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Expression Of Heterologous Proteins In Transgenic Tobacco Chloroplasts To Produce A Biopharmaceutical And Biopolymer, Andrew Leon Devine Jan 2006

Expression Of Heterologous Proteins In Transgenic Tobacco Chloroplasts To Produce A Biopharmaceutical And Biopolymer, Andrew Leon Devine

Electronic Theses and Dissertations

The chloroplast has been demonstrated to be an ideal compartment to accumulate certain proteins or their biosynthetic products that would be harmful if they were accumulated in the cytoplasm. Hyper-expression of foreign proteins in chloroplast transgenics has accumulated up to 46% total soluble protein, this is possible due to the ~100 chloroplast genomes per chloroplast and ~100 chloroplasts per cell which can therefore, contain up to 10,000 copies of the transgene. Maternal gene inheritance of plastids in most crop plants results in natural gene containment. Chloroplast transformation also eliminates positional effects that are frequently observed with nuclear transformation and no …

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Limnological And Landscape Factors Affecting Use Of Manufactured Ponds By The Invasive Cuban Treefrog (Osteopilus Septentrionalis), Terina Nusinov Jan 2006

Limnological And Landscape Factors Affecting Use Of Manufactured Ponds By The Invasive Cuban Treefrog (Osteopilus Septentrionalis), Terina Nusinov

Electronic Theses and Dissertations

Exotic amphibians are often detrimental to native biotas. In Florida, the exotic Cuban Treefrog (Osteopilus septentrionalis) eats native frogs and may outcompete them for resources. Cuban Treefrogs thrive in disturbed areas and around buildings, and often breed in manufactured wetlands such as retention ponds and borrow pits. This study identified limnological, landscape, and biotic characteristics that discouraged pond use by Cuban Treefrogs and promoted use by native amphibian species. I sampled natural and manufactured ponds in Orange County, Florida, for one year, using standard methods to estimate the species richness and relative abundance of amphibians and their potential fish and …

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Is The Exotic Brazilian Pepper, Schinus Terebinthifolius, A Threat To Mangrove Ecosystems In Florida?, Melinda Donnelly Jan 2006

Is The Exotic Brazilian Pepper, Schinus Terebinthifolius, A Threat To Mangrove Ecosystems In Florida?, Melinda Donnelly

Electronic Theses and Dissertations

Mangrove ecosystems are critical to Florida, providing economic resources to humans, and untold ecological resources to estuarine organisms. In Florida's estuaries, mangrove ecosystems have suffered significant losses due to natural and human disturbances; these disturbances potentially leave mangrove communities vulnerable to invasion by the opportunistic exotic, Schinus terebinthifolius (Brazilian pepper). Prior experiments have suggested that Schinus terebinthifolius is unable to survive under marine conditions and poses no long term threat to mangrove systems. However, this contradicts field observations where Schinus terebinthifolius was found growing in the intertidal zone of Mosquito Lagoon alongside three native species of mangroves, Rhizophora mangle, Avicennia …

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Predicting Risks Of Invasion Of Caulerpa Species In Florida, Christian Glardon Jan 2006

Predicting Risks Of Invasion Of Caulerpa Species In Florida, Christian Glardon

Electronic Theses and Dissertations

Invasions of exotic species are one of the primary causes of biodiversity loss on our planet (National Research Council 1995). In the marine environment, all habitat types including estuaries, coral reefs, mud flats, and rocky intertidal shorelines have been impacted (e.g. Bertness et al. 2001). Recently, the topic of invasive species has caught the public's attention. In particular, there is worldwide concern about the aquarium strain of the green alga Caulerpa taxifolia (Vahl) C. Agardh that was introduced to the Mediterranean Sea in 1984 from the Monaco Oceanographic Museum. Since that time, it has flourished in thousands of hectares of …

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Ancient Maya Afterlife Iconography: Traveling Between Worlds, Mosley Dianna Wilson Jan 2006

Ancient Maya Afterlife Iconography: Traveling Between Worlds, Mosley Dianna Wilson

Electronic Theses and Dissertations

The ancient Maya afterlife is a rich and voluminous topic. Unfortunately, much of the material currently utilized for interpretations about the ancient Maya comes from publications written after contact by the Spanish or from artifacts with no context, likely looted items. Both sources of information can be problematic and can skew interpretations. Cosmological tales documented after the Spanish invasion show evidence of the religious conversion that was underway. Noncontextual artifacts are often altered in order to make them more marketable. An example of an iconographic theme that is incorporated into the surviving media of the ancient Maya, but that is …

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Predation On The Eastern Oyster Crassostrea Virginica On Intertidal Reefs Affected By Recreational Boating, Jennifer Stiner Jan 2006

Predation On The Eastern Oyster Crassostrea Virginica On Intertidal Reefs Affected By Recreational Boating, Jennifer Stiner

Electronic Theses and Dissertations

Widely regarded as a keystone species and ecosystem engineer, the eastern oyster Crassostrea virginica plays a vital role in estuarine environments. Complex, three-dimensional oyster reefs act as havens for biodiversity and contribute to ecological processes. Recently, concern for this resource has arisen in Mosquito Lagoon, Florida, the southernmost limit along the Atlantic coast for undisturbed, intertidal reefs of C. virginica. Since the 1990s, intense recreational boating activity has caused atypical dead margins (mounds of disarticulated shells) to emerge on the seaward edges of oyster reefs located along major navigational channels. Once dead margins are formed, little is known about their …

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Reproductive Delay In The Female Cape Ground Squirrel (Xerus Inauris), Beth Pettitt Jan 2006

Reproductive Delay In The Female Cape Ground Squirrel (Xerus Inauris), Beth Pettitt

Electronic Theses and Dissertations

The Cape ground squirrel, Xerus inauris, is a highly social cooperative breeder that forms groups containing multiple breeding females. While the distribution of reproduction among group members is fairly even (i.e. exhibits low reproductive skew), previous studies of Cape ground squirrels suggest the reproductive development of sub-adult females is inhibited by the presence of adult breeding female group mates. As reproductive delay is known to be influenced by a number of different parameters, my goal was to determine if other factors affected the timing of sexual maturity, and if so, which factors are the most influential. In this study, I …

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Expression And Characterization Of Mycobacterium Paratuberculosis 19kda With Posttranslational Modification, Mitra Safavi-Khasraghi Jan 2006

Expression And Characterization Of Mycobacterium Paratuberculosis 19kda With Posttranslational Modification, Mitra Safavi-Khasraghi

Electronic Theses and Dissertations

Despite the fact that E. coli supports limited posttranslational modification, this bacterium has been universally used as the expression system of choice. Expression of modified proteins in E. coli may lead to expression of recombinant proteins that lack essential immunomodulatory or catalytic components essentials for infectious processes. Previously in our laboratory, pMptb#28 plasmid containing a 4.8 kb insert from M. paratuberculosis has been identified which expressed 16 kDa recombinant protein in E. coli and 19 kDa recombinant protein in Mycobacterium smegmatis. The objective of this study is to identify the ORF sequence, investigate possible posttranslational modification and characterize the protein …

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Monitoring A Potentially Stressful Situation In Captive Western Lowland Gorillas (Gorilla Gorilla Gorilla) Through Analysis Of Behavior And Urinary Cortisol, Douglas Skurski Jan 2006

Monitoring A Potentially Stressful Situation In Captive Western Lowland Gorillas (Gorilla Gorilla Gorilla) Through Analysis Of Behavior And Urinary Cortisol, Douglas Skurski

Electronic Theses and Dissertations

The concept of quantifying animal welfare has received much discussion, in various industries such as agriculture, laboratory, and zoological facilities. Behavioral, physical, and physiological indicators of welfare have previously been used to assess animal welfare; each having advantages and disadvantages, ranging from the practicality of data collection, to the validity of the data and how it is interpreted. Concurrent assessment of multiple measures is a more robust way to examine animal welfare, which utilizes the advantages of each measure, and provides additional information on which to base conclusions and animal care management decisions. This study used measures of behavior and …

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University Of Central Florida Ir 2005-2006 Fact Book, University Of Central Florida Jan 2006

University Of Central Florida Ir 2005-2006 Fact Book, University Of Central Florida

Historical Fact Book and Enrollment

Historical fact book and enrollment data for the University of Central Florida.

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Bioinformatic Analysis Of Solanaceae Chloroplast Genomes And Characterization Of An Arabidopsis Protein Disulfide Isomerase In Transgenic Tobacco Chloroplasts, Justin James Grevich Jan 2006

Bioinformatic Analysis Of Solanaceae Chloroplast Genomes And Characterization Of An Arabidopsis Protein Disulfide Isomerase In Transgenic Tobacco Chloroplasts, Justin James Grevich

Electronic Theses and Dissertations

Throughout history, traditional plant breeding has been used to provide resistance to pests, disease and other forms of environmental stress, as well as to increase yield and improve upon quality and processing attributes. Over the last decade, the advancement in sequencing technology and bioinformatic analysis has unleashed a wealth of knowledge about chloroplast genetic organization and evolution. The lack of complete plastid genome sequences is one of the major limitations in advancing plastid genetic engineering to other useful crops. This is due to the fact that plastid genome sequences are essential for the identification of endogenous regulatory sequences and optimal …

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Studies On The Novel Function Of Amyloid Precursor Protein In Glial Differentiation Of Neural Stem Cells, Young-Don Kwak Jan 2006

Studies On The Novel Function Of Amyloid Precursor Protein In Glial Differentiation Of Neural Stem Cells, Young-Don Kwak

Electronic Theses and Dissertations

Although amyloid β (Aβ) deposition has been a hallmark of Alzheimer’s disease (AD), the physiological function of amyloid precursor protein (APP) is not clear. Our results suggested that high concentration of APP induces glial differentiation while physiological level of APP promotes migration and differentiation of neural stem cell (HNSC). HNSCs were mainly differentiated into astrocytes when they are transplanted into APP transgenic mouse brain or treated with a high concentration of secreted-type APP (sAPP) in culture. Staurosporine (STS) induced a distinctive astrocytic morphology in NT-2/D1 neural progenitor cells with expressions of APP and astrocyte-specific markers, glial fibrillary acidic protein (GFAP), …

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Surviving Reality: Survivor & Parasocial Interaction, Pedro Davila-Rosado Jan 2006

Surviving Reality: Survivor & Parasocial Interaction, Pedro Davila-Rosado

Electronic Theses and Dissertations

Parasocial interaction is the name that Horton & Wohl coined to describe a viewer's attachmentent toward onscreen persona that they had never physically interacted with (1956). A. Rubin, Perse, & Powell (1985) continued the research and created the Parasocial Interaction Scale. The scale has become the standard in gauging parasocial interaction in various forms of media from soap operas to newscasts. The purpose of this study was top examine parasocial interaction and see if the concept could be applied to the current television trend of reality television. Simultaneously, the study also examined parasocial interaction and its possible connections to loneliness, …

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A Social Cognitive Approach Towards Understanding The Effects Of Popular Poker Television Shows On College Students, Marc Londo Jan 2006

A Social Cognitive Approach Towards Understanding The Effects Of Popular Poker Television Shows On College Students, Marc Londo

Electronic Theses and Dissertations

Tournament poker shows have become a leading ratings draw on American television. Since ESPN and the Travel Channel began airing their innovative poker shows in 2003, the game has reached a new following, particularly among college students. There are unique and psychologically significant factors that characterize the college population that make students particularly receptive to popular characterizations in media. This study investigates the potential exacerbating effect that these widely popular poker television shows have on the gambling behavior of college students. 444 college students completed a survey designed to assess gratifications sought through media along with measures of attitudes, gambling …

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Notopleural Mutations Enhance Defects In Imaginal Disc Epithelial Morphogenesis And Macrochete Elongation Associated With Mutations In The Stubble-Stubbloid Locus, Robert Ruggiero Jan 2006

Notopleural Mutations Enhance Defects In Imaginal Disc Epithelial Morphogenesis And Macrochete Elongation Associated With Mutations In The Stubble-Stubbloid Locus, Robert Ruggiero

Electronic Theses and Dissertations

The Stubble-stubbloid locus encodes a transmembrane serine protease (Stubble) necessary for the proper formation of sensory bristles, and the morphogenesis of leg and wing epithelia. Genetic and cell biological analysis indicate a role for Stubble in actin cytoskeletal dynamics and cell shape changes in developing epithelia and bristles. Previously reported genetic interactions between Stubble and the Rho1 signaling pathway suggest Stubble influences actin cytoskeleton dynamics in developing imaginal discs through interactions with the Rho1 pathway. This work will discuss a genetic screen conducted to further investigate the role of Stubble in bristle and imaginal disc morphogenesis. From 50,000 EMS-mutagenized chromosomes …

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A Compartive Analysis Of The Evolution Of Forest Management In The United States In General, With A Focus On Oregon, North Carolina, Patrick Kelly Jan 2006

A Compartive Analysis Of The Evolution Of Forest Management In The United States In General, With A Focus On Oregon, North Carolina, Patrick Kelly

Electronic Theses and Dissertations

The purpose of this paper is to evaluate and discuss the evolution of forest management practices in the United States. The paper discusses the trends in forest management that have occurred within the United States in general, and specifically within the western (Oregon) and southern (North Carolina and Florida) United States. The trends discussed include the three (3) to four (4) epochs of management and use that are generally accepted within the forest management literature, with the exception of North Carolina that is in the process of a fifth (5). The comparative analysis within the paper discusses the western model …

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Mapping And Characterization Of 18-5 And 12-5, Genes Which Potentially Link The Rhoa Signaling Pathway To The Ecdysone Response, Samuel Fox Jan 2006

Mapping And Characterization Of 18-5 And 12-5, Genes Which Potentially Link The Rhoa Signaling Pathway To The Ecdysone Response, Samuel Fox

Electronic Theses and Dissertations

Systemic steroid hormone and intracellular signaling pathways are known to act cooperatively during the development of vertebrate and invertebrate epithelia. However, the mechanism of this interaction is poorly understood. Morphogenesis of Drosophila leg imaginal disc epithelia is regulated both by the steroid hormone 20-hydroxyecdysone (ecdysone) and the RhoA GTPase signaling pathway. Recent evidence suggests that these pathways act cooperatively to control imaginal disc morphogenesis. Thus, leg imaginal disc morphogenesis is an excellent system in which to study the interaction of steroid hormone and intracellular signaling pathways. We have identified mutations in three genes, 12-5, 18-5, and 31-6, with roles in …

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Dispersal Behavior Of Mosquitofish (Gambusia Holbrooki), Shireen Alemadi Jan 2006

Dispersal Behavior Of Mosquitofish (Gambusia Holbrooki), Shireen Alemadi

Electronic Theses and Dissertations

Mosquitofish (Gambusia holbrooki) are native to the southeastern United States but invasive elsewhere, and are dominant predators in many ecosystems that they inhabit. Information on dispersal behavior will help better understand and predict mosquitofish metapopulation dynamics and invasions. I experimentally tested dispersal behavior of individual mosquitofish under a range of laboratory conditions relevant to field situations. Preliminary experiments showed that gender, lighting conditions, hunger and acclimation time did not significantly affect net dispersal rate. Power analysis based on this preliminary experiment determined that 6 replicate fish were sufficient for each subsequent experiment; I used 24 fish, and each fish was …

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A Common Component-Based Software Architecture For Military And Commercial Pc-Based Virtual Simulation, Joshua Lewis Jan 2006

A Common Component-Based Software Architecture For Military And Commercial Pc-Based Virtual Simulation, Joshua Lewis

Electronic Theses and Dissertations

Commercially available military-themed virtual simulations have been developed and sold for entertainment since the beginning of the personal computing era. There exists an intense interest by various branches of the military to leverage the technological advances of the personal computing and video game industries to provide low cost military training. By nature of the content of the commercial military-themed virtual simulations, a large overlap has grown between the interests, resources, standards, and technology of the computer entertainment industry and military training branches. This research attempts to identify these commonalities with the purpose of systematically designing and evaluating a common component-based …

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"A Superabundance Of Life": The Letters Of Marjorie K. Rawlings To Cliff And Gladys Lyons, Brent Kinser Jan 2006

"A Superabundance Of Life": The Letters Of Marjorie K. Rawlings To Cliff And Gladys Lyons, Brent Kinser

The Marjorie Kinnan Rawlings Journal of Florida Literature

No abstract provided.

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User-Centered Virtual Environment Assessment And Design For Cognitive Rehabilitation Applications, Cali Fidopiastis Jan 2006

User-Centered Virtual Environment Assessment And Design For Cognitive Rehabilitation Applications, Cali Fidopiastis

Electronic Theses and Dissertations

Virtual environment (VE) design for cognitive rehabilitation necessitates a new methodology to ensure the validity of the resulting rehabilitation assessment. We propose that benchmarking the VE system technology utilizing a user-centered approach should precede the VE construction. Further, user performance baselines should be measured throughout testing as a control for adaptive effects that may confound the metrics chosen to evaluate the rehabilitation treatment. To support these claims we present data obtained from two modules of a user-centered head-mounted display (HMD) assessment battery, specifically resolution visual acuity and stereoacuity. Resolution visual acuity and stereoacuity assessments provide information about the image quality …

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A Holistic Usability Framework For Distributed Simulation Systems, Jeffrey Dawson Jan 2006

A Holistic Usability Framework For Distributed Simulation Systems, Jeffrey Dawson

Electronic Theses and Dissertations

This dissertation develops a holistic usability framework for distributed simulation systems (DSSs). The framework is developed considering relevant research in human-computer interaction, computer science, technical writing, engineering, management, and psychology. The methodology used consists of three steps: (1) framework development, (2) surveys of users to validate and refine the framework, and to determine attribute weights, and (3) application of the framework to two real-world systems. The concept of a holistic usability framework for DSSs arose during a project to improve the usability of the Virtual Test Bed, a prototypical DSS, and the framework is partly a result of that project. …

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Automated Scenario Generation System In A Simulation, Hajime Tomizawa Jan 2006

Automated Scenario Generation System In A Simulation, Hajime Tomizawa

Electronic Theses and Dissertations

Developing training scenarios that induce a trainee to utilize specific skills is one of the facets of simulation-based training that requires significant effort. Simulation-based training systems have become more complex in recent years. Because of this added complexity, the amount of effort required to generate and maintain training scenarios has increased. This thesis describes an investigation into automating the scenario generation process. The Automated Scenario Generation System (ASGS) generates expected action flow as contexts in chronological order from several events and tasks with estimated time for the entire training mission. When the training objectives and conditions are defined, the ASGS …

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Performance Efficacy Using A Comparison Of Commerical And In-House Y-Str Multiplex Systems For Operational Use, Kathleen Mayntz-Press Jan 2006

Performance Efficacy Using A Comparison Of Commerical And In-House Y-Str Multiplex Systems For Operational Use, Kathleen Mayntz-Press

Electronic Theses and Dissertations

It is routine for the forensic scientist to obtain a genetic profile of an individual from DNA recovered from a biological stain deposited at a crime scene. In contrast, only a limited number of laboratories in the United States have the capability of performing Y-STR analysis in casework. In order to aid in facilitating the transfer of Y-STR technology to the crime laboratory community for operational use, a comparison between commercial products from three main vendors (Applied Biosystems AmpFLSTR Yfiler PCR Amplification Kit, Promega PowerPlex - Y System, Reliagene Y-PLEX 12) and two in-house Y-STR multiplexes (MPI and MPB) commenced. …

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Examining Route Diversion And Multiple Ramp Metering Strategies For Reducing Real-Time Crash Risk On Urban Freeways, Vikash Gayah Jan 2006

Examining Route Diversion And Multiple Ramp Metering Strategies For Reducing Real-Time Crash Risk On Urban Freeways, Vikash Gayah

Electronic Theses and Dissertations

Recent research at the University of Central Florida addressing crashes on Interstate-4 in Orlando, Florida has led to the creation of new statistical models capable of calculating the crash risk on the freeway (Abdel-Aty et al., 2004; 2005, Pande and Abdel-Aty, 2006). These models yield the rear-end and lane-change crash risk along the freeway in real-time by using static information at various locations along the freeway as well as real-time traffic data that is obtained from the roadway. Because these models use the real-time traffic data, they are capable of calculating the respective crash risk values as the traffic flow …

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Cognitive Training Transfer Using A Personal Computer-Based Game:A Close Quarters Battle Case Study, Michael Woodman Jan 2006

Cognitive Training Transfer Using A Personal Computer-Based Game:A Close Quarters Battle Case Study, Michael Woodman

Electronic Theses and Dissertations

Developers of Commercial off the Shelf (COTS) gaming software are making remarkable strides in increasing the realism of their software. This realism has caught the attention of the training community, which has traditionally sought system and operational replication in training systems such as flight simulators. Since games are designed and marketed for entertainment and not designed for training of tasks, questions exist about the effectiveness of games as a training system in achieving the desired transfer of skills to the actual environment. Numerous studies over the past ten years have documented that PC-based simulation training environments can offer effective training …

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Fundamental Aspects Of Regenerative Cerium Oxide Nanoparticles And Their Applications In Nanobiotechnology, Swanand Patil Jan 2006

Fundamental Aspects Of Regenerative Cerium Oxide Nanoparticles And Their Applications In Nanobiotechnology, Swanand Patil

Electronic Theses and Dissertations

Cerium oxide has been used extensively for various applications over the past two decades. The use of cerium oxide nanoparticles is beneficial in present applications and can open avenues for future applications. The present study utilizes the microemulsion technique to synthesize uniformly distributed cerium oxide nanoparticles. The same technique was also used to synthesize cerium oxide nanoparticles doped with trivalent elements (La and Nd). The fundamental study of cerium oxide nanoparticles identified variations in properties as a function of particle size and also due to doping with trivalent elements (La and Nd). It was found that the lattice parameter of …

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Modeling Autonomous Agents In Military Simulations, Varol Kaptan Jan 2006

Modeling Autonomous Agents In Military Simulations, Varol Kaptan

Electronic Theses and Dissertations

Simulation is an important tool for prediction and assessment of the behavior of complex systems and situations. The importance of simulation has increased tremendously during the last few decades, mainly because the rapid pace of development in the field of electronics has turned the computer from a costly and obscure piece of equipment to a cheap ubiquitous tool which is now an integral part of our daily lives. While such technological improvements make it easier to analyze well-understood deterministic systems, increase in speed and storage capacity alone are not enough when simulating situations where human beings and their behavior are …

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Characterization Of Critical Network Components Of Coupled Oscillators, Gregory Holifield Jan 2006

Characterization Of Critical Network Components Of Coupled Oscillators, Gregory Holifield

Electronic Theses and Dissertations

This dissertation analyzes the fundamental limits for the determination of the network structure of loosely coupled oscillators based on observing the behavior of the network, specifically, node synchronization. The determination of the requisite characteristics and underlying behaviors necessary for the application of a theoretical mechanism for determining the underlying network topology in a network of loosely coupled natural oscillators are the desired outcome. To that end, this effort defines an analytical framework where key components of networks of coupled oscillators are isolated in order to determine the relationships between the various components. The relationship between the number of nodes in …

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Parameter Estimation In Linear Regression, Kati Ollikainen Jan 2006

Parameter Estimation In Linear Regression, Kati Ollikainen

Electronic Theses and Dissertations

Today increasing amounts of data are available for analysis purposes and often times for resource allocation. One method for analysis is linear regression which utilizes the least squares estimation technique to estimate a model's parameters. This research investigated, from a user's perspective, the ability of linear regression to estimate the parameters' confidence intervals at the usual 95% level for medium sized data sets. A controlled environment using simulation with known data characteristics (clean data, bias and or multicollinearity present) was used to show underlying problems exist with confidence intervals not including the true parameter (even though the variable was selected). …

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