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Waiving Miranda, Vance E. Voyles Jan 2011

Waiving Miranda, Vance E. Voyles

Electronic Theses and Dissertations

Waiving Miranda is a nonfiction collection that explores my career in law enforcement with a special emphasis on how the day-to-day association with others can lure a person into self-observation. The essays include my experiences as a road-patrol deputy, sex-crimes detective, and homicide detective in one of the largest county law enforcement agencies in the nation. Instead of the TV version of law enforcement— anecdotes of police chases and shoot outs—this thesis examines people on both sides of the yellow crimes scene tape as they face their own mortality and the gruesome truth of people’s unabashed cruelty towards one another. …

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A Laudable Ambition Fired Her Soul Conduct Fiction Helps Define Republican Womanhood, Female Communities, And Women's Education In The Works Of Judith Sargent Murray, Hannah Webster Foster, And Susanna Haswell Rowson, Jessica Crystal Workman Jan 2011

A Laudable Ambition Fired Her Soul Conduct Fiction Helps Define Republican Womanhood, Female Communities, And Women's Education In The Works Of Judith Sargent Murray, Hannah Webster Foster, And Susanna Haswell Rowson, Jessica Crystal Workman

Electronic Theses and Dissertations

This study examines the major works of Judith Sargent Murray, Hannah Webster Foster, and Susanna Haswell Rowson, three major writers of the 1790s whose writing responds to the ideologies of the early American Republic. I suggest that Murray, Foster, and Rowson write conduct fiction which responds to the changing attitudes toward women and education after the American Revolution. Using fiction, these authors comment on the republican woman, the need for women’s education, and the necessity for women to gather in communities for support. Despite the prevailing notion that reading too many novels would corrupt young women, Judith Sargent Murray’s novella, …

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Stable Expression Of Tuberculosis Vaccine Antigen In Lettuce Chloroplasts, Priya Saikumar Lakshmi Jan 2011

Stable Expression Of Tuberculosis Vaccine Antigen In Lettuce Chloroplasts, Priya Saikumar Lakshmi

Electronic Theses and Dissertations

Tuberculosis (TB) is caused by Mycobacterium tuberculosis and is one of the leading reasons of death by an infectious bacterial pathogen. The development of TB vaccines has been recognized as a major public health priority by the World Health Organization. In this study, a potential candidate antigen, ESAT-6 (6 kDa early secretory antigenic target) was fused with cholera toxin B subunit (CTB). Transplastomic lettuce plants were generated expressing these fusion proteins. Site-specific transgene integration into the chloroplast genome was confirmed by polymerase chain reaction and Southern blot analysis. In transplastomic leaves, expression levels of fusion protein (CTB-ESAT6) varied depending upon …

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An Uncurling Hand Isolation In Public Places, Kimberly Kelley Lundblom Jan 2011

An Uncurling Hand Isolation In Public Places, Kimberly Kelley Lundblom

Electronic Theses and Dissertations

The creative thesis ―An Uncurling Hand: Isolation in Public Places‖ is a collection of poetry concerned with ideological dichotomies: conventional domestication against the exotic, class divides and its implications for identity, and most importantly the feeling of isolation even when surrounded by others.

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Indirect Estimates Of Gene Flow And Its Conservation Implications In The Striped Newt (Notophthalmus Perstriatus), Sarah Elizabeth May Jan 2011

Indirect Estimates Of Gene Flow And Its Conservation Implications In The Striped Newt (Notophthalmus Perstriatus), Sarah Elizabeth May

Electronic Theses and Dissertations

This study used indirect methods to estimate patterns of gene flow in a rare salamander species, the striped newt (Notophthalmus perstriatus). First, we used combined genetic and ecological methods to determine whether populations that appear to exist in two regions separated by 125 km, exhibited genetic and ecological distinctness such that the regions demarcate separate conservation units. Using mtDNA (cyt-b), we found that haplotypes were shared between localities within each region but none were shared between regions. Niche-based distribution modeling revealed significant differences in the ecological setting between the two regions. In combination, the absence of evidence for recent genetic …

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Spiritual Redemption In A World Of Crime: Carolina Garcia-Aguilera And Barbara Parker, M B. Mclatchey Jan 2011

Spiritual Redemption In A World Of Crime: Carolina Garcia-Aguilera And Barbara Parker, M B. Mclatchey

The Marjorie Kinnan Rawlings Journal of Florida Literature

No abstract provided.

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Florida Stories, Mary W. Bridgman Jan 2011

Florida Stories, Mary W. Bridgman

The Marjorie Kinnan Rawlings Journal of Florida Literature

No abstract provided.

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Comparing Media Coverage Of The Gulf Oil Spill In The Us And Uk Implications For Global Crisis Communication, Sarah Crytzer Jan 2011

Comparing Media Coverage Of The Gulf Oil Spill In The Us And Uk Implications For Global Crisis Communication, Sarah Crytzer

Electronic Theses and Dissertations

The following research is a content analysis of 114 articles written by the American and British news media outlets in the first month following the BP Gulf oil spill in April 2010. The goal of the research was to identify any dominant frames evident in the reports and to compare the two countries to see if there was a difference in the dominant frames used. Positive, negative, and neutral tones were also evaluated to determine if there was a difference between the countries. The results show that both countries reports predominantly used an ecology and action frame, while British media …

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Hybrid Adult Neuron Culture Systems For Use In Pharmacological Testing, Darin Keay Edwards Jan 2011

Hybrid Adult Neuron Culture Systems For Use In Pharmacological Testing, Darin Keay Edwards

Electronic Theses and Dissertations

Neuronal culture systems have many applications, such as basic research into neuronal structure, function, and connectivity as well as research into diseases, conditions, and injuries affecting the brain and its components. In vitro dissociated neuronal systems have typically been derived from embryonic brain tissue, most commonly from the hippocampus of E18 rats. This practice has been motivated by difficulties in supporting regeneration, functional recovery and long-term survival of adult neurons in vitro. The overall focus of this dissertation research was to develop a dissociated neuronal culture system from human and animal adult brain tissue, one more functionally and developmentally correlative …

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Characterization Of The Nonlinear Refractive Index Of Carbon Disulfide Over An Extended Spectral And Temporal Range, Marcus Seidel Jan 2011

Characterization Of The Nonlinear Refractive Index Of Carbon Disulfide Over An Extended Spectral And Temporal Range, Marcus Seidel

Electronic Theses and Dissertations

The intensity dependent refractive index change of a medium is frequently described in terms of the product n₂ · I where n₂ is the nonlinear refractive index and I the light intensity. The nonlinear refractive index is often treated as constant which is a reasonable assumption if the light interacts only with bound electrons. In the case of carbon disulfide (CS₂) however, nuclear motions contribute to n₂. These motions occur on the sub picosecond time scale and thus become especially relevant for ultrashort laser pulses. The neat liquid CS₂ is studied because it exhibits a large nonlinear refractive index in …

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Discovery And Optimization Of Novel Small-Molecular Inhibitors Suppressing Stat3-Dependent Tumor Process, Xiaolei Zhang Jan 2011

Discovery And Optimization Of Novel Small-Molecular Inhibitors Suppressing Stat3-Dependent Tumor Process, Xiaolei Zhang

Electronic Theses and Dissertations

With the critical role of aberrantly active Signal Transducer and Activator of Transcription (Stat) 3 protein in many human cancers, selective small-molecule inhibitors targeting the dimerization event which is required for stat3 activation, would be valuable as therapeutic agents. And the inhibitors will be useful chemical probes to clarify the complex biological functions of Stat3. By computational and structural analyses of the interaction between Stat3 and the lead dimerization disruptor, S3I-201, we have designed a diverse set of analogs. One of the most active analogs, S3I-201.1066 is derived to contain a cyclo-hexyl benzyl moiety on the amide nitrogen, which increases …

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Impact Of Land Management On House Mice And Red Imported Fire Ants, Jesse R. Abelson Jan 2011

Impact Of Land Management On House Mice And Red Imported Fire Ants, Jesse R. Abelson

Electronic Theses and Dissertations

Understanding of mechanisms that limit the abundance and distribution of species is central to ecology. The failure of mechanisms to regulate populations can result in population outbreaks. There have been two outbreaks of house mice in the past decade in central Florida. In my study, I examine the efficacy of landscape management in the form of mowing and plowed soil barriers to limit or prevent outbreaks of house mice in a former agricultural area. House mouse populations were highly variable, but were unaffected by mowing or plowed soil barriers. Red imported fire ants were ubiquitous in the study area regardless …

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The Application Of Two-Photon Absorbing Probes In Lysosomal, Zinc Ion Sensing And Folate Receptor-Targeted Bioimaging, Xuhua Wang Jan 2011

The Application Of Two-Photon Absorbing Probes In Lysosomal, Zinc Ion Sensing And Folate Receptor-Targeted Bioimaging, Xuhua Wang

Electronic Theses and Dissertations

Two-photon fluorescence microscopy (2PFM) has become a powerful technique for bioimaging in non-invasive cancer diagnosis and also investigating the mechanization and original of a variety of diseases by tracking various biological processes. Because the fluorescence emission by two photon absorbing (2PA) is directly proportional to the square of the intensity of excitation light, this intrinsic property of 2PA provides 2PFM great advantages over traditional one-photon fluorescence microscopy (1PFM), including high 3D spatial localization, less photodamage and interference from biological tissue because of using longer wavelength excitation (700-1300 nm). However, most 2PA probes are hydrophobic and their photostabilities are questionable, severely …

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Genetic And Physiological Contribution Of Adrenergic Cells In Heart Development, Kingsley Osuala Jan 2011

Genetic And Physiological Contribution Of Adrenergic Cells In Heart Development, Kingsley Osuala

Electronic Theses and Dissertations

The adrenergic hormones norepinephrine (NE) and epinephrine (EPI) are essential for cardiovascular development as embryos lacking NE/EPI begin to die abruptly between embryonic days 10.5 and 11.5 due to apparent cardiac failure. The objective of this research aims to elucidate the mechanism of the embryonic fatality observed in the NE/EPI deficient mouse model. Here we utilized the dopamine β- hydroxylase knockout (Dbh-/-) mouse model, which lacks the gene and subsequent enzyme necessary for the conversion of dopamine to norepinephrine. Embryonic mouse hearts were extracted from Dbh+/+ (control) and Dbh-/- (experimental model) mice for mRNA transcript expression profiling. These studies were …

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The Stigmatization Of Deathcare Workers, Shannon Rose Simone Jan 2011

The Stigmatization Of Deathcare Workers, Shannon Rose Simone

Electronic Theses and Dissertations

Research has indicated that Funeral Directors and Embalmers are stigmatized for their work. Studies have shown that, although the decay of dead bodies is a stigmatized process in American culture, these particular deathcare workers are able to shift the focus of their services from the dead to the living. However, there remains a lack of research regarding deathcare workers who are not employed as Funeral Directors or Embalmers— those whose positions are not as obvious to the general public. This research explores how, why, and to what degree stigma is placed on those individuals who spend the majority of their …

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Innovation On A Budget The Development Of Military Technology During The Interwar Period, 1919-1939, William Erik Deupree Jan 2011

Innovation On A Budget The Development Of Military Technology During The Interwar Period, 1919-1939, William Erik Deupree

Electronic Theses and Dissertations

This thesis investigates the progress of technological development during the interwar period of 1919 to 1939. The interwar period was a time of slashed military budgets and isolationist policies. However, despite political, financial, and organizational handicaps, each branch of the military made significant progress in the development of military technology, and the air corps and navy achieved significantly better results. The reason these two branches were able succeed was through a combination of organizational policy and the development of an overarching goal for their respective branch. Within this thesis, I investigated each of the major military branches during the interwar …

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Supporting Hispanic Mothers With Preschool Children With Speech And/ Or Language Delays Via Dialogic Reading And Coaching Within The Home, Natalie Elizabeth Dopson Jan 2011

Supporting Hispanic Mothers With Preschool Children With Speech And/ Or Language Delays Via Dialogic Reading And Coaching Within The Home, Natalie Elizabeth Dopson

Electronic Theses and Dissertations

Young children who are Hispanic, from low-income homes and have developmental delays are at a disadvantage for not having the basic early literacy foundation to become successful readers later in school (Ballantyne, Sanderman, D‘Emilio, & McLaughlin, 2008; Hammer, Farkas, & Maczuga, 2010; Ezell & Justice 2005; McCardle, Scarborough, & Catts, 2001). These challenges can be addressed in several ways. Early intervention including parent education and collaboration along with shared book reading are considered best practices and critical to improving child outcomes (NELP, 2008). In addition, children who have a solid foundation in early literacy skills including vocabulary development in their …

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Towards Calibration Of Optical Flow Of Crowd Videos Using Observed Trajectories, Iman K. Elbadramany Jan 2011

Towards Calibration Of Optical Flow Of Crowd Videos Using Observed Trajectories, Iman K. Elbadramany

Electronic Theses and Dissertations

The need exists for finding a quantitative method for validating crowd simulations. One approach is to use optical flow of videos of real crowds to obtain velocities that can be used for comparison to simulations. Optical flow, in turn, needs to be calibrated to be useful. It is essential to show that optical flow velocities obtained from crowd videos can be mapped into the spatially averaged velocities of the observed trajectories of crowd members, and to quantify the extent of the correlation of the results. This research investigates methods to uncover the best conditions for a good correlation between optical …

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Genetically Modified Es Cells Enhance Cardiac Repair And Regeneration In The Infarcted Heart, Carley E. Glass Jan 2011

Genetically Modified Es Cells Enhance Cardiac Repair And Regeneration In The Infarcted Heart, Carley E. Glass

Electronic Theses and Dissertations

Transplanted embryonic stem (ES) cells following myocardial infarction (MI) contribute to limited cardiac repair and regeneration with improved function. Therefore novel strategies are still needed to enhance the efficacy by which ES cells differentiate into cardiac cell types and inhibit adverse remodeling in the infarcted myocardium. Our studies evaluate whether genetic manipulation of transplanted ES cells employing miR- 1, a pro-cardiac microRNA, and TIMP-1, an anti-apoptotic and anti-fibrotic protein, will enhance cardiac myocyte differentiation, inhibit native cardiac apoptosis, and reduce fibrosis in the infarcted myocardium. Furthermore, we assess levels of associated pro- (caspase-3, PTEN) and anti-(Akt) apoptotic proteins as well …

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The Relative Recoverability Of Dna And Rna Profiles From Forensically Relevant Body Fluid Stains, Charly Parker Jan 2011

The Relative Recoverability Of Dna And Rna Profiles From Forensically Relevant Body Fluid Stains, Charly Parker

Electronic Theses and Dissertations

Biological material (fluids or tissues) whether from the victim or suspect is often collected as forensic evidence, and methods to obtain and analyze the DNA found in that material have been well established. The type of body fluid (i.e. blood, saliva, semen, vaginal secretions, and menstrual blood) from which the DNA originated is also of interest, and messenger RNA typing provides a specific and sensitive means of body fluid identification. In order for mRNA profiling to be utilized in routine forensic casework, RNA of sufficient quantity and quality must be obtained from biological fluid stains and the methods used for …

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Emotional Regulation At Walt Disney World Deep Acting Vs. Surface Acting, Anne Reyers Jan 2011

Emotional Regulation At Walt Disney World Deep Acting Vs. Surface Acting, Anne Reyers

Electronic Theses and Dissertations

The objective of this study is to examine the emotional regulation strategies used by Walt Disney World on-stage employees as a way to fulfill requirements set forth by the company. Ten Disney on-stage employees were interviewed off-property in Orlando. The emotional regulation framework was divided into several categories: (1) a distinction between deep acting and surface acting, (2) emotional deviance, and (3) emotional exhaustion. “Surface acting” is a strategy by which employees display company-imposed emotions not genuinely felt, whereas “deep acting” occurs when employees do feel the emotions that they are required to express (Hochschild, 1983). Throughout the data reduction …

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Investigating The Optimal Presentation Of Feedback In Simulation-Based Training An Application Of The Cognitive Theory Of Multimedia Learning, Wendi Van Buskirk Jan 2011

Investigating The Optimal Presentation Of Feedback In Simulation-Based Training An Application Of The Cognitive Theory Of Multimedia Learning, Wendi Van Buskirk

Electronic Theses and Dissertations

There are many different training interventions that can be used in simulation based training systems (e.g., cueing, hinting, highlighting, deliberate practice, etc.). However, the most widely used training intervention in the military is feedback, most often presented in the form of a debrief. With advances in technology, it is possible to measure and diagnose performance in real-time. Thus it is possible to provide immediate feedback during scenarios. However, training systems designers should not consider the timing of feedback in isolation. There are other parameters of feedback that must also be considered which may have an impact on performance. Specifically, feedback …

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Predicting Intentions To Donate To Human Service Nonprofits And Public Broadcasting Organizations Using A Revised Theory Of Planned Behavior, Bobbie Brinkerhoff Jan 2011

Predicting Intentions To Donate To Human Service Nonprofits And Public Broadcasting Organizations Using A Revised Theory Of Planned Behavior, Bobbie Brinkerhoff

Electronic Theses and Dissertations

Different types of nonprofit organizations including human service nonprofits like homeless shelters, public broadcasting organizations, and the like thrive on donations. Effective fundraising techniques are essential to a nonprofit’s existence. This research study explored a revised theory of planned behavior to include guilt and convenience in order to understand whether these factors are important in donors’ intentions to give. This study also examined the impact of two different kinds of guilt; anticipated guilt and existential guilt to determine if there was any difference between the types of guilt and the roles that they play as predicting factors in a revised …

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Worldview, Ideology, And Ceramic Iconography A Study Of Late Terminal Formative Graywares From The Lower Rio Verde Valley Of Oaxaca, Mexico, Jeffrey S. Brzezinski Jan 2011

Worldview, Ideology, And Ceramic Iconography A Study Of Late Terminal Formative Graywares From The Lower Rio Verde Valley Of Oaxaca, Mexico, Jeffrey S. Brzezinski

Electronic Theses and Dissertations

This study investigates worldview and ideology during the late Terminal Formative period (A.D. 100 – 250) in the lower Río Verde Valley of Oaxaca, Mexico, through an analysis of iconography found on grayware ceramic serving vessels. The sample includes 457 vessels and sherds from 17 lower Verde sites obtained through excavations and surface collections between 1988 and 2009. Drawing upon theories of semiotics and style, this thesis identifies a suite of icons suggesting that ceramics were a medium for expressing regionally shared beliefs. Chatino potters carved common Formative period Mesoamerican themes into the walls of graywares, such as depictions of …

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Laser Induced Breakdown Spectroscopy For Detection Of Organic Residues Impact Of Ambient Atmosphere And Laser Parameters, Christopher G. Brown Jan 2011

Laser Induced Breakdown Spectroscopy For Detection Of Organic Residues Impact Of Ambient Atmosphere And Laser Parameters, Christopher G. Brown

Electronic Theses and Dissertations

Laser Induced Breakdown Spectroscopy (LIBS) is showing great potential as an atomic analytical technique. With its ability to rapidly analyze all forms of matter, with little-to-no sample preparation, LIBS has many advantages over conventional atomic emission spectroscopy techniques. With the maturation of the technologies that make LIBS possible, there has been a growing movement to implement LIBS in portable analyzers for field applications. In particular, LIBS has long been considered the front-runner in the drive for stand-off detection of trace deposits of explosives. Thus there is a need for a better understanding of the relevant processes that are responsible for …

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The Effects Of Age On Reproduction In A Citrus Root Weevil Diaprepes Abbreviatus, Heather R. Chasez Jan 2011

The Effects Of Age On Reproduction In A Citrus Root Weevil Diaprepes Abbreviatus, Heather R. Chasez

Electronic Theses and Dissertations

Understanding the factors influencing mate choice is a major focus of sexual selection. Many factors are potentially involved, including age of the individual. The good genes model and the youth advantage model both make predictions about the effect of age on mate choice. Under the good genes model older mates would be the more preferable due to their proven high survivability. The “youth advantage” model, predicts that young to intermediate age males would be more advantageous as mates because of a decrease in sperm quality and the possibility of increased germ-line mutations in older animals. I examined the effects of …

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Glial Differentiation Of Human Umbilical Stem Cells In 2d And 3d Environments, Hedvika Davis Jan 2011

Glial Differentiation Of Human Umbilical Stem Cells In 2d And 3d Environments, Hedvika Davis

Electronic Theses and Dissertations

During differentiation stem cells are exposed to a range of microenvironmental chemical and physical cues. In this study, human multipotent progenitor cells (hMLPCs) were differentiated from umbilical cord into oligodendrocytes and astrocytes. Chemical cues were represented by a novel defined differentiation medium containing the neurotransmitter norepinephrine (NE). In traditional 2 dimensional (2D) conditions, the hMLPCs differentiated into oligodendrocyte precursors, but did not progress further. However, in a constructed 3 dimensional (3D) environment, the hMLPCs differentiated into committed oligodendrocytes that expressed MBP. When co-cultured with rat embryonic hippocampal neurons (EHNs), hMLPCs developed in astrocytes or oligodendrocytes, based on presence of growth …

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The Vegetarian Social Movement An Analysis Of Withdrawal And Backsliding, Jaime Deborah Hecht Jan 2011

The Vegetarian Social Movement An Analysis Of Withdrawal And Backsliding, Jaime Deborah Hecht

Electronic Theses and Dissertations

The vegetarian social movement is a “new” social movement based in lifestyle and cultural change. New social movements hold a strong emphasis on collective identity and social networks as a means to sustain participation. The majority of the social movement literature remains focused on movement engagement and mobilization while a large gap exists regarding disengagement. This project explores the barriers to vegetarian maintenance. The primary question answered is, why do some vegetarians and vegans backslide and withdraw from the practice? Fourteen individuals were interviewed to discover the social and cultural factors inherent in vegetarian instability. Over the course of the …

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The Lived Experience Of Young-Onset Dementia, Debra A. Hunt Jan 2011

The Lived Experience Of Young-Onset Dementia, Debra A. Hunt

Electronic Theses and Dissertations

The lived experiences of dementia in older persons have been well studied, but the unique experiences of persons between ages 35 and 65 years who are living with young-onset dementia have not been closely examined. The purpose of the research was to explore the experiences of middle-aged individuals living with young-onset dementia. Van Manen‘s (1990) approach to interpretive phenomenological inquiry was used to answer the research question. Purposive sampling was used to recruit 9 people between 42 to 61 years of age who had received a formal diagnosis of mild or early-stage dementia. Participants were prescreened for the ability to …

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The Origins Of Lactase Persistence And Ongoing Convergent Evolution, Beth A. Keller Jan 2011

The Origins Of Lactase Persistence And Ongoing Convergent Evolution, Beth A. Keller

Electronic Theses and Dissertations

As a primary factor in human evolution, natural selection is an important component of genetic research. Studies of lactase persistence suggest that positive selection has played a powerful role in the adaptation to a lifelong consumption of fresh milk. Using multiple research studies of lactase persistence and suspected corresponding single nucleotide genetic polymorphisms, this study combines data sources to determine whether evidence exists for natural selection of a specific cytosine-to-thymine genetic mutation located 13,910 base pairs (T-13910) upstream from the lactase gene. This polymorphism has potential to be a causal element for lactase persistence, and data suggest that natural selection …

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