Digital Commons Network^™

Fibergramme Vol.27 No. 2, Weavers O. Orlando

Text Materials of Central Florida

FIBERGRAMME is published in September, November, January, March and May.

Sandspur, Vol 109, No 13, February 07, 2003, Rollins College

The Rollins Sandspur

Rollins College student newspaper, written by the students and published at Rollins College. The Sandspur started as a literary journal.

Central Florida Future, Vol. 35 No. 40, February 3, 2003

Central Florida Future

LOST 16 minutes from home: Space shuttle Columbia disintegrates over Texas 7 astronauts perish.

Sandspur, Vol 109, No 12, January 31, 2003, Rollins College

The Rollins Sandspur

Rollins College student newspaper, written by the students and published at Rollins College. The Sandspur started as a literary journal.

Central Florida Future, Vol. 35 No. 38, January 27, 2003

Central Florida Future

Greeks maintain upper hand in student politics; Simulating war; Inflammatory e-mail stuns student government.

Central Florida Future, Vol. 35 No. 33, January 9, 2003

Central Florida Future

Universities vie for politicians; Gay rights laws take shape in city Will UCF be next?; Web site aims to curtail student drinking.

Vol Xii (2003)

The Marjorie Kinnan Rawlings Journal of Florida Literature

No abstract provided.

"This Ain't No Slavery Time Talk": The Evolution Of African American Folklore In Hurston's "Go Gator And Muddy The Water", L. Adam Mekler

The Marjorie Kinnan Rawlings Journal of Florida Literature

No abstract provided.

A Study Of High School Students' Perceptions Of Their Safety In School, Christina L. Testani Cafiero

Retrospective Theses and Dissertations

This study was designed to investigate the factors that contributed to high school students' sense of security at school, and what these students perceived as measures that if put in place, would increase their feelings of security. The focus was the relationship between students' perceptions of safety at school and their own opinions of school and their experiences at school.

The study was conducted in the School District of Osceola County, Florida. The population of this study was comprised of all students enrolled in the public high schools in the School District of Osceola County. The sample for the study …

8th Annual Joseph C. Andrews Mentoring Breakfast, March 13, 2003, Black Faculty And Staff Association

BFSA Collection - Mentoring Breakfast Materials

No abstract provided.

Fibergramme Vol. 27, No. 1, Weavers Of Orlando

Text Materials of Central Florida

FIBERGRAMME is published in September, November, January, March and May.

Sulfolipid Deficiency Does Not Affect The Virulence Of Mycobacterium Tuberculosis H37rv In Mice And Guinea Pigs, Cécile Rousseau, Oliver C. Turner, Erik Rush, Yann Bordat, Tatiana D. Sirakova, Pappachan E. Kolattukudy, Shannon Ritter, Ian M. Orme, Brigitte Gicquel, Mary Jackson

Faculty Bibliography 2000s

Lipids that are found only in the cell envelope of pathogenic mycobacteria, such as those containing multiple methyl-branched fatty acids, have long been thought to play a role in pathogenesis. Among these complex lipids, sulfolipids have been the most extensively studied over the last 50 years. The numerous biological effects exhibited by purified sulfolipids on phagocytic cells led to the idea that these molecules are probably important virulence factors facilitating the intracellular survival of Mycobacterium tuberculosis. However, definitive evidence to support this concept has been lacking. The recent construction of an isogenic sulfolipid-deficient mutant of M. tuberculosis H37Rv (Sirakova et …

Use Of Population Viability Analysis And Reserve Selection Algorithms In Regional Conservation Plans, Carlos Carroll, Reed F. Noss, Paul C. Paquet, Nathan H. Schumaker

Faculty Bibliography 2000s

Current reserve selection algorithms have difficulty evaluating connectivity and other factors necessary to conserve wide-ranging species in developing landscapes. Conversely, population viability analyses may incorporate detailed demographic data, but often lack sufficient spatial detail or are limited to too few taxa to be relevant to regional conservation plans. We developed a regional conservation plan for mammalian carnivores in the Rocky Mountain region using both a reserve selection algorithm (SITES) and, a spatially explicit population model (PATCH). The spatially explicit population model informed reserve selection and network design by producing data on the locations of population sources, the degree of threat …

Biochemical Function Of Msl5 (Pks8 Plus Pks17) In Mycobacterium Tuberculosis H37rv: Biosynthesis Of Monomethyl Branched Unsaturated Fatty Acids, Vinod S. Dubey, Tatiana D. Sirakova, Michael H. Cynamon, Pappachan E. Kolattukudy

Faculty Bibliography 2000s

We show that the disruption of one of the mycocerosic acid synthase (mas)-like genes, msl5 (pks8 plus pks17) in Mycobacterium tuberculosis H37Rv generates a mutant incapable of producing monomethyl branched unsaturated C-16 to C-20 fatty acids that are minor constituents of acyltrehaloses and sulfolipids. The msl5 mutation did not cause any significant change in the acyl lipid composition and also did not affect growth in culture, in mouse alveolar macrophage cell line MH-S, or in the murine lung.

Potential Involvement Of Extracellular Signal-Regulated Kinase 1 And 2 In Encystation Of A Primitive Eukaryote, Giardia Lamblia - Stage-Specific Activation And Intracellular Localization, John G. Ellis Iv, Monica Davila, Ratna Chakrabarti

Faculty Bibliography 2000s

Mitogen-activated protein kinase (MAPK) pathways are major signaling systems by which eukaryotic cells convert environmental cues to intracellular events such as proliferation and differentiation. We have identified Giardia lamblia homologues of two members of the MAPK family ERK1 and ERK2. Functional characterization of giardial ERK1 and ERK2 revealed that both kinases were expressed in trophozoites and encysting cells as 44- and 41-kDa polypeptides, respectively, and were catalytically active. Analysis of the kinetic parameters of the recombinant proteins showed that ERK2 is similar to5 times more efficient than ERK1 in phosphorylating myelin basic protein as a substrate, although the phosphorylating efficiency …

A Chloroplast Transgenic Approach To Hyper-Express And Purify Human Serum Albumin, A Protein Highly Susceptible To Proteolytic Degradation, Alicia Fernández-San Millán, Angel Mingo-Castel, Michael Miller, Henry Daniell

Faculty Bibliography 2000s

Human Serum Albumin (HSA) accounts for 60% of the total protein in blood serum and it is the most widely used intravenous protein in a number of human therapies. HSA, however, is currently extracted only from blood because of a lack of commercially feasible recombinant expression systems. HSA is highly susceptible to proteolytic degradation in recombinant systems and is expensive to purify Expression of HSA in transgenic chloroplasts using Shine-Dalgarno sequence (SD), which usually facilitates hyper-expression of transgenes, resulted only in 0.02% HSA in total protein (tp). Modification of HSA regulatory sequences using chloroplast untranslated regions (UTRs) resulted in hyper-expression …

Attenuation Of Mycobacterium Tuberculosis By Disruption Of A Mas-Like Gene Or A Chalcone Synthase-Like Gene, Which Causes Deficiency In Dimycocerosyl Phthiocerol Synthesis, Tatiana D. Sirakova, Vinod S. Dubey, Michael H. Cynamon, Pappachan E. Kolattukudy

Faculty Bibliography 2000s

Tuberculosis is one of the leading preventable causes of death. Emergence of drug-resistant tuberculosis makes the discovery of new targets for antimyrobacterial drugs critical. The unique mycobacterial cell wall lipids are known to play an important role in pathogenesis, and therefore the genes responsible for their biosynthesis offer potential new targets. To assess the possible role of some of the genes potentially involved in cell wall lipid synthesis, we disrupted a mas-like gene, msl7, and a chalcone synthase-like gene, pks10, with phage-mediated delivery of the disruption construct, in which the target gene was disrupted by replacement of an internal segment …

The Largest Open Reading Frame (Pks12) In The Mycobacterium Tuberculosis Genome Is Involved In Pathogenesis And Dimycocerosyl Phthiocerol Synthesis, Tatianna D. Sirakova, Vinod S. Dubey, Hwa-Jung Kim, Michael H. Cynamon, Pappachan E. Kolattukudy

Faculty Bibliography 2000s

The cell wall lipids in Mycobacterium tuberculosis are probably involved in pathogenesis. The largest open reading frame in the genome of M. tuberculosis H37Rv, pks12, is unique in that it encodes two sets of domains needed to produce fatty acids. Apks12-disrupted mutant was produced, and disruption was confirmed by both PCR analysis and Southern blotting. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that a 430-kDa protein band present in the wild type was missing in the mutant. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MS) and liquid chromatography (LC)-MS analysis of tryptic peptides showed that 54 peptides distributed …

Reckoning With Homelessness, James D. Wright

Faculty Bibliography 2000s

No abstract provided.

Developing Florida: No Cause For Celebration, Ellen Smith

The Marjorie Kinnan Rawlings Journal of Florida Literature

No abstract provided.

It's All In The Rhetoric: Using Affective Design To Change Users' Perceptions Of Online Help, Robert C. Stultz

Retrospective Theses and Dissertations

This thesis investigates the role of affective design in online help systems. The thesis begins with a discussion of the current research related to online help systems and the rhetorical nature of technical communication. The thesis then provides a heuristic evaluation of two versions of procedural discourse for Microsoft Word 2000. Based on an analysis of the heuristic evaluation, five recommendations are given for improving the affective design of online help systems. The thesis concludes with a proposed usability test and consideration of a related area of study.

University Of Central Florida Commencement, December 13 & 14, 2002, University Of Central Florida

Commencement Programs

No abstract provided.

Sandspur, Vol 109, No 10, December 06, 2002, Rollins College

The Rollins Sandspur

Rollins College student newspaper, written by the students and published at Rollins College. The Sandspur started as a literary journal.

Central Florida Future, Vol. 35 No. 31, December 5, 2002

Central Florida Future

Fraternity honors departed brother; Surviving the streets.

Central Florida Future, Vol. 35 No. 29, November 25, 2002

Central Florida Future

Students receive a taste of hunger: Banquet addresses real-world poverty; Hitt's raise pushes him to the top; Higher athletic fee to improve sports teams.

Central Florida Future, Vol. 35 No. 28, November 21, 2002

Central Florida Future

Extra fee could raise $1 million for athletics; Outlook for faculty union uncertain.

Central Florida Future, Vol. 35 No. 27, November 18, 2002

Central Florida Future

Professor sues university to keep position; Future uncertain for Florida's boards of trustees; $93,000 raise proposed for Hitt.

Central Florida Future, Vol. 35 No. 25, November 12, 2002

Central Florida Future

The check is not in the mail: With three weeks to go before finals some still await their financial aid; Making the grade the second time; Survey says: SGA needs to inform its constituents better.

Central Florida Future, Vol. 35 No. 24, November 7, 2002

Central Florida Future

Students helps dictate small class size smoking ban; Voter verdict: President's popularity caries party - and his brother.

Fibergramme, Weavers Of Orlando

Text Materials of Central Florida

FIBERGRAMME is published in September, November, January, March and May.