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Dietary Effects On The Stoichiometry Of Growth, Regulation, And Wastes Of Ozark Stream Insect Detritivores, Halvor Matthew Halvorson

Graduate Theses and Dissertations

A widespread stressor, anthropogenic nitrogen (N) and phosphorus (P) pollution can increase resource nutrient content and alter animal community composition in freshwater ecosystems. In this dissertation, I used ecological stoichiometry theory to examine effects of diet nutrient content and leaf litter type on growth, regulation, and wastes of aquatic invertebrate detritivores. I tested effects of leaf litter diet carbon:phosphorus (C:P) on growth and stoichiometric regulation of the detritivorous caddisfly Pycnopsyche lepida and used results to determine a threshold elemental ratio of oak litter C:P=1620 that confers peak growth of this species. This empirical, growth-based approach provided a more accurate estimate …

[Re]Visiting The Rime: A Case Study Of Adaptation As Process And Product With The Rime Of The Ancient Mariner, Sally Ferguson

Graduate Theses and Dissertations

This thesis combines adaptation theory with ecology to examine Samuel Taylor Coleridge's Rime of the Ancient Mariner (1798) and its adaptations; it argues further combinations of adaptation with evolutionary theory and ecological ideas could allow for a better interpretation of many texts. The adaptation Rime of the Modern Mariner (2011) by Nick Hayes and the appropriation Perelandra (1943) by C.S. Lewis will also be present in individual chapters to examine the texts' interactions with each other as they evolve and how each work represents the combined theory.

A Watchman On The Walls: Ezekiel And Reaction To Invasion In Anglo-Saxon England, Max K. Brinson

Graduate Theses and Dissertations

During the Viking Age, the Christian Anglo-Saxons in England found warnings and solace in the biblical text of Ezekiel. In this text, the God of Israel delivers a dual warning: first, the sins of the people call upon themselves divine wrath; second, it is incumbent upon God’s messenger to warn the people of their extreme danger, or else find their blood on his hands. This thesis examines how the Anglo-Saxon applied Ezekiel’s warnings to their own cultural crisis. It begins with the early development of this philosophy by the Britons in the 500s, its adoption by the Anglo-Saxons, Irish, and …

Cloning, Fed-Batch Expression And Purification Of A Novel Anti-Candida Peptide And Development Of A Cleavage Resistant Variant Of Green Fluorescence Protein, Rudra Palash Mukherjee

Graduate Theses and Dissertations

This work illustrates that the intelligent design of a bioprocess for the production of peptides of various length is possible by having a prior knowledge of desired product, cell line, fermentation conditions, and choices for downstream recovery. Cloning, expression, and recovery of a novel 12-mer anti-Candida peptide served as an illustrative case for a proposed Escherichia coli platform. The antifungal peptide, expressed as a fusion to GFPUV during high call density fed-batch, was recovered from cell lysate, concentrated using ion exchange chromatography, and finally cleaved with cyanogen bromide. The platform was capable of producing active peptide capable of arresting the …

Influences Of Temperament, Symbolic Gesture, And Caregiver Beliefs On Infant Emotional Expression, Mary Sugg Bassett

Graduate Theses and Dissertations

The purpose of the cross-sectional study was to analyze the relationships of infant temperament, communication through symbolic gesture, caregiver beliefs with emotional expression in infants. Participants were the parents and childcare teachers of sixteen infants and toddlers, between the ages of six and 25 months, currently enrolled at the University of Arkansas child development study center. The independent and combined influence of infant temperament, use of symbolic gestures, and the beliefs of parents and teachers were significantly related to infants’ emotional expression in the study. Parent-reported scores of emotional expression competence were positively correlated to teacher-reported positive temperament levels (r …

Course Delivery Methods And Perceptions Of Self-Efficacy Of Pre-Service Teachers, Susan K. Anselm

Graduate Theses and Dissertations

Conflicting research results regarding outcomes for distance versus face-to-face course delivery methods, as well as a lack of that research, presents a need for further research into distance education. This mixed methods study adds to the existing literature on distance education, and will provide specific research exploring pre-service teachers’ perceptions of their self efficacy in the context of teacher education. This study is descriptive in nature, and has a dual purpose: to add to the existing knowledge base regarding distance education models and traditional face-to-face delivery models in teacher education, and to inform program designers about the impact of these …

Evaluation And Selection Of A Bacillus Based Direct-Fed Microbial Candidate For In Situ Enzyme Production To Improve Gut Health Integrity, Bone Quality And Growth Performance In Poultry, Juan David Latorre Cardenas

Graduate Theses and Dissertations

During the last decade, the increasing interest in renewable energy sources has been changing the distribution of corn utilization from human and animal consumption to biofuel production, leading to a continuous rise in feed costs of livestock diets. Therefore, alternative feed ingredients such as distillers dried grains with solubles (DDGS), as well as cereals like wheat, barley, and sorghum have become part of the feed matrix to maintain or reduce production costs. However, these raw materials often contain a higher concentration of antinutritional factors in comparison to corn, including non-starch polysaccharides (NSP) which increase digesta viscosity and reduce nutrient absorption …

Dissemination And Persistence Of Plasmid Located, Integron Associated Antibiotic Resistant Genes In Wastewater Treatment Plant Effluent And Stream Water Bacteria, _ Suhartono

Graduate Theses and Dissertations

Transmissible plasmid-mediated integrons play important role in the persistence and dissemination of antibiotic resistance throughout the environment. Plasmids from 139 multi-antibiotic resistant Escherichia coli recovered from wastewater treatment plant effluent and upstream and downstream receiving stream water in Northwest Arkansas were extracted and profiled. Genes of class 1 and class 2 integrase (intI), mobilization (mob), sulfamethoxazole resistance (sul), and trimethoprim resistance (dfr) were detected using PCR and confirmed through DNA sequencing. Plasmids from almost half of the isolates (47%) were transmissible with mobF12 gene as the most frequently detected mobilization gene. Plasmid-borne class 1 with and without class 2 integrons …

Development Of Infrared Heating Technology For Corn Drying And Decontamination To Maintain Quality And Prevent Mycotoxins, Shantae Wilson

Graduate Theses and Dissertations

Infrared (IR) heating of corn followed by tempering treatments has potential to rapidly dry and simultaneously decontaminate corn without adverse effect on the overall quality. However, it is vital to determine the optimal processing parameters that maximize throughput and minimize drying energy without affecting overall corn quality. This study investigated the effects of IR heating and tempering treatments on moisture removal, mold load reduction, corn color change, and drying energy requirements. In addition, the study evaluated the feasibility of scaling up IR drying process using a newly built, pilot scale IR dryer. Freshly harvested corn with initial moisture content (IMC) …

Development Of Electrochemical Sensors Suitable For In Vivo Detection For Neurotransmitters, Mengjia Hu

Graduate Theses and Dissertations

The electrochemical method of redox cycling was exploited to achieve new discoveries in neurotransmitter detection and to advance its suitability toward in vivo use. Redox cycling has advantages in signal amplification, selectivity of species based on their electrochemical reaction mechanisms, and limited or no background subtraction. Distinction of dopamine from norepinephrine in a mixture with an electrochemical method at unmodified electrodes was demonstrated for the first time in vitro. This ability resulted from a series of fundamental studies of redox cycling behavior of the catecholamines (dopamine, norepinephrine and epinephrine) using different electrode configurations. Taking advantage of the ECC’ mechanism associated …

"All I Do Is Win": The No-Lose Strategy Of Cafo Regulation Under The Caa, Karl J. Worsham

Journal of Food Law & Policy

Corporate farms, often known as concentrated animal feeding operations ("CAFO'), provide inexpensive animal products but do so by externalizing the cost of their operation in the form of environmental harms and risks to human health. This article explores one possible approach to mitigating CAFO-caused harms. It argues that CAFO regulation under any one of three Clean Air Act ("CAA ") programs will result in net benefits, not just for air quality, but also for other CAFO-caused harms and thus, that CAA regulation of CAFOs is a no-lose strategy. The article then goes further to conclude that, while regulation under any …

Redesigning Gfp Reporter System For Utilization In Clostridium Difficile, Laura E. Fitzgerald

Biological Sciences Undergraduate Honors Theses

Clostridium difficile (C. difficile) is a gram-positive bacterium that comprises part of the healthy human gut microbiome. When it gains sufficient access to peptides, C. difficile flourishes and releases tissue-damaging toxins, which cause inflammation of the colon that can develop into a Clostridium difficile Infection (CDI).¹⁰ The Ivey Laboratory believes that the best tactic in preventing CDIs is stopping peptide ingestion, which theoretically could be accomplished by manipulating the oligopeptide permease (App) system.⁷ In order to verify that altering the App system would successfully impede peptide uptake, first the expression of the app Promoter Region ( …

The Effects Of Smoking On The Calcification Of The Aortic Valve, Brittany Pendergraft

Biological Sciences Undergraduate Honors Theses

Calcific Aortic Valve Disease is responsible for approximately 28,000 deaths annually and the complete mechanism is not completely understood. The disease is known to be effected by an inflammatory response, osteogenic response and an oxidative stress response that leads to thickening and mineralization of the aortic valve. Originally, this disease was thought to be brought on by age and deterioration of the valve but these rapid stress responses when the valve is disrupted by biochemical and mechanical stress has led to its classification as an active disease. Smoking has been shown to have many of the same effects experienced by …

Examining College Student Athlete Attitudes Towards Concussion Testing And Reporting Concussions, Kaitlyn Fry

Health, Human Performance and Recreation Undergraduate Honors Theses

Examining College Student Athlete Attitudes and Behaviors Toward Baseline Neurocognitive Concussion Testing

FryK, Anderson, M, Anderson, M, Schatz, P, Elbin, RJ: University of Arkansas, Fayetteville, Arkansas

Context: Examining athletes’ attitudes toward concussion diagnosis, management, and treatment can lead to improved multi-faceted management of a concussion injury. Although attitudes towards concussion injuries have been studied, the examination of athletes’ attitudes towards baseline computerized neurocognitive testing is understudied and is warranted. Objective: To examine the relationship between sex, concussion history, and previous exposure to baseline testing on athletes’ perceptions of effort provided during baseline testing and the utility of neurocognitive testing. Methods: …

Cfd Model For Ventilation In Broiler Holding Sheds, Christian Heymsfield

Biological and Agricultural Engineering Undergraduate Honors Theses

Broiler production in Arkansas was valued at over $3.6 billion in 2013 (University of Arkansas Extension of Agriculture). Consequently, improvement in any phase of the production process can have significant economic impact and animal welfare implications. From the time poultry leave the farm and until they are slaughtered, they can be exposed to harsh environmental conditions, both in winter and in summer. After road transportation, birds are left to wait in holding sheds once they arrive at the processing plant, for periods of approximately 30 minutes to two hours. This project was interested in this holding shed waiting time during …

Crizotinib And Ceritinib Induce Apoptosis And Necrosis In Primary Rat Hepatocytes With Distinct Capacity, Alec T. Salminen

Biomedical Engineering Undergraduate Honors Theses

Drug development makes up a major portion of biomedical engineering research interests. The FDA oversees the introduction, experimentation, and implementation of all drugs before market approval is granted. Even after market approval is granted, the FDA continues to monitor the safety of all drugs. Crizotinib and ceritinib are two anaplastic lymphoma kinase (ALK) inhibitors recently approved by the FDA. Both drugs are indicated for treatment of non-small cell lung cancers (NSCLC) with abnormal ALK gene, and they are approved with a companion diagnostic test that determines ALK abnormality. Clinical trial data suggest that crizotinib and ceritinib can cause liver injury, …

Optimized Protocol For Measuring 2-Nbdg Uptake As A Cellular Marker Of Glycolytic Demand, Drew Folgmann

Biomedical Engineering Undergraduate Honors Theses

2[N-(7-nitrobenz-2-oxa-1,2-diaxol-4-yl)amino]-2-deoxyglucose (2-NBDG) a fluorescently tagged analog of FDG is ideal for in vitro studies and imaging. 2-NBDG has been proven to be an adequate marker for glucose uptake in many different types of cells [1]. However, across the spectrum of 2-NBDG use a lack of consensus is observed for the following questions. What is the ideal time to fast cells to optimize cellular uptake of 2-NBDG? Also, what is the ideal concentration of 2-NBDG to be used when quantifying glucose uptake? Finally, what is the effect of serum on the uptake of 2-NBDG? To answer these questions and understand glucose …

Exploring The Mechanisms Of Action Of Antifungal Peptides Using Saccharomyces Cerevisiae., Michelle L. Mason

Biological Sciences Undergraduate Honors Theses

Candida albicans is a normal inhabitant of the skin and mucosal membranes of humans, however, in individuals with depressed immune systems or disrupted cutaneous flora, Candida can overgrow and cause serious infection. Candida infection is the fourth leading cause of nosocomial infection in the United States. These infections are often associated with longer hospital stays and higher mortality. Current drug therapies for this infection are largely ineffective due to the increased drug resistance of Candida species, and for some therapeutics, high levels of drug toxicity to humans. Histatin 5 is a naturally occurring salivary peptide that has strong antifungal properties. …

The Influence Of Birth Weight On Body Mass Index In Young Children In Northwest Arkansas, Margaret Frame

The Eleanor Mann School of Nursing Undergraduate Honors Theses

Body Mass Index (BMI) is a screening tool to measure body fat that is calculated based on the height, weight, age, and gender of a child. Being overweight as a child is defined as having a BMI between the 85^th and 95^th percentiles. Obesity is defined as having a BMI at or above the 95^th percentile (“Basics About Childhood Obesity,” 2012). Having a high BMI comes with an increased risk of multiple co-morbidities, both physical and psychosocial (Pulgarón, 2013). Birth weight has been presumed to play a role in the prevalence of obesity in children. The purpose …

Estimating The Frequency Of Sexual Reproduction In The Diatom Stephanodiscus Niagarae, Ann W. Dickens

Biological Sciences Undergraduate Honors Theses

Diatoms are single-celled micro-algae with cell walls composed of silica, that reproduce in a way that results in a decrease in cell size after each round of mitotic (asexual) division. A cell cannot continue to shrink indefinitely, so when the average size of the population reaches a critical threshold, diatoms reproduce sexually and restore their maximal size. It is unclear, however, how frequently diatoms undergo sexual reproduction in nature. As a proxy for sexual reproduction, I monitored changes in cell size of a population of the diatom Stephanodiscus niagarae from the plankton community of Lake Fayetteville (Arkansas, USA). Weekly sampling …

The Effect Of Hypertonicity On Glucose Regulation In Healthy Adults, Alison L. Schroeder

Biological Sciences Undergraduate Honors Theses

Impaired glucose tolerance is the key defining characteristic of diabetes, a condition which affects nearly 30% of Americans. Hydration status and the physiological effects of poor hydration have been linked to compromised glucose tolerance in animal models, but have not been thoroughly investigated in humans. PURPOSE: The purpose of this investigation was to observe the effect of altered hydration status on a healthy male adult’s response to an oral glucose tolerance test (OGTT). METHODS: Four healthy adult men (28.4±1 y, 23.6±1.2 kg/m2, HbA1C 5.6±0.2%, non-smoking, non-athletes) participated. Subjects took OGTT’s in both a hypohydrated and euhydrated state. The euhydrated state …

Damsels In Distress: A Study Of The Effect Of Temperature On Damselfly Competitive Interactions, Madison Pitts

Biological Sciences Undergraduate Honors Theses

Temperature has a fundamental role in shaping numerous physiological aspects of organisms, such as growth rate, that determine an organism's performance. Due to global climate change, temperature is projected to keep increasing. As temperature increases, so does an organism’s growth rate. Also, as competition increases organisms’ growth rate decreases. While studies have examined these factors individually, no experiments have assessed the combined impact of temperature and competition on organism’s growth rates. The experiment we conducted manipulated temperature and density in order to project what will happen as a result of rising temperatures.

The Effects Of Pulmonary Hypertension In Diabetic Zucker Rats On Pulmonary Vascular Contraction And Right Ventricular Size, Michael Franzetti

Biological Sciences Undergraduate Honors Theses

Pulmonary hypertension is a life-threatening disease that is identified by a resting mean pulmonary arterial pressure over 25 mmHg and established by right heart catheterization (Saglam et al., 2015). The symptoms commonly associated with pulmonary hypertension include fatigue, dyspnea, syncope, and chest pain, which severely limit quality of life in diagnosed patients (Saglam et al., 2015). According to recent studies in patients with pulmonary hypertension, the one, three and five year mortality rates are 8%, 25%, and 34%, respectively (Burudpakdee, Shah, Joish, Divers, & Yaldo, 2014). Although some advances have been made in therapies for pulmonary hypertension, the prognosis is …

The Ergogenic Effects Of Acute Citrulline Malate Supplementation On Weightlifting Performance In Trained Females, Lauren Nicole Wethington

Health, Human Performance and Recreation Undergraduate Honors Theses

All previous investigations evaluating the effectiveness of citrulline malate (CM) as an ergogenic aid involved male subjects. The purpose of this investigation was to evaluate the ergogenic effects of CM supplementation on upper- and lower-body, submaximal, resistance exercise performance in trained females. Based on previous results, we hypothesized supplementation with CM would increase performance in females. This study utilized a randomized, double blind, crossover design. Testing trials took place within the Human Performance Laboratory and/or the University of Arkansas: Donna Axum Fitness Center. An a priori sample of 14 subjects were required, therefore this study included 15 female. Subjects reported …

Independent Effect Of Heat Stress During Exercise On Arterial Stiffness, Cash Arcement

Health, Human Performance and Recreation Undergraduate Honors Theses

Context: Cardiovascular disease (CVD) is one of the leading causes of mortality in the United States, accounting for about 1 in every 3 deaths annually. While studies have shown that arterial stiffness, a leading precursor to CVD, improves with passive heat stress not much is known about the independent effect of heat stress during exercise on arterial stiffness. Objective: The objective of this study was to examine the independent effect of heat stress during exercise on arterial stiffness. Design: Participants visited the lab three times; one familiarization and two experimental trials. Experimental trials were randomized and counter-balanced. Setting: All trials …

Examining Different Exercise Types On Falls Efficacy In Older Adults, Hadley Lewis

Health, Human Performance and Recreation Undergraduate Honors Theses

Context: Around 30% of older adults over the age of 65 fall once a year. These falls can result in hospitalization and permanent injury. Falls efficacy, or the confidence level that one will not fall, has been shown to have a direct correlation to the fear of falling and falls. It has been found that 29-92% of older adults who have fallen have a fear of falling and 12-65% of older adults who have not fallen experience a fear of falling. This fear of falling acts as a hindrance on older adult’s ability to complete daily activities and leads …

Rapid, Low-Cost Biosensor For Water-Borne Pathogens In Low-Resource Environments, Kimberly Cribbs

Chemical Engineering Undergraduate Honors Theses

When testing water quality, “microbiological results typically require a minimum of 24 hours to complete” due to reliance on the traditional and inexpensive cultivation method. In 2000, an isothermal DNA amplification method termed loop-mediated isothermal amplification (LAMP) was invented allowing for microbiological assessments to be performed in less than one hour without the need for an expensive thermocycler. In 2013, the World Health Organization reported LAMP as an “attractive diagnostic platform for resource-poor settings: it is fast [15-40 min], isothermal [requiring only a heat block], robust to inhibitors and reaction conditions that usually adversely affect PCR methods, and it generates …

Minimizing Ethanol Concentration In Organosolv Pretreatment For The Saccharification Of Loblolly Pine, Nelson B. Heringer

Biological and Agricultural Engineering Undergraduate Honors Theses

Organic solvent pretreatment, commonly known as organosolv, is a method used to prepare biomass for enzymatic hydrolysis for the production of biofuels. This method common uses ethanol as the organic solvent. However, this creates an economic issue with the product stream, as ethanol becomes a product and input. This project sought to explore how decreasing the use of ethanol in organosolv pretreatment affected the recoverability of sugars after enzymatic hydrolysis. Loblolly pine (Pinus taeda L.) was pretreated at 170 °C for 60 minutes in 1% dilute sulfuric acid and an ethanol concentration varying from 65% to 35%. Compositional …

Municipal Composting And Organic Waste Diversion: The Case Of Fayetteville, Arkansas, Michael E. Hoppe

Biological and Agricultural Engineering Undergraduate Honors Theses

It is estimated that 40% of food is wasted in the United States; representing $165 billion in wasted resources. A vast majority of that wasted food is ultimately placed in landfills where it decomposes and releases harmful greenhouse gases (GHGs). In fact, food waste alone is responsible for 23% of annual methane emissions for the US. This has a huge impact on global climate change due to the potency of methane as a greenhouse gas. Currently only 5% of the food waste produced is recovered across the nation. Source reduction would be the best solution to reducing this food waste, …

Genetic Analysis Of Soybean Mosaic Virus (Smv) Resistance Genes In Soybean [Glycine Max (L.) Merr.], Mariola Klepadlo

Graduate Theses and Dissertations

Soybean mosaic virus (SMV) causes the most serious viral disease in soybean worldwide. Seven SMV strains, G1 - G7, and three independent multi-allelic loci for SMV resistance, Rsv1, Rsv3, and Rsv4, have been identified. In the initial study, 299 soybean germplasm lines were genotyped for Rsv4 region, inoculated with SMV-G1 and G7 strains, and classified into several resistance groups. The Glyma.02g121400 locus was sequenced from ten soybean accessions, and alignment of the sequences revealed three SNPs displaying 100% polymorphic consistency when a soybean genotype carrying the Rsv4 gene was present. A cross between V94-5152 × Lee 68 was made to …