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Low Cost Tuberculosis Vaccine Antigens In Capsules: Expression In Chloroplasts, Bio-Encapsulation, Stability And Functional Evaluation In Vitro, Priya Saikumar Lakshmi, Dheeraj Verma, Xiangdong Yang, Bethany Lloyd, Hnery Daniell Jan 2013

Low Cost Tuberculosis Vaccine Antigens In Capsules: Expression In Chloroplasts, Bio-Encapsulation, Stability And Functional Evaluation In Vitro, Priya Saikumar Lakshmi, Dheeraj Verma, Xiangdong Yang, Bethany Lloyd, Hnery Daniell

Faculty Bibliography 2010s

Tuberculosis (TB) caused by Mycobacterium tuberculosis is one of the leading fatal infectious diseases. The development of TB vaccines has been recognized as a major public health priority by the World Health Organization. In this study, three candidate antigens, ESAT-6 (6kDa early secretory antigenic target) and Mtb72F (a fusion polyprotein from two TB antigens, Mtb32 and Mtb39) fused with cholera toxin B-subunit (CTB) and LipY (a cell wall protein) were expressed in tobacco and/or lettuce chloroplasts to facilitate bioencapsulation/oral delivery. Site-specific transgene integration into the chloroplast genome was confirmed by Southern blot analysis. In transplastomic leaves, CTB fusion proteins existed …

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Erythropoietin Is A Jak2 And Erk1/2 Effector That Can Promote Renal Tumor Cell Proliferation Under Hypoxic Conditions, Makito Miyake, Steve Goodison, Adrienne Lawton, Ge Zhang, Evan Gomes-Giacoia, Charles J. Rosser Jan 2013

Erythropoietin Is A Jak2 And Erk1/2 Effector That Can Promote Renal Tumor Cell Proliferation Under Hypoxic Conditions, Makito Miyake, Steve Goodison, Adrienne Lawton, Ge Zhang, Evan Gomes-Giacoia, Charles J. Rosser

Faculty Bibliography 2010s

Background: Erythropoietin (EPO) provides an alternative to transfusion for increasing red blood cell mass and treating anemia in cancer patients. However, recent studies have reported increased adverse events and/or reduced survival in patients receiving both EPO and chemotherapy, potentially related to EPO-induced cancer progression. Additional preclinical studies that elucidate the possible mechanism underlying EPO cellular growth stimulation are needed. Methods: Using commercial tissue microarray (TMA) of a variety of cancers and benign tissues, EPO and EPO receptor immunohistochemical staining was performed. Furthermore using a panel of human renal cells (Caki-1, 786-O, 769-P, RPTEC), in vitro and in vivo experiments were …

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The Role Of Chemokine Receptor Cxcr4 In Breast Cancer Metastasis, Debarati Mukherjee, Jihe Zhao Jan 2013

The Role Of Chemokine Receptor Cxcr4 In Breast Cancer Metastasis, Debarati Mukherjee, Jihe Zhao

Faculty Bibliography 2010s

Breast cancer is one of the leading causes of cancer related deaths worldwide. Breast cancer-related mortality is associated with the development of metastatic potential of primary tumor lesions. The chemokine receptor CXCR4 has been found to be a prognostic marker in various types of cancer, including breast cancer. Recent advances in the field of cancer biology has pointed to the critical role that CXCR4 receptor and its ligand CXCL12 play in the metastasis of various types of cancer, including breast cancer. Breast tumors preferentially metastasize to the lung, bones and lymph nodes, all of which represent organs that secrete high …

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Training And Transfer Of Training In Rapid Visual Search For Camouflaged Targets, Mark B. Neider, Cher Wee Ang, Michelle W. Voss, Ronald Carbonari, Arthur F. Kramer Jan 2013

Training And Transfer Of Training In Rapid Visual Search For Camouflaged Targets, Mark B. Neider, Cher Wee Ang, Michelle W. Voss, Ronald Carbonari, Arthur F. Kramer

Faculty Bibliography 2010s

Previous examinations of search under camouflage conditions have reported that performance improves with training and that training can engender near perfect transfer to similar, but novel camouflage-type displays [1]. What remains unclear, however, are the cognitive mechanisms underlying these training improvements and transfer benefits. On the one hand, improvements and transfer benefits might be associated with higher-level overt strategy shifts, such as through the restriction of eye movements to target-likely (background) display regions. On the other hand, improvements and benefits might be related to the tuning of lower-level perceptual processes, such as figure-ground segregation. To decouple these competing possibilities we …

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Mapping Crmp3 Domains Involved In Dendrite Morphogenesis And Voltage-Gated Calcium Channel Regulation, Tam T. Quach, Sarah M. Wilson, Veronique Rogemond, Naura Chounlamountri, Pappachan E. Kolattukudy, Stephanie Martinez, May Khanna, Marie-Francoise Belin, Rajesh Khanna, Jerome Honnorat, Anne-Marie Duchemin Jan 2013

Mapping Crmp3 Domains Involved In Dendrite Morphogenesis And Voltage-Gated Calcium Channel Regulation, Tam T. Quach, Sarah M. Wilson, Veronique Rogemond, Naura Chounlamountri, Pappachan E. Kolattukudy, Stephanie Martinez, May Khanna, Marie-Francoise Belin, Rajesh Khanna, Jerome Honnorat, Anne-Marie Duchemin

Faculty Bibliography 2010s

Although hippocampal neurons are well-distinguished by the morphological characteristics of their dendrites and their structural plasticity, the mechanisms involved in regulating their neurite initiation, dendrite growth, network formation and remodeling are still largely unknown, in part because the key molecules involved remain elusive. Identifying new dendrite-active cues could uncover unknown molecular mechanisms that would add significant understanding to the field and possibly lead to the development of novel neuroprotective therapy because these neurons are impaired in many neuropsychiatric disorders. In our previous studies, we deleted the gene encoding CRMP3 in mice and identified the protein as a new endogenous signaling …

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Grape Extracts Inhibit Multiple Events In The Cell Biology Of Cholera Intoxication, Srikar Reddy, Michael Taylor, Mojun Zhao, Patrick Cherubin, Sandra Geden, Supriyo Ray, David Francis, Ken Teter Jan 2013

Grape Extracts Inhibit Multiple Events In The Cell Biology Of Cholera Intoxication, Srikar Reddy, Michael Taylor, Mojun Zhao, Patrick Cherubin, Sandra Geden, Supriyo Ray, David Francis, Ken Teter

Faculty Bibliography 2010s

Vibrio cholerae produces cholera toxin (CT), an AB(5) protein toxin that is primarily responsible for the profuse watery diarrhea of cholera. CT is secreted into the extracellular milieu, but the toxin attacks its Gs alpha target within the cytosol of a host cell. Thus, CT must cross a cellular membrane barrier in order to function. This event only occurs after the toxin travels by retrograde vesicular transport from the cell surface to the endoplasmic reticulum (ER). The catalytic A1 polypeptide then dissociates from the rest of the toxin and assumes an unfolded conformation that facilitates its transfer to the cytosol …

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A Vulnerability Assessment Of 300 Species In Florida: Threats From Sea Level Rise, Land Use, And Climate Change, Joshua Steven Reece, Reed F. Noss, Jon Oetting, Tom Hoctor, Michael Volk Jan 2013

A Vulnerability Assessment Of 300 Species In Florida: Threats From Sea Level Rise, Land Use, And Climate Change, Joshua Steven Reece, Reed F. Noss, Jon Oetting, Tom Hoctor, Michael Volk

Faculty Bibliography 2010s

Species face many threats, including accelerated climate change, sea level rise, and conversion and degradation of habitat from human land uses. Vulnerability assessments and prioritization protocols have been proposed to assess these threats, often in combination with information such as species rarity; ecological, evolutionary or economic value; and likelihood of success. Nevertheless, few vulnerability assessments or prioritization protocols simultaneously account for multiple threats or conservation values. We applied a novel vulnerability assessment tool, the Standardized Index of Vulnerability and Value, to assess the conservation priority of 300 species of plants and animals in Florida given projections of climate change, human …

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Electric Organ Discharge Diversity In The Genus Gymnotus: Anatomo-Functional Groups And Electrogenic Mechanisms, A. Rodríguez-Cattáneo, P. Aguilera, E. Cilleruelo, W. G. R. Crampton, A. A. Caputi Jan 2013

Electric Organ Discharge Diversity In The Genus Gymnotus: Anatomo-Functional Groups And Electrogenic Mechanisms, A. Rodríguez-Cattáneo, P. Aguilera, E. Cilleruelo, W. G. R. Crampton, A. A. Caputi

Faculty Bibliography 2010s

Previous studies describe six factors accounting for interspecific diversity of electric organ discharge (EOD) waveforms in Gymnotus. At the cellular level, three factors determine the locally generated waveforms: (1) electrocyte geometry and channel repertoire; (2) the localization of synaptic contacts on electrocyte surfaces; and (3) electric activity of electromotor axons preceding the discharge of electrocytes. At the organismic level, three factors determine the integration of the EOD as a behavioral unit: (4) the distribution of different types of electrocytes and specialized passive tissue forming the electric organ (EO); (5) the neural mechanisms of electrocyte discharge coordination; and (6) post-effector mechanisms. …

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Mycobacterial Trehalose Dimycolate Reprograms Macrophage Global Gene Expression And Activates Matrix Metalloproteinases, Kaori Sakamoto, Mi Jeong Kim, Elizabeth R. Rhoades, Rachel E. Allavena, Sabine Ehrt, Helen C. Wainwright, David G. Russell, Kyle H. Rohde Jan 2013

Mycobacterial Trehalose Dimycolate Reprograms Macrophage Global Gene Expression And Activates Matrix Metalloproteinases, Kaori Sakamoto, Mi Jeong Kim, Elizabeth R. Rhoades, Rachel E. Allavena, Sabine Ehrt, Helen C. Wainwright, David G. Russell, Kyle H. Rohde

Faculty Bibliography 2010s

Trehalose 6,6'-dimycolate (TDM) is a cell wall glycolipid and an important virulence factor of mycobacteria. In order to study the role of TDM in the innate immune response to Mycobacterium tuberculosis, microarray analysis was used to examine gene regulation in murine bone marrow-derived macrophages in response to 90-mu m-diameter polystyrene microspheres coated with TDM. A large number of genes, particularly those involved in the immune response and macrophage function, were up-or downregulated in response to these TDM-coated beads compared to control beads. Genes involved in the immune response were specifically upregulated in a myeloid differentiation primary response gene 88 (MyD88)-dependent …

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Immunomodulation And T Helper Th1/Th2 Response Polarization By Ceo2 And Tio2 Nanoparticles, Brian C. Schanen, Soumen Das, Christopher M. Reilly, William L. Warren, William T. Self, Sudipta Seal, Donald R. Drake Iii Jan 2013

Immunomodulation And T Helper Th1/Th2 Response Polarization By Ceo2 And Tio2 Nanoparticles, Brian C. Schanen, Soumen Das, Christopher M. Reilly, William L. Warren, William T. Self, Sudipta Seal, Donald R. Drake Iii

Faculty Bibliography 2010s

Immunomodulation by nanoparticles, especially as related to the biochemical properties of these unique materials, has scarcely been explored. In an in vitro model of human immunity, we demonstrate two catalytic nanoparticles, TiO2 (oxidant) and CeO2 (antioxidant), have nearly opposite effects on human dendritic cells and T helper (T-H) cells. For example, whereas TiO2 nanoparticles potentiated DC maturation that led towards T(H)1-biased responses, treatment with antioxidant CeO2 nanoparticles induced APCs to secrete the anti-inflammatory cytokine, IL-10, and induce a T(H)2-dominated T cell profile. In subsequent studies, we demonstrate these results are likely explained by the disparate capacities of the nanoparticles to …

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Novel Technique For Generating Macrophage Foam Cells For In Vitro Reverse Cholesterol Transport Studies, Bhaswati Sengupta, Chandrakala Aluganti Narasimhulu, Sampath Parthasarathy Jan 2013

Novel Technique For Generating Macrophage Foam Cells For In Vitro Reverse Cholesterol Transport Studies, Bhaswati Sengupta, Chandrakala Aluganti Narasimhulu, Sampath Parthasarathy

Faculty Bibliography 2010s

Generation of foam cells, an essential step for reverse cholesterol transport studies, uses the technique of receptor-dependent macrophage loading with radiolabeled acetylated LDL. In this study, we used the ability of a biologically relevant detergent molecule, lysophosphatidylcholine (lyso-PtdCho), to form mixed micelles with cholesterol or cholesteryl ester (CE) to generate macrophage foam cells. Fluorescent or radiolabeled cholesterol/lyso-PtdCho mixed micelles were prepared and incubated with RAW 264.7 or mouse peritoneal macrophages. Results showed that such micelles were quite stable at 4 degrees C and retained the solubilized cholesterol during one month of storage. Macrophages incubated with cholesterol or CE (unlabeled, fluorescently …

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Cideb Protein Is Required For The Biogenesis Of Very Low Density Lipoprotein (Vldl) Transport Vesicle, Samata Tiwari, Shaila Siddiqi, Shadab A. Siddiqi Jan 2013

Cideb Protein Is Required For The Biogenesis Of Very Low Density Lipoprotein (Vldl) Transport Vesicle, Samata Tiwari, Shaila Siddiqi, Shadab A. Siddiqi

Faculty Bibliography 2010s

Nascent very low density lipoprotein (VLDL) exits the endoplasmic reticulum (ER) in a specialized ER-derived vesicle, the VLDL transport vesicle (VTV). Similar to protein transport vesicles (PTVs), VTVs require coat complex II (COPII) proteins for their biogenesis from the ER membranes. Because the size of the VTV is large, we hypothesized that protein(s) in addition to COPII components might be required for VTV biogenesis. Our proteomic analysis, supported by Western blotting data, shows that a 26-kDa protein, CideB, is present in the VTV but not in other ER-derived vesicles such as PTV and pre-chylomicron transport vesicle. Western blotting and immunoelectron …

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The Role Of Habitat In The Persistence Of Fire Ant Populations, Walter R. Tschinkel, Joshua R. King Jan 2013

The Role Of Habitat In The Persistence Of Fire Ant Populations, Walter R. Tschinkel, Joshua R. King

Faculty Bibliography 2010s

The association of the exotic fire ant, Solenopsis invicta with man-modified habitats has been amply demonstrated, but the fate of such populations if ecological succession proceeds has rarely been investigated. Resurvey of a fire ant population in a longleaf pine plantation after 25 years showed that the recovery of the site from habitat disturbance was associated with a large fire ant population decline. Most of the persisting colonies were associated with the disturbance caused by vehicle tracks. In a second study, mature monogyne fire ant colonies that had been planted in experimental plots in native groundcover of the north Florida …

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Discovering Transcription And Splicing Networks In Myelodysplastic Syndromes, Hongyan Wang, Jianguo Wen, Chung-Che Chang, Xiaobo Zhou Jan 2013

Discovering Transcription And Splicing Networks In Myelodysplastic Syndromes, Hongyan Wang, Jianguo Wen, Chung-Che Chang, Xiaobo Zhou

Faculty Bibliography 2010s

More and more transcription factors and their motifs have been reported and linked to specific gene expression levels. However, focusing only on transcription is not sufficient for mechanism research. Most genes, especially in eukaryotes, are alternatively spliced to different isoforms. Some of these isoforms increase the biodiversity of proteins. From this viewpoint, transcription and splicing are two of important mechanisms to modulate expression levels of isoforms. To integrate these two kinds of regulation, we built a linear regression model to select a subset of transcription factors and splicing factors for each co-expressed isoforms using least-angle regression approach. Then, we applied …

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Kruppel-Like Factor 8 Promotes Tumorigenic Mammary Stem Cell Induction By Targeting Mir-146a, Xianhui Wang, Heng Lu, Tianshu Li, Lin Yu, Gang Liu, Xu Peng, Jihe Zhao Jan 2013

Kruppel-Like Factor 8 Promotes Tumorigenic Mammary Stem Cell Induction By Targeting Mir-146a, Xianhui Wang, Heng Lu, Tianshu Li, Lin Yu, Gang Liu, Xu Peng, Jihe Zhao

Faculty Bibliography 2010s

The properties of stem cells can be induced during the epithelial to mesenchymal transition (EMT). The responsible molecular mechanisms, however, remain largely undefined. Here we report the identification of the microRNA-146a (miR-146a) as a common target of Kruppel-like factor 8 (KLF8) and TGF-beta, both of which are known EMT-inducers. Upon KLF8 overexpression or TGF-beta treatment, a significant portion of the MCF-10A cells gained stem cell traits as demonstrated by an increased expression of CD44(high)/CD24(low), activity of aldehyde dehydrogenase (ALDH), mammosphere formation and chemoresistance. Along with this change, the expression of miR-146a was highly upregulated in the cells. Importantly, we found …

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Cerium Oxide Nanoparticles: Potential Applications For Cancer And Other Diseases, Melissa S. Wason, Jihe Zhao Jan 2013

Cerium Oxide Nanoparticles: Potential Applications For Cancer And Other Diseases, Melissa S. Wason, Jihe Zhao

Faculty Bibliography 2010s

The diverse abilities of cerium oxide nanoparticles (CONPs) have encouraged researchers to pursue CONPs as a therapeutic agent to treat a number of diseases, including cancer. In vitro and in vivo studies have shown CONPs to be toxic to cancer cells, inhibit invasion, and sensitize cancer cells to radiation therapy. However, CONPs display minimal toxicity to normal tissues and provide protection from various forms of reactive oxygen species (ROS) generation. The antioxidant capabilities of CONPs, which enable radiation protection, have also resulted in the exploration of these particles as a potential treatment for other disorders characterized by ROS accumulation, such …

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International Society Of Sports Nutrition Position Stand: Beta-Hydroxy-Beta-Methylbutyrate (Hmb), Jacob M. Wilson, Peter J. Fitschen, Bill Campbell, Gabriel J. Wilson, Nelo Zanchi, Lem Taylor, Colin Wilborn, Douglas S. Kalman, Jeffrey R. Stout, Jay R. Hoffman, Tim N. Ziegenfuss, Hector L. Lopez, Richard B. Kreider, Abbie E. Smith-Ryan, Jose Antonio Jan 2013

International Society Of Sports Nutrition Position Stand: Beta-Hydroxy-Beta-Methylbutyrate (Hmb), Jacob M. Wilson, Peter J. Fitschen, Bill Campbell, Gabriel J. Wilson, Nelo Zanchi, Lem Taylor, Colin Wilborn, Douglas S. Kalman, Jeffrey R. Stout, Jay R. Hoffman, Tim N. Ziegenfuss, Hector L. Lopez, Richard B. Kreider, Abbie E. Smith-Ryan, Jose Antonio

Faculty Bibliography 2010s

Position Statement: The International Society of Sports Nutrition (ISSN) bases the following position stand on a critical analysis of the literature on the use of beta-hydroxy-beta-methylbutyrate (HMB) as a nutritional supplement. The ISSN has concluded the following. 1. HMB can be used to enhance recovery by attenuating exercise induced skeletal muscle damage in trained and untrained populations. 2. If consuming HMB, an athlete will benefit from consuming the supplement in close proximity to their workout. 3. HMB appears to be most effective when consumed for 2 weeks prior to an exercise bout. 4. Thirty-eight mg center dot kg center dot …

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Beta-Hydroxy-Beta-Methylbutyrate Free Acid Reduces Markers Of Exercise-Induced Muscle Damage And Improves Recovery In Resistance-Trained Men, Jacob M. Wilson, Ryan P. Lowery, Jordan M. Joy, Joe A. Walters, Shawn M. Baier, John C. Fuller Jr., Jeffrey R. Stout, Layne E. Norton, Eric M. Sikorski, Stephanie M. C. Wilson, Nevine M. Duncan, Nelo E. Zanchi, John Rathmacher Jan 2013

Beta-Hydroxy-Beta-Methylbutyrate Free Acid Reduces Markers Of Exercise-Induced Muscle Damage And Improves Recovery In Resistance-Trained Men, Jacob M. Wilson, Ryan P. Lowery, Jordan M. Joy, Joe A. Walters, Shawn M. Baier, John C. Fuller Jr., Jeffrey R. Stout, Layne E. Norton, Eric M. Sikorski, Stephanie M. C. Wilson, Nevine M. Duncan, Nelo E. Zanchi, John Rathmacher

Faculty Bibliography 2010s

The purpose of the present study was to determine the effects of short-term supplementation with the free acid form of beta-hydroxy-beta-methylbutyrate (HMB-FA) on indices of muscle damage, protein breakdown, recovery and hormone status following a high-volume resistance training session in trained athletes. A total of twenty resistance-trained males were recruited to participate in a high-volume resistance training session centred on full squats, bench presses and dead lifts. Subjects were randomly assigned to receive either 3 g/d of HMB-FA or a placebo. Immediately before the exercise session and 48 h post-exercise, serum creatine kinase (CK), urinary 3-methylhistadine (3-MH), testosterone, cortisol and …

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Catalytic Nanoceria Are Preferentially Retained In The Rat Retina And Are Not Cytotoxic After Intravitreal Injection, Lily L. Wong, Suzanne M. Hirst, Quentin N. Pye, Christopher M. Reilly, Sudipta Seal, James F. Mcginnis Jan 2013

Catalytic Nanoceria Are Preferentially Retained In The Rat Retina And Are Not Cytotoxic After Intravitreal Injection, Lily L. Wong, Suzanne M. Hirst, Quentin N. Pye, Christopher M. Reilly, Sudipta Seal, James F. Mcginnis

Faculty Bibliography 2010s

Cerium oxide nanoparticles (nanoceria) possess catalytic and regenerative radical scavenging activities. The ability of nanoceria to maintain cellular redox balance makes them ideal candidates for treatment of retinal diseases whose development is tightly associated with oxidative damage. We have demonstrated that our stable water-dispersed nanoceria delay photoreceptor cell degeneration in rodent models and prevent pathological retinal neovascularization in vldlr mutant mice. The objectives of the current study were to determine the temporal and spatial distributions of nanoceria after a single intravitreal injection, and to determine if nanoceria had any toxic effects in healthy rat retinas. Using inductively-coupled plasma mass spectrometry …

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A Compensatory Mutation Provides Resistance To Disparate Hiv Fusion Inhibitor Peptides And Enhances Membrane Fusion, Matthew P. Wood, Amy L. Cole, Piotr Ruchala, Alan J. Waring, Lisa C. Rohan, Preston Marx, Patrick M. Tarwater, Phalguni Gupta, Alexander M. Cole Jan 2013

A Compensatory Mutation Provides Resistance To Disparate Hiv Fusion Inhibitor Peptides And Enhances Membrane Fusion, Matthew P. Wood, Amy L. Cole, Piotr Ruchala, Alan J. Waring, Lisa C. Rohan, Preston Marx, Patrick M. Tarwater, Phalguni Gupta, Alexander M. Cole

Faculty Bibliography 2010s

Fusion inhibitors are a class of antiretroviral drugs used to prevent entry of HIV into host cells. Many of the fusion inhibitors being developed, including the drug enfuvirtide, are peptides designed to competitively inhibit the viral fusion protein gp41. With the emergence of drug resistance, there is an increased need for effective and unique alternatives within this class of antivirals. One such alternative is a class of cyclic, cationic, antimicrobial peptides known as theta-defensins, which are produced by many non-human primates and exhibit broad-spectrum antiviral and antibacterial activity. Currently, the theta-defensin analog RC-101 is being developed as a microbicide due …

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Regulation Of Pten/Akt Pathway Enhances Cardiomyogenesis And Attenuates Adverse Left Ventricular Remodeling Following Thymosin Beta 4 Overexpressing Embryonic Stem Cell Transplantation In The Infarcted Heart, Binbin Yan, Reetu D. Singla, Latifa S. Abdelli, Pawan K. Singal, Dinender K. Singla Jan 2013

Regulation Of Pten/Akt Pathway Enhances Cardiomyogenesis And Attenuates Adverse Left Ventricular Remodeling Following Thymosin Beta 4 Overexpressing Embryonic Stem Cell Transplantation In The Infarcted Heart, Binbin Yan, Reetu D. Singla, Latifa S. Abdelli, Pawan K. Singal, Dinender K. Singla

Faculty Bibliography 2010s

Thymosin beta 4 (T beta 4), a small G-actin sequestering peptide, mediates cell proliferation, migration, and angiogenesis. Whether embryonic stem (ES) cells, overexpressing T beta 4, readily differentiate into cardiac myocytes in vitro and in vivo and enhance cardioprotection following transplantation post myocardial infarction (MI) remains unknown. Accordingly, we established stable mouse ES cell lines, RFP-ESCs and T beta 4-ESCs, expressing RFP and an RFP-T beta 4 fusion protein, respectively. In vitro, the number of spontaneously beating embryoid bodies (EBs) was significantly increased in T beta 4-ESCs at day 9, 12 and 15, compared with RFP-ESCs. Enhanced expression of cardiac …

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Deep Vascular Imaging In Wounds By Two-Photon Fluorescence Microscopy, Ciceron O. Yanez, Alma R. Morales, Xiling Yue, Takeo Urakami, Masanobu Komatsu, Tero A. H. Jarvinen, Kevin D. Belfield Jan 2013

Deep Vascular Imaging In Wounds By Two-Photon Fluorescence Microscopy, Ciceron O. Yanez, Alma R. Morales, Xiling Yue, Takeo Urakami, Masanobu Komatsu, Tero A. H. Jarvinen, Kevin D. Belfield

Faculty Bibliography 2010s

Deep imaging within tissue (over 300 mu m) at micrometer resolution has become possible with the advent of two-photon fluorescence microscopy (2PFM). The advantages of 2PFM have been used to interrogate endogenous and exogenous fluorophores in the skin. Herein, we employed the integrin (cell-adhesion proteins expressed by invading angiogenic blood vessels) targeting characteristics of a two-photon absorbing fluorescent probe to image new vasculature and fibroblasts up to approximate to 1600 mu m within wound (neodermis)/granulation tissue in lesions made on the skin of mice. Reconstruction revealed three dimensional (3D) architecture of the vascular plexus forming at the regenerating wound tissue …

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Predicting The Development Of Counselor Self-Efficacy In Counselors-In-Training During Their First Semester In Practicum Using Embedded, Rich Media In A Distributed Learning Environment., John Super Jan 2013

Predicting The Development Of Counselor Self-Efficacy In Counselors-In-Training During Their First Semester In Practicum Using Embedded, Rich Media In A Distributed Learning Environment., John Super

Electronic Theses and Dissertations

The first semester of practicum is a difficult time for counseling students as they learn to integrate knowledge and theory into clinical practice, often evoking high levels of anxiety (Barbee, Scherer, & Combs, 2003; Ronnestad & Skovholt, 1993) and limiting counselor selfefficacy (Bernard & Goodyear, 2009; Melchert et al., 1996). Practicum is the first opportunity counselors-in-training have to apply theoretical knowledge in a professional setting, use new clinical skills, and test how well they fit into the field of counseling (O‟Connell & Smith, 2005). Additionally, if counselor educators do not fully understand the process counselors in training develop counselor self-efficacy, …

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Habitat Use And Feeding Ecology Of Delphinids Inferred From Stable Isotopes And Fatty Acid Signatures, Nicole Browning Jan 2013

Habitat Use And Feeding Ecology Of Delphinids Inferred From Stable Isotopes And Fatty Acid Signatures, Nicole Browning

Electronic Theses and Dissertations

Prey availability, directly or indirectly, affects all aspects of a predator’s life history and is a primary factor influencing habitat selection and movements. This is especially true for delphinid species where it has been documented that the behaviors and movements of dolphins are strongly influenced by food availability. Unfortunately, the feeding ecology and habitat use patterns of many of these species are poorly understood. Many methodologies that have been employed to explore these facets of dolphin ecology have limitations and constraints or are logistically infeasible. Stable isotope and fatty acid signature analyses have been used extensively on a wide variety …

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Backpacking In The Digital Age: Ethnographic Perspectives From Latin America, Russell Edwards Jan 2013

Backpacking In The Digital Age: Ethnographic Perspectives From Latin America, Russell Edwards

Electronic Theses and Dissertations

My thesis ethnographically examines the changing nature of backpacking for Westerners in Latin America amid a proliferation of mobile computing and social networking. While anthropological and sociocultural research on tourism is extensive, the social scientific literature on backpacking has, thus far, been largely unconcerned with Western Hemisphere countries and the effects of digital technology on this mode of travel. Recent findings suggest, however, that backpacking has currently moved beyond its niche roots as a subculture of independent traveling into a full-fledged tourist industry. My thesis investigates the Latin American backpacking scene to better understand if this is a global trend. …

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Reevaluating The Late Classic Lu-Bat Glyphic Phrase: The Artist And The Underworld, Patrick Carroll Jan 2013

Reevaluating The Late Classic Lu-Bat Glyphic Phrase: The Artist And The Underworld, Patrick Carroll

Electronic Theses and Dissertations

The study of hieroglyphic texts is vital to the interpretation of the ancient Maya and how their worldview contributed to their daily lives. Hieroglyphic decipherment has been an arduous undertaking and a wide variety of the Late Classic Maya writing styles has also been documented. When specific hieroglyphic phrases are not fully understood it has been necessary to utilize other sources of information to help increase the understanding of these texts. The “lubat” glyphic phrase has been utilized in multiple mediums throughout the Late Classic period and is described as an artist’s signature. This artist signature is directly related to …

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Investigative Interviewing: A Team-Level Approach, James Driskell Jan 2013

Investigative Interviewing: A Team-Level Approach, James Driskell

Electronic Theses and Dissertations

To date, the tandem interview approach has yet to be scientifically vetted as an investigative interviewing technique. Specifically, it is unclear what affect the application of two interviewers has on the investigative process. This is alarming considering that this approach is regularly applied under current law enforcement operations. Despite a dearth of research examining the tandem interview approach in investigative interviews, the extensive research on teams would lead us to believe that teams should benefit the overall investigative interview process and outperform individuals in detecting lies. Consequently, the goals of this research were to investigate these potential benefits. Findings from …

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The Development Of An Eco-Gastronomic Tourism (Egt) Supply Chain-Analyzing Linkages Between Farmer, Restaurants, And Tourists In Aruba., Marcelino Kock Jan 2013

The Development Of An Eco-Gastronomic Tourism (Egt) Supply Chain-Analyzing Linkages Between Farmer, Restaurants, And Tourists In Aruba., Marcelino Kock

Electronic Theses and Dissertations

Purpose of this study is to holistically analyze existing linkages between Aruba’s tourism industry, restaurants and local farmers, by examining strategies that constrain the development of linkages between these stakeholders. Previous research indicated that tourism development is often accompanied with increased demand for imported food, which results in foreign exchange leakages, inflation and competition with local production. This phenomenon is very common in the Caribbean, where Aruba, with its heavy reliance on imported goods and services, is no exception to this occurrence. To comprehend potential problems associated with linking both sectors, these in-depth case study addresses three fundamental questions: a) …

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Ancient Maya Stone Polishers And Issues With The Terminology For The Artifacts Polished With These Tools, Rachael Landry Jan 2013

Ancient Maya Stone Polishers And Issues With The Terminology For The Artifacts Polished With These Tools, Rachael Landry

Electronic Theses and Dissertations

The ancient Maya adorned themselves with ornamental objects. This study investigates a type of polishing tool used by the ancient Maya to manufacture certain types of ornaments. Five stone polishing tools used by the ancient Maya are presented and analyzed. Relevant artifact forms are examined to establish which types of artifacts were being polished with these tools. An extensive discussion of the archaeological record and artistic representations of miniature earflares and buttons, which were polished with many of these stone polishing tools, is also included because the terminology used to refer to these objects has varied throughout the academic literature …

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A Clutch, A Pride, A Murder, John Miles Jan 2013

A Clutch, A Pride, A Murder, John Miles

Electronic Theses and Dissertations

A Clutch, A Pride, A Murder is a linked collection of seven short fiction pieces and one novella that examine a world much like our own, but with the cover revealed—a world laid bare, exposed by its desires, its emotions, its beauties, and all its machinations. All of the stories involve, either directly or indirectly, the fictional Ohio city of Milton. Some of the stories take place within this seemingly typical American city, while others only involve characters coming from or in some cases returning to this unassuming location. Regardless, the events of these stories either in cause or effect …

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