanoparticles were tested in bacterial assays involving Pseudomonas aeruginosa, one of the most significant bacteria responsible for infecting …

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Kruppel-Like Factor 8 Promotes Tumorigenic Mammary Stem Cell Induction By Targeting Mir-146a, Xianhui Wang, Heng Lu, Tianshu Li, Lin Yu, Gang Liu, Xu Peng, Jihe Zhao Jan 2013

Kruppel-Like Factor 8 Promotes Tumorigenic Mammary Stem Cell Induction By Targeting Mir-146a, Xianhui Wang, Heng Lu, Tianshu Li, Lin Yu, Gang Liu, Xu Peng, Jihe Zhao

Faculty Bibliography 2010s

The properties of stem cells can be induced during the epithelial to mesenchymal transition (EMT). The responsible molecular mechanisms, however, remain largely undefined. Here we report the identification of the microRNA-146a (miR-146a) as a common target of Kruppel-like factor 8 (KLF8) and TGF-beta, both of which are known EMT-inducers. Upon KLF8 overexpression or TGF-beta treatment, a significant portion of the MCF-10A cells gained stem cell traits as demonstrated by an increased expression of CD44(high)/CD24(low), activity of aldehyde dehydrogenase (ALDH), mammosphere formation and chemoresistance. Along with this change, the expression of miR-146a was highly upregulated in the cells. Importantly, we found …

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Uniform Alumina Microspheres From Temperature Induced Forming In A Microfluidic T-Junction, James Wilson, Jonathan D. Wehking, Ranganathan Kumar Jan 2013

Uniform Alumina Microspheres From Temperature Induced Forming In A Microfluidic T-Junction, James Wilson, Jonathan D. Wehking, Ranganathan Kumar

Faculty Bibliography 2010s

This Letter explores a method for the manufacture of solid microspheres of 30 mu m, from liquid droplets with nanosuspensions of 20 nm alumina in a microfluidic T-junction. These droplets are heated downstream where solidification takes place due to Temperature Induced Forming (TIF). TIF occurs as temperature sensitive alumina solubility leads to weakening electrostatic double-layer dispersing forces resulting in the formation of a solid structure. Manipulation of the flow characteristics and material properties of the continuous and dispersed fluids provides control of droplet production over a range of conditions and allows for a robust manufacturing facility capable of producing particles …

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Beta-Hydroxy-Beta-Methylbutyrate Free Acid Reduces Markers Of Exercise-Induced Muscle Damage And Improves Recovery In Resistance-Trained Men, Jacob M. Wilson, Ryan P. Lowery, Jordan M. Joy, Joe A. Walters, Shawn M. Baier, John C. Fuller Jr., Jeffrey R. Stout, Layne E. Norton, Eric M. Sikorski, Stephanie M. C. Wilson, Nevine M. Duncan, Nelo E. Zanchi, John Rathmacher Jan 2013

Beta-Hydroxy-Beta-Methylbutyrate Free Acid Reduces Markers Of Exercise-Induced Muscle Damage And Improves Recovery In Resistance-Trained Men, Jacob M. Wilson, Ryan P. Lowery, Jordan M. Joy, Joe A. Walters, Shawn M. Baier, John C. Fuller Jr., Jeffrey R. Stout, Layne E. Norton, Eric M. Sikorski, Stephanie M. C. Wilson, Nevine M. Duncan, Nelo E. Zanchi, John Rathmacher

Faculty Bibliography 2010s

The purpose of the present study was to determine the effects of short-term supplementation with the free acid form of beta-hydroxy-beta-methylbutyrate (HMB-FA) on indices of muscle damage, protein breakdown, recovery and hormone status following a high-volume resistance training session in trained athletes. A total of twenty resistance-trained males were recruited to participate in a high-volume resistance training session centred on full squats, bench presses and dead lifts. Subjects were randomly assigned to receive either 3 g/d of HMB-FA or a placebo. Immediately before the exercise session and 48 h post-exercise, serum creatine kinase (CK), urinary 3-methylhistadine (3-MH), testosterone, cortisol and …

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Catalytic Nanoceria Are Preferentially Retained In The Rat Retina And Are Not Cytotoxic After Intravitreal Injection, Lily L. Wong, Suzanne M. Hirst, Quentin N. Pye, Christopher M. Reilly, Sudipta Seal, James F. Mcginnis Jan 2013

Catalytic Nanoceria Are Preferentially Retained In The Rat Retina And Are Not Cytotoxic After Intravitreal Injection, Lily L. Wong, Suzanne M. Hirst, Quentin N. Pye, Christopher M. Reilly, Sudipta Seal, James F. Mcginnis

Faculty Bibliography 2010s

Cerium oxide nanoparticles (nanoceria) possess catalytic and regenerative radical scavenging activities. The ability of nanoceria to maintain cellular redox balance makes them ideal candidates for treatment of retinal diseases whose development is tightly associated with oxidative damage. We have demonstrated that our stable water-dispersed nanoceria delay photoreceptor cell degeneration in rodent models and prevent pathological retinal neovascularization in vldlr mutant mice. The objectives of the current study were to determine the temporal and spatial distributions of nanoceria after a single intravitreal injection, and to determine if nanoceria had any toxic effects in healthy rat retinas. Using inductively-coupled plasma mass spectrometry …

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A Compensatory Mutation Provides Resistance To Disparate Hiv Fusion Inhibitor Peptides And Enhances Membrane Fusion, Matthew P. Wood, Amy L. Cole, Piotr Ruchala, Alan J. Waring, Lisa C. Rohan, Preston Marx, Patrick M. Tarwater, Phalguni Gupta, Alexander M. Cole Jan 2013

A Compensatory Mutation Provides Resistance To Disparate Hiv Fusion Inhibitor Peptides And Enhances Membrane Fusion, Matthew P. Wood, Amy L. Cole, Piotr Ruchala, Alan J. Waring, Lisa C. Rohan, Preston Marx, Patrick M. Tarwater, Phalguni Gupta, Alexander M. Cole

Faculty Bibliography 2010s

Fusion inhibitors are a class of antiretroviral drugs used to prevent entry of HIV into host cells. Many of the fusion inhibitors being developed, including the drug enfuvirtide, are peptides designed to competitively inhibit the viral fusion protein gp41. With the emergence of drug resistance, there is an increased need for effective and unique alternatives within this class of antivirals. One such alternative is a class of cyclic, cationic, antimicrobial peptides known as theta-defensins, which are produced by many non-human primates and exhibit broad-spectrum antiviral and antibacterial activity. Currently, the theta-defensin analog RC-101 is being developed as a microbicide due …

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Regulation Of Pten/Akt Pathway Enhances Cardiomyogenesis And Attenuates Adverse Left Ventricular Remodeling Following Thymosin Beta 4 Overexpressing Embryonic Stem Cell Transplantation In The Infarcted Heart, Binbin Yan, Reetu D. Singla, Latifa S. Abdelli, Pawan K. Singal, Dinender K. Singla Jan 2013

Regulation Of Pten/Akt Pathway Enhances Cardiomyogenesis And Attenuates Adverse Left Ventricular Remodeling Following Thymosin Beta 4 Overexpressing Embryonic Stem Cell Transplantation In The Infarcted Heart, Binbin Yan, Reetu D. Singla, Latifa S. Abdelli, Pawan K. Singal, Dinender K. Singla

Faculty Bibliography 2010s

Thymosin beta 4 (T beta 4), a small G-actin sequestering peptide, mediates cell proliferation, migration, and angiogenesis. Whether embryonic stem (ES) cells, overexpressing T beta 4, readily differentiate into cardiac myocytes in vitro and in vivo and enhance cardioprotection following transplantation post myocardial infarction (MI) remains unknown. Accordingly, we established stable mouse ES cell lines, RFP-ESCs and T beta 4-ESCs, expressing RFP and an RFP-T beta 4 fusion protein, respectively. In vitro, the number of spontaneously beating embryoid bodies (EBs) was significantly increased in T beta 4-ESCs at day 9, 12 and 15, compared with RFP-ESCs. Enhanced expression of cardiac …

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Deep Vascular Imaging In Wounds By Two-Photon Fluorescence Microscopy, Ciceron O. Yanez, Alma R. Morales, Xiling Yue, Takeo Urakami, Masanobu Komatsu, Tero A. H. Jarvinen, Kevin D. Belfield Jan 2013

Deep Vascular Imaging In Wounds By Two-Photon Fluorescence Microscopy, Ciceron O. Yanez, Alma R. Morales, Xiling Yue, Takeo Urakami, Masanobu Komatsu, Tero A. H. Jarvinen, Kevin D. Belfield

Faculty Bibliography 2010s

Deep imaging within tissue (over 300 mu m) at micrometer resolution has become possible with the advent of two-photon fluorescence microscopy (2PFM). The advantages of 2PFM have been used to interrogate endogenous and exogenous fluorophores in the skin. Herein, we employed the integrin (cell-adhesion proteins expressed by invading angiogenic blood vessels) targeting characteristics of a two-photon absorbing fluorescent probe to image new vasculature and fibroblasts up to approximate to 1600 mu m within wound (neodermis)/granulation tissue in lesions made on the skin of mice. Reconstruction revealed three dimensional (3D) architecture of the vascular plexus forming at the regenerating wound tissue …

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An Analysis Of Generational Differences And Their Effects On Schools And Student Performance, Lisa Paniale Jan 2013

An Analysis Of Generational Differences And Their Effects On Schools And Student Performance, Lisa Paniale

Electronic Theses and Dissertations

This study examined the effects of generational differences on student achievement of students in Brevard Public Schools, Brevard County, Florida. The independent variable was the generational cohorts (Traditionalist, Baby Boomer, Generation X, and Millennials). The dependent variable was the factors of job satisfaction, organizational commitment, and work motivation. A second dependent variable was Value-Added Measure (VAM) scores calculated by the Department of Education for the state of Florida for each teacher of grades K-12. These VAM scores were derived from the Florida Comprehensive Assessment Test (FCAT) Reading and Math Developmental Scale scores to show a teacher’s effect on student achievement. …

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Habitat Use And Feeding Ecology Of Delphinids Inferred From Stable Isotopes And Fatty Acid Signatures, Nicole Browning Jan 2013

Habitat Use And Feeding Ecology Of Delphinids Inferred From Stable Isotopes And Fatty Acid Signatures, Nicole Browning

Electronic Theses and Dissertations

Prey availability, directly or indirectly, affects all aspects of a predator’s life history and is a primary factor influencing habitat selection and movements. This is especially true for delphinid species where it has been documented that the behaviors and movements of dolphins are strongly influenced by food availability. Unfortunately, the feeding ecology and habitat use patterns of many of these species are poorly understood. Many methodologies that have been employed to explore these facets of dolphin ecology have limitations and constraints or are logistically infeasible. Stable isotope and fatty acid signature analyses have been used extensively on a wide variety …

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Examining The Effect Of The Universal Design For Learning Expression Principle On Students With Learning Disabilities In Science, Lisa Finnegan Jan 2013

Examining The Effect Of The Universal Design For Learning Expression Principle On Students With Learning Disabilities In Science, Lisa Finnegan

Electronic Theses and Dissertations

The significance of students being able to express and demonstrate their knowledge and understanding in all content areas has always been important especially in the sciences. Students under the Next Generation Science Standards will be required to participate in science discourse through a variety of approaches. This study examined student engagement and student demonstration of content knowledge in inclusive science classrooms through a quasiexperimental research design which included four case study participants with a learning disability. The researcher also evaluated student content knowledge through the implementation of Universal Design for Learning-Expression (UDL-E) through a non-replicated control group design. Data were …

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A Mixed-Methods Approach To Examining The Memphis Crisis Intervention Team (Cit) Model: An Exploratory Study Of Program Effectiveness And Institutionalization Processes, Megan Magers Jan 2013

A Mixed-Methods Approach To Examining The Memphis Crisis Intervention Team (Cit) Model: An Exploratory Study Of Program Effectiveness And Institutionalization Processes, Megan Magers

Electronic Theses and Dissertations

The present study utilized a mixed-methods strategy to examine the effectiveness, diffusion, and institutionalization of the Memphis Crisis Intervention Team (CIT) model. To evaluate the effectiveness of the training component of the CIT model, a panel research design was employed in which a sample of 179 law enforcement officers and 100 correctional officers in nine Florida counties were surveyed on the first day of training (pretest), the last day of training (posttest), and one month following their completion of CIT training (follow-up). These surveys measured the extent to which CIT training achieved several officer-level objectives, including increased knowledge of mental …

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The Effect Of Face Threat Mitigation On Instructor Credibility And Student Motivation In The Absence Of Instructor Nonverbal Immediacy, Laura Trad Jan 2013

The Effect Of Face Threat Mitigation On Instructor Credibility And Student Motivation In The Absence Of Instructor Nonverbal Immediacy, Laura Trad

Electronic Theses and Dissertations

Many years of communication research have shown that an increase in immediacy has been a major factor that affects students‘ perceptions of instructor credibility which in turn affects, both students‘ cognitive and affective learning, student state motivation and a variety of other positive outcomes. However, in order for immediacy to be effective, instructor and student must be in the same location at the same time. With the recent push toward on-line classes, what can we find to act like immediacy in a text based format? This research suggests that face threat mitigation can be used in a text-based environment, to …

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Evaluation Of A Mind-Body Website By Women With Breast Cancer, Laura Beck Jan 2013

Evaluation Of A Mind-Body Website By Women With Breast Cancer, Laura Beck

Electronic Theses and Dissertations

Despite having access to volumes of information, women newly diagnosed with breast cancer report a moderate level of distress related to their diagnosis, treatment, life expectancy, threat to current roles, and life-changing surgery and treatment choices. Web sites designed to teach people strategies to reduce distress are readily available online. The online format may be useful and practical for women who can access the site at their convenience, learn the components of the interventions at their own pace, and practice the strategies in the comfort of their home. The purpose of this study was to evaluate an online Mind-Body web …

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