Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

University of Central Florida

Discipline
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 1921 - 1950 of 7697

Full-Text Articles in Entire DC Network

The Effects Of Regulatory Orientation On Subjective Task Values, Ability Beliefs, And Gameplay In A Grammar Editing Computer Game, Stephen Strnad Jan 2018

The Effects Of Regulatory Orientation On Subjective Task Values, Ability Beliefs, And Gameplay In A Grammar Editing Computer Game, Stephen Strnad

Electronic Theses and Dissertations

This study investigated mean group differences in composite subjective task values, ability beliefs, and gameplay behaviors between low promotion and high promotion English as a Second Language (ESL) postsecondary students while playing two versions of a grammar-editing computer game. First, students were categorized according to their scores on the General Regulatory Focus Measure. Next, students played two identical versions of the grammar-editing game; in the second game version, an independent variable was added in the form of an in-game punishment. In the middle of each game version, students completed a modified version of the Expectancy-value Questionnaire. Independent samples t-tests were …

Go to article

Interpersonal Behavior Traits And Their Relationship To Administrator-To-Teacher Feedback: A Quantitative Study, Robert Walker Jan 2018

Interpersonal Behavior Traits And Their Relationship To Administrator-To-Teacher Feedback: A Quantitative Study, Robert Walker

Electronic Theses and Dissertations

The purpose of this study was to explore what, if any, relationship exists between the interpersonal behavior traits held by administrators and the quality of the feedback they provide to teachers. The Interpersonal Behavior Survey (IBS) was used to develop interpersonal behavior profiles for all the school-based administrators from a moderately sized school district who consented to participate in the study. Additionally, the comments submitted as feedback to teachers by the participating administrators were reviewed and scored using a rubric. Multiple regression analysis was performed to determine what, if any, relationship exists between the traits measured by the IBS and …

Go to article

Dynamically Tunable Plasmonic Structural Color, Daniel Franklin Jan 2018

Dynamically Tunable Plasmonic Structural Color, Daniel Franklin

Electronic Theses and Dissertations

Functional surfaces which can control light across the electromagnetic spectrum are highly desirable. With the aid of advanced modeling and fabrication techniques, researchers have demonstrated surfaces with near arbitrary tailoring of reflected/transmitted amplitude, phase and polarization - the applications for which are diverse as light itself. These systems often comprise of structured metals and dielectrics that, when combined, manifest resonances dependent on structural dimensions. This attribute provides a convenient and direct path to arbitrarily engineer the surface's optical characteristics across many electromagnetic regimes. But while many of these plasmonic systems struggle to compete with the efficiency of pre-existing technologies, the …

Go to article

Touching The Unreal: The Definition, Narrative Strategies, And Aesthetics Of 3d Cartoon Narratives., Nathan Snow Jan 2018

Touching The Unreal: The Definition, Narrative Strategies, And Aesthetics Of 3d Cartoon Narratives., Nathan Snow

Electronic Theses and Dissertations

"Touching the Unreal" follows the structure set out by Scott McCloud in Understanding Comics to argue that understanding cartoons is serious business and requires that we define the art form, outline its basic tenets, and theorize how the mind understands it. The dissertation argues for a new definition of 3D computer generated cartoons, beginning with the most basic definition applicable to all forms of animation and taking into account new technological developments before arriving at the 3D cartoon narratives of today. The dissertation outlines the basic facets of 3D cartoon narratives in terms of narrative and aesthetics, arguing that, in …

Go to article

Optical Properties Of Single Nanoparticles And Two-Dimensional Arrays Of Plasmonic Nanostructures, Yadong Zhou Jan 2018

Optical Properties Of Single Nanoparticles And Two-Dimensional Arrays Of Plasmonic Nanostructures, Yadong Zhou

Electronic Theses and Dissertations

The tunability of plasmonic properties of nanomaterials makes them promising in many applications such as molecular detection, spectroscopy techniques, solar energy materials, etc. In the thesis, we mainly focus on the interaction between light with single nanoparticles and two-dimensional plasmonic nanostructures using electrodynamic methods. The fundamental equations of electromagnetic theory: Maxwell's equations are revisited to solve the problems of light-matter interaction, particularly the interaction of light and noble nanomaterials, such as gold and silver. In Chapter 1, Stokes parameters that describe the polarization states of electromagnetic wave are presented. The scattering and absorption of a particle with an arbitrary shape …

Go to article

Development And Characterization Of Solid-Contact Paper-Based And Micro Ion-Selective Electrodes For Environmental Analysis, Stephanie Armas Jan 2018

Development And Characterization Of Solid-Contact Paper-Based And Micro Ion-Selective Electrodes For Environmental Analysis, Stephanie Armas

Electronic Theses and Dissertations

Ion-selective electrodes (ISEs) have extensively been used for food analysis, as medical diagnostic tools, and for some environmental applications. However, ISEs are hindered by the need of a bulky reference electrode and the pre-conditioning step of the sensor, which can often be lengthy. This work will discuss how the direct addition of target analyte into the cocktail sensing membrane can circumvent the pre-conditioning step. Furthermore, the work is presented in an optimized ready-to-use single strip design, where the bulky glass reference electrode (RE) is no longer needed. The bulky RE was replaced by drop casting a simple two-component mixture consisting …

Go to article

Advancements In Liquid Chromatography For The Determination Of Polycyclic Aromatic Compounds In Environmental Samples, Hugh Hayes Jan 2018

Advancements In Liquid Chromatography For The Determination Of Polycyclic Aromatic Compounds In Environmental Samples, Hugh Hayes

Electronic Theses and Dissertations

The purpose of this dissertation is to provide a new and improved analytical methodology for the analysis of polycyclic aromatic hydrocarbons (PAHs) and polycyclic aromatic sulfur heterocycles (PASHs) in environmental samples. Hundreds of PAHs and PASHs are present in the environment and have great toxicological importance and the chemical determination of these components is a priority. The complete normal-phase liquid chromatography (NPLC) retention behavior has been explored for these compounds. Retention indices were determined for 124 PAHs, 62 methyl-PAHs (MePAHs), 67 PASHs, and 80 alkyl-PASHs on an aminopropyl (NH2) stationary phase which represents the most comprehensive study of polycyclic aromatic …

Go to article

Design, Synthesis, Stability, And Photocatalytic Studies Of Sustainable Metal-Organic Frameworks, Matthew Logan Jan 2018

Design, Synthesis, Stability, And Photocatalytic Studies Of Sustainable Metal-Organic Frameworks, Matthew Logan

Electronic Theses and Dissertations

The presented dissertation focuses on the design, synthesis, and characterization of metal-organic frameworks (MOFs) composed of earth-abundant elements the exhibit photoredox activity and studied their application as heterogeneous photocatalysts in organic synthesis and in solar-to-chemical energy conversion. In particular, the structure-property relationships of titanium-based MOFs relating the structure of the organic building unit and the photophysical and photochemical activity of the solid material is studied. The first novel family of seven MOFs isoreticular to MIL-125-NH2, includes functionalized with N-alkyl groups with increasing chain length (methyl to heptyl) and with varying connectivity (primary or secondary). The functionalized materials displayed reduced optical …

Go to article

A Multiple Case Study Exploring The Relationship Between Engagement In Model-Eliciting Activities And Pre-Service Secondary Mathematics Teachers' Mathematical Knowledge For Teaching Algebra, Aline Abassian Jan 2018

A Multiple Case Study Exploring The Relationship Between Engagement In Model-Eliciting Activities And Pre-Service Secondary Mathematics Teachers' Mathematical Knowledge For Teaching Algebra, Aline Abassian

Electronic Theses and Dissertations

The goal of this research study was to explore the nature of the relationship between engagement in model-eliciting activities (MEAs) and pre-service secondary mathematics teachers' (PSMTs') mathematical knowledge for teaching (MKT) algebra. The data collection took place in an undergraduate mathematics education content course for secondary mathematics education majors. In this multiple case study, PSMTs were given a Learning Mathematics for Teaching (LMT) pre-assessment designed to measure their MKT algebra, and based on those results, three participants were selected with varying levels of knowledge. This was done to ensure varied cases were represented in order to be able to examine …

Go to article

A Study Of The Influence Of The Restorative Justice Model On Out-Of-School Suspensions In A Large School District, Brian Agard Jan 2018

A Study Of The Influence Of The Restorative Justice Model On Out-Of-School Suspensions In A Large School District, Brian Agard

Electronic Theses and Dissertations

The purpose of this quantitative study was to investigate whether the Restorative Justice model influenced the number of days that students were suspended out-of-school or the number of out-of-school suspension incidents. In addition, the researcher analyzed whether the Restorative Justice model had different impacts for the subgroups of students qualifying for free and reduced lunch (FRL), English Learners (EL), students qualifying for exceptional student education services (ESE), and three ethnic subgroups (White, Black, and Hispanic) in an urban school district in Central Florida for the sixth-, seventh-, and eighth-grade students for the seven school years from 2010-2011 to 2016-2017. Primary …

Go to article

Far-Infrared Bands In Plasmonic Metal-Insulator-Metal Absorbers Optimized For Long Wave Infrared, Rachel Evans Jan 2018

Far-Infrared Bands In Plasmonic Metal-Insulator-Metal Absorbers Optimized For Long Wave Infrared, Rachel Evans

Electronic Theses and Dissertations

Metal–insulator–metal (MIM) resonant absorbers comprise a conducting ground plane, a thin dielectric, and thin separated metal top-surface structures. Long-wave infrared (LWIR) fundamental absorptions are experimentally shown to be optimized for a ratio of dielectric thickness to top-structure dimension t/l > 0.08. The fundamental resonance wavelength is predicted by different analytic standing-wave theories to be ~2nl, where n is the dielectric refractive index. Thus, for the dielectrics SiO2, AlN, and TiO2, l values of a few microns give fundamentals in the 8-12 micron LWIR wavelength region. Agreement of observed fundamental resonance wavelength with theory is better for t/l > ~0.2. Harmonics at shorter …

Go to article

Impact Of Ionizing Radiation And Electron Injection On Carrier Transport Properties In Narrow And Wide Bandgap Semiconductors, Jonathan Lee Jan 2018

Impact Of Ionizing Radiation And Electron Injection On Carrier Transport Properties In Narrow And Wide Bandgap Semiconductors, Jonathan Lee

Electronic Theses and Dissertations

This study investigated the minority carrier properties of wide and narrow bandgap semiconductors. Included specifically are wide bandgap materials GaN and β-Ga2O3, and narrow bandgap InAs/GaSb type-II strain-layer superlattice. The importance of minority carrier behavior in bipolar device performance is utmost because it is the limiting component in current conduction. The techniques used to determine minority carrier properties include electron beam induced current (EBIC) and cathodoluminescence (CL) spectroscopy. The CL spectroscopy is complemented with time-resolved CL (TRCL) for direct measurement of carrier radiative recombination lifetime. The minority carrier properties and effect of high energy radiation is explored. The GaN TRCL …

Go to article

A Quasi-Experiment On The Degree To Which I-Ready Reading Instruction Predicted Florida State Assessment Scores For Low Performing Students Compared To Students On Grade Level, Ashley Pierce Jan 2018

A Quasi-Experiment On The Degree To Which I-Ready Reading Instruction Predicted Florida State Assessment Scores For Low Performing Students Compared To Students On Grade Level, Ashley Pierce

Electronic Theses and Dissertations

The purpose of this study was to determine the degree to which i-Ready® Reading Instruction (a computer adaptive testing program) predicted Florida Standards Assessment English Language Arts (FSA ELA) scores for low performing students (Level 1) compared to those who are on grade level (Level 3). Participants included students in seventh grade at a central Florida school district who participated in i-Ready® Reading Instruction and who had previously scored a Level 1 or Level 3 on the FSA ELA. A hierarchical multiple regression was run to determine the impact of the interaction effect between prior year FSA performance level and …

Go to article

A Causal Comparative Analysis Of A Computer Adaptive Mathematics Program Using Multilevel Propensity Score Matching, Justin Seabolt Jan 2018

A Causal Comparative Analysis Of A Computer Adaptive Mathematics Program Using Multilevel Propensity Score Matching, Justin Seabolt

Electronic Theses and Dissertations

The purpose of this study was to examine the effectiveness of a computer adaptive mathematics program, i-Ready® Mathematics Instruction in Central Florida School District (CFSD) using a variety of quantitative analyses. In particular, students in the fifth grade at CFSD were examined in relation to the gains made on their end-of-year statewide mathematics assessment, known as the Florida Standards Assessment (FSA Math). Per i-Ready®, students who use i-Ready® Mathematics Instruction for a minimum of 45 minutes per subject per week for at least 25 weeks show significantly greater gains than those who do not (Curriculum Associates, 2017b). Therefore, two propensity …

Go to article

Design And Production Of Calculus Assessments, Lorna Wenzel Jan 2018

Design And Production Of Calculus Assessments, Lorna Wenzel

Electronic Theses and Dissertations

The AP Calculus program at Green High School was not adequately preparing students for college level calculus as more than 80 percent of the students failed the AP exam. The students were concurrently receiving high marks on in-class assessments. Hence, the in-class assessments were not good indicators of students calculus understanding according to the AP standards. A needs assessment was conducted, focusing on the difference in high school and college calculus, how to assess calculus and where Green High School's assessments were falling short. More research was conducted to examine the college Board expectations of calculus learning. A content matrix …

Go to article

International Saudi Female Students' Experiences Of Acculturation During Their Study At An American Public University In The South (Apus), Hnan Alzahrani Jan 2018

International Saudi Female Students' Experiences Of Acculturation During Their Study At An American Public University In The South (Apus), Hnan Alzahrani

Electronic Theses and Dissertations

According to my literature review and my reflection of its meaning, few studies have been conducted that explore the adjustment and acculturation experiences of female international students in US colleges and universities specifically on Saudi female students. Therefore, this study explored the academic and socio-cultural experiences of Saudi female students before and after arriving in the US. Furthermore, this study identified the sources of acculturative stress and Saudi female students' successful strategies for coping with these stressors. Eight Saudi female students participated in one-on-one semi-structured interviews and Moustakas' (1994) transcendental phenomenology was utilized in analyzing students' voices. During the data …

Go to article

Qualitative And Quantitative Elemental Composition Analysis Of The Surrounding Pollen Coating Via Icp-Ms, Brandy Voyer Jan 2018

Qualitative And Quantitative Elemental Composition Analysis Of The Surrounding Pollen Coating Via Icp-Ms, Brandy Voyer

Electronic Theses and Dissertations

Pollen evidence has proven to be a powerful forensic tool to trace a suspect or item to a victim or a crime scene. This is possible because it is microscopic, abundant in nature, resistant to degradation and decay; it presents dispersal patterns that can be used to generate a 'fingerprint' within specific areas, and has illustrated a unique morphology that can be used to classify species. While the pollen grain morphology has been extensively used to characterize the specific species, not much has been investigated as pertains to the coating that surrounds the pollen grain aside from it being categorized …

Go to article

Quasi-Gorenstein Modules, Alexander York Jan 2018

Quasi-Gorenstein Modules, Alexander York

Electronic Theses and Dissertations

This thesis will study the various roles that quasi-Gorenstein modules and their properties play in the study of homological dimensions and linkage of modules. To that effect we begin by studying these modules in their own right. An R-module M of grade g will be quasi-Gorenstein if ExtiR(M, R) = 0 for i 6= g and there is an isomorphism M ∼= ExtgR(M, R). Such modules have many nice properties which we will explore throughout this thesis. We will show they help extend a characterization of diagonalizable matrices over principal ideal domains to more general rings. We will use their …

Go to article

Mode-Locked Laser Based On Large Core Yb3+-Doped Fiber, Fei Jia Jan 2018

Mode-Locked Laser Based On Large Core Yb3+-Doped Fiber, Fei Jia

Electronic Theses and Dissertations

The thesis reviews principle of laser cavity and gives a general introduction to modelocked laser (MLL). By using Yb3+-doped fiber as gain medium, passive MLL cavity is developed in experiment, aiming to obtain femtosecond pulses with high pump power from 25W to 35W. The gain medium fiber with 65µm core diameter is cleaved with one flat end and another angled. Pump laser with 976nm wavelength is coupled into Yb3+ -doped fiber to excite signal from 1020nm to 1040nm in the core. 9W is threshold for laser setup. After locking all modes, picosecond pulses are output from laser cavity and coupled …

Go to article

Analysis And Design Of Non-Hermitian Optical Systems, Ali Kazemi Jahromi Jan 2018

Analysis And Design Of Non-Hermitian Optical Systems, Ali Kazemi Jahromi

Electronic Theses and Dissertations

From a very general perspective, optical devices can be viewed as constructions based on the spatial engineering of the optical index of refraction. Sculpting the real part of the refractive index produces the wide variety of known passive optical devices, such as waveguides, resonators, gratings, among a plethora of other possibilities for managing the transport of light. Less attention has been directed to engineering the imaginary part of the refractive index – that is responsible for optical gain and absorption – in conjunction with the real part of the refractive index. Optical gain is the building block of amplifiers and …

Go to article

An Examination Of Beta Diversity Indices And Their Predictors In Two Large-Scale Systems, Philip Schroeder Jan 2018

An Examination Of Beta Diversity Indices And Their Predictors In Two Large-Scale Systems, Philip Schroeder

Electronic Theses and Dissertations

Biodiversity is what conservation biology was developed to conserve. It is the physical manifestation of life as a concept and, be it for practical or idealistic reasons, all conservationists seek to protect or, in some cases, enhance it. Because of its monolithic importance to the field, much effort has been expended trying to better measure and understand it. Recently, greater attention has been paid to the partition of diversity; the observation that the total diversity of a system (γ) can be broken down into within-site diversity (α) and between-site diversity (β). In particular, it has been noticed that the β …

Go to article

An Analysis Of Choice-Making As A Means To Decrease The Frequency Of Self-Injurious Behaviors In Students With Severe Disabilities, Melanie Olson Jan 2018

An Analysis Of Choice-Making As A Means To Decrease The Frequency Of Self-Injurious Behaviors In Students With Severe Disabilities, Melanie Olson

Electronic Theses and Dissertations

This single case multiple baseline research study examined choice-making as a means to decrease the frequency of self-injurious behaviors in six students with severe disabilities. Five males and one female between the ages of 14 and 21 participated in the five-week intervention. The following research questions were addressed: 1) Does the choice-making intervention reduce hitting, biting, and self-injurious behaviors? 2) How much time does the choice-making intervention add to the classroom teacher's preparation? 3) What costs are associated with the choice-making intervention during an average lesson? The choice-making intervention was associated with positive behavioral outcomes for all of the students. …

Go to article

Nonlinear Dynamics In Multimode Optical Fibers, Mohammad Amin Eftekhar Jan 2018

Nonlinear Dynamics In Multimode Optical Fibers, Mohammad Amin Eftekhar

Electronic Theses and Dissertations

Multimode optical fibers have recently reemerged as a viable platform for addressing a number of long-standing issues associated with information bandwidth requirements and power-handling capabilities. The complex nature of heavily multimoded systems can be effectively exploited to observe altogether novel physical effects arising from spatiotemporal and intermodal linear and nonlinear processes. Here, we have studied nonlinear dynamics in multimode optical fibers (MMFs) in both the normal and anomalous dispersion regimes. In the anomalous dispersion regime, the nonlinearity leads to a formation of spatiotemporal 3-D solitons. Unlike in single-mode fibers, these solitons are not unique and their properties can be modified …

Go to article

An Examination Of The Algebra 1 Achievement Of Black And Hispanic Student Participants In A Large Urban School District's Mathematics Intervention Program, Elethia Bronson Jan 2018

An Examination Of The Algebra 1 Achievement Of Black And Hispanic Student Participants In A Large Urban School District's Mathematics Intervention Program, Elethia Bronson

Electronic Theses and Dissertations

The mathematics achievement gap between Black and White as well as Hispanic and White students has been well documented nationwide and in the school district of study. Much has been written in observance of the achievement gap, yet markedly less research has focused on practices and interventions that have improved mathematics performance for Black and Hispanic students. Consequently, this study examined the Algebra 1 achievement (indicated by student scale scores on the Florida Standards Assessments Algebra 1 End-of-Course exam) of Black and Hispanic students participating in a mathematics intervention program as compared to the Algebra 1 achievement of their similar …

Go to article

Weierstrass Vertices And Divisor Theory Of Graphs, Ajani Ruwandhika Chulangi De Vas Gunasekara Jan 2018

Weierstrass Vertices And Divisor Theory Of Graphs, Ajani Ruwandhika Chulangi De Vas Gunasekara

Electronic Theses and Dissertations

Chip-firing games and divisor theory on finite, connected, undirected and unweighted graphs have been studied as analogs of divisor theory on Riemann Surfaces. As part of this theory, a version of the one-dimensional Riemann-Roch theorem was introduced for graphs by Matt Baker in 2007. Properties of algebraic curves that have been studied can be applied to study graphs by means of the divisor theory of graphs. In this research, we investigate the property of a vertex of a graph having the Weierstrass property in analogy to the theory of Weierstrass points on algebraic curves. The weight of the Weierstrass vertices …

Go to article

Single Mode Wavelength-Tunable Thulium Fiber, Dong Jin Shin Jan 2018

Single Mode Wavelength-Tunable Thulium Fiber, Dong Jin Shin

Electronic Theses and Dissertations

Thulium fiber lasers have the broadest emission wavelength bandwidth out of any rare-earth doped fiber lasers. The emission wavelength starts from 1.75μm and ends at around 2.15μm, covering a vast swath of the eye safe wavelength region and intersecting with a large portion of mid-infrared atmospheric transmission window. Also, thulium fiber lasers provide the highest average output power of any other rare-earth doped fiber lasers in these wavelength regimes, making them uniquely suited for applications such as remote sensing. At the moment, high power beam propagation of continuous wave laser through the atmosphere in the mid-infrared range is yet to …

Go to article

2 Micron Fiber Lasers: Power Scaling Concepts And Limitations, Alex Sincore Jan 2018

2 Micron Fiber Lasers: Power Scaling Concepts And Limitations, Alex Sincore

Electronic Theses and Dissertations

Thulium- and holmium-doped fiber lasers (TDF and HDF) emitting at 2 micron offer unique benefits and applications compared to common ytterbium-doped 1 micron lasers. This dissertation details the concepts, limitations, design, and performance of four 2 micron fiber laser systems. While these lasers were developed for various end-uses, they also provide further insight into two major power scaling limitations. The first limitation is optical nonlinearities: specifically stimulated Brillouin scattering (SBS) and modulation instability (MI). The second limitation is thermal failure due to inefficient pump conversion. First, a 21.5 W single-frequency, single-mode laser with adjustable output from continuous-wave to nanosecond pulses …

Go to article

Integration Of Fundamental Research And Cer: The Role Of Authenticity In Developing Views On The Nature Of Teaching, Learning, And Doing Science, Julie Donnelly Jan 2018

Integration Of Fundamental Research And Cer: The Role Of Authenticity In Developing Views On The Nature Of Teaching, Learning, And Doing Science, Julie Donnelly

Electronic Theses and Dissertations

This dissertation is an integration of fundamental research and chemical education. It begins with two nonlinear spectroscopic studies of compounds important to the study of brain chemistry. In Chapter 2, we present a novel method using quantum mechanics for modelling ligand docking and the potential of nonlinear circular dichroism for elucidating the mechanism of cannabinoids docking to their receptor, a contribution to studies of varying psychological effects of cannabinoids. Considering existent challenges with measuring this phenomenon, in Chapter 3, we evaluate two-photon absorption properties of Thioflavin T (ThT) in varying glycerol/water content solutions and discuss the enhancement of nonlinear absorption …

Go to article

Nanoscale Characterization And Mechanism Of Electroless Deposition Of Silver Metal, Christopher Grabill Jan 2018

Nanoscale Characterization And Mechanism Of Electroless Deposition Of Silver Metal, Christopher Grabill

Electronic Theses and Dissertations

This dissertation is an investigation of the nanoscale characteristics and mechanism of electrolessly deposited silver metal seeded by gold nanoparticles. The process of growing seed-nanoparticles on a polymer surface was studied. Several bifunctional amines and organic reducing agents were used to explore how these chemical factors affect the size and distribution of gold nanoparticles formed at the interface. The nanoparticles were characterized by transmission electron microscopy (TEM) and high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM). An electroless deposition (ED) bath developed by Danscher was selected to study electroless deposition of silver in detail. The chemical species in the bath …

Go to article

Split Aptameric Turn-On Fluorescence Sensor For Detection Of Sequence Specific Nucleic Acid At Ambient Temperature, Nanami Kikuchi Jan 2018

Split Aptameric Turn-On Fluorescence Sensor For Detection Of Sequence Specific Nucleic Acid At Ambient Temperature, Nanami Kikuchi

Electronic Theses and Dissertations

Nucleic acid amplification tests (NAATs) enable sensitive detection of low density infections that microscopy and rapid diagnostic test are not capable of detecting. They enable quantitative and qualitative nucleic acid detection, genotype analysis, and single nucleotide polymorphism (SNP) detection. Current state of the art molecular probes used with NAATs includes molecular beacon (MB), Taqman and its variations. This work presents novel molecular probe designed from Spinach and Dapoxyl aptamers. The aptamers are split into two parts (split aptamer), allowing greater sensitivity and selectivity towards fully complementary nucleic acid analyte. The major advantage of split aptamer probe over state-of-the-art fluorescent probes …

Go to article

First Prev Next Last