Digital Commons Network^™

What Is Appealing?: Sex And Racial Differences In Perceptions Of The Physical Attractiveness Of Women, Rachel Sewell

The Pegasus Review: UCF Undergraduate Research Journal

Beauty and physical appearance are considered to be of great importance in today's society. In the U.S., many strive for physical perfection, and much attention is focused on the way one looks. A beauty ideal has been created over time through the combination of several elements within American culture, and this ideal image attempts to define female beauty as fitting into a certain mold. Certain characteristics have been deemed the most attractive when it comes to female physical attractiveness, and a model of beauty has been presented by the media. This research focuses on whether everyone buys into that ideal. …

Understanding Older Women's Experience Of Being Diagnosed With Breast Cancer, Molly Wilhelm

The Pegasus Review: UCF Undergraduate Research Journal

This study describes the experience of being diagnosed with early stage breast cancer for women age 65 and older. This qualitative study used content analysis to analyze twenty interviews of older women with early stage breast cancer. A variety of positive and negative factors influence the experience of being diagnosed with breast cancer. These factors include: their initial reactions, interactions with their respective medical teams, family support, religion, education, and misinformation. Nurses can use the information from this study to help make diagnosis more positive by educating older women about breast cancer and treatment, encouraging them to develop an optimistic …

Early Adolescent Latinas And Non-Coital Sexual Behavior: Individual, Social, And Parental Variables, Ellita Williams

The Pegasus Review: UCF Undergraduate Research Journal

Unplanned pregnancies and sexually transmitted infections (STI) are increasing at a faster rate among young, low income Latinas as compared to other low income groups of girls. However, little is known about the non-coital behavior that early adolescent girls may engage in, which puts them at risk for initiation of intercourse. This study examines the frequency of a particular non-coital behavior, being touched below the waist on top of clothes, and investigates how girls who engage in this behavior differ from those who do not with respect to individual (biologic and cognitive), social (peer norms and acculturation), and parental (single …

The Backward Masking Red Light Effect And Schizotypy: The Influence Of Sex, Diana C. Hernandez, Andrea Y. Ranieri

The Pegasus Review: UCF Undergraduate Research Journal

Previous research has shown a unique effect of red light on visual processing related to both schizophrenia and positive schizotypy. The current study examines whether this effect is influenced by sex in a more broadly-defined schizotypy sample. A location backward masking (BM) task with three color background conditions (red, green, and gray) was administered to 34 undergraduate students (59% female) with a high score on the Schizotypal Personality Questionnaire (SPQ) and 39 students (51% female) with a low score. Results revealed that the group by color interaction was significant for the male participants, while it did not approach significance in …

Analysis Of The Repair Of Topoisomerase Ii Dna Damage, Eric Goldstein

The Pegasus Review: UCF Undergraduate Research Journal

A large number of anti-cancer chemotherapeutics target DNA topoisomerases. Etoposide is a specific topoisomerase II poison that causes reversible double strand DNA breaks. This project analyses the repair of DNA damage induced by etoposide, a common anti-cancer chemotherapeutic. Through the comparison of two known DNA repair pathways, anti-cancer chemotherapy may become more cytotoxic. Double strand DNA break repair is mediated by either non-homologous end joining (NHEJ) or homologous recombination (HR). NHEJ repairs through direct ligation of a double stranded break, whereas HR utilizes a homologous template to recover the wild type sequence. Reporter cassettes involving the expression of green fluorescent …

Etiology Of Community Acquired Clostridium Difficile-Associated Disease, Shannan Sherman, Dr. Pamela Ark

The Pegasus Review: UCF Undergraduate Research Journal

A review of literature related to community acquired Clostridium difficile -associated disease (CA-CDAD) was conducted. Nine relevant studies were identified using the Cumulative Index to Nursing and Allied Health (CINAHL) and MEDLINE-EBSCOhost databases. Clinical practice recommendations were obtained from the Centers for Disease Control and Prevention. The studies provided information about epidemiology of infection due to C. difficile in the community and interventions to reduce transmission. Multiple studies found underlying gastrointestinal disorders and use of cephalosporin antibiotics to be a risk factor. Another risk factor was administration of gastric acid suppressive drugs. No particular C. difficile strain was more likely …

Cold Temperature Effects On Byssal Thread Production By The Native Mussel Geukensia Demissa Versus The Non-Native Mussel Mytella Charruana, Sasha Brodsky

The Pegasus Review: UCF Undergraduate Research Journal

Mytella charruana is a Central/South American mussel that has been found as an introduced species along the southeastern Atlantic coast of the United States since 2004. Previous experiments have determined the lethal thermal minimum of M. charruana to be between 6-9° C. Continuous byssal thread production is essential for mussel survival as it is used for attachment to substrates in natural environments, but may decrease when environmental conditions deviate from their optimum. We sought to determine whether M. charruana exhibits a non-lethal, thermal response involving a reduction in byssal thread production. Mytella charruana and, for comparison, a native mussel species, …

Characterization Of Cytokeratin 8 In Cancer, Daniel S. Leventhal

The Pegasus Review: UCF Undergraduate Research Journal

A Cytokeratin 8 (K8)/Green Fluorescent Protein (GFP) fusion construct was created to better understand the behavior of K8 within cancer cells. This intermediate filament (IF) protein is a member of the cytoskeletal gene family, along with actin and tubulin. IFs are normally expressed in a tissue-specific and differentiation-dependent manner, in which their role is more supportive than essential to the cell. Such roles include rigidity of cellular shape, protein trafficking, cellular locomotion, and cell-signaling platforms. K8 mutation, over-expression, and aberrant post translational modifications have been observed in various carcinoma cell lines to be the cause of several phenotypes, including apoptosis …

Silence Of The Sexes: Gender Inversion In Jonathan Demme's The Silence Of The Lambs, Megan Evans

The Pegasus Review: UCF Undergraduate Research Journal

"Silence of the Sexes: Gender Inversion in Jonathan Demme's The Silence of the Lambs" focuses on the role gender plays in and between the characters of Clarice Starling, Buffalo Bill, and Dr. Hannibal Lecter in Jonathan Demme's film The Silence of the Lambs (1991). Specifically, the paper focuses on the struggle for Clarice to masculinize herself to be accepted in her career, and the struggle for Buffalo Bill to feminize himself in order to accept himself. The paper then discusses the inability for the one person to succeed while the other is still an obstacle in his or her …

Allelopathy Of Invasive Brazilian Pepper (Schinus Terebinthifolius) On Mobile Invertebrates From The Indian River Lagoon, Lori Konar, Tiffany Sheldon, Stephanie Garvis, Melinda Donnelly

The Pegasus Review: UCF Undergraduate Research Journal

Brazilian pepper Schinus terebinthifolius is a highly invasive plant species that can be found in many different ecosystems, including the edges of estuaries in south and central Florida. Brazilian pepper fruits contain chemicals that are toxic to native salt marsh plants. Previous researchers found that high densities of crushed Brazilian pepper fruits negatively impacted growth and final biomass of the black mangrove Avicennia germinans and the red mangrove Rhizophora mangle. Here, we investigated the impact of S. terebinthifoliuson the viability of mobile estuarine invertebrates commonly found in the Indian River Lagoon, as well as the brine shrimp, a …

Factors Associated With Eating Disorders In Women, Christina Knowles

The Pegasus Review: UCF Undergraduate Research Journal

Although various factors associated with eating disorders have been studied, no comprehensive source of research findings was identified in this review. The purpose of this study was to identify and synthesize research findings of factors associated with eating disorders in women published from 1992-2008. These findings may be useful to nurses, other professionals, families, and the public to facilitate the prevention, recognition, treatment, and rehabilitation of women with eating disorders.

The factors most closely associated with eating disorders identified through this review were depression, sexual abuse, substance abuse, anxiety disorders, early pubertal onset and the personality traits of perfectionism and …

Computational Analysis Of Broad Complex Zinc-Finger Transcription Factors, Barbara Mascareno-Shaw

The Pegasus Review: UCF Undergraduate Research Journal

Studies of transcription factors have provided important contributions toward understanding the cell morphology and developmental regulation of fruit flies based on sequence analysis of amino acid substitutions. In this report we describe the construction of four protein-DNA models based on two experimentally determined X-ray crystal structures (2DRP and 1A1H) using computational simulations of Z1, Z2, Z3, and Z4 zinc-finger DNA binding proteins. Once these models were constructed, 3 mutants were generated for the Z1/Z3 proteins. We evaluated the construction of the models and mutants against the templates using molecular mechanics (MM3) calculations in an attempt to determine the effect of …

Hanging By A Thread: Enhancing The Forensic Value Of Dyed Cotton Trace Evidence Through The Application Of Novel Techniques In Fiber Discrimination, Rachel M. Russo

The Pegasus Review: UCF Undergraduate Research Journal

This thesis examines the capability of current techniques in fiber classification such as UV-visible microspectrophotometry (MSP) (for dye in situ and/or extracted) to discriminate between fibers from sources known to be different. When these methods fail to adequately distinguish the fibers, novel alternative techniques'such as pulsed pumped laser-induced fluorescence spectroscopy (LIF) and liquid chromatography-mass spectrometry (LC-MS)'are utilized to provide definitive forensic evidence.

The FBI Dye Extraction Classification and Chromatography Schemes: Forensic Fiber Examination Guidelines provides the methodology used by the majority of crime labs across the United States (Fong, 1984). In the case of cotton fibers'the most frequently encountered fiber …

Interplay Of Molecular And Nanoscale Behaviors In Biological Soft Matter, Nicholas Ciaffone

Electronic Theses and Dissertations

The complexity of biological soft matter at the sub-micrometer level is fundamentally correlated to the functionalities at the larger scale. Reflecting the level of heterogeneities in the properties of systems remains challenging when probing small scales, due to the mismatch between the area surveyed with the tools offering nanoscale resolution, such as atomic force microscopy (AFM), and the scale of natural variations inherent to biology. Hence, to understand the physiological and mechanical alterations that occur within a single cell relative to a cell population, a multiscale approach is necessary. In this work we show that it is possible to observe …

Tunable Effect Of Metal Ions On Polyelectrolyte Mechanics, Angie Diaz

Electronic Theses and Dissertations

Polyelectrolyte based hydrogel fibers can mimic extracellular matrix and have applications such as drug delivery and tissue scaffolding. Metal ions play a critical role in hydrogel fiber stability via electrostatic interactions, but knowledge of how they modulate mechanical properties of individual polyelectrolyte polymers is lacking. In this study, electrospun polyacrylic acid with chitosan is used as a model system to evaluate ferric ion effect on nanofiber mechanics. Using dark field microscopy imaging and persistence length analysis, we demonstrate that ferric ions modulate the bending stiffness of nanofibers. Young's modulus of individual nanofibers is estimated at values of a few kilopascals, …

The Influence Of Family Communication Styles On Campus Experience In College-Aged Children, Madison Kjosa

Honors Undergraduate Theses

As an environment, the modern university setting is diverse and subject students to numerous challenges and opportunities that prepare them to enter careers, build families, and grow as an individual. Yet what a student experiences in college differs greatly and is shaped by internal and external factors in their environment, including campus participation, college self-efficacy, depression and stress. Prior research indicates the impact of family on how a student expresses each of these variables (Schmidtgall, King, Zarski & Cooper, 2000; Bradbury & Mather, 2009; Hannum & Dvorak, 2004; Kenny & Donaldson, 1991; Lopez et al, 2001; Shaver & Mikulincer, 2006). …

Boitawl: Soil, Lost And Left, Bishnupriya Chowdhuri

Electronic Theses and Dissertations

Boitawl ("Boi"- lack, devoid of, "Tawl"- bottom/ ground/ foundation), the word in one of the Bengali dialects refers to one without a ground beneath her feet. The thesis, a hybrid collection of prose and verse including narratives and graphic vignettes, flash, fabulist and short stories, prose poems and free verse imagines the inside worlds of such un-settled existences. In the process, the pieces connect migration, memory, childhood and lost towns with fractured humans caught in between - to reveal what lies under pillars of desires, the shapes of unsaid longings and recurrent images in their dreams.

An Exploration Into The Psychotic Symptoms Associated With Schizophrenia And Major Depressive Disorder, Kyndester I. Michael-Samaroo

Honors Undergraduate Theses

This research focuses on examining the neurological similarities between schizophrenia and major depressive disorder with psychotic features in order to compare the manifestations of psychosis in each disorder. Both disorders often involve symptoms of psychosis, although the overall disorders are very different from each other. The hypothesis for this research is that the neurological similarities between schizophrenia and major depressive disorder with psychotic features will provide researchers with the strategies needed to develop a treatment for psychotic symptoms. In order to test this hypothesis, five related studies were gathered for each disorder, and three studies were gathered for psychosis. These …

Systematic Review And Meta-Analysis: Tuberculosis, Tnfα Inhibitors, And Crohn's Disease, Brent L. Cao

Honors Undergraduate Theses

Inflammation is often a protective reaction against harmful foreign agents. However, in many disease conditions, the mechanisms behind the inflammatory response are poorly understood. Often times, the inflammation causes adverse effects, such as joint pain, abdominal pain, fever, fatigue, and loss of appetite. Thus, many treatments aim to inhibit the inflammatory response in order to control adverse symptoms. Such treatments include TNFα inhibitors. However, a major risk associated with drugs inhibiting tumor necrosis factor alpha (TNFα) is serious infection, including tuberculosis (TB).

Anti-TNFα therapy is used to treat patients with Crohn’s disease, for which the risk of tuberculosis may be …

Development Of Methods To Modulate Natural Killer Cells, Kari A. Shaver

Honors Undergraduate Theses

Natural Killer (NK) cell based immunotherapies have demonstrated success against malignancies and hematological cancers. However, tumors have developed mechanisms to evade detection by and suppress the immune system, commonly through altering the expression of cell-surface proteins. Overexpression of human leukocyte antigen-E (HLA-E), which binds to the inhibitory NKG2A on NK cells, protects malignant cells from lysis. Downregulating the NKG2A receptor on NK cells should release NK cell inhibition, but proves challenging as NK cells are difficult to transfect and no good methods currently exist. This project is designed to investigate the use of exosomes – small vesicles and natural carriers …

Targeted Delivery Of A Therapeutic Protein For The Treatment Of Alzheimer's Disease, Heather Holman

Honors Undergraduate Theses

Neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease are linked to mitochondrial dysfunction and the underexpression of TOM40, a protein with chaperone-like qualities that is responsible for transporting precursor proteins into the mitochondria. Overexpression of TOM40 is reported to partially restore mitochondrial dysfunction and decrease the accumulation of neurotoxic aggregates of α-synuclein. Our goal is to develop an effective method for delivery of TOM40 protein to the brain.

Previous studies have used lentiviruses to carry TOM40 into the hippocampus of α-synuclein transgenic mice. The disadvantage of lentiviral transfection is the random insertions of the target gene into the host …

Young Adult Development And Caregiving: Disposition And Involvement In Care, Nicholas James

Electronic Theses and Dissertations

Caregivers are a diverse group of individuals with a variety of backgrounds and roles; however, research has widely overlooked young adults in this population. This study investigated young adult caregiver circumstance, burden, and use of support services with the purpose of improving future caregiving research and interventions. Study 1 compared burden and circumstance among young, middle aged, and older adult caregivers (N = 285) in addition to developmental indicators among young adults caregiver and demographically matched non-caregiver peers (n = 225). Study 2 surveyed caregivers from each age group (N = 151) on barriers to care, treatment preferences, and interest …

Unearthing The Past And Present Of A Semi-Fossorial Lizard: Conservation Genetics, Phylogeography, And Taxonomy Of Plestiodon Egregius., Kathryn Mercier

Electronic Theses and Dissertations

Characterizing an organism's evolutionary history and population structure as well as understanding the forces shaping that divergence is crucial to conservation biology. A clear understanding of the patterns of diversity and divergence are imperative for the best management of the organism, while an awareness of what drives these patterns can lead to better predictions of how organisms will respond to future climate change. Historical climate changes and associated sea level change are among the main forces driving divergence in many species. To examine how effects of climate changes may have driven patterns of intraspecific divergence, I examined Mole Skinks, Plestiodon …

Probing The Effects Of Substrate Stiffness On Astrocytes Mechanics, Ariege Bizanti

Electronic Theses and Dissertations

Astrocytes are among the most functionally diverse population of cells in the central nervous system (CNS) as they are essential to many important neurological functions including maintaining brain homeostasis, regulating the blood brain barrier, and preventing build-up of toxic substances within the brain, for example. Astrocyte importance to brain physiology and pathology has inspired a host of studies focused on understanding astrocyte behavior primarily from a biological and chemical perspective. However, a clear understanding of astrocyte dysfunction and their link to disease has been hampered by a lack of knowledge of astrocyte behavior from a biomechanical perspective. Furthermore, astrocytes (and …

Predicting Herbivore Induced Phytochemical Shifts In Helianthus Using Spectral Reflectance, Jessica Jowais

Honors Undergraduate Theses

Induced defense responses in plants vary greatly among species, with many species exhibiting strong upregulation of secondary metabolites under attack by herbivores or pathogens. Secondary metabolite responses are most commonly analyzed using nuclear magnetic resonance or mass spectroscopy, though such approaches are costly and time-intensive. This study explores the use of hyperspectral reflectance as a more time- and cost-efficient method of detecting herbivore-induced secondary metabolite responses in plants. A diverse cross-section of wild sunflowers (genus Helianthus) were grown under controlled conditions and challenged with insect herbivory. Hyperspectral reflectance data was collected and analyzed using a principal component analysis in …

The Effects Of Site-Directed Mutagenesis On Hemerythrin-Like Protein Rv2633c, Kelly M. Rosch

Honors Undergraduate Theses

Mycobacterium tuberculosis (Mtb) is the causative agent of tuberculosis, one of the top ten causes of death worldwide. One of the genes upregulated in Mtb during macrophage infection is rv2633c, but the structure and function of its gene product remain unknown. Preliminary research has indicated that Rv2633c is a hemerythrin-like protein that exhibits catalase activity and binds two iron atoms using an HHE domain. Additionally, Rv2633c appears to exist as a dimer. The purpose of this project is to identify specific residues outside of the HHE domain that contribute to the protein's iron-binding ability and/or catalase activity, …

Infection Dynamics Of Herpesvirus In Gopher Tortoises, Joanne Saldanha

Honors Undergraduate Theses

Gopherus polyphemus, commonly known as the Gopher Tortoise, is a dryland reptile native to the southeastern United States. It is commonly a resident of longleaf pine and dry oak sand hill habitats. It is considered a keystone species because they dig deep burrows that provide shelter to them as well as many other animals. Habitat loss, fragmentation, and disease are major threats and have caused this species to be federally listed as a threatened species under the Endangered Species Act (ESA). Disease is a major threat to the gopher tortoise’s survival, and with declining populations, the need to investigate …

Parasympathetic Nerve Derived Exosomes Inhibit Hyperglycemia Induced Apoptosis In Cardiomyoblast Cells, Reetish K. Singla

Honors Undergraduate Theses

Diabetic cardiomyopathy involves both forms of cardiac cell cell death such as apoptosis and necrosis. However, this remains unknown whether hyperglycemia induced apoptosis in the cell culture system is inhibited by parasympathetic nerve derived exosomes. We isolated parasympathetic and sympathetic nerves and derived exosomes. We developed hyperglycemia induced apoptosis in H9c2 cells. H9c2 cells were divided into 4 groups: 1) Control, 2) H9c2+ Glucose 100 mmol, 3) H9c2+ Glucose +parasympathetic-exo, 4) H9c2+ Glucose+sympathetic-exo. We determined cell proliferation and viability with MTT assay kit and apoptosis with TUNEL staining and cell death detection ELISA kit. Data was further confirmed with pro-apoptotic …

Mobility And Collapse: Stable Isotope Analysis Of Oxygen-18 Isotopes From Ancient Mexico, Melanie L. St. Pierre

Honors Undergraduate Theses

When a society experiences a collapse, political authority becomes decentralized, large settlements often become abandoned, economic specialization decreases; and monumental building projects, artistic, and literary achievements slow drastically. The Rio Verde Valley, a coastal floodplain located in the region of Oaxaca in Southwest Mexico, experienced such a collapse at the end of the Terminal Formative period (150 BC to 250 AD). A period of decentralization followed, with regional centers becoming the main seats of authority throughout the region. My aim is to understand how this collapse affected residential population mobility in the lower Rio Verde Valley between the pre-collapse Terminal …

Memory Of Words: A Categorization Task, Paulina Maxim

Honors Undergraduate Theses

Through the years, the Deese-Roediger-McDermott Paradigm has demonstrated to be a useful method of observing false memories from semantically related word lists. The present study was conducted fully online and measured memory performance dependent on categorization of words by using groups, as well as dragging words across the page as a form of interaction. In a 2 (Categorized, Non-Categorized) x 2 (Interactive, Non-Interactive) between-subject factorial experiment, 56 undergraduate students were shown 18 different lists of 15 associative words to be studied, one list at a time. Participants were given a free recall test immediately after studying each individual list. Participants …