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Leech Parasitism Of The Gulf Coast Box Turtle, Terrapene Carolina Major (Testudines:Emydidae) In Mississippi, Usa, Dennis J. Richardson, C. I. Hammond, W. E. Moser, A. J. Heaton, C. T. Mcallister Jan 2017

Leech Parasitism Of The Gulf Coast Box Turtle, Terrapene Carolina Major (Testudines:Emydidae) In Mississippi, Usa, Dennis J. Richardson, C. I. Hammond, W. E. Moser, A. J. Heaton, C. T. Mcallister

Journal of the Arkansas Academy of Science

Ten leeches were collected from a Gulf Coast box turtle, Terrapene carolina major, found crossing a road in Gulfport, Harrison County, Mississippi, USA. Eight of the leeches were identified as Placobdella multilineata and 2 were identified as Helobdella europaea. This represents the second vouchered report of leeches from a box turtle. Helobdella europaea is reported for the first time associated with a turtle and for the second time from the New World.


Coccidian Parasites (Apicomplexa: Eimeriidae) Of Arkansas Herpetofauna: A Summary With Two New State Records, Chris T. Mcallister, Dagmara Motriuk-Smith, Robert S. Seville, Matthew B. Connior, Stanley E. Trauth, Henry W. Robison Jan 2017

Coccidian Parasites (Apicomplexa: Eimeriidae) Of Arkansas Herpetofauna: A Summary With Two New State Records, Chris T. Mcallister, Dagmara Motriuk-Smith, Robert S. Seville, Matthew B. Connior, Stanley E. Trauth, Henry W. Robison

Journal of the Arkansas Academy of Science

Coccidian parasites (Protista: Apicomplexa: Eimeriidae) commonly infect reptiles, and to a lesser degree, amphibians. The family Eimeriidae includes at least 18 genera and 3 of them, Caryospora, Eimeria, and Isospora have been reported previously from various Arkansas herpetofauna. Over the past 3 decades, our community collaborative effort has provided a great deal of information on these parasites found in amphibians and reptiles of Arkansas. Here, we provide a summary of all coccidians reported from herptiles of the state as well as provide 2 new state records for coccidians from non-native Mediterranean geckos, Hemidactylus turcicus.


Longitudinal Patterns In An Arkansas River Valley Stream: An Application Of The River Continuum Concept, A. A. Burgad, S. T. Clark, M. E. Furr, A. N. Lenard, M. E. Polett, C. D. Robinson, C. R. Sherwood, G. L. Spooner, S. J. Stoughton, S. R. Adams Jan 2017

Longitudinal Patterns In An Arkansas River Valley Stream: An Application Of The River Continuum Concept, A. A. Burgad, S. T. Clark, M. E. Furr, A. N. Lenard, M. E. Polett, C. D. Robinson, C. R. Sherwood, G. L. Spooner, S. J. Stoughton, S. R. Adams

Journal of the Arkansas Academy of Science

The River Continuum Concept (RCC) provides the framework for studying how lotic ecosystems vary from headwater streams to large rivers. The RCC was developed in streams in eastern deciduous forests of North America, but watershed characteristics and land uses differ across ecoregions, presenting unique opportunities to study how predictions of the RCC may differ across regions. Additionally, RCC predictions may vary due to the influence of fishes, but few studies have used fish taxa as a metric for evaluating predictions of the RCC. Our goal was to determine if RCC predictions for stream orders 1 through 5 were supported by …


Academy Presidents And Institutional Members List, Academy Editors Jan 2017

Academy Presidents And Institutional Members List, Academy Editors

Journal of the Arkansas Academy of Science

No abstract provided.


Anti-Cancer And Bioavailabilty Of Arachidin-1 And Arachidin-3 In Colon Cancer Cells, Macy R. Shirley, Cindi Brownmiller, Wing Shun Lam, Sun-Ok Lee Jan 2017

Anti-Cancer And Bioavailabilty Of Arachidin-1 And Arachidin-3 In Colon Cancer Cells, Macy R. Shirley, Cindi Brownmiller, Wing Shun Lam, Sun-Ok Lee

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Cancer is a common cause of death in the United States and locally in the state of Arkansas. Modifiable factors such as tobacco use, physical activity, and diet lead to reduced incidence of colon cancer diagnosis. Plant-based foods may contain phytochemicals that confer health promoting properties. Specifically, peanuts contain phytochemicals known as resveratrol, arachidin-1, and arachidin-3 that have been linked to anticancer activities. However, few research studies have been done on arachidin-1 and arachidin-3 that could develop understanding of their health promoting properties or nutraceutical applications. The objectives of this study were to (1) determine the most effective concentration of …


Arkansas Water Resources Center Annual Technical Report, 2016, Arkansas Water Resources Center Dec 2016

Arkansas Water Resources Center Annual Technical Report, 2016, Arkansas Water Resources Center

Arkansas Water Resources Center Annual Report

No abstract provided.


Mole Street Journal, December 2016, University Of Arkansas, Fayetteville. Dept. Of Chemistry And Biochemistry Dec 2016

Mole Street Journal, December 2016, University Of Arkansas, Fayetteville. Dept. Of Chemistry And Biochemistry

Mole Street Journal

No abstract provided.


Dissecting Salt Tolerance In Soybean By Profiling Differential Physiological Responses Under Salt Stress, Jade Amber Newsome Dec 2016

Dissecting Salt Tolerance In Soybean By Profiling Differential Physiological Responses Under Salt Stress, Jade Amber Newsome

Graduate Theses and Dissertations

Saline soils are common worldwide and limit the yield potential of many crops. Plants respond in a variety of ways to the stress imposed by saline soils. Plants under salt stress must first sense their surroundings and transmit a signal alerting the rest of the plant to the saline conditions. Salt tolerance in soybeans is typically defined by exclusion of chloride ions from foliar tissues. Though differences in ion uptake among soybean genotypes is well documented, the key mechanisms employed by tolerant cultivars to cope with salt stress on the whole-plant level are still largely unknown. Objectives of the current …


The Etiology, Virulence, And Phylogenetics Of The Celery Anthracnose Pathogen, Colletotrichum Fioriniae (= C. Acutatum Sensu Lato), Jason Alexander Pavel Dec 2016

The Etiology, Virulence, And Phylogenetics Of The Celery Anthracnose Pathogen, Colletotrichum Fioriniae (= C. Acutatum Sensu Lato), Jason Alexander Pavel

Graduate Theses and Dissertations

An anthracnose disease of celery, caused by Colletotrichum acutatum sensu lato, has been reported in the U.S. A phylogenetic analysis was conducted on a collection of isolates from celery and non-celery hosts to evaluate their taxonomic position within Colletotrichum acutatum sensu lato. Both celery and non-celery isolates were evaluated for pathogenicity on celery and for vegetative compatibility. Culture filtrates from celery and non-celery isolates were evaluated for their ability to reproduce the unusual leaf curl type symptoms. A total of 18 celery isolates were evaluated for their taxonomic placement based on analysis of the glutamine synthetase intron sequence. All isolates …


Association Mapping Of Seed Weight, Protein, And Sucrose Content; And Kinetics Of Edamame Under Infrared Treatment, Laura Melissa Lara Santisteban Dec 2016

Association Mapping Of Seed Weight, Protein, And Sucrose Content; And Kinetics Of Edamame Under Infrared Treatment, Laura Melissa Lara Santisteban

Graduate Theses and Dissertations

Edamame is a specialty large-seeded soybean (Glycine max L. Merr.) harvested at an immature stage (R6) that has become the second largest consumed soyfood. Although United States is the largest soybean producer, majority of edamame is imported from Asia, highlighting the importance of developing new edamame varieties. Association mapping (AM) provides an alternative to bi-parental linkage mapping method to detect quantitative trait loci (QTL) adding higher resolution and broader germplasm information. Seed weight, sucrose, and protein are quantitative traits of value when selecting edamame lines. However molecular mechanisms controlling each of these traits are still inconclusive and have not been …


How Do Designers Of The Built Environment Attempt To Make Ecological Sustainability Sensory Legible?, Carly L. Bartow Dec 2016

How Do Designers Of The Built Environment Attempt To Make Ecological Sustainability Sensory Legible?, Carly L. Bartow

Architecture Undergraduate Honors Theses

This paper attempts to provide a theoretical framework for making ecosystem function and ecologically sustainable design more perceptible or sensible to people through architecture and the built environment. Design features of the Bertschi School Science Wing and the Bullitt Center in Seattle, Washington are incorporated to illustrate the sensory legibility of ecological sustainability criteria.The criteria are available to designers to help educate a building's occupants on environmentally sustainable design and motivate more sustainable behavior.


Role Of The Inner Shell Architecture On The Various Blinking States And Decay Dynamics Of Core-Shell And Core-Multishell Quantum Dots, Pooja Bajwa Dec 2016

Role Of The Inner Shell Architecture On The Various Blinking States And Decay Dynamics Of Core-Shell And Core-Multishell Quantum Dots, Pooja Bajwa

Graduate Theses and Dissertations

Colloidal semiconductor nanocrystals (quantum dots, QDs) have received much attention in recent years due to their uniquely size-tunable properties leading to a number of promising applications. Some of their most popular applications include their use as fluorescent probes in biology, as electro-optical components and in photovoltaic devices. CdSe-based QDs are particularly important because of their ease of synthesis, high photoluminescence quantum yields (PL QYs) across the whole visible spectrum and their photostabilty. Shelling of core QDs is usually carried out to improve their optical properties, minimize outer environmental effects on their properties, and avoid toxic element exposure to the environment. …


Extracellular Matrix Remodeling And The Inflammatory Response During Skeletal Muscle Regeneration In Sarcopenic Obese Mice, Lemuel Arthur Brown Dec 2016

Extracellular Matrix Remodeling And The Inflammatory Response During Skeletal Muscle Regeneration In Sarcopenic Obese Mice, Lemuel Arthur Brown

Graduate Theses and Dissertations

AIM: Sarocpenic obesity is a national concern within the United States because this metabolic syndrome is tied with reduced mobility and quality of life. Both obesity and aging are associated with insulin-resistance, chronic low-grade inflammation and muscle weakness. Skeletal muscle regeneration is a process that involves the coordinated effort of myogenic regulatory factors (MRFs), inflammatory signaling, and extracellular matrix (ECM) remodeling for optimal regeneration. It has been demonstrated that obesity and aging have a reduction in muscle regeneration. It has not been examined if sarcopenic obesity will further reduce muscle mass and the regenerative process. The purpose of this study …


Quantitative Trait Loci Mapping, Genome-Wide Association Analysis, And Gene Expression Of Salt Tolerance In Soybean, Ailan Zeng Dec 2016

Quantitative Trait Loci Mapping, Genome-Wide Association Analysis, And Gene Expression Of Salt Tolerance In Soybean, Ailan Zeng

Graduate Theses and Dissertations

Salt stress is a common abiotic stress causing yield reduction in soybean. There are differential responses, namely tolerance (excluder) and intolerance (includer), among soybean germplasm. However, the genetic and physiological mechanism for salt tolerance is not clear. Identification of novel QTL for salt tolerance and genes that are differentially expressed under salt stress would help elucidate the salt tolerance mechanism and facilitate the development of salt tolerant cultivars through marker assisted selection (MAS). The objectives of this study were to identify new QTL or genes responsible for salt tolerance using three approaches: QTL mapping, association analysis, and RNA sequencing (RNA-seq). …


Interactions In The Cpsrp Dependent Targeting Of Light Harvesting Chlorophyll Binding Protein To The Thylakoid Membrane, Rory Henderson Dec 2016

Interactions In The Cpsrp Dependent Targeting Of Light Harvesting Chlorophyll Binding Protein To The Thylakoid Membrane, Rory Henderson

Graduate Theses and Dissertations

Targeting of proteins is a critical component of cellular function. A universally conserved targeting system of the cytosol utilizes a signal recognition particle (SRP) to target many proteins contranslationally to the endoplasmic reticulum in eukaryotes or the inner membrane in prokaryotes. A homologous SRP system exists in the chloroplast that delivers light harvesting chlorophyll binding proteins (LHCP) to they thylakoid membrane. The chloroplast SRP (cpSRP) is a heterodimer composed of a novel 43 kDa subunit and a 54 kDa subunit homologous to a component of the SRP system, SRP54. Many details regarding the interactions between the proteins of the cpSRP …


Examination Of Pseudomonas Fluorescence As A Recombinant Expression Host: Cloning, Expression, And Chromatography, Ahmed K.Ali Elmasheiti Dec 2016

Examination Of Pseudomonas Fluorescence As A Recombinant Expression Host: Cloning, Expression, And Chromatography, Ahmed K.Ali Elmasheiti

Graduate Theses and Dissertations

In an effort to expand the pool of bacterium useful for biotechnology applications, Pseudomonas fluorescens, a common gram negative microbe, was examined for its ability to function in a recombinant setting. P. fluorescens is ubiquitous in nature and was initially identified as a soil bacterium found in dirt and is typically associated with plant material. Past literature indicates that it shared characteristics common to Escherichia coli and Bacillus subtilis, including simple growth conditions and potential cloning vectors, providing motivation to look into both the upstream and downstream characteristics of this bacterium. First, it was demonstrated that P. fluorescens could be …


A Study Of Short-Season Winter Cover Crops For Organic High Tunnel Production Systems, Luke Riley Freeman Dec 2016

A Study Of Short-Season Winter Cover Crops For Organic High Tunnel Production Systems, Luke Riley Freeman

Graduate Theses and Dissertations

This two-year study investigated short-season winter cover crops to improve soil quality and growth of subsequent vegetable crops in an organic high tunnel production system. Five winter cover crop treatments including a nontreated control, Austrian winter peas (Pisum arvense), bell beans (Vicia faba), mustard (Brassica juncea cv. Kodiak), and Daikon radish (Raphanus sativus var. longipinnatus) were grown in a high tunnel in a randomized complete block design from mid-November to mid-March, mowed and incorporated into the soil, and followed by a succession of vegetable crops including tomato (Lycopersicon lycopersicum, cv. ‘Plum Dandy’) and broccoli (Brassica oleracea var. italica, cv. ‘Bay …


Moving Towards Harmonization Of The Food Safety Standards: Role Of The Tpp And Ttip Agreements, Ksenia A. Petrovets Dec 2016

Moving Towards Harmonization Of The Food Safety Standards: Role Of The Tpp And Ttip Agreements, Ksenia A. Petrovets

Journal of Food Law & Policy

We are now less dependent on locally available food resources that we have ever been. The continuing industrialization of food production, the advancement in technologies and the rapid development of supply chains granted us the luxury of immediate access to a variety of products originating from local supermarkets all over the world. This, along with the greater level of food production industrialization, inevitably comes the rise of related food safety risks. Because of the enlargement of producing operations, an emerging safety threat in one place may result in a foodborne illness outbreak thousands of miles away from its place of …


Development Of A Semi-Interpenetrating Network Hydrogel To Study The Effects Of Mild Traumatic Brain Injury On Astrocyte Remodeling, Amanda Ederle Dec 2016

Development Of A Semi-Interpenetrating Network Hydrogel To Study The Effects Of Mild Traumatic Brain Injury On Astrocyte Remodeling, Amanda Ederle

Health, Human Performance and Recreation Undergraduate Honors Theses

Traumatic brain injuries (TBIs) are a widespread public health concern affecting over 2.5 million people in the United States alone (1). TBIs are the leading cause of death and disability in children (ages 0-14), and their devastating effects can also be seen in vast population subsets such as professional athletes and combat soldiers (1).

Damaged astrocytes, the specialized glial cells associated with the maintenance of the brain’s environment, respond to injury by altering regulation of certain proteins and ion channels in an attempt to maintain homeostasis (3). Studies have also shown that following a TBI, astrocytes seek to regenerate, a …


Influence Of Ph And Acidic Side Chain Charges On The Behavior Of Designed Model Peptides In Lipid Bilayer Membranes, Venkatesan Rajagopalan Dec 2016

Influence Of Ph And Acidic Side Chain Charges On The Behavior Of Designed Model Peptides In Lipid Bilayer Membranes, Venkatesan Rajagopalan

Graduate Theses and Dissertations

The molecular properties of transmembrane proteins and their interactions with lipids regulate biological function. Of particular interest are interfacial aromatic residues and charged residues in the core helix whose functions range from stabilizing the native structure to regulating ion channels. This dissertation addresses the pH dependence and influence of potentially negatively charged tyrosine, glutamic acid or aspartic acid side chains. We have employed GWALP23 (acetyl-GGALW5LALALALALALALW19LAGA-amide) as favorable host peptide framework. We have substituted W5 with Tyr (Y5GWALP23) and Leu residues with Glu (L12E, L14E or L16E) or Asp (L14D or L16D), and have incorporated specific 2H-labeled alanine residues within the …


Chlorpyrifos Removal For Wastewater Reuse, Indran Kamalanathan Dec 2016

Chlorpyrifos Removal For Wastewater Reuse, Indran Kamalanathan

Chemical Engineering Undergraduate Honors Theses

Approximately 1.2 billion people around the world live in areas of physical water scarcity. This could increase to half of the world’s population by 2030 and could displace 24 to 700 million people unless steps are taken to ensure adequate water supply.1 Water scarcity is an escalating issue within the United States, specifically in Western inland states with arid climates. This scarcity is encouraging communities to investigate tertiary level municipal wastewater treatment, allowing for reuse of wastewater. Unfortunately, wastewater contains numerous contaminants that are not regulated by the Environmental Protection Agency (EPA) under the Safe Drinking Water Act (SDWA). …


Journal Of Food Law & Policy - Fall 2016, Journal Editors Dec 2016

Journal Of Food Law & Policy - Fall 2016, Journal Editors

Journal of Food Law & Policy

No abstract provided.


Selection Of Drought-Tolerant Soybean Lines Using A Field Screening Method And Identification Of Qtls For Slow Wilting And Nitrogen Fixation Associated With Drought-Tolerance, Mirta Beatriz Dalzotto Dec 2016

Selection Of Drought-Tolerant Soybean Lines Using A Field Screening Method And Identification Of Qtls For Slow Wilting And Nitrogen Fixation Associated With Drought-Tolerance, Mirta Beatriz Dalzotto

Graduate Theses and Dissertations

Drought causes significant yield reductions in soybean. The development of drought-tolerant cultivars is an effective alternative to overcome this abiotic stress. Slow wilting, prolonged nitrogen fixation, and minimal yield reduction under water stress play an important role in evaluating breeding materials. Despite advances in knowledge about plant responses to drought conditions, there is little information on effective methodologies for phenotype screening in the field, and some QTLs have been identified for drought tolerance. Field screenings under drought and irrigated conditions are necessary to detect drought tolerant lines. In addition, QTLs and molecular makers associated with drought tolerance traits will greatly …


Examining Denitrification In Agricultural Ditch Sediments Vegetated With Rice Cutgrass (Leersia Oryzoides): Modeling Seasonal Variation Across Increasing Levels Of Nitrate Loading And Model Application To Pre-Existing Datasets, Shannon Lee Speir Dec 2016

Examining Denitrification In Agricultural Ditch Sediments Vegetated With Rice Cutgrass (Leersia Oryzoides): Modeling Seasonal Variation Across Increasing Levels Of Nitrate Loading And Model Application To Pre-Existing Datasets, Shannon Lee Speir

Graduate Theses and Dissertations

Nitrogen (N) derived from fertilizer application in agricultural systems may contribute to significant environmental impacts, including eutrophication of fresh and coastal waters. Rice cutgrass (Leersia oryzoides) can significantly enhance denitrification potential in agricultural ditch sediments, but relationships with known drivers are not well understood. To address this, I examined effects of nitrate (NO3-) availability on dinitrogen gas (N2) and NO3- fluxes seasonally in Chapter 2. Denitrification rates were measured as N2 flux from intact vegetated sediment cores using Membrane Inlet Mass Spectrometry (MIMS). Michaelis-Menten models were developed from observations to mathematically describe N2 fluxes across the spring, summer, and fall …


Quantitative Trait Loci (Qtl) For Salt Tolerance In Soybean And Physiological Response To Salt Stress During Early Growth Stage, Cindy Massiel Lopez Ramirez Dec 2016

Quantitative Trait Loci (Qtl) For Salt Tolerance In Soybean And Physiological Response To Salt Stress During Early Growth Stage, Cindy Massiel Lopez Ramirez

Graduate Theses and Dissertations

Soybean is a major cash crop used as a source of high–quality protein and oil. Salt stress is one of the main abiotic stresses causing significant yield losses in soybean, which is considered a moderately salt–sensitive crop. Breeding selection is a promising strategy to improve salt tolerance as soybean germplasm display wide variation in response to salinity stress. However, the physiological and genetic mechanisms for salt tolerance are not quite clear. The discovery of novel QTL/genes associated with salt tolerance facilitates the development of tolerant cultivars through marker-assisted selection (MAS). The objectives of this study were: 1) identify/confirm QTL associated …


Gastrointestinal Health As A Stimulus For Native American Attraction To Medicinal Asteraceae And Further Implications For Human Evolution, Christopher David Stiegler Dec 2016

Gastrointestinal Health As A Stimulus For Native American Attraction To Medicinal Asteraceae And Further Implications For Human Evolution, Christopher David Stiegler

Graduate Theses and Dissertations

The Asteraceae, or the daisy family, is the largest family of flowering plants in the world, and its ethnobotanical, medical, and economic value is readily apparent cross-culturally. The aim of this thesis is to examine why constituent genera of the Aster family have remained such an integral part of human medicinal plant knowledge, and thereby to reveal any potential physiological, biological, or evolutionary mechanisms underlining human patterns of use regarding the Asteraceae. The present study focuses specifically on Native American plant knowledge made available by the expansive database in the works Daniel Moerman (Moerman 2003). Frequencies of plant use and …


A Bifunctional Nanocomposites Based Electrochemical Biosensor For In-Field Detection Of Pathogenic Bacteria In Food, Meng Xu Dec 2016

A Bifunctional Nanocomposites Based Electrochemical Biosensor For In-Field Detection Of Pathogenic Bacteria In Food, Meng Xu

Graduate Theses and Dissertations

This research focused on the application of electrochemical biosensors for the rapid detection of pathogenic bacteria, Escherichia coli O157:H7 and Salmonella Typhimurium, in foods. The possible presence of pathogenic bacteria in foods has always been a great threat to the wellbeing of people and the revenue of food companies. Therefore, the demand for rapid and sensitive methods to detect foodborne pathogens is growing. In this research, an impedimetric immunosensor was first developed for the rapid detection of E. coli O157:H7 and S. Typhimurium in foods. It was based on the techniques of immunomagnetic separation, enzyme labelling, and electrochemical impedance spectroscopy …


Soil And Plant Property Differences Among High-Yield Soybean Areas In Arkansas, Taylor Cass Adams Dec 2016

Soil And Plant Property Differences Among High-Yield Soybean Areas In Arkansas, Taylor Cass Adams

Graduate Theses and Dissertations

Continued achievement of soybean [Glycine max (L.) Merr.] yields greater than 6719 kg ha-1 (100 bu ac-1) will depend on decreasing the yield gap, which is contingent on gathering more information regarding the soil physical, chemical, and microbiological environment and the main plant factors contributing to high-yield soybean. Therefore, understanding the main factor differences between high- and average-yield areas may provide insight for making management decisions to increase yields. The objectives of this study were i) to evaluate the effects of region and soil depth on soil property differences between high- and average-soybean-yielding areas, ii) to determine which soil properties …


Relationship Among Genetic And Physiological Biomarkers, And Horse Characteristics, Casey Orr Dec 2016

Relationship Among Genetic And Physiological Biomarkers, And Horse Characteristics, Casey Orr

Graduate Theses and Dissertations

The objective of this study was to evaluate relationships among genetic and physiological biomarkers and phenotypic characteristics in horses. Three breeds (Quarter Horse, Thoroughbreds, Miniatures), and five genes of interest (leptin, ghrelin, cytochrome P450, glucocorticoid receptor, and lactate dehydrogenase) were the basis of the investigation. Genomic DNA was extracted from buffy coat samples of Quarter Horses (n = 21), Thoroughbreds (n = 26), and Miniatures (n = 34). Data were analyzed using MIXED MODEL procedures of SAS, with the main effects of breed and SNP genotype. Horse was the experimental unit, and dependent variables were classified as physical, cell differentials, …


Immunotherapeutic Strategies To Reverse Tumor Associated Immunosuppression In Breast Cancer, Sruthi Ravindranathan Dec 2016

Immunotherapeutic Strategies To Reverse Tumor Associated Immunosuppression In Breast Cancer, Sruthi Ravindranathan

Graduate Theses and Dissertations

Heterogenous cancers such as breast carcinoma, would highly benefit from personalized vaccine approaches such as autologous tumor cell vaccines (ATCVs). By using cancer cells that are isolated from self, this approach has the potential to generate a polyclonal immune response against tumor antigens, that are specific to the individual. However, so far, ATCVs against breast cancers have not had any clinical success due to the poor immunogenicity of the malignant cells.

In this study, for the first time, we determined tumor derived G-CSF to play a major role in affecting immune response against breast cancer ATCVs. By using CRISPR/Cas9 genome …